CN109644928A - A kind of intermittent anoxia experimental facility of aquatic animal and its control method - Google Patents

A kind of intermittent anoxia experimental facility of aquatic animal and its control method Download PDF

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Publication number
CN109644928A
CN109644928A CN201910067241.0A CN201910067241A CN109644928A CN 109644928 A CN109644928 A CN 109644928A CN 201910067241 A CN201910067241 A CN 201910067241A CN 109644928 A CN109644928 A CN 109644928A
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CN
China
Prior art keywords
dissolved oxygen
data
experiment parameter
aquatic animal
experimental facility
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CN201910067241.0A
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Chinese (zh)
Inventor
孙永旭
董宏标
张家松
王文豪
谢木娇
李华
刘青松
段亚飞
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South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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Priority to CN201910067241.0A priority Critical patent/CN109644928A/en
Publication of CN109644928A publication Critical patent/CN109644928A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/003Aquaria; Terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/042Introducing gases into the water, e.g. aerators, air pumps

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a kind of intermittent anoxia experimental facility of aquatic animal and its control method, which includes cultivation cabinet;Nitrogen supply (NS) device, for inputting nitrogen to cultivation cabinet;Air supply device is used for cultivation cabinet input air;Input unit;Dissolved oxygen probe, for acquiring the dissolved oxygen data of the water in cultivation cabinet;Controller, the input data for being acquired according to input unit determine that experiment parameter, the experiment parameter include at least two groups dissolved oxygen amount and dissolved oxygen amount corresponding duration;According to the dissolved oxygen data and experiment parameter of dissolved oxygen probe acquisition, the gas transmission rate of nitrogen supply (NS) device and air supply device is controlled.The present invention can need to set according to experiment experiment parameter, and according to the dissolved oxygen data control nitrogen supply (NS) device of dissolved oxygen probe acquisition and the gas transmission rate of air supply device, to reach the experiment parameter of simulation setting, compared with the existing technology, it is more accurate to control.The present invention can be widely applied to experimental instrument field.

Description

A kind of intermittent anoxia experimental facility of aquatic animal and its control method
Technical field
The present invention relates to experimental instrument field, the intermittent anoxia experimental facility of especially a kind of aquatic animal and its controlling party Method.
Background technique
In aquaculture, water body dissolved oxygen fluctuation round the clock, eutrophication, climate change and water body organism decomposition etc. are a variety of Factor can cause water hypoxia.Anoxic ingests to aquatic animal, digest and physiological status etc. has an adverse effect, or even can draw It is dead to play fish body.Therefore, research aquatic animal is of great significance to the response mechanism of anoxic.And in practical anoxic experimental study In, simple Sealing Method or inflated with nitrogen method are often used, water hypoxia is artificially caused.Manual control gas be filled with switch and Rate can only meet the dissolved oxygen fluctuating change in larger range, easily cause the waste of gas, increase cost of labor, and can not essence The dissolved oxygen level of quasi- control experiment water body, lacks system and accurately control operation.
Summary of the invention
In order to solve the above technical problems, it is an object of the invention to: a kind of intermittent anoxic experiment dress of aquatic animal is provided It sets and its control method, accurately to control the dissolved oxygen rate of experimental situation.
The first technical solution adopted by the present invention is:
A kind of intermittent anoxia experimental facility of aquatic animal, comprising:
Cultivate cabinet;
Nitrogen supply (NS) device, for inputting nitrogen to cultivation cabinet;
Air supply device is used for cultivation cabinet input air;
Input unit, for acquiring input data;
Dissolved oxygen probe, for acquiring the dissolved oxygen data of the water in cultivation cabinet;
Controller, the input data for being acquired according to input unit determine that experiment parameter, the experiment parameter include extremely Few two groups of dissolved oxygen amounts and dissolved oxygen amount corresponding duration;According to the dissolved oxygen data and experiment parameter of dissolved oxygen probe acquisition, control The gas transmission rate of nitrogen supply (NS) device and air supply device processed.
Further, block device is equipped in the box for breeding body, if the block device is for cultivating water box to be divided into Dry can carry out the region of water coke slurry.
Further, it is additionally provided in the box for breeding body and pushes away water installations.
Further, it is described according to dissolved oxygen probe acquisition dissolved oxygen data and experiment parameter, control nitrogen supply (NS) device with And the gas transmission rate of air supply device, specifically:
It is poor to be made according to the dissolved oxygen amount in dissolved oxygen data and experiment parameter, using dissolved oxygen data and the difference of dissolved oxygen amount as feeding back Amount;
The ratio of the gas transmission speed of nitrogen supply (NS) device and air supply device is adjusted according to feedback quantity.
It further, further include display device, the display device is mounted on the lateral surface of cultivation cabinet, the controller control Display device processed shows dissolved oxygen data and experiment parameter in current cultivation cabinet.
It further, further include warning device, the dissolved oxygen data-triggered alarm dress that the controller is acquired according to dissolved oxygen probe Set generation alarm signal.
Further, the warning device includes:
Warning lamp, for generating light as alarm signal;
Wireless communication module, for sending alarm signal to the mobile device of user;
Buzzer, for generating sound as alarm signal.
Second of technical solution adopted by the present invention is:
A kind of control method of the intermittent anoxia experimental facility of aquatic animal, comprising the following steps:
Obtain the input data of input unit acquisition;
According to the input data, experiment parameter is determined;The experiment parameter includes at least two groups dissolved oxygen amount and dissolved oxygen amount The corresponding duration;
Obtain the dissolved oxygen data of dissolved oxygen probe acquisition;
According to the dissolved oxygen data and experiment parameter of dissolved oxygen probe acquisition, nitrogen supply (NS) device and air supply dress are controlled The gas transmission rate set.
Further, it is described according to dissolved oxygen probe acquisition dissolved oxygen data and experiment parameter, control nitrogen supply (NS) device with And the gas transmission rate of air supply device, specifically:
It is poor to be made according to the dissolved oxygen amount in dissolved oxygen data and experiment parameter, using dissolved oxygen data and the difference of dissolved oxygen amount as feeding back Amount;
The ratio of the gas transmission speed of nitrogen supply (NS) device and air supply device is adjusted according to feedback quantity.
Further, further comprising the steps of:
The dissolved oxygen data-triggered alarm signal acquired according to dissolved oxygen probe.
The beneficial effects of the present invention are: the present invention can need to set experiment parameter according to experiment, and according to dissolved oxygen probe The dissolved oxygen data control nitrogen supply (NS) device of acquisition and the gas transmission rate of air supply device, to reach the experiment of simulation setting Parameter, compared with the existing technology, it is more accurate to control.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the intermittent anoxia experimental facility of the aquatic animal of specific embodiment of the present invention;
Fig. 2 is a kind of structural schematic diagram of the control cabinet of specific embodiment of the present invention;
Fig. 3 is a kind of circuit module block diagram of the intermittent anoxia experimental facility of the aquatic animal of specific embodiment of the present invention;
Fig. 4 is a kind of process of the control method of the intermittent anoxia experimental facility of the aquatic animal of specific embodiment of the present invention Figure.
Specific embodiment
The present invention is further detailed with specific embodiment with reference to the accompanying drawings of the specification.
Referring to Fig.1, the intermittent anoxia experimental facility of a kind of aquatic animal, including cultivation cabinet 1, nitrogen supply (NS) device 2, sky Gas feeding mechanism 3, input unit 5, warning device 6, monitoring device 7, blocks control cabinet 4 (being equipped with controller in control cabinet) Net device 8 is equipped with the first nanometer aeration disk 11 and the second nanometer aeration disk 12 with water installations 10, cultivation 1 bottom of cabinet is pushed away.First Nanometer aeration disk 11 is connect with nitrogen supply (NS) device 2, and the second nanometer aeration disk 12 is connect with air supply device 3.Cultivate cabinet 1 Inside is fixed with block device 8, and to carry out anoxic treatment to multiple groups experimental animal simultaneously, cultivation 1 bottom of cabinet is equipped with several Water installations 10 are pushed away, are exchanged for enhancing water body in cabinet, cultivation 1 bottom center of cabinet is equipped with drainage hole 13, for experiment to be discharged Water body, monitoring device 7 are connected with several dissolved oxygen probes 9, and dissolved oxygen probe 9 is fixed on inside cultivation cabinet 1, can freely adjust height, For measuring cultivation 1 different location water body dissolved oxygen of cabinet variation.Wherein, monitoring device 7 is equivalent to a hub, will be multiple molten The data summarization of oxygen probe is passed to controller.
Referring to Fig. 2,4 front of control cabinet is equipped with display device 41, for the variation of real-time display water body dissolved oxygen, experiment parameter And the working condition of each element, in order to which user can grasp current experimental state in time.It is equipped with and deposits inside control cabinet 4 Module 42 is stored up, for presetting experiment condition and storage experimental record, the memory module can be hard using flash memory or hard disk etc. Part is realized.Warning lamp 61 is equipped at the top of warning device 6, when warning device 6 receives the alarm command of controller, warning lamp 61 dodges It is bright.Buzzer 62 is equipped with inside warning device 6, when warning device 6 receives the alarm command of controller sending, buzzer 62 is sent out Alarm sound out.Wireless communication module 63 is equipped with inside warning device 6, when the alarm that warning device 6 receives controller sending refers to When enabling, wireless communication module 63 sends warning message to staff's mobile phone.
Referring to Fig. 3, the controller respectively with nitrogen supply (NS) device, air supply device, input unit, dissolved oxygen probe, Warning device is connected with display device.
Wherein, nitrogen supply (NS) device, for inputting nitrogen to cultivation cabinet.
Air supply device is used for cultivation cabinet input air.Oxygen supply device can certainly be used, but it is empty Gas feeding mechanism can greatly save expense without configuring oxygen Dewar bottle.
The nitrogen supply (NS) device and air supply device have included air pump and the Dewar bottle of storage gas.The air pump Working condition can be adjusted according to the control signal of controller, to realize the adjustment to gas transmission speed.
Input unit, for acquiring input data.The input unit can be touch screen, keyboard or speech recognition mould Block etc..
Dissolved oxygen probe, for acquiring the dissolved oxygen data of the water in cultivation cabinet.Water is real-time molten i.e. in measurement cultivation cabinet Oxygen amount.The dissolved oxygen data can be digital signal or analog signal according to the difference of dissolved oxygen probe type selecting.
Controller, the input data for being acquired according to input unit determine that experiment parameter, the experiment parameter include extremely Few two groups of dissolved oxygen amounts and dissolved oxygen amount corresponding duration;According to the dissolved oxygen data and experiment parameter of dissolved oxygen probe acquisition, control The gas transmission rate of nitrogen supply (NS) device and air supply device processed.
In the present embodiment, user can input experiment parameter by input unit, and the dissolved oxygen amount of multiple periods is such as arranged. For example, the dissolved oxygen amount that 20:00-24:00 is arranged is 4.0mg/L, is arranged the 6.0mg/L of the dissolved oxygen amount of 24:00-12:00,12: The dissolved oxygen amount of 00-20:00 is 3.0mg/L.
Then controller can be supplied according to the experiment parameter of present period, i.e. dissolved oxygen amount to control air supply device and nitrogen The gas transmission speed proportional of device, and the dissolved oxygen data acquired according to dissolved oxygen probe are answered, the oxygen content control in cultivating water box is existed Dissolved oxygen amount set by experiment parameter, certainly, in practice, dissolved oxygen amount can float up and down.
Therefore, as preferred embodiment, controller can realize being precisely controlled for dissolved oxygen amount using pid algorithm.Its Specifically:
It is poor to be made according to the dissolved oxygen amount in dissolved oxygen data and experiment parameter, using dissolved oxygen data and the difference of dissolved oxygen amount as feeding back Amount;
The ratio of the gas transmission speed of nitrogen supply (NS) device and air supply device is adjusted according to feedback quantity.
For example, the practical dissolved oxygen data in water body are 3.0mg/L, the dissolved oxygen amount of the experiment parameter set is pressed as 5.0mg/L According to theoretical calculation, the theoretical proportions of the gas transmission speed of air supply device and nitrogen supply (NS) device are N0;At this point, anti-due to existing Feedback amount is -2.0mg/L, needs more air, thus the ratio of gas transmission speed at this time is adjusted to N1, through after a period of time Afterwards, feedback quantity is -1.0mg/L, since feedback quantity absolute value reduces, the ratio of gas transmission speed at this time can be adjusted Whole is N2.Wherein, N1 > N2 > N0.That is the ratio of the gas transmission speed of air supply device and nitrogen supply (NS) device and feedback quantity Size is related.Certainly, N1 and N2 is adjusted on the basis of theoretical proportions N0, it means that firstly the need of according to experiment Parameter calculates theoretical ratio N0, is then obtaining N1 and N2 according to the size of feedback quantity.
Certainly, in practical applications, air supply device and nitrogen supply (NS) device are possible to failure, at them When failure, the dissolved oxygen amount that may result in the water in cultivation cabinet is out of control, leads to the failure of an experiment.In the present embodiment, it controls Device processed can generate alarm command according to whether the dissolved oxygen data of dissolved oxygen probe are below or above danger threshold, make warning device Alert.The danger threshold can determine with experiment parameter set by user, for example, this period of user setting Dissolved oxygen amount is 3.0mg/L, bound of the controller by 3.0mg/L ± 10% as danger threshold.If dissolved oxygen probe is acquired Dissolved oxygen data still exceed danger threshold, controller, which can be determined that, at this time breaks down after continuing one section of setting time.Another In some embodiments, danger threshold is also possible to a unified setting value, for example, no matter experiment parameter set by user such as What, it is 1.0mg/L that controller, which is respectively provided with danger threshold, because under the conditions of this oxygen content, hence it is evident that and aquatic animal is difficult to survive, Once dissolved oxygen amount is lower than danger threshold, immediately alert.
In the present embodiment, experimental animal uses perch, and the control data of intermittent anoxic experiment are as follows: in box for breeding body Perch eupnea dissolved oxygen level is 6.0-7.0mg/L, and water body dissolved oxygen level is 4.0-6.0mg/ under preliminary hypoxemia breathing environment L, water body dissolved oxygen level is 3.0-4.0mg/L under hypoxemia breathing environment, and water body dissolved oxygen level is 2.0- under anaerobic respiration environment 3.0mg/L, the period of eupnea processing are daily 03:00-24:00, and the period of preliminary hypoxemia breathing processing is daily The period of 03:00-03:20,23:40-24:00, hypoxemia breathing processing are daily 03:20-03:40,23:20-23:40, are lacked The period of oxygen breathing processing is daily 0:00-03:40,23:40-0:00.When water body dissolved oxygen level is higher than setting value, then air Input rate reduces, and nitrogen input rate increases, and when water body dissolved oxygen is lower than setting value, then air input rate increases, and nitrogen is defeated Enter rate reduction.Input the water body dissolved oxygen threshold value of each period by input equipment, controller according to the information being collected into Default dissolved oxygen threshold value comparison adjusts air and nitrogen input rate, and issue alarm to warning device and refer to if results abnormity It enables, makes its alert.
Referring to Fig. 4, a kind of control method of the intermittent anoxia experimental facility of aquatic animal is applied in water as shown in Figure 1 On the controller of the intermittent anoxia experimental facility of lively object comprising following steps:
S1, the input data for obtaining input unit acquisition;
S2, according to the input data, determine experiment parameter;The experiment parameter includes at least two groups dissolved oxygen amount and dissolved oxygen Measure the corresponding duration;
S3, the dissolved oxygen data for obtaining dissolved oxygen probe acquisition;
S4, it is supplied according to the dissolved oxygen data and experiment parameter of dissolved oxygen probe acquisition, control nitrogen supply (NS) device and air Answer the gas transmission rate of device.
As preferred embodiment, the step S4 specifically:
S41, made according to the dissolved oxygen amount in dissolved oxygen data and experiment parameter it is poor, using dissolved oxygen data and the difference of dissolved oxygen amount as instead Feedback amount;
S42, adjusted according to feedback quantity nitrogen supply (NS) device and air supply device gas transmission speed ratio.
It is further comprising the steps of as preferred embodiment:
S5, the dissolved oxygen data-triggered alarm signal acquired according to dissolved oxygen probe.
For the step number in above method embodiment, it is arranged only for the purposes of illustrating explanation, between step Sequence do not do any restriction, the execution of each step in embodiment sequence can according to the understanding of those skilled in the art come into Row is adaptively adjusted.
It is to be illustrated to preferable implementation of the invention, but the present invention is not limited to the embodiment above, it is ripe Various equivalent deformation or replacement can also be made on the premise of without prejudice to spirit of the invention by knowing those skilled in the art, this Equivalent deformation or replacement are all included in the scope defined by the claims of the present application a bit.

Claims (10)

1. a kind of intermittent anoxia experimental facility of aquatic animal, it is characterised in that: include:
Cultivate cabinet;
Nitrogen supply (NS) device, for inputting nitrogen to cultivation cabinet;
Air supply device is used for cultivation cabinet input air;
Input unit, for acquiring input data;
Dissolved oxygen probe, for acquiring the dissolved oxygen data of the water in cultivation cabinet;
Controller, the input data for being acquired according to input unit determine that experiment parameter, the experiment parameter include at least two Group dissolved oxygen amount and dissolved oxygen amount corresponding duration;According to the dissolved oxygen data and experiment parameter of dissolved oxygen probe acquisition, nitrogen is controlled The gas transmission rate of gas feeding mechanism and air supply device.
2. the intermittent anoxia experimental facility of a kind of aquatic animal according to claim 1, it is characterised in that: the box for breeding It is equipped with block device in vivo, the block device can carry out the region of water coke slurry for cultivating water box to be divided into several.
3. the intermittent anoxia experimental facility of a kind of aquatic animal according to claim 1, it is characterised in that: the box for breeding It is additionally provided in vivo and pushes away water installations.
4. the intermittent anoxia experimental facility of a kind of aquatic animal according to claim 1, it is characterised in that: described according to molten The dissolved oxygen data and experiment parameter of oxygen probe acquisition, control the gas transmission rate of nitrogen supply (NS) device and air supply device, Its specifically:
It is poor to be made according to the dissolved oxygen amount in dissolved oxygen data and experiment parameter, using dissolved oxygen data and the difference of dissolved oxygen amount as feedback quantity;
The ratio of the gas transmission speed of nitrogen supply (NS) device and air supply device is adjusted according to feedback quantity.
5. the intermittent anoxia experimental facility of a kind of aquatic animal according to claim 1, it is characterised in that: further include display Device, the display device are mounted on the lateral surface of cultivation cabinet, and the controller control display device shows current cultivation case Dissolved oxygen data and experiment parameter in body.
6. the intermittent anoxia experimental facility of a kind of aquatic animal according to claim 1, it is characterised in that: further include alarm Device, the controller generate alarm signal according to the dissolved oxygen data-triggered warning device that dissolved oxygen probe acquires.
7. the intermittent anoxia experimental facility of a kind of aquatic animal according to claim 6, it is characterised in that: the alarm dress It sets and includes:
Warning lamp, for generating light as alarm signal;
Wireless communication module, for sending alarm signal to the mobile device of user;
Buzzer, for generating sound as alarm signal.
8. a kind of control method of the intermittent anoxia experimental facility of aquatic animal, it is characterised in that: the following steps are included:
Obtain the input data of input unit acquisition;
According to the input data, experiment parameter is determined;The experiment parameter includes that at least two groups dissolved oxygen amount and dissolved oxygen amount are corresponding Duration;
Obtain the dissolved oxygen data of dissolved oxygen probe acquisition;
The dissolved oxygen data and experiment parameter acquired according to dissolved oxygen probe, control nitrogen supply (NS) device and air supply device Gas transmission rate.
9. a kind of control method of the intermittent anoxia experimental facility of aquatic animal according to claim 8, it is characterised in that: The dissolved oxygen data and experiment parameter according to dissolved oxygen probe acquisition, control nitrogen supply (NS) device and air supply device Gas transmission rate, specifically:
It is poor to be made according to the dissolved oxygen amount in dissolved oxygen data and experiment parameter, using dissolved oxygen data and the difference of dissolved oxygen amount as feedback quantity;
The ratio of the gas transmission speed of nitrogen supply (NS) device and air supply device is adjusted according to feedback quantity.
10. a kind of control method of the intermittent anoxia experimental facility of aquatic animal according to claim 8, feature exist In: further comprising the steps of:
The dissolved oxygen data-triggered alarm signal acquired according to dissolved oxygen probe.
CN201910067241.0A 2019-01-24 2019-01-24 A kind of intermittent anoxia experimental facility of aquatic animal and its control method Pending CN109644928A (en)

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CN111493002A (en) * 2020-03-19 2020-08-07 菏泽学院 Device for measuring activity and metabolic rate of pelteobagrus vachelli under low oxygen

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Application publication date: 20190419