CN102144580A - Aquatic animal hypoxemia experimental facility and experimental method thereof - Google Patents
Aquatic animal hypoxemia experimental facility and experimental method thereof Download PDFInfo
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- CN102144580A CN102144580A CN2010101069538A CN201010106953A CN102144580A CN 102144580 A CN102144580 A CN 102144580A CN 2010101069538 A CN2010101069538 A CN 2010101069538A CN 201010106953 A CN201010106953 A CN 201010106953A CN 102144580 A CN102144580 A CN 102144580A
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- aquarium
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- seal cover
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to an aquatic animal hypoxemia experimental facility and an experimental method thereof, wherein the content of dissolved oxygen in water of the experimental facility is required to be maintained stably relatively. An aquarium with a glass cover and a water quality analysis device are used in the experimental facility; the experimental facility is characterized in that the aquarium and the water quality analysis device are connected together through a communicating pipe; the opening of the aquarium with a glass cover along the inner side is connected with a seal rubber ring which is matched with a seal cover plane; a plurality of screw holes are distributed on the seal cover plane; each screw hole is screwed in a ventilating insertion pipe with threads; the upper end of the ventilating insertion pipe is provided with a small seal nut. The experimental method comprises the following steps of: before experiment, injecting experimental water into the glass aquarium; putting experimental fish in the water; covering the aquarium with the seal cover provided with the ventilating insertion pipe; presetting the starting number of the ventilating insertion pipes of the seal cover according to the oxygen consumption of experimental fish in unit time; starting the experiment when the dissolved oxygen in the water is stabilized to the experiment-needed low oxygen level; during the experiment, if major change of the dissolved oxygen occurs in the water, taking off a part of screw nuts of the ventilating insertion pipes or increasing the sealing number to adjust the air amount of water.
Description
Technical field
The present invention relates to aquatile experimental provision and test method thereof.
Background technology
The special water body environment that the river mouth mixes with salt water as the fresh water of ocean-intersection, land, the reaction that envirment factor is changed has the susceptibility characteristics.In the last few years, because the influence of mankind's activity, the sharp increase of agrochemical usage amount had aggravated the pollution of Hekou District, made the river mouth have tangible eutrophication trend.Thereby the chemical oxygen consumption (COC) of some eutrophic waters and BOD increase cause local waters anoxic.The dissolved oxygen levels of water body often influences biological growth breeding and reflection pollutional condition, and fish are the dissolved oxygen that satisfies the general needs 6mg/L of growth and breeding demand usually, and crab, shrimp need the above dissolved oxygen of 2-3.5mg/L.Hypoxemia not only can influence the aquatile physiological function, also can influence structure of community and distribute.
When aquatile is in low-oxygen environment, a series of physiological change can take place, low-oxygen environment for simulating nature circle in laboratory, coerce physiological regulation and adaptation mechanism under the state to verify aquatile at hypoxemia, the experimental rig that adopts mainly contains the experimental scheme of throwing in chemical oxygen consumption agent, atoleine sealing or other physical seal modes at present.Three kinds of modes all have close shortcoming: 1, and operation and experimental rig be relative complex all; 2, dissolved oxygen can reduce gradually because of biological respiration consumption in the experimentation, can not keep metastable dissolved oxygen concentration; 3, the Physiological Experiment requirement of incompatibility long period.
Summary of the invention
The technical issues that need to address of the present invention are adjustable the relatively stable of requirement of experiment of keeping of dissolved oxygen content that make the water body of static sealing formula experimental rig.
Technical scheme of the present invention adopts adds a cover glass aquarium, water analysis outfit, utilize Water Test Kits to monitor oxygen in water content in real time, it is characterized in that aquarium and water analysis outfit have communicating pipe to be connected, glass aquarium mouth is connected with the sealing rubber ring that a circle cooperates with the seal cover plane along the inboard, a plurality of screws evenly distribute on the seal cover plane, can increase or reduce the screw number as required, strengthen or dwindle the screw aperture, each screw screws in identical threaded vented cannula respectively, the equipped little nut of sealing in each vented cannula upper end; Earlier test water is injected in the glass aquarium before the experiment, the test fish is put into water, the seal cover of vented cannula is housed well at upper cover of aquarium, unit interval oxygen demand according to the test fish, preestablish the unlatching quantity of seal cover vented cannula, begin formal test when treating in the water body dissolved oxygen stable to the required hypoxemia level of test; In the process of the test, pay close attention to the dissolved oxygen levels that Water Test Kits is monitored, take place than cataclysm as dissolved oxygen in the water body, the little nut that is about to the part vented cannula takes off or increases sealing quantity, to increase or to reduce the water body throughput, it is relatively stable to keep the water body dissolved oxygen; Want between the vented cannula of opening and closing of fault evenly to make the water body ventilation at interval evenly.
The present invention utilizes Water Test Kits to monitor oxygen in water content in real time, and utilize and to open or close encapsulation covers the mode of the little nut of each vented cannula upper end sealing, in the control aquarium water and air mutually the size of breathing area control oxygen in water content.It is simple that the present invention has a method and apparatus, and device good seal performance, easy to operate and can evenly control breathing area flexibly realizes keeping in the water body effect of metastable low-oxygen environment.
Description of drawings
Accompanying drawing is an apparatus structure ideograph of the present invention.
Embodiment
The present invention's (seeing accompanying drawing) adopts glass aquarium 1, water analysis outfit 2, and aquarium has communicating pipe 3 to be connected with water analysis outfit, the lucite seal cover 4 of band breather pipe.Water Test Kits is used for monitoring in real time dissolved oxygen content in the water body, adopts communicating pipe that aquarium and Water Test Kits are coupled together and can avoid installing the influence to the aquatic organism activity of electric wire and probe in aquarium; Glass aquarium mouth is connected with one and encloses the sealing ring plane of sealing rubber ring 5 formations that cooperate with seal cover 4 planes along the inboard, its effect is to put to put the seal cover that has many vented cannula; Seal cover thickness 1.5cm, the identical screw of a plurality of diameters that evenly distributes on the seal cover plane, each screw screws in a threaded vented cannula 6 that diameter is identical respectively, and each vented cannula lower edge flushes and flushes with the water surface that adds in the aquarium, is beneficial to dissolved oxygen; Each vented cannula upper end is equipped with a little nut of sealing respectively, can when needing the adjusting water to contact area with air, little nut be screwed to vented cannula top realize sealing according to the open and close of test needs decision vented cannula, little nut is backed out realized ventilation.
The concrete operations of test: at first test water is injected in the glass aquarium, the test fish is put into water, and adds the seal cover (opening or the little nut of hermetic unit by test dissolved oxygen demand) that assembles at aquarium; Supply the test water body, notice that strict control makes that liquid level is a little more than the seal cover lower edge in the vented cannula, air is all driven out of in the following water body of seal cover this moment.According to the unit interval oxygen demand of test fish, preestablish seal cover and open quantity before the experiment, treat that fish consumes dissolved oxygen and makes in the water body that dissolved oxygen is relatively stable to begin formal test when testing required hypoxemia level; Thereby increase or dwindle the water area that contacts with air and control the dissolved oxygen level of test requirements document with opening or close seal cover.Also can in water body, add the oxygen consumption agent of required dosage in advance, make oxygen in water concentration reduce to the LDO level of test requirements document early.In the process of the test, the level of the dissolved oxygen that the concern Water Test Kits is monitored, take place than cataclysm as dissolved oxygen in the water body, the little nut that is about to the part vented cannula takes off or increases sealing quantity, for making the water body ventilation evenly, try one's best at interval evenly between the little nut of the vented cannula of sealing or unlatching.
Claims (2)
1. aquatic animal hypoxemia experimental provision and test method thereof, the glass aquarium is added a cover in employing, water analysis outfit, utilize Water Test Kits to monitor oxygen in water content in real time, it is characterized in that aquarium and water analysis outfit have communicating pipe to be connected, glass aquarium mouth is connected with the sealing rubber ring that a circle cooperates with the seal cover plane along the inboard, a plurality of screws evenly distribute on the seal cover plane, can increase or reduce the screw number as required, strengthen or dwindle the screw aperture, each screw screws in identical threaded vented cannula respectively, the equipped little nut of sealing in each vented cannula upper end; Earlier test water is injected in the glass aquarium before the experiment, the test fish is put into water, adorn the seal cover of vented cannula well at upper cover of aquarium, unit interval oxygen demand according to the test fish, preestablish the unlatching quantity of seal cover vented cannula, begin formal test when treating in the water body dissolved oxygen stable to the required hypoxemia level of test; In the process of the test, the level of the dissolved oxygen that the concern Water Test Kits is monitored, take place than cataclysm as dissolved oxygen in the water body, soon the little nut of part vented cannula takes off or increases sealing quantity, wants between the vented cannula of sealing or unlatching water body is ventilated evenly.
2. aquatic animal hypoxemia experimental provision according to claim 1 and test method thereof is characterized in that seal cover thickness 1.5cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010106953A CN102144580B (en) | 2010-02-08 | 2010-02-08 | Aquatic animal hypoxemia experimental facility and experimental method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010106953A CN102144580B (en) | 2010-02-08 | 2010-02-08 | Aquatic animal hypoxemia experimental facility and experimental method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102144580A true CN102144580A (en) | 2011-08-10 |
| CN102144580B CN102144580B (en) | 2012-10-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201010106953A Expired - Fee Related CN102144580B (en) | 2010-02-08 | 2010-02-08 | Aquatic animal hypoxemia experimental facility and experimental method thereof |
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| CN (1) | CN102144580B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543241A (en) * | 2013-10-18 | 2014-01-29 | 中国水产科学研究院黄海水产研究所 | Sulfide stress experiment apparatus and experiment method thereof |
| CN105961251A (en) * | 2016-05-25 | 2016-09-28 | 上海海洋大学 | Establishing method and analysis for hypoxia-resistant megalobrama amblycephala |
| CN109644928A (en) * | 2019-01-24 | 2019-04-19 | 中国水产科学研究院南海水产研究所 | A kind of intermittent anoxia experimental facility of aquatic animal and its control method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4324200A (en) * | 1980-02-08 | 1982-04-13 | Rockhill Enterprises | Method and apparatus for growing hydrozoa |
| CN2845446Y (en) * | 2005-11-24 | 2006-12-13 | 王海波 | Oxygen charging type fish bucket |
| CN101138328A (en) * | 2007-10-31 | 2008-03-12 | 上海师范大学 | Fish extreme oxygen deficiency system testing device and test approach thereof |
| CN100518687C (en) * | 2008-03-21 | 2009-07-29 | 中国科学院海洋研究所 | Device for conjunction measuring aquatic animal respiration apocatharsis |
| CN201197318Y (en) * | 2008-04-22 | 2009-02-25 | 谢海燕 | Oxygenated fish tank |
-
2010
- 2010-02-08 CN CN201010106953A patent/CN102144580B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543241A (en) * | 2013-10-18 | 2014-01-29 | 中国水产科学研究院黄海水产研究所 | Sulfide stress experiment apparatus and experiment method thereof |
| CN103543241B (en) * | 2013-10-18 | 2015-07-08 | 中国水产科学研究院黄海水产研究所 | Sulfide stress experiment apparatus and experiment method thereof |
| CN105961251A (en) * | 2016-05-25 | 2016-09-28 | 上海海洋大学 | Establishing method and analysis for hypoxia-resistant megalobrama amblycephala |
| CN105961251B (en) * | 2016-05-25 | 2018-11-06 | 上海海洋大学 | A kind of construction method of lower oxygen concentration resistance megalobrama amblycephala |
| CN109644928A (en) * | 2019-01-24 | 2019-04-19 | 中国水产科学研究院南海水产研究所 | A kind of intermittent anoxia experimental facility of aquatic animal and its control method |
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| Publication number | Publication date |
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| CN102144580B (en) | 2012-10-17 |
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