CN109620750A - A kind of tooth whitening composition itself and its application - Google Patents
A kind of tooth whitening composition itself and its application Download PDFInfo
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- CN109620750A CN109620750A CN201811567094.5A CN201811567094A CN109620750A CN 109620750 A CN109620750 A CN 109620750A CN 201811567094 A CN201811567094 A CN 201811567094A CN 109620750 A CN109620750 A CN 109620750A
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- tooth
- composition itself
- whitening composition
- tooth whitening
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
Abstract
The present invention relates to field of oral care, a kind of tooth whitening composition itself and its application are provided.A kind of tooth whitening composition itself, including protease and protein；The protease can be used as the discoloration Acquired Pellicle of whitening agent removal dental surface, and the protein is insensitive to the protease and can be used as film forming agent in dental surface reconstruction Acquired Pellicle.The present invention also provides a kind of applications of tooth whitening composition itself.
Description
Technical field
The present invention relates to field of oral care more particularly to a kind of tooth whitening composition itself and its applications.
Background technique
Tooth-whitening is a kind of daily demand of people, this demand is also rapid with the continuous improvement that material present is lived
It is promoted.The method of current tooth-whitening mainly includes two kinds, a method of for physical grinding, these products pass through abrasive material, change
Learn or optics preparation presence and work.Polishing uses grinding agent, such as diatomite, silica, calcium pyrophosphate and carbonic acid
Calcium etc..Grinding agent and polishing agent are generally used cooperatively, and remove itself color that one layer of dental enamel shows internal layer enamel, therefore
The intrinsic color of its deep layer is constant, and whitening effect and abrasion tooth degree are presented and be positively correlated.
Second method is to reach whitening effect using chemical reagent, and usually used whitening agent is peroxide.It is this
Method has been demonstrated there is damage to dentine, can destroy the microstructure of dentine, and make the organic substance in dentine
It reduces.Although carrying out the processing of the medical grade of profession, the cold light irradiation whitening for such as acting on peroxide fast onset also can
Temporary hemodia and shouting pain caused by causing dentine to destroy, and ordinary consumer and be unaware of and use hydrogen peroxide
A kind of chemical substance.
It also can achieve the effect of tooth-whitening using protease, however this product is in the presence of the technology for needing to solve at present
Defect.This is because protease is the Acquired Pellicle (Acquired by one layer of browning of removal dental surface covering
Pellicle) reach tooth-whitening purpose, since this layer of Acquired Pellicle also plays important protective effect to tooth, exempt from tooth
It is disseminated in demineralization and by coloring matter in oral cavity, and Acquired Pellicle needs long-term accumulation to can be only achieved with protection function after being destroyed
The thickness of energy.Therefore current techniques using protease tooth-whitening while can make tooth be easier corroded by harmful substance.
To sum up, current tooth-whitening product is by carrying out physically, carry out chemically or in biological structure to tooth
It destroys and realizes that pigment abrades, pigment is bleached and the purpose of degradation pigment attachment.Although consumer may not know these methods pair
The harm of itself, but providing harmless tooth whitening products for consumer is industry urgent problem to be solved.
Summary of the invention
It is an object of the invention to overcome the above-mentioned prior art, a kind of tooth whitening composition itself is provided.
In order to solve the above technical problems, invention uses technical solution as described below.A kind of tooth whitening composition itself, including
Protease and protein；The protease can be used as the discoloration Acquired Pellicle of whitening agent removal dental surface, the protein pair
The protease is insensitive and can be used as film forming agent in dental surface reconstruction Acquired Pellicle.
Preferably, the usage amount of the protease is 300-300000U/g.
Preferably, mass percent shared by the protein is 0.1-90%.
Preferably, the protein is selected from sialoprotein and/or dietary proteins.
Preferably, the sialoprotein is selected from least one of mucin, Statherin and rich proline protein.
Preferably, the dietary proteins are selected from least one of casein, caseinate and ovalbumin.
Preferably, the caseinate is selected from casein sodium, Caseins, potassium salts, calcium caseinate and magnesium caseinate
At least one of.
Above-mentioned tooth whitening composition itself is used to prepare toothpaste, tooth patch, tooth by a kind of application of tooth whitening composition itself
Powder, facing, mouthwash, mouth sprays or chewing gum.
Preferably, the chewing gum is board-like chewing gum, sugar-coat chewing gum or chewing gum lozenge.
The beneficial effects of the present invention are: physics, chemistry and biochemical injury will not be generated to tooth, and can tooth-whitening.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, right combined with specific embodiments below
A specific embodiment of the invention is described in detail.Many details are explained in the following description in order to sufficiently manage
The solution present invention.But the invention can be embodied in many other ways as described herein, those skilled in the art can
To do similar improvement without violating the connotation of the present invention, therefore the present invention is not by the limit of following public specific implementation
System.
Term " protease " refers to the enzyme of hydrolysising peptide key.
Term " prolease activity " or " proteinase activity " mean through being connected to amino acid in hydrolyzed peptide chain
Peptide bond together is catalyzed the proteolytic activity (EC 3.4.21.) of the hydrolysis of amido bond or albumen.For measuring protease activity
Several measurements of property are obtainable in this field.For example " prolease activity measures using People's Republic of China's professional standard
Method " (SB/T 10317-1999) determines proteinase activity.
Term " protein " protein refers to the large biological molecule being made of one or more polypeptide chain, and the peptide chain is by ammonia
Base acid is linked to be by dehydrating condensation, and various amino acid residues arrange in sequence.
Term " sialoprotein " refers to the protein in saliva, this substance based on mucin other protein contents compared with
It is low, therefore also referred to as " saliva mucin ".
Term " dietary proteins " refers to be contained in food, for supplementing the amino acid of needed by human body and the egg of nitrogen
It is white.
Term " mucin " refers to a kind of glycoprotein, it is characterized in that molecular weight is high, high glycosylation is common in nature
Need to provide position of lubrication, such as the knee of people etc..
Term " Statherin " refers to a kind of Phospoprotein for being rich in tyrosine, contain in the saliva of people it is abundant, usually
Also it Pro-rich (see " rich proline protein ") or is existed simultaneously simultaneously with " rich proline protein ".
The Phospoprotein that term " rich proline protein " refers to a kind of Pro-rich, contain in the saliva of people it is abundant,
It is generally also rich in tyrosine (see " rich tyrosine protein ") simultaneously or is existed simultaneously with " rich proline protein ".
It includes cow that term " casein ", which is mammal, the main protein in sheep and people's milk, is a kind of large-scale, hard
Firmly, the curdled milk that fine and close, extremely difficult digestion is decomposed.
Term " caseinate " refers to the additive compound of the metal cations such as casein and sodium, potassium, magnesium or calcium, it is
Casein curdled milk is handled with basic species (such as hydroxide or potassium hydroxide), by the casein of water-insoluble at solubility
A kind of obtained protein-based hydrophilic substance of form.
Term " ovalbumin " refers to one of egg white main protein, is a kind of spherical containing phosphoglucoprotein, molecular weight
For 44.5k Da, the free sulfhydryl group containing there are four is made of 385 amino acid residues.
The present invention relates to a kind of tooth whitening composition itselfs, can be extensive under conditions of not against peroxide or grinding agent
The natural colored of multiple tooth.In particular it relates to use protease as whitening agent, tooth Acquired Pellicle and thereon is removed
Coloured stains, and using protein as film forming agent rebuild Acquired Pellicle.
Specifically, Acquired Pellicle is that tooth stain forms and remove relevant important feature.This structure is by the albumen in saliva
It is formed, and can gradually accumulate and thicken and change colour, have the function of taking care of one's teeth centainly because it is attached to dental surface, together
When be also tooth turn yellow core medium, Acquired Pellicle can be regenerated within 20 minutes after brushing teeth in normal oral it is very one layer thin,
And with time progressive additive.
Specifically, this tooth whitening composition itself can use the form for brightening patch (item/band) to tooth progress whitening.System
The matrix for making dental whitening patch (item/band) is well known in the art.Brightening patch (item/band) can be by as described below thin
Film is made, such as: polyethylene film with a thickness of about 20 to 1500 microns and preferably approximately 50 to 1000 microns.Dry film contains
There are protease whitening agent and protein film forming agent, the protein includes but is not limited to sialoprotein, dietary proteins, glutinous egg
White, Statherin, rich proline protein, casein, caseinate and (or) ovalbumin.Protease is in inactive at this time
State.In use, film is by the saliva soluble protein enzyme whitening agent in oral cavity, thus whitening agent is activated and is discharged into tooth table
Face.In another embodiment, film contains moisture, and protease exists in the form of activating, and is dissolved when use without saliva
Start whitening function rapidly, the rate of dissolution of the whitening agent and the rate for being subsequently released into dental surface can increase by changing
The thickness of white patch (item/band), and the polymer performance, protease whitening agent concentration and the protein film forming agent concentration that are fixed thereon are come
Control, this protease whitening agent concentration is usually from for 300-300000U/g, preferably 500-200000U/g, protein film forming agent
Concentration is usually mass percent 0.1-90%, preferably 1-50%, more preferable 5-20%.
Specifically, the present invention brightens in the form of patch (item/band), ethylene oxide polymer can be used as protease beauty
The carrier of white dose and protein film forming agent, the mixture molecular weight of ethylene oxide polymer is about 10,000 dalton and about
10,000,000 dalton, preferred range are 100,000 to 1,500,000 dalton.This ethylene oxide polymer can be with
Commercially various sources obtain.Specifically, polyoxyethylene can be used in carrier, and molecular weight ranges can be 10,000~1,
000,000 dalton, and specifically, it is preferable to " Polyox " of Union Carbide company.Ethylene oxide polymer accounts in the carrier
Than for including 50-95%, preferably 60-85%.
Specifically, the solvent plasticizer that brightening patch (item/band) can be used can be selected from dihydric alcohol, such as propylene glycol, poly- second
Glycol, can be selected from polyalcohol such as: glycerol, sorbierite, glycerine ester, glycerol triacetate.Solvent is in brightening patch (item/band)
Accounting is 5-30%, preferably 10-25%.
Specifically, patch (item/band) is brightened while can be containing other ingredients of 0.01-2%, such as antioxidant, anti-corrosion
Agent, pH regulator, desensitizer, stabilizer, gelling agent, flavouring agent etc..Preferred gelling agent is carrageenan.Preferred thickener
For hectorite clay, propylene glycol and glycerol.Preferred flavouring natural perfume material is peppermint, spearmint, apricot, cortex cinnamomi etc..Work as application
When responsive type tooth, the patch (item/band) that brightens may include analgesic in favor of being used for this mesh in the use of sensitive group
Ingredient can be ginger-root extract, cimicifuga foetida extract, Cortex Moutan extract, Magnolia cortex P.E.Oral cavity can be added simultaneously
Cleaning agent, preferred mouthwass are alkyl glycosides.Moisturizer, the preferred moisturizer of water retention agent can be added in the present invention
For sorbierite, xylitol.
Specifically, an application form of the invention is whitening tooth cap
With brightening, patch (item/band) is similar, and whitening tooth cap can be used for keeping leaning on using tooth whitening composition itself of the invention
Nearly tooth.
Whitening tooth cap construction, which can be, is conducive to any shape that the present invention implements composition and teeth contact, such as arch
Configuring u slot, the outer wall with bottom and slightly arch form, the two is in conjunction with forming the interior wall of slightly arch shape, between two walls
It can be spaced from each other or be formed through channel-shaped, facing size and shape can be customized according to user.
Currently preferred facing material be it is any can by heat trained shape plastic material, preferred vinyl modeling
Material, because it can carry out moulding processing in lower temperature.However the material that uses of facing pallet of the present invention significantly not office
It is limited to vinyl plastics.Other plastic materials, such as styrol copolymer etc. are equally applicable.
Specifically, it can be used for whitening medical procedure using lightening compositions, wrapped up by the facing containing lightening compositions
The tooth of user is for a period of time and/or several times are to achieve the effect that whitening treatment.
Specifically, the use form of lightening compositions of the invention can be toothpaste or tooth powder.
When based on lightening compositions formulation forms of the invention being toothpaste or tooth powder, composition is forming toothpaste or other pastes
Dosage form formula is needed containing adhesive or thickener.The adhesive of whitening toothpaste of the invention can be carboxy vinyl polymer, angle
Pitch dish glycan, the water soluble salt such as sodium carboxymethylcellulose and carboxy methyl hydroxyethyl fiber of hydroxyethyl cellulose and cellulose ether
Element.In addition, natural gum such as karaya gum, xanthan gum, Arabic gum can also use.Colloidal magnesium aluminum silicate or fine-powdered
Hydrated SiO 2 also is used as achieving the purpose that further to improve lotion for part thickener.Binder/thickener surplus can
Think 0.1-5.0%, preferably 0.1-1%.Ginger-root extract, cimicifuga foetida extract, Cortex Moutan extract, Magnolia cortex P.E can be with
In the composition, reach antianaphylactic effect.Grinding agent can be selected from calcium carbonate, sodium bicarbonate or hydration titanium dioxide in composition
Silicon.
Specifically, one or more sweeteners and flavouring agent may include based on dentifrice composition of the invention.For example, this
Text is above-mentioned to brighten the sweetener and flavouring agent referred in patch (item/band).It can also be comprising being frequently used for cleaning tooth in composition
PH buffer, surfactant, preservative, stabilizer etc..
Specifically, some moisturizing agent materials may include based on dentifrice composition of the invention, to prevent toothpaste to be exposed to
It cannot keep moisture when in air and harden.Moisturizer appropriate is it is well known in the art that the content of moisturizer is 10%-
70%, preferably carrageenan.
Many ingredients identical with dentifrice composition can be used based on dentifrice composition of the invention, dentifrice composition can
To be mixed in drying regime by each component, it is also possible to be mixed into liquid composition, then re-dry is made.Specifically
The composition sprayed of liquid form is nebulized into xeothermic air-flow, is made using various known spray drying technologies by ground
Moisture evaporation only leaves solid component as powder type.It is described that 0.1%- can have based on dentifrice composition of the invention
Moisture between 5%.By the way that water rehydration tooth powder is added or passes through the saliva of user when use.
Specifically, buccal tablet and fuse be can be with form when method of the invention.
It can be based on tablet of the invention solid, can be layering, it is possible to have liquid filled center, it is this
Tablet dissolved was less than 1.5 hours in the time of aqueous solution, it is preferable that less than 15 minutes.Soluble tablet can be but need not
It must be effervescent tablet.
Based on tablet composition moisture content of the invention be 0.4% or smaller, the ingredient of composition further include adhesive,
Composition is sent into formed punch and punch die tabletting pressure by suitable equipment by filler, incremental agent, dyestuff, aromatic and lubricant etc.
It is tight to form tablet.
The following are specific embodiments.
Unless otherwise specified, it prepares solution and tests the experiments such as activity and carried out in 25 DEG C or so of environment.
It is measured the effect of various composition tooth whitenings by following methods in following embodiment.
1, the dyeing of Dense Hydroxyapatite:
It is envisaged for the Dense Hydroxyapatite piece of Tooth-brushing device, cutting is ground as long 30mm, wide 20mm, the standard of thick 4mm
Piece.After cutting washes with water standard film after the completion of grinding, it is soaked in softened water 3 hours.Standard film after natural drying is solid
Due on overflow dyeing machine, overflow dyeing machine is dyed using the mixture of artificial saliva, tealeaves extract and coffee extract, standard film
On overflow dyeing machine among the circulation that dyeing liquor immersion-heat ageing-dyeing liquor impregnates, poststaining is completed within 12 hours, is dried
For use.
2, Tooth-brushing device washing tests:
Required amount of dyeing hydroxy-apatite flag is fixed, leaching is placed in testing combination aqueous solution, uses Tooth-brushing device
After scrubbing 20min, removes after dyeing hydroxy-apatite flag clear water rinses and dried for use immediately.
3, the measurement of tooth whitening effect:
Using whiteness instrument, the whiteness for not carrying out Tooth-brushing device washing tests is measured first, then after measuring and brushing teeth washing tests
Whiteness, difference are the effect of whitening material.
The various anti-oral bacterias of composition adhere to effect and are measured by following methods in following embodiment.
1) the test preparation of oral bacteria
By 35037 microbionation of Streptococcus oralis ATCC in Triptic soya solid medium, training
It supports about 16 hours, rinses lower lawn with DMEM culture medium, bacteria suspension is prepared, until OD600=1.05, for use.
2) Oral Bacteria pollution experiment
By hydroxy-apatite flag to be measured or tooth standard sample bacterial suspension (OD600=1.05；It is equivalent to 108Bacterium/
Ml it) disseminates 30 minutes.
3) elution and count of bacteria
By after dip dyeing hydroxy-apatite flag or tooth standard sample be placed in 0.9%NaCl solution and impregnate, ultrasonic treatment 30
Second, the concussion that is vortexed is further processed 30 seconds, until bacterium is combined to be dispersed.Serial dilution is simultaneously dispersed in pancreas egg peptone Soy Agar plates
On.Bacterium colony counts, and calculating bacterial adhesion number unit is CFU (colony-forming units), calculates the surface area M of sample
(mm2) result expression are as follows:
Bacterial adhesion effect=CFU/M (this numerical value is smaller, and anti-bacterial attachment performance is better)
Embodiment 1
White-skinned face function comparative experiments of the protease to tooth
Prepare respectively containing 1% Bacillus subtilis neutral protease (100,000 U/g), 1% bromelain (800,000 U/g) and
The aqueous solution of 1% papain (600,000 U/g), the hydrogen peroxide with 3%, 6% is according to above-mentioned tooth whitening Efficacy experiments step
It is tested, uses water as negative control, experiment is repeated 3 times, and result shown in following table is average value, and specific experiment result is such as
Under:
1 protease white-skinned face function contrast and experiment of table
Table 1 show the result shows that, three kinds of protease all have the effect of significant tooth-whitening.It is wherein withered containing 1%
The effect of the sample tooth-whitening of vegetable protein enzyme surmounts the effect of low concentration (mass concentration 3%) hydrogen peroxide, and it is double to reach high concentration
The whitening effect of oxygen water (mass concentration 6%).
Embodiment 2
Add the toothpaste tooth-whitening experiment of protease
Basic formulation of tooth-paste is as shown in table 2:
The basic toothpaste composition of table 2
Prepare each addition 1% Bacillus subtilis neutral protease (100,000 U/g), 1% bromelain (800,000 U/
G) the basic toothpaste of papain (600,000 U/g) with 1%, with the hydrogen peroxide basis toothpaste respectively containing 3%, 6% according to above-mentioned
The step of tooth whitening Efficacy experiments, is tested, and uses basic toothpaste as negative control, and experiment is repeated 3 times, shown in following table
As a result it is average value, the results are shown in Table 3 for specific experiment:
Protease white-skinned face function contrast and experiment in 3 toothpaste of table
It is that table 3 is shown the result shows that, three kinds of protease compare basic toothpaste and all have significant tooth-whitening in toothpaste
Gain effect, part protease have better tooth whitening effect compared to low concentration and the hydrogen peroxide of high concentration.
Embodiment 3
Protease, which is used alone, makes tooth be more susceptible to bacterium erosion
Prepare each addition 1% Bacillus subtilis neutral protease (100,000 U/g), 1% bromelain (800,000 U/
G) at split-phase described in table 2 in the basic toothpaste of the papain (600,000 U/g) with 1%, basic toothpaste component and embodiment 2
Together.Three kinds of toothpaste with enzyme and basic toothpaste are tested according to the step of above-mentioned tooth whitening Efficacy experiments, after test
Four groups of hydroxy-apatite flags are tested according to above-mentioned anti-oral bacteria adhesion function experimental procedure respectively again, obtain result such as
Under:
Influence of the independent toothpaste with enzyme of table 4 to bacterial adhesion tooth
It is that table 4 is shown the result shows that, when protease tooth-whitening is used alone, the increase of the quantity of tooth Adherent bacteria,
Illustrating that method comparative basis toothpaste of the protease as tooth-whitening is used alone, the anti-bacterial attachment ability of tooth declines,
Illustrate that protease is used alone as the method for tooth-whitening to be defective, needs to be supplemented with other links.
Embodiment 4
The effect that protein does not have tooth-whitening is used alone
Configuration prepares such as the basic toothpaste in embodiment 2 and adds 5% mucin, 5% egg white egg respectively
White, 5% casein, 5% sialoprotein, 5% dietary proteins, 5% Statherin, 5% rich proline protein and 5% casein
The basic toothpaste of hydrochlorate is tested according to the step of above-mentioned tooth whitening Efficacy experiments, using not adding other compositions basis tooth
Cream is repeated 3 times as negative control, experiment, and result shown in following table is average value, and the results are shown in Table 5 for specific experiment:
Whitening effect of the protein to tooth in 5 toothpaste of table
Shown in table 5 the experimental results showed that, as can promote rebuild Acquired Pellicle some protein, mucin, ovum
Albumin and casein do not increase the whitening effect to tooth after being added into basic toothpaste, illustrate that these albumen are used alone
Matter is to be unable to reach the purpose of tooth-whitening.
Embodiment 5
The gain effect that the present invention has tooth-whitening simultaneously and takes care of one's teeth
Respectively added in basic formulation of tooth-paste 1% Bacillus subtilis neutral protease (100,000 U/g), 1%, using adding
Enzyme toothpaste carries out 9 tooth whitening Efficacy experiments, and the fine and close standard hydroxy-apatite flag after wherein 1 group of whitening is immersed artificial saliva
It takes out and places 20 minutes after liquid 1 minute；Fine and close standard hydroxy-apatite flag after other 8 groups of whitenings is soaked respectively into the artificial saliva of addition
Liquid, 5% mucin, 5% ovalbumin, 5% casein, 5% sialoprotein, 5% dietary proteins, 5% Statherin,
The solution of 5% rich proline protein and 5% caseinate.Above-mentioned same experimentation is carried out using basic toothpaste.To own
Sample carries out oral bacteria adhesion experiment, and the above experiment is repeated 3 times, and the average result of acquisition is as shown in table 6 below:
The gain effect of the whitening of the invention of table 6 and shield tooth
The results show that tooth whiteness is significantly after Validase TSP Concentrate II eliminates Acquired Pellicle shown in table 6
It is promoted, but is easier adherency oral bacteria (number 1), after various protein rebuild Acquired Pellicle rapidly (number 2-9), tooth
Tooth anti-bacterial attachment is significantly promoted than control group (number 1).Very low (the number 10- of toothpaste whitening effect of protease is not added
18).Illustrate method of the protease for using the present invention to use as teeth whitening protein as tooth protectant, there is beauty
White tooth and the double effects to take care of one's teeth.The Acquired Pellicle that tooth is rebuild by protein, compensates for exclusive use protease
Defect.
Embodiment 6
Whitening synergistic effect of the present invention in tooth powder
Basic tooth powder is made of each component of following mass percent:
The basic tooth powder ingredient of table 7
It is above-mentioned basis tooth powder the preparation method is as follows:
1) glycerol measured, Cocoamidopropyl betaine are added to the water and are stirred and evenly mixed, obtain mixed liquor；
2) silica measured, calcium carbonate are mixed in mixing kettle, obtains mixed-powder；
3) essence measured is added in mixed liquor 1)；
4) mixed mixed liquor in 3) is added by sprayer in mixed-powder 2), while starts stirring, it is ensured that liquid
Material is mixed with powder, obtains mixture；
5) 4) mixture is ground by grinder, tooth powder can be obtained after grinding uniformly,
Prepare the basic tooth powder of Bacillus subtilis neutral protease (100,000 U/g) and 5% Statherin of addition 1%；
The basic tooth powder of the bromelain (800,000 U/g) of configuration addition 1% and 5% rich proline protein；The pawpaw of configuration addition 1%
The basic tooth powder of protease (600,000 U/g) and 5% casein sodium.By three kinds of enzyme teeth point and basic tooth powder according to above-mentioned tooth
The step of white-skinned face function is tested is tested, by four groups of hydroxy-apatite flags after test again respectively according to above-mentioned anti-oral bacteria
Adhesion function experimental procedure is tested, and the above experiment is repeated 3 times, and it is as shown in table 8 below to obtain average result:
Table 8 uses tooth powder effect of the invention
It is that table 8 is shown the result shows that, using dentifrice composition comparative basis tooth powder of the invention have it is apparent preferably
The effect of tooth-whitening overcomes destruction of the protease to dental surface using dentifrice composition of the invention, improves tooth
The ability of anti-bacterial attachment.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (9)
1. a kind of tooth whitening composition itself, it is characterised in that: including protease and protein；The protease can be used as whitening agent
The discoloration Acquired Pellicle of dental surface is removed, the protein is insensitive to the protease and can be used as film forming agent in tooth table
Face rebuilds Acquired Pellicle.
2. tooth whitening composition itself as described in claim 1, it is characterised in that: the usage amount of the protease is 300-
300000U/g。
3. tooth whitening composition itself as described in claim 1, it is characterised in that: mass percent shared by the protein is
0.1-90%.
4. tooth whitening composition itself as described in any one of claims 1-3, it is characterised in that: the protein is selected from saliva egg
White and/or dietary proteins.
5. tooth whitening composition itself as claimed in claim 4, it is characterised in that: the sialoprotein is selected from mucin, rich junket
At least one of albumen and rich proline protein.
6. tooth whitening composition itself as claimed in claim 4, it is characterised in that: the dietary proteins are selected from casein, junket egg
At least one of white acid salt and ovalbumin.
7. tooth whitening composition itself as claimed in claim 6, it is characterised in that: the caseinate is selected from caseinic acid
At least one of sodium, Caseins, potassium salts, calcium caseinate and magnesium caseinate.
8. a kind of application of tooth whitening composition itself, it is characterised in that: tooth whitening composition itself described in claim 1 to be used for
Prepare toothpaste, tooth patch, tooth powder, facing, mouthwash, mouth sprays or chewing gum.
9. the application of tooth whitening composition itself as claimed in claim 8, it is characterised in that: the chewing gum is that board-like mouth is fragrant
Sugar, sugar-coat chewing gum or chewing gum lozenge.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA987600A (en) * | 1971-10-13 | 1976-04-20 | Colgate-Palmolive Company | Stabilized toothpaste containing neutral protease |
| US4058596A (en) * | 1971-10-13 | 1977-11-15 | Colgate-Palmolive Company | Stabilized toothpastes containing an enzyme |
| CN101489520A (en) * | 2006-05-25 | 2009-07-22 | Wm.雷格利Jr.公司 | Oral compositions providing enhanced tooth stain removal |
| CN102264242A (en) * | 2008-12-26 | 2011-11-30 | 狮王株式会社 | Chewing gum composition |
| CN105769611A (en) * | 2014-12-23 | 2016-07-20 | 深圳市绿微康生物工程有限公司 | Toothpaste composition capable of strongly eliminating dental plaque |
| CN105963279A (en) * | 2010-01-29 | 2016-09-28 | 高露洁-棕榄公司 | Dental strip for administration of oral treatment |
| CN106636043A (en) * | 2016-12-27 | 2017-05-10 | 广州立白企业集团有限公司 | Preparation method of protease |
-
2018
- 2018-12-19 CN CN201811567094.5A patent/CN109620750A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA987600A (en) * | 1971-10-13 | 1976-04-20 | Colgate-Palmolive Company | Stabilized toothpaste containing neutral protease |
| US4058596A (en) * | 1971-10-13 | 1977-11-15 | Colgate-Palmolive Company | Stabilized toothpastes containing an enzyme |
| CN101489520A (en) * | 2006-05-25 | 2009-07-22 | Wm.雷格利Jr.公司 | Oral compositions providing enhanced tooth stain removal |
| CN102264242A (en) * | 2008-12-26 | 2011-11-30 | 狮王株式会社 | Chewing gum composition |
| CN105963279A (en) * | 2010-01-29 | 2016-09-28 | 高露洁-棕榄公司 | Dental strip for administration of oral treatment |
| CN105769611A (en) * | 2014-12-23 | 2016-07-20 | 深圳市绿微康生物工程有限公司 | Toothpaste composition capable of strongly eliminating dental plaque |
| CN106636043A (en) * | 2016-12-27 | 2017-05-10 | 广州立白企业集团有限公司 | Preparation method of protease |
Non-Patent Citations (8)
| Title |
|---|
| 付丹等: "卵黏蛋白抑菌活性及其作用机制", 《中国食品学报》 * |
| 刘天佳: "《口腔疾病的微生物学基础》", 31 March 1999, 人民卫生出版社 * |
| 姜云涛等: "牙菌斑基质蛋白种类与研究方法进展", 《国际口腔医学杂志》 * |
| 孙建勋等: "牛奶蛋白膜对牙矿化影响的研究进展", 《国际口腔医学杂志》 * |
| 曹家鑫等: "《胶体理论与牙膏生产》", 30 April 1981, 轻工业出版社 * |
| 杜建东等: "《口腔疾病综合诊疗常规 上》", 30 June 2017, 吉林科学技术出版 * |
| 杨萍等: "唾液蛋白质的研究进展", 《国外医学.口腔医学分册》 * |
| 谢慧兰等: "《牙病防治190则》", 31 October 2014, 金盾出版社 * |
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