CN109607767B - Method for accelerating culture of aerobic granular sludge based on NaCl concentration increasing mode - Google Patents
Method for accelerating culture of aerobic granular sludge based on NaCl concentration increasing mode Download PDFInfo
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Abstract
The invention relates to a method for accelerating culture of aerobic granular sludge based on a NaCl concentration increasing mode. The sequencing batch reactor was operated in the manner of water inlet-aeration-settling-drainage with 4 hours per operation period. The invention can obviously accelerate the formation of aerobic granular sludge at lower temperature, shortens the time for fully granulating the flocculent activated sludge to 20-25 days, and solves the problem of overlong culture time of the conventional aerobic granular sludge. The aerobic granular sludge obtained by the method has good stability and high strength, and has COD resistanceCrAnd the ammonia nitrogen has higher removal rate.
Description
Technical Field
The invention relates to a method for accelerating culture of aerobic granular sludge based on a NaCl concentration increasing mode, and belongs to the field of microbial culture.
Background
Granular sludge is a special biofilm of the phenomenon of microbial self-coagulation found in sewage treatment and can be divided into aerobic granular sludge and anaerobic granular sludge. The research of aerobic granular sludge started in 1991, and the aerobic granular sludge has the characteristics of stable structure, compact structure, biological compactness, high biological conversion efficiency, good settling property, strong toxic compound resistance and the like. The aerobic granular sludge has high biomass concentration and has the functions of denitrification and dephosphorization, and compared with the traditional activated sludge method, the aerobic granular sludge can simplify the process flow, reduce the volume and the occupied area of a sewage treatment system and reduce the investment and the operation cost. The aerobic granular sludge can be used for treating various sewage, in particular high-concentration organic sewage, high-salinity sewage and the like.
The problem of long culture time of aerobic granular sludge exists in the practical application process, and most of the reports of the relevant documents of the aerobic granular sludge are as follows: complete granulation generally requires a culture period of 60-80 days. In addition, the aerobic granular sludge culture operation conditions have great influence on the granulation process, the structural characteristics, the degradation function and the like, so that the production and the application of the granular sludge in the actual engineering are directly influenced. Therefore, the aerobic granular sludge is prevented from being applied to a large scale due to a long culture time and the like.
At present, there are reports related to methods for accelerating the culture of aerobic granular sludge, such as: adding a magnetic field to promote the enrichment of iron element in the particles, and adding a chemical reagent with higher cost to promote the aggregation of microorganisms in the granulation process of the sludge. The methods generally have the problems of high cost, difficulty in large-scale engineering application and the like. In addition, the temperature is not controlled in the process of industrially culturing the aerobic granular sludge, and the actual temperature is lower than the proper growth temperature (about 25 ℃) of most microorganisms.
Therefore, a method for accelerating culture of aerobic granular sludge at a lower cost and a lower temperature is needed to reduce the start-up operation time of the aerobic granular sludge process, which is beneficial to the practical engineering application of the process.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for accelerating culture of aerobic granular sludge based on a NaCl concentration increasing mode.
According to the method, the concentration of NaCl is gradually increased, and the granulation of the activated sludge is accelerated at the lower room temperature of 10-20 ℃, so that the granulation of the aerobic sludge is successfully and completely realized within 20-25 days, and the problem that the culture time of the aerobic granular sludge is longer at the lower temperature is solved.
The technical scheme of the invention is as follows:
a method for accelerating culture of aerobic granular sludge based on a NaCl concentration increasing mode comprises the following steps:
(1) adjusting the pH value of the domestic sewage to 7.0-8.5;
(2) inoculating activated sludge into a sequencing batch reactor;
(3) introducing the domestic sewage adjusted in the step (1) from the bottom of the sequencing batch reactor, wherein the sequencing batch reactor operates in a water inlet-aeration-sedimentation-drainage mode, NaCl is added into inlet water during operation to ensure that the concentration of NaCl in the inlet water is 3-4g/L, the concentration is kept for 7 days during operation, then the concentration of NaCl is increased to ensure that the concentration of NaCl in the inlet water is 5-6g/L, the concentration is kept for 7 days during operation, the concentration of NaCl is gradually increased once every 7 days in the mode, and the amplitude of the concentration of NaCl is 2g/L until the concentration of NaCl in the inlet water reaches 9-10 g/L;
(4) the reactor operates for 20-25 days, and granulation of the aerobic sludge is completely realized.
Preferably, in the step (1), the domestic sewage is simulated domestic sewage, and the ammonia nitrogen concentration in the simulated domestic sewage is 40-60 mg/L; CODCrThe concentration is 400-600 mg/L; MgSO (MgSO)4·7H2The concentration of O is 20-30 mg/L; FeSO4·7H2The concentration of 0 is 20-30 mg/L; CaCl2The concentration is 50-70 mg/L; KH (Perkin Elmer)2PO4The concentration is 5-15 mg/L; FeCl3·6H2The concentration of O is 1-2 mg/L; the concentration of EDTA is 15-25 mg/L; KCl concentration is 35-45mg/L, H3BO3The concentration is 0.10-0.30 mg/L; ZnCl2The concentration is 0.10-0.20 mg/L; the concentration of KI is 0.02-0.04 mg/L; CuSO4·5H2The concentration of O is 0.02-0.04 mg/L; NaMoO4·2H2The concentration of O is 0.10-0.25 mg/L; CoCl2·6H2The concentration of O is 0.05-0.06 mg/L; MnCl2·4H2The concentration of O is 0.10-0.25 mg/L; na (Na)2MoO4·4H2The concentration of O is 0.10-0.25 mg/L.
According to the invention, preferably, in step (1), NaHCO is used3And adjusting the pH value of the domestic sewage to 7.5-8.5.
Preferably, in step (2), the activated sludge is activated sludge produced by an aeration tank of a sewage treatment plant.
Preferably, in step (3), the concentration of the inoculated activated sludge is 1.5-2.5g/L, and the added volume accounts for 20-40% of the volume of the reactor.
Preferably, in step (3), the water feeding, aeration, sedimentation and drainage are completed in one cycle, the operation time is 4h, and the operation time is 6 cycles per day.
According to the invention, in the step (3), in the operation period, the water inlet time is 10-15 min, the aeration time is 213-218 min, the settling time is 3-10 min, and the water outlet time is 2-9 min.
According to the invention, in the step (3), the aeration is air aeration, and the aeration rate is 0.12-0.2m3The gas ascending flow speed is 1.0-1.6 cm/s.
Preferably, in step (3), the sequencing batch reactor is operated at a temperature of 10 ℃ to 20 ℃.
Preferably, in step (3), NaCl is added to the feed water to make the NaCl concentration of the feed water be 3-4g/L, the feed water is kept running for 7 days, then the NaCl concentration is increased to make the NaCl concentration of the feed water be 5-6g/L, the feed water is kept running for 7 days, then the NaCl concentration is increased to make the NaCl concentration of the feed water be 7-8g/L, the feed water is kept running for 7 days, and finally the NaCl concentration is increased to 9-10g/L and the feed water is run for 7 days.
The invention has the following advantages:
1. according to the invention, an increasing NaCl concentration mode is adopted, aerobic granular sludge is cultured at a lower room temperature, macroscopic granular sludge is formed in 7-10 days, granulation is completely realized in 20-25 days, and the culturing time for completely realizing granulation of general sludge needs 60-80 days, so that the aerobic sludge granulation process is accelerated, and the problems of difficult formation and overlong formation time of aerobic granular sludge are solved.
2. The invention adopts a NaCl concentration increasing mode to culture aerobic granular sludge, the concentration of the NaCl of the inlet water is increased gradually to greatly increase the hydrophobicity of the sludge, and the repulsive force potential barrier required by the combination of the sludge is greatly reduced, so that the microorganisms in the reactor are easier to gather.
3. After the NaCl concentration of the inlet water is gradually increased, the quality of the outlet water of the sequencing batch reactor fluctuates slightly, and the treatment effect of COD and ammonia nitrogen is slightly reduced, because the salinity is increased to a certain adaptation period for microorganisms, but the concentration of the microorganisms in the reactor is higher, and the quality of the outlet water is gradually improved after 1-2 days, which shows that the promotion effect of NaCl on sludge granulation and microorganism growth begins to appear.
4. The aerobic granular sludge cultured by the method is light yellow, the diameter of the aerobic granular sludge is 1.0-3.0 mm, the sedimentation rate is 30-50 m/h, and the volume number (SVI) of the sludge is5) 20 to 30 mL/g. The microorganisms in the aerobic granular sludge are mainly cocci and also contain some brevibacterium, but filamentous fungi are hardly seen. The granular sludge has compact structure, higher density and strength, better effluent quality and good granule stability.
Drawings
FIG. 1 is a graph showing the change of sludge particles at 25 th day of the operation of the reactor in which NaCl was added in example 1,
FIG. 2 is a graph showing the change in sludge particle at day 25 in a control reactor operated without NaCl addition.
Detailed Description
The present invention will now be described in detail by way of examples with reference to the accompanying drawings. It should be noted that the examples are given solely for the purpose of illustration and are not to be construed as limitations on the scope of the invention, as many insubstantial modifications and variations of the invention may be made by those skilled in the art in light of the above teachings.
The sequencing batch reactor in the embodiment is an existing device and comprises a double-layer sleeve cylindrical reactor consisting of an inner pipe and an outer pipe, the effective volume is 4L, the diameter of the outer pipe is 9.0cm, the height of the outer pipe is 100cm, the diameter of the inner pipe is 8.0cm, the height of the inner pipe is 70cm, and the distance between the inner pipe and the outer pipe is 3 cm. The bottom of the reactor is provided with a water inlet and an aeration port, the upper part of the side wall is provided with a water outlet, the water inlet is connected with a water inlet pipe, the water inlet pipe is provided with a peristaltic pump, the aeration port is connected with an aeration pipeline, air enters the reactor through a micropore aeration head arranged at the center of the bottom of the reactor by the aeration pipeline, the formed air flow pushes liquid and granular sludge in the reactor to move upwards in the inner pipe, then the liquid and the granular sludge move downwards between the inner pipe wall and the outer pipe wall, and the liquid and the granular sludge are pulled by the air flow to move upwards again after reaching the bottom of the reactor, so that the mixed liquid can circularly flow between the inner pipe wall and the outer pipe wall and be uniformly mixed. The water outlet is connected with a water outlet pipe, the water outlet pipe is controlled by a time control switch and an electromagnetic valve, the water outlet is positioned at the position 40cm high of the reactor, the electromagnetic valve is used for controlling the on-off of the water outlet, and the volume exchange rate is 50%. The water inlet, aeration, sedimentation and drainage time of the reactor are automatically controlled by three time relays.
Example 1
A method for accelerating culture of aerobic granular sludge based on a NaCl concentration increasing mode comprises the following steps:
(1) preparation of simulated domestic sewage: preparing simulated domestic sewage to ensure that the ammonia nitrogen concentration in the sewage is 50 mg/L; CODCrThe concentration is 500mg/L, MgSO4·7H2The O concentration is 25 mg/L; FeSO4·7H2The concentration of 0 is 25 mg/L; CaCl2The concentration is 60 mg/L; KH (Perkin Elmer)2PO4The concentration is 10 mg/L; FeCl3·6H2The concentration of O is 1.5 mg/L; EDTA concentration 20 mg/; KCl concentration of 40mg/L, H3BO3The concentration is 0.15 mg/L; ZnCl2The concentration is 0.12 mg/L; the concentration of KI is 0.03 mg/L; CuSO4·5H2The concentration of O is 0.03 mg/L; NaMoO4·2H2The concentration of O is 0.15 mg/L; CoCl2·6H2The concentration of O is 0.058 mg/L; MnCl2·4H2The concentration of O is 0.12 mg/L; na (Na)2MoO4·4H2O concentration of 0.15mg/L, NaHCO3Adjusting the pH value to 7.5-8.5,
(2) inoculating 1L of flocculent aerobic activated sludge into a sequencing batch reactor, wherein the initial sludge concentration (MLSS) in the reactor is 1.5-2.5 g/L;
(3) introducing the domestic sewage adjusted in the step (1) from the bottom of the sequencing batch reactor, operating the sequencing batch reactor in a water inlet-aeration-sedimentation-drainage mode, finishing a water inlet-aeration-sedimentation-drainage mode to form a period, operating the sequencing batch reactor for 4 hours, operating 6 periods every day, operating the sequencing batch reactor for 12 minutes in one period, aerating for 216 minutes, settling for 10 minutes and discharging for 2 minutes, adding NaCl into the inlet water to ensure that the concentration of the NaCl in the inlet water is 4g/L, keeping the concentration for 7 days, then increasing the concentration of NaCl to ensure that the concentration of the NaCl in the inlet water is 6g/L, keeping the concentration for 7 days, then increasing the concentration of NaCl to 8g/L, keeping the concentration for 7 days, and finally increasing the concentration of NaCl to 10g/L for 7 days. After 10 days of culture, aerobic granular sludge begins to appear in the sequencing batch reactor, and flocculent aerobic active sludge appears on the 25 th dayThe sludge is completely changed into yellow aerobic granular sludge, the grain diameter is 0.5-1.5 mm, the suspended solid concentration (MLSS) of the mixed solution is 3.4g/L, and the Sludge Volume Index (SVI)5) The concentration was 26 mL/g. Sequencing batch reactor effluent CODCrThe concentration is 35-80 mg/L, the ammonia nitrogen concentration of the effluent is 0-5.0 mg/L, and the COD isCrThe removal rate is 84-93%, the ammonia nitrogen removal rate is 90-100%, and the obtained aerobic granular sludge has superior treatment performance.
After 21 days of operation, the settling time was reduced to 1 minute in order to remove sludge with poor settling properties. The aeration rate of the reactor was 0.12m3The gas upflow rate was 1.2cm/s, and the reactor was operated at room temperature.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, simplifications, etc., which are made without departing from the spirit and principle of the present invention, should be regarded as being equivalent to the replacement of the above embodiments, and are included in the scope of the present invention.
Comparative example 1
The method for culturing the aerobic granular sludge and the steps are the same as the example 1, except that:
and (4) in the step (3), NaCl concentration is not added in an increasing mode, but NaCl is directly added into the inlet water, so that the NaCl concentration of the inlet water is 5g/L, and the concentration is kept to continuously operate for 25 days.
Comparative example 2
The method for culturing the aerobic granular sludge and the steps are the same as the example 1, except that:
and (4) in the step (3), NaCl concentration is not added in an increasing mode, but NaCl is directly added into the inlet water, so that the NaCl concentration of the inlet water is 10/L, and the concentration is kept to continuously operate for 25 days.
Tests show that after the comparative example 1 and the comparative example 2 are operated for 25 days, aerobic granular sludge cannot be cultured because microorganisms cannot adapt to the sharply increased NaCl concentration.
Claims (6)
1. A method for accelerating culture of aerobic granular sludge based on a NaCl concentration increasing mode comprises the following steps:
(1) adjusting the pH value of the domestic sewage to 7.0-8.5;
(2) inoculating activated sludge into a sequencing batch reactor; the activated sludge is generated by an aeration tank of a sewage treatment plant;
(3) introducing the domestic sewage adjusted in the step (1) from the bottom of the sequencing batch reactor, operating the sequencing batch reactor in a water inlet-aeration-sedimentation-drainage mode, adding NaCl into inlet water to ensure that the concentration of the NaCl in the inlet water is 3-4g/L, keeping the concentration for 7 days, then increasing the concentration of the NaCl to ensure that the concentration of the NaCl in the inlet water is 5-6g/L, keeping the concentration for 7 days, then increasing the concentration of the NaCl to ensure that the concentration of the NaCl in the inlet water is 7-8g/L, keeping the concentration for 7 days, and finally increasing the concentration of the NaCl to 9-10g/L for 7 days; the concentration of the inoculated activated sludge is 1.5-2.5g/L, and the adding volume accounts for 20-40% of the volume of the reactor; the operating temperature of the sequencing batch reactor is 10-20 ℃;
(4) after NaCl is added, the reactor operates for 20-25 days, and granulation of the aerobic sludge is completely realized.
2. The method for accelerating the culture of the aerobic granular sludge based on the NaCl concentration increasing mode as claimed in claim 1, wherein in the step (1), the domestic sewage is simulated domestic sewage, and the ammonia nitrogen concentration in the simulated domestic sewage is 40-60 mg/L; CODCrThe concentration is 400-600 mg/L; MgSO (MgSO)4•7H2The concentration of O is 20-30 mg/L; FeSO4•7H2The concentration of 0 is 20-30 mg/L; CaCl2The concentration is 50-70 mg/L; KH (Perkin Elmer)2PO4The concentration is 5-15 mg/L; FeCl3•6H2The concentration of O is 1-2 mg/L; the concentration of EDTA is 15-25 mg/L; KCl concentration is 35-45mg/L, H3BO3The concentration is 0.10-0.30 mg/L; ZnCl2The concentration is 0.10-0.20 mg/L; the concentration of KI is 0.02-0.04 mg/L; CuSO4•5H2The concentration of O is 0.02-0.04 mg/L; NaMoO4•2H2The concentration of O is 0.10-0.25 mg/L; CoCl2•6H2The concentration of O is 0.05-0.06 mg/L; MnCl2•4H2The concentration of O is 0.10-0.25 mg/L; na (Na)2MoO4•4H2The concentration of O is 0.10-0.25 mg/L.
3. The method for accelerating the culture of aerobic granular sludge based on the NaCl concentration increasing mode as claimed in claim 1, wherein in the step (1), NaHCO is adopted3And adjusting the pH value of the domestic sewage to 7.5-8.5.
4. The method for accelerating the culture of aerobic granular sludge based on the NaCl concentration increasing mode as claimed in claim 1, wherein in the step (3), the water feeding, the aeration, the sedimentation and the water discharging are completed in one cycle, the operation is performed for 4 hours in one cycle, and the operation is performed for 6 cycles every day.
5. The method for accelerating the culture of the aerobic granular sludge based on the NaCl concentration increasing mode as claimed in claim 1, wherein in the step (3), during the operation period, the water inlet time is 10-15 min, the aeration time is 213-218 min, the sedimentation time is 3-10 min, and the water outlet time is 2-9 min.
6. The method for accelerating the culture of aerobic granular sludge based on the NaCl concentration increasing mode as claimed in claim 1, wherein the aeration in the step (3) is air aeration with an aeration rate of 0.12 to 0.2m3The gas ascending flow speed is 1.0-1.6 cm/s.
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| CN114656032A (en) * | 2022-04-25 | 2022-06-24 | 嘉兴学院 | Method for strengthening granulation of salt-tolerant aerobic sludge by adding anaerobic particles |
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