CN109601779A - A kind of bee raising cold-resistant biological bacteria preparation, preparation method and applications - Google Patents
A kind of bee raising cold-resistant biological bacteria preparation, preparation method and applications Download PDFInfo
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- CN109601779A CN109601779A CN201910009691.4A CN201910009691A CN109601779A CN 109601779 A CN109601779 A CN 109601779A CN 201910009691 A CN201910009691 A CN 201910009691A CN 109601779 A CN109601779 A CN 109601779A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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Abstract
The invention discloses a kind of bee raising cold-resistant biological bacteria preparation, preparation method and applications, which includes 1-8 parts of Gilliamella bacterium, 1-6 parts of glycerol, 1-10 parts of yeast powder and excess water.Cold-resistant biological bacteria preparation provided by the invention can effectively help honeybee overwintering, improve the survival rate of the Wintering Period of honeybee.
Description
Technical field
The present invention relates to bee raising preparation technique field, and in particular to a kind of bee raising cold-resistant biological bacteria system
Agent, preparation method and applications.
Background technique
China is that the first in the world bee-keeping big country and honey produce the first big country.In order to accelerate the development of bee-keeping industry, I
State is recently proposed the strategy for making great efforts to be promoted the added value of China's bee product quality safety level and bee product, the proposition gesture of the policy
It must lead to the minimized of antibiotic usage during beekeeping.The use of probiotics can reduce antibiotic and Related Drug
The use of object, to guarantee that bee product safety, the quality for improving bee product all have important role.
In the north in China, honeybee is required to 3-5 months Wintering Periods of experience, and overwintering preparatory stage beekeeper is necessary for bee colony feeding
Enough overwintering feed are fed, but due to the influence of many factors, occur a large amount of the loss even phenomenon of bee colony death after overwintering.
For this purpose, we have developed a kind of cold-resistant biological bacteria preparation of honeybee Wintering Period application.
Summary of the invention
The purpose of the present invention is to provide a kind of bee raising cold-resistant biological bacteria preparation, preparation method and applications, institutes
The cold-resistant biological bacteria preparation that the honeybee Wintering Period of offer uses can effectively help honeybee overwintering, improve the survival of Wintering Period honeybee
Rate.
The present invention solves its technical problem and adopts the following technical solutions to realize.
The present invention provides a kind of bee raising cold-resistant biological bacteria preparations, comprise the following components in parts by weight:
1-8 parts of Gilliamella bacterium, 1-6 parts of glycerol, 1-10 parts of yeast powder and 80-100 parts of water.
The present invention also provides a kind of preparation methods of above-mentioned bee raising cold-resistant biological bacteria preparation, including following step
It is rapid:
Gilliamella bacterium, glycerol, yeast powder, carbohydrate, amino acid and water are stirred, sealed after high pressure sterilization,
Bee raising cold-resistant biological bacteria preparation is made.
The present invention also provides a kind of applications of above-mentioned bee raising cold-resistant biological bacteria preparation, by cold-resistant biological bacteria preparation
It makes an addition in the overwintering feed of honeybee.
The beneficial effects of the present invention are:
The present invention provides a kind of bee raising cold-resistant biological bacteria preparations, preparation method and applications, use the present invention
In bee raising the honeybee of Wintering Period is fed with cold-resistant biological bacteria preparation, increase the cold tolerance of honeybee, bee can be improved
The overwintering success rate of group reduces bee colony loss, accelerates the rejuvenation speed of coming year bee colony.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Below to a kind of bee raising cold-resistant biological bacteria preparation, preparation method and applications provided in an embodiment of the present invention
It is specifically described.
The embodiment of the present invention provides a kind of bee raising cold-resistant biological bacteria preparation, comprises the following components in parts by weight:
1-8 parts of Gilliamella bacterium, 1-6 parts of glycerol, 1-10 parts and 80-100 parts of yeast powder.
The embodiment of the present invention provides a kind of bee raising cold-resistant biological bacteria preparation, including following components: Gilliamella
Bacterium, glycerol, yeast powder and water.Wherein, Gilliamella bacterium and glycerol not only provide the demand that honeybee resists low temperature, improve
Overwintering survival rate, and contain yeast powder, while providing nutriment abundant, the conversion of overwintering feed can also be accelerated,
Conducive to the utilization speed of honeybee, wherein
Gilliamella bacterium: Gilliamella bacterium is that the honeybee bacterium of discovery in 2012 newly belongs to, and belongs to Orbacea section.
Centering bee winter is with the distribution of summer intestinal microflora studies have shown that Gilliamella bacterium is the intracorporal dominant bacteria of honeybee
Group, is microorganism most in honeybee enteron aisle.From summer to winter, the ratio of Gilliamella bacterium is by 39.48% in honeybee body
Rise to 52.67%, should the result shows that, Gilliamella bacterium honeybee keep out the cold aspect play an important role.
Yeast powder: protein content is higher, is 52%, without containing fat, various amino acid contents are higher, it is seen that yeast powder
Good protein is only provided, amino acid content is high, also contains inorganic salts and vitamin.Yeast is added in cold-resistant biological bacteria preparation
Powder does not only have advantageous effect in terms of honeybee supplement protein feed, promotes the breeding of bee colony, improve their production
Power increases honey yield, and can accelerate the conversion of overwintering feed, conducive to the quick utilization of bee colony.Currently, in current bee raising
In the process, overwintering preceding beekeeper needs to add overwintering feed into bee colony, and the main component of overwintering feed is white granulated sugar: water=2: 1.
And yeast powder can convert white granulated sugar to inverted sugar and glucose, reduce the intracorporal conversion process of honeybee, save honeybee physical strength,
So that the safety of honeybee is passed the winter.
Glycerol: glycerol is cryoprotectant in generally acknowledged insect bodies as a kind of polyalcohol.A large amount of products of glycerol in insect bodies
It is tired to help to maintain morphosis and physiological function of the cell under low-temperature condition.Pre-stage test also indicates that sweet at low ambient temperatures
Bee resists injury of the low temperature to itself human body by accumulation glycerol.
In some embodiments, bee raising cold-resistant biological bacteria preparation, comprises the following components in parts by weight:
2-5 parts of Gilliamella bacterium, 1-3 parts of glycerol, 1-3 parts and 90-100 parts of yeast powder.
In some embodiments, the concentration of Gilliamella bacterium is 3 × 108/ part.
In some embodiments, bee raising cold-resistant biological bacteria preparation further includes nutriment, and nutriment includes
1-10 parts and -6 parts of amino acid 1 of carbohydrate.
In some embodiments, carbohydrate include in glucose, sucrose, fructose, maltose and soluble starch extremely
Few one kind, preferably glucose.
In some embodiments, amino acid include proline, alanine, serine, tyrosine, arginine, lysine,
At least one of threonine, valine and glutamic acid, preferably proline.
Bee raising provided in an embodiment of the present invention cold-resistant biological bacteria preparation, further includes nutriment, such as carbohydrate and ammonia
Base acid, glucide is as main energy source, and amino acid may be converted into energy when honeybee encounters Cold stress
Or other cold-resistant substances are converted into improve the winter resistance of human body.
As it can be seen that the embodiment of the present invention provides a kind of bee raising cold-resistant biological bacteria preparation, with Gilliamella bacterium, sweet
Oil, yeast powder and suitable quantity of water are primary raw material, and the nutrients such as carbohydrate and amino acid are added, according to special ratios science
The effective component of proportion, each raw material cooperates, and provides beneficial supplement feed for the smooth safe overwintering of honeybee；
Gilliamella bacterium, glycerol, yeast powder are conducive to the injury that honeybee resists low temperature to itself, improve the honeybee of Wintering Period
Survival rate.Meanwhile honeybee eats the cold-resistant biological bacteria preparation provided in the embodiment of the present invention, can also enhance wintering bees constitution,
The disease incidence for reducing bee colony diarrhea, promotes honeybee health overwintering to promote the quick rejuvenation of bee colony next spring.
The embodiment of the present invention also provides a kind of preparation method of above-mentioned bee raising cold-resistant biological bacteria preparation, including following
Step:.
Gilliamella bacterium, glycerol, yeast powder, carbohydrate, amino acid and water are stirred, sealed after high pressure sterilization, is made
Obtain bee raising cold-resistant biological bacteria preparation.
In some embodiments, autoclaved temperature is 120-200 DEG C, time 10-60min.
As it can be seen that the embodiment of the present invention also provides a kind of preparation method of above-mentioned bee raising cold-resistant biological bacteria preparation,
In, Gilliamella bacterium cultivate after separating to honeybee intestinal flora and obtain by laboratory.By Gilliamella bacterium,
Glycerol, yeast powder, carbohydrate, amino acid and each ingredient of water seal after high pressure sterilization according to bottling after formula addition, honey can be obtained
Bee cultivation cold-resistant biological bacteria preparation.The bee raising that the above method is prepared cold-resistant biological bacteria preparation does not contain miscellaneous bacteria, honey
The overwintering success rate that bee colony can be improved after powder feeding reduces bee colony loss, accelerates the rejuvenation speed of coming year bee colony.
The embodiment of the present invention also provides a kind of application of above-mentioned bee raising cold-resistant biological bacteria preparation, by cold-resistant biological bacteria
Preparation makes an addition in the overwintering feed of honeybee.
In some embodiments, additive amount when addition are as follows: be 100ml preparation/overwintering feeding of 3-5kg for yellow honeybee kind
Material is 100ml preparation/8-10kg overwintering feed for black honeybee kind.
The embodiment of the present invention also provides a kind of application of bee raising cold-resistant biological bacteria preparation, honeybee forage of the invention
Using simple and convenient, through scientific and reasonable feeding feed stuff, bee colony can be made gradually to grow；Feed Energy provides sufficient sugar for honeybee
With nutrient, honeybee health is promoted to develop, reduces disease incidence, improve the quality and edible safety of honey.
Feature and performance of the invention are described in further detail with reference to embodiments.
A kind of bee raising cold-resistant biological bacteria preparation, following table 1 are formula table in 1-6 of the embodiment of the present invention:
Table 1- formula table
Using apis mellifera as gathering honey sample, by the cold-resistant biological bacteria preparation of the bee raising in the above embodiments 1-6
It is added in the overwintering feed of honeybee, compares apis mellifera bee colony living individuals ratio, feeding effect after overwintering
Such as following table 2:
Table 2- feeding effect:
As can be seen from Table 2: also there is certain resist for apis mellifera group, after replacing glucose with sucrose, fructose
Cold protecting effect, but its effect is inferior to glucose.After replacing glucose with alanine and arginine, the effect of cold-resistant biological bacteria preparation
Fruit is also by the weakening of part, its protecting effect is optimal after proline, alanine and arginine are added simultaneously, but it is compared with proline
The effect of addition group is not significant.
On the basis of the feeding effect of the above table 2, source of the glucose as nutritional ingredient is selected, proline is as ammonia
The source of base acid obtains formula table 3 below:
Table 3- formula table
Using apis mellifera and Apis mellifera mellifera as honeybee sample, by the bee raising cold-resistant in the above embodiments 7-12
In the overwintering feed of apis mellifera and Apis mellifera mellifera, and as a comparison with comparative example 1 and 2, comparative example 2 is biological bacteria preparation
With blank group, feeding effect such as following table 4:
Table 4- feeding effect:
By above table 4 it can be seen that adding in conjunction with yellow honeybee kind (apis mellifera) and black honeybee kind (Apis mellifera mellifera)
Add effect, which the overwintering success rate of bee colony individual 9-18% can be improved with cold-resistant biological bacteria preparation.By comparing not
Adding proportion with formula can find that the formula in embodiment 9,10,11 is best, in conjunction with cost of material, therefore selection example
Formula in 10 is as optimal additive capacity.
It can be seen that the feeding effect more than passing through is it can be seen that by comparing yellow honeybee kind (apis mellifera) and black
Cold-resistant biological bacteria preparation in the different embodiments of color honeybee kind (Apis mellifera mellifera) feeding finds that honeybee provided in an embodiment of the present invention is supported
Growing has certain freeze proof protective effect with cold-resistant biological bacteria preparation, using Gilliamella bacterium, glycerol and yeast powder as core
The heart is added carbohydrate and aminoacid ingredient, especially has using the freezeproof protectant of glucose and proline as honeybee small molecule
Very excellent effect can significantly improve the survival rate of Wintering Period feeding honeybee.
Embodiment 13
A kind of preparation method of bee raising cold-resistant biological bacteria preparation, comprising the following steps:
By -6 parts of 1-8 parts of Gilliamella bacterium, 1-6 parts of glycerol, 1-10 parts of yeast powder, 1-10 parts of carbohydrate and amino acid 1 with
And 80-100 parts of water mixing, wherein carbohydrate includes at least one in glucose, sucrose, fructose, maltose and soluble starch
Kind, amino acid includes proline, alanine, serine, tyrosine, arginine, lysine, threonine, valine and paddy ammonia
At least one of acid.
Temperature is 120-200 DEG C, stirs 10-60min, seals after high pressure sterilization, bee raising cold-resistant biological bacteria is made
Preparation.
Embodiment 14
A kind of application of bee raising cold-resistant biological bacteria preparation:
Use the time: the overwintering preceding preparatory stage, preparing bee colony overwintering feed is advisable.
Using: it is directly poured into overwintering feed after cold-resistant biological bacteria preparation is opened.
Adding proportion: for yellow honeybee kind: 1 bottle of cold-resistant biological bacteria preparation being added in 5-6kg overwintering feed, for black bee
Kind: 1 bottle of cold-resistant biological bacteria preparation is added in 8-10kg overwintering feed.
To sum up, the embodiment of the present invention provides a kind of bee raising cold-resistant biological bacteria preparation, preparation method and applications.It should
Bee raising cold-resistant biological bacteria preparation includes 1-8 parts of Gilliamella bacterium, 1-6 parts of glycerol, 1-10 parts of yeast powder and water
80-100 parts.The bee raising of embodiment of the present invention cold-resistant biological bacteria preparation includes honeybee cold-resistant advantage mushroom and other nutrition
Ingredient, at low cost, cost performance is high, honeybee is easy to absorb and the honeybee resistance of utilization, feeding is strong, and breeding potential is strong, especially gets over
The honeybee nursing death rate of teletostage is low, and simple production process is reasonable, is easy to large-scale industrial production, is readily transported, extensively
As honeybee overwintering feed, with high economic benefits and social benefits.
Embodiments described above is a part of the embodiment of the present invention, instead of all the embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Claims (10)
1. a kind of bee raising cold-resistant biological bacteria preparation, which is characterized in that comprise the following components in parts by weight:
1-8 parts of Gilliamella bacterium, 1-6 parts of glycerol, 1-10 parts of yeast powder and 80-100 parts of water.
2. bee raising according to claim 1 cold-resistant biological bacteria preparation, which is characterized in that including following parts by weight
Component: 2-5 parts of Gilliamella bacterium, 1-3 parts of glycerol, 1-3 parts of yeast powder and 90-100 parts of water.
3. bee raising according to claim 1 or 2 cold-resistant biological bacteria preparation, which is characterized in that described
The concentration of Gilliamella bacterium is 3 × 108/ part.
4. bee raising according to claim 1 cold-resistant biological bacteria preparation, which is characterized in that it further include nutriment,
The nutriment includes 1-10 parts and -6 parts of amino acid 1 of carbohydrate.
5. bee raising according to claim 4 cold-resistant biological bacteria preparation, which is characterized in that the carbohydrate includes grape
At least one of sugar, sucrose, fructose, maltose and soluble starch, preferably glucose.
6. bee raising according to claim 4 cold-resistant biological bacteria preparation, which is characterized in that the amino acid includes dried meat
At least one of propylhomoserin, alanine, serine, tyrosine, arginine, lysine, threonine, valine and glutamic acid,
Preferably proline.
7. a kind of preparation method of any one of -6 bee raisings with cold-resistant biological bacteria preparation according to claim 1, feature
It is, comprising the following steps:
The Gilliamella bacterium, the glycerol, the yeast powder, the carbohydrate, the amino acid and the water are stirred
It mixes, is sealed after high pressure sterilization, the bee raising cold-resistant biological bacteria preparation is made.
8. preparation method according to claim 7, which is characterized in that the autoclaved temperature is 120-200 DEG C, when
Between be 10-60min.
9. a kind of application of any one of -8 bee raising cold-resistant biological bacteria preparations according to claim 1, which is characterized in that
The cold-resistant biological bacteria preparation is made an addition in the overwintering feed of honeybee.
10. application according to claim 9, which is characterized in that the additive amount when addition are as follows: be for yellow honeybee kind
100ml preparation/3-5kg overwintering feed is 100ml preparation/8-10kg overwintering feed for black honeybee kind.
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| RO130726A2 (en) * | 2014-06-19 | 2015-12-30 | Melispec Production S.R.L. | Nutritional supplements with prophylactic effect for bees and preparation thereof |
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| CN108419757A (en) * | 2018-01-29 | 2018-08-21 | 东兰县百花园原生态养蜂场 | A kind of management method that honeybee is overwintering |
| US20180235191A1 (en) * | 2015-08-20 | 2018-08-23 | Api Holdings, Llc | Formulations and methods for promoting honeybee health |
| CN109055268A (en) * | 2018-08-21 | 2018-12-21 | 云南农业大学 | A kind of compound micro-ecological preparation and its application during bee raising |
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2019
- 2019-01-04 CN CN201910009691.4A patent/CN109601779A/en active Pending
Patent Citations (5)
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|---|---|---|---|---|
| RO130726A2 (en) * | 2014-06-19 | 2015-12-30 | Melispec Production S.R.L. | Nutritional supplements with prophylactic effect for bees and preparation thereof |
| US20180235191A1 (en) * | 2015-08-20 | 2018-08-23 | Api Holdings, Llc | Formulations and methods for promoting honeybee health |
| CN106962696A (en) * | 2017-05-12 | 2017-07-21 | 黄平县海松生态蜂业有限责任公司 | The method that honeybee forage and preparation method thereof and 1 March feed honeybee |
| CN108419757A (en) * | 2018-01-29 | 2018-08-21 | 东兰县百花园原生态养蜂场 | A kind of management method that honeybee is overwintering |
| CN109055268A (en) * | 2018-08-21 | 2018-12-21 | 云南农业大学 | A kind of compound micro-ecological preparation and its application during bee raising |
Non-Patent Citations (3)
| Title |
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| 刘春蕾等: "蜜蜂及熊蜂的肠道菌群种类和作用", 《中国蜂业》 * |
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Application publication date: 20190412 |