CN109593685A - A kind of anaerobic ammonoxidation bacterium concentrating method and device based on treated basalt fiber filler - Google Patents
A kind of anaerobic ammonoxidation bacterium concentrating method and device based on treated basalt fiber filler Download PDFInfo
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- CN109593685A CN109593685A CN201910028654.8A CN201910028654A CN109593685A CN 109593685 A CN109593685 A CN 109593685A CN 201910028654 A CN201910028654 A CN 201910028654A CN 109593685 A CN109593685 A CN 109593685A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a kind of anaerobic ammonoxidation bacterium concentrating method and devices based on treated basalt fiber filler, belong to the technical field of Sewage Biological Treatment, its drip irrigation device is as follows, the following steps are included: S1. activation system, it adds after nitrifying sludge and anaerobic ammonium oxidation sludge are mixed into the reactor for having been filled with treated basalt fiber, is sufficiently mixed sludge；Matrix raw water is sent into the reactor by S2. Initial stage of culture；Recirculated water is opened simultaneously, is circulated；S3. logarithmic growth phase controls raw water matrix water intake velocity using program setting, carries out the continuous fast enriching culture of anaerobic ammonia oxidizing bacteria；The enrichment method of anaerobic ammonia oxidizing bacteria of the present invention, can effectively shorten the anaerobic ammonia oxidizing bacteria generation cycle, and high activity proliferation is realized in the short time, carries out the enrichment culture of efficient activity anaerobic ammonia oxidizing bacteria, is laid the foundation for the engineer application of new biological nitrogen removal technology.
Description
Technical field
It is the present invention relates to the technical field of Sewage Biological Treatment, in particular to a kind of based on treated basalt fiber filler
Anaerobic ammonoxidation bacterium concentrating method.The enrichment method of anaerobic ammonia oxidizing bacteria of the present invention, can effectively shorten Anammox
Bacterium generation cycle, short time interior realization high activity proliferation carry out the enrichment culture of efficient activity anaerobic ammonia oxidizing bacteria, are new bio
The engineer application of denitrogenation technology lays the foundation.
Background technique
The key of Anammox denitrogenation technology is the enrichment culture of anaerobic ammonia oxidizing bacteria (Anammox bacterium).Currently, not yet
There is the relevant report that any authoritative institution carries out successfully purification & isolation to Anammox bacterium, ATCC(American Type Tissue Culture
Center) to NBRC(NITE Biological Resource Center) strain library also kept records of without related.In recent years, domestic outer portion scientific research
Team emerges one after another for the basic research of Anammox bacterium, generally using molecular biology method to (especially dirty useless in environment
In the activated sludge of water treatment technology) existing for Anammox bacterium carried out a large amount of experiment discussion.However, laboratory research or
The bottleneck problem occurred during small-scale engineering Application Research is then the fast enriching method and process stabilizing fortune of Anammox bacterium
Row means.Wherein, the main reason for enrichment of Anammox bacterium is difficult is that the Anammox bacterium generation cycle is long (generation time > 11 days),
Reproduction speed is slow, and the enrichment culture time is up to 1 year or more.For the anaerobe culture that such growth rate is slow, in order to anti-
Only flora is lost, general to carry out enriching floras by the way of the culture of gap.And existing gap training method is still generally existing
Anammox bacterium growth rate is slow, thallus yield is low, incubation time is long, the cell concentration of culture bacterium solution is low, flora is proliferated sump volume
The problems such as big.
Summary of the invention
The first purpose of the invention is to provide the present invention to propose a kind of anaerobism ammonia based on treated basalt fiber filler
Oxidation bacteria enrichment method effectively solves most of bottleneck problems existing in the prior art, has Anammox bacterium growth rate
Fastly, thallus high income, incubation time are short, cultivate the advantages that cell concentration of bacterium solution is high；Second object of the present invention is to propose
A kind of anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber filler effectively reduces while having effect same
Flora is proliferated sump volume.
Above-mentioned technical purpose of the invention has the technical scheme that
A kind of anaerobic ammonoxidation bacterium concentrating method based on treated basalt fiber filler, comprising the following steps:
S1. activation system will place 1 ~ 2 week nitrifying sludge and anaerobic ammonium oxidation sludge (300 ~ 600) in mass ratio: 1 mixing
It adds afterwards into the reactor for having been filled with treated basalt fiber, the sludge concentration of Initial stage of culture is made to reach 3000 ~ 6000 mg/
L, closed reactor, nitrogen are aerated 5 ~ 15 min, are sufficiently mixed sludge；
Matrix raw water is sent into the reactor by S2. Initial stage of culture；Recirculated water is opened simultaneously, is circulated, recirculated water is kept
36 ~ 39 DEG C of temperature；Hydraulic detention time is 10 ~ 30 h, with 3 ~ 7 days for a period, 5 ~ 15 min of intermittent aeration；It detects
Water-based concentration, when substrate concentration≤1/2 water inlet substrate concentration, into anaerobic ammonia oxidizing bacteria logarithmic growth phase；
S3. logarithmic growth phase controls raw water matrix water intake velocity using program setting, carries out the continuous quick of anaerobic ammonia oxidizing bacteria
Enrichment culture；
The principle formula of program setting is M=A × exp(K × T)；Wherein M is matrix charging rate, and A is Initial stage of culture setting
Substrate concentration, K are that matrix feeds constant, and T is that anaerobic ammonia oxidizing bacteria enters the cultivated days after logarithmic growth phase；Matrix includes ammonia
Nitrogen and nitrite nitrogen, the substrate concentration A range are 30 ~ 50 mg/L, and it is 0.08 ~ 0.12 that matrix, which feeds constant K range,.
Further, the method for modifying that treated basalt fiber filler uses for physics cladding process chitosan coat it is modified or
It is arbitrary a kind of in diethylin graft-modification method.
Further, the diameter of treated basalt fiber is 10 ~ 20 cm, and applicable pH range is 5 ~ 9, and surface is positively charged
Range is 2 ~ 20 mv.
Further, the filling rate range of treated basalt fiber filler is 30 ~ 70%.
Further, a kind of anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber, including raw water box 1, detest
Anaerobic ammonium oxidation reactor 2, sedimentation basin 3, PLC system 4, intake pump 6；The raw water box 1, anaerobic ammonia oxidation reactor 2, sedimentation basin
3 are sequentially connected with；Wherein raw water box 1 and anaerobic ammonia oxidation reactor 2 are connect by intake pump 6, the control switch of intake pump 6 with
The signal output end of PLC system 4 connects, for controlling to adjust the flow of inlet water of anaerobic ammonia oxidation reactor 2；Anammox is anti-
Answer device 2 in closed cylindrical, internal filling-modified basalt fibre；Mud discharging mouth 24 is arranged in 2 bottom of anaerobic ammonia oxidation reactor,
For the mud of anaerobic ammonia oxidation reactor 2 to be discharged；Water-bath thermal insulation clamp jacket layer 25 is arranged in 2 outer layer of anaerobic ammonia oxidation reactor；Water
It bathes 25 lower end of thermal insulation clamp jacket layer and circulating water intake 26 is set, circulating water outlet 27, the circulation for water is arranged in upper end；Anaerobism ammonia oxygen
Change bottom in reactor 2 and be laid with nitrogen aerated conduit 28, is aerated for nitrogen.
Further, anaerobic ammonia oxidation reactor 2 is provided with anaerobic ammonia oxidation reactor water inlet 21 and Anammox
Reactor water outlet 22；Raw water box 1 is connected with anaerobic ammonia oxidation reactor water inlet 21, for detesting the water importing of raw water box
In anaerobic ammonium oxidation reactor 2；Anaerobic ammonia oxidation reactor water outlet 22 is connect through U-shaped pipe with sedimentation basin 3, is used for oxygen ammonia oxygen
Change the water being discharged in reactor 2 and imports sedimentation basin 3.
Further, 2 top of anaerobic ammonia oxidation reactor is provided with gas outlet 23, and gas outlet 23 passes through gas flowmeter
211 are connected with fluid-tight water tank 5, for closing anaerobic ammonia oxidation reactor 2.
Further, the top setting of anaerobic ammonia oxidation reactor 2 is fluted, and fibre bundle supporting element 210 is arranged on groove,
Hang treated basalt fiber along water (flow) direction.
Further, 25 material of water-bath thermal insulation clamp jacket layer is any one in organic glass, carbon steel or glass reinforced plastic.
Further, the material of fibre bundle supporting element 210 is any one in stainless steel, organic glass, PP or PVC.
Further, the fixed form of fibre bundle supporting element 210 is that screw is fixed, is in adhesive type or welded type any one
Kind.
Further, the material of raw water box 1, anaerobic ammonia oxidation reactor 2 and sedimentation basin 3 be all organic glass, carbon steel or
Any one in glass reinforced plastic.
In conclusion the invention has the following advantages:
1. compared with existing Anammox bacterium culture technique, the Anammox bacterium generation cycle of this method culture is short (to be may be implemented
1.8 days generation cycles), high activity proliferation can be achieved in the short time, moreover it is possible to save matrix inlet amount, carry out efficient activity culture.
2. the modification basalt fibers used are compared with conventional fillers, flexibility, waterpower dispersibility, bioaffinity
It is more excellent, it is stronger to the adsorption capacity of Anammox thallus.The three-D space structure that filler disperses to present in water can promote
The high concentration of Anammox thallus is enriched with, and loss not easily to fall off.Filler itself is environmentally protective, and having used directly to fill, and is not necessarily to
Cost is saved in secondary treatment.
3.Anammox bacterium enriching apparatus is simple, need to only set water inlet substrate concentration, implements continuous and automatic control culture,
Without complex operations, that is, it can reach the purpose of fast enriching Anammox bacterium.
Detailed description of the invention
Fig. 1 is the anaerobic ammonoxidation bacterium concentrating device schematic diagram based on MBF new packing.
Appended drawing reference
1 raw water box, 2 anaerobic ammonia oxidation reactors, 3 sedimentation basins, 4PLC system, 5 fluid-tight water tanks, 6 intake pumps, 21 Anammoxs
Reactor water inlet, 22 anaerobic ammonia oxidation reactor water outlets, 23 air inlets, 24 mud discharging mouths, 25 water-bath thermal insulation clamp jacket layers, 26 follow
Ring water inlet, 27 circulating outlets, 28 nitrogen aerated conduits, 210 fibre bundle supporting elements, 211 gas flowmeters.
Specific embodiment
Embodiment
As shown in Figure 1, the anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber, including raw water box 1, anaerobism ammonia
Oxidation reactor 2, sedimentation basin 3, material are organic glass；Raw water box 1 is intake by intake pump 6 and anaerobic ammonia oxidation reactor
Mouth 21 is connected, and anaerobic ammonia oxidation reactor water outlet 22 is connect through U-shaped pipe with sedimentation basin 3；The control switch of intake pump 6 with
The signal output end of PLC system 4 connects, and controls to adjust flow of inlet water；Anaerobic ammonia oxidation reactor 2 is in closed cylindrical, top
End setting gas outlet 23 is connected by gas flowmeter 211 with fluid-tight water tank 5, and mud discharging mouth 24 is arranged in bottom, and water-bath is arranged in outer layer
Thermal insulation clamp jacket layer 25, using organic glass material, circulating water intake 26 is arranged in chuck layer lower end, and circulating water outlet is arranged in upper end
27；Bottom is laid with nitrogen aerated conduit 28 in anaerobic ammonia oxidation reactor 2, hangs treated basalt fiber, top along water (flow) direction
Hold groove that fibre bundle supporting element 210 is set.
Wherein, the program setting principle formula of PLC system 4 is M=A × exp(K × T)；Wherein M is matrix charging rate, A
For the substrate concentration of Initial stage of culture setting, K is that matrix feeds constant, and T is that anaerobic ammonia oxidizing bacteria enters the training after logarithmic growth phase
Support number of days；Matrix includes ammonia nitrogen, nitrite nitrogen, and the ammonia nitrogen of Initial stage of culture and the concentration of nitrite nitrogen are 30 ~ 35 mg/L, base
It is 0.1 that matter, which feeds constant K,.
Filling-modified basalt fibre is that basalt fibre is applied through physics cladding process chitosan in anaerobic ammonia oxidation reactor
Layer is modified (first to be etched basalt fibre surface with lye, is washed till neutrality, drying activation using deionized water；Object is used again
Reason coating, using chitosan as modifying agent, the basalt fibre dried after etching is immersed in chitosan solution, and hydrophily is made
Polymer modification basalt fibre) after, machinery is worked out and is obtained, and fiber bundle diameters are 15 cm, and applicable pH range is 5 ~ 9, surface
Positively charged about 5.5 mv, are fixed, media-filling rate about 35% using buckle-type.
210 material of packing support part on Anammox top is organic glass, adopts and is screwed.
Anaerobic ammonoxidation bacterium concentrating method based on treated basalt fiber specifically includes the following steps:
S1. activation system is thrown after mixing the nitrifying sludge and a small amount of anaerobic ammonium oxidation sludge 500:1 in mass ratio of placing 2 weeks
It adds to and has been filled in the anaerobic ammonia oxidation reactor 2 of treated basalt fiber filler, make Initial stage of culture anaerobic ammonia oxidation reactor 2
Interior sludge concentration reaches 5000 mg/L, closes anaerobic ammonia oxidation reactor 2, is aerated about by the nitrogen aerated conduit 28 of bottom
10 min, are sufficiently mixed sludge；
The matrix raw water that preparation is simulated in raw water box 1 is continuously pumped into Anammox reaction by intake pump 6 by S2. Initial stage of culture
In device 2, starts culture and maintain 24 h of hydraulic detention time；The recirculated water opened in water-bath insulation jacket 25 simultaneously circulates,
It is kept for about 39 DEG C of circulating water temperature, and opens the gas flowmeter 211 of the connection of gas outlet 23；With 3 ~ 5 days for a period, interval
Formula passes through 10 min of micro- aeration of nitrogen aerated conduit 28 of bottom, accelerates absorption of the treated basalt fiber to activated sludge；Prison
Measure water ammonia nitrogen and nitrite nitrogen concentration, when ammonia nitrogen and nitrite nitrogen concentration are below 15 mg/L, judge the reactor into
Enter anaerobic ammonia oxidizing bacteria logarithmic growth (proliferation) phase.
S3. logarithmic growth phase carries out anaerobism using the linkage intake pump intelligent control raw water matrix water intake velocity of PLC system 4
The continuous fast enriching culture of ammonia oxidation bacteria.
Long run test effect:
It raises and train 10 days, a large amount of activated sludge are carried on basalt fibre (MBF) filler, are in yellowish-brown, are started in reactor
Now apparent ascending gas bubbles are discharged about 22 mg/L of ammonia nitrogen concentration, 20 mg/L of nitrite nitrogen concentration.After 23 days, Anammox
Occurs a small amount of red in reactor 2 on MBF filler among filemot sludge, number of bubbles increases, and is discharged ammonia nitrogen concentration about 15
Mg/L, 13 mg/L of nitrite nitrogen concentration, nitrogen removal rate about 45%, volume nitrogen load reaches 0.04 kg/m3D.It raises and train 40 days
Or so, sludge color illustrates Anammox cell concentration liter between yellowish-brown and bronzing in anaerobic ammonia oxidation reactor 2
Height, proliferation trend are good.Culture 65 days or so, anaerobic ammonia oxidation reactor 2 is interior to there is macroscopic red floc sludge, is
System reaches 92% to the removal rate of ammonia nitrogen, reaches 95% to the removal rate of nitrite nitrogen, nitrogen removal rate is greater than 78%.
In conclusion a kind of anaerobic ammonoxidation bacterium concentrating method based on treated basalt fiber filler provided by the invention
And device, with Anammox bacterium growth rate, fast, thallus high income, incubation time are short, the cell concentration of culture bacterium solution is high, bacterium
The advantages that group's proliferation sump volume is small, has industrial value.
This specific embodiment is only explanation of the invention, is not limitation of the present invention, those skilled in the art
Member can according to need the modification that not creative contribution is made to the present embodiment after reading this specification, but as long as at this
All by the protection of Patent Law in the scope of the claims of invention.
Claims (10)
1. a kind of anaerobic ammonoxidation bacterium concentrating method based on treated basalt fiber filler, which is characterized in that including following step
It is rapid:
S1. activation system will place 1 ~ 2 week nitrifying sludge and anaerobic ammonium oxidation sludge (300 ~ 600) in mass ratio: 1 mixing
It adds afterwards into the reactor for having been filled with treated basalt fiber, the sludge concentration of Initial stage of culture is made to reach 3000 ~ 6000 mg/
L, closes the reactor, and nitrogen is aerated 5 ~ 15 min, is sufficiently mixed sludge；
Matrix raw water is sent into the reactor by S2. Initial stage of culture；Recirculated water is opened simultaneously, is circulated, recirculated water is kept
36 ~ 39 DEG C of temperature；Hydraulic detention time is 10 ~ 30 h, with 3 ~ 7 days for a period, 5 ~ 15 min of intermittent aeration；It detects
Water-based concentration, when substrate concentration≤1/2 water inlet substrate concentration, into anaerobic ammonia oxidizing bacteria logarithmic growth phase；
S3. logarithmic growth phase controls raw water matrix water intake velocity using program setting, carries out the continuous quick of anaerobic ammonia oxidizing bacteria
Enrichment culture；
The principle formula that described program is set is M=A × exp(K × T)；Wherein M is matrix charging rate, and A sets for Initial stage of culture
Fixed substrate concentration, K are that matrix feeds constant, and T is that anaerobic ammonia oxidizing bacteria enters the cultivated days after logarithmic growth phase；The base
Matter includes ammonia nitrogen and nitrite nitrogen, and the substrate concentration A range is 30 ~ 50 mg/L, and the matrix charging constant K range is
0.08~0.12。
2. a kind of anaerobic ammonoxidation bacterium concentrating method based on treated basalt fiber filler according to claim 1,
It is characterized in that, the treated basalt fiber is using the modification of physics cladding process chitosan coat or diethylin graft-modification method
In it is arbitrary a kind of.
3. a kind of anaerobic ammonoxidation bacterium concentrating method based on treated basalt fiber filler according to claim 1,
It is characterized in that, it is 5 ~ 9 using pH range that the diameter of the treated basalt fiber, which is 10 ~ 20 cm, the positively charged range in surface
For 2 ~ 20 mv.
4. the according to claim a kind of anaerobic ammonoxidation bacterium concentrating method based on treated basalt fiber filler, special
Sign is that the filling rate range of the treated basalt fiber is 30 ~ 70%.
5. a kind of anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber filler, which is characterized in that including raw water box
(1), anaerobic ammonia oxidation reactor (2), sedimentation basin (3), PLC system (4), intake pump (6)；The raw water box (1), anaerobism ammonia oxygen
Change reactor (2), sedimentation basin (3) is sequentially connected with；Wherein the raw water box (1) passes through with the anaerobic ammonia oxidation reactor (2)
Intake pump (6) connection, the control switch of the intake pump (6) connect with the signal output end of the PLC system (4), use
In the flow of inlet water for controlling to adjust the anaerobic ammonia oxidation reactor (2)；The anaerobic ammonia oxidation reactor (2) is in closed circle
Cylindricality, internal filling-modified basalt fibre；Mud discharging mouth (24) are arranged in anaerobic ammonia oxidation reactor (2) bottom, for arranging
The mud of the anaerobic ammonia oxidation reactor (2) out；Water-bath thermal insulation clamp jacket layer is arranged in anaerobic ammonia oxidation reactor (2) outer layer
(25), the water-bath thermal insulation clamp jacket layer (25) lower end setting circulating water intake (26), upper end are arranged circulating water outlet (27), are used for
The circulation of water；The interior bottom of anaerobic ammonia oxidation reactor (2) is laid with nitrogen aerated conduit (28), is aerated for nitrogen.
6. a kind of anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber filler according to claim 5,
It is characterized in that, the anaerobic ammonia oxidation reactor (2) is provided with anaerobic ammonia oxidation reactor water inlet (21) and Anammox
Reactor water outlet (22)；The raw water box (1) is connected with the anaerobic ammonia oxidation reactor water inlet (21), is used for institute
The water for stating raw water box imports in the anaerobic ammonia oxidation reactor (2)；The anaerobic ammonia oxidation reactor water outlet (22) is through U-shaped
Pipeline is connect with the sedimentation basin (3), and the water for will be discharged in the anaerobic ammonium oxidation reactor (2) imports the sedimentation basin
(3).
7. a kind of anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber filler according to claim 5,
It is characterized in that, anaerobic ammonia oxidation reactor (2) top is provided with gas outlet (23), and the gas outlet (23) passes through gas stream
Meter (211) is connected with fluid-tight water tank (5), for closing the anaerobic ammonia oxidation reactor (2).
8. a kind of anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber filler according to claim 5,
It is characterized in that, the top setting of the anaerobic ammonia oxidation reactor (2) is fluted, and fibre bundle supporting element is arranged on the groove
(210), the treated basalt fiber is hung along water (flow) direction.
9. a kind of anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber filler according to claim 5,
It is characterized in that, the fixed form of the fibre bundle supporting element (210) is that screw is fixed, is in adhesive type or welded type any one
Kind.
10. a kind of anaerobic ammonoxidation bacterium concentrating device based on treated basalt fiber filler according to claim 5,
It is characterized in that, the material of the raw water box (1), anaerobic ammonia oxidation reactor (2) and sedimentation basin (3) is all organic glass, carbon steel
Or any one in glass reinforced plastic.
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