CN107523474A - The culture apparatus and cultural method of a kind of anaerobic ammonia oxidizing bacteria - Google Patents
The culture apparatus and cultural method of a kind of anaerobic ammonia oxidizing bacteria Download PDFInfo
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- CN107523474A CN107523474A CN201710993942.8A CN201710993942A CN107523474A CN 107523474 A CN107523474 A CN 107523474A CN 201710993942 A CN201710993942 A CN 201710993942A CN 107523474 A CN107523474 A CN 107523474A
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- oxidizing bacteria
- anaerobic ammonia
- ammonia oxidizing
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- 241001453382 Nitrosomonadales Species 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 51
- 239000010802 sludge Substances 0.000 claims abstract description 24
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 32
- 238000009413 insulation Methods 0.000 claims description 30
- 239000002351 wastewater Substances 0.000 claims description 19
- 229910052757 nitrogen Inorganic materials 0.000 claims description 16
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 10
- 239000008236 heating water Substances 0.000 claims description 9
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims description 5
- 229910021529 ammonia Inorganic materials 0.000 claims description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 239000011574 phosphorus Substances 0.000 claims description 4
- 239000011162 core material Substances 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 3
- 230000002459 sustained effect Effects 0.000 claims description 3
- 239000000945 filler Substances 0.000 abstract description 2
- 239000007787 solid Substances 0.000 abstract description 2
- 239000000725 suspension Substances 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 10
- 230000003647 oxidation Effects 0.000 description 7
- 238000007254 oxidation reaction Methods 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000010865 sewage Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000001546 nitrifying effect Effects 0.000 description 3
- 238000010926 purge Methods 0.000 description 3
- PDNNQADNLPRFPG-UHFFFAOYSA-N N.[O] Chemical compound N.[O] PDNNQADNLPRFPG-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 229910018890 NaMoO4 Inorganic materials 0.000 description 1
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229910052927 chalcanthite Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of culture apparatus of anaerobic ammonia oxidizing bacteria, including biological culture reactor and the attemperator for being incubated to the biological culture reactor, wherein, biological culture reactor includes：Reactor body, the reactor body are the cylindrical chamber that both ends use flange seal, and the reactor body flange in the bottom connection water inlet, top flange connection exhaust outlet, the top of the reactor body offer delivery port；Non-woven carrier, it is filled in the inside of the reactor body.Compared with prior art, the present invention has following prominent characteristics：The preferable non-woven carrier of bioaffinity has been hung in reactor, and arranged in pencil, it is larger with the contact area of water, anaerobic ammonia oxidizing bacteria is easily adhered on filler, it is not easy as water body is lost in, both strain enrichment time had been substantially reduced, the solid suspension concentration of water outlet has been reduced again, effectively reduces sludge generating capacity.
Description
Technical field
The present invention relates to technical field of biological sewage treatment, and in particular to a kind of culture apparatus of anaerobic ammonia oxidizing bacteria and training
The method of supporting.
Background technology
Social economy's high speed development, environmental problem getting worse, the body eutrophication problem as caused by nitrogen, phosphorus excess are got over
More to protrude, the nitrogen of wastewater treatment in China, phosphorus discharge standard also improve constantly, the overall control to polluters such as nitrogen, phosphorus
Pay attention to day by day.Currently, the effluent of municipal sewage plant in China has to comply with the one-level A standards in town sewage discharge standard,
Water outlet total nitrogen is less than 15mg/L.
Biological denitrificaion is the method for current more mature and reliable, and the microorganism for being responsible for denitrogenation is mainly nitrifier and denitrification
Bacterium, in practical engineering application, the growing environment of two kinds of thalline is entirely different, therefore general all by nitrification and denitrification process point
Open, technique is more complicated；Secondly, nitrification needs a large amount of aerations and extra basicity, and denitrification process additionally adds organic carbon source, invests
It is higher with operating cost.Simultaneously as the biodegradability of most of actual sewage is relatively poor, and easily dropped in nitrifying process
Organic matter is solved by the degraded of heterotroph so that denitrification process causes the clearance of nitrogen to substantially reduce because of carbon source deficiency, water outlet
In contain substantial amounts of nitrate nitrogen.
Anaerobic ammonia oxidation process is a kind of new and effective bio-denitrification technology proposed by Holland technology university.In anaerobism bar
Under part, anaerobic ammonium oxidizing bacteria is nitrogen directly by ammonium oxidation using nitrite as electron acceptor.With traditional handicraft phase
Than anaerobic ammonia oxidation process, without adding organic carbon source, because anaerobic ammonia oxidizing bacteria generation time is grown, can subtract without oxygen supply
Few sludge yield, is a kind of cost-effective biological denitrificaion approach.
Although the country is existing for the experiment case study of the technique, anaerobic ammonia oxidizing bacteria breeding is slow in existing case
Slowly, acclimation period is grown, and culture apparatus is cumbersome.The activity for stilling need higher concentration in existing anaerobic ammonia oxidation process mostly is dirty
Mud coordinates the mode of stirring to run as inoculation carrier, while also needs the progress auxiliary such as mud-water separation or sludge reflux to set
It is standby, seriously limit effective popularization of the technique in Practical Project.Therefore, above-mentioned asked for the technique is existing at this stage
Topic, it is current highly important task to take simple effective method lifting process reliability of operation and economy.
The content of the invention
It is a primary object of the present invention to, the defects of overcoming existing culture apparatus to exist, and a kind of anaerobism ammonia oxygen is provided
Change the culture apparatus and cultural method of bacterium, thus more suitable for practicality, and with the value in industry.
The object of the invention to solve the technical problems is realized using following technical scheme.
A kind of culture apparatus of anaerobic ammonia oxidizing bacteria, including biological culture reactor and for give the biological culture reaction
The attemperator of device insulation, wherein, biological culture reactor includes：
Reactor body, the reactor body use the cylindrical chamber of flange seal, the reactor body for both ends
Flange in the bottom is provided with water inlet, top flange is provided with exhaust outlet, the top of the reactor body offers delivery port；
Non-woven carrier, it is filled in the inside of the reactor body.
As a kind of perferred technical scheme, the exhaust outlet connection inlet water tank, delivery port connection outer tank,
Form fluid-tight construction.
As a kind of perferred technical scheme, the attemperator is coated on the guarantor of the reactor body outer circumferential side
Warm chuck and the heating water tank that warm water is provided to the insulation jacket, wherein the insulation water inlet of the insulation jacket is arranged on
The bottom of the insulation jacket, insulation water out are arranged on the top of the insulation jacket.
As a kind of perferred technical scheme, the insulation water out is connected with the heating water tank, forms circulating water
System.
As a kind of perferred technical scheme, the non-woven carrier is strip, and the reactor body is fixed at both ends
Both ends.
As a kind of perferred technical scheme, the non-woven carrier list Shu Changdu is 40~50cm, and width is 1~5cm,
Thickness is 0.5~1cm, and the non-woven carrier is more, the filling rate inside the reactor body for 10%~
50%.
As a kind of perferred technical scheme, the non-woven carrier is vertical hanging or surround in the reactor body
The mode that core is fixed in portion is fixed on inside the reactor body.
As a kind of perferred technical scheme, the reactor body is circular cylindrical cavity, and the internal diameter of the cavity and
Effective depth is respectively 25cm, 65cm.
A kind of cultural method of the culture apparatus of anaerobic ammonia oxidizing bacteria, using anaerobism ammonia oxygen according to claim 1
The culture apparatus for changing bacterium carries out the culture of anaerobic ammonia oxidizing bacteria, comprises the following steps：
S1, open attemperator so that the reactor body internal temperature is maintained at 35-38 DEG C；
S2, it is passed through synthetic wastewater so that the non-woven carrier is all immersed in the synthetic wastewater；
S3, input seed sludge, the input amount of the seed sludge is 200-2000mg/L；
S4, sustained response 7-20d, obtain anaerobic ammonia oxidizing bacteria.
As a kind of perferred technical scheme, the ammonia nitrogen concentration of the synthetic wastewater is 35~350mg/L, and nitrite nitrogen is dense
It is 75~650mg/L to spend for 30~300mg/L, total nitrogen concentration.
As a kind of perferred technical scheme, the seed sludge is nitrifying sludge, anaerobic ammonium oxidation sludge and denitrification
The combination of one or both of sludge.
As a kind of perferred technical scheme, the dissolved oxygen concentration of the synthetic wastewater is less than 0.6mg/L.
Compared with prior art, the present invention has following prominent characteristics：
1st, the preferable non-woven carrier of bioaffinity has been hung in reactor, and has been arranged in pencil, the contact surface with water
Product is larger, and anaerobic ammonia oxidizing bacteria is easily adhered on filler, is not easy, as water body is lost in, both to have substantially reduced strain enrichment time,
The solid suspension concentration (SS concentration) of water outlet is reduced again, effectively reduces sludge generating capacity.Therefore, in strain enrichment process
In, the seed sludge concentration added in the present apparatus is low, without using any type of mixing apparatus, is only promoted by hydraulic action
The progress of Anammox reaction, while reach the purpose of enrichment anaerobic ammonia oxidizing bacteria and denitrogenation.
2nd, because the growing environment needed for anaerobic ammonia oxidizing bacteria is more harsh, therefore in the present invention by the way of nitrogen purging
Dissolved oxygen low concentration control is carried out for synthetic wastewater, sufficient growth and breeding can be provided for domestication early stage of anaerobic ammonia oxidizing bacteria
Environment, accelerate culture process.With the breeding of anaerobic ammonia oxidizing bacteria, caused nitrogen enters water feed apparatus, a side in reactor
Face forms fluid-tight construction, can effectively prevent air blow back from destroying reactor body anaerobic environment；On the other hand, caused nitrogen can
As the low concentration dissolved oxygen in effective purging gas shielded inlet water tank, formation recycles, and saves energy consumption.
Brief description of the drawings
Fig. 1 is the structural representation of the culture apparatus of anaerobic ammonia oxidizing bacteria in the present invention；
Wherein：1- reactor bodies, 11- water inlets, 12- exhaust outlets, 13- delivery ports, 2- non-woven carriers, 3- water inlets
Case, 4- outer tanks, 5- insulation jackets, 51- insulation water inlets, 52- insulation water outs, 6- heating water tanks.
Embodiment
Further to illustrate the present invention to reach the technological means and effect that predetermined goal of the invention is taken, to according to this
Culture apparatus and cultural method its embodiment, the feature and its effect of the anaerobic ammonia oxidizing bacteria proposed are invented, specifically
It is bright as after.
As shown in figure 1, the invention discloses a kind of culture apparatus of anaerobic ammonia oxidizing bacteria, including biological culture reactor and
For the attemperator being incubated to biological culture reactor, wherein, biological culture reactor includes：
Reactor body 1, reactor body 1 are the cylindrical chamber that both ends use flange seal, and both ends are carried out using flange
Sealing, can effectively maintain anaerobism or anaerobic environment, and cylindrical chamber is placed vertically, and the flange in the bottom of reactor body 1 is provided with
Water inlet 11, top flange are provided with exhaust outlet 12, the top of reactor body 1 offers delivery port 13, the conjunction of input in addition
Discharged into waste water from the entrance of the bottom of reactor body 1, caused nitrogen from the top of reactor body 1, in addition synthetic wastewater
The outflow reactor body 1 of delivery port 13 set after the completion of reaction from top.
Also include non-woven carrier 2, the inside of reactor body 1 is filled in, due to the bioaffinity of non-woven carrier 2
It is good, therefore the microorganism in water is easily adhered to growth and breeding on non-woven carrier 2, anaerobic ammonia oxidizing bacteria is enriched with.
Further, exhaust outlet 12 connects inlet water tank 3, delivery port 13 connects outer tank 4, is respectively formed fluid-tight construction.And
The nitrogen that exhaust outlet 12 is discharged, which is discharged into inlet water tank 3, to form circulation as the low concentration oxygen in effective purging gas shielded inlet water tank 3
Utilize, save energy consumption.
As a kind of perferred technical scheme, attemperator is coated on the insulation jacket 5 of the outer circumferential side of reactor body 1
And the heating water tank 6 of warm water is provided to insulation jacket 5, the insulation water inlet 51 of wherein insulation jacket 5 is arranged on insulation jacket 5
Bottom, insulation water out 52 is arranged on the top of insulation jacket 5, and is incubated water out 52 and is connected with heating water tank 6, and formation follows
Ring water system.In order to increase the power of this circulation reclaimed water, in addition to water pump, it is preferred that water pump is arranged on heating water tank 6
Water side.
Further, non-woven carrier 2 is strip, and both ends are fixed on the both ends of reactor body 1, are arranged in instead in vertical shape
Answer the inside of device body 1.
Further, 2 single Shu Changdu of non-woven carrier is 40~50cm, and width is 1~5cm, and thickness is 0.5~1cm, and
Non-woven carrier 2 is more, and the more vertical spacing of non-woven carrier 2 arrangements, the filling rate inside reactor body 1 is 10%
~50%, i.e. the volume of non-woven carrier 2 accounts for the 10-50% of dischargeable capacity in reactor body 1.
Further, non-woven carrier 2 is that vertical hanging or the mode around the inner fixed core material of reactor body 1 are fixed
Inside reactor body 1.
Further, reactor body 1 is circular cylindrical cavity, and the internal diameter of cavity and effective depth be respectively 25cm,
65cm。
The invention also discloses a kind of cultural method of the culture apparatus of anaerobic ammonia oxidizing bacteria, using anaerobism as described above
The culture apparatus of ammonia oxidation bacteria carries out the culture of anaerobic ammonia oxidizing bacteria, comprises the following steps：
S1, open attemperator so that the internal temperature of reactor body 1 is maintained at 35-38 DEG C；
S2, it is passed through synthetic wastewater so that non-woven carrier 2 is all immersed in synthetic wastewater；
S3, input seed sludge, the input amount of seed sludge is 200-2000mg/L；
S4, sustained response 7-20d, obtain anaerobic ammonia oxidizing bacteria.
Further, the ammonia nitrogen concentration of synthetic wastewater is 35~350mg/L, and nitrite nitrogen concentration is 30~300mg/L, always
Nitrogen concentration is 75~650mg/L.
Further, seed sludge is one or both of nitrifying sludge, anaerobic ammonium oxidation sludge and denitrification sludge
Combination.
Further, the dissolved oxygen concentration of synthetic wastewater is less than 0.6mg/L.
In order to further illustrate the present invention, with reference to training of the specific embodiment to anaerobic ammonia oxidizing bacteria provided by the invention
The culture apparatus and cultural method for supporting device are described in detail, but should not be construed as the limit to the scope of the present invention
It is fixed.
Synthetic wastewater (substrate concentration is shown in Table 1) is entered in reactor body 1 by water inlet 11, is spaced in reactor body 1
Arrangement is filled with non-woven carrier 2, is all immersed in synthetic wastewater, and puts into 1000mg/L seed sludge, wherein being inoculated with
Sludge is denitrification sludge.Synthetic wastewater and denitrification sludge converge after by hydraulic action along on the space of non-woven carrier 2
Rise, contacted with the surface of non-woven carrier 2, denitrification sludge attachment non-woven carrier 2 in part stops.Water after processing is by top
Delivery port 13 escapes and enter outer tank 4, and caused nitrogen escapes and enter inlet water tank 3 by the exhaust outlet 12 at top.Meanwhile
Circulating hot water in heating water tank 6 is entered by the insulation water inlet 51 of the bottom of insulation jacket 5, the overflow of insulation water out 52 on top
Go out to be circulated, it is 37 DEG C to ensure the interior reaction temperature scope of reactor body 1.
The synthetic wastewater water quality situation of table 1
| Matrix | Addition (mg/L) |
| NaNO2(NO2-N) | 30~300 |
| (NH4)2SO4(NH3-N) | 35~350 |
| NaHCO3 | 500 |
| KH2PO4 | 27 |
| MgSO4·7H2O | 300 |
| CaCl2·2H2O | 180 |
| FeSO4 | 5 |
| EDTA | 20 |
| ZnSO4·7H2O | 0.43 |
| CoCl2·6H2O | 0.24 |
| MnCl2·4H2O | 0.99 |
| CuSO4·5H2O | 0.25 |
| NaMoO4·2H2O | 0.22 |
| NiCl2·6H2O | 0.19 |
| H3BO4 | 0.014 |
The influent concentration of synthetic wastewater is slowly improved from initial low concentration, and the residence time is gradually shortened by 24h, reactor
Influent ammonium concentration is 35mg/L in body 1, and nitrite nitrogen concentration is 30mg/L, after culture apparatus is run 10 days, water outlet ammonia nitrogen
Concentration is 25mg/L, and nitrite nitrogen concentration is 20mg/L, thus speculates in reactor body 1 Anammox occurred
Bacterium.
The above described is only a preferred embodiment of the present invention, any formal limitation not is made to the present invention, though
So the present invention is disclosed above with preferred embodiment, but is not limited to the present invention, any to be familiar with this professional technology people
Member, without departing from the scope of the present invention, when the technology contents using the disclosure above make a little change or modification
For the equivalent embodiment of equivalent variations, as long as being the content without departing from technical solution of the present invention, the technical spirit according to the present invention
Any simple modification, equivalent change and modification made to above example, in the range of still falling within technical solution of the present invention.
Claims (10)
1. a kind of culture apparatus of anaerobic ammonia oxidizing bacteria, it is characterised in that including biological culture reactor and for the life
The attemperator of thing cultivation reactor insulation, wherein, biological culture reactor includes：
Reactor body（1）, the reactor body（1）The cylindrical chamber of flange seal, the reactor sheet are used for both ends
Body（1）Flange in the bottom is provided with water inlet（11）, top flange be provided with exhaust outlet（12）, the reactor body（1）It is upper
Portion offers delivery port（13）；
Non-woven carrier（2）, it is filled in the reactor body（1）Inside.
2. the culture apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, it is characterised in that the exhaust outlet（12）Connection
Inlet water tank（3）, the delivery port（13）Connect outer tank（4）, it is respectively formed fluid-tight construction.
3. the culture apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, it is characterised in that the attemperator includes bag
Overlay on the reactor body（1）The insulation jacket of outer circumferential side（5）And to the insulation jacket（5）The heating water of warm water is provided
Case（6）, wherein the insulation jacket（5）Insulation water inlet（51）It is arranged on the insulation jacket（5）Bottom, insulation water go out
Mouthful（52）It is arranged on the insulation jacket（5）Top.
4. the culture apparatus of anaerobic ammonia oxidizing bacteria according to claim 3, it is characterised in that the insulation water out（52）
With the heating water tank（6）Connection, form circulation.
5. the culture apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, it is characterised in that the non-woven carrier（2）
For strip, the reactor body is fixed at both ends（1）Both ends.
6. the culture apparatus of anaerobic ammonia oxidizing bacteria according to claim 1 or 5, it is characterised in that the non-woven carrier
（2）Single Shu Changdu is 40 ~ 50 cm, and width is 1 ~ 5 cm, and thickness is 0.5 ~ 1 cm, and the non-woven carrier（2）For more,
In the reactor body（1）Internal filling rate is 10% ~ 50%.
7. the culture apparatus of anaerobic ammonia oxidizing bacteria according to claim 1 or 5, it is characterised in that the non-woven carrier
（2）For vertical hanging or around the reactor body（1）The mode of inner fixed core material is fixed on the reactor body（1）
It is internal.
8. the culture apparatus of anaerobic ammonia oxidizing bacteria according to claim 1, it is characterised in that the reactor body（1）
For circular cylindrical cavity, and the internal diameter of the cavity and effective depth are respectively 25 cm, 65 cm.
9. a kind of cultural method of the culture apparatus of anaerobic ammonia oxidizing bacteria, it is characterised in that using according to claim 1
The culture apparatus of anaerobic ammonia oxidizing bacteria carries out the culture of anaerobic ammonia oxidizing bacteria, comprises the following steps：
S1, open attemperator so that the reactor body（1）Internal temperature is maintained at 35-38 DEG C；
S2, it is passed through synthetic wastewater so that the non-woven carrier（2）All it is immersed in the synthetic wastewater；
S3, input seed sludge, the input amount of the seed sludge is 200-2000mg/L；
S4, sustained response 7-20d, obtain anaerobic ammonia oxidizing bacteria.
10. the cultural method of anaerobic ammonia oxidizing bacteria according to claim 9, it is characterised in that the ammonia of the synthetic wastewater
Nitrogen concentration is 35 ~ 350 mg/L, and nitrite nitrogen concentration is 30 ~ 300 mg/L, and total nitrogen concentration is 75 ~ 650mg/L, and total phosphorus concentration is
6~50 mg/L。
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441419A (en) * | 2018-02-11 | 2018-08-24 | 中国电建集团华东勘测设计研究院有限公司 | Small-sized targeting strain intelligence culture apparatus and method |
| CN110526392A (en) * | 2019-09-18 | 2019-12-03 | 江苏大学 | Anaerobic ammonoxidation bacterium concentrating device and application method based on cushion basalt fibre filler |
| CN119286618A (en) * | 2024-12-16 | 2025-01-10 | 四川发展环境科学技术研究院有限公司 | Anaerobic ammonia oxidation strain culture device and culture method |
| CN119286618B (en) * | 2024-12-16 | 2025-02-25 | 四川发展环境科学技术研究院有限公司 | A culture device and a culture method for anaerobic ammonia-oxidizing bacteria |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101302059A (en) * | 2008-06-24 | 2008-11-12 | 大连理工大学 | Inverted denitrification process film bioreactor |
| CN101538087A (en) * | 2009-05-04 | 2009-09-23 | 大连理工大学 | CMABR (carbon tube membrane-aerated biofilm reactor) for running single-stage autotrophic biological nitrogen removal process |
| WO2013185350A1 (en) * | 2012-06-15 | 2013-12-19 | 乐金电子研发中心(上海)有限公司 | Internal-circulation aeration anammox-membrane bioreactor |
| CN203540294U (en) * | 2013-10-08 | 2014-04-16 | 安徽晋煤中能化工股份有限公司 | Low-pressure absorbing system for reducing consumption of urea ammonia |
| CN105347485A (en) * | 2015-11-12 | 2016-02-24 | 山东大学 | Bio-membrane reactor realizing synchronous nitrification, denitrification and anaerobic ammoxidation and fast realization method |
| CN105366673A (en) * | 2014-08-21 | 2016-03-02 | 苏州格瑞展泰再生能源有限公司 | Accurately controlled horizontal physical activation reactor and technology thereof |
| CN105668789A (en) * | 2016-01-28 | 2016-06-15 | 中国环境科学研究院 | Integrated denitrification sewage treatment device and sewage treatment method |
| CN106277315A (en) * | 2016-08-12 | 2017-01-04 | 江苏艾特克环境工程设计研究院有限公司 | A kind of denitrification microorganism nest based on basalt fibre filler and cultural method thereof |
| CN106517507A (en) * | 2016-12-19 | 2017-03-22 | 河北工业大学 | Method and device for quickly starting anaerobic ammonia oxidation at normal temperature |
| CN207452090U (en) * | 2017-10-23 | 2018-06-05 | 江苏艾特克环境工程设计研究院有限公司 | A kind of culture apparatus of anaerobic ammonia oxidizing bacteria |
-
2017
- 2017-10-23 CN CN201710993942.8A patent/CN107523474A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101302059A (en) * | 2008-06-24 | 2008-11-12 | 大连理工大学 | Inverted denitrification process film bioreactor |
| CN101538087A (en) * | 2009-05-04 | 2009-09-23 | 大连理工大学 | CMABR (carbon tube membrane-aerated biofilm reactor) for running single-stage autotrophic biological nitrogen removal process |
| WO2013185350A1 (en) * | 2012-06-15 | 2013-12-19 | 乐金电子研发中心(上海)有限公司 | Internal-circulation aeration anammox-membrane bioreactor |
| CN203540294U (en) * | 2013-10-08 | 2014-04-16 | 安徽晋煤中能化工股份有限公司 | Low-pressure absorbing system for reducing consumption of urea ammonia |
| CN105366673A (en) * | 2014-08-21 | 2016-03-02 | 苏州格瑞展泰再生能源有限公司 | Accurately controlled horizontal physical activation reactor and technology thereof |
| CN105347485A (en) * | 2015-11-12 | 2016-02-24 | 山东大学 | Bio-membrane reactor realizing synchronous nitrification, denitrification and anaerobic ammoxidation and fast realization method |
| CN105668789A (en) * | 2016-01-28 | 2016-06-15 | 中国环境科学研究院 | Integrated denitrification sewage treatment device and sewage treatment method |
| CN106277315A (en) * | 2016-08-12 | 2017-01-04 | 江苏艾特克环境工程设计研究院有限公司 | A kind of denitrification microorganism nest based on basalt fibre filler and cultural method thereof |
| CN106517507A (en) * | 2016-12-19 | 2017-03-22 | 河北工业大学 | Method and device for quickly starting anaerobic ammonia oxidation at normal temperature |
| CN207452090U (en) * | 2017-10-23 | 2018-06-05 | 江苏艾特克环境工程设计研究院有限公司 | A kind of culture apparatus of anaerobic ammonia oxidizing bacteria |
Non-Patent Citations (2)
| Title |
|---|
| 李祥;黄勇;袁怡;: "厌氧氨氧化菌活性恢复及富集培养研究", 环境污染与防治, vol. 32, no. 01, pages 61 - 66 * |
| 赫俊国等: "《城市水环境评价与技术》", 31 January 2008, 哈尔滨工业大学出版社, pages: 192 - 193 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441419A (en) * | 2018-02-11 | 2018-08-24 | 中国电建集团华东勘测设计研究院有限公司 | Small-sized targeting strain intelligence culture apparatus and method |
| CN110526392A (en) * | 2019-09-18 | 2019-12-03 | 江苏大学 | Anaerobic ammonoxidation bacterium concentrating device and application method based on cushion basalt fibre filler |
| CN119286618A (en) * | 2024-12-16 | 2025-01-10 | 四川发展环境科学技术研究院有限公司 | Anaerobic ammonia oxidation strain culture device and culture method |
| CN119286618B (en) * | 2024-12-16 | 2025-02-25 | 四川发展环境科学技术研究院有限公司 | A culture device and a culture method for anaerobic ammonia-oxidizing bacteria |
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