CN109589278A - The preparation process of high stable prinsepia utilis royle oil liposome and its application in cosmetics - Google Patents
The preparation process of high stable prinsepia utilis royle oil liposome and its application in cosmetics Download PDFInfo
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- CN109589278A CN109589278A CN201811175968.2A CN201811175968A CN109589278A CN 109589278 A CN109589278 A CN 109589278A CN 201811175968 A CN201811175968 A CN 201811175968A CN 109589278 A CN109589278 A CN 109589278A
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- prinsepia utilis
- utilis royle
- royle oil
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/002—Preparations for repairing the hair, e.g. hair cure
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Abstract
The invention discloses a kind of preparation processes of high stable prinsepia utilis royle oil liposome, prinsepia utilis royle oil liposome is prepared using high/low temperature alternation and high-pressure homogeneous combination process, prinsepia utilis royle oil can be solved the problems, such as in this way in stability, encapsulation rate, slow release effect and is conveniently added to the medium aspect of water, effectively increase the encapsulation rate of prinsepia utilis royle oil, encapsulation rate > 98%.Active matter prinsepia utilis royle oil loss through effectively coating is small, and stability is good.The organic solvent and additive of any toxic side effect is not used, can directly act on as green safe cosmetics.Prinsepia utilis royle oil liposome is nanoscale, improves bioavilability, can allow it to be widely used in the preparation of all kinds of cosmetics with water arbitrarily than dissolving each other.The invention also discloses application of the above-mentioned high stable prinsepia utilis royle oil liposome in cosmetics, skin can be made to moisten soft, and can treat pernio, also have fabulous opsonic action to hair maintenance.
Description
Technical field
The present invention relates to a kind of preparation process of high stable prinsepia utilis royle oil liposome and its applications in cosmetics, belong to
The technical field of carrier system in cosmetics technology of preparing.
Background technique
Green bur is a kind of flat mesh category shrub plant of perennial rosaceae, and scientific name is total flower Prinsepia utilis.Green bur it is edible,
Skin care, beauty and cmm of simple prescription etc. suffer from long history and extensive purposes.
Modern research shows that containing to the highly important three classes fatty acid of human health in prinsepia utilis royle oil, wherein saturated fat
Fat acid (palmitinic acid and stearic acid), monounsaturated fatty acids (oleic acid) and polyunsaturated fatty acid (linoleic acid plus linolenic acid) contain
The amount of having composition ratio is (0.6~0.7): 1:1, so the grease is relatively good natural nutrition edible blend oil, it is often edible
Blood lipid can be reduced, blood pressure is adjusted, promotes microcirculation, enhances the resistance of body.
Human skin has the characteristic of lipid membrane, is easy to absorb animal fat, vegetable fat, volatile liquid substance, also
There are the substances such as liposoluble vitamin.Prinsepia utilis royle oil contains these compositions, and winter trick is dry, cracks, gets chilblains, and uses prinsepia utilis royle oil
It is soft that smearing can make skin moisten, and can treat pernio, is good natural skin care cosmetic material, also has pole to hair maintenance
Good opsonic action.
Although prinsepia utilis royle oil being widely used in the industries such as food, health care, cosmetics, there is good heat-clearing solution
Poison, anti-inflammatory, promoting blood circulation, relieve pain, help digestion, stomach invigorating the effects of, but since prinsepia utilis royle oil is that a vegetable oil stability is poor, less
It is conveniently directly appended in aqua product, is extremely limited in cosmetics.Therefore, by lipid to prinsepia utilis royle oil
It is embedded, prepares prinsepia utilis royle oil nano liposomes, can be improved the stability of prinsepia utilis royle oil, slow release effect and conveniently in water
It is used in dispersion liquid.
Liposome technology is the nano-lipid carrier system for being applied to cosmetic field earliest.Since its special phosphatide is double
Molecular layer structure, can carry various hydrophilic, hydrophobic or amphiphilic substances, these substances are typically entrapped within liposome interior water
Phase, or insertion class lipid bilayer, or absorption are attached at the surface of liposome.The hydrophily vesica special construction of liposome, energy
It is enough to improve the stability for being wrapped by ingredient, the Transdermal absorption for promoting active constituent, extend action time, part is targeted and is made
With, reduce toxic side effect etc..
Patent CN200810168212.5 disclose it is a kind of using lipid complex as the paclitaxel submicron emulsion of intermediate vector, with
Paclitaxel lipid complexes are intermediate vector, and taxol is dissolved in oily phase, water phase, emulsifier, assistant for emulsifying agent is added.Emulsion droplet grain
Diameter is in 600nm or less；It is oily that mutually example is 5: 95 to 35: 65 compared with water；In terms of taxol, drugloading rate 0.25mg/ml-5mg/
ml.Manufactured Submicron Emulsion drugloading rate height, resistance to pressure sterilizing, stable quality after long time storage.It can be directly prepared into for intravenous drip
Infusion, can also freeze-dried technique be made and do cream, face the used time add physiological saline or grape glucose dilution after intravenous drip.
Patent CN200510029633.6 discloses a kind of solid taxol lipoid that can be used for injecting and taking orally
Nanoparticle and its solid pharmaceutical preparation.Its feature be with carrier material, surfactant package taxanes substance be made partial size it is small,
The nanometer solid taxol lipoid particle that encapsulation rate is high, stability is good, toxicity is low, is easily surface modified.The purple of preparation
China fir alcohols material solid lipid nano granule can enhance the targeting to cancer cell, improve taxanes substance to tumour cell
Inhibitory or killing effect improves curative effect, substantially reduces toxic side effect, reduces drug resistance.
Patent CN200610087479.2 discloses the solid lipid nano granule and its system of a kind of radix scutellariae active skull cap components
Agent preparation method.Radix scutellariae active skull cap components are meant extracts isolated radix scutellariae cellulose content 50% or more from baikal skullcap root
Effective portion 50% or more of effective component and wogonin content 90% or more of active component or radix scutellariae cellulose content
The effective component of position or wogonin content 90% or more.Radix scutellariae active skull cap components water solubility and fat-soluble poor, with
Solution ph increase solubility and increase, but chemical degradation easily occurs in alkaline condition.Radix scutellariae active skull cap components physicochemical property
Deficiency make it that injecting and administering preparations can not be made, take orally after bioavilability it is lower.
Patent CN201010235402.1 discloses Neo-garcinolic acid solid lipid nano granule and preparation method thereof, the new rattan
Yellow acid solid lipid nano granule, the Neo-garcinolic acid including therapeutically effective amount, pharmaceutical phosphatide, surfactant and lipid material
Material.Solid lipid nano granule is made in Neo-garcinolic acid by the present invention, is improved the solubility of Neo-garcinolic acid, is reduced irritation, mentions
High bioavilability extends drug action time in vivo and plays targeting in body localized clusters.
The common preparation method of liposome has alcohol injection, vortex dispersion method, ultrasonic dispersion, reverse phase evaporation, this
A little methods often refer to the addition and evaporation process of organic solvent, and the process is more complicated, obtained liposomal particle size point
Cloth is uneven, and stability is general, and the encapsulation rate of active matter is not high, there are problems that drug leakage.
Summary of the invention
The purpose of the invention is to overcome the deficiencies of the prior art and provide a kind of high stable prinsepia utilis royle oil liposome
Preparation process prepares prinsepia utilis royle oil liposome using high/low temperature alternation and high-pressure homogeneous combination process, can solve green bur in this way
Oil makes in cosmetics extensively in stability, encapsulation rate, slow release effect and the problem of be conveniently added to water medium aspect
With.
Another object of the present invention is to provide application of the above-mentioned high stable prinsepia utilis royle oil liposome in cosmetics.
Realizing a kind of technical solution of above-mentioned purpose is: a kind of preparation process of high stable prinsepia utilis royle oil liposome, including
Following steps:
S1 adds 2~20 parts of lecithin, 0-5 parts of nonionic surfactant and 20~65 parts of stabilizer respectively
Add in a reservoir, makes lecithin, non-ionic surface living under 60~70 DEG C of temperature condition and the stirring condition of 200rpm/min
Property agent and stabilizer mixed dissolution are uniform；
S2 adds 1~20 part of prinsepia utilis royle oil in the mixed solution that step S1 is obtained, in 60~70 DEG C of temperature strip
Oily phase is uniformly mixing to obtain under the stirring condition of part and 200rpm/min；
10~46 parts of deionized water is heated to 70 DEG C, obtains water phase by S3；
S4, by step S2 obtain oil be mutually injected into the water phase that step S3 is obtained, 70 DEG C of temperature conditions with
It is sheared under the stirring condition of 200rpm/min with the shear rate of 5000rpm/min, obtains semi-transparent sticky prinsepia utilis royle oil lipid
Body colostrum；
High/low temperature alternation step: the prinsepia utilis royle oil liposome colostrum that step S4 is obtained is cooled to -20 DEG C, keeps 24 by S5
Hour, 45~55 DEG C then are warming up to again, 2min is sheared with the shear rate of 5000rpm/min；
High-pressure homogenization step: S6 carries out homogeneous, homogenization pressure 1000 to the resulting system of step S5 using high-pressure homogeneous
~1500bar, one cycle, drop temperature control are 15~25 DEG C, obtain the prinsepia utilis royle oil liposome in pale yellow transparent.
The preparation process of above-mentioned high stable prinsepia utilis royle oil liposome, wherein the nonionic surfactant is selected from and spits
Temperature 80, span 80, polysorbate60, this disk 60, polyglycereol 10- myristinate, polyglycereol 6- myristinate, polyglycereol 10- are hard
It is one or several kinds of in resin acid ester, polyglycereol 6- stearate, polyglycereol 10- laurate and polyglycereol 6- laurate.
The preparation process of above-mentioned high stable prinsepia utilis royle oil liposome, wherein the stabilizer is selected from glycerine, sorb
One of alcohol, pentaerythrite, butanediol, propylene glycol, cholesterol and phytosphingosine are several.
The preparation process of above-mentioned high stable prinsepia utilis royle oil liposome, wherein the average grain of the prinsepia utilis royle oil liposome
Diameter is 100~500nm, encapsulation rate > 98%.
A kind of of the invention application for additionally providing high stable prinsepia utilis royle oil liposome in cosmetics, the prinsepia utilis royle oil
Liposome adds in the formula of cosmetics, and the additive amount of the prinsepia utilis royle oil liposome is the 0.1 of the cosmetics gross mass
~20%.
Using the technical solution of the preparation process of high stable prinsepia utilis royle oil liposome of the invention a kind of, high/low temperature is used
Alternation method and high pressure homogenization method complex method wrap up prinsepia utilis royle oil, and this method committed step is: 1. liposome colostrum
After the completion of high temperature preparation, it is cooled to -20 DEG C and saves for 24 hours, be then warming up to 45-55 DEG C again, cold stage during being somebody's turn to do, phosphatide
The activity concentration of surrounding is higher, more enough after the phase inversion that heated up greatly to be wrapped in liposome, improves encapsulation rate；②
Homogenization is carried out to aforesaid liquid using high-pressure homogeneous device, and controlling drop temperature is 15-25 DEG C, lower material temperature out
Degree, makes system partial size be maintained at a lower range, while can effectively prevent the leakage of active matter, made from this method
Liposomal particle size is uniform, and stability is good, is free of organic reagent, easy to operate, can industrial applications.Therefore, using the composite square
Method wraps up prinsepia utilis royle oil, can be good at solving the problems, such as prepared by liposome tradition, moreover it is possible to improve encapsulation rate and stabilization
Property, its bioavailability is improved, to promote absorption of human body.The present invention is suitable for safe, continuous production, can solve green thorn
Fruit oil can be widely applied for all kinds of in stability, encapsulation rate, slow release effect and the problem of be conveniently added to water medium aspect
The preparation of cosmetics.
A kind of preparation process of high stable prinsepia utilis royle oil liposome of the invention, effectively increases the encapsulating of prinsepia utilis royle oil
Rate, encapsulation rate > 98%.Active matter prinsepia utilis royle oil loss through effectively coating is small, and stability is good.Any toxic side effect is not used
Organic solvent and additive, can directly act on as green safe cosmetics.Prinsepia utilis royle oil liposome is nanoscale, improves life
Object availability can allow it to be widely used in the preparation of all kinds of cosmetics with water arbitrarily than dissolving each other.
Using the technical solution of the application of high stable prinsepia utilis royle oil liposome of the invention in cosmetics, skin can be made to moisten
Pool is soft, and can treat pernio, also has fabulous opsonic action to hair maintenance.
Detailed description of the invention
Fig. 1 is the flow chart of the preparation process of high stable prinsepia utilis royle oil liposome of the invention；
Fig. 2 is the grain size distribution of prinsepia utilis royle oil liposome；
Fig. 3 is average grain diameter and PDI variation diagram of the prinsepia utilis royle oil liposome under different extension rates；
Fig. 4 be prinsepia utilis royle oil liposome under different conditions of storage (25 DEG C, 40 DEG C and illumination) average grain diameter variation diagram.
Specific embodiment
The present inventor is in order to preferably understand technical solution of the present invention, below by specifically real
It applies example and will be described in detail with reference to the accompanying drawings:
Embodiment 1:
Please refer to Fig. 1 and Fig. 2, a kind of preparation process of high stable prinsepia utilis royle oil liposome, comprising the following steps:
S1 adds 14 parts of lecithin, 55 parts of glycerine (stabilizer) and 1 part of cholesterol (stabilizer) respectively
In container, make lecithin, glycerine and cholesterol mixing molten under 70 DEG C of temperature condition and the stirring condition of 200rpm/min
Solution is uniform；
S2 adds 9 parts of prinsepia utilis royle oil in the mixed solution that step S1 is obtained, in 60 DEG C of temperature conditions and
Oily phase is uniformly mixing to obtain under the stirring condition of 200rpm/min；
21 parts of deionized water is heated to 70 DEG C, obtains water phase by S3；
S4, by step S2 obtain oil be mutually injected into the water phase that step S3 is obtained, 70 DEG C of temperature conditions with
It is sheared under the stirring condition of 200rpm/min with the shear rate of 5000rpm/min, obtains semi-transparent sticky prinsepia utilis royle oil lipid
Body colostrum；
High/low temperature alternation step: the prinsepia utilis royle oil liposome colostrum that step S4 is obtained is cooled to -20 DEG C, keeps 24 by S5
Hour, 45 DEG C then are warming up to again, 2min is sheared with the shear rate of 5000rpm/min；
High-pressure homogenization step: S6 carries out homogeneous to the resulting system of step S5 using high-pressure homogeneous, homogenization pressure is
1000bar, one cycle, drop temperature control are 20 DEG C, obtain the prinsepia utilis royle oil liposome in pale yellow transparent.The blueness bur
It is about 125nm, PDI (Poly dispersity index, polydispersion that oil lipidosome, which measures its partial size through partial size potentiometric analyzer,
Coefficient) it is 0.256.
Embodiment 2:
Please refer to Fig. 1 and Fig. 2, a kind of preparation process of high stable prinsepia utilis royle oil liposome, comprising the following steps:
S1, by 12 parts of lecithin, 12 parts of sorbierite (stabilizer), 26 parts of glycerine (stabilizer) and 1 part it is poly-
Glycerol 10- stearate (nonionic surfactant) adds in a reservoir respectively, in 65 DEG C of temperature condition and 200rpm/
Make lecithin, sorbierite, glycerine and polyglycereol 10- stearate mixed dissolution uniform under the stirring condition of min；
S2 adds 15 parts of prinsepia utilis royle oil in the mixed solution that step S1 is obtained, in 70 DEG C of temperature conditions and
Oily phase is uniformly mixing to obtain under the stirring condition of 200rpm/min；
34 parts of deionized water is heated to 70 DEG C, obtains water phase by S3；
S4, by step S2 obtain oil be mutually injected into the water phase that step S3 is obtained, 70 DEG C of temperature conditions with
It is sheared under the stirring condition of 200rpm/min with the shear rate of 5000rpm/min, obtains semi-transparent sticky prinsepia utilis royle oil lipid
Body colostrum；
High/low temperature alternation step: the prinsepia utilis royle oil liposome colostrum that step S4 is obtained is cooled to -20 DEG C, keeps 24 by S5
Hour, 50 DEG C then are warming up to again, 2min is sheared with the shear rate of 5000rpm/min；
High-pressure homogenization step: S6 carries out homogeneous to the resulting system of step S5 using high-pressure homogeneous, homogenization pressure is
1100bar, one cycle, drop temperature control are 17 DEG C, obtain the prinsepia utilis royle oil liposome in pale yellow transparent.The blueness bur
It is about 112nm, PDI (Poly dispersity index, polydispersion that oil lipidosome, which measures its partial size through partial size potentiometric analyzer,
Coefficient) it is 0.089.
Embodiment 3:
Please refer to Fig. 1 and Fig. 2, a kind of preparation process of high stable prinsepia utilis royle oil liposome, comprising the following steps:
S1, by 6 parts of lecithin, 30.5 parts of pentaerythrite (stabilizer), 1 part of phytosphingosine (stabilizer), 20
The butanediol (stabilizer) and 0.5 part this disk 60 (nonionic surfactant) of part add in a reservoir respectively, at 65 DEG C
Make lecithin, pentaerythrite, phytosphingosine, butanediol and this disk 60 mixed under the stirring condition of temperature condition and 200rpm/min
Conjunction is uniformly dissolved；
S2 adds 5 parts of prinsepia utilis royle oil in the mixed solution that step S1 is obtained, in 70 DEG C of temperature conditions and
Oily phase is uniformly mixing to obtain under the stirring condition of 200rpm/min；
37 parts of deionized water is heated to 70 DEG C, obtains water phase by S3；
S4, by step S2 obtain oil be mutually injected into the water phase that step S3 is obtained, 70 DEG C of temperature conditions with
It is sheared under the stirring condition of 200rpm/min with the shear rate of 5000rpm/min, obtains semi-transparent sticky prinsepia utilis royle oil lipid
Body colostrum；
High/low temperature alternation step: the prinsepia utilis royle oil liposome colostrum that step S4 is obtained is cooled to -20 DEG C, keeps 24 by S5
Hour, 47 DEG C then are warming up to again, 2min is sheared with the shear rate of 5000rpm/min；
High-pressure homogenization step: S6 carries out homogeneous to the resulting system of step S5 using high-pressure homogeneous, homogenization pressure is
1500bar, one cycle, drop temperature control are 23 DEG C, obtain the prinsepia utilis royle oil liposome in pale yellow transparent.The blueness bur
It is about 135nm, PDI (Polydispersity Index, polydisperse system that oil lipidosome, which measures its partial size through partial size potentiometric analyzer,
Number) it is 0.296.
Embodiment 4:
Please refer to Fig. 1 and Fig. 2, a kind of preparation process of high stable prinsepia utilis royle oil liposome, comprising the following steps:
S1, by 16 parts of lecithin, 14 parts of glycerine (stabilizer), 25 parts of butanediol (stabilizer) and 1 part of gallbladder
Sterol (stabilizer) adds in a reservoir respectively, makes lecithin under 65 DEG C of temperature condition and the stirring condition of 200rpm/min
Rouge, glycerine, butanediol and cholesterol mixed dissolution are uniform；
S2 adds 17 parts of prinsepia utilis royle oil in the mixed solution that step S1 is obtained, in 70 DEG C of temperature conditions and
Oily phase is uniformly mixing to obtain under the stirring condition of 200rpm/min；
27 parts of deionized water is heated to 70 DEG C, obtains water phase by S3；
S4, by step S2 obtain oil be mutually injected into the water phase that step S3 is obtained, 70 DEG C of temperature conditions with
It is sheared under the stirring condition of 200rpm/min with the shear rate of 5000rpm/min, obtains semi-transparent sticky prinsepia utilis royle oil lipid
Body colostrum；
High/low temperature alternation step: the prinsepia utilis royle oil liposome colostrum that step S4 is obtained is cooled to -20 DEG C, keeps 24 by S5
Hour, 50 DEG C then are warming up to again, 2min is sheared with the shear rate of 5000rpm/min；
High-pressure homogenization step: S6 carries out homogeneous to the resulting system of step S5 using high-pressure homogeneous, homogenization pressure is
1300bar, one cycle, drop temperature control are 19 DEG C, obtain the prinsepia utilis royle oil liposome in pale yellow transparent.The blueness bur
It is about 126.6nm, PDI (Polydispersity Index, polydispersion that oil lipidosome, which measures its partial size through partial size potentiometric analyzer,
Coefficient) it is 0.115.
Embodiment 5:
Please refer to Fig. 1 and Fig. 2, a kind of preparation process of high stable prinsepia utilis royle oil liposome, comprising the following steps:
S1, by 8 parts of lecithin, 38.5 parts of propylene glycol (stabilizer) and 0.5 part of 6- myristinate (nonionic table
Face activating agent) it adds respectively in a reservoir, make lecithin, third under 65 DEG C of temperature condition and the stirring condition of 200rpm/min
Two pure and mild 6- myristinate mixed dissolutions are uniform；
S2 adds 11 parts of prinsepia utilis royle oil in the mixed solution that step S1 is obtained, in 70 DEG C of temperature conditions and
Oily phase is uniformly mixing to obtain under the stirring condition of 200rpm/min；
42 parts of deionized water is heated to 70 DEG C, obtains water phase by S3；
S4, by step S2 obtain oil be mutually injected into the water phase that step S3 is obtained, 70 DEG C of temperature conditions with
It is sheared under the stirring condition of 200rpm/min with the shear rate of 5000rpm/min, obtains semi-transparent sticky prinsepia utilis royle oil lipid
Body colostrum；
High/low temperature alternation step: the prinsepia utilis royle oil liposome colostrum that step S4 is obtained is cooled to -20 DEG C, keeps 24 by S5
Hour, 46 DEG C then are warming up to again, 2min is sheared with the shear rate of 5000rpm/min；
High-pressure homogenization step: S6 carries out homogeneous to the resulting system of step S5 using high-pressure homogeneous, homogenization pressure is
1080bar, one cycle, drop temperature control are 24 DEG C, obtain the prinsepia utilis royle oil liposome in pale yellow transparent.The blueness bur
It is about 138nm, PDI (Polydispersity Index, polydisperse system that oil lipidosome, which measures its partial size through partial size potentiometric analyzer,
Number) it is 0.127.
Embodiment 6:
Please refer to Fig. 1 and Fig. 2, a kind of preparation process of high stable prinsepia utilis royle oil liposome, comprising the following steps:
S1 spits 10 parts of lecithin, 20 parts of propylene glycol (stabilizer), 10 parts of butanediols (stabilizer) and 1.5 parts
Warm 60 (nonionic surfactants) add in a reservoir respectively, in 65 DEG C of temperature condition and the stirring condition of 200rpm/min
Under keep lecithin, propylene glycol, butanediol and polysorbate60 mixed dissolution uniform；
S2 adds 10 parts of prinsepia utilis royle oil in the mixed solution that step S1 is obtained, in 70 DEG C of temperature conditions and
Oily phase is uniformly mixing to obtain under the stirring condition of 200rpm/min；
48.5 parts of deionized water is heated to 70 DEG C, obtains water phase by S3；
S4, by step S2 obtain oil be mutually injected into the water phase that step S3 is obtained, 70 DEG C of temperature conditions with
It is sheared under the stirring condition of 200rpm/min with the shear rate of 5000rpm/min, obtains semi-transparent sticky prinsepia utilis royle oil lipid
Body colostrum；
High/low temperature alternation step: the prinsepia utilis royle oil liposome colostrum that step S4 is obtained is cooled to -20 DEG C, keeps 24 by S5
Hour, 50 DEG C then are warming up to again, 2min is sheared with the shear rate of 5000rpm/min；
High-pressure homogenization step: S6 carries out homogeneous to the resulting system of step S5 using high-pressure homogeneous, homogenization pressure is
1250bar, one cycle, drop temperature control are 15 DEG C, obtain the prinsepia utilis royle oil liposome in pale yellow transparent.The blueness bur
It is about 112nm, PDI (Polydispersity Index, polydisperse system that oil lipidosome, which measures its partial size through partial size potentiometric analyzer,
Number) it is 0.149.
Referring to Fig. 3, the prinsepia utilis royle oil liposome that embodiment 6 is obtained, is diluted with water 10,20,50,100 and 200 respectively
Times, the average grain diameter of prinsepia utilis royle oil liposome has no significant change, and PDI index is also without significant change, prinsepia utilis royle oil liposome grain
It diameter size and is distributed in highly stable in diluted aqueous solution, illustrates that prinsepia utilis royle oil liposome can be miscible with water arbitrary proportion.
Referring to Fig. 4, (prinsepia utilis royle oil liposome is initial average in Fig. 4 for the prinsepia utilis royle oil liposome that embodiment 2 is obtained
Partial size is about 112nm) it is individually placed to store 28 days under 25 DEG C, 40 DEG C and illumination condition, with the extension of time, prinsepia utilis royle oil rouge
The average grain diameter of plastid is slightly increased, and is changed within the scope of 112-118nm, as a result illustrates 40 DEG C of high temperature and illumination, to green bur
The average grain diameter of oil lipidosome has no significant effect.Therefore, prinsepia utilis royle oil liposome has good stability.
High stable prinsepia utilis royle oil liposome of the invention can be in the application in cosmetics, using preparation process of the invention
Obtained prinsepia utilis royle oil liposome adds in the formula of cosmetics, and the additive amount of prinsepia utilis royle oil liposome is that the cosmetics are total
The 0.1~20% of quality.Skin can be made to moisten soft, and pernio can be treated, also have fabulous opsonic action to hair maintenance.
In conclusion the preparation process of high stable prinsepia utilis royle oil liposome of the invention, uses high/low temperature alternation method and height
Pressure homogeneous method complex method wraps up prinsepia utilis royle oil, effectively increases the encapsulation rate of prinsepia utilis royle oil, encapsulation rate > 98%.Through
The active matter prinsepia utilis royle oil loss effectively coated is small, and stability is good.Organic solvent and the addition of any toxic side effect is not used
Agent can be acted on directly as green safe cosmetics.Prinsepia utilis royle oil liposome is nanoscale, improves bioavilability, can be with water
Any ratio dissolves each other, and allows it to be widely used in the preparation of all kinds of cosmetics.
Those of ordinary skill in the art it should be appreciated that more than embodiment be intended merely to illustrate the present invention,
And be not used as limitation of the invention, as long as the change in spirit of the invention, to embodiment described above
Change, modification will all be fallen within the scope of claims of the present invention.
Claims (5)
1. the preparation process of high stable prinsepia utilis royle oil liposome, which comprises the following steps:
S1 adds 2~20 parts of lecithin, 0-5 parts of nonionic surfactant and 20~65 parts of stabilizer respectively
In container, make lecithin, nonionic surfactant under 60~70 DEG C of temperature condition and the stirring condition of 200rpm/min
It is uniform with stabilizer mixed dissolution；
S2 adds 1~20 part of prinsepia utilis royle oil in the mixed solution that step S1 is obtained, in 60~70 DEG C of temperature conditions and
Oily phase is uniformly mixing to obtain under the stirring condition of 200rpm/min；
10~46 parts of deionized water is heated to 70 DEG C, obtains water phase by S3；
S4, the oil that step S2 is obtained mutually is injected into the water phase that step S3 is obtained, in 70 DEG C of temperature condition and 200rpm/
It is sheared under the stirring condition of min with the shear rate of 5000rpm/min, obtains semi-transparent sticky prinsepia utilis royle oil liposome colostrum；
S5, high/low temperature alternation step: being cooled to -20 DEG C for the prinsepia utilis royle oil liposome colostrum that step S4 is obtained, kept for 24 hours,
45~55 DEG C then are warming up to again, 2min is sheared with the shear rate of 5000rpm/min；
S6, high-pressure homogenization step: using it is high-pressure homogeneous to the resulting system of step S5 carry out homogeneous, homogenization pressure be 1000~
1500bar, one cycle, drop temperature control are 15~25 DEG C, obtain the prinsepia utilis royle oil liposome in pale yellow transparent.
2. the preparation process of high stable prinsepia utilis royle oil liposome as described in claim 1, which is characterized in that the nonionic table
Face activating agent is selected from Tween 80, span 80, polysorbate60, this disk 60, polyglycereol 10- myristinate, polyglycereol 6- myristic acid
One in ester, polyglycereol 10- stearate, polyglycereol 6- stearate, polyglycereol 10- laurate and polyglycereol 6- laurate
Kind is several.
3. the preparation process of high stable prinsepia utilis royle oil liposome as described in claim 1, which is characterized in that the stabilizer choosing
From one of glycerine, sorbierite, pentaerythrite, butanediol, propylene glycol, cholesterol and phytosphingosine or several.
4. the preparation process of high stable prinsepia utilis royle oil liposome as described in claim 1, which is characterized in that the prinsepia utilis royle oil
The average grain diameter of liposome is 100~500nm, encapsulation rate > 98%.
5. application of the high stable prinsepia utilis royle oil liposome as described in claim 1 in cosmetics, which is characterized in that the blueness
Bur oil lipidosome adds in the formula of cosmetics, and the additive amount of the prinsepia utilis royle oil liposome is the cosmetics gross mass
0.1~20%.
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Address after: 650106 No. 53, Ke Yan Road, hi tech Zone, Kunming, Yunnan. Applicant after: Yunnan Beitani Biotechnology Group Co.,Ltd. Applicant after: SHANGHAI BEITAINI BIOTECHNOLOGY Co.,Ltd. Address before: 650000 Kunming hi tech Zone, Yunnan Province, Department of medicine No. 53 Applicant before: KUNMING BEITAINI BIOTECHNOLOGY CO.,LTD. Applicant before: SHANGHAI BEITAINI BIOTECHNOLOGY Co.,Ltd. |
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Application publication date: 20190409 |