CN109568226A - A kind of aloe stem cell extract - Google Patents
A kind of aloe stem cell extract Download PDFInfo
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- CN109568226A CN109568226A CN201811426040.7A CN201811426040A CN109568226A CN 109568226 A CN109568226 A CN 109568226A CN 201811426040 A CN201811426040 A CN 201811426040A CN 109568226 A CN109568226 A CN 109568226A
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- Prior art keywords
- stem cell
- aloe
- cell extract
- aloe stem
- culture
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/20—Hypnotics; Sedatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Organic Chemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medical Informatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Anesthesiology (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides a kind of aloe stem cell extracts, and by the way of plant in vitro culture, the aloe stem cell extract quality being prepared into is stablized, and facilitate the deep processing of derived product.
Description
Technical field
The present invention relates to a kind of plant stem cell extracts, and in particular to aloe stem cell extract belongs to biotechnology
Field.
Background technique
Plant stem cell is also known as separate living tissue, is relative in plant for the mature tissue of differentiation and maturation
's.Plant meristem can be divided into apical meristem and lateral meristem two major classes according to its position.Wherein top point
Raw tissue includes shoot apical meristem (SAM) and Root apical meristem (RAM).Lateral meristem includes vascular cambium
(vascular cambium) and phellogen (cork cambium).
Early in 1918, U.S. Food and Drug Administration was it is confirmed that aloe is edible.Nowadays, aloe gel product has been
It is widely used in the production of the food such as beverage, jelly, Yoghourt, can.
The polysaccharide and multivitamin contained in aloe has good nutrition to human skin, moistens, whitening effect.Emerald green Ye Lu
Luxuriant growth is the Fresh leaf of aloe of the direct beauty of optimum, i.e. aloe barbadensis Miller, it have make skin contraction, softness, moisturizing, anti-inflammatory,
The performance of bleaching.There are also hardening, angling, the effect for improving scar is released, small wrinkle, eye pouch, cutis laxa can not only be prevented, also
It is able to maintain skin wet, delicate, at the same time it can also treat scytitis, to acne, freckle, acne and scald, knife wound, worm
Stinging etc. also has good curative effect.It is similarly effective to hair, hair holding can be made to moisten smooth, prevented hair loss.
Substance needed for aloe contains 75 kinds of elements, with human body cell almost coincide, and has apparent health value, quilt
People's honor is known as " magical plant ", " first-aid kit for domestic use ".But aloe is in the breeding process, relatively high to environmental requirement, and happiness temperature is cold,
It stops growing when temperature is reduced to 15 DEG C, starts death when being down to 0 DEG C or less, therefore northern area must greenhouse gardening or room
Interior potting.Aloe is the torrid zone, subtropical zone heliophile, and growth requires have sufficient sunlight, air.Meanwhile aloe is the torrid zone again
Desert succulent plant avoids ponding, humidity, stuffy ventilative.
In the breeding process due to aloe, slow growth, and it is higher to environmental requirement, it directly processes after picking using past
It is past to there are problems that wasting serious and unstable quality.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of aloe stem cell extracts.
Aloe stem cell extract provided by the invention, is prepared via a method which:
(1) routine in vitro culture plant cell and culture is collected；
(2) destruction step (1) obtains culture simultaneously collects supernatant；
(3) ingredient that supernatant middle-molecular-weihydroxyethyl is less than 3500Da (preferably smaller than 3000Da) is collected, and is optionally carried out cold
It is lyophilized dry.
Herein, " in vitro culture " of plant is using the liquid or solid culture medium manually prepared, to plant cell
Carry out the process of Multiplying culture.The culture medium of extracorporeal culturing plant is known to the technical staff of culture plant cell field, can
Be suitable for different culture mediums are selected with the type according to different plant cells.Because the present invention is needed using in growth
Cell, thus preferably herein, " in vitro culture " of plant do not include develop into the process of plant (plant regeneration), and
Only need the proliferation process of " in vitro culture " of conventional plant.Certainly, the normal growth stage of in vitro culture can be simulated
In plant cell can also be equal use.
Preferably, in step (1), cultivation temperature is 25~29 DEG C, the pH value 6.0~6.5 of culture, incubation time 10
~18 days.
Preferably, in step (2), broken is that freeze thawing is broken, and 2~5 freeze thawing are broken repeatedly.
Preferably, the collection in step (3) be membrane filtration collect, such as with molecular cut off be 3500Da (preferably
Filter membrane 3000Da) is collected.
The present invention can also be processed into suitable oral preparation, and acceptable auxiliary material is such as added, as filler, colorant,
Flavoring agent, excipient, essence etc..
Common acceptable supplementary product kind is various, and the type that can commercially obtain just has thousands of kinds.
The present invention can also be prepared into skin care item, and health-care effect is big mainly for adult, especially life stress, the salary rank stayed up late
Layer, especially women, acceptable auxiliary material are selected from one of vitamin E, yeast extract and water or a variety of, more preferably protect
Acceptable auxiliary material is vitamin E, yeast extract and water in skin product.
It is that quality of making the life better such as enhances energy that the present invention, which also has, improves sleep quality, reduces skin speckle and wrinkle
The effect of.
Product of the present invention quality is stablized, and effect is obvious, and preparative-scale is big, stable production process, simplicity.
Specific embodiment
The present invention will be described in detail by specific embodiment below.It is important to note that these are retouched
It states and is only exemplary description, and be not meant to limit the scope of the invention.
Embodiment 1
The preparation of aloe Stem Cell Activity ingredient
It takes aloe children's column to carry out callus tissue culture and squamous subculture, then embryo callus is taken to train in fluid nutrient medium
It supports, but without plant regeneration process, wherein the difference is that the process cultivated in liquid medium is, inoculum concentration 5g,
Cultivation temperature is 27 ± 0.5 DEG C, and pH value maintains 6.2~6.4, after thus cultivating 14 days, that is, it is thin that solid is collected by centrifugation
Born of the same parents' culture.After the culture brine of collection, freeze in -20 DEG C, is then melted in 20 DEG C, repeatedly
Supernatant is collected by centrifugation in freeze thawing tertiary crushing cell, crosses the membrane filtration that molecular cut off is 3500Da, collects filtered solution, cold
It is lyophilized dry, analysis of components shows wherein comprising a variety of amino acid, lignin and aloietic acid etc..
The health-care efficacy of active constituent in skin-protection product prepared by the present invention
In the case where informed consent, by 20 (female) ages 30-40 years old wage-earners volunteer (10 male, 10
Female), it is randomly divided into 3 groups:
1: 8 people of group (4 male, 4 female) once a day, is used continuously 20 days using the product of the embodiment of the present invention 1, and
Started daily to score to its health status at the 10th day, including routine work energy state (10 points), sleep quality (10
Point), whitening skin state (10 points) calculates 10 days average marks.
2: 6 people of group (3 male, 3 female), using the product of the embodiment of the present invention 1, twice daily, are carried out continuously 20
It, and started daily to score to its health status at the 10th day, including routine work energy state (10 points), matter of sleeping
It measures (10 points), whitening skin state (10 points), calculates 10 days average marks.
3: 6 people of group (3 male, 3 female), without using the product of the embodiment of the present invention 1, are carried out continuously 20 days, and
Started to score to it at the 10th day, standards of grading are the same as group 1.
During testing and carrying out, experimental result is as follows, organizes 1 and is equally divided into 16.5 points；Group 2 is equally divided into 25.8 points
；Group 3 is equally divided into 10.1 points.This shows that can make the life better quality once a day using product of the present invention, if can be daily
This product is used twice, and quality of life can be made obviously more preferable.
Wherein, the formula of skin care item is as follows:
1 health caring product prescription table of table
。
Claims (4)
1. a kind of aloe stem cell extract, which is characterized in that be prepared via a method which:
(1) routine in vitro culture plant cell and culture is collected；
(2) destruction step (1) obtains culture simultaneously collects supernatant；
(3) ingredient that supernatant middle-molecular-weihydroxyethyl is less than 3500Da is collected, and is optionally freeze-dried.
2. aloe stem cell extract according to claim 1, which is characterized in that in step (1), cultivation temperature 25
~29 DEG C, pH value 6.0~6.5 is cultivated, incubation time is 10~18 days.
3. aloe stem cell extract according to claim 1, which is characterized in that in step (2), broken is that freeze thawing is broken
It is broken, 2~5 times repeatedly.
4. aloe stem cell extract according to claim 1, which is characterized in that the mode of the collection in step (3)
It is that membrane filtration is collected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811426040.7A CN109568226A (en) | 2018-11-27 | 2018-11-27 | A kind of aloe stem cell extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811426040.7A CN109568226A (en) | 2018-11-27 | 2018-11-27 | A kind of aloe stem cell extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109568226A true CN109568226A (en) | 2019-04-05 |
Family
ID=65924456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811426040.7A Pending CN109568226A (en) | 2018-11-27 | 2018-11-27 | A kind of aloe stem cell extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109568226A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960718A (en) * | 2012-07-16 | 2013-03-13 | 赫康贸易(上海)有限公司 | Health products of plant stem cell |
| KR20150115703A (en) * | 2015-09-23 | 2015-10-14 | (주)아모레퍼시픽 | Composition for promoting the volume of skin |
| CN105852125A (en) * | 2016-04-27 | 2016-08-17 | 深圳爱生再生医学科技有限公司 | Aloe stem cell lyophilized powder as well as preparation method and application thereof |
| CN105962367A (en) * | 2016-04-27 | 2016-09-28 | 深圳爱生再生医学科技有限公司 | Aloe stem cell extract as well as preparation method and application thereof |
-
2018
- 2018-11-27 CN CN201811426040.7A patent/CN109568226A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960718A (en) * | 2012-07-16 | 2013-03-13 | 赫康贸易(上海)有限公司 | Health products of plant stem cell |
| KR20150115703A (en) * | 2015-09-23 | 2015-10-14 | (주)아모레퍼시픽 | Composition for promoting the volume of skin |
| CN105852125A (en) * | 2016-04-27 | 2016-08-17 | 深圳爱生再生医学科技有限公司 | Aloe stem cell lyophilized powder as well as preparation method and application thereof |
| CN105962367A (en) * | 2016-04-27 | 2016-09-28 | 深圳爱生再生医学科技有限公司 | Aloe stem cell extract as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 国家药典委员会: "《中华人民共和国药典临床用药须知：2015年版.中药饮片卷》", 30 September 2017, 中国医药科技出版社 * |
| 张清: "《高血脂饮食+运动+中医调养全书》", 30 April 2016, 天津科学技术出版社 * |
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| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190405 |
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