CN105962367A - Aloe stem cell extract as well as preparation method and application thereof - Google Patents
Aloe stem cell extract as well as preparation method and application thereof Download PDFInfo
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- CN105962367A CN105962367A CN201610268507.4A CN201610268507A CN105962367A CN 105962367 A CN105962367 A CN 105962367A CN 201610268507 A CN201610268507 A CN 201610268507A CN 105962367 A CN105962367 A CN 105962367A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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Abstract
The invention relates to a preparation method of an aloe stem cell extract. The preparation method comprises the following steps of obtaining tissue containing formation layers from aloe; placing the tissue containing formation layers in solid mediums for culturing, and separating aloe stem cells; then placing the aloe stem cells in plant stem cell culture mediums for culture in vitro so as to obtain amplified aloe stem cells; and freezing the amplified aloe stem cells, crushing the frozen amplified aloe stem cells, and performing concentration so as to obtain the aloe stem cell extract. The aloe stem cell extract obtained by the method contains high aloe active components particularly barbaloin, and has a high medical value.
Description
Technical field
The present invention relates to biological technical field, particularly to a kind of Aloe stem cell extract and preparation side thereof
Method and application.
Background technology
Plant stem cell (plant stem cell) is undifferentiated cell, has the strongest self renewal and holds
Continuous splitting ability.Plant stem cell culture technique, is on the basis of tradition tissue culture technology, does with plant
Cell is target, induces, separates and cultivate outer implant, sets up corresponding stem cell cultivating system plant and does
Cell culture technology, not only enriches vegetable culture technique, and is commercially producing of natural product,
Or even the development of Plant Biotechnology, it is provided that new research direction and opportunity.
Aloe is a kind of perennial meat herbaceous plant, belongs to Liliaceae Aloe, traditionally as cathartic
Use, in recent years, be also widely used in cosmetics and food service industry.Deep along with study
Enter, the oxidation resistance of Aloe, antibacterial, antiinflammatory, antitumor, immunomodulating and auxiliary treatment diabetes
Effect the most in succession be in the news out.Aloin A, wound hormone and polysaccharide peptide manna in Aloe etc. have
Have viral infection resisting, promote wound healing restore effect, again can with anti-inflammation and sterilization, heat absorption detumescence, soft
Changing skin, keep cell viability, curdlan combines the effect also with healing wounds activity with 1-decene-1.
Therefore, how to prepare Aloe stem cell extract to have great importance.
Summary of the invention
In consideration of it, the present invention provides a kind of Aloe stem cell extract and preparation method thereof.
The technical solution adopted for the present invention to solve the technical problems is: a kind of Aloe stem cell extract
Preparation method, comprises the following steps:
Obtain containing cambial tissue from Aloe；
By described containing cambial tissue be placed in solid medium cultivation after, isolate Aloe stem cell；
Again described Aloe stem cell is placed in plant stem cell culture medium and carries out In vitro culture, it is thus achieved that amplification
After Aloe stem cell；
Aloe stem cell after described amplification chilled broken after concentrate, i.e. results Aloe stem cell extract.
In the preparation method of the Aloe stem cell extract of present invention offer, described freezing crushing process is:
Aloe stem cell after described amplification is put into freezing 2～3 hours under-50～-100 DEG C of low temperature environments, then takes
Go out to thaw, repeat 3～5 times.
In the preparation method of the Aloe stem cell extract of present invention offer, described plant stem cell is cultivated
Base includes inorganic salt, vitamin, aminoacid, auxin, saccharide and agar.
In the preparation method of the Aloe stem cell extract of present invention offer, described solid medium includes
Inorganic salt, vitamin, aminoacid, auxin, saccharide and activated carbon.
In the preparation method of the Aloe stem cell extract of present invention offer, in described solid medium also
Including naphthalene acetic acid and benzyl aminoadenine.
In the preparation method of the Aloe stem cell extract of present invention offer, described saccharide is sucrose or breast
Sugar.
In the preparation method of the Aloe stem cell extract of present invention offer, described Process of in vitro is
After cultivating 7～14 days in constant-temperature shaking incubator, transfer to bioreactor is carried out large-scale culture.
In the preparation method of the Aloe stem cell extract of present invention offer, the condition of described In vitro culture
It is 30 DEG C～37 DEG C for temperature, illumination per hour 5～10min.
The Aloe that the present invention protects the preparation method of above-mentioned Aloe stem cell extract to be obtained further is dry thin
Born of the same parents' extract.
The present invention protect further above-mentioned Aloe stem cell extract prepare food, medicine, health product or
Application in cosmetics.
Implement the present invention provide Aloe stem cell extract and preparation method, can reach following has
Benefit effect: by the active aloe composition contained by the Aloe stem cell extract that the present invention is obtained relatively
Height, particularly barbaloin, have higher use value.
Detailed description of the invention
The preparation method of the Aloe stem cell extract that the present invention provides, comprises the following steps:
S1, obtain containing cambial tissue from Aloe；
S2, by step S1 obtains be placed in containing cambial tissue solid medium cultivated after,
Isolate Aloe stem cell；
S3, the Aloe stem cell obtained in step S2 is placed in plant stem cell culture medium and carries out external training
Support, it is thus achieved that the Aloe stem cell after amplification；
Wherein, described plant stem cell culture medium include inorganic salt, vitamin, aminoacid, auxin,
Saccharide and agar.Wherein, inorganic salt includes: CaCl2、KH2PO4、KNO3、MgSO4、NH4NO3、
CuSO4·5H2O etc.；Saccharide is sucrose or lactose etc.；Preferably, in plant stem cell culture medium, raw
The mass fraction that concentration is 0.5～1.5mg/L, sucrose or lactose of long element is 2～5%, and the concentration of agar is
5.5～7.5g/L；Plant stem cell culture medium is MS culture medium.
After the amplification that S4, step S3 obtain Aloe stem cell chilled broken after concentrate, i.e. results Aloe does
Cell extract.
In step S1, owing to Aloe stem apex having self-renewal capacity and can produce containing substantial amounts of again and have
Continue the stem cell of splitting ability, therefore, the present invention preferentially uses acquisition Aloe from Aloe stem apex do
Cell.Containing the acquisition process of cambial tissue, comprise the following steps:
Take Aloe stem apex, rinse 15～20 minutes, alcohol disinfecting, then with mercuric chloride liquid disinfectant 10～20 minutes
After, aseptic water washing for several times, is peeled off and is obtained cambial tissue.
In step S2, solid medium include inorganic salt, vitamin, aminoacid, auxin, saccharide and
Activated carbon；Wherein, inorganic salt includes: CaCl2、KH2PO4、KNO3、MgSO4、NH4NO3、
CuSO4·5H2O etc.；Saccharide is sucrose or lactose etc..Preferably, solid medium is MS culture medium；
And the mass fraction that the concentration of auxin is 0.5～1.5mg/L, sucrose or lactose is 2～5%；It is more highly preferred to
Ground, also includes naphthalene acetic acid and benzyl aminoadenine in solid medium, the concentration of naphthalene acetic acid is
0.5～1.5mg/L, the concentration of benzyl aminoadenine is 0.5～1.5mg/L.
Further, in this step, carry out cultivating by being placed in solid medium containing cambial tissue
Condition is: cultivation temperature is 23～29 DEG C, illumination 10～12 hours, intensity of illumination 1500～2000lx, PH
Value is 5.6～5.9, cultivates 7～14 days.
Further, after solid medium is cultivated, well-grown shape is obtained containing cambial tissue
Stratification stem cell group, in addition it is also necessary to use enzymatic isolation method to isolate Aloe stem cell.The present invention preferentially uses quality
Mark is 0.2-0.3% cellulase solution and mass fraction is that cambium layer is done by 0.2-0.3% pectinase solution
Cell mass carries out enzymolysis 8～12 hours.
In step S3, the process of In vitro culture Aloe stem cell is carried out in constant-temperature shaking incubator, shakes
Bed rotating speed is 100 revs/min～120 revs/min, and temperature is 30 DEG C～37 DEG C, illumination per hour
5～10min, after incubation time is 7 days～14 days, obtain single cell suspension.Then by single cell suspension
It is transferred to bioreactor and carries out large-scale culture, when cultivating the 4th～7 day in bioreactor, add
Fresh plant stem cell culture medium, the Aloe stem cell after results expand after continuing to cultivate.
In step S4, the Aloe stem cell after the amplification that will obtain in step S3 puts into-50～-100 DEG C of low temperature
Under environment, freezing 2～3 hours, then take out defrosting, repeat 3～5 times, with ultrasonic after last defrosting
Ripple crushes instrument smudge cells, collects filtrate, is evaporated in vacuo concentration, i.e. obtains Aloe stem cell extract.
The Aloe stem cell extract that the present invention protects said method to be obtained further is preparing food, medicine
Application in product, health product or cosmetics.
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment,
The present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to
Explain the present invention, be not intended to limit the present invention.
Embodiment 1
In the present embodiment, the preparation method of Aloe stem cell extract, comprise the following steps:
S1, obtain containing cambial tissue from Aloe；
Select without fertilizer and pesticide pollution, the Aloe of green organic plantation, and choose upgrowth situation preferably reed
Luxuriant growth stem apex, with aseptic water washing Aloe stem apex surface attachments 15～20 minutes, then enters with the ethanol of 75%
Row surface sterilization 30 seconds, then, the mercuric chloride liquid disinfectant 10 with 0.1%～20 minutes, finally anti-with sterilized water
Rinse 5 times again, wash away the mercuric chloride residual liquid of plant surface；Aloe stem apex after sterilizing is cut into 5mm × 5mm
Size, separates containing cambial tissue with vaccinating lancet.
S2, will be placed in solid medium containing cambial tissue and cultivate, isolate Aloe dry thin
Born of the same parents；
It is seeded to what step S1 separated in solid medium containing cambial tissue, with every 1g
Tissue inoculation 5cm2The density of solid medium is inoculated, and after cultivation, Cambium tissue is homogeneous growth
Tabular tissue, its hetero-organization is then irregular gathering growth-gen；Cambium tissue by regeneration
Open with other separate tissue, with every 1g tissue inoculation 5cm2The density of solid medium, by the shape of regeneration
Layer tissue is become to be seeded in fresh solid medium cultivate.Wherein, in solid medium possibly together with
Concentration is the naphthalene acetic acid of 1.0mg/L and benzyl aminoadenine that concentration is 1.0mg/L, and solid medium is
MS culture medium, is the CaCl of 232mg/L containing concentration2, the KH of 140mg/L2PO4、1642mg/L
KNO3, the MgSO of 130.54mg/L4, the NH of 1560mg/L4NO3, 0.021mg/L
CuSO4·5H2The vitamin C of O, 60mg/L, the auxin of the glycine of 2mg/L, 1.0mg/L,
Mass fraction is sucrose and the activated carbon of 3%；Condition of culture is: temperature 26 DEG C, illumination 11 hours, light
Being 1800lx according to intensity, pH value is 5.6～5.9, cultivates 14 days；Cambium tissue source can be gathered in the crops
Aloe stem cell group；It is the fiber of 0.25% with the pectinase solution that mass fraction is 0.25% and mass fraction
Element enzyme carries out enzymolysis 12 hours to Aloe stem cell group, isolates Aloe stem cell.
S3, Aloe stem cell is placed in plant stem cell culture medium carries out In vitro culture；
Aloe stem cell after enzymolysis in step S2 is transferred in plant stem cell culture fluid, is placed in perseverance
Temperature is cultivated and is cultivated on shaking table.
Wherein, plant stem cell culture fluid is MS culture medium, and wherein containing concentration is 372mg/L's
CaCl2, the KH of 181mg/L2PO4, the KNO of 1790mg/L3, the MgSO of 173.54mg/L4、
The NH of 1840mg/L4NO3, the CuSO of 0.022mg/L4·5H2The vitamin C of O, 60mg/L,
The glycine of 1.58mg/L, the auxin of 1.5mg/L, mass fraction be 3% sucrose and concentration be 6.0g/L
The additive such as agar；The rotating speed of shaking table is 110 revs/min, and cultivation temperature is 34 DEG C, illumination per hour
8 minutes, cultivating the 7th day, adding fresh plant stem cell culture fluid to cell density is 1 × 107Individual/ml,
Then proceed to cultivate, with the stainless steel sift net filtration of 100 mesh after cultivation, remove cell mass, residue etc.,
Obtain Aloe stem cell suspension；
Then, Aloe stem cell suspension is transferred in 20L bioreactor carry out large-scale culture, raw
Thing reactor is added with the plant stem cell culture medium of 5L, when cultivating the 7th day, adds the fresh of 10L
Culture medium, harvesting after continuing to cultivate.Matched group is cultivated and is persistently cultivated in bioreactor 20 days,
Middle without culture medium.Harvesting after cultivation, weighs.
| Detection object | Cell weight (unit: gram) |
| Embodiment 1 | 1.572 |
| Matched group | 0.539 |
Step S4, the preparation of Aloe stem cell extract；
Collect and step S3 obtain Aloe stem cell suspension, filter with 1um filter, remove culture medium,
The cell that results retain；And cell is put into the cryotherapy 2 hours of-80 DEG C, then take out defrosting 30
Minute, repeat aforesaid operations 5 times, after last thaw at RT, use sonicator smudge cells,
After filter filters, collect filtrate, concentrate in rotating vacuum evaporator, Aloe can be obtained dry thin
Born of the same parents' extract, employing HPLC method measures the content of barbaloin in Aloe stem cell extract and is
150.80mg/g；And directly from aloe extraction liquid, the content of barbaloin is only 32.35mg/g.
Above embodiments of the invention are described, but the invention is not limited in above-mentioned concrete
Embodiment, above-mentioned detailed description of the invention is only schematic rather than restrictive, this area
Those of ordinary skill under the enlightenment of the present invention, without departing from present inventive concept and claimed
Ambit under, it may also be made that a lot of form, within these belong to the protection of the present invention.
Claims (10)
1. the preparation method of an Aloe stem cell extract, it is characterised in that comprise the following steps:
Obtain containing cambial tissue from Aloe；
By described containing cambial tissue be placed in solid medium cultivation after, isolate Aloe stem cell；
Again described Aloe stem cell is placed in plant stem cell culture medium and carries out In vitro culture, it is thus achieved that amplification
After Aloe stem cell；
Aloe stem cell after described amplification chilled broken after concentrate, i.e. results Aloe stem cell extract.
The preparation method of Aloe stem cell extract the most according to claim 1, it is characterised in that
Described freezing crushing process is: put under-50～-100 DEG C of low temperature environments by the Aloe stem cell after described amplification
Freezing 2～3 hours, then take out defrosting, repeat 3～5 times.
The preparation method of Aloe stem cell extract the most according to claim 1, it is characterised in that
Described plant stem cell culture medium includes inorganic salt, vitamin, aminoacid, auxin, saccharide and agar.
The preparation method of Aloe stem cell extract the most according to claim 1, it is characterised in that
Described solid medium includes inorganic salt, vitamin, aminoacid, auxin, saccharide and activated carbon.
The preparation method of Aloe stem cell extract the most according to claim 4, it is characterised in that
Described solid medium also includes naphthalene acetic acid and benzyl aminoadenine.
6., according to the preparation method of the Aloe stem cell extract described in claim 3 or 4, its feature exists
In, described saccharide is sucrose or lactose.
The preparation method of Aloe stem cell extract the most according to claim 1, it is characterised in that
Described Process of in vitro is to cultivate after 7～14 days in constant-temperature shaking incubator, transfers to bioreactor
In carry out large-scale culture.
The preparation method of Aloe stem cell extract the most according to claim 7, it is characterised in that
The condition of described In vitro culture be temperature be 30 DEG C～37 DEG C, illumination per hour 5～10min.
9. the preparation method institute according to the Aloe stem cell extract described in any one of claim 1～8
The Aloe stem cell extract obtained.
Aloe stem cell extract the most according to claim 9 is preparing food, medicine, health care
Application in product or cosmetics.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107259325A (en) * | 2017-07-27 | 2017-10-20 | 灵山县龙璇蛇业有限公司 | A kind of snake blood rice and preparation method thereof |
| CN109568226A (en) * | 2018-11-27 | 2019-04-05 | 山东奥博森生物药业股份有限公司 | A kind of aloe stem cell extract |
| CN110294863A (en) * | 2019-06-12 | 2019-10-01 | 深圳爱生再生医学科技有限公司 | A kind of polypeptide and its derivative, application, nucleotide sequence, recombinant expression carrier, composition |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102459572A (en) * | 2009-05-26 | 2012-05-16 | 云火公司 | Plant stem cell derived from cambium of family gingkoaceae and method for isolation thereof |
| CN103509749A (en) * | 2012-06-29 | 2014-01-15 | 鹭港生物药业有限公司 | Separation and culture method using ginseng cambium stem cells |
| CN104498425A (en) * | 2014-11-28 | 2015-04-08 | 广州赛莱拉干细胞科技股份有限公司 | Isolated culture method of saussurea involucrata stem cells and application of saussurea involucrata stem cells in preparation of anti-senile product |
| CN104711215A (en) * | 2015-04-09 | 2015-06-17 | 广州赛莱拉干细胞科技股份有限公司 | Apple stem cell culture method and apple stem cells cultured by method |
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
-
2016
- 2016-04-27 CN CN201610268507.4A patent/CN105962367A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102459572A (en) * | 2009-05-26 | 2012-05-16 | 云火公司 | Plant stem cell derived from cambium of family gingkoaceae and method for isolation thereof |
| CN102459572B (en) * | 2009-05-26 | 2014-11-19 | 云火公司 | Plant stem cell derived from cambium of family gingkoaceae and method for isolation thereof |
| CN103509749A (en) * | 2012-06-29 | 2014-01-15 | 鹭港生物药业有限公司 | Separation and culture method using ginseng cambium stem cells |
| CN104498425A (en) * | 2014-11-28 | 2015-04-08 | 广州赛莱拉干细胞科技股份有限公司 | Isolated culture method of saussurea involucrata stem cells and application of saussurea involucrata stem cells in preparation of anti-senile product |
| CN104711215A (en) * | 2015-04-09 | 2015-06-17 | 广州赛莱拉干细胞科技股份有限公司 | Apple stem cell culture method and apple stem cells cultured by method |
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
Non-Patent Citations (2)
| Title |
|---|
| 孟广云等: "《野生蔬菜保鲜与加工技术》", 31 January 2007, 中国农业出版社 * |
| 杨平华: "《农田植物生长调节剂使用技术》", 31 January 2009, 四川科学技术出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107259325A (en) * | 2017-07-27 | 2017-10-20 | 灵山县龙璇蛇业有限公司 | A kind of snake blood rice and preparation method thereof |
| CN109568226A (en) * | 2018-11-27 | 2019-04-05 | 山东奥博森生物药业股份有限公司 | A kind of aloe stem cell extract |
| CN110294863A (en) * | 2019-06-12 | 2019-10-01 | 深圳爱生再生医学科技有限公司 | A kind of polypeptide and its derivative, application, nucleotide sequence, recombinant expression carrier, composition |
| CN110294863B (en) * | 2019-06-12 | 2021-06-11 | 深圳爱生再生医学科技有限公司 | Polypeptide and derivative, application, nucleotide sequence, recombinant expression vector and composition thereof |
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Application publication date: 20160928 |