CN109557029A - It is a kind of with temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device - Google Patents
It is a kind of with temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device Download PDFInfo
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- CN109557029A CN109557029A CN201811534018.4A CN201811534018A CN109557029A CN 109557029 A CN109557029 A CN 109557029A CN 201811534018 A CN201811534018 A CN 201811534018A CN 109557029 A CN109557029 A CN 109557029A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/171—Systems in which incident light is modified in accordance with the properties of the material investigated with calorimetric detection, e.g. with thermal lens detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/171—Systems in which incident light is modified in accordance with the properties of the material investigated with calorimetric detection, e.g. with thermal lens detection
- G01N2021/1714—Photothermal radiometry with measurement of emission
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Abstract
The invention belongs to technical field of analysis and detection more particularly to a kind of with temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device, and the detection device includes shell, controller, Dual channel detection module and temperature automatically controlled module.Dual channel detection module is made of two groups of symmetrical Opto-thertnal detection components, every group of Opto-thertnal detection component all includes colloidal gold strip, laser generator, infrared radiation temperature sensor, temperature automatically controlled module includes temperature sensor, heater, semiconductor cooling component, and controller controls Dual channel detection and temperature control modules.The arrangement achieves the thermostatic controls that the binary channels of detection sample colloidal gold strip and blank control sample colloidal gold strip detected and detected simultaneously environment temperature, compared with conventional single-channel temperature sensor Opto-thertnal detection, testing result is more sensitive and reliable, and operating process and data processing are simpler.
Description
Technical field
The invention belongs to technical field of analysis and detection more particularly to a kind of having for colloidal gold strip quantitative analysis
Temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device.
Background technique
Colloidal gold strip detection method is a kind of easily and efficiently detection method, operation letter wide with detection range
The advantages that single, at low cost, easy to carry, can be not only suitable for base's use, can also be answered extensively with long-term preservation under proper condition
For fields such as Food Inspection, hospital clinical and animal diseases diagnosis.
Colloidal gold strip generally passes through the colloidal gold color change visual display detecting result of p-wire in test strips, mesh
Measure qualitative detection as a result, however due to cannot achieve accurate quantification detection, limit colloid gold immune test paper item detection skill
The extensive use of art.In order to realize quantitative detection, colloid gold immune test paper quantitative measurement technology and device are carried out both at home and abroad
Research and development.Currently, mainly using photo resistance measurement scheme, mirror based fiber optica sensor measurement reflective light intensity scheme and image
Sensor acquires graphics solution, quantitative determines to colloidal gold color change.First two scheme sensitivity is limited, by environment shadow
It rings obviously, and imaging sensor acquisition graphics solution needs powerful data-handling capacity, need to configure more expensive specialized equipment, examines
Survey process complexity is cumbersome, still needs to develop new quantitative measurement technology.
Colloid gold nanoparticle has unique Photothermal characterisation, i.e., under the light irradiation of certain wavelength, colloidal gold nanoparticle
Surface plasma body resonant vibration occurs for son, and internal crystal framework is caused to resonate and generate a large amount of thermal energy immediately.Using in colloidal gold strip
The photo-thermal effect of colloid gold nanoparticle can realize quantifying for test strips by measuring the thermal exposure of colloidal gold color development area
Detection.And Opto-thertnal detection mainly has infrared imaging method and infrared temperature sensor method at present.Infrared imaging method is to colloidal gold part
Heat radiation in region measures, then the temperature profile data averaged in localized region, counter to push away colloidal gold
Content.But infrared imaging method has certain defect, first is that using infrared imaging method directly in colloidal gold regional area
Temperature is acquired, and carries out handling averagely, is easy to the interference by ambient temperature, and can not be embodied high after colloidal gold is stimulated
The advantage of infrared emanation;Second is that infrared imaging method data processing complex, device is larger and higher cost, does not meet quick detection
Requirement.Patent document 201710625736.1 disclose " it is a kind of based on photo-thermal effect detection colloidal gold strip quantitatively examine
Survey device and method ", a kind of method for directlying adopt infrared temperature sensor thermometric is proposed, the advantages of this method is temperature
The thermal exposure of colloidal gold is directly converted to single point temperature value by sensor, and avoiding infrared photography method need to be to entire colloidal gold spoke
Area's temperature is penetrated to be handled, thus have detect it is sensitive it is reliable, data processing is simple, compact structure is portable, it is cheap, be easy to
The features such as operation.
However, there are still some defects and deficiencies for the above-mentioned Opto-thertnal detection device based on infrared temperature sensor detection.One
The core detection physical quantity for being Opto-thertnal detection is temperature value, is highly prone to the interference of environment temperature, changeable environment temperature shadow
Its detection accuracy is rung.Second is that there is also ambient temperature, this background temperature under laser irradiation for the blank control of colloidal gold strip
Degree needs to be deducted, and current method detects sample and blank control using two-step method detection, on the one hand the method will lead to its inspection
Complex steps are surveyed, data processing complex is unfavorable for the exploitation of its equipment automatization;On the other hand in two step detection process due to
Environment temperature and the thermally equilibrated difference of detection device are detected, the measurement value drift for detecting sample and blank control is big, detection accuracy
It is greatly restricted with sensitivity.
Summary of the invention
It is a kind of with temperature automatically controlled binary channels the present invention intends to provide for above-mentioned problems of the prior art
Portable colloidal gold test paper strip Opto-thertnal detection device.Detection device of the invention is integrated with heater and peltier cooler, real
Show the temperature automatically controlled of detection device, solves the problems, such as the influence of environment temperature.Meanwhile detection device of the invention uses bilateral
Road detection system, detection while realizing detection sample and blank control sample, not only simplifies detecting step and data processing, disappears
The big problem of temperature drift is measured when except two-step method detection, and detection accuracy and sensitivity are greatly improved.
An object of the present invention is to provide a kind of with temperature automatically controlled binary channels portable colloidal gold test paper strip photo-thermal
Detection device.
It is above-mentioned with temperature automatically controlled binary channels portable colloidal gold test paper strip photo-thermal the second object of the present invention is to provide
The application of detection device skill.
For achieving the above object, the invention discloses following technical proposals:
Firstly, the invention discloses a kind of with temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection dress
It sets, including shell, controller, Dual channel detection module and temperature automatically controlled module.The Dual channel detection module is located in shell
Portion, the controller and temperature automatically controlled module are mounted on shell;The Dual channel detection module and temperature automatically controlled module with
Controller connection, realizes binary channels and detects automatically and temperature automatic control.
The Dual channel detection module includes: the detection group Opto-thertnal detection component being disposed adjacent and control group Opto-thertnal detection group
Part.
The detection group Opto-thertnal detection component includes: detection sample colloidal gold strip, the first infrared temperature sensor, first
Laser generator, first infrared temperature sensor and first laser generator are arranged on detection sample colloidal gold strip
Side, and the launch window of the induction window of the first infrared temperature sensor and first laser generator is towards detection sample colloidal gold
Test strips, first infrared temperature sensor and first laser generator are connected to controller.
The control group Opto-thertnal detection component include: blank control sample colloidal gold strip, the second infrared temperature sensor,
Second laser generator, second infrared temperature sensor and second laser generator are arranged at blank control sample colloidal gold
Above test strips, and the induction window of the second infrared temperature sensor and the launch window of second laser generator are towards blank
Control sample colloidal gold strip, second infrared temperature sensor and second laser generator are connected to controller.
Controller starts first laser generator and second laser generator simultaneously, issues identical specific wavelength laser and shines
The colloidal gold detection zone being mapped on detection sample colloidal gold strip and blank control sample colloidal gold strip, colloidal gold therein are inhaled
It receives luminous energy and induces infra-red radiation, the first infrared temperature sensor and the second infrared temperature sensor the amount of infrared radiation of detection zone
Temperature value is converted to, and sends controller to.Controller calculates the two difference, and according to the mark between temperature difference and sample concentration
Directrix curve determines the sample concentration of detection sample.
The temperature automatically controlled module includes temperature sensor, heater and chiller assembly, the temperature sensor setting
Inside housings, on an interior surface of the housing, the heater and temperature sensor connect with controller for the heater installation
It is logical.
The chiller assembly includes semiconductor cooling fin, heat-conducting plate, radiator fan;The heat-conducting plate is installed tightly to shell
In the window opened up on body, the semiconductor cooling fin is connect with heat-conducting plate, and is located in shell, and the radiator fan is located at shell
It outside body, and is connect with heat-conducting plate, heat is discharged outside shell;The semiconductor cooling fin and radiator fan and controller connect
It connects.
The temperature range of temperature and setting that controller is detected according to temperature sensor compares, when detection temperature is low
When set temperature lower limit, controller controls heater and is powered, temperature sensor real-time monitoring enclosure interior temperature in heating process
Degree reaches controller control heater power-off after set temperature range;When detecting temperature higher than the set temperature upper limit, controller
It controls semiconductor cooling fin and radiator fan is powered, temperature sensor real-time monitoring enclosure interior temperature in cooling procedure reaches
Controller control semiconductor cooling fin and radiator fan power-off after set temperature range.
Further, the controller is single-chip microcontroller or PLC or PC, and the temperature sensor is thermocouple.
Further, the wavelength of the first laser generator and second laser generator is green wavelength, and wavelength is optimal
It is selected as 532nm.
Further, the first laser generator, second laser generator are respectively relative to detection sample colloid gold test paper
Item, blank control sample colloidal gold strip are obliquely installed, and are more advantageous to colloidal gold strip in this way and are absorbed light, induce infrared spoke
It penetrates.
Further, the heater is heating sheet or heating film.
Further, the temperature sensor is thermocouple.
Secondly, the invention discloses described with temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection dress
Set the application being in the fields such as Food Inspection, hospital clinical and animal diseases diagnosis.
Compared with prior art, the present invention achieve it is following the utility model has the advantages that
(1) due to colloidal gold strip quantitative detection result accurately whether core detection physical quantity be temperature value, and ring
The little change and detection device thermal balance difference of border temperature can all significantly affect final measured value.The present invention is integrated with heating
Device and peltier cooler realize the Constant Temperature Detection of detection device, eliminate the variation of environment temperature to measurement temperature value
Negative influence.
(2) present invention using double-channel detector simultaneously to detection sample and blank control sample detect, directly output and
The temperature gap of the proportional relationship of colloidal gold amount, simplifies detecting step, and data processing is simple, then when avoiding two-step method detection
The problem of detection device thermal balance differentia influence detected value precision.Detect the thermostatic control of environment and pair of detection sample and blank sample
Channel is detected simultaneously, and the coupling error of the generations such as variation of ambient temperature, detection device thermal balance difference, operation space and time difference is dropped
To minimum, it is greatly improved detection accuracy and sensitivity.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present application, and the application's shows
Meaning property embodiment and its explanation are not constituted an undue limitation on the present application for explaining the application.
Fig. 1 is that there is the present invention structure of temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device to show
It is intended to.
Fig. 2 is that the commercialization clenobuterol hydrochloride that experimental example 1 uses tries colloidal gold strip detection figure.
Fig. 3 is the examination criteria curve for the clenobuterol hydrochloride that experimental example 1 obtains.
Attached drawing acceptance of the bid note respectively represents: 1- detects sample colloidal gold strip, 2- blank control sample colloidal gold strip, 3- the
One infrared temperature sensor, the second infrared temperature sensor of 4-, 5- first laser generator, 6- second laser generator, 7- add
Hot device, 8- semiconductor cooling fin, 9- heat-conducting plate, 10- radiator fan, 11- temperature sensor, 12- controller, 13- shell.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
As described in background, existing Opto-thertnal detection is highly prone to the interference of environment temperature and ambient temperature, not only
Testing result inaccuracy, and two-step method detection inspection complex steps, data processing complex are unfavorable for opening for its equipment automatization
Hair.Therefore, the invention proposes a kind of with temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device;It is existing
The present invention is further illustrated in conjunction with the drawings and the specific embodiments.
In the following example, the detection sample colloidal gold strip 1, blank control sample colloidal gold strip 2 are Shenzhen
The commercialization clenobuterol hydrochloride colloidal gold strip of Xiang Yun Chemical Co., Ltd..
The controller 12 is to care for beauty EX3G-43HB-24MR touch screen/PLC all-in-one machine.
First infrared temperature sensor 3, the second infrared temperature sensor 4 are that step core MLX90614ESF infrared
Non-contact measurement sensor.
The first laser generator 5, second laser generator 6 are Sanyo's SANYO 100mW 532nm green laser
Mould group.
The heater 7 is the lucky electric heating sheets along electric heating appliance company.
The semiconductor cooling jacket (semiconductor cooling fin 8, heat-conducting plate 9, radiator fan 10) is that intelligence promise Xinda is digital public
The 12V electronic cooling semiconductor external member of department.
The temperature sensor is the K-type thermocouple of the Xinda Zhi Nuo Digit.
Embodiment 1
As shown in Figure 1, it is a kind of with temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device, including
Shell 13, controller 12, Dual channel detection module and temperature automatically controlled module.The Dual channel detection module is located in shell 13
Portion, the controller 12 and temperature automatically controlled module are mounted on shell 13.
The Dual channel detection module includes: the detection group Opto-thertnal detection component being disposed adjacent and control group Opto-thertnal detection group
Part.
The detection group Opto-thertnal detection component includes: detection sample colloidal gold strip 1, the first infrared temperature sensor 3, the
One laser generator 5, first infrared temperature sensor 3 and first laser generator 5 are arranged at detection sample colloidal gold examination
1 top of paper slip, and the launch window of the induction window of the first infrared temperature sensor 3 and first laser generator 5 is towards inspection
Test sample colloidal gold strip 1, first infrared temperature sensor 3 and first laser generator 5 are connect with controller 12.
The control group Opto-thertnal detection component includes: blank control sample colloidal gold strip 2, the second infrared temperature sensor
4, second laser generator 6, second infrared temperature sensor 4 and second laser generator 6 are arranged at blank control sample
2 top of colloidal gold strip, and the launch window of the induction window and second laser generator 6 of the second infrared temperature sensor 4
Towards blank control sample colloidal gold strip 2, second infrared temperature sensor 4 and second laser generator 6 with control
Device 12 processed connects.
Controller 12 starts first laser generator 5 and second laser generator 6 simultaneously, issues identical specific wavelength and swashs
Illumination is mapped to the colloidal gold detection zone on detection sample colloidal gold strip 1 and blank control sample colloidal gold strip 2, glue therein
Body gold absorbs luminous energy and induces infra-red radiation, and the first infrared temperature sensor 3 and the second infrared temperature sensor 4 are the red of detection zone
External radiation amount is converted to temperature value, and sends controller 12 to.Controller 12 calculates the two difference, and according to temperature difference and sample
Standard curve between concentration determines the sample concentration of detection sample.
The temperature automatically controlled module includes temperature sensor 11, heater 7 and chiller assembly, the temperature sensor 11
Setting inside housings, the heater 7 installation on an interior surface of the housing, the heater 7 and temperature sensor 11 with
Controller 12 connects.
The chiller assembly includes semiconductor cooling fin 8, heat-conducting plate 9, radiator fan 10;The heat-conducting plate 9 is closely pacified
In the window opened up on shell, the semiconductor cooling fin 8 is connect with heat-conducting plate 9, and is located in shell, the radiation air
Fan 10 is located at hull outside, and connect with heat-conducting plate 9, and heat is discharged outside shell;The semiconductor cooling fin 8 and radiator fan
10 connect with controller 12.
The temperature range of temperature and setting that controller 12 is detected according to temperature sensor 11 compares, when detection temperature
When degree is lower than set temperature lower limit, controller 12 controls heater 7 and is powered, 11 real-time monitoring shell of temperature sensor in heating process
Body internal temperature, controller 12 controls the power-off of heater 7 after reaching set temperature range;When detection temperature is higher than in set temperature
In limited time, controller 12 controls semiconductor cooling fin 8 and radiator fan 10 is powered, 11 real-time monitoring of temperature sensor in cooling procedure
Enclosure interior temperature, controller 12 controls semiconductor cooling fin 8 and the power-off of radiator fan 10 after reaching set temperature range.
Experimental example 1
Experiment is tested for the property using Opto-thertnal detection device described in embodiment 1, by the first laser generator 5,
The colloidal gold detection zone in 532nm laser irradiation to colloidal gold strip that second laser generator 6 issues, colloidal gold absorb light
Infra-red radiation can be induced, the amount of infrared radiation of detection zone is converted to single temperature value by infrared temperature sensor, according to detection sample
Standard between colloidal gold strip 1 and the detection temperature gap and sample concentration of blank control sample colloidal gold strip 2 is bent
Line determines the sample concentration of detection sample.In detection process, the Opto-thertnal detection device always will depending on changes in ambient temperature
The temperature of detection zone maintains set temperature (20 ± 1 DEG C), realizes the automatic constant-temperature control of detection device.
Commercialization clenobuterol hydrochloride colloidal gold strip is tested using this detection device, hydrochloric acid gram is commercialized
Lun Teluo vision-based detection figure is as shown in Fig. 2, gained standard curve is as shown in Figure 3.
Experimental example 2
Using disclosed in patent document 201710625736.1, " a kind of colloidal gold strip based on photo-thermal effect detection is fixed
Amount detecting device " is tested, and Test paper is identical with experimental example 1, is also commercialization clenobuterol hydrochloride colloidal gold strip.
After testing, the detection of the binary channels Opto-thertnal detection of the clenobuterol hydrochloride test strips of experimental example 1 is limited to 0.3ng
mL-1, it is significantly better than the 1.6ngmL of the single channel infrared temperature sensor detection limit of experimental example 2-1.And relative to experimental example
The detection of 2 detection method, experimental example 1 can be completed with a step, not needed after detecting sample using two-step method detection, then blank is arranged
The problem of control, it is cumbersome to overcome detecting step well, data processing complex;Meanwhile also eliminating environment temperature and background
The interference of temperature will test precision and improve 5 times or more.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for those skilled in the art
For member, various changes and changes are possible in this application.Within the spirit and principles of this application, it is made it is any modification,
Equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. a kind of with temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device, it is characterised in that: including
Shell, controller, Dual channel detection module and temperature automatically controlled module;The Dual channel detection module is located at enclosure interior, described
Controller and temperature automatically controlled module are mounted on shell;The Dual channel detection module and temperature automatically controlled module connect with controller
It is logical.
The Dual channel detection module includes: the detection group Opto-thertnal detection component and control group Opto-thertnal detection component being disposed adjacent;
The detection group Opto-thertnal detection component includes: detection sample colloidal gold strip, the first infrared temperature sensor, first laser
Generator, first infrared temperature sensor and first laser generator are arranged above detection sample colloidal gold strip,
And first infrared temperature sensor induction window and first laser generator launch window towards detection sample colloidal gold examination
Paper slip, first infrared temperature sensor and first laser generator are connect with controller;
The control group Opto-thertnal detection component includes: blank control sample colloidal gold strip, the second infrared temperature sensor, second
Laser generator, second infrared temperature sensor and second laser generator are arranged at blank control sample colloid gold test paper
Above item, and the induction window of the second infrared temperature sensor and the launch window of second laser generator are towards blank control
Sample colloidal gold strip, the second outer temperature sensor and second laser generator are connect with controller.
2. there is temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device as described in claim 1,
Be characterized in that: the temperature automatically controlled module includes heater assembly, chiller assembly, the heater assembly include heater,
Temperature sensor, temperature controller, on an interior surface of the housing, the temperature sensor is arranged in shell for the heater installation
Inside, the heater and temperature sensor are connect with temperature controller.
3. there is temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device as claimed in claim 2,
Be characterized in that: the temperature automatically controlled module includes temperature sensor, heater and chiller assembly, the temperature sensor setting
Inside housings, on an interior surface of the housing, the heater and temperature sensor connect with controller for the heater installation
It is logical.
4. there is temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device as claimed in claim 2,
Be characterized in that: the chiller assembly includes semiconductor cooling fin, heat-conducting plate, radiator fan;The heat-conducting plate is installed tightly to
In the window opened up on shell, the semiconductor cooling fin is connect with heat-conducting plate, and is located in shell, and the radiator fan is located at
Hull outside, and connect with heat-conducting plate, heat is discharged outside shell, the semiconductor cooling fin and radiator fan and controller connect
It is logical.
5. according to any one of claims 1-4 have temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection
Device, it is characterised in that: the controller is single-chip microcontroller or PLC or PC.
6. according to any one of claims 1-4 have temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection
Device, it is characterised in that: the wavelength that the first laser generator and second laser generator issue is green wavelength, and wavelength is most
Preferably 532nm.
7. according to any one of claims 1-4 have temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection
Device, it is characterised in that: the temperature sensor is thermocouple.
8. according to any one of claims 1-4 have temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection
Device, it is characterised in that: the heater is heating sheet or heating film.
9. there is temperature automatically controlled binary channels portable colloidal gold test paper strip Opto-thertnal detection device as described in claim 1,
Be characterized in that: the first laser generator, second laser generator are respectively relative to detection sample colloidal gold strip, blank pair
Colloidal gold strip is obliquely installed in the same old way.
10. being examined as claim 1-9 is described in any item with temperature automatically controlled binary channels portable colloidal gold test paper strip photo-thermal
It surveys device and is the application in Food Inspection, hospital clinical and animal diseases diagnosis field.
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CN112378857A (en) * | 2020-11-13 | 2021-02-19 | 暨南大学 | Micro-nano fiber grating photo-thermal test strip sensor and detection method thereof |
CN117607058B (en) * | 2023-11-22 | 2024-05-28 | 重庆师范大学 | Near infrared double light source photo-thermal analysis sensing imaging device |
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