CN109541248B - Flow injection reaction tank device and reversing fluid unit for same - Google Patents
Flow injection reaction tank device and reversing fluid unit for same Download PDFInfo
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- CN109541248B CN109541248B CN201811510715.6A CN201811510715A CN109541248B CN 109541248 B CN109541248 B CN 109541248B CN 201811510715 A CN201811510715 A CN 201811510715A CN 109541248 B CN109541248 B CN 109541248B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
- G01N35/085—Flow Injection Analysis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/24—Stationary reactors without moving elements inside
- B01J19/2415—Tubular reactors
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Abstract
The application discloses a flow injection reaction tank device and a reversing fluid unit for the device, which comprises a transfusion device, a first forward reaction tube, at least one flow path unit and a detector which are connected in sequence; the flow path unit includes: the first selector valve, the reverse reaction tube, the second selector valve and the second forward reaction tube are connected in series; the device comprises a first sample ring, wherein two ends of the first sample ring are respectively connected with a first selection valve, two ends of the second sample ring are respectively connected with a second selection valve, the first selection valve is used for switching the flow direction of the sample in the first sample ring when all samples enter the first sample ring, and the second selection valve is used for switching the flow direction of the sample in the second sample ring when all samples enter the second sample ring. The flow direction of the fluid can be switched, so that the reagent and the sample can obtain better mixing effect.
Description
Technical Field
The application relates to the field of flow injectors, in particular to a flow injection reaction tank device and a reversing fluid unit for the device.
Background
The liquid flowing direction of the existing flow injector moves towards one direction, liquid flows in a pipeline, a sample and current flow are dispersed and mixed under the pipe wall effect and the flowing effect of the liquid, the forward pipeline is connected with a detector through an infusion device, but the reaction of the sample and a reaction reagent is insufficient due to the limitation of the total length of the pipeline, the concentration mixing of the sample belt is in an insufficient state, the flow speed precision and the flow speed stability error of a pump always exist, the test result is shown in fig. 4, the method of quantifying the concentration by adopting peak height is adopted, and the reproducibility of the peak height detection result has certain error. The above-described problems are a need in the art for a solution.
Disclosure of Invention
The application aims to provide a flow injection reaction cell device with more fully mixed sample band concentration and a reversing fluid unit for the device.
In order to solve the technical problems, the scheme provided by the application is as follows: a flow injection reaction tank device and a reversing fluid unit for the device comprise an infusion device, a first forward reaction tube, at least one flow path unit and a detector which are connected in sequence;
the flow path unit includes: the first selector valve, the reverse reaction tube, the second selector valve and the second forward reaction tube are connected in series;
the device also comprises a first sample ring, two ends of the first sample ring are respectively connected with the first selection valve,
a second sample ring, two ends of the second sample ring are respectively connected with the second selection valve,
when all samples enter the first sample ring, the first selector valve is used for switching the flow direction of the samples in the first sample ring,
the second selector valve is used to switch the flow direction of the samples in the second sample loop when all the samples enter the second sample loop.
Further is: the sum of the lengths of the first forward reaction tube and all the second forward reaction tubes is equal to the sum of the lengths of all the reverse reaction tubes.
Further is: the first selector valve comprises a sample inlet, a sample outlet, a first reversing port and a second reversing port, wherein the sample inlet is used for sample injection, the sample outlet is used for sample discharge, and the first reversing port and the second reversing port are respectively connected with two ends of the first sample ring;
the first selector valve may be switched between state one and state two:
the first state: the sample inlet is connected with the first reversing port, and the second reversing port is connected with the sample outlet;
the state two: the sample inlet is connected with the second reversing port, and the first reversing port is connected with the sample outlet.
Further is: the second selector valve comprises a sample inlet, a sample outlet, a first reversing port and a second reversing port, the sample inlet is used for sample injection, the sample outlet is used for sample discharge, and the first reversing port and the second reversing port are respectively connected with two ends of the second sample ring;
the first selector valve may be switched between state one and state two:
the first state: the sample inlet is connected with the first reversing port, and the second reversing port is connected with the sample outlet;
the state two: the sample inlet is connected with the second reversing port, and the first reversing port is connected with the sample outlet.
Further is: the number of the flow path units is one.
Further is: the number of the flow path units is two.
Further is: the infusion device comprises a sample channel, a reagent channel and a sample injection pump, wherein the sample channel and the reagent channel are connected with the first flow path unit through the sample injection pump.
Further is: the sample injection pump is a multichannel pump.
The present application further provides a reversing fluid cell for a flow injection reaction cell apparatus comprising: the first selector valve, the reverse reaction tube, the second selector valve and the second forward reaction tube are connected in series;
the device also comprises a first sample ring, two ends of the first sample ring are respectively connected with the first selection valve,
a second sample ring, two ends of the second sample ring are respectively connected with the second selection valve,
when all samples enter the first sample ring, the first selector valve is used for switching the flow direction of the samples in the first sample ring,
the second selector valve is used to switch the flow direction of the samples in the second sample loop when all the samples enter the second sample loop.
The application has the beneficial effects that: the flow direction of the fluid can be switched, so that the reagent and the sample can obtain better mixing effect.
Drawings
Fig. 1 is a schematic structural view of the present application.
Fig. 2 is a schematic flow chart of the present application, wherein a is a schematic flow chart of the sample injection, B is a schematic flow chart of the sample in the first sample loop, and C is a schematic flow chart of the sample in the second sample loop.
FIG. 3 is a schematic representation of a sample strip distribution tested using the present application.
Fig. 4 is a schematic diagram of a sample strip distribution using prior art testing.
The reference numerals in the figures illustrate: the infusion device 1, the first forward reaction tube 2a, the second forward reaction tube 2b, the first selector valve 3a, the second selector valve 3b, the sample inlet 31, the sample outlet 32, the first reversing port 33, the second reversing port 34, the reverse reaction tube 4, the first sample loop 5a, the second sample loop 5b, and the detector 6.
Description of the embodiments
The present application will be further described with reference to the accompanying drawings and specific examples, which are not intended to be limiting, so that those skilled in the art will better understand the application and practice it.
As shown in fig. 1, a flow injection reaction cell device and a reversing fluid cell for use in the device comprises an infusion device 1, a first forward reaction tube 2a, at least one flow path cell and a detector 6 connected in sequence.
The infusion device 1 comprises a sample channel, a reagent channel and a sample injection pump, wherein the sample channel and the reagent channel are connected with the first flow path unit through the sample injection pump, and the sample injection pump can be a multi-channel pump.
Each flow path unit includes: a first selector valve 3a, a reverse reaction tube 4, a second selector valve 3b and a second forward reaction tube 2b connected in series, wherein the first selector valve 3a serves as a start end and the second forward reaction tube 2b serves as a tail end; the first sample ring 5a has both ends connected to the first selector valve 3a, and the second sample ring 5b has both ends connected to the second selector valve 3 b.
As shown in fig. 2 (B), when all the samples enter the first sample ring 5a, the first selector valve 3a is used to switch the flow direction of the samples in the first sample ring 5a,
as shown in fig. 2 (C), when all the samples enter the second sample ring 5b, the second selector valve 3b is used to switch the flow direction of the samples in the second sample ring 5 b.
Specifically, in the present application, the first sample ring 5a and the second sample ring 5b are identical in structure, taking the first sample ring 5a as an example, the first selector valve 3a includes a sample inlet 31, a sample outlet 32, a first reversing port 33 and a second reversing port 34, the sample inlet 31 is used for sample injection, the sample outlet 32 is used for sample discharge, the first reversing port 33 and the second reversing port 34 are respectively connected with two ends of the first sample ring 5a, and it is understood that in the second selector valve 3b, the first reversing port 33 and the second reversing port 34 are respectively connected with two ends of the second sample ring 5 b;
the first selector valve 3a and the second selector valve 3b are each switchable between a state one and a state two:
state one: the sample inlet 31 is connected with the first reversing port 33, and the second reversing port 34 is connected with the sample outlet 32;
state two: the sample inlet 31 is connected to the second diverting port 34 and the first diverting port 33 is connected to the sample outlet 32.
The reversing valve includes a stator and a rotor, which can be switched between different states by rotating the rotor, which can be driven by a motor.
The working principle of the application is that firstly, as shown in fig. 2 (a), a sample and a reagent are injected into a first forward reaction tube 2a through an infusion device 1, at this time, a first selector valve 3a is in a first state, the sample and the reagent positively enter into a first sample ring 5a, when a sample belt completely enters into the first sample ring 5a, as shown in fig. 2 (B), at this time, the first selector valve 3a is switched to a second state, the movement direction of the sample changes, and under the action of the infusion device 1, the sample reversely enters into a reverse reaction tube 4 to complete reversing, at this time, the sample reversely enters into a second selector valve 3B from the reaction tube, as shown in fig. 2 (C), reversing the sample can be realized by switching the second selector valve 3B into the second state, so that the sample can be returned to the forward flow again, at this time, the experiment can be detected by a detector 6, and as the flow direction of the sample changes, the reversed liquid can be fully reacted and mixed under the action of a tube wall, thus the accurate detection result can be obtained as shown in fig. 3.
It should be understood that, in order to further improve the mixing effect, a plurality of repeated flow path units may be provided in the present embodiment, one flow path unit including two selection valves of the first selection valve 3a and the second selection valve 3b and three reaction tubes of the first forward reaction tube 2a, the second forward reaction tube 2b and the reverse reaction tube 5 may be provided, and in another embodiment, two flow path units including four selection valves and five pipelines may be provided, and it should be understood that the number of flow path units may be repeatedly provided in order to improve the mixing effect.
In addition, in order to obtain a better mixing effect, the sum of the lengths of the first forward reaction tube 2a and all the second forward reaction tubes 2b is equal to the sum of the lengths of all the reverse reaction tubes 4, so that the forward flow path and the reverse flow path of the sample are the same, the sample can be more fully reacted after passing through the uniform travel in the reactor, and the center part of the sample strip is in a stable reaction balance zone.
The application can make the sample fully diluted, dispersed and reacted with the actual tube wall effect after reciprocating movement in the reactor tube under the same reaction tube length condition, and the sample band has a section of area with stable chemical equilibrium before entering the detector 6, the signal height of the area has good linear consistency with the concentration of the sample, the error caused by other factors such as unstable flow of the infusion pump is avoided, the stability of the detection signal of the detector 6 is improved very well, and the reproducibility of the analysis result is improved very greatly.
The above-described embodiments are merely preferred embodiments for fully explaining the present application, and the scope of the present application is not limited thereto. Equivalent substitutions and modifications will occur to those skilled in the art based on the present application, and are intended to be within the scope of the present application. The protection scope of the application is subject to the claims.
Claims (7)
1. The flow injection reaction tank device is characterized by comprising a transfusion device, a first forward reaction tube, at least one flow path unit and a detector which are connected in sequence;
the flow path unit includes: the first selector valve, the reverse reaction tube, the second selector valve and the second forward reaction tube are connected in series;
the device also comprises a first sample ring, two ends of the first sample ring are respectively connected with the first selection valve,
and a second sample ring, both ends of which are respectively connected with the second selection valve,
when all samples enter the first sample ring, the first selector valve is used for switching the flow direction of the samples in the first sample ring,
the second selector valve is used for switching the flow direction of the samples in the second sample ring when all the samples enter the second sample ring;
the first selector valve and the second selector valve comprise corresponding sample inlets, sample outlets, a first reversing port and a second reversing port, the sample inlets are used for sample injection, the sample outlets are used for sample discharge, and the first reversing port and the second reversing port are respectively connected with two ends of the first sample ring;
the first selector valve and the second selector valve are switched between a first state and a second state:
the first state: the sample inlet is connected with the first reversing port, and the second reversing port is connected with the sample outlet;
the state two: the sample inlet is connected with the second reversing port, and the first reversing port is connected with the sample outlet.
2. The flow injection reaction cell apparatus of claim 1 wherein the sum of the lengths of the first forward reaction tube and all of the second forward reaction tubes is equal to the sum of the lengths of all of the reverse reaction tubes.
3. The flow injection reaction cell apparatus of claim 1, wherein the flow path unit is one.
4. The flow injection reaction cell apparatus of claim 1, wherein the flow path units are two.
5. The flow injection reaction cell apparatus of claim 1, wherein the infusion apparatus comprises a sample channel, a reagent channel, and a sample injection pump, the sample channel and the reagent channel being connected to the flow path unit by the sample injection pump.
6. The flow injection reaction cell apparatus of claim 5, wherein the sample injection pump is a multichannel pump.
7. A reversing fluid cell for a flow injection reaction cell apparatus, comprising: the first selector valve, the reverse reaction tube, the second selector valve and the second forward reaction tube are connected in series;
the device also comprises a first sample ring, two ends of the first sample ring are respectively connected with the first selection valve,
a second sample ring, two ends of the second sample ring are respectively connected with the second selection valve,
the first selector valve is used for switching the flow direction of the sample in the first sample ring when all the samples enter the first sample ring, and the second selector valve is used for switching the flow direction of the sample in the second sample ring when all the samples enter the second sample ring;
the first selector valve and the second selector valve comprise corresponding sample inlets, sample outlets, a first reversing port and a second reversing port, the sample inlets are used for sample injection, the sample outlets are used for sample discharge, and the first reversing port and the second reversing port are respectively connected with two ends of the first sample ring;
the first selector valve and the second selector valve are switched between a first state and a second state:
the first state: the sample inlet is connected with the first reversing port, and the second reversing port is connected with the sample outlet;
the state two: the sample inlet is connected with the second reversing port, and the first reversing port is connected with the sample outlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811510715.6A CN109541248B (en) | 2018-12-11 | 2018-12-11 | Flow injection reaction tank device and reversing fluid unit for same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811510715.6A CN109541248B (en) | 2018-12-11 | 2018-12-11 | Flow injection reaction tank device and reversing fluid unit for same |
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| Publication Number | Publication Date |
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| CN109541248A CN109541248A (en) | 2019-03-29 |
| CN109541248B true CN109541248B (en) | 2023-09-15 |
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| CN201811510715.6A Active CN109541248B (en) | 2018-12-11 | 2018-12-11 | Flow injection reaction tank device and reversing fluid unit for same |
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| CN116618106B (en) * | 2023-07-21 | 2023-09-26 | 深圳赛陆医疗科技有限公司 | Fluid transportation system with variable flow direction, detection system and fluid transportation method thereof |
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