CN109540885B - pH test paper and preparation method thereof - Google Patents

pH test paper and preparation method thereof Download PDF

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CN109540885B
CN109540885B CN201811455221.2A CN201811455221A CN109540885B CN 109540885 B CN109540885 B CN 109540885B CN 201811455221 A CN201811455221 A CN 201811455221A CN 109540885 B CN109540885 B CN 109540885B
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test paper
glossy privet
privet fruit
solution
preparing
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CN109540885A (en
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康莲薇
任晓慧
李轩
苑小诗
刘建军
刘振环
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HEBEI XINGBANG AGRICULTURE TECHNOLOGY Co.,Ltd.
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Hebei University of Engineering
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G01N21/80—Indicating pH value

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Abstract

The invention discloses a pH test paper and a preparation method thereof, belonging to the technical field of laboratory articles, wherein the pH test paper is prepared by dipping filter paper in a glossy privet fruit extract solution; the method can quickly judge the acidity or alkalinity of the solution, and is simple in preparation method and environment-friendly.

Description

pH test paper and preparation method thereof
Technical Field
The invention relates to pH test paper, in particular to pH test paper prepared from a plant extract solution, and belongs to the technical field of laboratory articles.
Background
The pH test paper is widely applied to detecting the acidity or alkalinity of a reagent in a laboratory. Three indicators of methyl red, bromocresol green and thymol blue are arranged on the pH test paper. Methyl red, bromocresol green, thymol blue and phenolphthalein can change color according to a certain rule in solutions with different pH values. The color change ranges of methyl red from pH4.4 (red) to 6.2 (yellow), bromocresol green from pH3.6 (yellow) to 5.4 (green), and thymol blue from pH6.7 (yellow) to 7.5 (blue). The neutral white test paper is soaked by the mixed indicator of quantitative methyl red, quantitative bromocresol green and quantitative thymol blue, and the dried pH test paper can be used for measuring the pH value of the solution. Methyl red, bromocresol green and thymol blue are all chemical products, and the preparation and the use of the chemical products have certain harm to the environment.
Disclosure of Invention
The technical problem to be solved by the invention is to provide the pH test paper, which can quickly judge the pH value of the solution, and has the advantages of simple preparation method and environmental protection.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a pH test paper is prepared by soaking filter paper in fructus Ligustri Lucidi extract solution.
The technical scheme of the invention is further improved as follows: the pH test paper is strip-shaped and is placed in a test paper box in a tape measure shape, the pH test paper is wrapped by tinfoil, and a sawtooth piece is arranged at an opening of the test paper box.
The technical scheme of the invention is further improved as follows: a rotating shaft is arranged in the test paper box, one end of the pH test paper is fixed on the rotating shaft, and the other end of the pH test paper is arranged outside an opening of the test paper box.
The technical scheme of the invention is further improved as follows: the front side of the test paper box is provided with a standard colorimetric card, and the back side of the test paper box is provided with an instruction.
The technical scheme of the invention is further improved as follows: when the test paper is used, the pH test paper is pulled out of the test paper box, the test paper is torn off by utilizing the sawtooth piece on the test paper box, the test paper is placed into a clean surface dish, two drops of liquid to be tested are dripped, the color of the test paper is compared with that of a standard colorimetric card, and the pH value corresponding to the color block closest to the color of the standard colorimetric card is the pH value of the solution.
The technical scheme of the invention is further improved in that the preparation method of the standard colorimetric card comprises the following steps: preparing a series of standard solutions with the pH value of 1-14 by using hydrochloric acid and sodium hydroxide solution, respectively dripping the standard solutions with the pH value of 1-14 onto pH test paper, respectively recording the corresponding colors when the pH values are 1-14, and correspondingly manufacturing the pH values of 1-14 and the corresponding colors into a standard colorimetric card.
The technical proposal of the invention is further improved in that the preparation method of the pH test paper comprises the following steps:
A. drying the glossy privet fruit peel, and performing ultrasonic extraction by using ethanol to obtain a glossy privet fruit extracting solution;
B. filtering the fructus Ligustri Lucidi extractive solution, concentrating the filtrate, and lyophilizing to obtain fructus Ligustri Lucidi extract;
C. dissolving a certain mass of glossy privet fruit extract in water, and diluting to obtain a glossy privet fruit extract solution;
D. and (3) soaking the filter paper in the glossy privet fruit extract solution, taking out and airing to obtain the pH test paper.
The technical scheme of the invention is further improved as follows: in the step A, the mass fraction of ethanol is 50-70%, the ultrasonic extraction temperature is 40-50 ℃, the ultrasonic extraction time is 40-50 min, and the ultrasonic extraction power is 100-200W.
The technical scheme of the invention is further improved as follows: and C, pre-freezing for 20-30 min when the filtrate in the step B is concentrated to 10-15 mL, and freeze-drying for 24-48 h.
The technical scheme of the invention is further improved as follows: the concentration of the glossy privet fruit extract solution in the step C is 0.19-0.41 mu mol/mL.
Due to the adoption of the technical scheme, the invention has the technical progress that:
the pH test paper provided by the invention can quickly judge the pH value of the solution, and is simple in preparation method and environment-friendly.
The anthocyanin contained in the glossy privet fruit is good in water solubility, the leaching solution of the anthocyanin has obvious color change on a solution with the pH value of 1-14, the color is easy to distinguish, the anthocyanin can be used as a pH test paper and an acid-base indicator, the pH test paper prepared by the glossy privet fruit has the same using effect as the traditional pH test paper, and the preparation and the use of the anthocyanin are more green and environment-friendly compared with the traditional pH test paper. In addition, the privet trees are common green tree species in public places such as campuses and the like, and are wide in source and convenient to obtain.
The pH test paper is manufactured into a tape shape, so that the operation of pH value detection can be effectively simplified, the accuracy of a detection result can be improved, and meanwhile, the pH test paper is arranged in the test paper box, so that the test paper can be prevented from being polluted and wasted by moisture, and the pH test paper is convenient to use in various experimental environments.
Drawings
FIG. 1 is a schematic diagram of a pH indicator strip with overwrap according to the present invention;
FIG. 2 is a schematic diagram of a standard color chart according to the present invention;
wherein, the colors 1-14 correspond to the pH values 1-14 respectively.
Detailed Description
The following are some specific embodiments of the present invention for further detailed description.
A pH test paper is prepared by soaking filter paper in fructus Ligustri Lucidi extract solution. The pH test paper is strip-shaped and is placed in the test paper box in a tape measure shape. A rotating shaft is arranged in the test paper box, one end of the pH test paper is fixed on the rotating shaft, and the other end of the pH test paper is arranged outside an opening of the test paper box. The pH test paper is wrapped by tinfoil, and the opening of the test paper box is provided with a sawtooth sheet. The front side of the test paper box is provided with a standard colorimetric card, and the back side of the test paper box is provided with an instruction.
The preparation method comprises the following steps:
A. drying the glossy privet fruit peel, and performing ultrasonic extraction by using 50-70% by mass of ethanol at the extraction temperature of 40-50 ℃, wherein the extraction time is 40-50 min, and the ultrasonic extraction power is 100-200W to obtain a glossy privet fruit extracting solution;
B. carrying out suction filtration on the glossy privet fruit extracting solution, concentrating the filtrate, pre-freezing for 20-30 min when the filtrate is concentrated to 10-15 mL, and then carrying out freeze-drying for 24-48 h to obtain a glossy privet fruit extract;
C. dissolving a certain mass of the glossy privet fruit extract in water, and diluting to obtain a glossy privet fruit extract solution, wherein the concentration of the glossy privet fruit extract solution is 0.19-0.41 mu mol/mL;
D. and (3) soaking the filter paper in the glossy privet fruit extract solution, taking out and airing to obtain the pH test paper.
When the test paper is used, the pH test paper is pulled out of the test paper box, the test paper is torn off by utilizing the sawtooth piece on the test paper box, the test paper is placed into a clean surface dish, two drops of liquid to be tested are dripped, the color of the test paper is compared with that of a standard colorimetric card, and the pH value corresponding to the color block closest to the color of the standard colorimetric card is the pH value of the solution.
The preparation method of the standard colorimetric card comprises the following steps: preparing a series of standard solutions with the pH value of 1-14 by using hydrochloric acid and sodium hydroxide solution, respectively dripping the standard solutions with the pH value of 1-14 onto pH test paper, respectively recording the corresponding colors when the pH values are 1-14, and correspondingly manufacturing the pH values of 1-14 and the corresponding colors into a standard colorimetric card.
Example 1
A pH test paper is prepared by soaking filter paper in fructus Ligustri Lucidi extract solution. The pH test paper is strip-shaped and is placed in the test paper box in a tape measure shape. A rotating shaft is arranged in the test paper box, one end of the pH test paper is fixed on the rotating shaft, and the other end of the pH test paper is arranged outside an opening of the test paper box. The pH test paper is wrapped by tinfoil, and the opening of the test paper box is provided with a sawtooth sheet. The front side of the test paper box is provided with a standard colorimetric card, and the back side of the test paper box is provided with an instruction.
The preparation method comprises the following steps:
A. drying the glossy privet fruit peel, and performing ultrasonic extraction by using 50% of ethanol by mass at 40 ℃ for 40min with the ultrasonic extraction power of 100W to obtain a glossy privet fruit extracting solution;
B. filtering fructus Ligustri Lucidi extractive solution, concentrating the filtrate, pre-freezing for 20min when the filtrate is concentrated to 10mL, and lyophilizing for 24h to obtain fructus Ligustri Lucidi extract;
C. dissolving a certain mass of glossy privet fruit extract in water, and diluting to obtain a glossy privet fruit extract solution with the concentration of 0.19 mu mol/mL;
D. and (3) soaking the filter paper in the glossy privet fruit extract solution, taking out and airing to obtain the pH test paper.
Examples 2 to 3
Examples 2-3 were prepared according to the same procedure as in example 1, except that the process parameters were selected as shown in Table 1 below.
TABLE 1
Figure BDA0001887600750000051
Comparative examples 1 to 11
Comparative examples 1 to 10 are the same as example 1 in terms of production process steps, except that: the comparative example 1 is ultrasonic extracted by ethanol with the mass fraction of 40 percent; the comparative example 2 is ultrasonic extracted by ethanol with the mass fraction of 80 percent; the extraction temperature of comparative example 3 was 30 ℃; the extraction temperature of comparative example 4 was 60 ℃; the extraction time of comparative example 5 was 30 min; the extraction time of comparative example 6 was 60 min; the ultrasonic extraction power of comparative example 7 was 50W; the ultrasonic extraction power of comparative example 8 was 300W. The concentration of the glossy privet fruit extract solution in the control example 9 is 0.18 mu mol/mL; the concentration of the glossy privet fruit extract solution in the control example 10 is 0.42. mu. mol/mL.
Comparative example 11 was a commercially available pH paper.
Test examples
The results of examining the standard solutions having a pH of 11 using the pH test paper prepared in examples 1 to 3 and comparative examples 1 to 10 and the commercially available pH test paper of comparative example 11 are shown in table 2.
TABLE 2
Figure BDA0001887600750000052
Figure BDA0001887600750000061
According to the detection results in the table 2, the pH value detected by the pH test paper prepared in the embodiments 1 to 3 of the invention is equivalent to the effect of the commercially available pH test paper (comparative example 11), and the detection is more accurate than the detection in the comparison examples 1 to 10.

Claims (6)

1. A preparation method of pH test paper is characterized in that: the pH test paper is prepared by dipping filter paper in a glossy privet fruit extract solution; the preparation method comprises the following steps:
A. drying the glossy privet fruit peel, and performing ultrasonic extraction by using ethanol to obtain a glossy privet fruit extracting solution;
B. filtering the fructus Ligustri Lucidi extractive solution, concentrating the filtrate, and lyophilizing to obtain fructus Ligustri Lucidi extract;
C. dissolving a certain mass of glossy privet fruit extract in water, and diluting to obtain a glossy privet fruit extract solution;
D. soaking filter paper in the glossy privet fruit extract solution, taking out, and airing to obtain pH test paper;
in the step A, the mass fraction of ethanol is 50-70%, the ultrasonic extraction temperature is 40-50 ℃, the ultrasonic extraction time is 40-50 min, and the ultrasonic extraction power is 100-200W;
when the filtrate obtained in the step B is concentrated to 10-15 mL, pre-freezing for 20-30 min, and then freeze-drying for 24-48 h;
the concentration of the glossy privet fruit extract solution in the step C is 0.19-0.41 mu mol/mL.
2. The method for preparing a pH indicator paper according to claim 1, wherein: the pH test paper is strip-shaped and is placed in a test paper box in a tape measure shape, the pH test paper is wrapped by tinfoil, and a sawtooth piece is arranged at an opening of the test paper box.
3. The method for preparing a pH indicator paper according to claim 2, wherein: a rotating shaft is arranged in the test paper box, one end of the pH test paper is fixed on the rotating shaft, and the other end of the pH test paper is arranged outside an opening of the test paper box.
4. The method for preparing a pH test paper according to claim 3, wherein: the front side of the test paper box is provided with a standard colorimetric card, and the back side of the test paper box is provided with an instruction.
5. The method for preparing a pH test paper according to any one of claims 1 to 4, wherein: when the test paper is used, the pH test paper is pulled out of the test paper box, the test paper is torn off by utilizing the sawtooth piece on the test paper box, the test paper is placed into a clean surface dish, two drops of liquid to be tested are dripped, the color of the test paper is compared with that of a standard colorimetric card, and the pH value corresponding to the color block closest to the color of the standard colorimetric card is the pH value of the solution.
6. The method for preparing a pH test paper according to claim 5, wherein the standard colorimetric card is prepared by the following steps: preparing a series of standard solutions with the pH value of 1-14 by using hydrochloric acid and sodium hydroxide solution, respectively dripping the standard solutions with the pH value of 1-14 onto pH test paper, respectively recording the corresponding colors when the pH values are 1-14, and correspondingly manufacturing the pH values of 1-14 and the corresponding colors into a standard colorimetric card.
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