CN101413895B - Test paper for determining sulfur dioxide content in garden stuff and edible fungus fresh-keeping can - Google Patents
Test paper for determining sulfur dioxide content in garden stuff and edible fungus fresh-keeping can Download PDFInfo
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- CN101413895B CN101413895B CN200810079843XA CN200810079843A CN101413895B CN 101413895 B CN101413895 B CN 101413895B CN 200810079843X A CN200810079843X A CN 200810079843XA CN 200810079843 A CN200810079843 A CN 200810079843A CN 101413895 B CN101413895 B CN 101413895B
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Abstract
The invention relates to a test paper and a standard colorimetric card thereof which are developed based on the theory that methylene blue dye fades while meeting sulfite. The test paper and the standard colorimetric card are used for quantitatively testing the sulfur dioxide content in fruit and edible fungi can. The invention is characterized by rapidness, convenience, accuracy, convenient carrying, etc. The cost of the test paper preparation and the testing is low, the raw material is non-toxic and harmless, and the processes of preparation and testing are safe.
Description
Technical field
The present invention relates to a kind of detection test paper, specifically is a kind of test paper that is used for tracer liquid sulphuric dioxide, relates in particular to a kind of test paper that is used for quantitative determination fruits and vegetables and edible fungus fresh-keeping can content of sulfur dioxide.
Background technology
The normal sulphite that adds in the food production, because of it has reductibility, the normal physiological oxidation of microorganism capable of blocking, and can suppress oxidasic vigor in fruit and the edible fungi, prevent oxidase to the destruction of nutritional labeling and the change of color, so be used for bleaching, decolouring, anti-oxidant and anticorrosion.Sulphite toxicity is less, but the human body Excessive Intake then has infringement to stomach and intestine, liver, makes the red blood cell decreased hemoglobin.For this reason in the GB to sulphite in foods content (with SO
2Meter) clearly regulation is arranged.Because the content to sulphite in foods in the GB is to detect SO
2Be foundation, therefore select SO for use in the design
2For detecting index.The determination method of sulphuric dioxide mainly contains para-rosaniline method (GB 5009.34-85. determination method for sulphite in foods [S]), iodimetric titration (GB 5009-1996. food sanitary testing method (physics and chemistry part) [S]), fluorescent spectrometry (Martin M.F.Choi, Fluorimetric optode membrane for sulfide detection[J], Analyst, 1998,123:1631-1634) and (Baoxin Li such as flow injection method, Zhujun Zhang, Manli Wu, Flow-injection chemiluminescencedetermination of sulfite using on-line electrogenerated silver (II) as the oxidant, Analytica Chimica Acta[J], 2001,432:311-316).Para-rosaniline method is adopted by GB, but wherein absorption liquid tetrachloro mercury sodium is harmful, in recent years there is report to substitute tetrachloro mercury sodium and makes sulphuric dioxide (Liu Hanchu in the absorption liquid mensuration atmosphere with triethanolamine (TEA), Deng. triethanolamine absorbs sulphuric dioxide in the pinkish red spectrphotometric method for measuring atmosphere (physical and chemical inspection-chemical fascicle) [M], 1993,29 (1): 33～34); Iodimetric titration sensitivity is lower, and wherein still-process is time-consuming and can cause sulphuric dioxide loss; Fluorescence method and flow injection method highly sensitive, but realize that the cost of portable detection is higher.
Summary of the invention
The object of the present invention is to provide a kind of quick, easy, measure the test paper and the standard color comparison card thereof of sulfur dioxide residual quantity in fruits and vegetables and the edible fungus fresh-keeping can cheaply.
A kind of test paper of measuring content of sulfur dioxide in fruits and vegetables and the edible fungus fresh-keeping can provided by the invention makes by the following method:
1) presses certain specification cutting quantitative filter paper, and place exsiccator to keep dry;
2) polyvinyl alcohol (PVA) (PVA) aqueous solution of preparation 1%-5% concentration;
3) filter paper that step 1) is cut is dipped in step 2) in the polyvinyl alcohol water solution for preparing, take out after 5-15 minute, place baking oven, 90-105 ℃ dry 1-3 hour down;
4) the methyl blue solution of preparation 0.4%-0.8% concentration, and with 0.05%-0.5% sodium bicarbonate adjusting pH value 7.8-8.2;
5) filter paper that step 3) is prepared is dipped in the solution that step 4) prepares, and takes out after 5-15 minute, places baking oven, 90-105 ℃ dry 1-3 hour down, obtain test paper.
A kind of standard color comparison card of measuring content of sulfur dioxide test paper in fruits and vegetables and the edible fungus fresh-keeping can provided by the invention, make by the following method: at first prepare sodium sulfite aqueous solution, the concentration of sodium sulphite is respectively in sulphuric dioxide: 0mg/ml, 0.01mg/ml, 0.02mg/ml, 0.03mg/ml, 0.04mg/ml, 0.05mg/ml; Then drip respectively in the test paper surface for preparing with suction pipe this solution respectively, dry, obtain color by dark blue standard color comparison card to light blue graded with variable concentrations.
Measure the method for content of sulfur dioxide in fruits and vegetables and the edible fungus fresh-keeping can: the sample drop of getting the sodium sulphite that contains unknown concentration is in the above-mentioned test paper surface for preparing, demonstration must color, by comparing, draw the concentration of contained sulphuric dioxide in the sample with standard color comparison card.
Detect principle:
Methyl blue dyestuff in the test paper is met inferior sulfate radical generation nucleophilic addition chemical reaction, and two keys position of sulphur atom attack dye molecule inside in the inferior sulfate radical makes that its inner conjugated structure is destroyed, thereby makes and itself change, and its course of reaction is as follows:
Compared with prior art, the present invention meets the principle of inferior sulfate radical generation shank color according to the blue dyestuff of methyl, the application standard colourimetry, a kind of test paper and the standard color comparison card thereof of development, can be used for the content of sulphuric dioxide in detection by quantitative fruit, vegetables and the canned edible mushroom and fungus, and have characteristics such as quick, easy, accurate, easy to carry.The test paper starting material are nontoxic, make and testing process safety.It is with low cost that test paper is made and detected.
Embodiment
The present invention will be further described below in conjunction with examples of implementation.Embodiment 1-4 modifies composition of raw materials for the standard color comparison card test paper, and embodiment 5-7 is the preparation and the use of colorimetric card and test paper.
Embodiment 1.
1) by 2 * 10cm cutting quantitative filter paper, and put with exsiccator in keep dry tack free.
2) polyvinyl alcohol (PVA) (PVA) aqueous solution of preparation 1% concentration.
3) 1) in the filter paper that cuts be dipped in 2) in the solution for preparing, propose after 10 minutes, be positioned in the baking oven, in 100 ℃ dry 2 hours down.
4) the methyl blue solution of preparation 0.5% concentration, and with 0.1% sodium bicarbonate adjusting pH value to 8.
5) with 3) in the test paper for preparing be dipped in by 4) in the solution for preparing, propose after 10 minutes, be positioned in the baking oven, in 100 ℃ dry 2 hours down, obtain test paper.
Embodiment 2.
Methyl blue 0.5%, polyvinyl alcohol (PVA) 5%, sodium bicarbonate 0.1%, all the other are with embodiment 1.
Embodiment 3.
Methyl blue 0.8%, polyvinyl alcohol (PVA) 5%, sodium bicarbonate 0.1%, all the other are with embodiment 1.
Embodiment 4.
Methyl blue 0.8%, polyvinyl alcohol (PVA) 5%, sodium bicarbonate 0.5%, all the other are with embodiment 1.
Embodiment 5.
Preparing sodium sulfite aqueous solution (concentration of sodium sulphite is in sulphuric dioxide) respectively is: 0mg/ml, 0.01mg/ml, 0.02mg/ml, 0.03mg/ml, 0.04mg/ml, 0.05mg/ml (regulation sulphite residual quantity is in sulphuric dioxide in the GB2760-1996 food additives use production standard, and canned mushroom must not surpass 0.05g/kg).
This solution getting above-mentioned variable concentrations with suction pipe drips respectively in the test paper surface for preparing respectively, dries, and obtains by dark blue standard color comparison card to light blue graded.
Embodiment 6.
Get test paper that embodiment 1 prepares and be used for the detection of edible fungus fresh-keeping can sulphuric dioxide.Get a sample solution and drip in the test paper surface, observation test paper this moment is by the dark blue indigo plant that shoals, and the concentration that the standard color comparison card that comparative examples 5 is made is read sulphuric dioxide is 0.04mg/ml.
Claims (2)
1. a test paper of measuring content of sulfur dioxide in fruits and vegetables and the edible fungus fresh-keeping can is characterized in that, makes by the following method:
1) presses certain specification cutting quantitative filter paper, and place exsiccator to keep dry;
2) polyvinyl alcohol (PVA) (PVA) aqueous solution of preparation 1%-5% concentration;
3) filter paper that step 1) is cut is dipped in step 2) in the polyvinyl alcohol water solution for preparing, take out after 5-15 minute, place baking oven, 90-105 ℃ dry 1-3 hour down;
4) the methyl blue solution of preparation 0.4%-0.8% concentration, and with 0.05%-0.5% sodium bicarbonate adjusting pH value 7.8-8.2;
5) filter paper that step 3) is prepared is dipped in the solution that step 4) prepares, and takes out after 5-15 minute, places baking oven, 90-105 ℃ dry 1-3 hour down, obtain test paper.
2. standard color comparison card of measuring content of sulfur dioxide test paper in fruits and vegetables and the edible fungus fresh-keeping can, it is characterized in that, make by the following method: at first prepare sodium sulfite aqueous solution, the concentration of sodium sulphite is respectively in sulphuric dioxide: 0mg/ml, 0.01mg/ml, 0.02mg/ml, 0.03mg/ml, 0.04mg/ml, 0.05mg/ml; Then drip respectively in by the made test paper surface of getting ready of claim 1 with suction pipe this solution respectively, dry, obtain color by dark blue standard color comparison card to light blue graded with variable concentrations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810079843XA CN101413895B (en) | 2008-11-14 | 2008-11-14 | Test paper for determining sulfur dioxide content in garden stuff and edible fungus fresh-keeping can |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810079843XA CN101413895B (en) | 2008-11-14 | 2008-11-14 | Test paper for determining sulfur dioxide content in garden stuff and edible fungus fresh-keeping can |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101413895A CN101413895A (en) | 2009-04-22 |
| CN101413895B true CN101413895B (en) | 2010-06-09 |
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| CN200810079843XA Expired - Fee Related CN101413895B (en) | 2008-11-14 | 2008-11-14 | Test paper for determining sulfur dioxide content in garden stuff and edible fungus fresh-keeping can |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926247A (en) * | 2014-05-06 | 2014-07-16 | 北京农学院 | Preparation method of sulfur-containing substance detection test paper |
| CN110470664A (en) * | 2019-08-23 | 2019-11-19 | 昆明理工大学 | Quickly determine preparation method of the test to picture ID of residues of organophosphate pesticides content |
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2008
- 2008-11-14 CN CN200810079843XA patent/CN101413895B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 申双龙等.亚甲蓝褪色光度法测定二氧化硫.分析化学28 10.2000,28(10),1317. |
| 申双龙等.亚甲蓝褪色光度法测定二氧化硫.分析化学28 10.2000,28(10),1317. * |
| 申双龙等.过氧化氢-亚甲蓝动力学光度法测定亚硝酸根.分析化学30 11.2002,30(11),1405. |
| 申双龙等.过氧化氢-亚甲蓝动力学光度法测定亚硝酸根.分析化学30 11.2002,30(11),1405. * |
| 黄宝华等.亚甲基蓝和天青A退色分光光度测定抗坏血酸的比较研究.中国食品添加剂 3.1999,(3),25-27. |
| 黄宝华等.亚甲基蓝和天青A退色分光光度测定抗坏血酸的比较研究.中国食品添加剂 3.1999,(3),25-27. * |
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| CN101413895A (en) | 2009-04-22 |
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Granted publication date: 20100609 Termination date: 20121114 |