CN109527351A

CN109527351A - A kind of health-care flour and preparation method thereof, wheaten food

Info

Publication number: CN109527351A
Application number: CN201811363681.2A
Authority: CN
Inventors: 冯敏; 孔淑贞
Original assignee: Chongqing Technology and Business University
Current assignee: Chongqing Technology and Business University
Priority date: 2018-11-16
Filing date: 2018-11-16
Publication date: 2019-03-29

Abstract

The invention belongs to food flour technical fields, a kind of health-care flour and preparation method thereof, wheaten food are disclosed, health-care flour is made of according to mass fraction 1 part -10 parts of pueraria lobata, 1 part~5 parts of Radix Paeoniae Alba, 1 part~5 parts of dendrobium nobile, 1 part~2 parts of purslane, 1 part~2 parts of lotus leaf and 10 parts~20 parts of flour；Preparation method: selected Chinese herb pueraria lobata, Radix Paeoniae Alba, dendrobium nobile, purslane, lotus leaf, cleaning, drying, disinfection are spare；Chinese medicine material is crushed, is sieved for subsequent use；Weigh 1 part -10 parts of pueraria lobata, 1 part~5 parts of Radix Paeoniae Alba, 1 part~5 parts of dendrobium nobile, 1 part~2 parts of purslane, 1 part~2 parts of lotus leaf mixed with 10 parts~20 parts of flour, stir evenly, be made mixture；Mixture is subjected to separating twice, sterilizes, packs to obtain health-care flour.The present invention, which has, to be adjusted blood glucose, adjusts the healthcare functions such as blood lipid, prevention cardiovascular and cerebrovascular disease, and good mouthfeel.

Description

A kind of health-care flour and preparation method thereof, wheaten food

Technical field

The invention belongs to food flour technical fields more particularly to a kind of health-care flour and preparation method thereof, wheaten food.

Background technique

Flour is a kind of Major Foods of people's long-term consumption, and amount is huge." flour " often said refers to wheat flour, i.e., with small Wheat grinds the powder come；Flour is a kind of powder worn by wheat, by the number of protein content in flour, can be divided into height Gluten flour, Plain flour, Self- raising flour and without gluten flour；Flour (wheat flour) is the staple food of north of China most area, is used Food variety made of flour is various, makes a lot of variety, and flavor is totally different.Existing flour is only capable of providing basic nutrition, meets full abdomen It needs, has a single function, do not have health-care effect, can not achieve the function for adjusting blood glucose, adjusting blood lipid, preventing cardiovascular and cerebrovascular disease Energy.Hyperglycemia, hyperlipidemia disease incidence are year after year at ascendant trend after improving for present people's living standard, due to cardiovascular and cerebrovascular disease Lethality caused by disease reaches the whole world first；Thus effectively prevent blood glucose rise from the food of intake, blood lipid is increased and reduced Therefore a series of complication of bring are significant.

Dendrobium candidum DendrobimnofficinaleKimuraet Migo is in the time-honored common preciousness of one kind Medicine is distributed mainly on China Guangxi, peace for orchid family Dendrobium perennial grow nonparasitically upon another plant herbaceous plant also known as admirable in all its details grass, iron sheet orchid The ground such as emblem, Zhejiang, Fujian, Sichuan and Yunnan are used as medicine with fresh or dry stem, there is reinforcing stomach reg fluid, nourishing Yin and clearing heat, moistening lung to stop Cough, improving eyesight salubrity and other effects are yin-nourishing key medicines.

Modern study proves that dendrobium candidum contains polysaccharide, amino acid, alkaloid isoreactivity ingredient, have anti-oxidant, anti-swollen Tumor, adjusts immunity, hypoglycemic and other effects at anti-aging, and the polyoses content highest in all active constituents, structure is complicated, is it The research of the material base of diverse biological activities, Dendrobium officinale polysaccharide component and its level-one, secondary structure is of great significance.

It focuses mostly at present about the research of Dendrobium officinale polysaccharide in different parts Thick many candies content, Thick many candies antioxidant activity Etc..Thick many candies isolate and purify after structure and biological activity research it is less.The present invention carries out dendrobium candidum Thick many candies It isolates and purifies, research institute obtains the monosaccharide composition and structure feature of separating polyose, and investigates the hypoglycemic activity of different separating polyoses.

Radix Paeoniae Alba is the dry root of ranunculaceae plant Chinese herbaceous peony PaeonialactifloraPall., has nourishing blood for regulating menstruation, holds back yin The benefits of hidroschesis, the analgesic of soft liver, suppressing liver-YANG, medicinal history is long.Its monoterpene being rich in and its glycoside, triterpenes, flavonoids, tan The compounds such as matter class, organic acid, polysaccharide are its material bases for playing drug effect.The current extraction in relation to Radix Paeoniae Alba chemical component is ground Study carefully and have been reported that more, but spininess respectively holds oneself to Paeoniflorin and total glucosides of paeony, and on the technological parameters such as extracting method, Extraction solvent By reliable extracting method can not be provided for the thoroughly evaluating of Radix Paeoniae Alba quality.

In conclusion problem of the existing technology is:

Existing flour is only capable of providing basic nutrition, meets full abdomen needs, has a single function, do not have health-care effect, cannot Realize the function of adjusting blood glucose, adjust blood lipid, prevent cardiovascular and cerebrovascular disease.

Traditional uniform design and orthogonal design method is extracted in effective component of paeonia lactiflora, test accuracy is low, test number (TN) is more, Mathematical model prediction good job causes the application in health-care flour limited.

Summary of the invention

In view of the problems of the existing technology, the present invention provides a kind of health-care flours and preparation method thereof, wheaten food.

The invention is realized in this way a kind of health-care flour and preparation method thereof, wheaten food, the health-care flour according to Mass fraction is by 1 part -10 parts of pueraria lobata, 1 part~5 parts of Radix Paeoniae Alba, 1 part~5 parts of dendrobium nobile, 1 part~2 parts of purslane, 1 part~2 parts of lotus leaf It is formed with 10 parts~20 parts of flour.

Another object of the present invention is to provide a kind of health-care flour and preparation method thereof, wheaten food, the health-care noodles The preparation method of powder, comprising the following steps:

Step 1: selected Chinese herb pueraria lobata, Radix Paeoniae Alba, dendrobium nobile, purslane, lotus leaf, cleaning, drying, disinfection are spare；

Step 2: Chinese medicine material is crushed, and is sieved for subsequent use；

Step 3, weigh 1 part -10 parts of pueraria lobata in step 3,1 part~5 parts of Radix Paeoniae Alba, 1 part~5 parts of dendrobium nobile, 1 part of purslane~ 2 parts, 1 part~2 parts of lotus leaf are mixed with 10 parts~20 parts of flour, are stirred evenly, and mixture is made；

Mixture is carried out separating twice by step 4, and sterilization, packaging obtains health-care flour.

Further, in step 2, granularity is less than the granularity equal to flour after the crushing of Chinese herb；

Another object of the present invention is to provide the wheaten food prepared by the health-care flour include health-care powder steamed bun, Health care flour noodle, health-care powder food with filling, health-care powder bread, health-care powder cake；

Further, health-care powder steamed bun, health-care powder bread, health-care powder cake can according to for different from consumer's age, It is fabricated to different shape and taste；

Further, health care flour noodle, from consumer's age, can be fabricated to different taste according to for difference；

Further, health-care powder food with filling includes dumpling, won ton, pie, steamed stuffed bun, roulade etc..

Another object of the present invention is to provide a kind of extracting method of Radix Paeoniae Alba in health-care flour, the Radix Paeoniae Alba is mentioned Taking method includes: to choose suitable parameter level by experiment of single factor, and use the Responds Surface Methodology of Three factors-levels The extraction time of Radix Paeoniae Alba ultrasonic extraction process, concentration of alcohol, liquid-to-solid ratio are analyzed, it is super for obtaining optimal extract process Sound time 20.06min, concentration of alcohol 72.04%, liquid-to-solid ratio 53.38mLg^{- 1}。

Further, the extracting method of the Radix Paeoniae Alba specifically includes: taking radix paeoniae alba decoction pieces to crush, crosses 50 meshes, precision weighs suitable Amount, sets in boiling flask, and the appropriate amount of ethanol of respective concentration, weighed, 40kHz is added, and 150W ultrasonic extraction is taken out and stood to room Temperature, weighed quality of supplying is to get Radix Paeoniae Alba.

Further, in Radix Paeoniae Alba ultrasonic extraction,

On the basis of single factor experiment, the level value of 3 liquid-to-solid ratio, concentration of alcohol and ultrasonic time factors is determined, use Design-Expert8.0.6 software carries out the design of Box-Behnken response surface and data carry out variance analysis and quadratic polynomial Regression fit, regression model P are 0.0032, and model is extremely significant；Losing quasi- item P is 0.2360, not significantly；The coefficient of determination is 0.9677, the models fitting degree is good；Factor A, B, B², C²To significantly affect factor, illustrate on Radix Paeoniae Alba extraction effect influence compared with Greatly；Model is optimized manually, on the basis of original fit equation, removes not significant item BC and A²Afterwards, optimize resulting reality Actual value equation:

R=2.2806 × 10⁷+7.8125×10⁴A+4.7447×10⁸B+1.5284×10⁸C-1.3857 × 10³AB- 1.6157×10³AC-3.1633 × 10⁴B²- 3.0159 × 10⁴C²。

Further, the extraction time with the Responds Surface Methodology of Three factors-levels to Radix Paeoniae Alba ultrasonic extraction process, second Before determining alcohol, liquid-to-solid ratio are analyzed, need to carry out:

Reference substance solution preparation weighs gallic acid, catechin, albiflorin, Paeoniflorin, benzoic acid, 1,2,3,4,6- Five galloyl glucose of O-, benzoylpaeoniflorin and Paeonol reference substance are appropriate, add methanol every 1mL is made to contain respectively and do not eat Sub- acid 15.07 μ g, 5.49 μ g of catechin, 11.79 μ g of albiflorin, 35.15 μ g of Paeoniflorin, benzoic acid 7.87 μ g, 1,2,3, The mixing reference substance of 4,6-O- five galloyl glucose, 7.84 μ g, 3.92 μ g of benzoylpaeoniflorin, 7.94 μ g of Paeonol are molten Liquid shakes up, 4 DEG C of storages, spare.

The UPLC analysis condition of Radix Paeoniae Alba

ACQUITYUPLC HSST3 chromatographic column 2.1mm × 100mm, 1.8 μm；- 0.05% phosphoric acid water of mobile phase acetonitrile (A) Solution (B), gradient: 0~0.5min, 7%~14%A；0.5~3min, 14%A；3~5.5min, 14%~18%A； 5.5~6min, 18%~20%A；6~9min, 20%~22%A；9~9.5min, 22%~40%A；9.5~11min, 40%~50%A；11~11.5min, 50%~100%A；11.5~12min,

100%A；12~12.5min, 100%~7%A；12.5~14min, 7%A.Detection wavelength 230nm；Column temperature 30 ℃；Flow velocity 0.4mLmin^{- 1}；2 μ L of sample volume.

The preliminary screening of Radix Paeoniae Alba ultrasonic method extracting method:

10 parts of radix paeoniae alba decoction pieces powder 0.5g is weighed, is placed in boiling flask, 50% ethyl alcohol 50mL of each addition, weighed quality, Wherein 5 parts of samples difference heating and refluxing extractions 0.5,1,1.5,2,2.5h, another 5 parts of samples difference ultrasonic extraction 10,30,50,70, 90min；Standing is extracted to room temperature, weighed to supply quality, 0.22 μm of filtering with microporous membrane takes subsequent filtrate；It selects in chromatogram 11 shared peaks of S/N > 20 are as evaluation index, and the unit crude drug peak area handled with ultrasonic 30min calculates for 100% The shared peak peak area recovery rate of different disposal sample, is compared；Different Extraction Method is to 2,7, No. 8 peak peak area recovery rates Be affected, on remaining share peak peak area influence it is not significant；It is tentatively pushed away by retention time corresponding with reference substance map Survey: No. 2 peaks are gallic acid peak, and No. 7 peaks are benzoic acid peak, and No. 8 peaks are 1,2,3,4,6-O- five galloyl glucoses Peak.

Another object of the present invention is to provide a kind of method for verifying dendrobium nobile effect of lowering blood sugar in the health-care flour, institutes Stating the method for verifying dendrobium nobile effect of lowering blood sugar includes:

Dendrobium candidum Thick many candies are separated using DEAE- cellulose anion exchange chromatography, Trimethylsilation method is surveyed Determine the monosaccharide composition of separating polyose, the structure of infrared spectrum characterization separating polyose；Using model induced by alloxan diabetes mice mould Influence of the type analysis separating polyose to model mice fasting blood sugar, serum insulin level and glycated serum protein content.

Further, the method for the verifying dendrobium nobile effect of lowering blood sugar specifically includes:

1) extraction of dendrobium nobile Thick many candies DOP；

2) dendrobium nobile Thick many candies (DOP) is isolated and purified；

3) monosaccharide composition analysis of dendrobium candidum separating polyose；

4) IR spectrum scanning of dendrobium candidum separating polyose component；

5) Dendrobium officinale polysaccharide hypoglycemic effect is analyzed: mouse modeling, grouping and administration take 18~22g of weight male 100 small, adaptive feeding 3d selects 10 at random and is only used as Normal group, remaining mouse fasting 12h, free water, Alloxan normal saline solution is injected intraperitoneally under 200mg/kg dosage, establishes diabetes animal model；Fasting 12h, after 72h Tail vein blood measurement fasting blood sugar, blood glucose value >=13mmol/L and continuously appearance " three-many-one-little " symptom, modeling success are small Mouse；

Proportionately mould animal body quality and blood glucose level randomly choose 60 and are divided into 6 in successful 75 mouse of modeling Group, respectively model group, positive controls, DOP140mg/kg group, DO P240mg/kg group, DOP340mg/kg group and DOP4 40mg/k g group；

It is administered by following scheme: control group and pure water 20mL/kg stomach-filling daily after model group modeling 7d；Positive controls are given 200mg/kg melbine is given, polysaccharide group presses the daily gastric infusion of various dose 1 time, continuous 21d, fasting 12h after administration.

In conclusion advantages of the present invention and good effect are as follows:

The health-care flour that the present invention obtains, which has, to be adjusted blood glucose, adjusts the healthcare functions such as blood lipid, prevention cardiovascular and cerebrovascular disease, And good mouthfeel.

Carrot rich in carbohydrate, fat, volatile oil, carrotene, vitamin A, vitamin B1, vitamin B2, anthocyanidin, The nutritional ingredient of the needed by human body such as calcium, iron；And carrot is sweet in flavor, pungent, tepor is nontoxic；Cure mainly lower gas bowl spares, sharp chest diaphragm stomach, Five viscera settling makes us strong food, only does good, not harm.

The medical value of navy bean, kidney bean is very high；Ancient Times in China medicine writings are recorded, sweet taste, have warming the middle warmer and descending qi, sharp intestines The functions such as stomach, kidney-nourishing complement are a kind of nourishing diet good foods；There are also sedations for kidney bean, vomit to treatment cold of insufficiency type hiccup, stomach cold It spits, traumatic injury, cough of wheezing, pain in the loins, neuralgia have certain curative effect；Kidney bean or a kind of hyperkalemia hyponatremia food, are well suited for In heart disease, artery sclerosis, hyperlipidemia and avoid salt edible for patients；Modern medical analysis, kidney bean grain are more containing hemagglutinin etc. Kind globulin.

Pea, sweet and neutral are nontoxic；Pea nutritive value: it is rich in vitamin C in the tender leaf of pea pods and bean seedlings and can divide The enzyme of internal nitrosamine is solved, there can be anti-cancer and cancer-preventing with decomposing nitrosamine；Contained by pea stop wooden fork acid, gibberellin and Plant coagulates the substances such as element, has antibacterial anti-inflammatory, enhances the function of metabolism.

Green soya bean is rich in unsaturated fatty acid and soybean lecithin, has and keeps blood vessel elasticity, brain tonic and prevent fatty liver from being formed Effect；Rich in the anticancer components such as saponin, protease inhibitors, isoflavones, molybdenum, selenium in green soya bean, to prostate cancer, cutaneum carcinoma, The almost all of cancer such as intestinal cancer, cancer of the esophagus has inhibiting effect；Green soya bean is also rich in vitamin in addition to containing protein and fiber A, vitamin C and vitamin K and vitamin B；Green soya bean can provide calcium, phosphorus, potassium, iron, zinc, thiamine and riboflavin.

Kudzu-vine root powder provided by the invention: inducing diaphoresis is brought down a fever, and is promoted the production of body fluid, promoting eruption, Shengyang Zhixie.

Radix Paeoniae Alba: returning liver, spleen, lung three to pass through, calming the liver and relieving pain, nourishing blood for regulating menstruation, astringing YIN to stop sweating；For headache and dizziness, hypochondriac pain, abdomen Bitterly, four limbs contraction pain, blood deficiency chlorosis, irregular menstruation, spontaneous perspiration, night sweat.

Dendrobium nobile: nature and flavor are sweet, slightly cold；Channel tropism returns stomach, kidney channel；Major function reinforcing stomach reg fluid, nourishing Yin and clearing heat.For impairment of yin saliva Thanks to, dry polydipsia, deficiency of food are retched, and abnormal heat, mesh are secretly unknown after being ill.

Purslane: purslane two oxyethylamines, malic acid, glucose, calcium, phosphorus, iron and vitamin E rich in, Hu The nutriments such as radish element, vitamin B, vitamin C.Purslane nutritionally has the characteristics that prominent, its 3 fat of ω- Acid content is higher than people and plant.3 fatty acid of ω-can inhibit human body to reduce serum cholesterol concentration, change the absorption of the solid acid of gallbladder Kind vessel wall elasticity, it is highly advantageous to prevention and cure of cardiovascular disease；Nature and flavor acid, trembles with fear；Major function clearing heat and promoting diuresis, removing pattogenic heat from the blood and toxic material from the body；For Bacillary dysentery, acute gastroenteritis, acute appendicitis, mazoitis, hemorrhoid hemorrhage, leukorrhea；Furuncle swelling toxin, eczema, band are controlled in external application Shape bleb.

Lotus leaf: Nelumbonaceae Nelumbo perennial herb emergent aquactic plant；Mainly there are clearing away summerheat, sending up the lucid yang, the function of dissipating stasis and stanching bleeding Effect；Clearing away summer-heat and eliminating dampness, sending up the lucid yang, cooling blood and hemostasis；Effectiveization rouge alkaloid there are many containing in Nuciferine in lotus leaf tea, can have Effect decomposes intracorporal fat, and powerful excretes.

Dandelion: it is specific clearing heat and detoxicating, the effect of diuresis dissipating bind.Cure mainly acute mastitis, adenolymphitis, scrofula, serious case of furuncle Sore swells, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infections.

Fructus lycii: nature and flavor sweet and neutral；Return liver and kidney channel；Major function is nourishing liver and kidney, benefiting shrewd head；For consumption consumptive loss, waist and knee Ache, dizziness and tinnitus, Heat Diabetes, blood deficiency chlorosis, blurred vision.

Jujube: nature and flavor sweet, warm；Returns spleen, stomach meridian；Major function tonifying middle-Jiao and Qi, nourishing blood and tranquilization；It is out of strength for spleen eating less Loose stool, hysteria of womam.

Dried orange peel: damp retention in middle-jiao, stomach duct and abdomen swollen ruffian, loose stool diarrhea, diet reduction, indigestion, stool diarrhea are used for.

Hawthorn: nature and flavor are sour, sweet, tepor；Returns spleen, stomach, Liver Channel；Major function food digesting stomach fortifying, scattered stasis.For meat product It is stagnant, gastral cavilty turgor, diarrhea dysentery abdominal pain, blood stasis closed, postpartum stasis, confidant sting, hernia pain；Hyperlipidemia.Charred FRUCTUS CRATAEGI helps digestion Promoting effect enhancing.For meat stagnation, dysentery is not well.

The present invention separates dendrobium candidum Thick many candies using DEAE- cellulose anion exchange chromatography, and trimethylsilyl ethers is derivative Change method measures the monosaccharide composition of separating polyose, the structure of infrared spectrum characterization separating polyose.Using model induced by alloxan mouse sugar Disease model research separating polyose is urinated to the shadow of model mice fasting blood sugar, serum insulin level and glycated serum protein content It rings.

Isolated 4 kinds of separating polyoses in dendrobium nobile, the monosaccharide group of content highest separating polyose DOP1 become arabinose, half Lactose, glucose and mannose (molar ratio 3.5: 1.2: 1.5: 1.0).Results of IR and monosaccharide composition research phase Symbol, while showing that 4 kinds of separating polyoses are sulfated polysaccharide.4 kinds of separating polyoses can significantly reduce diabetes after 21d is administered The fasting blood sugar (P < 0.01) of model mice improves serum insulin level (P < 0.05~0.01) and reduces saccharification Serum albumin content (P < 0.01), wherein being acted on DOP1 most strong.

Dendrobium nobile separating polyose has significant hypoglycemic effect.

To advanced optimize Radix Paeoniae Alba extraction process, chemically ingredient stability and operation convenience consider, the experimental selection Ultrasonic method analyzes Radix Paeoniae Alba extraction process.Using the total peak area for sharing peak in liquid chromatogram as index, pass through single factor test Method investigates the influence of ultrasonic time, concentration of alcohol, liquid-to-solid ratio to Radix Paeoniae Alba extraction effect, and with the super of response phase method optimization Radix Paeoniae Alba Sound extraction process.

The experimental results showed that influence maximum of the concentration of alcohol to Radix Paeoniae Alba extraction effect, followed by liquid-to-solid ratio and ultrasonic time. Response phase method optimize Radix Paeoniae Alba ultrasonic extraction process optimum condition are as follows: ultrasonic time 20.06min, concentration of alcohol 72.04%, Liquid-to-solid ratio 53.38mLg^{- 1}；The model predication value that peak total peak area is shared in chromatogram is 2.1608 × 10⁸.It is real by verifying Issue after examination and approval existing: under this optimum extraction condition, the actual value that peak total peak area is shared in chromatogram is 2.1422 × 10⁸, with prediction It is worth close, the two relative deviation is 0.86%, illustrates that the resulting Radix Paeoniae Alba extraction process of response surface optimization is stable, feasible.From Radix Paeoniae Alba Extraction effect completely, sufficiently from the point of view of, which obtains Paeoniflorin extraction side in technique " Radix Paeoniae Alba " contained compared with " Chinese Pharmacopoeia " Method has more advantage, can be used for the exploitation, utilization and quality overall evaluation of health-care flour Radix Paeoniae Alba.

Response surface design is a kind of effectively statistics and optimization method, with traditional uniform design compared with orthogonal design, Response phase method has many advantages, such as that test accuracy is high, test number (TN) is few, mathematical model prediction is good,

And it can reflect the relationship between each factor and response.Therefore the present invention is to ring to share peak total peak area in chromatogram It should be worth, carry out the experimental design of Box-Behnken response surface with Design-Expert software, Radix Paeoniae Alba extraction process be carried out excellent Change, provides reliable method for overall merit Radix Paeoniae Alba quality.

The present invention first passes through experiment of single factor and chooses suitable parameter level, and with the response surface of Three factors-levels point Analysis method investigates the extraction time of Radix Paeoniae Alba ultrasonic extraction process, concentration of alcohol, liquid-to-solid ratio, obtains optimal extract process For ultrasonic time 20.06min, concentration of alcohol 72.04%, liquid-to-solid ratio 53.38mLg^{- 1}, and work is demonstrated by confirmatory experiment The feasibility of skill, and at the process conditions, the extraction effect of Radix Paeoniae Alba chemical component more fully, sufficiently, can be used for Radix Paeoniae Alba matter The overall merit of amount, and foundation is provided for the comprehensive utilization of Radix Paeoniae Alba, exploitation.

Detailed description of the invention

Fig. 1 is the preparation method flow chart of health-care flour provided in an embodiment of the present invention.

Specific embodiment

In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to Limit the present invention.

Application principle of the invention is explained in detail with reference to the accompanying drawing.

Health-care flour provided in an embodiment of the present invention, the health-care flour is according to mass fraction by 8-10 parts of pueraria lobata, Radix Paeoniae Alba 3 ~5 parts, 3~5 parts of dendrobium nobile, 2~6 parts of purslane, 1~2 part of lotus leaf, 2~4 parts of dandelion, 1~3 part of fructus lycii, 1~2 part of jujube, 3~5 parts of dried orange peel, 3~5 parts of hawthorn, 10~16 parts of carrot meal, 8~12 parts of navy bean, 30~50 parts of peameal, green soya bean flour 10 ~20 parts and 200~250 parts of wheat flour compositions.

The present invention will be described in further detail with reference to the accompanying drawing；

As shown in Figure 1, the preparation method of health-care flour provided in an embodiment of the present invention, comprising the following steps:

S101: selected Chinese herb pueraria lobata, Radix Paeoniae Alba, dendrobium nobile, purslane, lotus leaf, dandelion, fructus lycii, jujube, dried orange peel, Hawthorn, cleaning, drying, disinfection are spare；

S102: Chinese medicine material is successively pulverized and sieved using micronizer, obtains Chinese medicine fine powder, spare；

S103: carrot being selected, is cleaned, dries pulverizing acquisition carrot meal, spare；It is successively right using screening machine Navy bean, pea and green soya bean carry out screening and removing impurities, and cleaning, drying crushes, and obtain navy bean powder, peameal and green soya bean flour, spare；

S104: pueraria lobata 8-10 parts, 3~5 parts of Radix Paeoniae Alba, 3~5 parts of dendrobium nobile, purslane 2~6 are successively added into wheat flour Part, 1~2 part of lotus leaf, 2~4 parts of dandelion, 1~3 part of fructus lycii, 1~2 part of jujube, 3~5 parts of dried orange peel, 3~5 parts of hawthorn, Hu Luo Foretell 10~16 parts of powder, 8~12 parts of navy bean, 30~50 parts of peameal, 10~20 parts of green soya bean flour mixed, stir evenly, make Obtain mixture；

S105: mixture is subjected to separating twice, filtering, sterilization pack 15~20kg/ bags, obtain health-care flour.

In step S102, granularity is less than or equal to of flour after the crushing of Chinese herb provided in an embodiment of the present invention Granularity.

The wheaten food of health-care flour provided in an embodiment of the present invention preparation include health-care powder steamed bun, health care flour noodle, Health-care powder food with filling, health-care powder bread, health-care powder cake；

Health-care powder steamed bun provided in an embodiment of the present invention, health-care powder bread, health-care powder cake can according to for it is different from Consumer's age is fabricated to different shape and taste；

Health care flour noodle provided in an embodiment of the present invention, from consumer's age, can be fabricated to different mouths according to for difference Taste；

Health-care powder food with filling provided in an embodiment of the present invention includes dumpling, won ton, pie, steamed stuffed bun, roulade etc..

Below with reference to pharmacology analysis, invention is further explained；

Radix Paeoniae Alba: returning liver, spleen, lung three to pass through, calming the liver and relieving pain, nourishing blood for regulating menstruation, astringing YIN to stop sweating；For headache and dizziness, hypochondriac pain, abdomen Bitterly, four limbs contraction pain, blood deficiency chlorosis, irregular menstruation, spontaneous perspiration, night sweat；

Application principle of the invention is further described combined with specific embodiments below.

Embodiment 1:

(1) health-care flour provided in an embodiment of the present invention, the health-care flour is according to mass fraction by 8 parts of pueraria lobata, Radix Paeoniae Alba 3 parts, 3 parts of dendrobium nobile, 2 parts of purslane, 1 part of lotus leaf, 2 parts of dandelion, 1 part of fructus lycii, 1 part of jujube, 3 parts of dried orange peel, 3 parts of hawthorn, Hu Luo Foretell 10 parts of powder, 8 parts of navy bean, 30 parts of peameal, 10 parts of green soya bean flour and 200 parts of wheat flour compositions.

(2) preparation method of health-care flour provided in an embodiment of the present invention, comprising the following steps:

S104: successively into wheat flour add 8 parts of pueraria lobata, 3 parts of Radix Paeoniae Alba, 3 parts of dendrobium nobile, 2 parts of purslane, 1 part of lotus leaf, 2 parts of dandelion, 1 part of fructus lycii, 1 part of jujube, 3 parts of dried orange peel, 3 parts of hawthorn, 10 parts of carrot meal, 8 parts of navy bean, 30 parts of peameal, 10 parts of green soya bean flour are mixed, and are stirred evenly, and mixture is made；

Embodiment 2:

(1) health-care flour provided in an embodiment of the present invention, the health-care flour is according to mass fraction by 10 parts of pueraria lobata, white 5 parts of Chinese herbaceous peony, 5 parts of dendrobium nobile, 6 parts of purslane, 2 parts of lotus leaf, 4 parts of dandelion, 3 parts of fructus lycii, 2 parts of jujube, 5 parts of dried orange peel, 5 parts of hawthorn, Hu 16 parts of carrot powder, 12 parts of navy bean, 50 parts of peameal, 20 parts of green soya bean flour and 250 parts of wheat flour compositions.

S104: successively into wheat flour add 10 parts of pueraria lobata, 5 parts of Radix Paeoniae Alba, 5 parts of dendrobium nobile, 6 parts of purslane, 2 parts of lotus leaf, 4 parts of dandelion, 3 parts of fructus lycii, 2 parts of jujube, 5 parts of dried orange peel, 5 parts of hawthorn, 16 parts of carrot meal, 12 parts of navy bean, 50 parts of peameal, 20 parts of green soya bean flour are mixed, and are stirred evenly, and mixture is made；

Embodiment 3:

(1) health-care flour provided in an embodiment of the present invention, the health-care flour is according to mass fraction by 9 parts of pueraria lobata, Radix Paeoniae Alba 4 parts, 4 parts of dendrobium nobile, 4 parts of purslane, 1.5 parts of lotus leaf, 3 parts of dandelion, 2 parts of fructus lycii, 1.5 parts of jujube, 4 parts of dried orange peel, 4 parts of hawthorn, 14 parts of carrot meal, 10 parts of navy bean, 40 parts of peameal, 15 parts of green soya bean flour and 225 parts of wheat flour compositions.

S104: 9 parts of pueraria lobata, 4 parts of Radix Paeoniae Alba, 4 parts of dendrobium nobile, 4 parts of purslane, lotus leaf 1.5 are successively added into wheat flour Part, 3 parts of dandelion, 2 parts of fructus lycii, 1.5 parts of jujube, 4 parts of dried orange peel, 4 parts of hawthorn, 14 parts of carrot meal, 10 parts of navy bean, peameal 40 parts, 15 parts of green soya bean flour are mixed, and are stirred evenly, and mixture is made；

Below with reference to Radix Paeoniae Alba extracting method in health-care flour of the invention, the invention will be further described.

To advanced optimize Radix Paeoniae Alba extraction process, chemically ingredient stability and operation convenience consider, the experimental selection Ultrasonic method studies Radix Paeoniae Alba extraction process.Using the total peak area for sharing peak in liquid chromatogram as index, pass through single factor test Method investigates the influence of ultrasonic time, concentration of alcohol, liquid-to-solid ratio to Radix Paeoniae Alba extraction effect, and with the super of response phase method optimization Radix Paeoniae Alba Sound extraction process.

The experimental results showed that influence maximum of the concentration of alcohol to Radix Paeoniae Alba extraction effect, followed by liquid-to-solid ratio and ultrasonic time. Response phase method optimize Radix Paeoniae Alba ultrasonic extraction process optimum condition are as follows: ultrasonic time 20.06min, concentration of alcohol 72.04%, Liquid-to-solid ratio 53.38mLg^{- 1}；The model predication value that peak total peak area is shared in chromatogram is 2.1608 × 10⁸.It is real by verifying Issue after examination and approval existing: under this optimum extraction condition, the actual value that peak total peak area is shared in chromatogram is 2.1422 × 10⁸, with prediction It is worth close, the two relative deviation is 0.86%, illustrates that the resulting Radix Paeoniae Alba extraction process of response surface optimization is stable, feasible.From Radix Paeoniae Alba Extraction effect completely, sufficiently from the point of view of, which obtains Paeoniflorin extraction side in technique " Radix Paeoniae Alba " contained compared with " Chinese Pharmacopoeia " Method has more advantage, can be used for the exploitation, utilization and quality overall evaluation of Radix Paeoniae Alba.

Specifically have:

1 material

Radix paeoniae alba decoction pieces are purchased from market, and acetonitrile, methanol are chromatographically pure (German Merck company)；Phosphoric acid is that analysis is pure (commercially available)； 95% ethyl alcohol is medicinal alcohol (commercially available).Gallic acid (purity 89.9%, lot number 110831-201204), catechin (purity 97.2%, lot number 110877-201203), benzoic acid (purity 100%, lot number

100419-201312), Paeoniflorin (purity 94.9%, lot number 110736-201337), Paeonol (purity 100%, Lot number 110708-200506) reference substance be purchased from market；Albiflorin (purity 98%, lot number 20130625), benzoyl Chinese herbaceous peony Glycosides (purity 98%, lot number 20140419) and 1,2,3,4,6-O- five galloyl glucose (purity 98%, lot numbers 20140511) reference substance is purchased from market.ACQUITYUPLC^TMLiquid chromatograph, including binary solvent management system, sample introduction management System, column management system, Generation of tunable UV detector and Empower3.0 chromatographic work station (Wa-ters company, the U.S.)； KQ3200E type ultrasonic cleaner (market)；XS204 type analysis balance (Met-tlerToledo company, the U.S.)；JJ1000 type Electronic balance (market).

2 methods and result

2.1 reference substance solutions preparation precision weighs gallic acid, catechin, albiflorin, Paeoniflorin, benzoic acid, 1, 2,3,4,6-O- five galloyl glucoses, benzoylpaeoniflorin and Paeonol reference substance are appropriate, add methanol that every 1mL is made The 15.07 μ g containing gallic acid respectively, 5.49 μ g of catechin, 11.79 μ g of albiflorin, 35.15 μ g of Paeoniflorin, benzoic acid 7.87 μ g, 1,2,3,4,6-O- five galloyl glucose, 7.84 μ g, 3.92 μ g of benzoylpaeoniflorin, the mixing of 7.94 μ g of Paeonol Reference substance solution shakes up, 4 DEG C of storages, spare.

The UPLC analysis condition of 2.2 Radix Paeoniae Albas

ACQUITYUPLC HSST3 chromatographic column (2.1mm × 100mm, 1.8 μm)；- 0.05% phosphoric acid of mobile phase acetonitrile (A) Aqueous solution (B), gradient: 0~0.5min, 7%~14%A；0.5~3min, 14%A；3~5.5min, 14%~18% A；5.5~6min, 18%~20%A；6~9min, 20%~22%A；9~9.5min, 22%~40%A；9.5~ 11min, 40%~50%A；11~11.5min, 50%~100%A；11.5~12min,

2.3, the preliminary screening of Radix Paeoniae Alba extracting method:

Precision weighs 10 parts of 0.5g of radix paeoniae alba decoction pieces powder (crossing 50 meshes), is placed in boiling flask, each that 50% ethyl alcohol is added 50mL, weighed quality, wherein 5 parts of samples distinguish heating and refluxing extraction 0.5,1,1.5,2,2.5h, ultrasound mentions another 5 parts of samples respectively Take 10,30,50,70,90min.Standing is extracted to room temperature, weighed to supply quality, 0.22 μm of filtering with microporous membrane takes continuous filter Liquid, sample detection.The list for selecting 11 shared peaks of S/N > 20 in chromatogram as evaluation index, and being handled with ultrasonic 30min Position crude drug peak area is 100%, calculates the shared peak peak area recovery rate of different disposal sample, is compared.Different extraction sides Method is affected to 2,7, No. 8 peak peak area recovery rates, influences on remaining peak area for sharing peak not significant.By with compare Product map corresponds to retention time can initial guess: No. 2 peaks are gallic acid peak, and No. 7 peaks are benzoic acid peak, and No. 8 peaks are 1,2,3, 4,6-O- five galloyl glucose peaks.Since heating reflux method Extracting temperature is higher than ultrasonic method, cause contained in Radix Paeoniae Alba Nutgall acyl tannin more facile hydrolysis generates gallic acid or galloyl glucose；The benzoic acid ester bond of monoterpene glycosides substance is more Easy fracture and generate benzoic acid.Therefore, gallic acid, benzoic acid and 1 in heating reflux method, 2,3,4,6-O- five galloyls The peak area recovery rate of glucose is higher than ultrasonic method.It is super to comprehensively consider factors, the selections such as chemical composition stability, operation convenience Sound method is the extracting method of Radix Paeoniae Alba.

The process flow of 2.4 Radix Paeoniae Alba ultrasonic extractions

It takes radix paeoniae alba decoction pieces to crush, crosses 50 meshes, precision is weighed in right amount, set in boiling flask, and the ethyl alcohol of respective concentration is added In right amount, weighed, ultrasonic extraction (40kHz, 150W) is taken out and is stood to room temperature, it is weighed supply quality to get.

The single factor experiment of 2.5 Radix Paeoniae Alba ultrasonic extraction process parameters

Using 11 of S/N > 20 in chromatogram shared peak total peak areas as evaluation index, different ultrasounds is investigated respectively and is mentioned Take the time (10,20,30,40,50min), concentration of alcohol (20%, 35%, 50%, 65%, 80%) and liquid-to-solid ratio (50: 1, 100: 1,250: 1,500: 1,800: 1mLg^{- 1}) influence to Radix Paeoniae Alba extraction efficiency.

2.5.1 the fixed liquid-to-solid ratio of influence of the ultrasonic extraction time to extraction effect is 100: 1 (mLg^{- 1}), concentration of alcohol It is 50%, selection ultrasound 10,20,30,40,50min is tested.With the extension of ultrasonic time, share peak total peak area by It is cumulative big；But after ultrasound 20min, shared peak total peak area tends to be steady, and extraction tends to be complete.To prevent ultrasonic time too long, Extracting temperature is caused to increase, effective component is degraded in Radix Paeoniae Alba, therefore selects 20min for optimal ultrasonic time.

2.5.2 the fixed liquid-to-solid ratio of influence of the concentration of alcohol to extraction effect is 100: 1 (mLg^{- 1}), ultrasonic time is 20min selects 20%, 35%, 50%, 65%, and 80% concentration of alcohol extracts test to Radix Paeoniae Alba, when concentration of alcohol is 65% When, the total peak area at shared peak reaches peak value；When concentration of alcohol is 80%, extraction effect is weakened again, therefore selects 65% For optimal concentration of alcohol.

2.5.3 the fixed ultrasonic time of influence of the liquid-to-solid ratio to extraction effect is 20min, and concentration of alcohol 50% selects liquid Expect ratio 50: 1,100: 1,250: 1,500: 1,800: 1 (mLg^{- 1}) tested, with the increase of liquid-to-solid ratio, share the total of peak Peak area gradually increases, because Extraction solvent volume constantly increases, the contact surface of medicinal powder and solvent molecule also constantly increases, Extracted amount is caused to be increasing；When liquid-to-solid ratio is greater than 100: 1 (mLg^{- 1}), the growth trend of shared peak total peak area slows down, and extracts Tend to be complete；But in view of being significantly increased for liquid-to-solid ratio may generate certain influence to extracting solution pH, make in Radix Paeoniae Alba to pH value The content of material such as sensitive monoterpene glycosides, aromatic acid change, therefore select 100: 1 (mLg^{- 1}) it is more excellent liquid-to-solid ratio.

2.6 response phase methods optimize the test of Radix Paeoniae Alba ultrasonic extraction process:

On the basis of single factor experiment, the level value of 3 liquid-to-solid ratio, concentration of alcohol and ultrasonic time factors is determined, use Design-Expert8.0.6 software carries out the design of Box-Behnken response surface and data analysis.Specific experiment factor and each water Level values see the table below.

Response surface experiments factor and level

According to the Combination Design principle of Box-Behnken, using 3 factor 3 of De-sign-Expert8.0.6 software design Level is tested for 15 totally, wherein central point 3 repeated experiments；The total peak area at peak is shared with S/N > 20 in chromatogram as response It is worth (R), specific experiment design scheme and result following table.

Response surface experimental result

The experimental design and results of response sur- face analysis

Variance analysis and quadratic polynomial regression fit are carried out to experimental data using Design-Expert8.0.6 software, Regression model P is 0.0032, illustrates that model is extremely significant；Losing quasi- item P is 0.2360, not significantly；The coefficient of determination is 0.9677, explanation The models fitting degree is good；Factor A, B, B², C²To significantly affect factor, illustrate to be affected to Radix Paeoniae Alba extraction effect.For letter Change equation, model is optimized manually, on the basis of original fit equation, removes not significant item BC and A²Afterwards, variance analysis As a result it see the table below, optimize resulting actual value equation:

R=2.2806 × 10⁷+7.8125×10⁴A+4.7447×10⁸B+1.5284×10⁸C-1.3857 × 10³AB

- 1.6157 × 10³AC-3.1633 × 10⁴B²- 3.0159 × 10⁴C²。

Analysis of variance table after optimization

Response surface curved surface can more intuitively reflect each factor and influence of the reciprocation of factor to response two-by-two.Radix Paeoniae Alba In the contour map and response surface 3D map of ultrasonic extraction, concentration of alcohol influences maximum, followed by liquid material to Radix Paeoniae Alba extraction effect Than influence of the ultrasonic time to Radix Paeoniae Alba extraction effect is minimum.Liquid-to-solid ratio and the reciprocation of concentration of alcohol are to Radix Paeoniae Alba extraction effect It is affected, is secondly the reciprocation of liquid-to-solid ratio and ultrasonic time, the reciprocation of concentration of alcohol and ultrasonic time is least aobvious It writes.

With the resulting Radix Paeoniae Alba ultrasonic extraction optimised process of Design-Expert8.0.6 software optimization are as follows: liquid-to-solid ratio 53.38mLg-¹, concentration of alcohol 72.04%, ultrasonic time 20.06min, with this condition share peak total peak area prediction Value is 2.1608 × 10⁸。

2.7 verification tests are the reliability of auth response face method acquired results, are tested the process conditions of model optimization Card.In view of the feasibility and convenience of experiment, technological parameter: solid-liquid ratio 53mLg- is selected¹, concentration of alcohol 72%, ultrasound Time 20min carries out experiment 3 times, illustrates equation and the fitting of actual experimental situation preferably, the process of optimization, feasible.

Compare respectively the mentioning by contained content assaying method and Optimized Extraction Process of Radix Paeoniae Alba in " Chinese Pharmacopoeia " (version in 2010) Take effect.By CP method (liquid-to-solid ratio 500mLg-¹, concentration of alcohol 50%, ultrasonic time 30min) prepare test solution 3 Part, sample detection records chromatogram, is compared with confirmatory experiment result.

As a result, it has been found that: it is high compared with CP method by 10.84% that response phase method optimizes the resulting shared peak total peak area of technique；If with Chinese herbaceous peony The peak area of medicine glycosides is evaluation index, the resulting Paeoniflorin peak area of response surface optimization technique low compared with CP method 1.30%, without aobvious Write difference, and liquid material is bigger in CP method, causes each ingredient response in extracting solution relatively low, in chromatogram peak number compared with It is few.Therefore, from the point of view of Radix Paeoniae Alba quality thoroughly evaluating, response phase method optimizes resulting Radix Paeoniae Alba ultrasonic extraction process and mentions compared with pharmacopeia Follow the example of more advantage.

Verification test

Since response phase method is to obtain corresponding point on the basis of experiment of single factor, and it is complete from these points to obtain one Whole, continuous face, then optimal value is obtained in this face.Therefore, in response surface whole design, variable parameter in experiment of single factor Selection seem especially crucial.The lower listed method of version " Chinese Pharmacopoeia " Radix Paeoniae Alba in 2010 is only used for the content detection of Paeoniflorin, But reference value with higher, therefore 3 determined extraction time, concentration of alcohol, liquid-to-solid ratio techniques in this research experiment of single factor The range of variable covers the technological parameter in official method, and expands the selection of technological parameter on the basis of official method Section, to obtain more preferably Radix Paeoniae Alba extraction process.The monoterpene glycosides compounds such as contained Paeoniflorin and some facile hydrolysis in Radix Paeoniae Alba Tannins are more sensitive to temperature, and stability is poor at high temperature, the result of this and extracting method primary dcreening operation in this experiment It is consistent.And to have many advantages, such as extraction efficiency high, easy to operate, energy saving for ultrasonic method, therefore can be used as preferably Radix Paeoniae Alba extracting method.

There is the experimental data for adjusting blood glucose to make to the present invention below with reference to dendrobium nobile in health-care flour of the invention further Description.

It 1, is polysaccharide type and its function of blood sugar reduction in analysis dendrobium nobile.

Method:

Dendrobium candidum Thick many candies are separated using DEAE- cellulose anion exchange chromatography, Trimethylsilation method is surveyed Determine the monosaccharide composition of separating polyose, the structure of infrared spectrum characterization separating polyose.Using model induced by alloxan diabetes mice mould Influence of the type research separating polyose to model mice fasting blood sugar, serum insulin level and glycated serum protein content.

As a result:

Conclusion:

Dendrobium nobile separating polyose has significant hypoglycemic effect.

Specifically:

1,1 material

Dendrobium officinale powder (commercially available)；Trifluoroacetic acid, anhydrous pyridine, D-Fructose, D- xylose, D- galactolipin, L- sandlwood Sugar, L-arabinose (commercially available)；Phenol, D-Glucose, D-MANNOSE (commercially available)；Alloxan, LiClO₄(commercially available)； Regisil reagent (Regis company)；Melbine (commercially available)；Glycated serum protein measures reagent, insulin assay kit (commercially available)；Pure water (purifying of Milli-pore system), other reagents are that analysis is pure.

1.2 animal

SPF grades of ICR mouse (male, 18~22g), credit number: SCXK (Shanghai).2012-0002, quality certification number: 2015000501747。

2 methods and result

The extraction of 2.1 dendrobium candidum Thick many candies (DOP)

Process flow: dendrobium officinale powder → degreasing decoloring element → vacuum drying → hot water extraction → supernatant concentration → alcohol precipitation Shallow lake → freeze-drying → dendrobium candidum Thick many candies → de- albumen → DEAE- cellulose column separation → concentration → ultrafiltration → freeze-drying → separation Polysaccharide.

Degreasing decoloring element: dendrobium officinale powder adds dehydrated alcohol by liquid-to-solid ratio 5: 1, refluxing extraction 3 times, each 1h, filters Filtrate is abandoned, 75 DEG C of filter cake vacuum drying remove fat-soluble pigment and small molecular weight impurity in raw material powder.

Dendrobium candidum Thick many candies (DOP) extract and assay: dendrobe powder after weighing a certain amount of degreasing decoloring element in In 50mL centrifuge tube, by liquid-to-solid ratio 30: 1 add pure water, in thermostat water bath at 80 DEG C extract 2h after centrifuge the extract (4000r/min, 15min),

Gained precipitating continues to extract 3 times and combined extract, and rotation is concentrated by evaporation to original volume half at 75 DEG C, adds 5 The dehydrated alcohol precipitating of amount, 4 DEG C of placement 12h, centrifugation (4000r/min, 15min) are lyophilized afterwards again, and Sevag method takes off albumen and obtains iron Skin dendrobium nobile Thick many candies (DOP).Polyoses content after taking off albumen is measured using phend-sulphuric acid, by Dendrobium officinale polysaccharide yield=iron Skin dendrobium nobile Thick many candies quality/dendrobium officinale powder quality × 100%, calculating yield is 18.56%.

2.2 dendrobium candidum Thick many candies (DOP's) isolates and purifies:

The separation of DEAE- cellulose column: -52 column of DEAE- cellulose (Whatman company) of 25mm × 500mm is utilized Separate Thick many candies.It weighs 0.1g Thick many candies to be dissolved in 10mL pure water, utilizes the NaCl solution of 0,0.1,0.2,0.3 and 0.5mol/L With the flow rate gradient of 1.0mL/min, test tube collects eluent, every pipe 10mL, and each gradient collects 10 pipes, phenolsulfuric acid Method colour developing, monitors eluent absorbance value at 490nm, draws elution curve.According to elution curve set separating polyose component, Using the concentration of Labscale ultrafiltration instrument, desalination, freeze-drying is centrifuged after alcohol precipitation.DOP obtains 4 components through above-mentioned 5 kinds of eluents, It is successively denoted as DOP1, DOP2, DOP3 and DOP4, component is less after the N aCl solution elution of 0.5mol/L is difficult to collect.According to iron Area ratio under skin dendrobium polysaccharide yield (18.56%) and 4 separating polyose component elution curves (17.2: 3.5: 2.1: 1.0), calculate each separating polyose yield is followed successively by 13.41%, 2.743%, 1.64% and 0.7 8%.

The monosaccharide composition analysis separating polyose of 2.3 dendrobium candidum separating polyoses hydrolyzes and balance isomerization: polysaccharide 5mg is taken, 4 mL of trifluoroacetic acid of 2mol/L is added, seals, is transferred in distilling flask the evaporated under reduced pressure at 60 DEG C after hydrolyzing 4h at 120 DEG C (oil pump drains moisture).1mL pyridinium dissolution residue is added, 0.5mL is taken to be placed in scale test tube, 0.2%LiClO4 pyrrole is added Pyridine solution 0.5mL, makes LiClO4 ultimate density 0.1%, and sealing of jumping a queue after mixing is placed isomerization for 24 hours and reached at 60 DEG C To balance.

Separating polyose derivatization: the Regisil reagent of 1mL is added in above-mentioned polysaccharide hydrolysis solution, and vortex mixes, anti-at 60 DEG C 15min is answered to complete the Trimethylsilation of monosaccharide, precision pipettes supernatant pyrrole afterwards for centrifugation (50 00r/min, 10min) Pyridine is dissolved in volumetric flask surely, is drawn 0.5mL and is carried out gas chromatographic analysis.

Monosaccharide standard derivatization: each monosaccharide standard (including glucose, mannose, galactolipin, arabinose, sandlwood Sugar, xylose and fructose) derivatization method is same as above.

GC conditions: Shimadzu GC-2010 gas chromatograph；Chromatographic column: Shimadzu Inert Cap5 capillary chromatographic column (30m×250μm×0.25μm)；Temperature program: 18 0 DEG C of reservation 10min are warming up to 240 DEG C with the speed of 5 DEG C/min, then with The speed of 20 DEG C/min is warming up to 300 DEG C and keeps 10min；Detector: hydrogen ion flame detector (FID)；Injector temperature 260℃；1 μ L of sampling volume；300 DEG C of temperature of detection.

After mixing monosaccharide standard, dendrobium candidum Thick many candies (DOP), each separating polyose component Trimethylsilation Gas chromatogram is Arabic it is found that monosaccharide constitutes and is at maximum up to 31.5% with glucose content in dendrobium candidum Thick many candies (DOP) Sugar, xylose, galactolipin, rhamnose and mannose content are respectively 16.5%, 15%, 13.5%, 12.2% and 11.3%.4 kinds Separating polyose component contains glucose, and wherein the highest separating polyose component DOP1 of content contains arabinose, galactolipin, Portugal Grape sugar and mannose, monosaccharide constitutive molar ratio are 3.5: 1.2: 1.5: 1.0.In the less DOP3 and DOP4 monosaccharide composition of content There are micro fructose.

2.4 dendrobium candidum separating polyose components each separating polyose sample of IR spectrum scanning (DOP1, DOP2, DOP3 and DOP4) with dry KBr powder mixed grinding tabletting, infrared spectrometer scanning wavelength range is 4500~500cm^{- 1}.4 groups The Fourier transform infrared spectroscopy map analysis divided is it is found that 3400cm^{- 1}Occurring biggish absorption peak at left and right is hydroxyl in polysaccharide molecule Base group stretching vibration peak；2900cm^{- 1}Neighbouring stronger absorption peak is-CH in polysaccharide molecule₂C-H stretching vibration peak； 1730cm^{- 1}Neighbouring absorption peak is-COOH group C=O stretching vibration peak；

1640cm^{- 1}Neighbouring absorption peak is C=O stretching vibration peak in-COOR；

1380cm^{- 1}Neighbouring absorption peak is C-O stretching vibration peak in-COOR；

1250cm^{- 1}Neighbouring absorption peak is RSO₂S=O stretching vibration peak in R；

1000~1200cm^{- 1}Neighbouring absorption peak is pyranose ring C-O-C stretching vibration peak；

810cm^{- 1}Neighbouring absorption peak is C-H stretching vibration peak in D- mannopyrane glucosides.Infrared analysis result and monosaccharide The result of component is consistent.

The analysis of 2.5 Dendrobium officinale polysaccharide hypoglycemic effects:

Mouse modeling, grouping and administration take 18~22g of weight male 100 small, and adaptive feeding 3d selects 10 at random It is only used as Normal group, alloxan physiology is injected intraperitoneally under 200m g/kg dosage for remaining mouse fasting 12h, free water Saline solution (Normal group injects isometric physiological saline), establishes diabetes animal model.Fasting 12h, it is quiet in 72h rear molding Arteries and veins blood sampling measurement fasting blood sugar, blood glucose value >=13mmol/L and it is continuous occur " three-many-one-little " symptom (more drinks, more foods, diuresis, Weight mitigate), as modeling success mouse.

Proportionately mould animal body quality and blood glucose level randomly choose 60 and are divided into 6 groups in successful 75 mouse of modeling (n=10), respectively model group, positive controls, DOP1 (40mg/k g) group, DOP2 (40mg/kg) group, DOP3 (40mg/ Kg) group and DOP4 (40mg/kg) group.It is administered by following scheme: control group and pure water 20mL/kg daily after model group modeling 7d Stomach-filling；Positive controls give 200mg/kg melbine, and polysaccharide group presses the daily gastric infusion of various dose 1 time, continuous 21d, Fasting 12h after administration.

Fasting 12h after administration in influence of the Dendrobium officinale polysaccharide to model mice fasting blood glucose level the 7th, 14 and 21 day, tail Venous blood sampling measures fasting blood-glucose.Model group mouse fasting blood glucose level is significantly higher than concurrent control group mouse (P < 0.01), together When in 21d mouse behavior expression meet model needs, diabetes animal model is reliable and stable；Positive control melbine group is given After medicine 21d,

Mouse fasting blood-glucose is decreased significantly (P < 0.01) compared with model group, and blood glucose rate of descent is up to for each group 73.1%；Start to show different degrees of hypoglycemic effect after each polysaccharide group administration 7d, to being administered after 21d and same period model group Comparing has significant difference (P < 0.01), and wherein up to 62.6% in polysaccharide group, secondly 1 group of blood glucose rate of descent of DOP is For DOP2 group, reach 5 5.0%.

The 21st day measurement fasting blood of influence of the Dendrobium officinale polysaccharide to model mice insulin and glycated serum protein level After sugar, mouse plucks eyeball and takes blood, and taken blood 3000r/min at 4 DEG C is centrifuged 1 0min, separates serum, say according to kit Bright book measures serum insulin (INS) and glycated serum protein (GSP) content.The results show that polysaccharide group is aobvious compared with model group Work improves diabetic mice insulin level in serum (P < 0.01), and wherein the effect of DOP1 group is most strong；After 21d is administered, Positive controls and polysaccharide group significantly reduce glycated serum protein (GSP) level (P < in diabetic mice serum 0.01), most strong with positive controls effect, it is secondly DOP1 group.It follows that 4 kinds of dendrobium polysaccharides have drop at this dose The effect of low diabetes model mouse blood sugar level, serum insulin and the horizontal variation tendency of glycated serum protein with it is aforementioned Model mice fasting blood glucose level variation tendency is consistent.

The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.

Claims

1. a kind of health-care flour, which is characterized in that the health-care flour is according to mass fraction by 1 part -10 parts of pueraria lobata, 1 part of Radix Paeoniae Alba ~5 parts, 1 part~5 parts of dendrobium nobile, 1 part~2 parts of purslane, 1 part~2 parts of lotus leaf and 10 parts~20 parts of flour compositions.

2. a kind of preparation method of health-care flour as described in claim 1, which is characterized in that the preparation side of the health-care flour Method, comprising the following steps:

Step 3 weighs 1 part -10 parts of pueraria lobata, 1 part~5 parts of Radix Paeoniae Alba, 1 part~5 parts of dendrobium nobile, 1 part~2 parts, 1 part of lotus leaf of purslane ~2 parts are mixed with 10 parts~20 parts of flour, are stirred evenly, and mixture is made；

3. the preparation method of health-care flour as claimed in claim 2, which is characterized in that in the step 2, Chinese herb Crushing after granularity be less than the granularity equal to flour.

4. a kind of wheaten food of the preparation of the health-care flour as described in claim 1, which is characterized in that the health-care flour preparation Wheaten food includes health-care powder steamed bun, health care flour noodle, the quick-frozen food with filling of health-care powder, health-care powder bread, health-care powder cake.

5. the extracting method of Radix Paeoniae Alba in a kind of health-care flour as described in claim 1, which is characterized in that the extraction side of the Radix Paeoniae Alba Method includes: to choose suitable parameter level by experiment of single factor, and use the Responds Surface Methodology dialogue of Three factors-levels The extraction time of Chinese herbaceous peony ultrasonic extraction process, concentration of alcohol, liquid-to-solid ratio are analyzed, when acquisition optimal extract process is ultrasound Between 20.06min, concentration of alcohol 72.04%, liquid-to-solid ratio 53.38mLg^{- 1}。

6. the extracting method of Radix Paeoniae Alba as claimed in claim 5, which is characterized in that the extracting method of the Radix Paeoniae Alba specifically includes: It takes radix paeoniae alba decoction pieces to crush, crosses 50 meshes, precision is weighed in right amount, set in boiling flask, and the appropriate amount of ethanol of respective concentration is added, and is claimed Fixed, 40kHz, 150W ultrasonic extraction is taken out and is stood to room temperature, and weighed quality of supplying is to get Radix Paeoniae Alba.

7. the extracting method of Radix Paeoniae Alba as claimed in claim 5, which is characterized in that in Radix Paeoniae Alba ultrasonic extraction, in single factor experiment On the basis of, determine the level value of 3 liquid-to-solid ratio, concentration of alcohol and ultrasonic time factors, it is soft with Design-Expert8.0.6 Part carries out the design of Box-Behnken response surface and data carry out variance analysis and quadratic polynomial regression fit, regression model P are 0.0032, model is extremely significant；Losing quasi- item P is 0.2360, not significantly；The coefficient of determination is 0.9677, and the models fitting degree is good； Factor A, B, B², C²To significantly affect factor, illustrate to be affected to Radix Paeoniae Alba extraction effect；Model is optimized manually, in original On the basis of having fit equation, not significant item BC and A is removed²Afterwards, optimize resulting actual value equation:

8. the extracting method of Radix Paeoniae Alba as claimed in claim 5, which is characterized in that with the response surface analysis of Three factors-levels Before method analyzes the extraction time of Radix Paeoniae Alba ultrasonic extraction process, concentration of alcohol, liquid-to-solid ratio, need to carry out:

Reference substance solution preparation

Weigh gallic acid, catechin, albiflorin, Paeoniflorin, benzoic acid, 1,2,3,4,6-O- five galloyl grapes Sugar, benzoylpaeoniflorin and Paeonol reference substance are appropriate, add methanol that every 1mL is made and contain 15.07 μ g of gallic acid, catechin respectively 5.49 μ g, 11.79 μ g of albiflorin, 35.15 μ g of Paeoniflorin, 7.87 μ g of benzoic acid, 1,2,3,4,6-O- five galloyl 7.84 μ g of glucose, 3.92 μ g of benzoylpaeoniflorin, the mixed reference substance solution of 7.94 μ g of Paeonol shake up, 4 DEG C of storages, standby With；

The UPLC analysis condition of Radix Paeoniae Alba

ACQUITYUPLCHSST3 chromatographic column 2.1mm × 100mm, 1.8 μm；- 0.05% phosphate aqueous solution of mobile phase acetonitrile (A) (B), gradient: 0~0.5min, 7%~14%A；0.5~3min, 14%A；3~5.5min, 14%~18%A；5.5~ 6min, 18%~20%A；6~9min, 20%~22%A；9~9.5min, 22%~40%A；9.5~11min, 40%~ 50%A；11~11.5min, 50%~100%A；11.5~12min, 100%A；12~12.5min, 100%~7%A； 12.5~14min, 7%A.Detection wavelength 230nm；30 DEG C of column temperature；Flow velocity 0.4mLmin^{- 1}；2 μ L of sample volume；

0.5g10 parts of radix paeoniae alba decoction pieces powder are weighed, is placed in boiling flask, it is each that 50% ethyl alcohol 50mL, weighed quality, wherein 5 is added Part sample difference heating and refluxing extraction 0.5,1,1.5,2,2.5h, another 5 parts of samples difference ultrasonic extraction 10,30,50,70, 90min；Standing is extracted to room temperature, weighed to supply quality, 0.22 μm of filtering with microporous membrane takes subsequent filtrate；It selects in chromatogram 11 shared peaks of S/N > 20 are as evaluation index, and the unit crude drug peak area handled with ultrasonic 30min calculates for 100% The shared peak peak area recovery rate of different disposal sample, is compared；Different Extraction Method is to 2,7, No. 8 peak peak area recovery rates Be affected, on remaining share peak peak area influence it is not significant；It is tentatively pushed away by retention time corresponding with reference substance map Survey: No. 2 peaks are gallic acid peak, and No. 7 peaks are benzoic acid peak, and No. 8 peaks are 1,2,3,4,6-O- five galloyl glucose peaks.

9. the method for dendrobium nobile effect of lowering blood sugar in health-care flour described in a kind of verifying claim 1, which is characterized in that the verifying The method of dendrobium nobile effect of lowering blood sugar includes:

Dendrobium candidum Thick many candies, the measurement point of Trimethylsilation method are separated using DEAE- cellulose anion exchange chromatography Monosaccharide composition from polysaccharide, the structure of infrared spectrum characterization separating polyose；Using model induced by alloxan diabetes mice model point Analyse influence of the separating polyose to model mice fasting blood sugar, serum insulin level and glycated serum protein content.

10. the method for verifying dendrobium nobile effect of lowering blood sugar as claimed in claim 9, which is characterized in that the verifying dendrobium nobile is hypoglycemic The method of effect specifically includes:

1) extraction of dendrobium nobile Thick many candies DOP；

2) dendrobium nobile Thick many candies DOP is isolated and purified；

4) IR spectrum scanning of dendrobium candidum separating polyose component；

5) Dendrobium officinale polysaccharide hypoglycemic effect is analyzed: mouse modeling, grouping and administration take 18~22g of weight male small by 100 Only, adaptive feeding 3d selects 10 at random and is only used as Normal group, remaining mouse fasting 12h, free water, 200mg/kg Alloxan normal saline solution is injected intraperitoneally under dosage, establishes diabetes animal model；Fasting 12h, tail vein is adopted after 72h Hematometry fasting blood sugar, blood glucose value >=13mmol/L and continuous appearance " three-many-one-little " symptom, modeling success mouse；

Proportionately mould animal body quality and blood glucose level randomly choose 60 and are divided into 6 groups in successful 75 mouse of modeling, point It Wei not model group, positive controls, DOP140mg/kg group, DOP240mg/kg group, DOP340mg/kg group and DOP440mg/kg Group；

It is administered by following scheme: control group and pure water 20mL/kg stomach-filling daily after model group modeling 7d；Positive controls are given 200mg/kg melbine, polysaccharide group press the daily gastric infusion of various dose 1 time, continuous 21d, fasting 12h after administration.