CN109527098A - A kind of lactic acid bacteria formula goat milk powder and preparation method thereof - Google Patents

A kind of lactic acid bacteria formula goat milk powder and preparation method thereof Download PDF

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CN109527098A
CN109527098A CN201811620633.7A CN201811620633A CN109527098A CN 109527098 A CN109527098 A CN 109527098A CN 201811620633 A CN201811620633 A CN 201811620633A CN 109527098 A CN109527098 A CN 109527098A
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lactic acid
acid bacteria
preparation
goat milk
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舒国伟
程方方
李宏
陈合
万红昌
王旭
张萌
郭余粮
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Shaanxi Ya Tai Dairy Industry Co Ltd
Shaanxi University of Science and Technology
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Shaanxi Ya Tai Dairy Industry Co Ltd
Shaanxi University of Science and Technology
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/156—Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1238—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/158—Milk preparations; Milk powder or milk powder preparations containing additives containing vitamins or antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/16—Agglomerating or granulating milk powder; Making instant milk powder; Products obtained thereby

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of lactic acid bacteria formula goat milk powders and preparation method thereof, are made by mass fraction of 1000 parts of fresh sheep creams, 2.5-5 parts of stachyoses, 4-7.5 parts of sodium glutamates, 1-3 parts of xylo-oligosaccharides, 1-3 portions of trehaloses, 2-4 parts of taurines and 0.3-1 parts of vitamin Cs, 0.2-0.5 parts of lactobacillus bulgaricus bacterium powder and 0.2-0.5 parts of streptococcus thermophilus bacterium powder；The pretreated added related additive of fresh sheep cream forms mixture；Mixture is divided into two parts, and portion is spare through processing formation concentrated milk；It is cultivated after another access lactobacillus bulgaricus and streptococcus thermophilus bacterium powder, then adds concentrated milk and other additives again, lactic acid bacteria goat milk powder is obtained by spray drying.The present invention can promote the growth of lactobacillus bulgaricus and streptococcus thermophilus in sheep cream, moreover it is possible to provide protective effect to two bacterium in spray drying, improve its survival rate.It may additionally facilitate the proliferation of beneficial bacteria of intestinal tract and the absorption of minerals and increase intestines peristalsis, the functions such as prevent constipation, to be beneficial to consumer health.

Description

A kind of lactic acid bacteria formula goat milk powder and preparation method thereof
Technical field
The invention belongs to dairy technology field, it is related to a kind of lactic acid bacteria formula goat milk powder and preparation method thereof.
Background technique
Goat milk have protein composition rationally, protein be not easy allergy, goat milk fat it is easy to digest absorb, calcium content is high, The advantages that rich in bioactive substance, epithelial cell growth factor such as identical with breast milk, to maintenance alimentary canal health and skin Skin plays a significant role, and goat milk also contains there are many nucleotide, and cyclic adenosine monophosphate (cAMP) content is 2 times of milk, can promote lactose Digestibility and utilization；Also contain insulin-like growth factor in goat milk, has certain effect to old-aged diabetes tool, here it is " these Careless detailed outline " and " haigoushen " in goat milk on the books " can control and quench one's thirst ", therefore goat milk is to infant, pregnant woman, old man and patient All have good nutrition and health care effect.Sheep milk product is at present with big packaging industry goat milk powder and infant formula goat milk powder Main, product homogeneity is serious, therefore it is necessary to develop sheep cream new product.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, present invention solves the technical problem that being to provide a kind of lactic acid bacteria Formula goat milk powder and preparation method thereof develops a kind of sheep cream new product.
The present invention is to be achieved through the following technical solutions:
A kind of lactic acid bacteria formula goat milk powder, by mass fraction by 1000 parts of fresh sheep creams, 2.5-5 parts of stachyoses, 4-7.5 portions of paddy Propylhomoserin sodium, 1-3 part xylo-oligosaccharide, 1-3 portions of trehaloses, 2-4 parts of taurines and 0.3-1 parts of vitamin Cs, 0.2-0.5 parts of Bao Jiali Sub- lactobacillus bacterium powder and 0.2-0.5 parts of streptococcus thermophilus bacterium powder are made.
A kind of preparation method of lactic acid bacteria formula goat milk powder, comprising the following steps:
Step 1: pressing mass fraction, add 2.5-5 parts of stachyoses, 4-7.5 parts of sodium glutamates, 1- into 1000 parts of fresh sheep creams 3 parts of xylo-oligosaccharides and 1-3 portions of trehaloses, stir evenly to form mixture；Mixture is divided into two parts that proportional region is 2:1-1:1 Mixture；
Step 2: the concentrated milk that solid content is 46-53% is concentrated into after a mixture instantaneous sterilizing, it is spare；
Step 3: another mixture is cooled to 42-46 DEG C through sterilization, is respectively connected to 0.2-0.5 parts of lactobacillus bulgaricus With 0.2-0.5 parts of streptococcus thermophilus bacterium powder, 42 DEG C of constant temperature incubations reach 4.2-4.5 to pH, and spare concentrated milk, temperature drop is added To 20-30 DEG C, 2-4 parts of taurines and 0.3-1 parts of vitamin Cs are added, are uniformly mixed, lactic acid bacteria goat milk is obtained by spray drying Powder.
Further, the spray drying condition of step 3 are as follows: inlet air temperature is 150-170 DEG C, and leaving air temp is 60-80 DEG C, Air pressure is 1-3 atmospheric pressure, and hot-air flow velocity is 0.2-0.4m3/min。
Further, streptococcus thermophilus viable count is 1.2-3.6 × 10 in the resulting lactic acid bacteria goat milk powder of step 38CFU/ G, lactobacillus bulgaricus viable count are 2.2-7.3 × 107CFU/g。
Further, the preparation of lactobacillus bulgaricus are as follows: mentioned by mass volume ratio to the Longstamen Onion Bulb that mass concentration is 10% Addition 0.2-0.4% casein hydrolysate, 0.1-0.3% sodium acetate and 0.2-0.4% Triammonium citrate in liquid is taken to obtain culture medium, Sterilizing cooling；The lactobacillus bulgaricus that 1-3% has been activated is accessed into culture medium by volume, in 37 DEG C of constant temperature incubation 18- For 24 hours, refrigerated centrifuge, which is collected, obtains lactobacillus bulgaricus bacterium mud, 1-3 times of weight of lactobacillus bulgaricus bacterium mud compound of addition Freeze drying protectant is placed in 8-15 DEG C of cold treatment 1-2h, using vacuum freeze drier freeze-drying for 24 hours, obtain viable count be 1.2 × 1011CFU/g-5.0×1011The lactobacillus bulgaricus bacterium powder of CFU/g.
Further, the preparation of Longstamen Onion Bulb extracting solution: taking the Longstamen Onion Bulb of certain mass, by quality and volume proportion (grams per milliliter) The ratio of 1:10 soaks in water 20-30min, is boiled and boiling 30min is maintained to obtain filtrate with 400 mesh filter-cloth filterings； Filter residue is added distilled water heating in quality and the ratio of volume proportion (grams per milliliter) 1:10 again and boils and maintain 20min, filters； Merge filtrate twice and constant volume, obtains the Longstamen Onion Bulb extracting solution that mass concentration is 10%.
Further, the preparation of streptococcus thermophilus bacterium powder are as follows: mentioned by mass volume ratio to the puncture vine that mass concentration is 10% Take addition 1-2% glucose, 0.2-0.4% soy peptone, 0.6-1.0% casein hydrolysate, 0.0015- in liquid 0.0040% sodium glutamate, 0.1-0.2%KH2PO4, pH is adjusted to 6.5-7.0 and obtains culture medium by 0.01-0.05% Tween-80；It presses Volume ratio accesses the streptococcus thermophilus that 3-6% has been activated into culture medium, and in 42 DEG C of constant temperature incubation 9-12h, refrigerated centrifuge is obtained Streptococcus thermophilus bacterium mud, the compound freeze drying protectant of 1-3 times of weight of streptococcus thermophilus bacterium mud of addition, is placed in 8-15 DEG C of cold treatment 1- 2h, for 24 hours using vacuum freeze drier freeze-drying, obtaining viable count is 1.0 × 1011CFU/g-3.6×1011The thermophilic chain of CFU/g Coccus bacterium powder.
Further, it the preparation of puncture vine extracting solution: takes a certain amount of puncture vine to crush, makes its granularity 20-60 mesh；By quality Deionized water is added with the ratio of volume proportion (grams per milliliter) 1:10, boil and keeps boiling 30min, with the filter cloth mistake of 400 mesh Filter；Filter residue is boiled again in quality and the ratio of volume proportion (grams per milliliter) 1:10 addition deionized water and keeps boiling 30min, filtering；Merge filtrate twice and constant volume, obtains the puncture vine extracting solution that mass concentration is 10%.
Compared with prior art, the invention has the following beneficial technical effects:
A kind of lactic acid bacteria formula goat milk powder provided by the invention and preparation method thereof, develops a kind of sheep cream new product；? Add stachyose, sodium glutamate, xylo-oligosaccharide and trehalose in fresh sheep cream, this both can promote in sheep cream lactobacillus bulgaricus and Lactose conversion is lactic acid and the substance that has additional nutrients by the growth of streptococcus thermophilus, moreover it is possible to provide guarantor to two bacterium in spray drying Shield effect, improves its survival rate；In addition, lactic acid bacteria goat milk powder add prebiotics (stachyose and xylo-oligosaccharide), taurine and Vitamin C may additionally facilitate the proliferation of beneficial bacteria of intestinal tract and the absorption of minerals and increase intestines peristalsis, the function such as prevent constipation Can, to be beneficial to consumer health.
Specific embodiment
Specific embodiment is given below.
Embodiment 1
A kind of lactic acid bacteria formula goat milk powder, by mass fraction its by 1000 parts of fresh sheep creams, 5 parts of stachyoses, 6 parts of glutamic acid Sodium, 3 parts of xylo-oligosaccharides, 2.5 portions of trehaloses, 4 parts of taurines and 0.3 part of vitamin C, 0.2 part of lactobacillus bulgaricus bacterium powder and 0.2 part of streptococcus thermophilus bacterium powder is made.
The preparation step of lactic acid bacteria formula goat milk powder are as follows:
First: the preparation of starter bacteria powder
1. prepared by lactobacillus bulgaricus bacterium powder: adding in the Longstamen Onion Bulb extracting solution for being 10% to mass concentration by mass volume ratio Add casein hydrolysate, sodium acetate, Triammonium citrate, making its additive amount is respectively 0.2%, 0.3%, 0.4%, after sterilization and cooling The lactobacillus bulgaricus culture solution activated is accessed by the inoculum concentration of 2% (v/v) afterwards, terminates training in 37 DEG C of constant temperature incubation 20h It supports, refrigerated centrifuge obtains lactobacillus bulgaricus bacterium mud, then adds 1 times of bacterium mud weight of compound freeze drying protectant, then sets In 8 DEG C of cold treatment 1.2h, then it is lyophilized using vacuum freeze drier for 24 hours to get lactobacillus bulgaricus bacterium powder, viable count It is 1.2 × 1011CFU/g。
2. prepared by streptococcus thermophilus bacterium powder: being added in the puncture vine extracting solution for being 10% to mass concentration by mass volume ratio 1% glucose, 0.2% soy peptone, 1% casein hydrolysate, 0.0025% sodium glutamate, 0.10%KH2PO4, 0.03% Tween-80, and pH is adjusted to 6.5, the thermophilus bacteria culture fluid activated is then accessed by the inoculum concentration of 3% (v/v), in 42 DEG C constant temperature incubation 10h, refrigerated centrifuge obtain streptococcus thermophilus bacterium mud, then add 1 times of bacterium mud weight of compound frozen-dried protective Agent is subsequently placed in 10 DEG C of cold treatment 1.5h, then using vacuum freeze drier freeze-drying for 24 hours to get streptococcus thermophilus bacterium powder, Viable count is 2.4 × 1011CFU/g。
Second, lactic acid bacteria goat milk powder preparation: press mass fraction, 1000 parts of fresh fresh goat milk of nonreactive after sterilization pretreatment, 6 parts of stachyoses, 6 parts of sodium glutamates, 3 parts of xylo-oligosaccharides and 2.5 portions of trehaloses are added, stirs evenly, is then divided into two parts, Two parts of proportional regions are 1:1, then will wherein 1 part dropped afterwards through ultra high temperature short time sterilization (130-150 DEG C, heating time 2-8 second) It is 50% that solid content is concentrated under reduced pressure into after temperature；In addition portion is cooled to 45 DEG C through sterilization and is respectively connected to lactobacillus bulgaricus It is 0.2 part by mass fraction additive amount with streptococcus thermophilus bacterium powder；Then 42 DEG C of constant temperature incubations reach 4.5 to pH, then plus Enter the concentrated milk that solid content is 50%, after cooling the temperature to 25 DEG C, 4 parts of taurines and 0.3 part of dimension are added by mass fraction Raw element C passes through spray drying (spray drying condition are as follows: inlet air temperature is 150 DEG C, and leaving air temp is 60 DEG C, air after homogeneous Pressure is 1.5 atmospheric pressure, and hot-air flow velocity is 0.4m3/ min) lactic acid bacteria goat milk powder is obtained, streptococcus thermophilus viable count is 3.0×108CFU/g, lactobacillus bulgaricus viable count are 5.0 × 107CFU/g。
Embodiment 2
A kind of lactic acid bacteria formula goat milk powder, by mass fraction its by 1000 parts of fresh sheep creams, 5 parts of stachyoses, 5 parts of glutamic acid Sodium, 1 part of xylo-oligosaccharide, 2 portions of trehaloses, 2.5 parts of taurines and 0.8 part of vitamin C, 0.3 part of lactobacillus bulgaricus bacterium powder and 0.45 part of streptococcus thermophilus bacterium powder is made.
The preparation step of lactic acid bacteria formula goat milk powder are as follows:
First: the preparation of starter bacteria powder
1. prepared by lactobacillus bulgaricus bacterium powder: adding in the Longstamen Onion Bulb extracting solution for being 10% to mass concentration by mass volume ratio Add casein hydrolysate, sodium acetate, Triammonium citrate, making its additive amount is respectively 0.3%, 0.15%, 0.25%, sterilizing cooling The lactobacillus bulgaricus culture solution activated is accessed by the inoculum concentration of 3% (v/v) afterwards, is terminated in 37 DEG C of constant temperature incubation 20h Culture, refrigerated centrifuge obtain lactobacillus bulgaricus bacterium mud, then add 3 times of bacterium mud weight of compound freeze drying protectant, then 10 DEG C of cold treatment 1.5h are placed in, are then lived using vacuum freeze drier freeze-drying for 24 hours to get lactobacillus bulgaricus bacterium powder Bacterium number is 3.6 × 1011CFU/g。
2. prepared by streptococcus thermophilus bacterium powder: being added in the puncture vine extracting solution for being 10% to mass concentration by mass volume ratio 1.5% glucose, 0.25% soy peptone, 1% casein hydrolysate, 0.0015% sodium glutamate, 0.2%KH2PO4, 0.05% Tween-80, and pH is adjusted to 6.8, the streptococcus thermophilus culture activated is then accessed by the inoculum concentration of 6% (v/v) Liquid, in 42 DEG C of constant temperature incubation 9h, refrigerated centrifuge obtains streptococcus thermophilus bacterium mud, then add 2 times of bacterium mud weight of compound freeze-drying Protective agent is subsequently placed in 12 DEG C of cold treatment 1.5h, is then lyophilized using vacuum freeze drier for 24 hours to get streptococcus thermophilus bacterium Powder, viable count are 2.4 × 1011CFU/g。
Second, lactic acid bacteria goat milk powder preparation: press mass fraction, 1000 parts of fresh fresh goat milk of nonreactive after sterilization pretreatment, 5 parts of stachyoses, 5 parts of sodium glutamates, 1 part of xylo-oligosaccharide and 2 portions of trehaloses are added, are stirred evenly, are then divided into two parts, two Part proportional region is 2:1, then by that wherein few part after ultra high temperature short time sterilization (130-150 DEG C, heating time 2-8 second) The concentrated milk that solid content is 52% is concentrated under reduced pressure into after cooling, that more parts is cooled to 43 DEG C and is respectively connected to protect through sterilization to be added Leah lactobacillus and streptococcus thermophilus bacterium powder are 0.3 part by mass fraction lactobacillus bulgaricus bacterium powder additive amount, thermophilus Bacterium bacterium powder additive amount be 0.45 part, then 42 DEG C of constant temperature incubations to pH reach 4.2, then be added solid content be 52% it is dense Contracting cream after cooling the temperature to 20 DEG C, is added 2.5 parts of taurines and 0.8 part of vitamin C by mass fraction, passes through spray after homogeneous Dry (the spray drying condition are as follows: inlet air temperature is 160 DEG C, and leaving air temp is 70 DEG C, and air pressure is 1 atmospheric pressure, and heat is empty of mist Gas velocity is 0.25m3/ min) lactic acid bacteria goat milk powder is obtained, streptococcus thermophilus viable count is 3.0 × 108CFU/g, bulgarian milk Bacillus viable count is 6.5 × 107CFU/g。
Embodiment 3
A kind of lactic acid bacteria formula goat milk powder, by mass fraction its by 1000 parts of fresh sheep creams, 5 parts of stachyoses, 6 parts of glutamic acid Sodium, 1.8 parts of xylo-oligosaccharides, 1.5 portions of trehaloses, 3.5 parts of taurines and 0.5 part of vitamin C, 0.40 part of lactobacillus bulgaricus bacterium Powder and 0.20 part of streptococcus thermophilus bacterium powder are made.
The preparation step of lactic acid bacteria formula goat milk powder are as follows:
First: the preparation of starter bacteria powder
1. prepared by lactobacillus bulgaricus bacterium powder: adding in the Longstamen Onion Bulb extracting solution for being 10% to mass concentration by mass volume ratio Add casein hydrolysate, sodium acetate, Triammonium citrate, making its additive amount is respectively 0.12%, 0.22%, 0.35%, is sterilized cold But the lactobacillus bulgaricus culture solution activated is accessed after after by the inoculum concentration of 4% (v/v), it is whole in 37 DEG C of constant temperature incubation 18h Only to cultivate, refrigerated centrifuge obtains lactobacillus bulgaricus bacterium mud, 2.5 times of bacterium mud weight of compound freeze drying protectant is then added, 8 DEG C of cold treatment 1h are subsequently placed in, are then lived using vacuum freeze drier freeze-drying for 24 hours to get lactobacillus bulgaricus bacterium powder Bacterium number is 1.8 × 1011CFU/g。
2. prepared by streptococcus thermophilus bacterium powder: being added in the puncture vine extracting solution for being 10% to mass concentration by mass volume ratio 1.8% glucose, 0.30% soy peptone, 0.8% casein hydrolysate, 0.0015% sodium glutamate, 0.2%KH2PO4, 0.05% Tween-80, and pH is adjusted to 6.8, then trained by the streptococcus thermophilus that the inoculum concentration access of 4.5% (v/v) has activated Nutrient solution, in 42 DEG C of constant temperature incubation 12h, refrigerated centrifuge obtains streptococcus thermophilus bacterium mud, and then 1.5 times of bacterium mud weight of addition is compound Freeze drying protectant is subsequently placed in 15 DEG C of cold treatment 2h, is then lyophilized using vacuum freeze drier for 24 hours to get streptococcus thermophilus Bacterium powder, viable count are 3.6 × 1011CFU/g。
Second, lactic acid bacteria goat milk powder preparation: by 1000 parts of the mass fraction fresh fresh goat milk of nonreactive after sterilization pretreatment, add Add 5 parts of stachyoses, 6 parts of sodium glutamates, 1.8 parts of xylo-oligosaccharides and 1.5 portions of trehaloses, stir evenly, be then divided into two parts, Two parts of proportional regions are 1.5:1, then by that wherein few part through ultra high temperature short time sterilization (130-150 DEG C, heating time 2-8 Second) cool down afterwards after to be concentrated under reduced pressure into solid content be 50%, that more parts is cooled to 42 DEG C through sterilization and is respectively connected to Bao Jiali Sub- lactobacillus and streptococcus thermophilus bacterium powder are 0.3 part by mass fraction lactobacillus bulgaricus bacterium powder additive amount, streptococcus thermophilus Bacterium powder additive amount is 0.15 part, and then 42 DEG C of constant temperature incubations to pH reach 4.3, and the concentration that solid content is 50% is then added After cooling the temperature to 25 DEG C, 3.5 parts of taurines and 0.5 part of vitamin C are added by mass fraction, by spraying after homogeneous in cream Dry (spray drying condition are as follows: inlet air temperature is 150 DEG C, and leaving air temp is 65 DEG C, and air pressure is 1.5 atmospheric pressure, and heat is empty Gas velocity is 0.30m3/ min) lactic acid bacteria goat milk powder is obtained, streptococcus thermophilus viable count is 2.7 × 108CFU/g, bulgarian milk Bacillus viable count is 5.4 × 107CFU/g。
Embodiment 4
A kind of lactic acid bacteria formula goat milk powder, by mass fraction its by 1000 parts of fresh sheep creams, 3 parts of stachyoses, 6 parts of glutamic acid paddy Propylhomoserin sodium, 3 parts of xylo-oligosaccharides, 2.5 portions of trehaloses, 4 parts of taurines and 0.75 part of vitamin C, 0.25 part of lactobacillus bulgaricus Bacterium powder and 0.25 part of streptococcus thermophilus bacterium powder are made.
The preparation step of lactic acid bacteria formula goat milk powder are as follows:
First: the preparation of starter bacteria powder
1. lactobacillus bulgaricus bacterium powder prepare: by mass volume ratio to mass concentration be 10% by mass volume ratio to Casein hydrolysate, sodium acetate, Triammonium citrate are added in 10% Longstamen Onion Bulb extracting solution, its additive amount is made to be respectively 0.35%, 0.22%, 0.35%, the lactobacillus bulgaricus culture activated is accessed by the inoculum concentration of 4.5% (v/v) afterwards after sterilization and cooling Liquid terminates culture in 37 DEG C of constant temperature incubations for 24 hours, and refrigerated centrifuge obtains lactobacillus bulgaricus bacterium mud, then adds bacterium mud weight 3 Compound freeze drying protectant again, is subsequently placed in 12 DEG C of cold treatment 2h, is then lyophilized using vacuum freeze drier for 24 hours to get guarantor Add Leah lactobacillus bacterium powder, viable count is 1.25 × 1011CFU/g。
2. prepared by streptococcus thermophilus bacterium powder: being added in the puncture vine extracting solution for being 10% to mass concentration by mass volume ratio 1.2% glucose, 0.30% soy peptone, 0.85% casein hydrolysate, 0.0040% sodium glutamate, 0.20%KH2PO4, 0.03% Tween-80, and pH is adjusted to 6.5, the streptococcus thermophilus culture activated is then accessed by the inoculum concentration of 4% (v/v) Liquid, in 42 DEG C of constant temperature incubation 12h, refrigerated centrifuge obtains streptococcus thermophilus bacterium mud, then adds 3 times of bacterium mud weight of compound freeze-drying Protective agent is subsequently placed in 15 DEG C of cold treatment 2h, then using vacuum freeze drier freeze-drying for 24 hours to get streptococcus thermophilus bacterium powder, Its viable count is 1.42 × 1011CFU/g。
Second, lactic acid bacteria goat milk powder preparation: by 1000 parts of the mass fraction fresh fresh goat milk of nonreactive after sterilization pretreatment, add Add 3 parts of stachyoses, 6 parts of sodium glutamates, 3 parts of xylo-oligosaccharides and 2.5 portions of trehaloses, stir evenly, be then divided into two parts, Will wherein 1 part cool down afterwards through ultra high temperature short time sterilization (130-150 DEG C, heating time 2-8 second) after be concentrated under reduced pressure into solid content and contain The concentrated milk that amount is 52%, another is cooled to 43 DEG C through sterilization and is respectively connected to lactobacillus bulgaricus and streptococcus thermophilus bacterium Powder is 0.25 part by mass fraction lactobacillus bulgaricus and streptococcus thermophilus bacterium powder additive amount；Then 42 DEG C of constant temperature incubations Reach 4.2 to pH, the concentrated milk that solid content is 52% is then added, after cooling the temperature to 20 DEG C, is added by mass fraction 2.5 parts of taurines and 0.8 part of vitamin C pass through spray drying (spray drying condition are as follows: inlet air temperature 170 after homogeneous DEG C, leaving air temp is 80 DEG C, and air pressure is 2.5 atmospheric pressure, and hot-air flow velocity is 0.25m3/ min) obtain lactic acid bacteria goat milk Powder, streptococcus thermophilus viable count are 1.2 × 108CFU/g, lactobacillus bulgaricus viable count are 2.3 × 107CFU/g。
Embodiment 5
A kind of lactic acid bacteria formula goat milk powder, by mass fraction its by 1000 parts of fresh sheep creams, 2.5 parts of stachyoses, 4 parts of glutamic acid Sodium, 1.8 parts of xylo-oligosaccharides, 1 portion of trehalose, 2 parts of taurines and 1 part of vitamin C, 0.5 part of lactobacillus bulgaricus bacterium powder and 0.5 Part streptococcus thermophilus bacterium powder is made.
The preparation step of lactic acid bacteria formula goat milk powder are as follows:
First: the preparation of starter bacteria powder
1. prepared by lactobacillus bulgaricus bacterium powder: adding in the Longstamen Onion Bulb extracting solution for being 10% to mass concentration by mass volume ratio Add casein hydrolysate, sodium acetate, Triammonium citrate, making its additive amount is respectively 0.4%, 0.1%, 0.2%, after sterilization and cooling The lactobacillus bulgaricus culture solution activated is accessed by the inoculum concentration of 4% (v/v) afterwards, terminates training in 37 DEG C of constant temperature incubation 18h It supports, refrigerated centrifuge obtains lactobacillus bulgaricus bacterium mud, then adds 2.5 times of bacterium mud weight of compound freeze drying protectant, then 15 DEG C of cold treatment 2h are placed in, are then lyophilized using vacuum freeze drier for 24 hours to get lactobacillus bulgaricus bacterium powder, viable bacteria Number is 2.1 × 1011CFU/g。
2. prepared by streptococcus thermophilus bacterium powder: being added in the puncture vine extracting solution for being 10% to mass concentration by mass volume ratio 2% glucose, 0.4% soy peptone, 0.6% casein hydrolysate, 0.0015% sodium glutamate, 0.2%KH2PO4, 0.01% Tween-80, and pH is adjusted to 7, the streptococcus thermophilus culture activated is then accessed by the inoculum concentration of 4.5% (v/v) Liquid, in 42 DEG C of constant temperature incubation 12h, refrigerated centrifuge obtains streptococcus thermophilus bacterium mud, then adds 1.5 times of bacterium mud weight of compound jelly Dry protective agent is subsequently placed in 8 DEG C of cold treatment 1.2h, is then lyophilized using vacuum freeze drier for 24 hours to get streptococcus thermophilus bacterium Powder, viable count are 3.8 × 1011CFU/g。
Second, lactic acid bacteria goat milk powder preparation: by 1000 parts of the mass fraction fresh fresh goat milk of nonreactive after sterilization pretreatment, add Add 2.5 parts of stachyoses, 4 parts of sodium glutamates, 1.8 parts of xylo-oligosaccharides and 1 portion of trehalose, stir evenly, be then divided into two parts, Two parts of proportional regions are 1.5:1, then by that wherein few part through ultra high temperature short time sterilization (130-150 DEG C, heating time 2-8 Second) cool down afterwards after to be concentrated under reduced pressure into solid content be 46%, that more parts is cooled to 46 DEG C through sterilization and is respectively connected to Bao Jiali Sub- lactobacillus and streptococcus thermophilus bacterium powder are 0.5 part by mass fraction lactobacillus bulgaricus bacterium powder additive amount, streptococcus thermophilus Bacterium powder additive amount is 0.5 part, and then 42 DEG C of constant temperature incubations to pH reach 4.3, and the concentration that solid content is 46% is then added Cream after cooling the temperature to 30 DEG C, is added 2 parts of taurines and 1 part of vitamin C by mass fraction, passes through spray drying after homogeneous (spray drying condition are as follows: inlet air temperature is 150 DEG C, and leaving air temp is 65 DEG C, and air pressure is 3 atmospheric pressure, hot-air flow velocity For 0.2m3/ min) lactic acid bacteria goat milk powder is obtained, streptococcus thermophilus viable count is 2.9 × 108CFU/g, lactobacillus bulgaricus are living Bacterium number is 5.7 × 107CFU/g。
Embodiment 6
A kind of lactic acid bacteria formula goat milk powder, by mass fraction its by 1000 parts of fresh sheep creams, 3 parts of stachyoses, 7.5 parts of glutamic acid Sodium glutamate, 3 parts of xylo-oligosaccharides, 3 portions of trehaloses, 4 parts of taurines and 0.75 part of vitamin C, 0.25 part of lactobacillus bulgaricus Bacterium powder and 0.25 part of streptococcus thermophilus bacterium powder are made.
The preparation step of lactic acid bacteria formula goat milk powder are as follows:
First: the preparation of starter bacteria powder
1. lactobacillus bulgaricus bacterium powder prepare: by mass volume ratio to mass concentration be 10% by mass volume ratio to Casein hydrolysate, sodium acetate, Triammonium citrate are added in 10% Longstamen Onion Bulb extracting solution, its additive amount is made to be respectively 0.35%, 0.22%, 0.35%, the lactobacillus bulgaricus culture activated is accessed by the inoculum concentration of 4.5% (v/v) afterwards after sterilization and cooling Liquid terminates culture in 37 DEG C of constant temperature incubations for 24 hours, and refrigerated centrifuge obtains lactobacillus bulgaricus bacterium mud, then adds bacterium mud weight 3 Compound freeze drying protectant again, is subsequently placed in 12 DEG C of cold treatment 2h, is then lyophilized using vacuum freeze drier for 24 hours to get guarantor Add Leah lactobacillus bacterium powder, viable count is 1.25 × 1011CFU/g。
2. prepared by streptococcus thermophilus bacterium powder: being added in the puncture vine extracting solution for being 10% to mass concentration by mass volume ratio 1.2% glucose, 0.30% soy peptone, 0.85% casein hydrolysate, 0.0040% sodium glutamate, 0.20%KH2PO4, 0.03% Tween-80, and pH is adjusted to 6.5, the streptococcus thermophilus culture activated is then accessed by the inoculum concentration of 4% (v/v) Liquid, in 42 DEG C of constant temperature incubation 12h, refrigerated centrifuge obtains streptococcus thermophilus bacterium mud, then adds 3 times of bacterium mud weight of compound freeze-drying Protective agent is subsequently placed in 15 DEG C of cold treatment 2h, then using vacuum freeze drier freeze-drying for 24 hours to get streptococcus thermophilus bacterium powder, Its viable count is 1.42 × 1011CFU/g。
Second, lactic acid bacteria goat milk powder preparation: by 1000 parts of the mass fraction fresh fresh goat milk of nonreactive after sterilization pretreatment, add Add 3 parts of stachyoses, 6 parts of sodium glutamates, 3 parts of xylo-oligosaccharides and 2.5 portions of trehaloses, stir evenly, be then divided into two parts, Will wherein 1 part cool down afterwards through ultra high temperature short time sterilization (130-150 DEG C, heating time 2-8 second) after be concentrated under reduced pressure into solid content and contain The concentrated milk that amount is 53%, another is cooled to 43 DEG C through sterilization and is respectively connected to lactobacillus bulgaricus and streptococcus thermophilus bacterium Powder is 0.25 part by mass fraction lactobacillus bulgaricus and streptococcus thermophilus bacterium powder additive amount；Then 42 DEG C of constant temperature incubations Reach 4.2 to pH, the concentrated milk that solid content is 53% is then added, after cooling the temperature to 20 DEG C, is added by mass fraction 2.5 parts of taurines and 0.8 part of vitamin C pass through spray drying (spray drying condition are as follows: inlet air temperature 170 after homogeneous DEG C, leaving air temp is 80 DEG C, and air pressure is 2.5 atmospheric pressure, and hot-air flow velocity is 0.25m3/ min) obtain lactic acid bacteria goat milk Powder, streptococcus thermophilus viable count are 1.4 × 108CFU/g, lactobacillus bulgaricus viable count are 2.6 × 107CFU/g。
In embodiment 1-6, optimization, the preparation of Longstamen Onion Bulb extracting solution: the Longstamen Onion Bulb of certain mass is taken, then presses quality and body The ratio of product proportion (grams per milliliter) 1:10 soaks in water 20-30min, is then added into and boils, and maintains boiling 30min, then With 400 mesh filter-cloth filterings, filtrate is obtained, then again by the ratio for pressing quality and volume proportion (grams per milliliter) 1:10 again in filter residue Example is added distilled water heating and boils and maintain 20min, is then filtered, merges filtrate twice, make Longstamen Onion Bulb and water ratio 1: 10 (i.e. 10ml extracting solution is made in 1g Longstamen Onion Bulb altogether), i.e. mass concentration be 10% Longstamen Onion Bulb extracting solution.
The preparation of puncture vine extracting solution: taking a certain amount of puncture vine to crush, and makes its granularity 20-60 mesh, then press quality and volume Deionized water is added in the ratio for matching (grams per milliliter) 1:10, boils with high heat on electric furnace, and then mild fire keeps boiling 30min, Then with the filter-cloth filtering of 400 mesh, deionized water is added again in quality and the ratio of volume proportion (grams per milliliter) 1:10 in the dregs of a decoction Secondary to boil and keep boiling 30min, filtering merges filtrate twice and extracting solution is distinguished constant volume, makes puncture vine and water ratio 1:10 (i.e. 10ml extracting solution is made in 1g puncture vine altogether) obtains the puncture vine extracting solution that mass concentration is 10%.
Example given above is to realize the present invention preferably example, and the present invention is not limited to the above embodiments.This field Technical staff's technical solution according to the present invention technical characteristic any nonessential addition, the replacement made, belong to this The protection scope of invention.

Claims (8)

1. a kind of lactic acid bacteria formula goat milk powder, which is characterized in that by mass fraction by 1000 parts of fresh sheep creams, 2.5-5 parts of stachyoses, 4-7.5 parts of sodium glutamates, 1-3 parts of xylo-oligosaccharides, 1-3 portions of trehaloses, 2-4 parts of taurines, 0.3-1 parts of vitamin Cs, 0.2-0.5 Part lactobacillus bulgaricus bacterium powder and 0.2-0.5 parts of streptococcus thermophilus bacterium powder compositions.
2. a kind of preparation method of lactic acid bacteria formula goat milk powder, which comprises the following steps:
Step 1: pressing mass fraction, add 2.5-5 parts of stachyoses, 4-7.5 parts of sodium glutamates, 1-3 parts into 1000 parts of fresh sheep creams Xylo-oligosaccharide and 1-3 portions of trehaloses, stir evenly to form mixture；Mixture, which is divided into two parts that proportional region is 2:1-1:1, to be mixed Close object；
Step 2: the concentrated milk that solid content is 46-53% is concentrated into after a mixture instantaneous sterilizing, it is spare；
Step 3: another mixture through sterilization be cooled to 42-46 DEG C, be respectively connected to 0.2-0.5 part lactobacillus bulgaricus powder with 0.2-0.5 parts of streptococcus thermophilus bacterium powder, 42 DEG C of constant temperature incubations reach 4.2-4.5 to pH, spare concentrated milk are added, and temperature is down to 20-30 DEG C, 2-4 parts of taurines and 0.3-1 parts of vitamin Cs are added, are uniformly mixed, lactic acid bacteria goat milk is obtained by spray drying Powder.
3. a kind of preparation method of lactic acid bacteria formula goat milk powder according to claim 2, which is characterized in that the step 3 spray drying condition are as follows: inlet air temperature is 150-170 DEG C, and leaving air temp is 60-80 DEG C, and air pressure is 1-3 atmosphere Pressure, hot-air flow velocity are 0.2-0.4m3/min。
4. a kind of preparation method of lactic acid bacteria formula goat milk powder according to claim 2, which is characterized in that the step Streptococcus thermophilus viable count is 1.2-3.6 × 10 in 3 resulting lactic acid bacteria goat milk powders8CFU/g, lactobacillus bulgaricus viable count For 2.2-7.3 × 107CFU/g。
5. a kind of preparation method of lactic acid bacteria formula goat milk powder according to claim 2, which is characterized in that the guarantor adds The preparation of Leah lactobacillus powder are as follows: add 0.2-0.4% in the Longstamen Onion Bulb extracting solution for being 10% to mass concentration by mass volume ratio Casein hydrolysate, 0.1-0.3% sodium acetate and 0.2-0.4% Triammonium citrate obtain culture medium, sterilizing cooling；By volume to The lactobacillus bulgaricus that 1-3% has been activated is accessed in culture medium, in 37 DEG C of constant temperature incubation 18-24h, refrigerated centrifuge, which is collected, to be obtained Lactobacillus bulgaricus bacterium mud, the compound freeze drying protectant of 1-3 times of weight of lactobacillus bulgaricus bacterium mud of addition, is placed in 8-15 DEG C Cold treatment 1-2h, for 24 hours using vacuum freeze drier freeze-drying, obtaining viable count is 1.2 × 1011CFU/g-5.0×1011CFU/g Lactobacillus bulgaricus bacterium powder.
6. a kind of preparation method of lactic acid bacteria formula goat milk powder according to claim 5, which is characterized in that the Longstamen Onion Bulb The preparation of extracting solution: taking the Longstamen Onion Bulb of certain mass, soaks in water 20- in the ratio of quality and volume proportion (grams per milliliter) 1:10 30min is boiled and boiling 30min is maintained to obtain filtrate with 400 mesh filter-cloth filterings；Filter residue presses quality and volume proportion again The ratio of (grams per milliliter) 1:10 is added distilled water heating and boils and maintain 20min, filters；Merge filtrate twice and constant volume, obtains matter Measure the Longstamen Onion Bulb extracting solution that concentration is 10%.
7. a kind of preparation method of lactic acid bacteria formula goat milk powder according to claim 2, which is characterized in that described is thermophilic The preparation of streptococcus bacterium powder are as follows: by mass volume ratio to mass concentration be 10% puncture vine extracting solution in add 1-2% glucose, 0.2-0.4% soy peptone, 0.6-1.0% casein hydrolysate, 0.0015-0.0040% sodium glutamate, 0.1-0.2% KH2PO4With 0.01-0.05% Tween-80, pH is adjusted to 6.5-7.0 and obtains culture medium；3-6% is accessed into culture medium by volume The streptococcus thermophilus activated, in 42 DEG C of constant temperature incubation 9-12h, refrigerated centrifuge obtains streptococcus thermophilus bacterium mud, adds thermophilic chain 1-3 times of weight of coccus bacterium mud of compound freeze drying protectant is placed in 8-15 DEG C of cold treatment 1-2h, is lyophilized using vacuum freeze drier For 24 hours, obtaining viable count is 1.0 × 1011CFU/g-3.6×1011The streptococcus thermophilus bacterium powder of CFU/g.
8. a kind of preparation method of lactic acid bacteria formula goat milk powder according to claim 7, which is characterized in that the puncture vine The preparation of extracting solution: it takes a certain amount of puncture vine to crush, makes its granularity 20-60 mesh；Puncture vine by quality and volume proportion (gram/milli Rising) deionized water is added in the ratio of 1:10, and it boils and keeps boiling 30min, with the filter-cloth filtering of 400 mesh；Filter residue by quality with The ratio of volume proportion (grams per milliliter) 1:10 is added deionized water and boils again and keep boiling 30min, filtering；Merge twice Filtrate and constant volume obtain the puncture vine extracting solution that mass concentration is 10%.
CN201811620633.7A 2018-12-28 2018-12-28 A kind of lactic acid bacteria formula goat milk powder and preparation method thereof Withdrawn CN109527098A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101810220A (en) * 2010-04-23 2010-08-25 陕西农产品加工技术研究院 Preparation method of synbiotic goat milk powder
CN102258081A (en) * 2011-08-01 2011-11-30 陕西科技大学 Synbiotics liquid goat milk beverage and preparation method thereof
CN106234589A (en) * 2016-09-23 2016-12-21 陕西科技大学 A kind of preparation method of antioxidation probiotics fermention breast
CN107568341A (en) * 2017-10-28 2018-01-12 卜球 A kind of formula milk and its production method for being advantageous to human body intestinal canal health

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101810220A (en) * 2010-04-23 2010-08-25 陕西农产品加工技术研究院 Preparation method of synbiotic goat milk powder
CN102258081A (en) * 2011-08-01 2011-11-30 陕西科技大学 Synbiotics liquid goat milk beverage and preparation method thereof
CN106234589A (en) * 2016-09-23 2016-12-21 陕西科技大学 A kind of preparation method of antioxidation probiotics fermention breast
CN107568341A (en) * 2017-10-28 2018-01-12 卜球 A kind of formula milk and its production method for being advantageous to human body intestinal canal health

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
代敏编著: "《中老年膳食营养养生全说》", 31 December 2014 *
南远顺编著: "《高血压吃什么禁什么随手查》", 31 December 2015 *
王海云: "《现代生活与健康》", 31 December 1988 *

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