CN109526992A - A kind of toad stoste and preparation method thereof with resistant effect - Google Patents
A kind of toad stoste and preparation method thereof with resistant effect Download PDFInfo
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- CN109526992A CN109526992A CN201811327774.XA CN201811327774A CN109526992A CN 109526992 A CN109526992 A CN 109526992A CN 201811327774 A CN201811327774 A CN 201811327774A CN 109526992 A CN109526992 A CN 109526992A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
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- Chemical & Material Sciences (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The toad stoste and preparation method thereof with resistant effect that the invention discloses a kind of, the raw material including following parts by weight: 5.6-7.8 parts of bacillus licheniformis, 35-44 parts of tealeaf residue, 5.9-8.8 parts of toad stoste, 3.8-5.4 parts of lauryl sodium sulfate, 2.7-3.9 parts of magnesium sulfate, 13-18.4 parts of folium artemisiae argyi, 8-10.6 parts of aryl phenol polyethenoxy and 28-33 parts of Animal Bone.Raw material sources of the present invention are extensive, by the way that tealeaves is digested to obtain tealeaves enzymolysis liquid, folium artemisiae argyi is carried out folium artemisiae argyi extract liquor is obtained by extraction, bacillus licheniformis, toad stoste, folium artemisiae argyi extract liquor etc. are mixed again, synergistic effect is played between various raw materials, the finished product of preparation has good prevention effect for crops prevention and treatment Common Diseases, and so as to improve the yield and quality of crops, prospect of the application is wide.
Description
Technical field
The present invention relates to disease-resistant crops field, specifically a kind of toad stoste with resistant effect.
Background technique
Bread is the staff of life, expresses the relationship of people and food, and reasonable diet collocation could bring health to the mankind.Food
It is self-sufficient, be only the basis of a national sustainable development.Crops are one of the main source of the basic food of the mankind,
Crops, refer to the various plants agriculturally cultivated, including Liang food Zuo Wu ﹑ industrial crops (oil crops, vegetable crop, flower, grass,
Trees) two major classes.
The plantation of crops becomes the main economic source of many people, and the disease of crops is to influence crop yield and product
An important factor for matter, corps diseases have the characteristics that type is more, it is big to influence and population outbreak often.
In order to reduce the disease of crops, people can spray various medicaments to crops, resist it is expected that improving crops
The ability of disease.But not only control efficiency is poor for existing disease-resistant medicament, but also is easy to produce drug resistance, while being directed to different diseases
Evil will also use different fungicide, expend a large amount of human and material resources and financial resources cost, this is just that the use of people is brought
It is inconvenient.
Summary of the invention
The toad stoste with resistant effect that the purpose of the present invention is to provide a kind of, to solve to mention in above-mentioned background technique
Out the problem of.
To achieve the above object, the invention provides the following technical scheme:
A kind of toad stoste with resistant effect, the raw material including following parts by weight: bacillus licheniformis 5.6-7.8
Part, 35-44 parts of tealeaf residue, 5.9-8.8 parts of toad stoste, 3.8-5.4 parts of lauryl sodium sulfate, 2.7-3.9 parts of magnesium sulfate, Chinese mugwort
13-18.4 parts of leaf, 8-10.6 parts of aryl phenol polyethenoxy and 28-33 parts of Animal Bone.
As a further solution of the present invention: the length of tealeaf residue is 1.2-5.8mm.
As a further solution of the present invention: Animal Bone includes pig bone, ox bone, sheep bone, horse bone, chicken bone, duck bone and frontal bone
In at least five kinds.
The preparation method of the toad stoste with resistant effect, the specific steps are as follows:
Animal Bone is crushed to 80-100 mesh by step 1, and the water of 3-5 times of its weight is added and boils, boils 50- after boiling
70 minutes, showy impurity is removed, then proceedes to boil 110-145 minutes, obtains bone soup；
Tealeaf residue is freeze-dried 10-15 minutes, the ring for being then 5.4-6.8 in 32-45 degrees Celsius and pH value by step 2
It is digested under border, tealeaves enzymolysis liquid is obtained by filtration in enzyme deactivation after digesting completely；
Folium artemisiae argyi is cleaned and is used supercritical CO 2 extraction technology, filters and be recovered under reduced pressure solvent by step 3,
Obtain folium artemisiae argyi extract liquor；
Lauryl sodium sulfate and magnesium sulfate are added in folium artemisiae argyi extract liquor step 4, are uniformly dispersed and electric field treatment
4-8 minutes, obtain the first mixture；
Bacillus licheniformis and toad stoste are added in tealeaves enzymolysis liquid and are stirred evenly, be then aged by step 5
18-24 hours, obtain the second mixture；
Step 6, aryl phenol polyethenoxy, bone soup and the first mixture mixed on low speed in blender is uniform, then to its
The second mixture of middle addition and high-speed stirred are uniformly to get arriving finished product.
As a further solution of the present invention: the temperature of supercritical carbon dioxide extracting is 50-64 degrees Celsius in step 3,
The pressure of extraction is 16.9-20.5MPa.
As a further solution of the present invention: electric field strength is 15-20V/m, voltage 45-66V in step 4.
As a further solution of the present invention: the speed of mixed on low speed is 66-105rpm, the speed of high-speed stirred in step 6
Degree is 1860-2040rpm.
Compared with prior art, the beneficial effects of the present invention are: raw material sources of the present invention are extensive, by the way that tealeaves is carried out enzyme
Solution obtains tea enzyme solution liquid, be obtained by extraction folium artemisiae argyi extract liquor for folium artemisiae argyi, then by bacillus licheniformis, toad stoste, folium artemisiae argyi
Extract liquor etc. mixes, and synergistic effect is played between various raw materials, and the finished product of preparation has crops prevention and treatment Common Diseases good
Prevention effect, so as to improve the yield and quality of crops, prospect of the application is wide.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
Embodiment 1
A kind of toad stoste with resistant effect, the raw material including following parts by weight: 5.6 parts of bacillus licheniformis, tea
35 parts of leaf slag, 5.9 parts of toad stoste, 3.8 parts of lauryl sodium sulfate, 2.7 parts of magnesium sulfate, 13 parts of folium artemisiae argyi, aryl phenol polyoxy second
8 parts and 28 parts of Animal Bone of alkene.Animal Bone include pig bone, sheep bone, horse bone, chicken bone and duck bone mixture.
The preparation method of the toad stoste with resistant effect, the specific steps are as follows:
Animal Bone is crushed to 90 mesh by step 1, and the water of 4 times of its weight is added and boils, boils 58 minutes, goes after boiling
Except showy impurity, then proceedes to boil 135 minutes, obtain bone soup；
Tealeaf residue is freeze-dried 12 minutes, then digests in the environment of 38 degrees Celsius and pH value are 5.7 by step 2,
The completely rear enzyme deactivation of enzymatic hydrolysis, is obtained by filtration tealeaves enzymolysis liquid；
Supercritical CO 2 extraction technology is cleaned and used to folium artemisiae argyi by step 3, and the temperature of extraction is 58 degrees Celsius,
The pressure of extraction is 18.4MPa, filters and is recovered under reduced pressure solvent, obtain folium artemisiae argyi extract liquor；
Lauryl sodium sulfate and magnesium sulfate are added in folium artemisiae argyi extract liquor step 4, are uniformly dispersed and electric field treatment 6
Minute, electric field strength 18V/m, voltage 58V obtain the first mixture；
Bacillus licheniformis and toad stoste are added in tealeaves enzymolysis liquid and are stirred evenly, be then aged by step 5
21 hours, obtain the second mixture；
Step 6, aryl phenol polyethenoxy, bone soup and the first mixture mixed on low speed in blender is uniform, then to its
The second mixture of middle addition and high-speed stirred are uniformly to get arriving finished product.
Embodiment 2
A kind of toad stoste with resistant effect, the raw material including following parts by weight: 6.2 parts of bacillus licheniformis, tea
38.4 parts of leaf slag, 6.6 parts of toad stoste, 4.3 parts of lauryl sodium sulfate, 3.1 parts of magnesium sulfate, 14.6 parts of folium artemisiae argyi, aryl phenol are poly-
8.7 parts and 30 parts of Animal Bone of ethylene oxide.The length of tealeaf residue is 3.4mm.Animal Bone includes ox bone, sheep bone, horse bone, chicken bone, duck
The mixture of bone and frontal bone.
The preparation method of the toad stoste with resistant effect, the specific steps are as follows:
Animal Bone is crushed to 100 mesh by step 1, and the water of 3 times of its weight is added and boils, boils after boiling 65 minutes,
The showy impurity of removal, then proceedes to boil 136 minutes, obtains bone soup；
Tealeaf residue is freeze-dried 12 minutes, then digests in the environment of 41 degrees Celsius and pH value are 6.4 by step 2,
The completely rear enzyme deactivation of enzymatic hydrolysis, is obtained by filtration tealeaves enzymolysis liquid；
Folium artemisiae argyi is cleaned and is used supercritical CO 2 extraction technology, filters and be recovered under reduced pressure solvent by step 3,
Obtain folium artemisiae argyi extract liquor；
Lauryl sodium sulfate and magnesium sulfate are added in folium artemisiae argyi extract liquor step 4, are uniformly dispersed and electric field treatment 8
Minute, electric field strength 15V/m, voltage 45V obtain the first mixture；
Bacillus licheniformis and toad stoste are added in tealeaves enzymolysis liquid and are stirred evenly, be then aged by step 5
21 hours, obtain the second mixture；
Step 6 mixes aryl phenol polyethenoxy, bone soup and the first mixture in blender with the revolving speed of 75rpm
It is even, then the second mixture is added thereto and is stirred evenly with the revolving speed of 1950rpm to get finished product is arrived.
Embodiment 3
A kind of toad stoste with resistant effect, the raw material including following parts by weight: 7.5 parts of bacillus licheniformis, tea
42.1 parts of leaf slag, 7.7 parts of toad stoste, 5.2 parts of lauryl sodium sulfate, 3.5 parts of magnesium sulfate, 17.6 parts of folium artemisiae argyi, aryl phenol are poly-
9.8 parts and 31.6 parts of Animal Bone of ethylene oxide.The length of tealeaf residue is 3.45mm.Animal Bone include pig bone, ox bone, sheep bone, horse bone,
The mixture of chicken bone, duck bone and frontal bone.
The preparation method of the toad stoste with resistant effect, the specific steps are as follows:
Animal Bone is crushed to 100 mesh by step 1, and the water of 5 times of its weight is added and boils, boils after boiling 63 minutes,
The showy impurity of removal, then proceedes to boil 128 minutes, obtains bone soup；
Tealeaf residue is freeze-dried 11 minutes, then digests in the environment of 44 degrees Celsius and pH value are 6.3 by step 2,
The completely rear enzyme deactivation of enzymatic hydrolysis, is obtained by filtration tealeaves enzymolysis liquid；
Supercritical CO 2 extraction technology is cleaned and used to folium artemisiae argyi by step 3, and the temperature of extraction is 58 degrees Celsius,
The pressure of extraction is 18.7MPa, filters and is recovered under reduced pressure solvent, obtain folium artemisiae argyi extract liquor；
Lauryl sodium sulfate and magnesium sulfate are added in folium artemisiae argyi extract liquor step 4, are uniformly dispersed and electric field treatment
Obtain the first mixture within 4-8 minutes, electric field strength 17V/m, voltage 55V；
Bacillus licheniformis and toad stoste are added in tealeaves enzymolysis liquid and are stirred evenly, be then aged by step 5
22 hours, obtain the second mixture；
Step 6 mixes aryl phenol polyethenoxy, bone soup and the first mixture in blender with the revolving speed of 84rpm
It is even, then the second mixture is added thereto and is stirred evenly with the revolving speed of 2010rpm to get finished product is arrived.
Embodiment 4
A kind of toad stoste with resistant effect, the raw material including following parts by weight: 7.8 parts of bacillus licheniformis, tea
44 parts of leaf slag, 8.8 parts of toad stoste, 5.4 parts of lauryl sodium sulfate, 3.9 parts of magnesium sulfate, 18.4 parts of folium artemisiae argyi, aryl phenol polyoxy
10.6 parts and 33 parts of Animal Bone of ethylene.The length of tealeaf residue is 5.2mm.
The preparation method of the toad stoste with resistant effect, the specific steps are as follows:
Animal Bone is crushed to 90 mesh by step 1, and the water of 3 times of its weight is added and boils, boils 64 minutes, goes after boiling
Except showy impurity, then proceedes to boil 133 minutes, obtain bone soup；
Tealeaf residue is freeze-dried 15 minutes, then digests in the environment of 40 degrees Celsius and pH value are 6.6 by step 2,
The completely rear enzyme deactivation of enzymatic hydrolysis, is obtained by filtration tealeaves enzymolysis liquid；
Supercritical CO 2 extraction technology is cleaned and used to folium artemisiae argyi by step 3, supercritical carbon dioxide extracting
Temperature is 57 degrees Celsius, and the pressure of extraction is 18.6MPa, filters and is recovered under reduced pressure solvent, obtain folium artemisiae argyi extract liquor；
Lauryl sodium sulfate and magnesium sulfate are added in folium artemisiae argyi extract liquor step 4, are uniformly dispersed and electric field treatment 6
Minute, electric field strength 18V/m, voltage 62V obtain the first mixture；
Bacillus licheniformis and toad stoste are added in tealeaves enzymolysis liquid and are stirred evenly, be then aged by step 5
18 hours, obtain the second mixture；
Step 6, aryl phenol polyethenoxy, bone soup and the first mixture mixed on low speed in blender is uniform, then to its
The second mixture of middle addition and high-speed stirred are uniformly to get arriving finished product.
Comparative example
As a comparison case by existing medicament.
The identical soil of selection soil regime is divided into 1-5 group, plants agriculture using identical seed and identical management method
Crop observes and records the sick situation of crops, the results are shown in Table 1.
Table 1
From table 1 it follows that the sick rate of 1-4 group is far below 5 groups of sick rate, show product of the invention for anti-
The Common Diseases for controlling crops have good prevention effect.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (7)
1. a kind of toad stoste with resistant effect, which is characterized in that the raw material including following parts by weight: bacillus licheniformis
5.6-7.8 parts, 35-44 parts of tealeaf residue, 5.9-8.8 parts of toad stoste, 3.8-5.4 parts of lauryl sodium sulfate, magnesium sulfate 2.7-
3.9 parts, 13-18.4 parts of folium artemisiae argyi, 8-10.6 parts of aryl phenol polyethenoxy and 28-33 parts of Animal Bone.
2. the toad stoste according to claim 1 with resistant effect, which is characterized in that the length of the tealeaf residue is
1.2-5.8mm。
3. the toad stoste according to claim 1 or 2 with resistant effect, which is characterized in that the Animal Bone includes
At least five kinds in pig bone, ox bone, sheep bone, horse bone, chicken bone, duck bone and frontal bone.
4. a kind of preparation method of the toad stoste a method according to any one of claims 1-3 with resistant effect, which is characterized in that
Specific step is as follows:
Animal Bone is crushed to 80-100 mesh by step 1, and the water of 3-5 times of its weight is added and boils, and 50-70 points are boiled after boiling
Clock removes showy impurity, then proceedes to boil 110-145 minutes, obtains bone soup；
Tealeaf residue is freeze-dried 10-15 minutes, then in the environment of 32-45 degrees Celsius and pH value are 5.4-6.8 by step 2
Enzymatic hydrolysis, enzyme deactivation after digesting completely, is obtained by filtration tealeaves enzymolysis liquid；
Folium artemisiae argyi is cleaned and is used supercritical CO 2 extraction technology, filters and be recovered under reduced pressure solvent, obtain by step 3
Folium artemisiae argyi extract liquor；
Lauryl sodium sulfate and magnesium sulfate are added in folium artemisiae argyi extract liquor step 4, are uniformly dispersed and electric field treatment 4-8 divides
Clock obtains the first mixture；
Bacillus licheniformis and toad stoste are added in tealeaves enzymolysis liquid and are stirred evenly, be then aged 18-24 by step 5
Hour, obtain the second mixture；
Step 6, aryl phenol polyethenoxy, bone soup and the first mixture mixed on low speed in blender is uniform, then add thereto
Enter the second mixture and high-speed stirred is uniformly to get to finished product.
5. the preparation method of the toad stoste according to claim 4 with resistant effect, which is characterized in that the step
The temperature of supercritical carbon dioxide extracting is 50-64 degrees Celsius in three, and the pressure of extraction is 16.9-20.5MPa.
6. the preparation method of the toad stoste according to claim 4 or 5 with resistant effect, which is characterized in that described
Electric field strength is 15-20V/m, voltage 45-66V in step 4.
7. the preparation method of the toad stoste according to claim 4 with resistant effect, which is characterized in that the step
The speed of mixed on low speed is 66-105rpm in six, and the speed of high-speed stirred is 1860-2040rpm.
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| CN105010436A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Grape downy mildew prevention and control bacteriostat, and preparation method and application thereof |
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| 陈道章: "《中国古代化学史》", 31 July 2000, 福建科学技术出版社 * |
| 马安民 等: "《园林植物杀菌剂应用技术》", 31 October 2016, 河南科学技术出版社 * |
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Application publication date: 20190329 |