CN109521016A - A kind of thrips observation method - Google Patents

A kind of thrips observation method Download PDF

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Publication number
CN109521016A
CN109521016A CN201811377924.8A CN201811377924A CN109521016A CN 109521016 A CN109521016 A CN 109521016A CN 201811377924 A CN201811377924 A CN 201811377924A CN 109521016 A CN109521016 A CN 109521016A
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CN
China
Prior art keywords
thrips
flower
tube body
observation
lower tube
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Pending
Application number
CN201811377924.8A
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Chinese (zh)
Inventor
余清
莫笑晗
夏振远
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Yunnan Academy of Tobacco Agricultural Sciences
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Yunnan Academy of Tobacco Agricultural Sciences
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Priority to CN201811377924.8A priority Critical patent/CN109521016A/en
Publication of CN109521016A publication Critical patent/CN109521016A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098Plants or trees
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • G01N2021/8466Investigation of vegetal material, e.g. leaves, plants, fruits

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

It is first to randomly select tobacco field periphery flower the invention discloses a kind of thrips observation method, the flower is random one in solitary flower, entire inflorescence or inflorescence;Then observation device will be picked up close to flower;Flower is held quickly to observation device shake is picked up, and the thrips in flower is shaken off on picking up observation device, the thrips picked up on observation device can be observed, determine thrips quantity;The quantity for choosing tobacco field periphery flower is 20 ~ 40.Method of the invention is the habit for liking being gathered in plant flower using thrips, by being observed on the flower to field periphery plant whether there is or not thrips harm and thrips quantity, is conducive to endanger thrips field progress prediction, Instructing manufacture accurate prevention and control;It is larger especially for tobacco plant, while thrips has the advantages that easy to operate, accuracy is high the case where dispersion endangers in cigarette strain.

Description

A kind of thrips observation method
Technical field
The invention belongs to agriculture and forestry injurious insect Prevention Technique fields, and in particular to a kind of thrips observation method.
Background technique
The thrips is the general designation of Insecta Thysanoptera, and about 6000 kinds known to the whole world, China is about 600 kinds known, mainly There are Thripidae and Phlaeothripidae.Larva is white, yellow or orange, adult yellow, brown or black;Like in plant feeding Water, flower, fruit and the leaf of plant of causing harm, more happiness endanger plant flower.Body is small, the long 0.5-2mm of body;Head is slightly in deutostoma Formula, mouthpart file suction, can frustrate brokenly plant epidermis, inhale and permit juice.In Yunnan, occurred all year round for thrips, eggplant, cucumber, The crops such as kidney bean, capsicum, watermelon, tobacco, onion, the main species of occurring and damage have melon thrips (also known as brown in melon fruits and vegetables Palmitic acid thrips, pale brown thrips), onion thrips (also known as onion thrips, cotton thrips), Frankliniella occidentalis, golden thistle horse etc..Thrips likes warm, dry The weather of drought, thermophilic are 23 DEG C ~ 28 DEG C, and suitable air humidity is 40% ~ 70%.Thrips permits crop juice to directly affect work in addition to inhaling Outside object growth and development, inhales during plant permits juice and go back transmitted virus, such as tomato spotted wilf virus (TSWV), to crop yield High risks are caused, frequently result in total crop failure as tobacco is transmitted after tomato spotted wilt.
In agricultural production in order to prevent and treat thrips harm and prevention and treatment thrips transmitted virus, need to manually arriving field observation, whether there is or not Jis Horse harm, but because the thrips bodily form is small, it is difficult to thrips harm is observed directly on crop.Such as during leaf tobacco production, it is It accurately effectively prevents thrips harm and its propagates tomato spotted wilf virus it is necessary to carry out the forecast of field thrips Harm, due to Tobacco plant is larger, and thrips disperses harm in cigarette strain, is difficult with the naked eye to observe directly thrips.It can for this purpose, researching and developing one kind The thrips observation method to solve the above problems is very important.
Summary of the invention
The purpose of the present invention is to provide a kind of thrips observation methods.
The object of the present invention is achieved like this, first randomly selects tobacco field periphery flower, and the flower is solitary flower, whole Random one in a inflorescence or inflorescence;Then observation device will be picked up close to flower;Hold flower quickly to pick up observation dress Shake is set, the thrips in flower is shaken off on picking up observation device, the thrips picked up on observation device can be observed, Determine thrips quantity;The quantity for choosing tobacco field periphery flower is 20 ~ 40.
Compared with prior art, the present invention has following technical effect that
1, method of the invention is the habit for liking being gathered in plant flower using thrips, passes through the flower to field periphery plant Piece upper observation is conducive to endanger thrips field and carries out prediction whether there is or not thrips harm and thrips quantity, and Instructing manufacture is precisely prevented It controls;It is larger especially for tobacco plant, while thrips has easy to operate, accuracy the case where dispersion endangers in cigarette strain High advantage;
2, the observation device that picks up of the invention passes through loading flower, can easily the thrips in flower be shaken off and be collected;Observation In the process, glass observation piece plays reflective effect, while the enhancing of black substrate is conducive to observation, solves to the contrast of thrips It is not easy the drawbacks of directly observing in field, field naked eyes since the thrips bodily form is small, observation efficiency is effectively improved, reduces work Amount reduces observation, compiling costs;When insufficient light, light filling can be carried out, enhances observing effect;Pick up the upper tube of observation device Body, lower tube body are all made of screw thread detachable structure between observation base, are not only convenient for taking out thrips, and be conducive to cleaning, maintenance; Drawing and pulling type isolation board plays isolation effect, prevents thrips from escaping;Upper tube body is suitable for that inflorescence is longer and the larger situation of flower, just It is packed into flower.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of apparatus of the present invention;
In figure: 1- upper tube body, 2- lower tube body, 3- observation base, 4- glass observe piece, 5- substrate, 6- light filling bottom lamp, 7- light filling side Lamp, 8- charge port, 9- switch, 10- isolation board, 11- positive stop lug boss, 12- strainer.
Specific embodiment
The present invention will be further described below with reference to the drawings, but the present invention is limited in any way, base In present invention teach that it is made it is any transform or replace, all belong to the scope of protection of the present invention.
The present invention as shown in Fig. 1 is first to randomly select tobacco field periphery flower, and the flower is solitary flower, entire inflorescence Or random one in inflorescence;Then observation device will be picked up close to flower;Hold flower quickly to pick up observation device shake, Thrips in flower is shaken off on picking up observation device, the thrips picked up on observation device can be observed, determine Ji Horse quantity;The quantity for choosing tobacco field periphery flower is 20 ~ 40.
Preferably, the holding flower is holder or the anthocaulus for holding flower.
Preferably, the described observation device that picks up includes lower tube body 2, observation base 3, glass observation piece 4, substrate 5, described Observation base 3 is equipped with groove, and the lower end of lower tube body 2 and the top of groove inner wall are detachably connected by helicitic texture, described Lower tube body 2 is transparent material, and the horizontal lower part being set in groove of glass observation piece 4, the substrate 5 fits in glass The bottom of piece 4 is observed, the material of the substrate 5 is dark light transmission paper.
It preferably, further include upper tube body 1, the upper tube body 1 presents slightly lower fine texture, the upper end of the lower tube body 2 It is detachably connected with the lower end of upper tube body 1 by helicitic texture, the upper tube body 1 is transparent material.
It preferably, further include light filling bottom lamp 6, light filling side lamp 7, the light filling bottom lamp 6 is set to the bottom in groove, and mends Light bottom lamp 6 is located at the lower section of dark light transmission paper, and light filling bottom lamp 6 is vertical upward, and the insertion of light filling side lamp 7 is arranged in the groove Wall, light filling side lamp 7 observe piece 4 towards glass.
It preferably, further include rechargeable battery, controller, charge port 8, switch 9, the rechargeable battery, controller are all provided with In in observation base 3, be equipped with charge port 8, switch 9 on the outside of observation base 3, controller respectively with light filling bottom lamp 6, light filling side lamp 7, charge Battery, charge port 8, switch 9 are electrically connected.
Preferably, the side of the lower tube body 2 is equipped with slot, and the isolation board 10 is horizontally inserted by slot to down tube In body 2, make to be isolated into two regions in lower tube body 2.
Preferably, the color of the substrate 5 is black.
Preferably, it is bonded between glass observation piece 4 and substrate 5 by viscose.
Preferentially, 2 inner wall of lower tube body is equipped with positive stop lug boss 11, and strainer 12, and strainer 12 are equipped in lower tube body 2 In on positive stop lug boss 11, strainer 12 plays the role of that flower fragment and other impurities are isolated, while not influencing thrips whereabouts.
Preferably, the controller is single-chip microcontroller.
The present invention picks up the working principle and the course of work of observation device: holding flower on the other hand first, another holding picks up Device is observed, then flower is slowly packed into downwards to upper tube body 1, lower tube body 2 until 4 top of glass observation piece;Then it quickly trembles Dynamic flower, thrips fall on glass observation piece 4;Flower is taken out, while isolation board 10 is inserted into slot, makes 2 envelope of lower tube body It closes;Then lower tube body is penetrated, the thrips on glass observation piece 4 is observed and counted;During observation, glass is observed piece and is risen To reflective effect, while the enhancing of black substrate is conducive to observation to the contrast of thrips;When insufficient light, 9 are turned on the switch, is mended A part of light of light bottom lamp 6 penetrates substrate 5, and light filling side lamp 7 carries out light filling to glass observation piece 4, enhances observing effect;So Back spin backs out tube body 2, separates lower tube body 2 with observation base 3, and thrips is poured out and cleared up, and then reinstalls lower tube body 2, and extract out Isolation board 10 can be handled next flower;After the present apparatus can also collect thrips, takes back laboratory and further look at Classification processing.
Below with reference to 1 ~ embodiment of embodiment 4, the invention will be further described.
Embodiment 1
Tobacco field periphery flower is first randomly selected, the flower is random one in solitary flower, entire inflorescence or inflorescence;Then Observation device will be picked up close to flower;Flower is held quickly to observation device shake is picked up, and the thrips in flower is shaken off and is being connect It takes on observation device, the thrips picked up on observation device can be observed, determine thrips quantity;The selection tobacco field periphery The quantity of flower is 20.
Embodiment 2
Tobacco field periphery flower is first randomly selected, the flower is random one in solitary flower, entire inflorescence or inflorescence;Then Observation device will be picked up close to flower;Flower is held quickly to observation device shake is picked up, and the thrips in flower is shaken off and is being connect It takes on observation device, the thrips picked up on observation device can be observed, determine thrips quantity;The selection tobacco field periphery The quantity of flower is 40.
Embodiment 3
Tobacco field periphery flower is first randomly selected, the flower is random one in solitary flower, entire inflorescence or inflorescence;Then Observation device will be picked up close to flower;Flower is held quickly to observation device shake is picked up, and the thrips in flower is shaken off and is being connect It takes on observation device, the thrips picked up on observation device can be observed, determine thrips quantity;The selection tobacco field periphery The quantity of flower is 30.
Embodiment 4: tobacco field comparative test
Group A is to observe thrips using conventional method, i.e., is directly observed in tobacco field cigarette strain;Group B is using the present invention Method observe thrips, observation the results are shown in Table 1.
1 thrips of table observes result
As shown in Table 1, the thrips quantity that group B is observed is 43 times of conventional method observation thrips quantity, substantially increases Ji The accuracy of horse observation investigation, can effectively reduce the amount of labour used, reduce observation, compiling costs, solve due to the thrips bodily form it is small Field, field are not easy the drawbacks of directly observing, and can be used for crop prevention and treatment thrips harm and carry out effective prediction.

Claims (10)

1. a kind of thrips observation method, it is characterised in that the following steps are included: first randomly selecting tobacco field periphery flower, the flower Piece for random one in solitary flower, entire inflorescence or inflorescence;Then observation device will be picked up close to flower;It is quick to hold flower To observation device shake is picked up, the thrips in flower is shaken off on picking up observation device, can be observed on device picking up Thrips is observed, and determines thrips quantity;The quantity for choosing tobacco field periphery flower is 20 ~ 40.
2. thrips observation method according to claim 1, it is characterised in that the holding flower is the flower for holding flower Support or anthocaulus.
3. thrips observation method according to claim 1, it is characterised in that the observation device that picks up includes lower tube body (2), observation base (3), glass observe piece (4), substrate (5), and the observation base (3) is equipped with groove, the lower end of lower tube body (2) It is detachably connected with the top of groove inner wall by helicitic texture, the lower tube body (2) is transparent material, and the glass is seen The horizontal lower part being set in groove of piece (4) is examined, the substrate (5) fits in the bottom of glass observation piece (4), the substrate (5) material is dark light transmission paper.
4. thrips observation method according to claim 3, it is characterised in that further include upper tube body (1), the upper tube body (1) slightly lower fine texture, the upper end of the lower tube body (2) is presented detachably to connect with the lower end of upper tube body (1) by helicitic texture It connects, the upper tube body (1) is transparent material.
5. thrips observation method according to claim 3, it is characterised in that further include light filling bottom lamp (6), light filling side lamp (7), the light filling bottom lamp (6) is set to the bottom in groove, and light filling bottom lamp (6) is located at the lower section of dark light transmission paper, light filling Bottom lamp (6) is vertical upward, and light filling side lamp (7) insertion is arranged in the groove wall, and light filling side lamp (7) observes piece towards glass (4).
6. thrips observation method according to claim 5, it is characterised in that further include rechargeable battery, controller, charge port (8), (9) are switched, the rechargeable battery, controller are set in observation base (3), and charge port is equipped on the outside of observation base (3) (8), (9) are switched, controller is electric with light filling bottom lamp (6), light filling side lamp (7), rechargeable battery, charge port (8), switch (9) respectively Connection.
7. thrips observation method according to claim 3, it is characterised in that the side of the lower tube body (2), which is equipped with, inserts Slot, the isolation board (10) are horizontally inserted by slot to lower tube body (2), make to be isolated into two regions in lower tube body (2).
8. thrips observation method according to claim 3, it is characterised in that the color of the substrate (5) is black.
9. thrips observation method according to claim 3, it is characterised in that lower tube body (2) inner wall is equipped with limit convex Platform (11), lower tube body (2) is interior to be equipped with strainer (12), and strainer (12) is located on positive stop lug boss (11).
10. thrips observation method according to claim 3, it is characterised in that lower tube body (2) inner wall is equipped with limit Boss (11), lower tube body (2) is interior to be equipped with strainer (12), and strainer (12) is located on positive stop lug boss (11).
CN201811377924.8A 2018-11-19 2018-11-19 A kind of thrips observation method Pending CN109521016A (en)

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Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
CN201811377924.8A CN109521016A (en) 2018-11-19 2018-11-19 A kind of thrips observation method

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202363026U (en) * 2011-10-27 2012-08-01 云南省烟草农业科学研究院 Herbarium display device
CN103210882A (en) * 2013-04-24 2013-07-24 福建省农业科学院植物保护研究所 Insect raising and observing device and mango white aulacaspis raising and observing method
CN203233912U (en) * 2012-12-26 2013-10-16 北京市农林科学院 Device for collection and subpackage of thrips testing insects

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202363026U (en) * 2011-10-27 2012-08-01 云南省烟草农业科学研究院 Herbarium display device
CN203233912U (en) * 2012-12-26 2013-10-16 北京市农林科学院 Device for collection and subpackage of thrips testing insects
CN103210882A (en) * 2013-04-24 2013-07-24 福建省农业科学院植物保护研究所 Insect raising and observing device and mango white aulacaspis raising and observing method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李江涛 等: "西花蓟马在康乃馨不同品种上的田间分布", 《昆虫知识》 *

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Application publication date: 20190326