CN109512696A - A kind of skin care item and preparation method thereof containing complex polysaccharide series - Google Patents
A kind of skin care item and preparation method thereof containing complex polysaccharide series Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
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- A61Q19/00—Preparations for care of the skin
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The present invention provides a kind of skin care item and preparation method thereof containing complex polysaccharide series, belongs to technical field of skin care；The skin care item containing complex polysaccharide series include following weight percent raw material: sea cucumber polysaccharide, tremella polysaccharides, ferulic acid, panthenol, glycerol, hyaluronic acid, solubilizer, emulsion stabilizer, preservative and balance deionized water.Complex polysaccharide is made in sea cucumber polysaccharide and tremella polysaccharides by the present invention, under the action of ferulic acid, panthenol, so that each component moisturizing and antioxidant effect are more preferably.Polysaccharide is extracted using ultrasonic wave assisted recombination enzymatic hydrolysis, alcohol precipitation, obtained purity of polysaccharide is high, property is stablized.Skin care item containing complex polysaccharide series can increase skin surface hyaluronic acid contents, play the role of moisturizing, keep moisture, improve significantly for skin that is dry and that wrinkle occurred；Diastole capillary of skin promotes blood circulation, and has the effects that anti-oxidant, removing free radical, reaches apparent moisturizing cosmetic result.
Description
Technical field
The present invention relates to technical field of skin care, in particular to a kind of skin care item containing complex polysaccharide series and its preparation
Method.
Background technique
Sea cucumber belongs to Holothuroidea (Holothuroidea), is the ocean echinoderm to 8000 meters by the sea that lives, away from the present
Existing more than 600,000,000 years history, sea cucumber are food with seabed algae and planktonic organism.Sea cucumber, which has, improves memory, sexual gland is delayed to decline
Always, prevent artery sclerosis and it is antitumor the effects of.Sea cucumber polysaccharide is a kind of important component of wall of sea cucumber Stichopus japonicus, and content can Zhan Gan
Join 6% or more of total organic matter, including sea cucumber chondroitin sulfate (sea cucumber chondroitin sulfate, SC-
CHS) and two kinds of components of sea cucumber fucoidan (sea cucumber fucoidan, SC-FUC), it is living that there are many biologies
Property and pharmacological action.
Tremella is Mycophyta, alias tremella, tremella, tremella etc..Tremella polysaccharides are the principle active components of tremella, are had
Point of exocellular polysaccharide and cell wall polysaccharide, has extensive physiological activity, can improve immunity of organisms, antitumor, keeps skin water
Point, taking tremella for a long time can also have with moisturizing, and the effect of dispel chloasma faciei, freckle and remove free radical, delay
Aging adjusts the functions such as blood pressure and blood lipoid level.
In recent years, with advancing age and the increasing of environmental pollution and life stress, people are in order to adapt to work, often
It is come out early and return late, and sitting office lacks movement, and sub-health status gets worse, and skin aging is more and more obvious, become it is dry,
It lacks flexibility, generate wrinkle and color is obscure, and anti-aging beauty-care skin care is also increasingly valued by people.And the people
Living standard is higher and higher, applies relatively extensively on skin care industry about polysaccharide in the prior art, but the polysaccharide of single variety, and effect is single
One, effect is slow, is not able to satisfy demand of the modern people to beauty and skin care.Therefore, invention is a kind of shield containing complex polysaccharide
Skin frost, the molecular weight of polysaccharide have from tens of thousands of to 1,000,000 or more and keep moisture, Transdermal absorption, remove a variety of function such as free radical
Can, thus the effective solution above problem.
Summary of the invention
For mirror with this, the present invention proposes a kind of skin care item and preparation method thereof containing complex polysaccharide series, and the present invention utilizes
Transdermal absorption, the oxidation resistance of sea cucumber polysaccharide, stimulate the regeneration of skin collagen, in conjunction with the moisturizing stability of tremella polysaccharides, increase
Add skin elasticity, enhances skin immunity, slow down the skin care item of skin aging.
The technical scheme of the present invention is realized as follows: a kind of skin care item containing complex polysaccharide series, including it is following heavy
Measure percentage raw material: sea cucumber polysaccharide 17%~28%, tremella polysaccharides 11%~23%, ferulic acid 2.5%~5.0%, panthenol 0.8
~1.2%, glycerol 0.7~1.0%, hyaluronic acid 1.1%~2.5%, solubilizer 6%~19%, emulsion stabilizer 7%~
25%, preservative 0.3%~0.7% and balance deionized water.
Further, a kind of skin care item containing complex polysaccharide series, including following weight percent raw material: sea cucumber polysaccharide
19%~25%, tremella polysaccharides 13%~19%, ferulic acid 3.1%~4.7%, panthenol 1.0~1.2%, glycerol 0.8~
1.0%, hyaluronic acid 1.5%~2%, solubilizer 7%~17%, emulsion stabilizer 10%~21%, preservative 0.3%~
0.6% and balance deionized water.
Further, a kind of skin care item containing complex polysaccharide series, including following weight percent raw material: sea cucumber polysaccharide
21%, tremella polysaccharides 15%, ferulic acid 3.8%, panthenol 1.1%, glycerol 0.9%, hyaluronic acid 1.9%, solubilizer 9%, cream
Change stabilizer 14%, preservative 0.3% and balance deionized water.
The present invention also provides a kind of skin care item containing complex polysaccharide series, which is characterized in that the system of the sea cucumber polysaccharide
Preparation Method the following steps are included:
S1: crossing 40~80 meshes for sea cucumber dry powder, be placed in water, and solid-liquid ratio 1:40~70 obtain sea cucumber dry powder solution；
S2: ultrasonic wave complex enzyme hydrolysis: being added in sea cucumber dry powder solution plus acetic acid-sodium acetate buffer solution adjust pH to
4.8~5.2, complex enzyme A is added, the complex enzyme A is 12~15:22~28:9~13 papain, pancreas by mass ratio
Protease, lipase are made, and are digested under the action of ultrasonic wave auxiliary, obtain sea cucumber Thick many candies extracting solution；
S3: ultrasonic wave enzyme deactivation: by sea cucumber Thick many candies extracting solution in 15~28k Hz, 0.42~1.05W/m L, 30 DEG C~45
Enzyme deactivation is carried out under the conditions of DEG C；
S4: extraction: by the sea cucumber Thick many candies extracting solution after enzyme deactivation in 50 DEG C~80 DEG C 60~80min of extraction, extraction 2 is repeated
~4 times, filtering, merging filtrate obtains filtrate, takes supernatant to be filtered after centrifugation；
S5: alcohol precipitation: being added 4~7 times of volume 50%~95%v/v ethanol solutions for filtered sea cucumber polysaccharide leaching liquor,
8~12h is stood, secondary centrifuging, precipitating is redissolved with distilled water, and is dialysed and removed small molecular weight impurity, is filtered, and 400~600 mesh are crossed
Sieve obtains sea cucumber polysaccharide after trapped fluid freeze-drying.
5, a kind of skin care item containing complex polysaccharide series according to claim 1, which is characterized in that the tremella
The preparation method of polysaccharide the following steps are included:
S1: taking tremella appropriate, and cleaning dry, pulverize, and take tremella powder, crosses 60~80 meshes；
S2: Subcritical water chromotagraphy: Xiang Yiner powder is added water, dodecanoic acid and Pfansteihl, ultrasound-enhanced subcritical water and mentions
It takes, solid-liquid ratio 1:50~80 obtain tremella polysaccharides extracting solution；
S3: removing protein: being added complex enzyme B in tremella polysaccharides extracting solution, the complex enzyme B is papain, tryptose
Enzyme, lipase, ratio is 1.1~3.5:4.1~5.3:1.7~2.5, under conditions of temperature is 40~60 DEG C, pH is 7~9
2~3h is digested, is repeated 2~4 times, stratification divides sub-cloud denatured protein and intersection organic solvent, obtains de- albumen silver
Fungus polysaccharides liquid；
S4: alcohol precipitation: being added ethyl acetate and water in de- albumen tremella polysaccharides liquid, extracts, collection water phase, filtering and concentrating,
Concentrate is spray-dried to get tremella polysaccharides.
Further, the additive amount of the complex enzyme A is the 0.6~1% of sea cucumber dry powder quality.
Further, the additive amount of the complex enzyme B is the 0.5~1.2% of tremella dry powder quality.
Further, in the ultrasonic wave complex enzyme hydrolysis, hydrolysis temperature be 50 DEG C~60 DEG C, ultrasonic power be 400~
600W, 1.5~2h of time, frequency are 25~28kHz.
The present invention also provides a kind of preparation methods of skin care item containing complex polysaccharide series, which is characterized in that including with
Lower step:
S1: at room temperature, deionized water is added in 10%~30% sea cucumber polysaccharide and tremella polysaccharides, is heated to 40
It is dissolved at~60 DEG C, obtains solution A；
S2: panthenol, glycerol, hyaluronic acid, emulsion stabilizer are dissolved in 70~80 DEG C of agitating and heatings, are added after being cooled to
Preservative obtains solution B.
S3: solution A, B are mixed, and ferulic acid, solubilizer, pH adjusting agent, stirring, homogeneous is added.
Further, the solubilizer is ethyoxyl glycol, dipropylene glycol.
Further, the emulsion stabilizer is acrylic acid (rouge) class, Dimethicone Crosspolymer.
Further, the pH adjusting agent is succinic acid, citric acid.
Further, the preservative is Dimethicone Crosspolymer, ethyl hydroxy benzoate
It compared with prior art, will be extra large the beneficial effects of the present invention are: the present invention contains the series skin nursing products of complex polysaccharide
Complex polysaccharide is made in gracilis polysaccharide and tremella polysaccharides, and arrange in pairs or groups ferulic acid, panthenol are aided with glycerol, hyaluronic acid, solubilizer, emulsification surely
Determine agent, preservative, scientific matching, skin care item obtained have high antioxidant, can absorbing transmission from skin, increase in skin thoroughly
The content of bright matter acid plays the role of moist, moisturizing, and then improves dry and the skin of wrinkle occurred, keeps its recovery original
It is smooth and elastic；Diastole capillary of skin promotes blood circulation, to reach significant work anti-oxidant, to free radical resisting
With.Wherein, complex polysaccharide of the present invention has inoxidizability, the humectants of tremella polysaccharides under the infiltration skin effect of sea cucumber polysaccharide,
Keep the permeability of skin higher, moistening effect is more preferable, and sea cucumber polysaccharide and tremella polysaccharides combine, and collaboration improves product inoxidizability；
Simultaneously under the action of ferulic acid, free radical is eliminated, unites protection complex polysaccharide and refuses collagen degradation, and panthenol assists enhancing
The moisture-keeping efficacy of complex polysaccharide soothes the skin dry condition and enhancing skin hydration ability, at the same also act as it is certain it is anti-inflammatory,
Repair.In addition, the method that the present invention extracts polysaccharide using ultrasonic wave assisted recombination enzymatic hydrolysis, removing protein, alcohol precipitation, further mentions
The purity and recovery rate of high polysaccharide；Wherein, complex enzyme, so that enzymatic hydrolysis is more thorough, further mentions under the auxiliary of ultrasonic wave
The high efficiency of enzymatic hydrolysis, obtains high-purity and takes off proteoglycan.
Specific embodiment
In order to be best understood from the technology of the present invention content, specific embodiment is provided below, the present invention is described further.
Experimental method used in the embodiment of the present invention is conventional method unless otherwise specified.
Material used in the embodiment of the present invention, reagent etc., are commercially available unless otherwise specified.
Embodiment 1
A kind of skin care item containing complex polysaccharide series, including following weight percent raw material: sea cucumber polysaccharide 17%, tremella
Polysaccharide 11%, ferulic acid 2.5%, panthenol 0.8%, glycerol 0.7%, hyaluronic acid 1.1%, solubilizer 6%, emulsion stabilizer
7%, preservative 0.3% and balance deionized water.
Embodiment 2
A kind of skin care item containing complex polysaccharide series, including following weight percent raw material: sea cucumber polysaccharide 28%, tremella
Polysaccharide 23%, ferulic acid 5.0%, panthenol 1.2%, glycerol 1.0%, hyaluronic acid 2.5%, solubilizer 19%, emulsion stabilizer
25%, preservative 0.7% and balance deionized water.
Embodiment 3
A kind of skin care item containing complex polysaccharide series, including following weight percent raw material: sea cucumber polysaccharide 19%, tremella
Polysaccharide 13%, ferulic acid 3.1%, panthenol 1.0%, glycerol 0.8, hyaluronic acid 1.5%, solubilizer 7%, emulsion stabilizer
10%, preservative 0.3% and balance deionized water.
Embodiment 4
A kind of skin care item containing complex polysaccharide series, including following weight percent raw material: sea cucumber polysaccharide 25%, tremella
Polysaccharide 19%, ferulic acid 4.7%, panthenol 1.2%, glycerol 1.0%, hyaluronic acid 2%, solubilizer 17%, emulsion stabilizer
21%, preservative 0.6% and balance deionized water.
Embodiment 5
A kind of skin care item containing complex polysaccharide series, including following weight percent raw material: sea cucumber polysaccharide 21%, tremella
Polysaccharide 15%, ferulic acid 3.8%, panthenol 1.1%, glycerol 0.9%, hyaluronic acid 1.9%, solubilizer 9%, emulsion stabilizer
14%, preservative 0.3% and balance deionized water.
A kind of preparation method of the skin care item containing complex polysaccharide series of above-described embodiment 1~5, comprising the following steps:
The preparation method of the sea cucumber polysaccharide the following steps are included:
S1: crossing 60 meshes for sea cucumber dry powder, be placed in water, and solid-liquid ratio 1:55 obtains sea cucumber dry powder solution；
S2: ultrasonic wave complex enzyme hydrolysis: being added in sea cucumber dry powder solution plus acetic acid-sodium acetate buffer solution adjust pH to
5.0, complex enzyme A, papain, the trypsase, lipase system that the complex enzyme A is 14:25:11 by mass ratio is added
At, ultrasonic wave auxiliary under the action of digested, obtain sea cucumber Thick many candies extracting solution；
S3: ultrasonic wave enzyme deactivation: by sea cucumber Thick many candies extracting solution in 22k Hz, 1W/m L carries out enzyme deactivation under the conditions of 35 DEG C；
S4: extraction: by the sea cucumber Thick many candies extracting solution after enzyme deactivation in 65 DEG C of extraction 70min, extraction 3 times is repeated, is filtered, is closed
And filtrate obtains filtrate, takes supernatant to be filtered after centrifugation；
S5: alcohol precipitation: by filtered sea cucumber polysaccharide leaching liquor be added 6 times of volume 75%v/v ethanol solutions, stand 10h, two
Secondary centrifugation, precipitating is redissolved with distilled water, and is dialysed and removed small molecular weight impurity, is filtered, and crosses 500 meshes, after trapped fluid freeze-drying to obtain the final product
To sea cucumber polysaccharide.
A kind of preparation method of the skin care item containing complex polysaccharide series of above-described embodiment 1~5, comprising the following steps:
The preparation methods of the tremella polysaccharides the following steps are included:
S1: taking tremella appropriate, and cleaning dry, pulverize, and take tremella powder, crosses 70 meshes；
S2: Subcritical water chromotagraphy: Xiang Yiner powder is added water, dodecanoic acid and Pfansteihl, ultrasound-enhanced subcritical water and mentions
It takes, solid-liquid ratio 1:70 obtains tremella polysaccharides extracting solution；
S3: removing protein: being added complex enzyme B in tremella polysaccharides extracting solution, the complex enzyme B is papain, tryptose
Enzyme, lipase, ratio 3:5:2 digest 2.5h under conditions of temperature is 50 DEG C, pH is 8, are repeated 3 times, stratification, point
Sub-cloud denatured protein and intersection organic solvent obtain de- albumen tremella polysaccharides liquid；
S4: alcohol precipitation: being added ethyl acetate and water in de- albumen tremella polysaccharides liquid, extracts, collection water phase, filtering and concentrating,
Concentrate is spray-dried to get tremella polysaccharides.
A kind of preparation method of the skin care item containing complex polysaccharide series of above-described embodiment 1~5, comprising the following steps:
S1: at room temperature, deionized water is added in 20% sea cucumber polysaccharide and tremella polysaccharides, is heated to
It is dissolved at 50 DEG C, obtains solution A；
S2: panthenol, glycerol, hyaluronic acid, emulsion stabilizer are dissolved in 75 DEG C of agitating and heatings, anti-corrosion is added after being cooled to
Agent obtains solution B.
S3: solution A, B are mixed, and ferulic acid, solubilizer, pH adjusting agent, stirring, homogeneous is added.
Embodiment 6
The present embodiment use weight percent raw material same as Example 5, the preparation method of the sea cucumber polysaccharide include with
Lower step:
S1: crossing 40 meshes for sea cucumber dry powder, be placed in water, and solid-liquid ratio 1:40 obtains sea cucumber dry powder solution；
S2: ultrasonic wave complex enzyme hydrolysis: being added in sea cucumber dry powder solution plus acetic acid-sodium acetate buffer solution adjust pH to
4.8, complex enzyme A is added, the complex enzyme A is made of mass ratio of the papain, trypsase, lipase of 12:22:9,
It is digested under the action of ultrasonic wave auxiliary, obtains sea cucumber Thick many candies extracting solution；
S3: ultrasonic wave enzyme deactivation: by sea cucumber Thick many candies extracting solution in 15k Hz, 0.42W/m L goes out under the conditions of 30 DEG C
Enzyme；
S4: extraction: by the sea cucumber Thick many candies extracting solution after enzyme deactivation in 50 DEG C of extraction 60min, extraction 2 times is repeated, is filtered, is closed
And filtrate obtains filtrate, takes supernatant to be filtered after centrifugation；
S5: alcohol precipitation: 4 times of volume 50%v/v ethanol solutions are added in filtered sea cucumber polysaccharide leaching liquor, are stood under room temperature
8h, secondary centrifuging, precipitating is redissolved with distilled water, and is dialysed and removed small molecular weight impurity, is filtered, and 400 meshes is crossed, after trapped fluid freeze-drying
Obtain sea cucumber polysaccharide.
The preparation methods of the tremella polysaccharides the following steps are included:
S1: taking tremella appropriate, cleans, and crushes, and takes tremella powder, crosses 60 meshes；
S2: water, dodecanoic acid and Pfansteihl, ultrasound-enhanced subcritical water Subcritical water chromotagraphy: are added in Xiang Yiner powder
It extracts, solid-liquid ratio 1:50 obtains tremella polysaccharides extracting solution；
S3: removing protein: being added complex enzyme B in tremella polysaccharides extracting solution, the complex enzyme B is papain, tryptose
Enzyme, lipase, ratio 1.1:4.1:1.7 digest 2h under conditions of temperature is 40 DEG C, pH is 7, are repeated 2 times, and stand and divide
Layer, divides sub-cloud denatured protein and intersection organic solvent, obtains de- albumen tremella polysaccharides liquid；
S4: alcohol precipitation:: ethyl acetate and water are added in de- albumen tremella polysaccharides liquid, extracts, collection water phase, filtering and concentrating,
Concentrate is spray-dried to get tremella polysaccharides.
A kind of preparation method of the skin care item containing complex polysaccharide series, comprising the following steps:
S1: at room temperature, deionized water is added in 10% sea cucumber polysaccharide and tremella polysaccharides, is heated to
It is dissolved at 40 DEG C, obtains solution A；
S2: panthenol, glycerol, hyaluronic acid, emulsion stabilizer are dissolved in 70 DEG C of agitating and heatings, anti-corrosion is added after being cooled to
Agent obtains solution B；
S3: solution A, B are mixed, and ferulic acid, solubilizer, pH adjusting agent, stirring, homogeneous is added.
Embodiment 7
The present embodiment use weight percent raw material same as Example 5, the preparation method of the sea cucumber polysaccharide include with
Lower step:
S1: crossing 80 meshes for sea cucumber dry powder, be placed in water, and solid-liquid ratio 1:70 obtains sea cucumber dry powder solution；
S2: ultrasonic wave complex enzyme hydrolysis: being added in sea cucumber dry powder solution plus acetic acid-sodium acetate buffer solution adjust pH to
5.2, complex enzyme A, papain, the trypsase, lipase system that the complex enzyme A is 15:28:13 by mass ratio is added
At, ultrasonic wave auxiliary under the action of digested, obtain sea cucumber Thick many candies extracting solution；
S3: ultrasonic wave enzyme deactivation: by sea cucumber Thick many candies extracting solution in 28k Hz, 1.05W/m L goes out under the conditions of 44 DEG C
Enzyme；
S4: extraction: by the sea cucumber Thick many candies extracting solution after enzyme deactivation in 80 DEG C of extraction 80min, extraction 4 times is repeated, is filtered, is closed
And filtrate obtains filtrate, takes supernatant to be filtered after centrifugation；
S5: alcohol precipitation: 7 times of volume 95%v/v ethanol solutions are added in filtered sea cucumber polysaccharide leaching liquor, are stood under room temperature
12h, secondary centrifuging, precipitating is redissolved with distilled water, and is dialysed and removed small molecular weight impurity, is filtered, and 600 meshes, trapped fluid freeze-drying are crossed
Sea cucumber polysaccharide is obtained afterwards.
The preparation methods of the tremella polysaccharides the following steps are included:
S1: taking tremella appropriate, cleans, and crushes, and takes tremella powder, crosses 80 meshes；
S2: Subcritical water chromotagraphy: in Xiang Yiner powder plus water, dodecanoic acid and Pfansteihl, ultrasound-enhanced subcritical water mention
It takes, solid-liquid ratio 1:80 obtains tremella polysaccharides extracting solution；
S3: removing protein: being added complex enzyme B in tremella polysaccharides extracting solution, the complex enzyme B is papain, tryptose
Enzyme, lipase, ratio 3.5:5.3:2.5 digest 3h under conditions of temperature is 60 DEG C, pH is 9, are repeated 4 times, and stand and divide
Layer, divides sub-cloud denatured protein and intersection organic solvent, obtains de- albumen tremella polysaccharides liquid；
S4: alcohol precipitation: being added ethyl acetate and water in de- albumen tremella polysaccharides liquid, extracts, collection water phase, filtering and concentrating,
Concentrate is spray-dried to get tremella polysaccharides.
The present embodiment uses weight percent raw material same as Example 5, a kind of shield containing complex polysaccharide series
The preparation method of skin product, comprising the following steps:
S1: at room temperature, deionized water is added in 30% sea cucumber polysaccharide and tremella polysaccharides, is heated to molten at 60 DEG C
Solution, obtains solution A；
S2: panthenol, glycerol, hyaluronic acid, emulsion stabilizer are dissolved in 80 DEG C of agitating and heatings, anti-corrosion is added after being cooled to
Agent obtains solution B.
S3: solution A, B are mixed, and ferulic acid, solubilizer, pH adjusting agent, stirring, homogeneous is added.
Comparative example 1
The difference of this comparative example and embodiment 5 is, in a kind of raw material of the skin care item containing complex polysaccharide series not
Contain sea cucumber polysaccharide.
Comparative example 2
The difference of this comparative example and embodiment 5 is, in a kind of raw material of the skin care item containing complex polysaccharide series not
Contain tremella polysaccharides.
Comparative example 3
This comparative example is with the difference of embodiment 5: a kind of skin care item containing complex polysaccharide series, including following heavy
Measure percentage raw material: sea cucumber polysaccharide 9%, tremella polysaccharides 2%, ferulic acid 0.1%, panthenol 0.1%, glycerol 0.3%, hyaluronic acid
0.4%, solubilizer 4%, emulsion stabilizer 5%, preservative 0.01% and balance deionized water.
By containing complex polysaccharide series made from the embodiment of the present invention 1~7 and comparative example 1~3 skin care item and city
Certain moisturizing anti-aging skin care product carries out efficacy test on face, and test result is as follows:
One, moisture-keeping efficacy measures
25 years old~50 years old volunteer 80 of tissue (male, each 40 of female) is randomly divided into 10 groups, experimental period 12h, temperature
For (23 ± 2) DEG C, humidity is 40%~50%.In the left and right inboard arm defined area of every volunteer, label is tested respectively
Position (4cm × 4cm), test sample and control are randomly dispersed on left and right arm.Smear 0.05mL sample in each tested position
Product measure 5 period test sample regions and control zone skin moisture content using Multi-functional skin tester after smearing,
All original moisture content of skin of experimenter are 25~28%.
The skin care item of Examples 1 to 7 and comparative example 1~3 containing complex polysaccharide series try out skin moisture content table (%)
| Time (h) | 2 | 4 | 8 | 10 | 12 |
| Embodiment 1 | 53.4 | 50.7 | 43.3 | 42.6 | 34.5 |
| Embodiment 2 | 54.1 | 50.4 | 44.8 | 40.1 | 37.9 |
| Embodiment 3 | 58.5 | 53.4 | 47.0 | 42.3 | 38.5 |
| Embodiment 4 | 59.5 | 55.4 | 50 | 42.4 | 38.2 |
| Embodiment 5 | 61.5 | 58.8 | 54.2 | 48.1 | 40.0 |
| Embodiment 6 | 58.9 | 55.4 | 46.1 | 42.3 | 37.5 |
| Embodiment 7 | 57.2 | 52.4 | 47.1 | 43.3 | 38.1 |
| Comparative example 1 | 36.9 | 34.3 | 32.1 | 29.9 | 28.6 |
| Comparative example 2 | 39.8 | 36.8 | 31.2 | 28.3 | 27.0 |
| Comparative example 3 | 40.1 | 35.1 | 32.0 | 30.1 | 29.7 |
| Reference substance | 35.8 | 33.9 | 32.1 | 30.2 | 28.5 |
The result shows that: skin care item skin moisture content in 12 hours containing complex polysaccharide 34.5%~61.5% it
Between, and in control group, as time increases, moisture is gradually reduced to 30% hereinafter, in embodiment 6~7, due to complex enzyme
Ratio and parameter setting, influence the recovery rate and purity of polysaccharide, have certain shadow to the skin care item moisture retention of post-production
It rings；Lack any polysaccharide in comparative example 1~2, skin moisture content is 30% hereinafter, cannot make sea cucumber polysaccharide and tremella polysaccharides
In conjunction with playing better moisture retention；Outside range, skin care item made from same method exist all components proportion in comparative example 3
Moisture retention fall flat after 12h, reference substance restore the water content of original skin after 12h；Then the present invention is containing multiple
The skin care item for closing polysaccharide have preferable moistening effect.
Two, senile-resistant efficacy is tested
(1) experimental group: selection 30 years old~55 years old volunteer, 55 women are randomly divided into 11 groups, and every group of 5 people, each group is in skin
Skin health status, collective's endocrine conditions etc. there are no significant difference.
(2) application method: being (23 ± 2) DEG C in temperature, humidity is 40%~50%.This is used in left facial part sooner or later daily
Anti-aging skin care product is each primary, uses observation surrounding.
(3) evaluation criterion: 1, trier evaluates autologous skin change；Every using effect total score in grade form
It is 10 points, 9~10 points of expression effects are best, and 6~8 points of expression effects are preferable, and 4~5 points of expression effects are general, 3 points of following presentations
Effect is poor, and every using effect average is as follows:
Evaluation result: in the scoring that skin aging improves that degree and pachylosis improve this two indexs of degree, implement
3~7 comprehensive satisfaction of example reaches 9 points or more, and in embodiment 3~5, each group of score value progressivelyes reach the optimum value of efficacy exertion；
The complex enzyme of different proportion is arranged in range in the component ratio of embodiment 6~7, so that the enzymatic hydrolysis of polysaccharide and deproteinized generate
Effect preference, parameter including equipment etc. influences, so that the series skin nursing products effect containing complex polysaccharide of preparation is comprehensive full
Meaning degree reaches 9 points or more；For each component in value range, complex enzyme reaches optimal effect by scientific matching in embodiment 5；
1~3 comprehensive satisfaction of comparative example lacks any polysaccharide in 8 points or more, comparative example 1~2, and effect is substantially reduced；Reference substance tool
There is certain senile-resistant efficacy, comprehensive satisfaction is at 6 points or more.
The result shows that moisture retention is very significant after using the skin care item of the invention containing complex polysaccharide, sea cucumber polysaccharide with
Its unique molecular structure and the effect of the infiltration skin of physicochemical property are lower so that showing in conjunction with the humectant of tremella polysaccharides a variety of
The effect of important foster skin skin-protecting function and anti-aging, the moisture-keeping functions such as aquation, lubrication can be played, thus moisturize the skin,
And the factors such as dry skin can be inhibited.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of skin care item containing complex polysaccharide series, it is characterised in that: including following weight percent raw material: sea cucumber polysaccharide
17%~28%, tremella polysaccharides 11%~23%, ferulic acid 2.5%~5.0%, panthenol 0.8~1.2%, glycerol 0.7~
1.0%, hyaluronic acid 1.1%~2.5%, solubilizer 6%~19%, emulsion stabilizer 7%~25%, preservative 0.3%~
0.7% and balance deionized water.
2. a kind of skin care item containing complex polysaccharide series, it is characterised in that: including following weight percent raw material: sea cucumber polysaccharide
19%~25%, tremella polysaccharides 13%~19%, ferulic acid 3.1%~4.7%, panthenol 1.0~1.2%, glycerol 0.8~
1.0%, hyaluronic acid 1.5%~2%, solubilizer 7%~17%, emulsion stabilizer 10%~21%, preservative 0.3%~
0.6% and balance deionized water.
3. a kind of skin care item containing complex polysaccharide series, it is characterised in that: including following weight percent raw material: sea cucumber polysaccharide
21%, tremella polysaccharides 15%, ferulic acid 3.8%, panthenol 1.1%, glycerol 0.9%, hyaluronic acid 1.9%, solubilizer 9%, cream
Change stabilizer 14%, preservative 0.3% and balance deionized water.
4. a kind of skin care item containing complex polysaccharide series according to claim 1, which is characterized in that the sea cucumber polysaccharide
Preparation method the following steps are included:
S1: crossing 40~80 meshes for sea cucumber dry powder, be placed in water, and solid-liquid ratio 1:40~70 obtain sea cucumber dry powder solution；
S2: ultrasonic wave complex enzyme hydrolysis: being added in sea cucumber dry powder solution plus acetic acid-sodium acetate buffer solution adjust pH to 4.8~
5.2, complex enzyme A is added, the complex enzyme A is 12~15:22~28:9~13 papain, tryptose by mass ratio
Enzyme, lipase are made, and are digested under the action of ultrasonic wave auxiliary, obtain sea cucumber Thick many candies extracting solution；
S3: ultrasonic wave enzyme deactivation: by sea cucumber Thick many candies extracting solution in 15~28k Hz, 0.42~1.05W/m L, 30 DEG C~45 DEG C items
Enzyme deactivation is carried out under part；
S4: extraction: by the sea cucumber Thick many candies extracting solution after enzyme deactivation in 50 DEG C~80 DEG C 60~80min of extraction, extraction 2~4 is repeated
Secondary, filtering, merging filtrate obtains filtrate, takes supernatant to be filtered after centrifugation；
S5: alcohol precipitation: 4~7 times of volume 50%~95%v/v ethanol solutions are added in filtered sea cucumber polysaccharide leaching liquor, stand 8
~12h, secondary centrifuging, precipitating is redissolved with distilled water, and is dialysed and removed small molecular weight impurity, is filtered, and 400~600 meshes, retention are crossed
Sea cucumber polysaccharide is obtained after liquid freeze-drying.
5. a kind of skin care item containing complex polysaccharide series according to claim 1, which is characterized in that the tremella polysaccharides
Preparation method the following steps are included:
S1: taking tremella appropriate, and cleaning dry, pulverize, and take tremella powder, crosses 60~80 meshes；
S2: Subcritical water chromotagraphy: water, dodecanoic acid and Pfansteihl, ultrasound-enhanced Subcritical water chromotagraphy, material is added in Xiang Yiner powder
Liquor ratio 1:50~80 obtain tremella polysaccharides extracting solution；
S3: removing protein: being added complex enzyme B in tremella polysaccharides extracting solution, the complex enzyme B is papain, trypsase, rouge
Fat enzyme, ratio are 1.1~3.5:4.1~5.3:1.7~2.5, digest 2 under conditions of temperature is 40~60 DEG C, pH is 7~9
~3h is repeated 2~4 times, and stratification divides sub-cloud denatured protein and intersection organic solvent, and it is more to obtain de- albumen tremella
Liquid glucose；
S4: alcohol precipitation: being added ethyl acetate and water in de- albumen tremella polysaccharides liquid, and water phase, filtering and concentrating, concentration are collected in extraction
Liquid is spray-dried to get tremella polysaccharides.
6. a kind of skin care item containing complex polysaccharide series according to claim 4, are characterized in that, in S1 step 2), institute
The additive amount for stating complex enzyme A is the 0.6~1% of sea cucumber dry powder quality.
7. a kind of skin care item containing complex polysaccharide series according to claim 5, are characterized in that, in S1 step 3), institute
The additive amount for stating complex enzyme B is the 0.5~1.2% of tremella dry powder quality.
8. a kind of skin care item containing complex polysaccharide series according to claim 4, are characterized in that, in S1 step 2), enzyme
Solving temperature is 50 DEG C~60 DEG C, and ultrasonic power is 400~600W, and 1.5~2h of time, frequency is 25~28kHz.
9. a kind of preparation method of the skin care item containing complex polysaccharide series, feature described according to claim 1~8 exist
In, comprising the following steps:
S1: at room temperature, being added deionized water for 10%~30% sea cucumber polysaccharide and tremella polysaccharides, it is again heated to 40~
It is dissolved at 60 DEG C, obtains solution A；
S2: panthenol, glycerol, hyaluronic acid, emulsion stabilizer are dissolved in 70~80 DEG C of agitating and heatings, anti-corrosion is added after being cooled to
Agent obtains solution B.
S3: solution A, B are mixed, and ferulic acid, solubilizer, pH adjusting agent, stirring, homogeneous is added.
10. a kind of skin care item containing complex polysaccharide series described according to claim 1~any one of 8, it is characterised in that:
The skin care item are one of toner, lotion, face cream, eye cream, facial mask.
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Application publication date: 20190326 |