CN1095102A - The foundation of model of producing beta-carotene by cultivating marine algae - Google Patents
The foundation of model of producing beta-carotene by cultivating marine algae Download PDFInfo
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- CN1095102A CN1095102A CN 93106062 CN93106062A CN1095102A CN 1095102 A CN1095102 A CN 1095102A CN 93106062 CN93106062 CN 93106062 CN 93106062 A CN93106062 A CN 93106062A CN 1095102 A CN1095102 A CN 1095102A
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- luobusu
- carotene
- pattern
- producing beta
- cumulative
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Abstract
The invention belongs to the producing beta-carotene by cultivating marine algae biological technical field.
Culture at present the salt algae and carry out by rule of thumb, blindness is big, not science very.Synthetic study salt algae accumulates β-Hu Luobusu in a large number, scientifically sets up producing beta-carotene by cultivating marine algae and optimizes pattern, instructs practice, becomes the key technical problem in this field.
For solving this technical problem, design of the present invention is: study various environmental factors to dunaiella salina growth, β-Hu Luobusu and the influence of isomer cumulative thereof, find out and be suitable for dunaiella salina growth, β-Hu Luobusu cumulative envrionment conditions, analysis-by-synthesis is set up pattern, instruct and produce, improve output.
Description
The invention belongs to the producing beta-carotene by cultivating marine algae biological technical field.
β-Hu Luobusu is provitamin A, good food dye, novel anti-cancer, anticancer and delay senility, reduce the medicine of apoplexy and evidence of coronary heart diseases, has broad application prospects.The salt algae is that natural beta-carotin is a best resource.Producing beta-carotene by cultivating marine algae becomes focus and newborn biotechnology of the little algae circle research in various countries.Culture at present the salt algae method and still carry out by rule of thumb, have blindness, not science very.The condition that synthetic study salt algae accumulates β-Hu Luobusu is in a large number scientifically set up producing beta-carotene by cultivating marine algae and is optimized pattern, instructs practice, becomes the key technical problem in this field.
For solving this technical problem, design of the present invention is: study various environmental factors to dunaiella salina growth, the influence of β-Hu Luobusu cumulative, find out and be suitable for dunaiella salina growth, β-Hu Luobusu cumulative envrionment conditions, analysis-by-synthesis is set up pattern, instruct and produce, improve output.
Feature of the present invention is: the optimization pattern, be suitable for dunaiella salina growth, β-Hu Luobusu accumulation and cis-isomeride cumulative pattern, foundation is suitable for dunaiella salina growth, β-Hu Luobusu cumulative pattern, instructs the practice in the producing beta-carotene by cultivating marine algae.
Advantage of the present invention is: (1) two pattern is to be based upon on the basis of big quantity research its science and good reliability; (2) purpose is strong: use the pattern that is suitable for dunaiella salina growth, can in the unit time, obtain maximum biomass, and favourable to producing protein feed; Application is suitable for β-Hu Luobusu cumulative pattern, can obtain maximum β-Hu Luobusu in the unit time.
For realizing the present invention, at first study various environmental factors (as light, temperature, salt, nitrogen, phosphorus, iron, bittern, bittern water etc.) to dunaiella salina growth, the influence of β-Hu Luobusu cumulative, find out and be suitable for dunaiella salina growth, β-Hu Luobusu cumulative envrionment conditions, analysis-by-synthesis, the electronics machine is calculated the machine manual simulation, sets up the optimization pattern.Wherein, 1. the pattern of dunaiella salina growth is: intensity of illumination ≈ 30000 luxs; Salinity is 150～180 ‰; Nutrient solution need not sterilized; Temperature is at 24～28 ℃; N is a nitrate nitrogen, and concentration is at 1mmol/L, and P is KH
2PO
4The time, concentration is at 0.3mmol/L; Replenish inorganic carbon source, if select NaHCO
3, if 1g/L then is CO
2, then the pH value can not be too low, is preferably between 7～9; Add molysite, particularly ironic citrate; Add trace element; Add bittern water, in 0～75% scope, The more the better.
2. β-Hu Luobusu cumulative pattern is: intensity of illumination is greater than 30000 luxs, and is high more good more; Salinity is 240 ‰; Nutrient solution need not sterilized; Temperature is more than 30 ℃; N is a nitrate nitrogen, and concentration is at 0.5～1mmol/L, and P is KH
2PO
4The time, concentration is at 0.1mmol/L; Replenish inorganic carbon source, if select NaHCO
3, if 1g/L then is CO
2, then the pH value can not be too low, is preferably between 7～9; Add molysite, particularly ironic citrate; Add trace element; Add bittern water, in 18.5 or 75% fashion.
Claims (3)
1, optimization pattern is suitable for dunaiella salina growth, β-Hu Luobusu cumulative pattern, sets up dunaiella salina growth, the β-Hu Luobusu cumulative pattern of being suitable for, and instructs and cultures the salt algae.
2, according to claim 1, utilize the pattern that is suitable for dunaiella salina growth, instruct producing beta-carotene by cultivating marine algae.
3, according to claim 1, utilize and be suitable for β-Hu Luobusu cumulative pattern, instruct the practice in the producing beta-carotene by cultivating marine algae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93106062 CN1095102A (en) | 1993-05-09 | 1993-05-09 | The foundation of model of producing beta-carotene by cultivating marine algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93106062 CN1095102A (en) | 1993-05-09 | 1993-05-09 | The foundation of model of producing beta-carotene by cultivating marine algae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1095102A true CN1095102A (en) | 1994-11-16 |
Family
ID=4986052
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93106062 Pending CN1095102A (en) | 1993-05-09 | 1993-05-09 | The foundation of model of producing beta-carotene by cultivating marine algae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1095102A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102605032A (en) * | 2012-04-13 | 2012-07-25 | 暨南大学 | Method for producing beta-carotene by Eustigmatoscf. polyphem |
| CN104664165A (en) * | 2015-03-11 | 2015-06-03 | 任芳芳 | Natural pigment feed additive for freshwater aquaculture of vannamei |
| CN104711195A (en) * | 2015-04-02 | 2015-06-17 | 丁河峰 | Dunaliella culture method |
| CN105176825A (en) * | 2015-10-13 | 2015-12-23 | 中盐工程技术研究院有限公司 | Method for large-scale production of dunaliella salina by means of sodium sulfate salt lake and salt pan halogen pond |
-
1993
- 1993-05-09 CN CN 93106062 patent/CN1095102A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102605032A (en) * | 2012-04-13 | 2012-07-25 | 暨南大学 | Method for producing beta-carotene by Eustigmatoscf. polyphem |
| CN104664165A (en) * | 2015-03-11 | 2015-06-03 | 任芳芳 | Natural pigment feed additive for freshwater aquaculture of vannamei |
| CN104711195A (en) * | 2015-04-02 | 2015-06-17 | 丁河峰 | Dunaliella culture method |
| CN105176825A (en) * | 2015-10-13 | 2015-12-23 | 中盐工程技术研究院有限公司 | Method for large-scale production of dunaliella salina by means of sodium sulfate salt lake and salt pan halogen pond |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |