CN109504600A - A kind of constant temperature oscillation incubating device suitable for liquor removing workstation - Google Patents
A kind of constant temperature oscillation incubating device suitable for liquor removing workstation Download PDFInfo
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- CN109504600A CN109504600A CN201811513365.9A CN201811513365A CN109504600A CN 109504600 A CN109504600 A CN 109504600A CN 201811513365 A CN201811513365 A CN 201811513365A CN 109504600 A CN109504600 A CN 109504600A
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- Prior art keywords
- constant temperature
- oscillation
- temperature oscillation
- slide unit
- liquor removing
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- 230000010355 oscillation Effects 0.000 title claims abstract description 56
- 230000007246 mechanism Effects 0.000 claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims description 18
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 7
- 229910052782 aluminium Inorganic materials 0.000 claims description 7
- 239000004411 aluminium Substances 0.000 claims description 7
- 238000009413 insulation Methods 0.000 claims description 6
- 230000035939 shock Effects 0.000 claims description 5
- 239000004519 grease Substances 0.000 claims description 2
- 229920001296 polysiloxane Polymers 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 7
- 238000012546 transfer Methods 0.000 abstract description 7
- 238000011534 incubation Methods 0.000 abstract description 2
- 239000012472 biological sample Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 239000011324 bead Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000010876 biochemical test Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kind of constant temperature oscillation incubating devices suitable for liquor removing workstation, including constant temperature oscillation mechanism and auxiliary body；Constant temperature oscillation mechanism includes oscillation module and thermostat module, and the oscillation module includes firm banking, linear motor, motor shaft, the first fixed station, the second fixed station, middle slide unit, upper slide unit, capacity eccentric bearing, upper slide bar, lower litter, self-lubricating bush, oscillating table；The thermostat module is arranged above oscillation module and can place microwell plate, and auxiliary body includes cabinet, places micropore board mount on cabinet.The device drives capacity eccentric bearing rotation by linear motor, and capacity eccentric bearing drives slide unit to move back and forth along slide bar, so that oscillating table be driven to realize oscillation functions.The device can cooperate the mechanical gripper and Multi-channel liquid transfer device of liquor removing workstation, realize automatic transfer of the microwell plate between constant temperature oscillation incubating device and liquor removing workstation, while realize full-automatic high-throughput biological sample constant-temperature incubation operation.
Description
Technical field
The present invention relates to a kind of constant temperature oscillation incubating devices, and in particular to a kind of constant temperature oscillation suitable for liquor removing workstation
Incubating device.
Background technique
Constant temperature oscillation incubation is commonly operated in biochemical test, and the experiments such as probe modification, nucleic acid hybridization are usually used in.It is common
Constant temperature oscillation device be the biggish incubator type of specification mostly, structure function is single, cannot be integrated in full-automatic liquid relief
In work station, while the access of consumptive material is dependent on manual operations, can not cooperate the mechanical gripper and multichannel of liquor removing workstation
Pipettor realizes automatic transfer of the microwell plate between constant temperature oscillation incubating device and liquor removing workstation, is unfavorable for realizing experiment
Room automation.
Summary of the invention
Goal of the invention: the object of the present invention is to provide a kind of constant temperature oscillation incubating device, the device adapt to it is all kinds of it is complete from
Dynamic liquor removing workstation, can cooperate the mechanical gripper and Multi-channel liquid transfer device of liquor removing workstation, realize that fully-automatic thermostatic oscillation is incubated
Educate operation.
Technical solution: a kind of constant temperature oscillation incubating device suitable for liquor removing workstation, including constant temperature oscillation mechanism and auxiliary
Help mechanism；The constant temperature oscillation mechanism includes oscillation module and thermostat module, and the oscillation module includes intermediate in groove-like
Firm banking, linear motor are fixedly connected with firm banking, and motor shaft is connected with linear motor, and capacity eccentric bearing is fixed on motor shaft
On, the first fixed station and the second fixed station are fixed on the fixed base, and it is fixed that the lower litter that therefrom slide unit passes through is fixed on first
On platform and the second fixed station, on the side wall that the upper litter passed through from upper slide unit is fixed on firm banking, upper litter and lower litter phase
Mutually vertical, middle slide unit and upper slide unit are in zigzag shape and intermediate hollow out, and capacity eccentric bearing passes through the hollow part of upper slide unit, with upper slide unit
Top flushes, and the groove having among the protrusion and oscillating table that capacity eccentric bearing upper end has matches, and oscillating table and upper slide unit are solid
Fixed connection；Microwell plate can be placed in thermostat module；Auxiliary body includes cabinet, and box house is hollow structure, and top of the box is put
Micropore board mount is set, cabinet is connected with constant temperature oscillation mechanism.
The utility model has the advantages that (1) present invention is fully functional, whole device can be directly integrated in full-automatic liquor removing workstation,
Functional entity can both place all kinds of consumptive materials, can also have heating and oscillation mixing effect.(2) present invention is simply small in structure
Oscillation functions needed for test can be fully achieved in the case where skilful, oscillation module and thermostat module can both work independently mutually not
Interference, can also work simultaneously.(3) present invention can cooperate the mechanical gripper and Multi-channel liquid transfer device of liquor removing workstation,
Realize automatic transfer of the microwell plate between constant temperature oscillation incubating device and liquor removing workstation.(4) present invention has independent control
Unit processed both can be used as independent compact apparatus and use, and was integrated into the dress such as large-scale biochemical analyzer with also can be convenient
It sets, meets the various actual demands of user.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention；
Fig. 2 is the top cross-sectional view of constant temperature oscillation mechanism of the invention；
Fig. 3 is the main view sectional view of constant temperature oscillation mechanism of the invention；
Fig. 4 is monnolithic case figure of the invention；
Fig. 5 is the structural schematic diagram of thermostat module of the invention；
Fig. 6 is the monnolithic case figure that microwell plate is added in the present invention.
Specific embodiment
As shown in figs. 1 and 4, a kind of constant temperature oscillation incubating device of the present invention, including constant temperature oscillation mechanism and auxiliary
Mechanism, such as Fig. 2-3, constant temperature oscillation mechanism includes oscillation module 101 and thermostat module 102.Oscillation module 101 includes intermediate in recessed
The firm banking 201 of channel-shaped, linear motor 202 are fixedly connected with firm banking 201, motor shaft 203 and 202 phase of linear motor
Even, capacity eccentric bearing 208 is fixed on motor shaft, and the first fixed station 204 and the second fixed station 205 are fixed on the fixed base, in
Slide unit 206 and upper slide unit 207 are in zigzag shape and intermediate hollow out, and 2 upper litter 209 pass through upper slide unit 207, middle slide unit 206
2 self-lubricating bush 213,2 upper litter 209 can be equipped in four side walls from self-lubricating cylinder set across 2 lower litter 210
It is fixedly connected respectively with the first fixed station 204 and the second fixed station 205 across self-lubricating bush 213,2 lower litter 210 and 2 piece
Upper litter 209 is mutually perpendicular to, and is arranged in groined type.Capacity eccentric bearing 208 passes through the hollow part of upper slide unit 207, pushes up with upper slide unit 207
Portion flushes, and capacity eccentric bearing is fixedly connected with oscillating table, is specifically as follows two cylindrical protrusions and vibration of 208 upper end of capacity eccentric bearing
It swings two cylinder shape grooves among platform 214 to match, oscillating table 214 is fixedly connected with upper slide unit 207 by 4 screws.Vibration
Swinging module further includes shock structure 211, and shock structure 211 shares 6, is rubber material, bottom end is screwed in firm banking
On 201, the first fixed station 204, the second fixed station 205 are screwed with the top of 3 shock structures 211 connect respectively, damping
The effect of column 211 is to buffer the vibration generated from the work of upper layer mechanism, ensures that the stabilization of pedestal and whole device
Property.Oscillation module further includes configuration supporting block 209, and counterweight supporting block 209 is located at 208 lower section of capacity eccentric bearing and is fixed on motor shaft
On, counterweight supporting block 209 is rotated together with capacity eccentric bearing 208, and always in the another side of capacity eccentric bearing 208, it is ensured that straight line
The stability of whole device when 202 high speed rotation of motor.
Such as Fig. 5, thermostat module 102 specifically includes heat-conducting plate 301, thermal insulation board 302, heating plate 303, the bottom of thermal insulation board 302
Portion is fixedly connected with oscillating table 214, and heating plate 303 is fixedly connected with thermal insulation board 302, heat-conducting plate 301 respectively, outside heat-conducting plate 301
It is with the outer thermal-insulated frame of a circle, there are two protrusions for outer thermal-insulated frame four edges each edge, for fixing microwell plate.Heating plate 303 is aluminium
Base resistance heating plate, surface are welded with 132 Chip-Rs, by changing the resistance value size of resistance, can arbitrarily change heating
The heating power of plate, and heating power can accurately calculate.Aluminium base resistance heating plate surface is also integrated with a PT100 temperature-sensitive
Resistance, for the temperature of real-time monitoring aluminium base resistance heating plate, as temperature controlled feedback signal, thus to heating module
Accurate temperature controller.It is coated with heat-conducting silicone grease between aluminium base resistance heating plate and heat-conducting plate, and is fitted closely by screw, to improve heat
Conduction of velocity.
Such as Fig. 6, auxiliary body includes three cabinets 103, and box house is hollow structure, and microwell plate branch is placed in top of the box
Frame, microwell plate can be arbitrarily placed in above according to actual needs, and motor driver and control circuit board, cabinet is arranged in box cavity
Mechanism is connected with constant temperature oscillation.
The present invention cooperates the mechanical gripper and Multi-channel liquid transfer device of liquor removing workstation, realizes that microwell plate is incubated in constant temperature oscillation
Specific step is as follows for shifting between device and liquor removing workstation automatically:
(1) prepared by test specimen, prepares the magnetic bead of probe modification, extracts and expand NDA product to be hybridized.
(2) magnetic bead, the hybridization of probe modification is added in microwell plate with eight channel pipettors of full-automatic liquor removing workstation
Liquid, NDA product and pure water.
(3) microwell plate equipped with sample is transferred to constant temperature of the invention using the mechanical gripper of full-automatic liquor removing workstation
On oscillating mechanism, carries out constant temperature oscillation and is incubated for operation, operating process is as follows:
A) temperature --- 95 DEG C；Time --- 5 minutes；202 revolving speed 30rpm of linear motor；
B) temperature --- 55 DEG C；Time --- 60 minutes；202 revolving speed 15rpm of linear motor.
(4) after the reaction was completed, cleaning solution is added, carries out cleaning operation twice, cleaning oscillation every time mixes 3 minutes, straight line
202 revolving speed 60rpm of motor.After the completion of mixing, microwell plate is transferred to full-automatic liquor removing workstation and carries out Magneto separate by mechanical gripper
Supernatant is gone to operate.
(5) after the completion of cleaning operation, microwell plate is transferred to constant temperature oscillation mechanism again, confining liquid is added, shakes at room temperature
It swings and mixes half an hour, 202 revolving speed 30rpm of linear motor is then transferred to magnetic separation mechanism progress Magneto separate and supernatant is gone to operate.
(6) hybrid process is completed, and is collected sample and is carried out subsequent detection operation.
Claims (10)
1. a kind of constant temperature oscillation incubating device suitable for liquor removing workstation, it is characterised in that: including constant temperature oscillation mechanism and auxiliary
Help mechanism；The constant temperature oscillation mechanism includes oscillation module (101) and thermostat module (102), and the oscillation module (101) includes
Centre is in the firm banking (201) of groove-like, and linear motor (202) is fixedly connected with firm banking (201), motor shaft (203)
It is connected with linear motor (202), capacity eccentric bearing (208) is fixed on motor shaft (203), and the first fixed station (204) is solid with second
Determine platform (205) to be fixed on firm banking (201), the lower litter (210) that therefrom slide unit (206) passes through is fixed on the first fixed station
(204) and on the second fixed station (205), the upper litter (209) passed through from upper slide unit (207) is fixed on firm banking (201)
On side wall, upper litter (209) is mutually perpendicular to lower litter (210), middle slide unit (206) and upper slide unit (207) in zigzag shape and
Intermediate hollow out, capacity eccentric bearing (208) pass through the hollow part of upper slide unit (207), flush with upper slide unit (207) top, capacity eccentric bearing
(208) it is fixedly connected with oscillating table (214), oscillating table (214) is fixedly connected with upper slide unit (207)；It is arranged on oscillation module
The thermostat module of side can place microwell plate；Auxiliary body includes cabinet (103), is hollow structure, cabinet top inside cabinet (103)
Micropore board mount (104) are placed in portion, and cabinet (103) is connected with constant temperature oscillation mechanism.
2. the constant temperature oscillation incubating device according to claim 1 suitable for liquor removing workstation, it is characterised in that: middle slide unit
(206) four side walls are equipped with self-lubricating bush (213), and upper litter (209) passes through from self-lubricating bush (213), lower litter
(210) self-lubricating bush (213) are passed through to be fixedly connected with the first fixed station (204) and the second fixed station (205) respectively.
3. the constant temperature oscillation incubating device according to claim 1 suitable for liquor removing workstation, it is characterised in that: the perseverance
Warm module (102) includes thermal insulation board (302), heating plate (303), heat-conducting plate (301), the bottom of thermal insulation board (302) and oscillating table
(214) it is fixedly connected, heating plate (303) is fixedly connected with thermal insulation board (302), heat-conducting plate (301) respectively；The cabinet (103)
It is intracavitary to be equipped with motor driver and control circuit board.
4. the constant temperature oscillation incubating device according to claim 1 suitable for liquor removing workstation, it is characterised in that: the vibration
Swinging module further includes shock structure (211), and shock structure (211) bottom end is fixed on firm banking (201) using screw, and top uses
Screw is fixedly connected with the first fixed station (204), the second fixed station (205) respectively.
5. the constant temperature oscillation incubating device according to claim 1 suitable for liquor removing workstation, it is characterised in that: eccentric shaft
The groove that holding has among the protrusion and oscillating table (214) that (208) upper end has matches.
6. the constant temperature oscillation incubating device according to claim 1 suitable for liquor removing workstation, it is characterised in that: on described
Litter (209) and lower litter (210) have 2, arrange in well word.
7. the constant temperature oscillation incubating device according to claim 1 suitable for liquor removing workstation, it is characterised in that: the vibration
Swinging module further includes configuration supporting block (212), and counterweight supporting block (212) is located at below capacity eccentric bearing (208) and is fixed on motor
On axis (203).
8. the constant temperature oscillation incubating device according to claim 1 suitable for liquor removing workstation, it is characterised in that: heat-conducting plate
(301) there are also outer thermal-insulated frame (304) for periphery.
9. the constant temperature oscillation incubating device according to claim 8 suitable for liquor removing workstation, it is characterised in that: described outer
There are two protrusion (305) on the every side of four edges of thermal-insulated frame (304).
10. the constant temperature oscillation incubating device according to claim 1 suitable for liquor removing workstation, it is characterised in that: described
Heating plate (303) is aluminium base resistance heating plate, and aluminium base resistance heating plate surface has Chip-R and temperature sensor, aluminium base resistance
Heat-conducting silicone grease is coated between heating plate and heat-conducting plate (301).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811513365.9A CN109504600A (en) | 2018-12-11 | 2018-12-11 | A kind of constant temperature oscillation incubating device suitable for liquor removing workstation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811513365.9A CN109504600A (en) | 2018-12-11 | 2018-12-11 | A kind of constant temperature oscillation incubating device suitable for liquor removing workstation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109504600A true CN109504600A (en) | 2019-03-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811513365.9A Pending CN109504600A (en) | 2018-12-11 | 2018-12-11 | A kind of constant temperature oscillation incubating device suitable for liquor removing workstation |
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| Country | Link |
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| CN (1) | CN109504600A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114184778A (en) * | 2021-11-10 | 2022-03-15 | 山东博科生物产业有限公司 | Oscillation incubation device and enzyme-linked immunoassay system |
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| CN2689941Y (en) * | 2003-01-03 | 2005-04-06 | 刘晓民 | Electric quick resolving clevice for solid soluble medicine |
| CN202018457U (en) * | 2011-01-28 | 2011-10-26 | 烟台华上生物科技有限公司 | Micropore plate incubation oscillator |
| CN104117429A (en) * | 2014-07-18 | 2014-10-29 | 东南大学 | Heating, vibrating and magnetic separating device |
| CN206359531U (en) * | 2016-12-30 | 2017-07-28 | 烟台德瑞生物科技有限公司 | Integral type independently shakes incubation carrier |
| CN206369738U (en) * | 2016-09-28 | 2017-08-01 | 中南民族大学 | A kind of constant temperature oscillation incubator |
| CN107983707A (en) * | 2017-10-27 | 2018-05-04 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of board-washing machine with oscillation incubation function |
| DE202018102169U1 (en) * | 2018-04-19 | 2018-05-28 | Chengdu Hongshenglianchuang Technology Co., Ltd. | Oscillation module at constant temperature |
-
2018
- 2018-12-11 CN CN201811513365.9A patent/CN109504600A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2689941Y (en) * | 2003-01-03 | 2005-04-06 | 刘晓民 | Electric quick resolving clevice for solid soluble medicine |
| JP2004290084A (en) * | 2003-03-27 | 2004-10-21 | Sanyo Electric Co Ltd | Shaking apparatus |
| CN202018457U (en) * | 2011-01-28 | 2011-10-26 | 烟台华上生物科技有限公司 | Micropore plate incubation oscillator |
| CN104117429A (en) * | 2014-07-18 | 2014-10-29 | 东南大学 | Heating, vibrating and magnetic separating device |
| CN206369738U (en) * | 2016-09-28 | 2017-08-01 | 中南民族大学 | A kind of constant temperature oscillation incubator |
| CN206359531U (en) * | 2016-12-30 | 2017-07-28 | 烟台德瑞生物科技有限公司 | Integral type independently shakes incubation carrier |
| CN107983707A (en) * | 2017-10-27 | 2018-05-04 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of board-washing machine with oscillation incubation function |
| DE202018102169U1 (en) * | 2018-04-19 | 2018-05-28 | Chengdu Hongshenglianchuang Technology Co., Ltd. | Oscillation module at constant temperature |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114184778A (en) * | 2021-11-10 | 2022-03-15 | 山东博科生物产业有限公司 | Oscillation incubation device and enzyme-linked immunoassay system |
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