CN109498714B

CN109498714B - Angelica sinensis and peony powder for treating inflammatory pain and application thereof

Info

Publication number: CN109498714B
Application number: CN201811559090.2A
Authority: CN
Inventors: 何宏文; 何依帆; 黄芳; 郑苗苗; 李静周
Original assignee: ORAL SUBSIDIARY SUN YAT-SEN UNIVERSITY HOSPITAL
Current assignee: ORAL SUBSIDIARY SUN YAT-SEN UNIVERSITY HOSPITAL
Priority date: 2018-12-19
Filing date: 2018-12-19
Publication date: 2021-06-08
Anticipated expiration: 2038-12-19
Also published as: CN109498714A

Abstract

The invention discloses angelica sinensis and peony powder for treating inflammatory pain and application thereof, wherein the angelica sinensis and peony powder comprises the following medicinal materials in parts by weight: 0-15 parts of angelica: 4-19 parts of peony: 4 parts of poria cocos: 4 parts of bighead atractylodes rhizome: 8 parts of rhizoma alismatis: and 8 parts of ligusticum wallichii. The angelica sinensis and peony powder for treating inflammatory pain has the beneficial effects that the angelica sinensis and peony powder is obtained from the action effect of decoction, is suitable for common dosage forms such as decoction, pills, powder, paste, pellets, medicinal liquor, medicinal granules, oral liquid, capsules, tablets, injections and the like, can effectively treat and relieve various inflammatory pain symptoms of oral cavity, jaw and face, and can also treat and relieve various inflammatory pain symptoms caused by pathological changes of various organs of a body.

Description

Angelica sinensis and peony powder for treating inflammatory pain and application thereof

Technical Field

The invention relates to the technical field of medicines, in particular to angelica sinensis and peony powder for treating inflammatory pain and application thereof.

Background

1. Inflammatory pain of oral and maxillofacial region

Oral and maxillofacial pain is a general term for various types of pain of organs (such as teeth) of oral cavity, maxillofacial region and neck, and is manifested as acute pain and chronic pain. According to the complexity of the oral and maxillofacial anatomical structure, the etiology and clinical characteristics of the disease, the oromaxillofacial pain is divided into inflammatory pain, traumatic pain, tumor pain, joint pain, neuropathic pain, iatrogenic pain, psychological factor pain and the like.

Inflammatory pain of oral and maxillofacial region is pain caused by inflammation of various biological (bacteria, fungi, viruses, etc.), physical, chemical or immunological origin.

On the jaw and oral cavity, the pain caused by inflammation is mainly pulpitis, periapical periodontitis, pericoronitis of wisdom tooth, gap infection, parotitis, submandibular gland inflammation, arthritis, etc. Toothache is one of the most common symptoms in oral clinics. Acute pulpitis, acute periapical periodontitis, pericoronitis, etc. are often manifested as sharp spontaneous pain. Chronic gingivitis and the like often presents as spontaneous dull pain. Dentine hypersensitivity, dental caries of second or third degree, wedge-shaped defects and the like are often manifested as provoking pain.

The inflammatory pain mechanism is complex and is regulated by various factors, and the main mechanism is as follows: 1) local catabolism enhancement of inflammation, inflammatory mediators such as prostaglandin, 5-hydroxytryptamine, bradykinin, and the like, tumor necrosis factor-alpha (TNF-alpha), pain-causing substances, and the like, stimulating nerve endings to cause pain; 2) Inflammatory exudation causes tissue swelling, increased tension, and pain caused by pressing or pulling nerve endings. 3) The pain caused by pulpitis and periapical periodontitis is caused by the fact that the pressure of a pulp cavity is increased and the tissue pressure is increased due to the inflammation of partial or all pulp, the pressing action of the pressure can excite the nerve endings of the C fibers, and the impulse is transmitted to the center to cause the pain.

2. Traditional Chinese medicine view point of toothache

According to the traditional Chinese medicine, the teeth are the rest of bones, the kidney governs bones, and the channels of the foot yangming stomach are in the gum, so that the teeth are closely related to the kidney, the gum and the stomach. From the holistic concept, toothache is often related to invasion of exogenous pathogenic factors, inflammation, dysfunction of liver and kidney, and attention is not paid to self-health care. Generally, acute toothache and red gum are treated by stomach; for chronic toothache, loose teeth, dull and swollen teeth, it is advisable to treat the disease from the kidney. The traditional Chinese medicine mainly divides toothache into the following three types:

1) toothache caused by wind-fire evil is acute, paroxysmal, wind-induced or aggravated, cold-induced pain reduced, heat aggravated, gum red and swollen, and symptoms such as aversion to cold, fever, thirst, rapid pulse and the like are often seen in the early stage of acute pulpitis and periapical periodontitis. For wind-dispelling and heat-clearing, toxicity-removing and swelling-dispersing, the recipe is modified with Yin Qiao powder. Toothache due to stomach fire is severe, gum and face are red and swollen, or gum is overflowed, gum bleeding sometimes causes difficulty in opening mouth, and symptoms such as headache, thirst, halitosis, oliguria, constipation, fever, yellow and greasy tongue coating, etc. are often seen in pericoronitis and suppurative periapical periodontitis. For clearing stomach fire, cooling blood and alleviating pain, it is modified with Qing Wei san. For patients with yin deficiency, cortex Lycii and radix scrophulariae may be added.

2) Toothache caused by deficiency fire has the disadvantages of dull pain of teeth, light degree, aggravation in the afternoon and at night, more and less redness and swelling of gum, loose teeth, no strength of biting materials, aggravation of pain or bleeding of gum. The whole body can be accompanied by symptoms such as soreness of waist, dizziness, dry mouth and throat, red tongue with white coating, and thready and rapid pulse. It is often seen in chronic periodontal disease in the elderly. For nourishing yin and tonifying kidney, decreasing fire and alleviating pain, the formula is modified Zhibai Dihuang Tang.

3) Toothache and traction headache caused by liver fire and toothache, attack or aggravation of mood fluctuation, and general symptoms such as bitter taste in mouth, conjunctival congestion, tinnitus, hypochondriac pain, dysphoria, irritability, red tongue with yellow coating, rapid and forceful pulse, etc. For purging liver and gallbladder, soothing liver and alleviating pain, it is usually combined with Dan Gen Xiegan Tang to remove Mutong and Huang Lian and Dan Pi.

3. Chinese and western medicine view point of dysmenorrhea

Dysmenorrhea is the most common gynecological disease of young women, and refers to abdominal tenesmus, distending pain, waist soreness or other discomfort before or during menstruation, and severe patients with symptoms of cold limbs, chills, nausea, emesis, syncope, etc., which directly affect work, life and learning. Dysmenorrhea is divided into two types according to the existence of organic lesions in reproductive organs, namely primary dysmenorrhea and secondary dysmenorrhea. The primary dysmenorrhea is also called functional dysmenorrhea, most patients have symptoms after menstruation, and the symptoms are mainly manifested by severe pain in the first day of menstruation, improvement after 2-3 days, and disappearance of symptoms after menstruation.

The theory that the increased content of serum prostaglandin causes the strong contraction of uterine wall spiral artery and uterine smooth muscle, and the sensitivity of peripheral nerve to pain is improved by ischemia and hypoxia to cause dysmenorrheal is considered by many scholars as the main mechanism for forming dysmenorrheal. Vasopressin, which is inseparable linked to dysmenorrhea, increases uterine contraction and muscle movement by acting on corresponding receptors on the uterus, resulting in a decrease in uterine blood volume and pain, and increases uterine sensitivity to uterine contraction drugs, which also results in a decrease in blood flow and dysmenorrhea. A dysregulation of the ratio of endothelin and nitric oxide may also be a further important cause of dysmenorrhea.

Traditional Chinese medicine pathogenesis and traditional Chinese medicine theory that the root of dysmenorrhea is in uterus, "stagnation causes pain" and "stagnation causes pain" are main pathogenesis, and deficiency mainly shows qi and blood deficiency, kidney deficiency and other reasons, so that the uterus cannot receive the needed substances, which are called as the stagnation causes pain; the excess is that the body does not lack nutrients, but the blood and qi circulation of the uterus is not smooth due to the obstructed transportation channels, namely qi stagnation and blood stasis, damp-heat stagnation or congealing cold and blood stasis, which is called as "obstruction leading to pain". The traditional Chinese medicine is used for treating primary dysmenorrhea and has definite effect by clinicians and patients.

4. Effect of Danggui Shao powder on pain

The Chinese angelica and peony are scattered from the 'jin Kui Yao L ü e' (during pregnancy of women, abdominal pain, the main part of the Chinese angelica and peony powder). the Chinese angelica and peony powder formula comprises three or two Chinese angelica, one jin of peony, four or two poria cocos, four or two atractylodes macrocephala, half jin of alisma orientale and half jin of ligusticum wallichii, and the six ingredients are added, and the pestle is scattered, the square cun an ancient type of spoon is taken, and the Chinese angelica and peony powder is taken with wine and three days. The raw material is a famous prescription for gynecology, has the functions of nourishing blood, regulating liver, invigorating spleen and promoting diuresis, and is mainly used for treating abdominal pain during pregnancy. On the basis, the later physicians can increase or decrease or adjust the dosage, but the functions are still as follows: nourishing blood, promoting blood circulation, relieving pain, promoting diuresis, regulating liver and softening liver, and can be used for treating autonomic nerve dysfunction, ovarian insufficiency, threatened abortion, chronic nephritis, hypertension, climacteric syndrome, and pelvic inflammatory disease.

However, the study on the use of Danggui Paeonia powder for relieving various pains in oral, maxillofacial and facial areas is rare, so the Danggui Paeonia powder is proposed.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides angelica sinensis and peony powder for treating inflammatory pain and application thereof.

The invention provides angelica sinensis and peony powder for treating inflammatory pain, which comprises the following medicinal materials in parts by weight: 0-15 parts of angelica: 4-19 parts of peony: 4 parts of poria cocos: 4 parts of bighead atractylodes rhizome: 8 parts of rhizoma alismatis: and 8 parts of ligusticum wallichii.

Preferably, the traditional Chinese medicine composition comprises the following medicinal materials in parts by weight: 0 part of Chinese angelica: 19 parts of Chinese herbaceous peony: 4 parts of poria cocos: 4 parts of bighead atractylodes rhizome: 8 parts of rhizoma alismatis: and 8 parts of ligusticum wallichii.

A preparation method of Angelica sinensis and peony powder for treating inflammatory pain comprises the following steps:

s1: selecting high-quality radix Angelicae sinensis, radix Paeoniae, Poria, Atractylodis rhizoma, Alismatis rhizoma and rhizoma Ligustici Chuanxiong according to Chinese medicinal standard;

s2: mixing the angelica, the peony, the tuckahoe, the atractylodes, the rhizoma alismatis and the ligusticum wallichii which are all described in the S1 according to a proportion to obtain a mixture A;

s3: putting the mixture A in the step S2 into a cooking pot, adding a certain volume of distilled water for soaking and decocting, and then carrying out solid-liquid separation to obtain a first decoction dregs B and a first liquid medicine C;

s4: putting the first decoction dregs B in the step S3 into a boiling pot, adding a certain volume of distilled water for decocting, and then carrying out solid-liquid separation to obtain second decoction dregs D and second decoction E;

s5: mixing the second liquid medicine E in S4 with the first liquid medicine C in S3, and concentrating to 1g crude drug/ml to obtain the final product of Angelica sinensis and Paeonia lactiflora powder, and placing the final product of Angelica sinensis and Paeonia lactiflora powder in a refrigerator at 4 deg.C for use, and heating to warm.

Preferably, in S3, the ratio of the volume of the added distilled water to the volume of the mixture a is 8: 1.

preferably, in the step S3, the soaking time is 1 hour, and the decocting time is 2 hours.

Preferably, in the step S4, the volume ratio of the added distilled water to the first medicine residue B is 6: 1.

preferably, in the step S4, the decocting time is 1.5 h.

Preferably, the traditional Chinese medicine composition is prepared into various dosage forms for treating and relieving various inflammatory pains of oral cavity, jaw and face and various inflammatory pains caused by pathological changes of various organs of a body, wherein the dosage forms comprise common dosage forms such as decoction, pills, powder, paste, pills, medicinal liquor, medicinal granules, oral liquid, capsules, tablets, injections and the like.

The invention has the beneficial effects that: the angelica sinensis and peony powder for treating inflammatory pain has the beneficial effects that the angelica sinensis and peony powder is obtained from the action effect of decoction, is suitable for common dosage forms such as decoction, pills, powder, paste, pills, vinum, medicinal granules, oral liquid, capsules, tablets, injection and the like, can effectively treat and relieve various inflammatory pain symptoms of oral cavity, jaw and face, and can also treat and relieve various inflammatory pain symptoms caused by pathological changes of various organs of a body.

Drawings

FIG. 1 is a schematic view of the maxillofacial formalin pain model rat upper lip formalin diffusion zone;

FIG. 2 is a schematic diagram of the prescription of Dang Gui Paeonia lactiflora powder in proportion;

FIG. 3 is a table of the scratching behavior of rats at each time period;

FIG. 4 is a broken line of the rat face scratching times;

FIG. 5 is a graph showing the variation of the number of face scratching for rats with different drugs in a same period of time;

FIG. 6 is a graph of the total number of scratching faces of rats with different drugs.

Detailed Description

The present invention will be further illustrated with reference to the following specific examples.

1. The formulation ratio of Angelica sinensis Paeonia lactiflora powder for treating inflammatory pain is shown in FIG. 2.

2. Preparation method of angelica sinensis and peony powder decoction for treating inflammatory pain

The angelica and peony powder which contain the traditional Chinese medicines are purchased from Guangdong province medicinal material company, and the components and the contents of the angelica and the peony powder are shown in the above chart. The decoction method of the traditional Chinese medicine is as follows: mixing the traditional Chinese medicines according to a certain proportion, adding 8 times of distilled water for soaking for 1h, decocting for 2h, and collecting liquid medicine; adding 6 times volume of distilled water into the residue, decocting for 1.5 hr, and collecting decoction; mixing the two collected medicinal liquids, concentrating to 1g crude drug/ml, and heating in refrigerator at 4 deg.C until it is warm.

3. Method for measuring experimental indexes

(1) Model for treating maxillofacial pain caused by forest disease

All animals were acclimatized for 1 week. Two days before the experiment, animals are continuously habituated to the experimental setting for 30min each day, the cages are transparent organic glass boxes with the length multiplied by the width multiplied by the height =47 multiplied by 25 multiplied by 21cm3, and the experiment is completed under the same illumination and temperature conditions at the same time interval (8: 00-11: 00 in the morning or afternoon). On the day of the experiment, rats were placed in a clear plexiglas chamber 15 minutes before formalin injection. In all experiments, the body and head of the animal were gently fixed with a cloth glove for the same assistant, the same experimenter was quickly inserted with a microsyrin, 2.5% formalin 50 μ l was injected into the subcutaneous tissue of the upper lip on the right side of the rat, and the blank group was injected with an equivalent amount of 0.9% physiological saline. The injection position is shown in figure 1 at the edge of the upper right lip whisker pad, i.e. the junction of the skin and mucosa of the upper lip is injected by needle insertion towards the whisker pad, so that the formalin solution is distributed on the upper lip and the anterior half part of the whisker pad after injection.

(2) Behavioral determination experimental method

All formalin-injected smooth rats were immediately placed in a viewing box for behavioral recording and evaluation. The number of times that rats between different groups use the front/rear claws to scratch the injection part of the right maxillofacial region is respectively recorded as a quantitative index of animal pain by observing for 45 minutes by taking 3min as a unit, and the whole process is recorded. When the timing is observed manually, the whole process of animal pain behaviors is recorded by a high-definition digital HD camera integrated machine, so that later statistical analysis is facilitated.

4. Grouping and measuring metrics

(1) Grouping, grouping

1) And a normal control group: normal mice were drenched with the same amount of normal saline.

2) And pain model group: formalin-induced maxillofacial pain model mouse + drench with equal amount of normal saline.

3) And a sexual control group: the model mouse of the formalin-induced maxillofacial pain + aspirin is 0.1 g/KG.

4) 0:19DSS group: formalin maxillofacial pain model mouse + drench 0: 32DSS 5ml, 10 am each day, for 3 consecutive days.

5) And 1: 9DSS group: formalin maxillofacial pain model mouse + drench 1: 9DSS 5ml, 10 am each day, for 3 consecutive days.

6) And 1: 7DSS group: formalin maxillofacial pain model mouse + drench 1: 7DSS 5ml, 10 am each day, for 3 consecutive days.

7) And 1: 5DSS group: formalin maxillofacial pain model mouse + drench 1: 5DSS 5ml, 10 am each day, for 3 consecutive days.

8) And 1: 3DSS group: formalin maxillofacial pain model mouse + drench 1: 5ml of 3DSS, 10 am each day, for 3 consecutive days.

9) And 1: 1DSS group: formalin maxillofacial pain model mouse + drench 1: 1DSS 5ml, 10 am each day, for 3 consecutive days.

10) And 3: 1DSS group: formalin maxillofacial pain model mouse + drench 3: 1DSS 5ml, 10 am each day, for 3 consecutive days.

11) And 1: 5.33DSS group: formalin maxillofacial pain model mouse + drench 1: 5.33DSS5ml, 10 am each day, for 3 consecutive days.

12) And the original prescription and the flavoring prescription are as follows: the formalin-induced maxillofacial pain model mouse and the original prescription are administrated by 5ml, 10 days in the morning and 3 days in succession.

(2) And the measurement index

Behavioral indicators of rat pain were recorded.

1) And the number of scratching the right maxillofacial injection site by the front/rear claws of the rat is 3min, and the total number of face scratching times is observed for 45 minutes and 15 time periods.

2) Total number of face scrapings in 1 hour in rats.

5. Pain relieving effect of angelica sinensis and peony powder according to prescription proportion

(1) The effects of different drug treatments on the scratching behavior of the formalin-induced maxillofacial pain model mice are shown in fig. 3, 4 and 5.

The proportion of the angelica and peony root prescription drugs from the summary of scratching the face behavior and the stitches removing chart is obviously less than that of the model group, and the proportion of the angelica-free prescription drugs (0: 19DSS group) is reduced most obviously.

As shown in FIG. 6, it can be seen from the total number of face scratching, the ratio of the radix Angelicae sinensis to radix Paeoniae is significantly less than that of the model group, and the reduction is the most obvious with the radix Angelicae sinensis-free formula (0: 19DSS group).

(2) And conclusion of the same

1) The prescription of the angelica and peony powder with different proportions of angelica and peony can relieve the maxillofacial pain caused by formalin.

2) The ratio of angelica to peony is 0:19, the effect of relieving the maxillofacial pain caused by formalin is best when angelica is not available.

The Danggui Paeonia powder can be prepared into various dosage forms for treating and relieving various inflammatory pains of oral cavity, jaw and face and various inflammatory pains caused by pathological changes of various organs of a body, and the dosage forms comprise decoction, pills, powder, paste, pellet, medicinal liquor, medicinal granules, oral liquid, capsules, tablets, injection and other common dosage forms.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. The application of the angelica sinensis and peony powder in preparing the medicine for treating oromaxillofacial inflammatory pain is characterized in that the angelica sinensis and peony powder is composed of the following medicinal materials in parts by weight: 0 part of Chinese angelica: 19 parts of Chinese herbaceous peony: 4 parts of poria cocos: 4 parts of bighead atractylodes rhizome: 8 parts of rhizoma alismatis: and 8 parts of ligusticum wallichii.

2. The use of Danggui Paeonia powder in the preparation of the medicine for treating oromaxillofacial inflammatory pain as claimed in claim 1, wherein the preparation method of Danggui Paeonia powder comprises the following steps:

s1: selecting high-quality radix Paeoniae, Poria, Atractylodis rhizoma, Alismatis rhizoma and rhizoma Ligustici Chuanxiong according to Chinese medicinal standard;

s2: mixing the peony, the tuckahoe, the atractylodes, the rhizoma alismatis and the ligusticum wallichii mentioned in the S1 according to a proportion to obtain a mixture A;

3. The use of the angelica sinensis peony powder as claimed in claim 2, for preparing a medicament for treating oromaxillofacial inflammatory pain, wherein in S3, the ratio of the volume of the distilled water added to the volume of the mixture a is 8: 1.

4. the use of the angelica sinensis peony powder as claimed in claim 2, wherein the soaking time in S3 is 1h, and the decocting time is 2 h.

5. The use of the angelica sinensis peony powder as claimed in claim 2, in the preparation of a medicament for treating oromaxillofacial inflammatory pain, wherein in S4, the volume ratio of the added distilled water to the first residue B is 6: 1.

6. the use of the angelica sinensis peony powder as claimed in claim 2, for preparing a medicament for treating oromaxillofacial inflammatory pain, wherein in S4, the decoction time is 1.5 h.

7. The use of the angelica sinensis peony powder as claimed in claim 1, for preparing a medicament for treating oral and maxillofacial inflammatory pain, wherein the angelica sinensis peony powder is prepared into various dosage forms for treating and relieving various inflammatory pain symptoms of oral, maxillofacial area, such dosage forms including decoction, pill, powder, ointment, pellet, wine, granule, oral liquid, capsule, tablet and injection.