CN109480295A - Sedge water-soluble dietary fiber and preparation method thereof and defaecation purposes - Google Patents
Sedge water-soluble dietary fiber and preparation method thereof and defaecation purposes Download PDFInfo
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- 241001290610 Abildgaardia Species 0.000 title claims abstract description 116
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000000284 extract Substances 0.000 claims abstract description 75
- 239000000843 powder Substances 0.000 claims abstract description 38
- 239000000835 fiber Substances 0.000 claims abstract description 37
- 239000006228 supernatant Substances 0.000 claims abstract description 28
- 235000005911 diet Nutrition 0.000 claims abstract description 27
- 230000000378 dietary effect Effects 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000004365 Protease Substances 0.000 claims abstract description 19
- 239000004382 Amylase Substances 0.000 claims abstract description 18
- 108010065511 Amylases Proteins 0.000 claims abstract description 18
- 102000013142 Amylases Human genes 0.000 claims abstract description 18
- 108091005804 Peptidases Proteins 0.000 claims abstract description 18
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 18
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- 238000005238 degreasing Methods 0.000 claims abstract description 13
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims abstract description 12
- 239000000341 volatile oil Substances 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 9
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 9
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- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 claims description 6
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- DEXFNLNNUZKHNO-UHFFFAOYSA-N 6-[3-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperidin-1-yl]-3-oxopropyl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1CCN(CC1)C(CCC1=CC2=C(NC(O2)=O)C=C1)=O DEXFNLNNUZKHNO-UHFFFAOYSA-N 0.000 description 5
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- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 2
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
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- 241000193830 Bacillus <bacterium> Species 0.000 description 1
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000045459 Carex meyeriana Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 206010011953 Decreased activity Diseases 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of preparation method of sedge water-soluble dietary fiber extract, and resulting sedge dietary crude fiber powder after extraction sedge essential oil is carried out enzymatic hydrolysis and degreasing, obtains sedge extract；The step of enzymatic hydrolysis are as follows: according to the solid-liquid ratio of 1g:5~15mL, deionized water is added in sedge crude fibre powder, obtains mixed liquor；Plant hydrolyzed-enzyme is successively added in gained mixed liquor, that is, amylase, carbohydrase and protease are hydrolyzed, and merge centrifugation gained supernatant after hydrolyzing three times, obtain extracting solution.The present invention also provides one kind according to the resulting sedge water-soluble dietary fiber extract of above-mentioned preparation method, and retention ability is up to 753%, expansive force 3.65mL/g.The present invention also proposes a kind of defaecation purposes of sedge water-soluble dietary fiber extract, and sedge water-soluble dietary fiber extract and Alfalfa powder are mixed and obtain powderous preparations, has preferable aperient effects.
Description
Technical field
The present invention relates to technical field of natural product extraction, and in particular to a kind of sedge water-soluble dietary fiber and its mentions
Method is taken, and is matched using sedge water-soluble dietary fiber extract preparation relax bowel and defecation food or special medicine purposes
The purposes of square food.
Background technique
Sedge (scientific name: Carexmeyeriana), You Cheng Wu La grass, is Cyperaceae sedge platymiscium, is mainly grown on
On the south Northeast China Changbai Mountain arteries and veins and Wai Xin An Lin (including Karafuto).There is sedge taste removal to dispel taste, clearing and activating the channels and collaterals, elimination
Fatigue, the effect for improving blood microcirculation, improving immunity.
Only sedge is applied in daily field now, usually as production straw sandals, paillasse, synthetic cotton, fiberboard,
The materials such as straw weavings, papermaking, or sedge is further processed, extraction obtains sedge essential oil, for pressing down in vitro
Bacterium does not carry out sufficient processing and utilization to sedge.
Sedge is not inquired temporarily in the application in edible field, does not inquire temporarily yet and extracts dietary fiber from sedge
Pertinent literature and sedge are used for the pertinent literature of relax bowel and defecation.
To sum up, it needs to be further improved the prior art.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of sedge water-soluble dietary fiber and its extracting methods, and
Relax bowel and defecation food or special medicine purposes formula food are prepared using the water solubility sedge dietary fiber, is sedge
Using new direction is provided, frontier is started.
In order to solve the above technical problem, the present invention provides a kind of systems for making sedge water-soluble dietary fiber extract
Preparation Method:
Resulting sedge dietary crude fiber powder after extraction sedge essential oil is subjected to enzymatic hydrolysis and degreasing, obtains sedge
Water-soluble dietary fiber extract；
The step of enzymatic hydrolysis and degreasing, is as follows:
1.1) it, digests:
According to the solid-liquid ratio of 1g:5~15mL, deionized water is added into sedge dietary crude fiber powder, is mixed
Liquid (pH=7)；
Amylase is added into gained mixed liquor, is hydrolyzed 1 ± 0.1 hour at 40~60 DEG C, residue I is obtained after centrifuge separation
And collect supernatant I；
Citric acid-sodium citrate buffer solution (0.1mol/L, pH4.5) is added in residue obtained I, obtains residue mixed liquor
I (pH=4.5)；
Note: citric acid-sodium citrate buffer solution (0.1mol/L, pH4.5) is the prior art, and so there is no need to inform that it is specifically matched
Set mode.
Carbohydrase is added into residue obtained mixed liquor I, is hydrolyzed 1 ± 0.1 hour at 40~60 DEG C, after centrifuge separation
Residue II simultaneously collects supernatant II；
To distilled water is added in residue obtained II, configuration obtains residue mixed liquor II (pH=7)；
Protease is added into residue obtained mixed liquor I, is hydrolyzed 1 ± 0.1 hour at 40~60 DEG C, after centrifuge separation
Waste simultaneously collects supernatant III；
Merge supernatant I, supernatant II and supernatant III, obtain extracting solution.
The deionized water: citric acid-sodium citrate buffer solution: the volume ratio of distilled water be 1:0.8~1.2:0.8~
1.2；
Note: the parameter of above-mentioned centrifugation is to be centrifuged 6 ± 2min in 3000 ± 4000rpm.
1.2) degreasing:
3000 ± 4000rpm of extracting solution obtained by step 1.1) is centrifuged 15 ± 2min, obtains supernatant IV；
IV rotary evaporation of supernatant is concentrated into the 45~55% of stoste volume in 70~100 DEG C of water-baths, obtains concentrate；
1 ± 0.1 times of volume dehydrated alcohol is added in gained concentrate, 3 ± 1h of degreasing under room temperature (25-32 DEG C), later
It is centrifuged 10 ± 2min in 1500 ± 2000rpm, takes precipitating；
Precipitating (is washed the sediment to no ethyl alcohol to remain) wash with distilled water and is dried afterwards, sedge water solubility meals are obtained
Eat fiber extract.
The improvement of preparation method as sedge water-soluble dietary fiber extract of the present invention:
The additive amount of amylase, carbohydrase and protease is as follows in the step 1.1):
The dosage of the amylase is the 3.5~4.5% of sedge dietary crude fiber powder quality；
The dosage of the carbohydrase is the 1.0~1.5% of sedge dietary crude fiber powder quality；
The dosage of the protease is the 2.5~3.0% of sedge dietary crude fiber powder quality.
The further improvement of preparation method as sedge water-soluble dietary fiber extract of the present invention:
The additive amount of amylase, carbohydrase and protease is as follows in the step 1.1):
The dosage of the amylase is the 4.0% of sedge dietary crude fiber powder quality；
The dosage of the carbohydrase is the 1.3% of sedge dietary crude fiber powder quality；
The dosage of the protease is the 2.8% of sedge dietary crude fiber powder quality.
The improvement of preparation method as sedge water-soluble dietary fiber extract of the present invention:
Note: the preparation method of sedge dietary crude fiber powder can refer to " sedge chemical component and pharmacological action just
Step research " in 4.3.2 section, the present invention can for example follow the steps below:
150 grams of sedge powder (40-60 mesh) is taken, supercritical CO is placed in2In extraction equipment, extracting pressure 16MPa,
Extraction temperature is 38 DEG C, and I pressure of extraction-container is 5MPa, and 35 DEG C of temperature, II pressure of extraction-container is 1.5MPa, 32 DEG C of temperature, is extracted
0.08 gram of sedge essential oil is obtained within 1.5 hours, residue is sedge dietary crude fiber powder.
Daily field is belonged to the application of sedge in the prior art, does not find sedge temporarily in the correlation text in edible field
It offers, main cause is that the essential oil of sedge has pharmacological action to human body, and directly edible there is a certain security risk, i.e., directly right
Sedge powder carries out enzymatic hydrolysis and degreasing, does not remove sedge essential oil, and obtaining sedge water-soluble dietary fiber extract does not have
Meaning.Therefore it first has to utilize supercritical CO before preparing sedge water-soluble dietary fiber extract2Extraction removal sedge
Essential oil.
To solve above-mentioned the technical issues of being proposed, it is resulting that the present invention also proposes prepared by a kind of above-mentioned preparation method of utilization
Sedge water-soluble dietary fiber extract.
To solve above-mentioned the technical issues of being proposed, the present invention also proposes a kind of sedge water-soluble dietary fiber extract
Defaecation purposes:
The sedge water-soluble dietary fiber extract is used to prepare the food or special medicine purposes of relax bowel and defecation
Formula food.
The improvement of defaecation purposes as sedge water-soluble dietary fiber extract of the present invention:
The food or special medicine purposes formula food of the relax bowel and defecation are by 80~70% sedge aqueous soluble dietaries
Fiber extract and 20~30% Alfalfa powder mix；
Above-mentioned % is quality %.
The suggested use and dosage of gained powderous preparations of the invention are as follows: two times a day, a 1.5g, a course for the treatment of was (serious in 3-5 days
Constipation person, daily most 6g).
Technical advantage of the invention is mainly reflected in:
1, the present invention can obtain crow using sedge (that is, the sedge dietary crude fiber powder) processing after extraction essential oil
Careless water-soluble dietary fiber is drawn to start sedge new application to make full use of sedge resource.
2, the present invention will remove after sedge essential oil resulting sedge dietary crude fiber powder sequentially (amylase, sugar
Change enzyme, protease) it is hydrolyzed, by the selection of enzyme and the control of dosage, hydrolysis time, to realize that meyer sedge fiber is mild
Under the conditions of hydrolysis, protect environment, reduce energy consumption, the sedge water-soluble dietary fiber of preparation can be the safe and efficient benefit of people
With reaching the requirement of special medicine purposes formula food active element function.
3, the present invention controls extracting solution concentrating degree, so that it is fine to obtain the higher sedge aqueous soluble dietary of yield
Dimension.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This.
The preparation method of embodiment 1, sedge water-soluble dietary fiber extract, successively follows the steps below:
1), enzymatic hydrolysis and degreasing:
1.1), enzymatic hydrolysis:
Resulting 10 grams of sedge dietary crude fiber powder are weighed after extraction sedge essential oil in conical flask, and in the cone
100mL deionized water (pH=7) is added in shape bottle, obtains mixed liquor；
Plant hydrolyzed-enzyme (i.e. amylase, carbohydrase and protease) is added into gained mixed liquor；
Note: amylase uses alpha-amylase (hay bacillus) in the present embodiment, enzyme activity 4000U/g；
The enzyme activity of carbohydrase is 100,000 U/g；
Protease uses papain, enzyme activity 800U/mg；
Order of addition are as follows:
1., add 0.4g amylase (enzyme activity 4000U/g) in Xiang Shangshu mixed liquor, it is small that 1.0 are hydrolyzed at 45~55 DEG C
When, residue I is obtained after centrifuge separation and collects supernatant I；
The dosage of amylase is the 4.0% of sedge dietary crude fiber powder quality in the present embodiment.
2., into step 1. residue obtained I be added citric acid-sodium citrate buffer solution (0.1mol/L, pH4.5) 100mL,
It obtains residue mixed liquor I (pH=4.5)；
0.13g carbohydrase (enzyme activity is 100,000 U/g) is added into residue mixed liquor I, and it is small that 1.0 are hydrolyzed at 45~55 DEG C
When, residue II is obtained after centrifuge separation and collects supernatant II；
The dosage of carbohydrase is the 1.3% of sedge dietary crude fiber powder quality in the present embodiment.
3., into step 2. residue obtained II be added pH=7 distilled water 100mL prepare residue mixed liquor III (pH=7)；
0.28g protease (enzyme activity 800U/mg) is added into residue mixed liquor III, it is small that 1.0 are hydrolyzed at 45~55 DEG C
When, supernatant III, waste are collected in centrifuge separation.
The dosage of protease is the 2.8% of sedge dietary crude fiber powder quality in the present embodiment.
4., merge above-mentioned steps 1.~step 3. 3 times hydrolysis after supernatant, that is, merge supernatant I, II and of supernatant
Supernatant III, obtains extracting solution.
Note: the specific steps being centrifuged after above-mentioned hydrolysis are as follows: be centrifuged 6 ± 2min, the present embodiment in 3000 ± 4000rpm
In in 3000rpm be centrifuged 6min.
1.2), degreasing:
By step, 4. gained extracting solution through 3000rpm is centrifuged 15min, obtains supernatant IV；
IV rotary evaporation of supernatant is concentrated into the 50% of stoste volume in 80~90 DEG C of water-baths, obtains concentrate.
1 times of volume dehydrated alcohol is added in gained concentrate, degreasing 3h under room temperature (27 DEG C), later in 1500rpm from
Heart 10min takes precipitating；
It is precipitated to no ethyl alcohol residual with distillation washing, sets and is deposited in 45~55 DEG C of vacuum ovens dryings to constant weight, obtain
1.0g sedge water-soluble dietary fiber extract, yield 10%.
Comparative example 1-1: hydrolysis time in step 2.1) is changed to " 30min " by " 1.0h ", that is, amylase is in mixed liquor
Middle hydrolysis 30min, carbohydrase hydrolyze 30min in residue mixed liquor I, protease hydrolyzes 30min in residue mixed liquor II；Its
Yu Jun is equal to embodiment 1.
This comparative example obtains 0.65g sedge water-soluble dietary fiber extract, yield 6.5%.
When proving that hydrolysis time is 30min, it cannot effectively hydrolyze sedge crude fibre and be hydrolyzed, influence sedge water
The yield of soluble dietary fiber extract.
Comparative example 1-2, the dosage for increasing plant hydrolyzed-enzyme in step 2.1), that is, the dosage of amylase is changed to by 0.4g
0.8g, carbohydrase dosage 2.6g is changed to by 1.3g, the dosage of protease is changed to 5.6g by 2.8g；Remaining is equal to reality
Apply example 1.
This comparative example obtains 0.72g sedge water-soluble dietary fiber extract, yield 7.2%.
The dosage of each plant hydrolyzed-enzyme doubles in this comparative example, and obtained sedge water-soluble dietary fiber extract is received
Rate is only 7.2%, and since each plant hydrolyzed enzyme dosage increases, cost is improved, uneconomical for producing.
Comparative example 1-3, the concentration amount that supernatant rotary evaporation in step 2.2) is concentrated are changed by " 50% of original volume "
For " the 30% of original volume ", remaining is equal to embodiment 1.
This comparative example obtains 0.55g sedge water-soluble dietary fiber extract, yield 5.5%.
Concentration amount is changed to " the 30% of original volume " by " the 50% of original volume ", sedge water-soluble dietary fiber when processing
The energy that extract loss is increased, and consumed increases, and cost improves.
Comparative example 1-4, the concentration amount that supernatant rotary evaporation in step 2.2) is concentrated are changed by " 50% of original volume "
For " the 70% of original volume ", remaining is equal to embodiment 1.
This comparative example obtains 0.63g sedge water-soluble dietary fiber extract, yield 6.3%.
Concentration amount is changed to " the 70% of original volume " by " the 50% of original volume ", sedge water-soluble dietary fiber when processing
Extract loss increases, and subsequent process dehydrated alcohol dosage increases, and processing cost improves.
Test 1, analysis of physical and chemical property:
(1) measurement of retention ability:
1 gained sedge water-soluble dietary fiber 100mg of embodiment is weighed to be drained, weighed with 20 DEG C of distillation water saturation 1h
And it calculates.
Retention ability are as follows: Pw=(mw-md)/md × 100%
Wherein: mw is the quality of wet sample, unit g；
Md is the quality of dry-eye disease, unit g.
The results are shown in Table 1:
Table 1
| Group | md(g) | mw(g) | Pw (%) |
| Embodiment 1 | 0.100 | 0.853 | 753 |
(2) expansive force measures:
Sedge dietary fiber 100mg is weighed, is placed in 10mL graduated cylinder, its volume is recorded；5mL distilled water, oscillation is added
It is put into 20 DEG C of insulating box heat preservations after uniformly for 24 hours, records volume shared after sedge dietary fiber expands again.
Expansive force are as follows: Ps=(V1-V2)/md
In formula: V1For the volume of fiber after swelling, mL；
V2For the volume of dry-eye disease, mL；
Md is the quality of dry-eye disease, unit g, md 0.1g.The results are shown in Table 2:
Table 2
| Group | V2(mL) | V1(mL) | Ps(mL/g) |
| Embodiment 1 | 5.00 | 5.365 | 3.65 |
Above-mentioned all comparative examples carry out analysis of physical and chemical property according to experiment 1, and the results are shown in Table 3 for concrete analysis:
Table 3
| Group | Pw (%) | Ps(mL/g) |
| Embodiment 1 | 753 | 3.65 |
| Comparative example 1-1 | 705 | 2.88 |
| Comparative example 1-2 | 701 | 2.66 |
| Comparative example 1-3 | 702 | 2.76 |
| Comparative example 1-4 | 703 | 2.88 |
The defaecation purposes of embodiment 2, sedge water-soluble dietary fiber extract；
Relax bowel and defecation food or special medicine purposes are prepared using 1 sedge water-soluble dietary fiber extract of embodiment
Formula food (drug as treated constipation), collectively referred to hereinafter as extract formula food；
Extract formula food is made of the sedge water-soluble dietary fiber and Alfalfa powder obtained with the present invention, and
The weight of sedge water-soluble dietary fiber extract is 80~70%, Alfalfa powder weight is 20~30%, obtains powdery
Preparation.
In the present embodiment, 2.4g sedge water-soluble dietary fiber extract (preparation of embodiment 1 gained) and 0.6g lucerne are taken
Mu grass meal end, after being mixed uniformly, obtains powderous preparations, i.e. relax bowel and defecation powdered food product.
Test 2, foodsafety animal experiment:
It is completed in Zhejiang University's animal experimental center.
SPF grades of ICR male mices are purchased from Zhejiang Academy of Medical Sciences Experimental Animal Center, quality certification number SCXK (Zhejiang)
Mouse is randomly divided into 8 groups before experiment by 20170033,20 ± 2g of weight, half male and half female, 4-6 week old, and every group 10, respectively
Blank control group (physiological saline with tested sample same amount), the positive controls (wheat of commercially available Sweden RecipAB company
Cellulose grain (Fiberform Fiberform)) and the prepared powderous preparations that obtain of the embodiment of the present invention 2 (that is, relax bowel and defecation powder
End) normal dose extract group, 5 multiple dose extract groups, 10 multiple dose extract groups, 15 multiple dose extract groups, 20 times
Dosage extract group I (dosage 25mL/kg), 20 multiple dose extract groups II (dosage 37.25mL/kg), specific data such as 4 institute of table
Show.
12h is deprived of food but not water before testing, and stomach-filling volume is as shown in table 4, and maximum dose level is that 20 multiple dose extracts are filled
Stomach carries out acute toxicity test.After each group stomach-filling is to food, according to conventinal breeding, gives after food within 2 hours, observed every 15 minutes
Once；It is primary every observation in 30 minutes within after food 2-4 hours；It is primary every observation in 60 minutes within after food 4-8 hours, it is main
Observe mouse feed drinking-water situation, external reaction, adnormal respiration, skin color change etc. toxic reactions and death condition.
It is continuous to give food 7 days, and observed, test result is shown in Table 4:
Table 4
| Concentration | Dosage (mL/kg) | Quantity (only) | The dead quantity (only) |
| Blank group | 25 | 10 | 0 |
| Positive controls | 25 | 10 | 0 |
| Normal dose extract group | 25 | 10 | 0 |
| 5 multiple dose extract groups | 25 | 10 | 0 |
| 10 multiple dose extract groups | 25 | 10 | 0 |
| 15 multiple dose extract groups | 25 | 10 | 0 |
| 20 multiple dose extract groups I | 25 | 10 | 0 |
| 20 multiple dose extract groups II | 37.25 | 10 | 0 |
The result shows that mouse growth is all right to during food, breathing, feed, activity and its stool and urine have no different
Often.Animal is put to death after a week and is dissected, and by the naked eye, the main organs such as the heart, liver, lung appearance does not have obvious lesion phenomenon.
It is safe that the above results, which show that the present invention extracts composition formula,.
Experiment 3, the animal simulation constipation of extract formula food test
Influence of the extract formula food to Constipation Model mouse defecation time and defecation grain number: mouse 60 are taken, male and female
Fifty-fifty, be randomly divided into 6 groups, every group 10: blank control group, model control group, positive group be (commercially available Sweden RecipAB company
Semen Tritici aestivi fiber crude granule (Fiberform Fiberform), 2 gained powderous preparations of embodiment (that is, extract formula food) it is normal
Dosage extract experimental group, 10 multiple dose extract experimental groups, 20 multiple dose extract experimental groups.Blank control group is routinely raised
It supports；
In addition to blank control group, other each groups obtain Constipated mice as follows:
Every group of mouse gives sufficient drinking-water, ad lib daily.Light-coloured vinegar 0.3mL is given in stomach-filling in 1st day, from next day by
Light-coloured vinegar is given in 10mL/kg stomach-filling, and continuous 9 days；Stomach-filling gave light-coloured vinegar while dividing 2 stomach-fillings to give by 40mL/kg in the 7th day
10% 0 DEG C of active carbon ice water, for three days on end, each stomach-filling interval time is 4h.Mice with constipation model successfully indicates are as follows: small
Mouse whole body underactivity, hair is loose, matt, and times of defecation is reduced, and excrement is dry and hard, and faecal pellet is tiny etc..It is each after modeling success
For 24 hours, blank control group and model control group press the stomach-filling water supply of 25mL/kg volume for group fasting；Positive drug group gives commercially available Sweden
Semen Tritici aestivi fiber crude granule (Fiberform Fiberform) 0.27g/kg amount stomach-filling of RecipAB company；This extract formula food is just
Normal dosage extract experimental group, 10 multiple dose extract experimental groups, the food volume of giving of 20 multiple dose extract experimental groups are 25mL/
Kg weight.It is respectively placed in the beaker for be covered with filter paper and is observed to each group after food, after 1h, the first pellet is discharged just in start recording
Time, while record 4h to food in defecation total grain number.Statistical data, and carry out statistical analysis.As can be known from Table 5: mould
Type control group has extremely significant difference (#P < 0.01) compared with blank control group, illustrates modeling success.
Table 5
As seen from the above table, each administration group can be obviously shortened defecation time compared with model control group, increase defecation grain number
(* P < 0.01), there were significant differences between 20 multiple dose extract experimental groups and positive drug group (Biantong capsule) property (* P <
0.05).Illustrating this extract formula food and Biantong capsule all has the function of promoting mice with constipation defecation, wherein being extracted with original
The effect of 20 multiple dose extract experimental group of composition formula food is most significant.
The dosage of meyer sedge fiber water-soluble in embodiment 2 is changed to 2.1g, Alfalfa powder by 2.4g by embodiment 3
Dosage 0.9g is changed to by 0.6g, remaining is equal to embodiment 2.
Comparative example 2-1, cancel Alfalfa powder in embodiment 2, and by the weight of sedge water-soluble dietary fiber extract
Amount is changed to 3.0g, that is, total weight remains unchanged, remaining is equal to embodiment 2.
Comparative example 2-2, the dosage of meyer sedge fiber water-soluble in embodiment 2 is changed to 1.8g, alfalfa meal by 2.4g
The dosage at end is changed to 1.2g by 0.6g, remaining is equal to embodiment 2.
Comparative example 2-3, the dosage of meyer sedge fiber water-soluble in embodiment 2 is changed to 1.2g, alfalfa meal by 2.4g
The dosage at end is changed to 1.8g by 0.6g, remaining is equal to embodiment 2.
Comparative example 2-4, the dosage of meyer sedge fiber water-soluble in embodiment 2 is changed to 0.6g, alfalfa meal by 2.4g
The dosage at end is changed to 2.4g by 0.6g, remaining is equal to embodiment 2.
Comparative example 2-5, cancel sedge water-soluble dietary fiber extract in embodiment 2, and by the weight of Alfalfa powder
Amount is changed to 3.0g, that is, total weight remains unchanged, remaining is equal to embodiment 2.
Note: powderous preparations obtained by this comparative example 2-5 all use Alfalfa powder that patient can be made to be difficult to swallow.
Above-described embodiment 3 and comparative example 2-1 to comparative example 2-5 are prepared into resulting powderous preparations and carry out reality according to experiment 3
It tests, the influence measured under each powderous preparations normal dose to Constipation Model mouse defecation time is as shown in table 6:
Table 6
| Defecation time (min) | |
| Embodiment 2 | 30.26±2.12 |
| Embodiment 3 | 35.72±2.22 |
| Comparative example 2-1 | 75.01±2.89 |
| Comparative example 2-2 | 40.65±2.32 |
| Comparative example 2-3 | 55.83±2.65 |
| Comparative example 2-4 | 65.05±2.77 |
| Comparative example 2-5 | 79.12±3.33 |
As shown in Table 6, embodiment 2 and embodiment 3 prepare resulting powderous preparations and all have preferable aperient effects, and right
It is poor that ratio comparative example 2-1 to comparative example 2-5 prepares resulting powderous preparations aperient effects.
The above list is only a few specific embodiments of the present invention for finally, it should also be noted that.Obviously, this hair
Bright to be not limited to above embodiments, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (6)
1. the preparation method of sedge water-soluble dietary fiber extract, it is characterized in that:
Resulting sedge dietary crude fiber powder after extraction sedge essential oil is subjected to enzymatic hydrolysis and degreasing, it is water-soluble to obtain sedge
Property dietary fiber extract；
The step of enzymatic hydrolysis and degreasing, is as follows:
1.1) it, digests:
According to the solid-liquid ratio of 1g:5~15mL, deionized water is added into sedge dietary crude fiber powder, obtains mixed liquor；
Amylase is added into gained mixed liquor, is hydrolyzed 1 ± 0.1 hour at 40~60 DEG C, residue I is obtained after centrifuge separation and is received
Collect supernatant I；
To citric acid-sodium citrate buffer solution is added in residue obtained I, residue mixed liquor I is obtained；
Carbohydrase is added into residue obtained mixed liquor I, is hydrolyzed 1 ± 0.1 hour at 40~60 DEG C, residue is obtained after centrifuge separation
II and collect supernatant II；
To distilled water is added in residue obtained II, residue mixed liquor II is obtained；
Protease is added into residue obtained mixed liquor II, is hydrolyzed 1 ± 0.1 hour at 40~60 DEG C, waste after centrifuge separation
And collect supernatant III；
Merge supernatant I, supernatant II and supernatant III, obtains extracting solution；
The deionized water: citric acid-sodium citrate buffer solution: the volume ratio of distilled water is 1:0.8~1.2:0.8~1.2；
1.2) degreasing:
3000 ± 4000rpm of extracting solution obtained by step 1.1) is centrifuged 15 ± 2min, obtains supernatant IV；
IV rotary evaporation of supernatant is concentrated into the 45~55% of stoste volume in 70~100 DEG C of water-baths, obtains concentrate；
Be added 1 ± 0.1 times of volume dehydrated alcohol in gained concentrate, 3 ± 1h of degreasing at room temperature, later in 1500 ±
2000rpm is centrifuged 10 ± 2min, takes precipitating；
Precipitating is dried afterwards wash with distilled water, obtains sedge water-soluble dietary fiber extract.
2. the preparation method of sedge water-soluble dietary fiber extract according to claim 1, it is characterized in that:
The additive amount of amylase, carbohydrase and protease is as follows in the step 1.1):
The dosage of the amylase is the 3.5~4.5% of sedge dietary crude fiber powder quality；
The dosage of the carbohydrase is the 1.0~1.5% of sedge dietary crude fiber powder quality；
The dosage of the protease is the 2.5~3.0% of sedge dietary crude fiber powder quality.
3. the preparation method of sedge water-soluble dietary fiber extract according to claim 2, it is characterized in that:
The additive amount of amylase, carbohydrase and protease is as follows in the step 1.1):
The dosage of the amylase is the 4.0% of sedge dietary crude fiber powder quality；
The dosage of the carbohydrase is the 1.3% of sedge dietary crude fiber powder quality；
The dosage of the protease is the 2.8% of sedge dietary crude fiber powder quality.
4. preparing resulting sedge water-soluble dietary fiber extract using any preparation method of claims 1 to 33.
5. the defaecation purposes of sedge water-soluble dietary fiber extract as claimed in claim 4, it is characterised in that:
The sedge water-soluble dietary fiber extract is used to prepare the food or special medicine purposes formula of relax bowel and defecation
Food.
6. the defaecation purposes of sedge water-soluble dietary fiber extract according to claim 5, it is characterized in that:
The food or special medicine purposes formula food of the relax bowel and defecation are by 80~70% sedge water-soluble dietary fibers
Extract and 20~30% Alfalfa powder mix；
Above-mentioned % is quality %.
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