CN109480126A - Litopenaeus vannamei composite feed additive - Google Patents

Litopenaeus vannamei composite feed additive Download PDF

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Publication number
CN109480126A
CN109480126A CN201811517168.4A CN201811517168A CN109480126A CN 109480126 A CN109480126 A CN 109480126A CN 201811517168 A CN201811517168 A CN 201811517168A CN 109480126 A CN109480126 A CN 109480126A
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China
Prior art keywords
litopenaeus vannamei
follows
feed additive
percentage composition
raw material
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CN201811517168.4A
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Chinese (zh)
Inventor
许青松
薛壮
衣启麟
刘超
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Dalian Ocean University
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Dalian Ocean University
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Priority to CN201811517168.4A priority Critical patent/CN109480126A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Insects & Arthropods (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a kind of litopenaeus vannamei composite feed additive, raw material percentage compositions are as follows: monosaccharide preparation 20 ~ 60%, chlorella powder 5 ~ 10%, ocean rhodotorula glutinis powder 5 ~ 10%, vitamin E 1 ~ 5%, surplus are the maltodextrins as carrier;The monosaccharide preparation raw material percentage composition is as follows: fucose 5 ~ 10%, N- acetylgalactosamine 5 ~ 10%, N-acetylglucosamine 5 ~ 10%, glucuronic acid 5 ~ 10%, sialic acid 5 ~ 10%, galactolipin 10 ~ 20%, Glucosamine 10 ~ 20%, mannose 10 ~ 20% and xylose 10 ~ 20%.Each component is mutually coordinated to play a role, litopenaeus vannamei body's immunity can be effectively improved, the growth and shell rate of litopenaeus vannamei are promoted, improves efficiency of feed utilization, reduce aquaculture cost, be it is a kind of have no toxic side effect, the green safe feed addictive of drug residue free.

Description

Litopenaeus vannamei composite feed additive
Technical field
The invention belongs to natural products development technique field and aquatic feeds technical field more particularly to a kind of vannamei boones pair Shrimp composite feed additive.
Background technique
Litopenaeus vannamei, also known as Penaeus Vannmei have to water environment high-output stress-resistance, nutritional requirement is low, the speed of growth Fastly, the big equal many merits of shrimp body meat content, are currently one of big shrimp species of China's cultured output highest three.Its is intensive in recent years Cultivation scale increases year by year, and disease problem is got worse, and a large amount of antibiotic are used for controlling disease, not only reduce vannamei boone The nutritive value of prawn, or even endanger the health of the mankind.
Summary of the invention
The present invention is to provide a kind of litopenaeus vannamei with compound to solve above-mentioned technical problem present in the prior art Feed addictive.
The technical solution of the invention is as follows: a kind of litopenaeus vannamei composite feed additive, raw material percentage composition Are as follows: monosaccharide preparation 20 ~ 60%, chlorella powder 5 ~ 10%, ocean rhodotorula glutinis powder 5 ~ 10%, vitamin E 1 ~ 5%, surplus are as carrier Maltodextrin;The monosaccharide preparation raw material percentage composition is as follows: fucose 5 ~ 10%, N- acetylgalactosamine 5 ~ 10%, N- Acetylglucosamine 5 ~ 10%, glucuronic acid 5 ~ 10%, sialic acid 5 ~ 10%, galactolipin 10 ~ 20%, Glucosamine 10 ~ 20%, mannose 10 ~ 20% and xylose 10 ~ 20%.
Preferred raw material percentage composition example are as follows: monosaccharide preparation 50%, chlorella powder 6%, ocean rhodotorula glutinis powder 6%, vitamin E 3%, Maltodextrin 35%.
It is preferred that monosaccharide preparation raw material percentage composition is as follows: fucose 6%, N- acetylgalactosamine 6%, N- acetylamino Portugal Grape sugar 8%, glucuronic acid 8%, sialic acid 8%, galactolipin 16%, Glucosamine 16%, mannose 16% and xylose 16%.
It is preferred that monosaccharide preparation raw material percentage composition is as follows: fucose 5%, N- acetylgalactosamine 5%, N- acetylamino Portugal Grape sugar 7%, glucuronic acid 7%, sialic acid 8%, galactolipin 17%, Glucosamine 17%, mannose 17% and xylose 17%.
It is preferred that monosaccharide preparation raw material percentage composition is as follows: fucose 5%, N- acetylgalactosamine 5%, N- acetylamino Portugal Grape sugar 5%, glucuronic acid 5%, sialic acid 10%, galactolipin 15%, Glucosamine 15%, mannose 20% and xylose 20%.
The present invention is with fucose, N- acetylgalactosamine, N-acetylglucosamine, glucuronic acid, sialic acid, half Lactose, Glucosamine, mannose and xylose form monosaccharide preparation, then give birth to chlorella powder, ocean rhodotorula glutinis powder 5 ~ 10% and dimension Plain E rational proportion, each component is mutually coordinated to play a role, and can effectively improve litopenaeus vannamei body's immunity, promotes all receive The growth and shell rate of shore prawn, improve efficiency of feed utilization, reduce aquaculture cost, be one kind have no toxic side effect, drug residue free Green safe feed addictive.
Specific embodiment
Embodiment 1:
Litopenaeus vannamei composite feed additive of the invention, raw material percentage composition are as follows: monosaccharide preparation 50%, chlorella powder 6%, ocean rhodotorula glutinis powder 6%, vitamin E 3%, maltodextrin 35%, wherein monosaccharide preparation raw material percentage composition is as follows: rock algae Sugared 6%, N- acetylgalactosamine 6%, N-acetylglucosamine 8%, glucuronic acid 8%, sialic acid 8%, galactolipin 16%, amino Glucose 16%, mannose 16% and xylose 16%.
Preparation method is that 9 kinds of monosaccharide for first being included by monosaccharide preparation are uniformly mixed, then with chlorella powder, ocean rhodotorula Powder, vitamin E, maltodextrin etc. are uniformly mixed according to composition mass percent, and raw materials used is outsourcing.According to matter when use Amount percentage is added in the basal feed of litopenaeus vannamei for 0.001 ~ 1%.
Embodiment 2:
Litopenaeus vannamei composite feed additive of the invention, raw material percentage composition are as follows: monosaccharide preparation 50%, chlorella powder 6%, ocean rhodotorula glutinis powder 6%, vitamin E 3%, maltodextrin 35%, wherein monosaccharide preparation raw material percentage composition is as follows: rock algae Sugared 5%, N- acetylgalactosamine 5%, N-acetylglucosamine 7%, glucuronic acid 7%, sialic acid 8%, galactolipin 17%, amino Glucose 17%, mannose 17% and xylose 17%.
Preparation method and application method are the same as embodiment 1.
Embodiment 3:
Litopenaeus vannamei composite feed additive of the invention, raw material percentage composition are as follows: monosaccharide preparation 50%, chlorella powder 6%, ocean rhodotorula glutinis powder 6%, vitamin E 3%, maltodextrin 35%, wherein monosaccharide preparation raw material percentage composition is as follows: rock algae Sugared 5%, N- acetylgalactosamine 5%, N-acetylglucosamine 5%, glucuronic acid 5%, sialic acid 10%, galactolipin 15%, ammonia Base glucose 15%, mannose 20% and xylose 20%.
Preparation method and application method are the same as embodiment 1.
In order to further explain effect of the invention, following test is provided:
1. experimental animal and grouping
Culture experiment is cultivated in the feed factory canopy of Tianjin and is carried out in water system.Bouncing litopenaeus vannamei is selected to carry out temporary It supports, during which feeds basal feed, 2 times a day.After temporarily supporting, select original body mass all for the bouncing of 0.12 ± 0.00g It receives 360 tail of shore prawn, is randomly divided into 3 groups (control group, test one group, two groups of embodiments), every group of 3 repetitions, each repetition 40 Tail shrimp, be satiated with food in 8:30,12:30 and 17:30 respectively daily feeds the feed (continuously feeding 30 min) of each group, control group Basal feed is only fed, one group of test feeds answering for the preparation of embodiment 3 for adding that mass percent is 0.1% in basal feed Feed addictive is closed, two groups of test feeds the embodiment 3 that addition mass percent is 0.1% in basal feed and prepares compound feeding Monosaccharide preparation used in feed additives.The health status of observation litopenaeus vannamei and ingest situation daily, record food ration and Shell number.Suck residual bait every morning, each quantity of exchanged water is cabinet 30%.Natural lighting during feeding experiment, salinity are 2 ‰- 3 ‰, water temperature is 26 ± 3 DEG C, dissolved oxygen amount >=6mg/L, pH 7.8 ~ 8.2, and breeding cycle is 56 d.
2. test specimen acquisition and calculation formula
At the end of feeding experiment, stop eating 24 h, fish for each duplicate shrimp counted, each repetition of weighing, select 10 at random Tail prawn separates hepatopancrease, for the measurement of Antioxidant Indexes etc. to be immunized.
Rate of body weight gain=100 × (Wt-W0)/W0
Specific growth rate=100 × (lnWt-lnW0)/t
Feed coefficient=F/(Wt-W0)
Survival rate=100 × Nf/ Ni
Food ration=F/ [(Ni+Nf)/2]
In formula: W0Shrimp weight (g) when for on-test;WtShrimp weight (g) when for off-test;F is Feed consumption (g);T is It tests number of days (d);NfFor terminal mantissa;NiFor initial mantissa.
3. test result and analysis
By 8 weeks feeding experiments, control group and different tests group were to the rate of body weight gain of litopenaeus vannamei, specific growth rate, average Feed intake, survival rate, shell rate and feed coefficient have an impact as shown in table 1.One group is tested, two groups of rates of body weight gain is tested, is specific The indexs such as growth rate, feed coefficient and shell rate are better than control group, wherein it is high to test rate of body weight gain and specific growth rate in one group In other groups, feed coefficient reaches minimum, and shell rate reaches highest.
Influence of 1 composite feed additive of table to Growth of Litopenaeus vannamei performance and feed conversion
By 8 weeks feeding experiments, control group and different tests group were to the superoxide dismutase (SOD) of litopenaeus vannamei, third Dialdehyde (MDA), glutathione peroxidase (GSH-PX), total antioxidation enzyme (T-AOC), lysozyme (LZM) etc. have an impact, It is shown in Table 2.Test one group, test two groups of SOD, MDA, GSH-PX, T-AOC, LZM be better than control group, wherein testing in one group SOD, T-AOC and LZM enzyme activity highest, MDA vigor are minimum;Although the function and effect for testing two groups are better than control group, each anti- Oxidase active index is lower than one group of test.
Influence of 2 composite feed additive of table to litopenaeus vannamei liver pancreas activities of antioxidant enzymes
In summary: after adding composite feed additive of the present invention in litopenaeus vannamei basal feed, vannamei boone pair can be promoted The growth and shell rate of shrimp improve body's immunity (premunition of enhancing prawn), effectively improve efficiency of feed utilization, reduce Aquaculture cost.

Claims (5)

1. a kind of litopenaeus vannamei composite feed additive, it is characterised in that raw material percentage composition are as follows: monosaccharide preparation 20 ~ 60%, chlorella powder 5 ~ 10%, ocean rhodotorula glutinis powder 5 ~ 10%, vitamin E 1 ~ 5%, surplus is the maltodextrin as carrier;Institute It is as follows to state monosaccharide preparation raw material percentage composition: fucose 5 ~ 10%, N- acetylgalactosamine 5 ~ 10%, N-acetylglucosamine 5 ~ 10%, glucuronic acid 5 ~ 10%, sialic acid 5 ~ 10%, galactolipin 10 ~ 20%, Glucosamine 10 ~ 20%, mannose 10 ~ 20% With xylose 10 ~ 20%.
2. litopenaeus vannamei composite feed additive according to claim 1, it is characterised in that raw material percentage composition example Are as follows: monosaccharide preparation 50%, chlorella powder 6%, ocean rhodotorula glutinis powder 6%, vitamin E 3%, maltodextrin 35%.
3. litopenaeus vannamei composite feed additive according to claim 1 or claim 2, it is characterised in that the monosaccharide preparation is former Expect that percentage composition is as follows: fucose 6%, N- acetylgalactosamine 6%, N-acetylglucosamine 8%, glucuronic acid 8%, saliva Liquid acid 8%, galactolipin 16%, Glucosamine 16%, mannose 16% and xylose 16%.
4. litopenaeus vannamei composite feed additive according to claim 1 or claim 2, it is characterised in that the monosaccharide preparation is former Expect that percentage composition is as follows: fucose 5%, N- acetylgalactosamine 5%, N-acetylglucosamine 7%, glucuronic acid 7%, saliva Liquid acid 8%, galactolipin 17%, Glucosamine 17%, mannose 17% and xylose 17%.
5. litopenaeus vannamei composite feed additive according to claim 1 or claim 2, it is characterised in that the monosaccharide preparation is former Expect that percentage composition is as follows: fucose 5%, N- acetylgalactosamine 5%, N-acetylglucosamine 5%, glucuronic acid 5%, saliva Liquid acid 10%, galactolipin 15%, Glucosamine 15%, mannose 20% and xylose 20%.
CN201811517168.4A 2018-12-12 2018-12-12 Litopenaeus vannamei composite feed additive Pending CN109480126A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679794A (en) * 2019-11-15 2020-01-14 扬州工业职业技术学院 Disease-preventing and yield-increasing crab feed

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0838064A (en) * 1994-07-26 1996-02-13 Meiji Seika Kaisha Ltd Feed capable of preventing infection of harmful bacterium
JP2010159240A (en) * 2009-01-09 2010-07-22 Takashi Kodera Composition having tissue or cell injury restoration ability and internal cleaning ability for mammal
CN102892303A (en) * 2010-04-14 2013-01-23 营养科学股份有限公司 Feed supplement comprising oligosaccharides and medium chain fatty acids
CN104642735A (en) * 2013-11-15 2015-05-27 中国科学院过程工程研究所 Complex carbohydrate preparation for forage, forage containing complex carbohydrate preparation, and use of forage
CN108125071A (en) * 2018-01-25 2018-06-08 青岛科海生物有限公司 A kind of feed for litopenaeus vannamei additive and its application method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0838064A (en) * 1994-07-26 1996-02-13 Meiji Seika Kaisha Ltd Feed capable of preventing infection of harmful bacterium
JP2010159240A (en) * 2009-01-09 2010-07-22 Takashi Kodera Composition having tissue or cell injury restoration ability and internal cleaning ability for mammal
CN102892303A (en) * 2010-04-14 2013-01-23 营养科学股份有限公司 Feed supplement comprising oligosaccharides and medium chain fatty acids
CN104642735A (en) * 2013-11-15 2015-05-27 中国科学院过程工程研究所 Complex carbohydrate preparation for forage, forage containing complex carbohydrate preparation, and use of forage
CN108125071A (en) * 2018-01-25 2018-06-08 青岛科海生物有限公司 A kind of feed for litopenaeus vannamei additive and its application method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李健,等: "《海水健康养殖与质量控制》", 30 November 2012, 中国海洋大学出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679794A (en) * 2019-11-15 2020-01-14 扬州工业职业技术学院 Disease-preventing and yield-increasing crab feed

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