CN109479958A - A kind of refrigerant Yoghourt and preparation method thereof for controlling abscess of throat - Google Patents

Abstract

The invention belongs to yogurt production technical fields, disclose a kind of cold-type yellow (clear) Yoghourt for treating abscess of throat and preparation method, raw material fresh milk is purified, adjust fat standardising, middle addition Wan Niangan after impurity screening, radix polygonati officinalis, purslane, frangipanis, summer mushroom grass, mulberry leaf, peppermint, watermelon peel, Kiwi berry, pears, filtered juice made of sugarcane, carrying out homogeneous, sterilizing, inoculation processing, for solidification type yoghourt, first dispense post-fermentation, it is cooled down again, after-ripening, refrigeration, Yoghourt is made, for stirred yoghurt, it first ferments and dispenses afterwards, Yoghourt is made after carrying out cooling again.The present invention passes through two kinds of yogurt production modes, make Yoghourt that there is the specific function for the treatment of abscess of throat, people can only can achieve the effect that treat abscess of throat by the Yoghourt drunk in this way, without spending many time to go to buy medicine and decocting medicinal herbs, not have to the bitter taste for enduring drug also.

Description

A kind of refrigerant Yoghourt and preparation method thereof for controlling abscess of throat

Technical field

The invention belongs to yogurt production technical field more particularly to a kind of preparation sides for the refrigerant Yoghourt for controlling abscess of throat Method.

Background technique

Currently, the prior art commonly used in the trade is such that

In autumn and winter, weather is very dry, it is easy to the phenomenon that abscess of throat occurs.Abscess of throat is oropharynx and laryngopharynx portion The cardinal symptom of lesion, characterized by bottleneck throat congestion and swelling pain, swallowing discomfort, also known as " sore throat ".Abscess of throat is that people are most normal There are many one of disease symptoms seen, the reason of causing, such as do not pay attention to and excessively use larynx on flu, tonsillitis, diet Deng now with the drugs and folk prescription of many treatment sphagitis, many people are also selected at family and look for a medicine or buy to pharmacy Point medicine is eaten.It is quick that Western medicine treats any disease all since ancient times, but side effect is big, and dependence can be generated by taking Western medicine for a long time With so that body is generated certain resistance to a certain drug.Chinese medicine is adjusted in focusing on, but it is relatively slow to take effect, and Chinese medicine is typically all to decoct It is more troublesome.Chinese and Western medicine has bitter taste, especially Chinese medicine, and many people are difficult to take, especially child.

Yoghourt is that a certain proportion of sucrose is added using fresh milk as raw material, after high temperature sterilization is cooling, is added A kind of dairy produce made of pure lactic acid bacteria culturers culture, taste is sour-sweet fine and smooth, full of nutrition.Its nutritive value is better than fresh milk With various milk powder.The certain strains of fermentation process make sugar, protein in milk have 20% or so to be hydrolyzed into for small molecule (such as gala Sugar and lactic acid, small peptide chain and amino acid etc.).Fat content is usually 3%-5% in milk.Fatty acid after fermented, in cream Increase by 2 times than raw material milk.These variations keep Yoghourt more easy to digest and absorb, and the utilization rate of various nutrients is improved.Acid Milk is fermented by plain chocolate, and in addition to the whole nutritional ingredients for remaining fresh milk, lactic acid bacteria also be can produce during the fermentation Multivitamin necessary to human nutrition, such as VB1, VB2, VB6, VB12.

Due to the rapid development of science and technology, people are distinctly understood that, the food composition that many is beneficial to health, And various diseases generation and diet correlation, and by improve diet condition and play food itself physiology tune Function is saved, and achievees the purpose that improve human health.The particular matter with unique effects is added in Yoghourt, Yoghourt can be made With unique function, so that functional yoghourt be made.Therefore the substance that can alleviate abscess of throat is added in Yoghourt, makes Yoghourt has the specific function for the treatment of abscess of throat, and such people only can reach treatment pharynx by the Yoghourt drunk The effect of larynx swelling and pain does not have to the bitter taste for enduring drug also without spending many time to go to buy medicine and decocting medicinal herbs.

In conclusion problem of the existing technology is:

Abscess of throat is one of most common disease symptoms of people, whenever sick, need specially to buy medicine and take；Western medicine pair is made With big, long-term use can generate dependence and body is made to generate certain resistance to a certain drug；Chinese medicine takes effect relatively slowly, and in Medicine typically decocts more troublesome；Chinese and Western medicine has bitter taste, especially Chinese medicine, and many people are difficult to take, especially child.

Solve the difficulty and meaning of above-mentioned technical problem:

On the one hand main difficulty of the invention is the combination by milk and Chinese medicine and utilizes the fermentation in production process Process removes dense flavour of a drug, and as addition sucrose and fruit juice (watermelon peel, Kiwi berry, pears and sugar cane crushing) come obtained by making The yoghourt-flavored arrived is pure, clean taste.Further aspect is that effect of the invention, especially vitro detection are examined, the present invention It is swollen that treatment throat is observed by establishing the external inflammatory model of lipopolysaccharides (LPS) stimulating expression of macrophage strain RAW246.7 activation The anti-inflammatory effect of pain Yoghourt.If launch is made in the present invention, a kind of new side for improving abscess of throat state will be brought Formula, the state for allowing people to pass through a kind of method of light pleasure to keep fit.

Summary of the invention

In view of the problems of the existing technology, the present invention provides a kind of functional yoghourts for treating abscess of throat.

The invention is realized in this way a kind of functional yoghourt for treating abscess of throat, solidification type yoghourt preparation method, Include:

Step 1, the processing of ingredient: preparation filtered juice.By Wan Niangan, purslane, frangipanis, peppermint and folium mori homogenate after It filters filter residue and takes juice, watermelon peel, Kiwi berry, pears and sugar cane crushing take juice.Each raw material is that (gross mass is by weight percentage 250g): Wan Niangan 5%~10%, radix polygonati officinalis 5%~8%, frangipanis 3%~5%, summer mushroom grass 3%~5%, purslane 3%~ 5%, (or Kiwi berry, pears, sugarcane, note: this ingredient is to have Yoghourt for peppermint 8%~15%, mulberry leaf 5%~10%, watermelon peel A series of different tastes) 6%~10%, starting milk 50%~65%, sucrose 3%~5%；

Milk bottle disinfection: step 2 milk bottle is used boiling water boiling 15 minutes in stainless-steel pan；

Step 3, the purification of cow's milk: with specially designed centrifuge, the white blood cell removed in cow's milk is visible with other naked eyes Foreign matter；

Normalising fat content: step 4 the skimmed milk of certain volume is added in the high cow's milk of fat content, or passes through Seperator isolates dilute cream from cow's milk, then mixes a certain amount of dilute cream again in obtained skimmed milk；

Step 5, ingredient: being heated to 60 DEG C or so for raw milk, and the sucrose of 3%-5%, sugar dissolution are added in modulation cream Afterwards, then filtering and impurity removing adds the filtered juice of step 1 preparation；

Two methods of heating and the appropriate combination of homogeneous are got up to handle, raw milk are first adjusted to 60 DEG C of left sides by step 6, homogeneous The right side carries out homogenization to cream in 8~10Mpa pressure then in homogenizer；

Step 7, sterilizing: there are two types of the sterilizing methods of the raw milk after homogeneous, and cream is heated to 90 DEG C, keeps the temperature 5min, or It is placed in 80 DEG C of thermostat water baths and sterilizes 15 minutes, or use ultra high temperature short time sterilization method (keeping the temperature 2~3s at 135 DEG C)；

Inoculation: the working stock culture that inoculum concentration is 2~3% is added in step 8 in the raw milk sterilized to 43~45 DEG C. For the Mixed Microbes of streptococcus thermophilus and lactobacillus fermenti, the ratio of two kinds of bacterium is 1:1 or 2:1.The inoculum concentration of coccus is slightly some more, can be more It mends since bacillus grows the shortcomings that producing acid and hindering coccus lazy weight caused by coccus growth；

Packing: cow's milk culture medium containing lactic acid bacteria in desinfection chamber, is first dispensed into the vial of sale by step 9 Or in plastic small container, thermostatic chamber culture is sent into after capping, Yoghourt is made in fermentation in a reservoir.After cow's milk packing, upper vessel portion The gap reserved wants as small as possible, and the time of operation wants short；

Step 10, fermentation: the container equipped with lactobacteria-containing cow's milk being placed in insulating box and is fermented, the temperature of insulating box Degree is maintained at 40~43 DEG C, and the time is 3~4 hours；

Step 11, cooling: fermentation ends are answered before Yoghourt shifting to be put into refrigerating chamber and carries out storage and Aging storage Yoghourt is taken out from fermenting cellar, is cooled to 10 DEG C rapidly with cold wind hereinafter, the lactic acid bacteria in Yoghourt is made to stop growing；

Step 12, after-ripening: Yoghourt should be kept for 24 hours or more after forming grumeleuse at 4~7 DEG C；

Step 13, refrigeration: the Yoghourt through cooling treatment is housed in 2~5 DEG C, and preferably -1~0 DEG C of refrigerating chamber saves；

Step 14, taste: if there is smell, being likely to Yoghourt polluted miscellaneous bacteria.

Another object of the present invention is to provide a kind of functional yoghourt for treating abscess of throat, stirred yoghurt preparations Method, comprising:

Step 1, the processing of ingredient: preparation filtered juice.By Wan Niangan, purslane, frangipanis, peppermint and folium mori homogenate after It filters filter residue and takes juice, watermelon peel, Kiwi berry, pears and sugar cane crushing take juice.Each raw material is that (gross mass is by weight percentage 250g): Wan Niangan 5%~10%, radix polygonati officinalis 5%~8%, frangipanis 3%~5%, summer mushroom grass 3%~5%, purslane 3%~ 5%, (or Kiwi berry, pears, sugarcane, note: this ingredient is to have Yoghourt for peppermint 8%~15%, mulberry leaf 5%~10%, watermelon peel A series of different tastes) 6%~10%, starting milk 50%~65%, sucrose 3%~5%；

Milk bottle disinfection: step 2 milk bottle is used boiling water boiling 15 minutes in stainless-steel pan；

Step 3, the purification of cow's milk: utilizing specially designed centrifuge, removes the white blood cell in cow's milk and other visually may be used The foreign matter seen；

Normalising fat content: step 4 the skimmed milk of certain volume is added in the high cow's milk of fat content, or passes through Seperator isolates dilute cream from cow's milk, then mixes a certain amount of dilute cream again in obtained skimmed milk；

Ingredient: step 5 the sucrose of 4%-8% is added in modulation cream, raw milk is first heated to 60 DEG C or so, then Sucrose is added, after sugar dissolution, then filtering and impurity removing adds the filtered juice of step 1 preparation；

Step 6, homogeneous: the raw milk for making Yoghourt passes through homogenization, and butter oil is dispersed throughout.It will heating Get up processing with two methods of the appropriate combination of homogeneous, raw milk is first adjusted to 60 DEG C or so, then in homogenizer, in 8~ 10Mpa pressure carries out homogenization to cream；

Step 7, sterilizing: there are two types of the sterilizing methods of the raw milk after homogeneous, and cream is heated to 90 DEG C, keeps the temperature 5 minutes, Or it is placed in 80 DEG C of thermostat water baths and sterilizes 15 minutes；(2~3 can also be kept the temperature at 135 DEG C with ultra high temperature short time sterilization method Second)；

Step 8, inoculation: being added the working stock culture that inoculum concentration is 2~3% in the raw milk sterilized to 43~45 DEG C, For the Mixed Microbes of streptococcus thermophilus and lactobacillus fermenti, the ratio of two kinds of bacterium is 1:1 or 2:1.The inoculum concentration of coccus is slightly some more；

Step 9, fermentation: the container equipped with lactobacteria-containing cow's milk being placed in insulating box and is fermented, the temperature of insulating box Degree is maintained at 40~43 DEG C, and the time is 3~4 hours；

Step 10, stirring: being stirred into cup for the cow's milk containing lactic acid bacteria and contain in other containers, product curd particle diameter It is maintained at 0.01~0.04mm size；

Packing: cow's milk culture medium containing lactic acid bacteria in desinfection chamber, is first dispensed into the glass of sale by step 11 In bottle or plastic small container, thermostatic chamber culture is sent into after capping, Yoghourt is made in fermentation in a reservoir.After cow's milk packing, on container The gap that portion reserves wants as small as possible, and the time of operation wants short；

Step 12, cooling: fermentation ends are answered before Yoghourt shifting to be put into refrigerating chamber and carries out storage and Aging storage Yoghourt is taken out from fermenting cellar, is cooled to 10 DEG C or less rapidly with cold wind.

In conclusion advantages of the present invention and good effect are as follows:

1. the present invention by Wan Niangan, purslane, frangipanis, summer mushroom grass, mulberry leaf, radix polygonati officinalis, peppermint, watermelon peel, Kiwi berry, Filtered juice made of pears, sugarcane, which is added in milk, carries out the Yoghourt that fermentation preparation has treatment abscess of throat.Polysaccharide in sugarcane, ten thousand Galactolipin, arabinose, the xylose of Nian Ganzhong, the vitamin etc. in sugarcane can promote the proliferation of Lactis In Yoghurt, facilitate Yoghourt fermentation, and can increase the proliferation of the probiotics in the probiotics and enteron aisle in Yoghourt after drinkable yoghourt, while watermelon peel In dietary fiber can promote intestinal movement, promote toxin to excrete, improve intestinal microflora, facilitate probiotics inhibition Harmful bacteria has antiinflammation plant extraction filtrate synergistic effect with purslane, summer mushroom grass etc., significantly increases antiinflammation. On the other hand, mulberry-leaf extract 1-Deoxynojirimycin forms a kind of novel substance in conjunction with the flavones in radix polygonati officinalis, summer mushroom grass --- it washes The clear sugar of pancreas is plain (cics), has the effects that antibacterial anti-inflammatory；Mulberry-leaf extract inhibits the work of pathogenic bacteria under the acid condition of Yoghourt Property significantly increases.Kiwi berry rich in vitamin C facilitate it is antiviral, inhibit virus infection, enhance the immunity of body；It ties up simultaneously Raw element C has oxidation resistant function, and oxidized form of glutathione can be made to be reduced into reduced glutathione, plays antioxidation, The sterilizing ability for enhancing body neutrophil leucocyte, quickly inhibits inflammation.

2. 4~7 DEG C have the advantage that in low temperature After-ripening for low temperature after-ripening 24 hours, Yoghourt after Yoghourt fermentation solidification Fragrance matter gradually forms, and with the fragranced material mixing in plant extraction filtrate, armaticity flavor substance ratio is appropriate, makes acid Milk has compared with giving off a strong fragrance taste.And the acidity of Yoghourt rises few during after-ripening；Convergent force can be generated when Coagulation of milk, lead to whey It folds, in low temperature, this convergent force is weaker, so whey is not easy to separate from Yoghourt；Yoghourt gradually forms under low temperature Structural state as white jade, structure are very fine and smooth.

3. food-borne Chinese herbal medicine has many good qualities as additive: naturality, it is pollution-free and be conducive to environmental protection；It is multi-functional Property；Bidirectional modulation；Nontoxic secondary residual and nothing " three cause "；Without drug resistance；It is integrally-regulated and excitation transfer body in it is positive Factor.The Chinese herbal medicine substance that can alleviate abscess of throat is added in Yoghourt, Yoghourt and its inhibiting bacteria and diminishing inflammation ingredient is made to act synergistically, With effective specific function for inhibiting abscess of throat, diminishing inflammation, Yoghourt that such people can only by drinking Achieve the effect that treat abscess of throat, without spending many time to go to buy medicine and decocting medicinal herbs, does not have to the bitter taste for enduring drug also.

Detailed description of the invention

Fig. 1 is solidification type yoghourt preparation method process in the refrigerant Yoghourt provided in an embodiment of the present invention for controlling abscess of throat Figure.

Fig. 2 is stirred yoghurt production flow diagram provided in an embodiment of the present invention.

Specific embodiment

In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing Detailed description are as follows.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this Invention.

Zymophyte of the invention: lactobacillus fermenti (Lactobacillusfermentum), deposit number CGMCC No.8214 was deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation on 09 17th, 2013 Address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica.

Streptococcus thermophilus (Streptococcus thermophiles) of the invention, is purchased from Chinese microorganism strain preservation Administration committee's common micro-organisms center, address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences microorganism Research institute.

The refrigerant Yoghourt provided in an embodiment of the present invention for controlling abscess of throat, each raw material are that (gross mass is by weight percentage 250g): Wan Niangan 5%~10%, radix polygonati officinalis 5%~8%, frangipanis 3%~5%, summer mushroom grass 3%~5%, purslane 3%~ 5%, (or Kiwi berry, pears, sugarcane, note: this ingredient is to have Yoghourt for peppermint 8%~15%, mulberry leaf 5%~10%, watermelon peel A series of different tastes) 6%~10%, starting milk 50%~65%, sucrose 3%~5%, lactobacillus fermenti 6%~ 10% and streptococcus thermophilus 3%~9% form.This is that each constituent of Yoghourt accounts for the percentage of Yoghourt total weight, wherein fermenting Lactobacillus 6%~10% and streptococcus thermophilus 3%~9% are lactobacillus fermenti and streptococcus thermophilus by 1:1 or 2:1 Bijie with After the inoculum concentration inoculation of 2-3%, during fermented yoghourt, after both bacterium grown a period of time, each total weight of Yoghourt is accounted for Percentage.

As shown in Figure 1, solidification type yoghourt preparation method provided in an embodiment of the present invention, by Wan Niangan, purslane, egg Flower, peppermint and folium mori homogenate take juice after filtering filter residue, and watermelon peel, Kiwi berry, pears and sugar cane crushing take juice；

Specifically includes the following steps:

S101, the processing of ingredient: preparation filtered juice.Wan Niangan, purslane, frangipanis, peppermint and folium mori homogenate are refiltered Fall filter residue and take juice, watermelon peel, Kiwi berry, pears and sugar cane crushing take juice.Each raw material is (gross mass 250g) by weight percentage: It is Wan Niangan 5%~10%, radix polygonati officinalis 5%~8%, frangipanis 3%~5%, summer mushroom grass 3%~5%, purslane 3%~5%, thin (or Kiwi berry, pears, sugarcane, note: this ingredient is that have Yoghourt a series of for lotus 8%~15%, mulberry leaf 5%~10%, watermelon peel Different tastes) 6%~10%, starting milk 50%~65%, sucrose 3%~5%；

Milk bottle disinfection: S102 milk bottle is used boiling water boiling 15 minutes in stainless-steel pan；

S103, the purification of cow's milk: with specially designed centrifuge, the white blood cell removed in cow's milk is macroscopic with other Foreign matter；

Normalising fat content: the skimmed milk of certain volume is added, or by dividing in S104 in the high cow's milk of fat content It disembarks and isolates dilute cream from cow's milk, then mix a certain amount of dilute cream again in obtained skimmed milk；

S105, ingredient, the addition of sucrose: being heated to 60 DEG C or so for raw milk, and the sugarcane of 3%-5% is added in modulation cream Sugar, after sugar dissolution, then filtering and impurity removing adds the filtered juice of step 1 preparation；

Two methods of heating and the appropriate combination of homogeneous are got up to handle, raw milk are first adjusted to 60 DEG C of left sides by S106, homogeneous The right side carries out homogenization to cream in 8~10Mpa pressure then in homogenizer；

S107, sterilizing: there are two types of the sterilizing methods of the raw milk after homogeneous, and cream is heated to 90 DEG C, keeps the temperature 5min, or It is placed in 80 DEG C of thermostat water baths and sterilizes 15 minutes, or use ultra high temperature short time sterilization method (keeping the temperature 2~3s at 135 DEG C)；

Inoculation: S108 is added the working stock culture that inoculum concentration is 2~3%, is in the raw milk sterilized to 43~45 DEG C The Mixed Microbes of streptococcus thermophilus and lactobacillus fermenti, the ratio of two kinds of bacterium are 1:1 or 2:1.The inoculum concentration of coccus is slightly some more, can make up Since bacillus grows the shortcomings that producing acid and hindering coccus lazy weight caused by coccus growth；

S109, packing: in desinfection chamber, by the cow's milk culture medium containing lactic acid bacteria be first dispensed into sale vial or In plastic small container, thermostatic chamber culture is sent into after capping, Yoghourt is made in fermentation in a reservoir.After cow's milk packing, upper vessel portion is stayed Gap out wants as small as possible, and the time of operation wants short；

S110, fermentation: the container equipped with lactobacteria-containing cow's milk being placed in insulating box and is fermented, the temperature of insulating box 40~43 DEG C are maintained at, the time is 3~4 hours；

S111, cooling: fermentation ends should will be sour before Yoghourt shifting to be put into refrigerating chamber and carries out storage and Aging storage Milk takes out from fermenting cellar, is cooled to 10 DEG C rapidly with cold wind hereinafter, the lactic acid bacteria in Yoghourt is made to stop growing；

S112, after-ripening: Yoghourt should be kept for 24 hours or more after forming grumeleuse at 4~7 DEG C；

S113, refrigeration: the Yoghourt through cooling treatment is housed in 2~5 DEG C, and preferably -1~0 DEG C of refrigerating chamber saves；

S114, taste: if there is smell, being likely to Yoghourt polluted miscellaneous bacteria.

As shown in Fig. 2, stirred yoghurt preparation method provided in an embodiment of the present invention, specifically includes the following steps:

S201, the processing of ingredient: preparation filtered juice.Wan Niangan, purslane, frangipanis, peppermint and folium mori homogenate are refiltered Fall filter residue and take juice, watermelon peel, Kiwi berry, pears and sugar cane crushing take juice.Each raw material is (gross mass 250g) by weight percentage: It is Wan Niangan 5%~10%, radix polygonati officinalis 5%~8%, frangipanis 3%~5%, summer mushroom grass 3%~5%, purslane 3%~5%, thin (or Kiwi berry, pears, sugarcane, note: this ingredient is that have Yoghourt a series of for lotus 8%~15%, mulberry leaf 5%~10%, watermelon peel Different tastes) 6%~10%, starting milk 50%~65%, sucrose 3%~5%；

Milk bottle disinfection: S202 milk bottle is used boiling water boiling 15 minutes in stainless-steel pan；

S203, the purification of cow's milk: utilizing specially designed centrifuge, and the white blood cell removed in cow's milk is visible with other naked eyes Foreign matter；

Normalising fat content: the skimmed milk of certain volume is added, or by dividing in S204 in the high cow's milk of fat content It disembarks and isolates dilute cream from cow's milk, then mix a certain amount of dilute cream again in obtained skimmed milk；

S205, ingredient: being heated to 60 DEG C or so for raw milk, and the sucrose of 3%-5% is then added in modulation cream, and sugar is molten Then Xie Hou, filtering and impurity removing add the filtered juice of step 1 preparation；

Homogeneous: S206 two methods of heating and the appropriate combination of homogeneous is got up, raw milk is first adjusted to 60 DEG C or so, so Afterwards in homogenizer, homogenization is carried out to cream in 8~10Mpa pressure；

S207, sterilizing: there are two types of the sterilizing methods of the raw milk after homogeneous, and cream is heated to 90 DEG C, keeps the temperature 5min, or It is placed in 80 DEG C of thermostat water baths and sterilizes 15 minutes；Ultra high temperature short time sterilization method (2~3s is kept the temperature at 135 DEG C) can also be used；

Inoculation: S208 is added the working stock culture that inoculum concentration is 2~3%, is in the raw milk sterilized to 43~45 DEG C The ratio of the Mixed Microbes of streptococcus thermophilus and lactobacillus fermenti, two kinds of bacterium is 1:1 or 2:1, and the inoculum concentration of coccus is slightly some more；

S209, fermentation: the container equipped with lactobacteria-containing cow's milk being placed in insulating box and is fermented, the temperature of insulating box 40~43 DEG C are maintained at, the time is 3~4 hours；

S210, stirring: being stirred into cup for the cow's milk containing lactic acid bacteria and contain in other containers, and product curd particle diameter is protected It holds in 0.01~0.04mm size；

S211, packing: in desinfection chamber, by the cow's milk culture medium containing lactic acid bacteria be first dispensed into sale vial or In plastic small container, thermostatic chamber culture is sent into after capping, Yoghourt is made in fermentation in a reservoir.After cow's milk packing, upper vessel portion is stayed Gap out wants as small as possible, and the time of operation wants short；

S212, cooling: fermentation ends should will be sour before Yoghourt shifting to be put into refrigerating chamber and carries out storage and Aging storage Milk takes out from fermenting cellar, is cooled to 10 DEG C or less rapidly with cold wind.

Combined with specific embodiments below and validity check is further described application principle of the invention.

Embodiment 1

Vitro detection:

Macrophage be the innate immunity initial defence line cell and its adjust acquired immunity thereafter and have inducing Consequence.The Factor of Macrophage and inflammatory mediator of activation play an important role in generation, the development of inflammation, So the adjusting of macrophage activation has become some means and methods for preventing diseases associated with inflammation.

Cell is by secreting or adjusting inflammatory factor such as platelet activating factor TNF-α (tumor necrosis factor α), hs- CRP (hs-CRP) and IL-1 β (interleukin-1 beta) etc., to participate in, adjust, inducing inflammatory reaction.TNF-α is Obvious one of the cell factor of inflammatory reaction, while other inflammatory factors such as IL-1 β etc. can be induced again and generate coordination and made With.TNF-α, IL-1 β and hs-CRP have been widely used as test whether inflammation presence and its degree common index how.

The present invention is seen by establishing the external inflammatory model of lipopolysaccharides (LPS) stimulating expression of macrophage strain RAW246.7 activation Examine the anti-inflammatory effect for the treatment of abscess of throat Yoghourt.RAW264.7 cell is cultivated, Yoghourt extract is previously added and is incubated for 2 hours, after The LPS stimulation of final concentration of 200ng/ml is added, establishes cellular inflammation model, and establish sterile saline control group.LPS's Induction, can lead to the variation of relevant inflammatory factors such as TNF-α, IL-1 β and hs-CRP secretory volume, by measuring cell supernatant In various inflammatory factors secretory volume and cell in corresponding group express before and after modeling, to the change before and after Yoghourt extract Change, can preliminary judgement Yoghourt whether there is anti-inflammatory effect.

Below with reference to solution and preparation, the invention will be further described.

Main solution and preparation:

1,1640 culture medium: 13.4g DMEM dry-type culture medium is dissolved with 90ml ultrapure water, magnetic agitation keeps dry powder complete Fully dissolved adds 2.23g NaHCO₃And 3.08g Hepes, adjustment pH value add water to be settled to 1000ml to 7.2~7.4,0.22 μm It is dispensed after membrane filtration degerming, -20 DEG C of preservations.

2,0.25% trypsase: weighing trypsase 0.5g, is added in 200ml PBS, and magnetic agitation makes it completely Dissolution, dispenses after 0.22 μm of membrane filtration degerming, -20 DEG C of preservations.

3, Yoghourt extract: 100ml Yoghourt is taken to carry out refrigerated centrifuge (10000r/min, 10min), with 0.2 μm of filter membrane two Time, take supernatant.

Instrument:

Sorvall ST16R refrigerated centrifuge (Thermo Fishr Scientific company, the U.S.)；Mouse hs-CRP ELISA kit (R&D company, the U.S., lot number: 201207)；Mouse TNF-α ELISA kit (R&D company, the U.S., lot number: 201207)；Mouse IL-1 β ELISA kit (R&D company, the U.S., lot number: 201207).

Below with reference to cell culture, the invention will be further described.

1. cell culture

(1) source property macrophage strain RAW264.7 is incubated in RPMI1640 culture medium (containing 10% newborn bovine serum, 100 μ G/ml streptomysin, 100 μ/ml penicillin).Condition of culture is 37 DEG C, and gas concentration lwevel 5% is continuous to cultivate.

(2) 0.25% trypsin digestion of cell, cold culture medium is added after being rounded under the microscope and stops digestion, Centrifuging and taking precipitating is resuspended with culture medium after blowing and beating repeatedly, is inoculated in culture dish, is inoculated with initial density 2 × 10⁵/ ml, culture is extremely For testing after 80% fusion, serum-free processing in 12 hours makes cell synchronization before testing.

(3) Yoghourt extract is added by various concentration, is incubated for 2h in advance, 200ng/ml is added in solvent control DMSO afterwards LPS (final concentration) stimulate different time, take supernatant culture respectively, cell is used for different Indexs measures.

2.MTT colorimetric determination administers abscess of throat Yoghourt to cytotoxicity

Principle: the entitled thiazolyl blue of MTT quotient, to be faint yellow, it can be by the succinate dehydrogenase in living cells mitochondria also Original at slightly solubility bluish violet crystal violet object first a ceremonial jade-ladle, used in libation and be deposited in cell body, but MTT cannot be reduced to royal purple in dead cell Color crystal.The crystallization can be had at 560-610nm after dimethyl sulfoxide (DMSO) or the acidification dissolution such as isopropanol one compared with Wide maximum absorption peak can quantitative determine the light absorption at 570nm with microplate reader.Due to the production quantity and mitochondria of first a ceremonial jade-ladle, used in libation Succinate dehydrogenase activity is positively correlated, and can reflect dehydrogenase activity with the size of absorbance, to reflect living cells indirectly Quantity, living cells is more, and purple product is more, then light absorption (OD value) is higher.

Method: with 96 orifice plate culture cells, according to experimental group be added various concentration Yoghourt extract (0.8,4,20, 40,80 μM), at 37 DEG C, containing 5%CO₂Incubator in be incubated for 18h, be added the MTT of final concentration of 0.5mg/ml, be incubated for 4h Afterwards, it inhales and abandons supernatant, dimethyl sulfoxide (DMSO) is added in every hole, and shaken at room temperature 10min is sufficiently dissolved to purple crystal thing, uses Full-automatic microplate reader reads OD value at 570nm, every group of 6 hole of repetition.Calculate cell survival rate.Cell survival rate=(detection OD value/control wells OD value in hole) × 100%.

3. treating the influence (ELISA detection) that abscess of throat Yoghourt generates TNF-α to LPS stimulation RAW264.7 cell

(0.8,4,20 μM) is added by various concentration in Yoghourt extract, is incubated for 2h in advance, and other groups of addition solvents compare, LPS (final concentration) the stimulation 18h that liquid removes addition 200ng/ml after Yoghourt is changed, supernatant culture solution is taken respectively, is placed in -20 DEG C of preservations To detection.It is detected using the kit of commercialization, the concentration of culture solution TNF-α is groped in experiment in advance, to be diluted to kit Detection range.Each sample does multiple holes measurement.Determination step is as follows:

(1) sample 0.1ml is added in every hole, and ELISA Plate reacts 90 minutes plus lid, 37 DEG C.

(2) with the liquid got rid of in ELISA Plate after reacting, then against blotting paper clap it is several under, do not wash.

(3) ready biotin anti-TNF-α antibody working solution is sequentially added by every hole 0.1ml.37 DEG C are reacted 60 points Clock.

(4) every hole is added 0.01M PBS 0.35ml and washs 3 times, impregnates 1 minute or so every time.

(5) ready ABC working solution is sequentially added by every hole 0.1ml, (TMB blank develops the color except hole).37 DEG C anti- It answers 30 minutes.

(6) every hole is added 0.01M PBS 0.35ml and washs 5 times, impregnates 1~2 minute or so every time.

(7) it sequentially adds by every 90 μ l of hole in 37 DEG C of balances, 30 minutes TMB developing solutions, 37 DEG C are protected from light 15 points Clock.

(8) TMB terminate liquid is sequentially added by every hole 0.1ml, blue turns yellow at this time.OD is measured in 50nm with microplate reader Value.Corresponding concentration is calculated according to standard curve.

4. cell supernatant hs-CRP is detected

Using mouse hs-CRP ELISA kit, operated in strict accordance with kit specification, steps are as follows:

(1) dilution of standard items and sample-adding: 10 hole of standard sample wells is set on enzyme mark coating plate, is added respectively in sub 1,2 holes 100 μ l of standard items is mixed then in 50 μ l of the 1st, 2 Kong Zhongjia standard dilutions；Then 100 μ l points are respectively taken from the 1st, 2 holes It is not added to the 3rd, 4 holes, adds 50 μ l of standard dilutions respectively in the 3rd, 4 holes, mixes；Then 50 μ are respectively taken in the 3rd, 4 holes L is discarded, then respectively takes 50 to be added separately in the 5th, 6 holes, adds 50 μ l of standard dilutions respectively in the 5th, 6 holes, is mixed；From the 5th, It respectively takes 50 to be added separately in 7,8 holes in 6 holes, adds 50 μ l of standard dilutions respectively in 7,8 holes, mix；From the 7th, 8 holes 50 μ l are respectively taken to be added separately in the 9th, 10 holes, in the 9th, 10 holes respectively plus 50 μ l of standard dilutions is mixed, then the 9,50 μ l are respectively taken to discard in 10 holes.Each hole adds sample size to be 50 μ l after dilution, and concentration is respectively 6 μ l/L, 4 μ g/L, 2 μ g/L, 1 μg/L、0.5μg/L。

(2) it is loaded: white hole (sample and enzyme marking reagent being not added, remaining each step operation is identical), sample to be tested is respectively set Hole.Respectively add 40 μ l of sample diluting liquid in sample to be tested hole, then adds 10 μ l of sample to be tested (the final dilution of sample respectively again It is 5 times).Sample is added on microplate reader plate hole bottom by sample-adding, is not touched hole wall as far as possible, is shaked gently mixing.

(3) it incubates: being placed on 37 DEG C of electro-heating standing-temperature cultivator with sealing plate film sealing plate and incubate 30 minutes.

(4) match liquid: 30 (20 times of 48T) times concentrated cleaning solutions distilled water 30 (20 times of 48T) times is diluted into standby With.

(5) board-washing: taking out ELISA Plate, carefully throw off sealing plate film, discard liquid, dries, and board-washing liquid is filled it up in every hole, stands 30 It is discarded after second, is so repeated 5 times, pats dry.

(6) enzyme: every hole is added 50 μ l of enzyme marking reagent, except blank well.

(7) it incubates: being placed on 37 DEG C of electro-heating standing-temperature cultivator with sealing plate film sealing plate and incubate 30 minutes.

(8) board-washing: taking out ELISA Plate, carefully throw off sealing plate film, discard liquid, dries, and board-washing liquid is filled it up in every hole, stands 30 It is discarded after second, is so repeated 5 times, pats dry.

(9) develop the color: 50 μ l color developing agent A are first added in every hole, add 50 μ l color developing agent B, and gently concussion mixes, and 37 DEG C are protected from light Colour developing 15 minutes.

(10) terminate: every hole adds 50 μ l color development stopping of terminate liquid to react.

(11) it is returned to zero with blank well, 450nm wavelength sequentially measures each hole absorbance.15 minutes after measurement Ying Jia terminate liquid Within carry out.

(12) it calculates: according to the concentration of standard items and corresponding OD value, calculating the linear regression equation of standard curve, According to the OD value of sample, corresponding sample concentration is calculated on regression equation, ultimate density is actual concentrations multiplied by dilution times Number.

5. cell supernatant IL-1 β is detected

Using mouse IL-1 β ELISA kit, operated in strict accordance with kit specification, steps are as follows:

(1) dilution of standard items and sample-adding: 10 hole of standard sample wells is set on enzyme mark coating plate, is added respectively in sub 1,2 holes 100 μ l of standard items is mixed then in 50 μ l of the 1st, 2 Kong Zhongjia standard dilutions；Then 100 μ l points are respectively taken from the 1st, 2 holes It is not added to the 3rd, 4 holes, adds 50 μ l of standard dilutions respectively in the 3rd, 4 holes, mixes；Then 50 μ are respectively taken in the 3rd, 4 holes L is discarded, then respectively takes 50 to be added separately in the 5th, 6 holes, adds 50 μ l of standard dilutions respectively in the 5th, 6 holes, is mixed；From the 5th, It respectively takes 50 to be added separately in 7,8 holes in 6 holes, adds 50 μ l of standard dilutions respectively in 7,8 holes, mix；From the 7th, 8 holes 50 μ l are respectively taken to be added separately in the 9th, 10 holes, in the 9th, 10 holes respectively plus 50 μ l of standard dilutions is mixed, then the 9,50 μ l are respectively taken to discard in 10 holes.Each hole adds sample size to be 50 μ l after dilution, and concentration is respectively 90ng/L, 60ng/L, 30ng μ g/L、15ng/L、7.5ng/L。

(2) it is loaded: white hole (sample and enzyme marking reagent being not added, remaining each step operation is identical), sample to be tested is respectively set Hole.Respectively add 40 μ l of sample diluting liquid in sample to be tested hole, then adds 10 μ l of sample to be tested (the final dilution of sample respectively again It is 5 times).Sample is added on microplate reader plate hole bottom by sample-adding, is not touched hole wall as far as possible, is shaked gently mixing.

(3) it incubates: being placed on 37 DEG C of electro-heating standing-temperature cultivator with sealing plate film sealing plate and incubate 30 minutes.

(4) match liquid: 30 (20 times of 48T) times concentrated cleaning solutions distilled water 30 (20 times of 48T) times is diluted into standby With.

(5) board-washing: taking out ELISA Plate, carefully throw off sealing plate film, discard liquid, dries, and board-washing liquid is filled it up in every hole, stands 30 It is discarded after second, is so repeated 5 times, pats dry.

(6) 50 μ l of enzyme marking reagent is added in hole, except blank well.

(7) it incubates: being placed on 37 DEG C of electro-heating standing-temperature cultivator with sealing plate film sealing plate and incubate 30 minutes.

(8) board-washing: taking out ELISA Plate, carefully throw off sealing plate film, discard liquid, dries, and board-washing liquid is filled it up in every hole, stands 30 It is discarded after second, is so repeated 5 times, pats dry.

(9) develop the color: 50 μ l color developing agent A are first added in every hole, add 50 μ l color developing agent B, and gently concussion mixes, and 37 DEG C are protected from light Colour developing 15 minutes.

(10) hole adds 50 μ l color development stopping of terminate liquid to react.

(11) it is returned to zero with blank well, 450nm wavelength sequentially measures each hole absorbance.15 minutes after measurement Ying Jia terminate liquid Within carry out.

(12) it calculates: according to the concentration of standard items and corresponding OD value, calculating the linear regression equation of standard curve, According to the OD value of sample, corresponding sample concentration is calculated on regression equation, ultimate density is actual concentrations multiplied by dilution times Number.Compared with inflammatory model group, Yoghourt extract 1,2,3 can reduce the level of mouse TNF-α, IL-1 β and hs-CRP, thus Play the role of anti-inflammatory.Yoghourt extract doses are higher, and the difference moon is significant, and anti-inflammatory effects are more significant.

Each group TNF-α, hp-CRP, IL-1 β are horizontal

Below with reference to vivo detection, the invention will be further described.

Embodiment 2

Vivo detection:

Mouse method of inspection:

Mice caused by dimethylbenzene xylene auricle edema model

Mouse 40 is only randomly divided into 4 groups, i.e. physiological saline model group, Yoghourt extract low (125mg/kg), high dose Group (500mg/kg), positive control medicine (dexamethasone 10mg/kg), every group 10.Mouse is deprived of food but not water 12h before testing, Each group mouse presses 0.2ml/10g gastric infusion, and 1 time a day, total 7d, after the last administration 1h, each group mouse is in auris dextra tow sides It coats the dimethylbenzene (1.15ml/ only) that concentration is 100% and causes scorching, cause after inflammation 30min to sweep away left and right ear with punch same The disk at position using two auricle quality difference values as inflammation swelling, and calculates swelling inhibiting rate in weighing on assay balance: swollen Swollen inhibiting rate=(model group swelling-administration group swelling)/model group swelling × 100%.Yoghourt extract high concentration group To the swelling inhibiting rate of Mice Auricle up to 38.63%.

The influence of Yoghourt extract paraxylene cause mice auricle swelling

Treatment abscess of throat Yoghourt Shi Yingxian of the invention is eaten in mouth containing 5~10s, then is slowly swallowed.

Application principle of the invention is further described below with reference to pharmacological analysis.

Purslane of the invention is the young young stem and leaf of portulacaceous plant purslane, and China, various regions, north and south produce.Property is fertilizer favorite Fertile soil earth, drought-enduring also waterlogging, viability is strong, is born in vegetable garden, farmland, roadside, is field common weed.Purslane is clearing heat and detoxicating, Li Shui dries, and dissipates blood detumescence, dust-collection sterilization, anti-inflammatory analgetic, blood-stanching and blood-cooling.Cure mainly dysentery, enteritis, ephritis, postpartum uterine hemorrhage, Hematochezia, the illnesss such as mazoitis.

Frangipanis settles China and has centuries history, and just there is the frangipanis of one plant of more than 400 years of growth in Pinghu Town of Shenzhen Region street Ancient tree.The flower and bark of frangipanis can be used as medicine, can be clearing heat and detoxicating, moistening lung to arrest cough, in In Guangdong Province often by the chicken of white Egg flower, which is dried, makees antipyretic beverage, is popular, while flower can also extract aromatic oil, is used as modulation cosmetics and advanced perfume (or spice) Soap.According to the latest news, the sap of frangipanis can treat a kind of by skin caused by HIV infection or anticancer drug and electrotherapy Disease.Frangipanis is one of five flowers in the famous herbal tea five-flowered tea in Guangdong, cool in nature, sweet in flavor, light；Return large intestine, stomach meridian, there is moistening lung The effect of removing toxic substances, clearing heat and expelling damp, laxation.As " south of the Five Ridges gather medicinal herbs record " says that it " can control damp-heat diarrhea, inner urgency tongue weight, and energy moistening lung.

Summer mushroom grass is also known as ajuga decumbens, clarke boea herb, POXUE DAN.1 year or biennial herb, high 10~30cm.Prunella vulgaris For Lamiales Lamiaceae plant.It usually takes partial desiccation fruit ear to be used as medicine in summer, but makes in the common herb in TaiWan, China market With.Prunella vulgaris beverage all on sale is spread in general herbal tea.Mainly it is grown on sparse woods, deserted mountain, ridge and roadside, the month at florescence 4-6, fruit Month phase 7-10.Due to i.e. withered after this careless Summer Solstice, therefore there is this name.Bitter, acrid, cold.Enter liver, gallbladder channel.Its medical value has following several Point: red eye, swell pain, headache improving eyesight of 1. relieving inflammation or internal heat: are controlled.Prunella vulgaris 2. clearing away the liver-fire, blood pressure lowering: high blood pressure, hyperlipidemia, high blood viscosity are controlled And hyperglycemia.3. mass dissipating and swelling eliminating: controlling scrofula, goiter, acute mastitis swelling and pain.Clearing liver, dissipating bind, diuresis；Control seasonal febrile diseases, acute mastitis, mesh pain, Huang The diseases such as subcutaneous ulcer, stranguria syndrome, hypertension；Ye Kedai tea.

Radix polygonati officinalis is liliaceous plant.Elongated cylindrical, slightly flat, rare branch, surface yellow-white or fallow is translucent, Have the link of vertical wrinkle and micro- protuberance, there is the mark of fibrous root and discoid stem trace of white round point shape.The dry meat of radix polygonati officinalis is in root shape Stem is Polygonatum herbaceos perennial, is large Chinese medicine.Gas is micro-, sweet in flavor, the stickness chewed.Nourishing yin to moisten dryness is cured mainly, is promoted the production of body fluid only Yearningly.Hurt for lung stomach-Yin, hyperglycemia, cough caused by dryness-heat, dry throat and mouth, Heat Diabetes.

Mulberry leaf are the dried leafs of moraceae plants mulberry, are the conventional foods of silkworm.Mulberry-leaf extract 1-Deoxynojirimycin and summer mushroom Grass, which extracts flavonoid substances and combines, forms a kind of novel substance --- it is plain (cics) to wash the clear sugar of pancreas, suitable for control hypoglycemic, blood lipid, The effects of anti-inflammatory.Dispelling wind and heat from the body；Clearing lung-heat；Improving eyesight.Main anemopyretic cold；Wind-warm syndrome is from the beginning of, fever and headache, perspiration and aversion to wind, pectoralgia of coughing； Or dryness of the lung dry cough without phlegm；Dry throat and mouth；It is disturbed on wind-heat and liver-yang；Red eye, swell pain.For anemopyretic cold, lung-heat type cough, dizzy head Bitterly, red eyes and dim-sighted." Shandong Chinese medicine ": soothing sore throat, swelling and aching of gum, head edema.Clearing away the lungheat and moisturizing: be used for lung-heat type cough: the product are bitter Cold eliminating heat from the lung, sweet cold benefiting yin, cool moistening lung dryness, thus can be used for dry-heat impairing lung, dry cough lack mulberry-leaf extract 1-Deoxynojirimycin and Summer mushroom grass, which extracts flavonoid substances and combines, forms a kind of novel substance --- it is plain (cics) to wash the clear sugar of pancreas, suitable for control it is hypoglycemic, Blood lipid, it is anti-inflammatory the effects of.For hyperlipidemia, hyperglycemia, diabetes, fatty liver, coronary heart disease, hypertension, pharyngitis, first, B-mode liver The rehabilitation of the scorching postoperative chemicotherapy patient for the treatment of and cancer has extraordinary booster action.

Peppermint, alias HERBA MENTHAE.For Labiatae mint peppermint, (herbaceos perennial, stem have tetragonous, leaf pair Raw, Hua Chenghong, white or lavender, stem and leaf have refrigerant fragrance, can be used as medicine, or are used for food.It is pungent, cool, dispelling wind and heat from the body, Head clearing.Anemopyretic cold is cured mainly, is had a headache, abscess of throat is lossless, wind-fire red eye, rubeola, and skin, which is itched, waits diseases.Peppermint is virtue Fragrant compounds of group, peppermint oil and menthol are widely used in medicine, and are daily necessities and food industry primary raw material.This product lightly floats Rise floating, awakening consciousness, the kind evacuation part of the body cavity above the diaphragm housing the heart and lungs wind-heat of function, refresh oneself, throat.It is attacked in wind-heat with controlling, headache and hot eyes, polygamy closes mulberry Leaf, chrysanthemum, fructus viticis are equally used；Stop up Sheng with wind-heat is controlled, abscess of throat is often equivalent with campanulaceae, licorice, bombyx batryticatus, schizonepeta, radix saposhnikoviae With.

Watermelon peel, alias Mesocarpium citrulli.Source is the outside rind of cucurbitaceous plant watermelon.It gathers and processes 7~August and collects watermelon Skin is pruned internal layer soft, is dried；Also have to peel off on outside green peel and only take intermediate portion person.Character, dried fruit thin skin and Curling, not of uniform size at tubular or irregular shape, outer surface yellow, yellow green or dark brown；Inner surface has netted vascular bundle Strain line.Matter is crisp, frangibility.Remove outer layer green peel person, be in irregular strip and block, shrinkage and crimp, surface lark, have obvious Wrinkle and netted vascular bundle.Gas is micro-, lightly seasoned.The urea that citrulling contained in watermelon peel can be promoted in rats'liver is formed, thus It, can be to treat nephritic dropsy, hepatopathy jaundice and diabetes with diuresis.In addition there are antipyretic, promotion wound healings And promote effect of human skin metabolism.The clear heat of major function is antipyretic, quenches the thirst, diuresis.For hot summer weather polydipsia, urine Deficient, oedema, aphthae.

Soft, the sweet mouthfeel of Kiwi berry.Taste is described as the mixing of strawberry, banana, pineapple three.Kiwi berry Except containing the microelements such as organic matters and calcium, potassium, selenium, zinc, germanium such as actinidine, proteolytic enzyme, tannin pectin and carbohydrate Outside 17 kinds of amino acid of needed by human body, vitamin C also rich in, grape acid, fructose, citric acid, malic acid, fat.It can To treat dysphoria with smothery sensation, quench one's thirst, jaundice, vomiting, diarrhea, urolithiasis, the diseases such as arthralgia, and there are also the effects of anti-aging.Modern times doctor Research and analyse, and kiwifruit fruit contains carbohydrate, and Amino Acids in Proteins is abundant, and 12 kinds of protease, vitamin B1, C, Hu Luo The Multiple components such as Bu Su and calcium, phosphorus, iron, sodium, potassium, magnesium, chlorine, pigment.Its Vitamin C content is five to six in equivalent citrus Times.Kiwi berry acid, sweet in flavor cold, appropriate eat also has the effect of very effective fluid dryness, antipyretic relieving restlessness.For larynx Throat pain eats any fruit great question, and Kiwi berry is absolutely optimal selection.

Pears are rich in sugar, protein, fat, carbohydrate and multivitamin, play an important role, can change to human health Kind respiratory system and lung function, there is the effect of moistening lung, can reduce lung by air dust and flue dust influenced.Pears have life The effect of saliva quenches the thirst, is relieving cough and reducing sputum, heat-clearing and fire-reducing, therefore it is very suitable to the patients of winter fever and interior heat.It is especially right For the patient of the symptoms such as some autumn cough with lung heat, dry throat larynx pain, dry feces, autumn pear also has very effective help Digestion, antipyretic diuresis.In usually life, regardless of suitable a little autumn pears can be eaten either with or without symptoms such as sore throats more.

Sugarcane taste sweet and neutral, it is nontoxic.Main lower gas helps temper in, and profit large intestine, dissolving phlegm is quenched the thirst, and removes ambition dysphoria with smothery sensation, removing toxic substances.It is sweet Sugarcane energy purging intense heat heat, antipyretic to quench the thirst, He Zhongkuan diaphragm, fluid dryness help spleen stomach invigorating, diuresis, nourishing and other effects.It can be used for dry tongue Dry, body fluid deficiency, difficult urination, constipation, gastric disorder causing nausea vomiting, indigestion, has a fever thirsty.Drinking raw property of sugar-cane juice is sweet cold, fits Suitable consumption of body fluid caused by febrile disease, restlessness and thirst, body heat urinates red, xeropulmonary cough, and the raw sugarcane juice of febrile disease drink is best.Sphagitis patient eat raw with it is ripe Food all may be used.Prepared food sugar-cane juice gets up benefiting action to sphagitis patient.

The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form, Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to In the range of the technology of the present invention invention.

Claims (5)

1. a kind of refrigerant Yoghourt for controlling abscess of throat, which is characterized in that the refrigerant Yoghourt percentage by weight for controlling abscess of throat Than are as follows: Wan Niangan 5%~10%, radix polygonati officinalis 5%~8%, summer mushroom grass 3%~5%, frangipanis 3%~5%, purslane 3%~ 5%, peppermint 8%~15%, mulberry leaf 5%~10%, watermelon peel 6%~10%, starting milk 50%~65%, sucrose 3%~ 5%, lactobacillus fermenti 6%~10% and streptococcus thermophilus 3%~9% form.

2. a kind of solidification type yoghourt preparation method for the refrigerant Yoghourt for controlling abscess of throat as described in claim 1, which is characterized in that The solidification type yoghourt preparation method, comprising:

Step 1, the processing of ingredient: preparation filtered juice；By Wan Niangan, purslane, radix polygonati officinalis, summer mushroom grass, peppermint and folium mori homogenate after It filters filter residue and takes juice, watermelon peel, Kiwi berry, pears and sugar cane crushing take juice；Take each raw material by weight percentage: Wan Niangan 5%~ 10%, radix polygonati officinalis 5%~8%, frangipanis 3%~5%, summer mushroom grass 3%~5%, purslane 3%~5%, peppermint 8%~15%, Mulberry leaf 5%~10%, watermelon peel 7%~10%, starting milk 50%~65%, sucrose 3%~5%；

Milk bottle disinfection: step 2 milk bottle is used boiling water boiling 15 minutes in stainless-steel pan；

Step 3, the purification of cow's milk: with specially designed centrifuge, the white blood cell removed in cow's milk is macroscopic different with other Object；

Normalising fat content: step 4 the skimmed milk of certain volume is added in the high cow's milk of fat content, or passes through separation Machine isolates dilute cream from cow's milk, then mixes a certain amount of dilute cream again in obtained skimmed milk；

Step 5, ingredient: being heated to 60 DEG C or so for raw milk, and the sucrose of 3%-5% is added in modulation cream, after sugar dissolution, Then filtering and impurity removing adds the filtered juice of step 1 preparation；

Step 6, homogeneous: two methods of heating and the appropriate combination of homogeneous being got up to handle, raw milk is first adjusted to 60 DEG C or so, Then in homogenizer, homogenization is carried out to cream in 8~10Mpa pressure；

Step 7, sterilizing: there are two types of the sterilizing methods of the raw milk after homogeneous, and cream is heated to 90 DEG C, keeps the temperature 5 minutes, or set It sterilizes 15 minutes in 80 DEG C of thermostat water baths, or uses ultra high temperature short time sterilization method, keep the temperature 2~3 seconds at 135 DEG C；

Inoculation: working stock culture is added, inoculum concentration is 2~3%, thermophilic in step 8 in the raw milk sterilized to 43~45 DEG C The Mixed Microbes of streptococcus and lactobacillus fermenti, the ratio of two kinds of bacterium are 1:1 or 2:1；

Packing: cow's milk culture medium containing lactic acid bacteria in desinfection chamber, is first dispensed into vial or the modeling of sale by step 9 Expect in small container, thermostatic chamber culture is sent into after capping, and Yoghourt is made in fermentation in a reservoir；

Step 10, fermentation: the container equipped with lactobacteria-containing cow's milk being placed in insulating box and is fermented, the temperature 40 of insulating box ~43 DEG C, the time is 3~4 hours；

Step 11, cooling: fermentation ends should will be sour before Yoghourt shifting to be put into refrigerating chamber and carries out storage and Aging storage Milk takes out from fermenting cellar, is cooled to 10 DEG C rapidly with cold wind, and the lactic acid bacteria in Yoghourt is made to stop growing；

Step 12, after-ripening: Yoghourt should be kept for 24 hours after forming grumeleuse at 4~7 DEG C；

Step 13, refrigeration: the Yoghourt through cooling treatment is housed in 2~5 DEG C, and -1~0 DEG C of refrigerating chamber saves；

Step 14, taste: observation Yoghourt state tastes yogurt flavour.

3. a kind of stirred yoghurt preparation method for the refrigerant Yoghourt for controlling abscess of throat as described in claim 1, which is characterized in that The stirred yoghurt preparation method, comprising:

Step 1, the processing of ingredient: preparation filtered juice；By Wan Niangan, purslane, frangipanis, peppermint and folium mori homogenate after filtering Filter residue takes juice, and watermelon peel, Kiwi berry, pears and sugar cane crushing take juice；Take each raw material by weight percentage: Wan Niangan 5%~10%, Radix polygonati officinalis 5%~8%, frangipanis 3%~5%, summer mushroom grass 3%~5%, purslane 3%~5%, peppermint 8%~15%, mulberry leaf 5%~10%, watermelon peel 6%~10%, starting milk 50%~65%, sucrose 3%~5%；

Milk bottle disinfection: step 2 milk bottle is used boiling water boiling 15 minutes in stainless-steel pan；

Step 3, the purification of cow's milk: utilizing centrifuge, removes the foreign matter in cow's milk；

Normalising fat content: step 4 the skimmed milk of certain volume is added in the high cow's milk of fat content, or passes through separation Machine isolates dilute cream from cow's milk, then mixes a certain amount of dilute cream again in obtained skimmed milk；

Step 5, ingredient: being first heated to 60 DEG C for raw milk, and the sucrose of 3%-5%, sugar dissolution are then added in modulation cream Afterwards, then filtering and impurity removing adds the filtered juice of step 1 preparation；

Step 6, homogeneous: two methods of heating and the appropriate combination of homogeneous being got up to handle, raw milk is first adjusted to 60 DEG C or so, Then in homogenizer, homogenization is carried out to cream in 8~10Mpa pressure；

Sterilizing: cream is heated to 90 DEG C, keeps the temperature 5 minutes, or be placed in 80 DEG C of thermostat water baths and sterilize 15 minutes by step 7；Or 2~3 seconds are kept the temperature at 135 DEG C with ultra high temperature short time sterilization method；

Inoculation: working stock culture is added, inoculum concentration is 2~3%, thermophilic in step 8 in the raw milk sterilized to 43~45 DEG C The Mixed Microbes of streptococcus and lactobacillus fermenti, the ratio of two kinds of bacterium are 1:1 or 2:1；

Step 9, fermentation: the container equipped with lactobacteria-containing cow's milk being placed in insulating box and is fermented, the temperature 40 of insulating box ~43 DEG C, the time is 3~4 hours；

Step 10, stirring: being stirred into cup for the cow's milk containing lactic acid bacteria and contain in other containers, and curd particle diameter 0.01~ 0.04mm；

Step 11, packing: in desinfection chamber, by the cow's milk culture medium containing lactic acid bacteria be first dispensed into sale vial or In plastic small container, thermostatic chamber culture is sent into after capping, Yoghourt is made in fermentation in a reservoir；

Step 12, cooling: fermentation ends should will be sour before Yoghourt shifting to be put into refrigerating chamber and carries out storage and Aging storage Milk takes out from fermenting cellar, is cooled to 10 DEG C rapidly with cold wind.

4. a kind of refrigerant Yoghourt of children prepared using the refrigerant Yoghourt for controlling abscess of throat described in claim 1.

5. a kind of refrigerant Yoghourt of person in middle and old age prepared using the refrigerant Yoghourt for controlling abscess of throat described in claim 1.