CN109479881B - Application of 6 alpha-hydroxyureycolatone in antifeedant activity and development inhibition of insects - Google Patents
Application of 6 alpha-hydroxyureycolatone in antifeedant activity and development inhibition of insects Download PDFInfo
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- CN109479881B CN109479881B CN201811334238.2A CN201811334238A CN109479881B CN 109479881 B CN109479881 B CN 109479881B CN 201811334238 A CN201811334238 A CN 201811334238A CN 109479881 B CN109479881 B CN 109479881B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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Abstract
The invention discloses application of 6 alpha-hydroxyurea alkactone in antifeedant activity and growth inhibition of insects, which has good plant systemic property and conductance, has proliferation inhibition effect on insect germ cells, antifeedant activity on insects and growth inhibition of insects; the pesticide is obtained by separating and purifying natural plants, has low toxicity, no residue and no pollution to various economic crops such as grains, melons, fruits, vegetables and the like, can act on pests, and is more beneficial to environmental protection; can also be absorbed and metabolized by plants; has high antifeedant activity to insects, and can achieve the effect of preventing and controlling insect pests.
Description
Technical Field
The invention belongs to the field of biological pesticides and plant protection, and particularly relates to application of 6 alpha-hydroxyureycolatone in antifeedant activity and development inhibition of insects.
Background
The problem of pest control is always a problem which troubles agricultural production, and chemical pesticides are widely applied to agriculture, and although the chemical pesticides play an important role, a series of problems are introduced. Such as the development of pest resistance to drugs, environmental pollution, and adverse effects on natural enemies and non-target organisms.
The development of plant-derived pesticides is always a research hotspot in the field of pesticides because the plant-derived pesticides do not pollute the environment, are relatively safe to crops and ecological environment, and are not easy to generate drug resistance by insects. The insect antifeedant has all the characteristics of indirect insect killing, is efficient and nontoxic, and is an ideal botanical pesticide.
At present, the accurate use mechanism, the insect chemistry and physiology, the influence of the antifeedant on an ecological system and the like of the antifeedant are not clear, and if a safe and efficient insect antifeedant compound which can be transported in a plant body can be screened out for development and utilization, the method is an effective means for solving the problem.
Disclosure of Invention
The invention aims at providing the application of 6 alpha-hydroxyureycolatone in insect antifeedant.
Another object of the present invention is to provide the use of 6 α -hydroxyureycolatone for inhibiting the growth and development of insects.
The technical scheme adopted by the invention is as follows:
application of 6 alpha-hydroxyureycolatone in insect antifeedant.
Use of 6 alpha-hydroxyureycolatone for the preparation of a medicament having antifeedant activity against insects.
Application of 6 alpha-hydroxyureycolatone in preparing medicament with growth and development inhibiting activity on insects.
Further, the agent may be applied to the surface, root or/and stem of a plant leaf.
Further, the insects are at least one of lepidopteran insects and homopteran insects.
Further, the insects are at least one of insects in the families of noctuidae, plutella xylostella and aphididae.
Application of 6 alpha-hydroxyureycolatone in preparation of medicament for inhibiting proliferation of insect germ cells.
Furthermore, the insect germ cells are prodenia litura ovary cells and diamondback moth embryo cells.
An insect antifeedant capable of being introduced into plant contains 6 alpha-hydroxyureromalactone as active ingredient.
Further, the insects are at least one of plutella xylostella, noctuidae and aphididae.
Further, the insect preparation is emulsion, aqueous solution, aqueous emulsion, wettable powder and microemulsion.
A method for making insects antifeedant for plants is characterized by that the liquid medicine containing 6 alpha-hydroxyureycolatone is poured into a tank
Root or spray plant.
The invention has the beneficial effects that:
the 6 alpha-hydroxyureycolatone has good plant systemic property and delivery property, has the functions of inhibiting proliferation of insect germ cells, antifeedant activity on insects and inhibiting growth and development of the insects; the pesticide is obtained by separating and purifying natural plants, has low toxicity, no residue and no pollution to various economic crops such as grains, melons, fruits, vegetables and the like, can act on pests, and is more beneficial to environmental protection; can also be absorbed and metabolized by plants; has high antifeedant activity to insects, and can achieve the effect of preventing and controlling insect pests.
Detailed Description
The present invention will be further described with reference to the following examples.
The molecular formula of the 6 alpha-hydroxyureycolatone is C19H24O7。
Example 16 alpha-hydroxyuremycolone can be absorbed into the root through the root of the heart of the cabbage and conducted upwards to the leaves, causing insects to refuse to eat the leaves of the heart of the cabbage
The method comprises the following steps: dissolving 6 alpha-hydroxyureycomalactone in DMSO to obtain 10000 μ g/mL mother solution, and adding 0.5mM CaCl2The mother liquor was diluted to 100. mu.g/mL of culture broth with the aqueous solution. The previously cultivated untreated cabbage heart seedlings (6 leaf stage) were placed in 0.5mM CaCl2Pre-culturing in water solution for 2 hr, transferring into 100 μ g/mL prepared 6 α -hydroxyureycolatone culture solution, culturing for 24 hr, taking 0.7 g leaf of the Chinese flowering cabbage, mashing, dissolving in 2mL methanol, and detecting that the extractive solution contains 6 α -hydroxyureycolatone by using a liquid chromatography-mass spectrometer. The content of 6 alpha-hydroxyurea in the leaf of the top of the heart (fresh leaf) is calculated to reach 45 mu g/g. Beating the leaf of the top end of the cabbage heart absorbed with the 6 alpha-hydroxyurea mycolone into a circular leaf disc with the diameter of 1.2 cm, inoculating the 3 rd larva of the diamondback moth, and repeating for 3 times. Keeping the temperature of the mixture for 24 hours in an environment with the relative humidity of 50-70% and the temperature of 25-28 ℃, then counting the food intake area, and calculating the food intake rate and the food refusal rate.
The eating rate calculation formula is as follows:
the food refusal rate is calculated by the formula:
as a result: the 6 alpha-hydroxyureycolatone has better systemic property to plants, the antifeedant activity to the diamondback moth is detected by a butterfly method, and the 6 alpha-hydroxyureycolatone has obvious effect, and the antifeedant rate of the diamondback moth is higher than 85.2% after the plant is cultured in a culture solution containing the 6 alpha-hydroxyureycolatone for 48 hours, so that the 6 alpha-hydroxyureycolatone can be used as a good root-irrigation biological pesticide, has high antifeedant activity to insects, and can achieve the effect of preventing and treating plant diseases and insect pests.
Example 2: 6 alpha-hydroxyureycolatone has cell proliferation inhibiting effect on diamondback moth and prodenia litura
Whether the 6 alpha-hydroxyureycolatone has an inhibition effect on the proliferation of diamondback moth embryonic cells and prodenia litura ovarian cells or not is explored, the cell proliferation inhibition rate is detected by a CCK-8 method, and the results are shown in Table 1:
TABLE 16 inhibition of insect cell proliferation by alpha-hydroxyuremycotoxine
And (4) experimental conclusion: as can be seen from Table 1, 6 α -hydroxyureycolatone has a significant inhibitory effect on the proliferation of insect cells and is positively correlated with the concentration of 6 α -hydroxyureycolatone. The inhibition rates of the 6 alpha-hydroxyureycolatone with different concentrations on the diamondback moth embryonic cells are all more than 30 percent, and when the concentration is more than 6.25 mug/mL, the inhibition rates are all more than 50 percent;
the 6 alpha-hydroxyureycolatone also has strong inhibition effect on spodoptera litura ovarian cells; but the sensitivity to prodenia litura ovarian cells is slightly lower than that of diamondback moth embryonic cells; under the condition that the experimental concentration is more than 12.5 mu g/mL, the inhibition rate of the 6 alpha-hydroxyureycolatone on two insect cells is more than 50 percent.
Example 3: antifeedant activity of 6 alpha-hydroxyurectolone against insects
The method comprises the following steps: dissolving 6 alpha-hydroxyureycolatone in acetone to prepare a mother solution of 2000 mu g/mL, diluting the mother solution with acetone to obtain liquid medicines of 5 mu g/mL, 10 mu g/mL and 20 mu g/mL, uniformly coating the liquid medicines on the surface of a circular cabbage leaf disc with the diameter of 1.5 cm, coating the liquid medicine on each leaf disc to be 20 mu L, naturally drying the acetone on the surface of the leaf disc, then adding Plutella xylostella (Linnaeus) 3-year-old larvae, repeating the treatment for 3 times per concentration, counting the feeding area after keeping the temperature for 24 hours in an environment with the relative humidity of 50-70% and the temperature of 25-28 ℃, and calculating the feeding rate and the food refusal rate, wherein the calculation formula is shown in example 1.
Cassava leaf for testing the antifeedant activity of 6 alpha-hydroxyurectolone on Spodopteritaura (Fabricius)1 st instar larvae; amaranth leaf blades were used to test 6 α -hydroxyureycolatone for antifeedant activity against 1 st instar terminal larvae of spodopteragegus exigua (hubner); when the anti-feeding activity of the 6 alpha-hydroxyuremycolone on the Lipaphinerysimi is tested, the leaf of the cabbage heart is used, and other processing methods and calculation methods are the same as those of the 3 rd larva of the diamondback moth.
TABLE 26 antifeedant Activity of alpha-hydroxyuremycotoxines against insect larvae (48 h after treatment)
TABLE 36 antifeedant concentrations of alpha-hydroxyureromobacterone and Azadirachtin for insects
As a result: the antifeedant rate of the insects after the 6 alpha-hydroxyureycolatone is treated for 48 hours is shown in tables 2 and 3, and the 6 alpha-hydroxyureycolatone has antifeedant activities with different degrees on asparagus caterpillar, diamond back moth, prodenia litura and cabbage aphid; the antifeedant concentration of the 6 alpha-hydroxyureycolatone to the asparagus caterpillars is 6.13 mu g/g, which is lower than that of the Azadirachtin of a control group; antifeedant effect of 6 alpha-hydroxyureycolatone on insects under the same conditions: beet armyworm, plutella xylostella, cabbage aphid and prodenia litura, but all show higher antifeedant activity.
Example 4: the compound has inhibitory activity on insect growth
The method comprises the following steps: adding 6 alpha-hydroxyureycolatone with the concentration of 5 mu g/g into feed, and feeding insects for 48 hours, wherein diamondback moth is larva at the middle stage of 3 instars, and beet armyworm is larva at the later stage of 2 instars; feeding normal feed to a control group; sampling and detecting the weight of the insect after feeding for 48 hours; and (4) feeding the 3 rd instar diamondback moth larvae for 8 days, and feeding the 2 nd instar beet armyworm larvae for 10 days, and then calculating the pupation rate.
TABLE 46 Effect of alpha-hydroxyuremycotoxines on insect growth
TABLE 56 Effect of alpha-hydroxyureycolatone on insect pupation Rate
As a result: as shown in tables 4 and 5, both 6 α -hydroxyureycolatone showed significant growth inhibition against diamond back moth and beet armyworm, and the weight of the single insect was reduced by 0.006g to 0.024g compared with the control group. Compared with the control group, the pupation rate of the diamondback moth and the beet armyworm after eating the 6 alpha-hydroxyureycomilactone is obviously lower than that of the control group.
The above description is a preferred embodiment of the present invention, but the present invention is not limited to the above embodiment, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and they are included in the scope of the present invention.
Claims (5)
1.6 application of alpha-hydroxyureycolatone in preparing medicament with antifeedant activity to insects;
wherein the concentration of the 6 alpha-hydroxyureycolatone is more than 5 mu g/g;
the insects are at least one of lepidoptera insects and homoptera insects.
2.6 application of alpha-hydroxyureycolatone in insect antifeedant;
wherein the concentration of the 6 alpha-hydroxyureycolatone is more than 5 mu g/g;
the insects are at least one of lepidoptera insects and homoptera insects.
3.6 application of alpha-hydroxyureycolatone in preparing medicaments with growth and development inhibiting activity on insects;
wherein the concentration of the 6 alpha-hydroxyureycolatone is more than 5 mu g/g;
the insects are at least one of lepidoptera insects and homoptera insects.
4.6 application of alpha-hydroxyureycolatone in preparing a medicament for inhibiting the proliferation of insect germ cells;
wherein the concentration of the 6 alpha-hydroxyureycolatone is more than 12.5 mu g/mL;
the insect germ cells are prodenia litura ovary cells and diamondback moth embryo cells.
5. A method for making insects to refuse to eat plants is characterized in that a liquid medicine containing 6 alpha-hydroxyureycolatone is used for irrigating roots or spraying plants;
the insects are at least one of lepidoptera insects and homoptera insects;
the concentration of the 6 alpha-hydroxyureycolatone is more than 5 mu g/g.
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| CN113975268B (en) * | 2021-10-26 | 2023-11-24 | 广东中诚生物科技有限公司 | Application of 5, 6-dehydroeurycommalone in preparation of anti-dengue virus drugs |
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