CN109477086A - 一种基因组dna特异性编辑方法和应用 - Google Patents
一种基因组dna特异性编辑方法和应用 Download PDFInfo
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Abstract
一种调节核酸的甲基化/去甲基化状态的方法,具体为:一种可以在细胞内由sgRNA序列引导的在基因组上定点去除一个或多个甲基化碱基的方法。
Description
PCT国内申请,说明书已公开。
Claims (15)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610550293 | 2016-07-13 | ||
CN201610550293X | 2016-07-13 | ||
PCT/CN2017/088281 WO2018010516A1 (zh) | 2016-07-13 | 2017-06-14 | 一种基因组dna特异性编辑方法和应用 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN109477086A true CN109477086A (zh) | 2019-03-15 |
Family
ID=60952707
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201780043459.1A Pending CN109477086A (zh) | 2016-07-13 | 2017-06-14 | 一种基因组dna特异性编辑方法和应用 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20230151341A1 (zh) |
CN (1) | CN109477086A (zh) |
WO (1) | WO2018010516A1 (zh) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019041296A1 (zh) * | 2017-09-01 | 2019-03-07 | 上海科技大学 | 一种碱基编辑系统及方法 |
CN109021111B (zh) * | 2018-02-23 | 2021-12-07 | 上海科技大学 | 一种基因碱基编辑器 |
WO2019161783A1 (en) * | 2018-02-23 | 2019-08-29 | Shanghaitech University | Fusion proteins for base editing |
CN108753823B (zh) * | 2018-06-20 | 2022-09-23 | 李广磊 | 利用碱基编辑技术实现基因敲除的方法及其应用 |
CN111165342A (zh) * | 2020-01-19 | 2020-05-19 | 安徽省农业科学院水稻研究所 | 一种偏籼型水稻恢复系的选育方法 |
CN114540325B (zh) * | 2022-01-17 | 2022-12-09 | 广州医科大学 | 靶向dna去甲基化的方法、融合蛋白及其应用 |
WO2023155901A1 (en) * | 2022-02-17 | 2023-08-24 | Correctsequence Therapeutics | Mutant cytidine deaminases with improved editing precision |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015089406A1 (en) * | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Cas variants for gene editing |
WO2015133554A1 (ja) * | 2014-03-05 | 2015-09-11 | 国立大学法人神戸大学 | 標的化したdna配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体 |
CN105112446A (zh) * | 2015-06-25 | 2015-12-02 | 中国医学科学院基础医学研究所 | 使用单倍体干细胞高效建立遗传修饰动物模型的方法 |
WO2016025131A1 (en) * | 2014-08-13 | 2016-02-18 | E. I. Du Pont De Nemours And Company | Genetic targeting in non-conventional yeast using an rna-guided endonuclease |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3988667A1 (en) * | 2013-03-15 | 2022-04-27 | The General Hospital Corporation | Using truncated guide rnas (tru-grnas) to increase specificity for rna-guided genome editing |
-
2017
- 2017-06-14 WO PCT/CN2017/088281 patent/WO2018010516A1/zh active Application Filing
- 2017-06-14 CN CN201780043459.1A patent/CN109477086A/zh active Pending
- 2017-06-14 US US16/317,524 patent/US20230151341A1/en not_active Abandoned
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015089406A1 (en) * | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Cas variants for gene editing |
WO2015133554A1 (ja) * | 2014-03-05 | 2015-09-11 | 国立大学法人神戸大学 | 標的化したdna配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体 |
WO2016025131A1 (en) * | 2014-08-13 | 2016-02-18 | E. I. Du Pont De Nemours And Company | Genetic targeting in non-conventional yeast using an rna-guided endonuclease |
CN105112446A (zh) * | 2015-06-25 | 2015-12-02 | 中国医学科学院基础医学研究所 | 使用单倍体干细胞高效建立遗传修饰动物模型的方法 |
Non-Patent Citations (4)
Title |
---|
BINYAMIN A. KNISBACHER等: "DNA Editing by APOBECs: A Genomic Preserver and Transformer", 《TRENDS IN GENETICS》 * |
MARTA VAZQUEZ‑VILAR等: "A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard", 《PLANT METHODS》 * |
PRIYANGA WIJESINGHE等: "Efficient deamination of 5-methylcytosines in DNA by human APOBEC3A, but not by AID or APOBEC3G", 《NUCLEIC ACIDS RESEARCH》 * |
阴晓彤等: "哺乳动物DNA甲基化修饰的动态调控机制", 《生命的化学》 * |
Also Published As
Publication number | Publication date |
---|---|
US20230151341A1 (en) | 2023-05-18 |
WO2018010516A1 (zh) | 2018-01-18 |
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