CN109470875A - A kind of blood cell analysis dilution - Google Patents
A kind of blood cell analysis dilution Download PDFInfo
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- CN109470875A CN109470875A CN201710798775.1A CN201710798775A CN109470875A CN 109470875 A CN109470875 A CN 109470875A CN 201710798775 A CN201710798775 A CN 201710798775A CN 109470875 A CN109470875 A CN 109470875A
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- dilution
- blood cell
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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Abstract
The invention discloses a kind of blood cell analysis dilutions, in 1L dilution: 4.0~27.0g of potassium chloride, 0.04~6.0g of EDTAP dipotassium ethylene diamine tetraacetate, and pyridine 0.02~3.8g of sodium, 18~22g of potassium dihydrogen phosphate, 9~12g of sodium hydroxide, surplus is purified water.The present invention cooperates fully automatic blood cytoanalyze to use, not damaged to instrument, and without preservative, without cyanide in reagent, no pollution to the environment is discharged waste liquid and is not required to harmless treatment, can be in wide clinical application.
Description
Technical field
Invention belongs to external diagnosis reagent field, and in particular to a kind of blood cell analysis dilution.
Background technique
Dilution and hemolytic agent are the most common reagents of blood analyser in medical test, are measurements hemoglobin (HGB)
The key reagents for counting or classifying with leucocyte (WBC).On the one hand hemolytic agent makes red blood cell under the mating reaction of dilution
(RBC) dissolution rapidly, releases hemoglobin and forms stable haemoglobin dervative in conjunction with hemolytic agent, to provide measurement
Determine HGB；Another aspect leucocyte maintains original form, and keeps certain volume, counts for WBC, and according to white thin
The size of born of the same parents to carry out three points of groups: lymphocyte (LYM), monocyte (Mo), granulocyte (GRA) to leucocyte.
In general, blood cell analysis is to use diluted blood sample, then it is red to measure to have diluted blood sample for analysis a part
Cell and platelet parameter, then remaining is added in diluted blood sample in hemolytic agent, go out red blood cell and blood platelet, to carry out
Content of hemoglobin measurement, the measurement of white blood count and differential.
Since red blood cell, leucocyte, blood platelet and hemoglobin concentration are to the sensibility of chemical reagent, it is difficult to find one kind
Both degradation, which occurs, will not undesirably change the combination of chemical reagent of the cell characteristics and hemoglobin parameters that need to analyze,
Especially under conditions of changing operation temperature.The physical parameter of cell will receive the influence of diluent ingredient and analysis method,
Either automatic, semi-automatic or hand-manipulated, existing dilution, which all defines, must use special experimental method or use
Proprietary instrument, therefore, existing dilution is cannot be general or for kinds of experiments instrument or experimental method.And current state
Contain in the inside and outside dilution component being widely used to the prejudicial ingredient of blood analyser, and the cyaniding strong containing toxicity
Object jeopardizes human health and causes problem of environmental pollution.
The present invention is developed on the basis of mainly solving currently available technology and having above insufficient.
Summary of the invention
Present invention aim to address the above technical problem, a kind of cooperation fully automatic blood cytoanalyze especially set out makes
With, it is not damaged to instrument, and without preservative, without cyanide in reagent, no pollution to the environment, discharge waste liquid is not required to harmless
Change processing, can be in the blood cell analysis dilution of wide clinical application.Based on above-mentioned purpose, technology solved by the invention is asked
Topic is realized using following technical scheme:
A kind of blood cell analysis dilution, which is characterized in that in 1L dilution: 4.0~27.0g of potassium chloride, second two
Amine tetraacethyl 0.04~6.0g of dipotassium, pyridine 0.02~3.8g of sodium, 15~22g of potassium dihydrogen phosphate, sodium hydroxide 6~
12g, surplus are purified water.
Optimal, in 1L dilution: potassium chloride 19g, EDTAP dipotassium ethylene diamine tetraacetate 4.8g, pyridine sodium 2.6g, phosphoric acid
Potassium dihydrogen 17g, sodium hydroxide 9g, surplus are purified water.
The technical principle of instant component:
Pyridine sodium: phase transfer catalyst.
Potassium chloride: being a kind of osmotic pressure maintenance agent.
EDTAP dipotassium ethylene diamine tetraacetate: protease, nucleic acid inhibitor, blood anticoagulant.
Potassium dihydrogen phosphate, sodium hydroxide: PH buffer.
Purified water: dilute solution in allotment.
The beneficial effects of the present invention are: cooperation fully automatic blood cytoanalyze uses, it is not damaged to instrument, and reagent
In without preservative, be free of cyanide, no pollution to the environment, discharge waste liquid be not required to harmless treatment, can be in wide clinical application.
Specific embodiment
Below by way of specific embodiment, the present invention is further explained.
A kind of blood cell analysis dilution, in 1L dilution: 4.0~27.0g of potassium chloride, ethylenediamine tetra-acetic acid
0.04~6.0g of dipotassium, pyridine 0.02~3.8g of sodium, 18~22g of potassium dihydrogen phosphate, 9~12g of sodium hydroxide, surplus
For purified water.
Embodiment 1, in 1L dilution: potassium chloride 19g, EDTAP dipotassium ethylene diamine tetraacetate 4.8g, pyridine sodium 2.6g, phosphorus
Acid dihydride potassium 17g, sodium hydroxide 9g, surplus is purified water, and above-mentioned each component uniform stirring is allowed to dissolve.
The blood cell analysis dilution made by above embodiments, cooperation fully automatic blood cytoanalyze make
With, it is not damaged to instrument, and without preservative, without cyanide in reagent, no pollution to the environment, discharge waste liquid is not required to harmless
Change processing, can be in wide clinical application.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit application field of the present invention.It is all in the present invention
Any modifications, equivalent replacements, and improvements etc. done within spirit and principle, should all be included in the protection scope of the present invention.
Claims (2)
1. a kind of blood cell analysis dilution, which is characterized in that in 1L dilution: 4.0~27.0g of potassium chloride, second
Ethylenediamine tetraacetic acid (EDTA) 0.04~6.0g of dipotassium, pyridine 0.02~3.8g of sodium, 15~22g of potassium dihydrogen phosphate, sodium hydroxide 6
~12g, surplus are purified water.
2. blood cell analysis dilution according to claim 1, which is characterized in that in 1L dilution: potassium chloride
19g, EDTAP dipotassium ethylene diamine tetraacetate 4.8g, pyridine sodium 2.6g, potassium dihydrogen phosphate 17g, sodium hydroxide 9g, surplus is purified water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710798775.1A CN109470875A (en) | 2017-09-07 | 2017-09-07 | A kind of blood cell analysis dilution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710798775.1A CN109470875A (en) | 2017-09-07 | 2017-09-07 | A kind of blood cell analysis dilution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109470875A true CN109470875A (en) | 2019-03-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710798775.1A Pending CN109470875A (en) | 2017-09-07 | 2017-09-07 | A kind of blood cell analysis dilution |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022001825A1 (en) * | 2020-07-01 | 2022-01-06 | 天津市肿瘤医院（天津医科大学肿瘤医院） | Kit for detecting e-cadherin expression of peripheral blood circulating tumor cells of pancreatic cancer patient and detection method |
-
2017
- 2017-09-07 CN CN201710798775.1A patent/CN109470875A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022001825A1 (en) * | 2020-07-01 | 2022-01-06 | 天津市肿瘤医院（天津医科大学肿瘤医院） | Kit for detecting e-cadherin expression of peripheral blood circulating tumor cells of pancreatic cancer patient and detection method |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190315 |