CN109453258A - A kind of radix rehmanniae and preparation method thereof - Google Patents
A kind of radix rehmanniae and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to technical field of traditional Chinese medicine preparation, in particular to a kind of radix rehmanniae and preparation method thereof.The present invention prepares radix rehmanniae, radix rehmanniae catalpol content >=79.35mgg obtained using vitamin C aqueous solution spray glutinous rehmannia fresh rhizome slice‑1, have with the comparable clearing heat and promoting fluid effect of fresh rehmannia root, and remain the color of fresh rehmannia root.Experiment shows that the radix rehmanniae can be obviously improved yeast and LPS causes Na in fever model rat blood serum+‑K+ATP enzyme, Ca2+‑Mg2+The content of ATP enzyme, lactic dehydrogenase (SDH), succinate dehydrogenase (LDH), interleukin-6 (IL-6) and interleukin-8 (IL-8), has good refrigeration function, and therapeutic effect is suitable with fresh rehmannia root juice.Preparation method of the present invention is easy to operate, high-efficient, process stabilizing, low in cost, facilitates storage and transport, is suitable for industrialized production.
Description
Technical field
The present invention relates to field of traditional Chinese medicine preparations more particularly to a kind of radix rehmanniae and preparation method thereof.
Background technique
Glutinous rehmannia is listed in top grade first recorded in Shennong's Herbal, is scrophulariaceae rehmannia glutinosa plant Rehmannia glutinosa
Libosch. fresh or dried root.Autumn excavation, removes removing LU, fibrous root and silt, using fresh herb, or glutinous rehmannia slowly baked to
It is most probably dry.The former practises title " fresh rehmannia root ", the latter practise claim " radix rehmanniae recen ", fresh rehmannia root can clearing heat and promoting fluid, cool blood, hemostasis, radix rehmanniae recen is then
With clearing heat and cooling blood, the effect of nourishing Yin and promoting production of body fluid.Radix Rehmanniae ecliptic longitude can be made into Rehmannia glutinosa after steaming, flavor efficacy generation obviously changes
Become, is chiefly used in enriching yin of enriching blood, beneficial to spirit and marrow.
In fact, the processed product that the successive dynasties record glutinous rehmannia has fresh rehmannia root to twist juice, net system, cutting, wine system, frying, charcoal processing, vinegar
A variety of methods such as system, ginger system, salt system, sweet system, fructus amomi system, cooking and decoction.Modern times, although various methods are simplified,
But still net system fresh rehmannia root, dry radix rehmanniae recen and steamed legal system Rehmannia glutinosa and the stewed legal system Rehmannia glutinosa of wine are retained.
Fresh rehmannia root juice have good clearing heat and cooling blood effect, patent CN101040958A " fresh rehmannia root juice for treating with prevented
The medical usage of quick property disease " just specifies fresh rehmannia root juice with the small equal spies of anti-inflammatory, antianaphylactic activity and toxic side effect
Point.Fresh rehmannia root color cadmium yellow, but color change can occur in the drying process that radix rehmanniae recen is made, become sepia from vivid,
At the same time, the content of effective component Catalpol and acteoside is also decreased obviously, in drug action also from clearing heat and promoting fluid toward
In phencyclidine.
The freeze-drying radix rehmanniae technology of prior art report, at present in color and the active constituent content side for retaining fresh rehmannia root
Face has certain advantage.But the technology is higher to equipment and personnel requirement, is not easy to the popularization and industrial metaplasia of large area
It produces, and makees data supporting without related drug efficacy study, clinical efficacy needs to be confirmed.
Therefore, develop it is a kind of it is simple to operation, lower to equipment and personnel requirement, can retain suitable for what is be widely applied
The preparation process of the effect of fresh rehmannia root juice clearing heat and promoting fluid and former coloured radix rehmanniae becomes new issue urgently to be resolved at present.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of radix rehmanniae and preparation method thereof, radix rehmanniae of the present invention
Preparation process is easy to operate, high-efficient, process stabilizing lower to equipment and personnel requirement, low in cost, glutinous rehmannia drink obtained
Piece have with the comparable clearing heat and promoting fluid effect of fresh rehmannia root, and remain the color of fresh rehmannia root.
In order to achieve the object of the present invention, the present invention adopts the following technical scheme:
The present invention provides a kind of radix rehmanniae, catalpol contents >=79.35mgg-1。
Further, acteoside content >=7.11mgg in the radix rehmanniae-1。
The present invention provides a kind of preparation methods of radix rehmanniae, include the following steps:
Step 1: glutinous rehmannia fresh rhizome being taken to be sliced；
Step 2: by slice made from step 1, uniformly spray vitamin C aqueous solution, forced air drying to water content are lower than
13%, obtain radix rehmanniae.
Step 1 of the present invention is sliced using glutinous rehmannia fresh rhizome as raw material, further includes by glutinous rehmannia fresh rhizome before slice
It cleans, removes the pre-treatment step of putrid and deteriorated part.
In some embodiments, the slice with a thickness of 2-8mm.
In certain embodiments, the slice with a thickness of 4-6mm.
In some embodiments, forced air drying is also further included the steps that after the slice, the forced air drying
Time is 0.5~2 hour, and the temperature of forced air drying is 40 DEG C.
In certain embodiments, the time of the forced air drying is 1 hour, and the temperature of forced air drying is 40 DEG C.
Step 2 of the present invention is lower than using the slice obtained of vitamin C aqueous solution spraying step 1, forced air drying to water content
13%, obtain radix rehmanniae.Firstly, preparing vitamin C aqueous solution using pure water.In some embodiments, vitamin C is water-soluble
The concentration of liquid is 0.5-2mg/mL.
In certain embodiments, the concentration of vitamin C aqueous solution is 1mg/mL.
In some embodiments, the spray is water-soluble with 0.5-2L vitamin C according to every 100kg glutinous rehmannia fresh rhizome
Liquid is sprayed.
In certain embodiments, it is sprayed according to every 100kg glutinous rehmannia fresh rhizome with 1L vitamin C aqueous solution.
In some embodiments, the temperature of step 2 forced air drying is 40 DEG C.
The present invention also provides the radix rehmanniaes that above-mentioned method obtains.
In a specific embodiment, the present invention has investigated the clearing heat and promoting fluid effect for the radix rehmanniae that the present invention produces, knot
Fruit shows that the radix rehmanniae that the present invention produces can be obviously improved yeast and LPS causes Na in fever model rat blood serum+-K+ATP enzyme, Ca2+-Mg2+ATP enzyme, lactic dehydrogenase (SDH), succinate dehydrogenase (LDH), interleukin-6 (IL-6) Ji Baijie
The content of -8 (IL-8) of element, the effect with fresh rehmannia root juice is without significant difference.The result shows that the present invention is made with significantly antipyretic
Effect, therapeutic effect are suitable with fresh rehmannia root juice.Therefore the present invention also provides the radix rehmanniaes in treatment fever due to yin deficiency
Application in drug.
Compared with glutinous rehmannia blade technolgy is lyophilized in the prior art, the present invention sprays glutinous rehmannia fresh rhizome using vitamin C aqueous solution
Slice prepares radix rehmanniae, and preparation process is simple to operation, lower to equipment and personnel requirement, and has high-efficient, technique
It is stable, low in cost, the advantages that facilitating storage and transport, it is suitable for industrialized production.Radix rehmanniae Catalpol produced by the present invention contains
Amount >=79.35mgg-1, not only remain the color of fresh rehmannia root, and have with the comparable clearing heat and promoting fluid effect of fresh rehmannia root, have
Effective component content and clearing heat and promoting fluid effect are substantially better than freeze-drying rehmannia tablets.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described.
Fig. 1 is the chromatogram of Catalpol standard items under 2.1 chromatographic conditions in embodiment 9；
Fig. 2 is the chromatogram of fresh rehmannia root under 2.1 chromatographic conditions in embodiment 9；
Fig. 3 is the chromatogram of 1 radix rehmanniae of embodiment under 2.1 chromatographic conditions in embodiment 9；
Fig. 4 is the chromatogram of dry radix rehmanniae recen under 2.1 chromatographic conditions in embodiment 9；
Fig. 5 is the chromatogram of acteoside standard items under 2.2 chromatographic conditions in embodiment 9；
Fig. 6 is the chromatogram of fresh rehmannia root under 2.2 chromatographic conditions in embodiment 9；
Fig. 7 is the chromatogram of 1 radix rehmanniae of embodiment under 2.2 chromatographic conditions in embodiment 9；
Fig. 8 is the chromatogram of dry radix rehmanniae recen under 2.2 chromatographic conditions in embodiment 9.
Specific embodiment
The invention discloses a kind of radix rehmanniae and preparation method thereof, those skilled in the art can use for reference present disclosure,
It is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications carry out those skilled in the art
Say it is it will be apparent that they are considered as being included in the present invention.Method and application of the invention has passed through preferred embodiment
It is described, related personnel can obviously not depart from the content of present invention, in spirit and scope to method described herein and answer
With being modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
To the explanation of the disclosed embodiments, enable those skilled in the art to implement or use the present invention.To this
A variety of modifications of a little embodiments will be readily apparent to those skilled in the art, as defined herein general
Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention will not
It can be intended to be limited to the embodiments shown herein, and be to fit to consistent with the principles and novel features disclosed in this article
Widest scope.
The test material that the present invention uses is all common commercially available product, can all be bought in market.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1
(1) vitamin C dissolved in purified water is taken, it is 0.5mg/mL vitamin aqueous solution that concentration, which is made,.
(2) glutinous rehmannia fresh rhizome is taken, is cleaned, is removed putrid and deteriorated part, cut 4-6mm sheet, 40 DEG C of forced air dryings 1 are small
When.
(3) according to every 100kg fresh medicine, 1L said vitamin C aqueous solution is sprayed, it is dry under the conditions of 40 DEG C of air blast, until containing
Water is lower than 13%, obtains radix rehmanniae.
Embodiment 2
(1) vitamin C dissolved in purified water is taken, it is 2.0mg/mL vitamin C aqueous solution that concentration, which is made,.
(2) glutinous rehmannia fresh rhizome is taken, is cleaned, is removed putrid and deteriorated part, cut 4-6mm sheet, 40 DEG C of forced air dryings 1 are small
When；
(3) according to every 100kg fresh medicine, 1L said vitamin C aqueous solution is sprayed, it is dry under the conditions of 40 DEG C of air blast, until containing
Water is lower than 13%, obtains radix rehmanniae.
Embodiment 3
(1) vitamin C dissolved in purified water is taken, it is 1.0mg/mL vitamin C aqueous solution that concentration, which is made,.
(2) glutinous rehmannia fresh rhizome is taken, is cleaned, is removed putrid and deteriorated part, cut 4-6mm sheet, 40 DEG C of forced air dryings 1 are small
When；
(3) according to every 100kg fresh medicine, 0.5L said vitamin C aqueous solution is sprayed, it is dry under the conditions of 40 DEG C of air blast, until
Water content is lower than 13%, obtains radix rehmanniae.
Embodiment 4
(1) vitamin C dissolved in purified water is taken, it is 1.0mg/mL vitamin C aqueous solution that concentration, which is made,.
(2) glutinous rehmannia fresh rhizome is taken, is cleaned, is removed putrid and deteriorated part, cut 4-6mm sheet, 40 DEG C of forced air dryings 1 are small
When；
(3) according to every 100kg fresh medicine, 2.0L said vitamin C aqueous solution is sprayed, it is dry under the conditions of 40 DEG C of air blast, until
Water content is lower than 13%, obtains radix rehmanniae.
Embodiment 5
(1) vitamin C dissolved in purified water is taken, it is 1.0mg/mL vitamin C aqueous solution that concentration, which is made,.
(2) glutinous rehmannia fresh rhizome is taken, is cleaned, is removed putrid and deteriorated part, cut 4-6mm sheet, 40 DEG C of forced air dryings 1 are small
When；
(3) according to every 100kg fresh medicine, 1.0L said vitamin C aqueous solution is sprayed, it is dry under the conditions of 40 DEG C of air blast, until
Water content is lower than 13%, obtains radix rehmanniae.
Embodiment 6
(1) vitamin C dissolved in purified water is taken, it is 1.0mg/mL vitamin C aqueous solution that concentration, which is made,.
(2) glutinous rehmannia fresh rhizome is taken, is cleaned, is removed putrid and deteriorated part, cut 6-8mm sheet, 40 DEG C of forced air dryings 1 are small
When；
(3) according to every 100kg fresh medicine, 1L said vitamin C aqueous solution is sprayed, it is dry under the conditions of 40 DEG C of air blast, until containing
Water is lower than 13%, obtains radix rehmanniae.
Embodiment 7
(1) vitamin C dissolved in purified water is taken, it is 1.0mg/mL vitamin C aqueous solution that concentration, which is made,.
(2) glutinous rehmannia fresh rhizome is taken, is cleaned, is removed putrid and deteriorated part, cut 4-6mm sheet, 40 DEG C of forced air dryings 0.5 are small
When；
(3) according to every 100kg fresh medicine, 1.0L said vitamin C aqueous solution is sprayed, it is dry under the conditions of 40 DEG C of air blast, until
Water content is lower than 13%, obtains radix rehmanniae.
Embodiment 8
(1) vitamin C dissolved in purified water is taken, it is 1.0mg/mL vitamin C aqueous solution that concentration, which is made,.
(2) glutinous rehmannia fresh rhizome is taken, is cleaned, is removed putrid and deteriorated part, cut 4-6mm sheet, 40 DEG C of forced air dryings 2 are small
When；
(3) according to every 100kg fresh medicine, 1.0L said vitamin C aqueous solution is sprayed, it is dry under the conditions of 40 DEG C of air blast, until
Water content is lower than 13%, obtains radix rehmanniae.
9 Catalpol of embodiment and acteoside assay
1. instrument and material.
1.1 instruments: LC-20AB liquid chromatograph (LC-20AB pump, CTO-20A column oven, SIL-20A autosampler,
CBM-20A data converter, LC-Solution Data Processing in Chromatography Workstation), Japanese Shimadzu Corporation；LYO-1 vacuum freeze drying
Machine, Dongfulong Sci-Tech Co., Ltd., Shanghai；Ten a ten thousandth assay balance of METTLERAE240 type, Switzerland METTLER；
FA1004B electronic balance, Shanghai Precision Scientific Apparatus Co., Ltd；KQ-250DB type numerical control ultrasonic cleaner, city of Kunshan are super
Sound Instrument Ltd.；X/WT digital display adjusting apparatus electric-heated thermostatic water bath, Yuyao City Xian Xing Instrument Ltd.；Electric heating constant temperature is dry
Dry case, Shanghai City leap one factory of medical instrument, micropipettor match silent winged generation that (Shanghai) Instrument Ltd.；
Multiskan-MK3 type microplate reader matches Mo Feishier Instrument Ltd.；Turbine mixer, Qingpu Shanghai Hu Xi instrument plant.
1.2 reagents: glutinous rehmannia medicinal material, the supply of the Henan place of production are taught through Liaoning University of TCM Chinese traditional medicine identification teaching and research room monarch Zhai Yan
Award the fresh root tuber for being accredited as scrophulariaceae rehmannia glutinosa plant Rehmannia glutinosa Libosch.；Acteoside control
Product, lot number wkq16062004 are purchased from Wei Keqi Biotechnology Co., Ltd, Sichuan Province, are all larger than through HPLC measurement purity
98%；Catalpol reference substance, lot number MUST-15051225 are purchased from Chengdu Man Site Biotechnology Co., Ltd, measure through HPLC pure
Degree is greater than 99.45%；Big mIL6 (IL-6), interleukin 8 (IL-8), Na+-K+ATP enzyme, Ca2+-Mg2+ATP enzyme, lactic acid
Dehydrogenase (SDH), succinate dehydrogenase (LDH) detection kit (being purchased from Shanghai Yu Chun Biotechnology Co., Ltd)；Acetonitrile,
Methanol is chromatographically pure, and water is distilled water, and other reagents are that analysis is pure.
2. active constituent content measuring
2.1 Catalpol measuring method: using high performance liquid chromatography.
Chromatographic condition: chromatographic column: Microsorb C18 (4.6mm × 250mm, 5 μm), column temperature: 30 DEG C, flow velocity:
1.0mL·min-1, PDA detector, Detection wavelength: 203nm.Mobile phase: 0.1% phosphoric acid water (A)-acetonitrile (B), gradient elution,
Elution program: 0-5min (2-3%B)；5-20min (3-4%B)；20-25min (4-4%B)；25-30min (4-2%B)；30-
40min (2-2%B).Solution preparation: (1) preparation of reference substance solution: taking Catalpol, martynoside A appropriate, accurately weighed, sets respectively
In 50mL measuring bottle, adds methanol to dissolve and be settled to scale, every milliliter of reference substance solution containing 0.464mg, 0.155mg is made.
(2) preparation of test solution: precision weighs fresh rehmannia root and (calculates fresh goods amount by coefficient of giving money as a gift, and be homogenized respectively
Prepare test liquid) 6.0g, 5 radix rehmanniae of embodiment, freeze-drying rehmannia tablets, dry radix rehmanniae recen (i.e. Radix Rehmanniae bloom) each 2.0g set tool
It fills in conical flask, methanol 50mL is added in precision, and weighed weight ultrasonic extraction 0.5 hour, is let cool, then weighed weight, is mended with methanol
The weight of sufficient less loss, shakes up, and filtration, precision measures subsequent filtrate 10mL, is concentrated into and closely does, residue is dissolved in water, and is transferred to 10mL
In volumetric flask, and be diluted with water to graduation mark, shake up, with 0.45 μm of filtering with microporous membrane, take subsequent filtrate to get.
Wherein, be lyophilized rehmannia tablets the preparation method comprises the following steps: fresh rehmannia root is cleaned, and crosscutting is 4-5mm sheet, setting ginseng in freeze dryer
Number is 30 DEG C pre-freeze time 4h, lyophilization time 8h, parsing-desiccation temperature and time/6h.
2.2 acteoside measuring methods: high performance liquid chromatography is used.
Chromatographic condition: chromatographic column: Microsorb C18 (4.6mm × 250mm, 5 μm), column temperature: 30 DEG C, flow velocity:
0.8mL·min-1, PDA detector, Detection wavelength: 334nm.Mobile phase: 0.1% phosphoric acid water (A)-acetonitrile (B), gradient elution,
Elution program: 0-10min (20-30%B)；10-30min (30-50%B)；30-31min (50-20%B)；31-45min(20-
20%B), sampling volume is 10 μ L.
Solution preparation: (1) preparation of reference substance solution: taking acteoside reference substance appropriate, accurately weighed, sets 25mL amount
In bottle, adds methanol to dissolve and be settled to scale, every milliliter of reference substance solution containing 0.203mg is made.
(2) preparation of test solution: fresh rehmannia root (calculating fresh goods amount by coefficient of giving money as a gift, and homogenate prepares test liquid) is about
6.0g (is approximately equivalent to dry product 2.0g), 5 radix rehmanniae of the embodiment of the present invention and radix rehmanniae recen powder each about 2.0g, accurately weighed, sets
In stuffed conical flask, methanol 50mL is added in precision, and weighed weight ultrasonic extraction 0.5 hour, is let cool, then weighed weight, uses methanol
The weight for supplying less loss, shakes up, and filtration, precision measures subsequent filtrate 10mL, is concentrated into and closely does, residue is dissolved in water, and is transferred to
In 10mL volumetric flask, and be diluted with water to graduation mark, shake up, with 0.45 μm of filtering with microporous membrane, take subsequent filtrate to get.
2.3 Fig. 1~4 are the analysis chart under 2.1 chromatographic conditions, and wherein Fig. 1 is the chromatogram of Catalpol standard items, and Fig. 2 is fresh
The chromatogram of glutinous rehmannia sample, Fig. 3 are the chromatogram of 5 radix rehmanniae of the embodiment of the present invention；Fig. 4 is the chromatogram of dry radix rehmanniae recen；
Fig. 5~8 are the analysis chart under 2.2 chromatographic conditions, and wherein Fig. 5 is the chromatogram of acteoside standard items, and Fig. 6 is fresh rehmannia root sample
The chromatogram of product, Fig. 7 are the chromatogram of 5 radix rehmanniae of the embodiment of the present invention；Fig. 8 is the chromatogram of dry radix rehmanniae recen.Effectively at
Assay is divided to the results are shown in Table 1.
The content (n=3) of Catalpol and acteoside in 1 different processing methods Rehmannia glutinosa of table
Embodiment 10
The grouping of 3.1 heat-clearing experimental animals and modeling
After SD male rat adaptive feeding 7d, blank control group, model group (yeast), model group are randomly divided by weight
(LPS), positive drug group (yeast), positive drug group (LPS), fresh rehmannia root juice group (yeast), fresh rehmannia root juice group (LPS), 5 groups of embodiment
5 groups of (yeast), embodiment (LPS), radix rehmanniae recen group (yeast), radix rehmanniae recen group (LPS).In formally experiment the last week, three times a day
It is early, middle and late each once the rectum of rat to be stimulated.It when formal experiment, is measured rat temperature 3 times in 0.5 hour, takes it flat
Mean value is as basic body temperature value.Each group gastric infusion: blank control group and model group press 10ml/kg stomach-filling physiological saline；It is positive
Control group press 0.2g/kg stomach-filling aspirin, each administration group according to quantity 10 times of gastric infusions.0.5h starts after administration
Modeling.
(1) yeast causes rat fever model: dry ferment is made into physiological saline 20% suspension by experiment before starting, removed
Outside blank group, other each groups are administered to SD rat by the dose subcutaneous of 10ml/kg, after dry ferment suspension is subcutaneously injected, per small
When survey body temperature 1 time (monitoring 8 hours).
(2) LPS lipopolysaccharides causes rat fever model: before experiment starts, LPS being made into the storage of 10 μ g/ml with physiological saline
Standby liquid, in addition to blank group, other each groups are injected intraperitoneally by 20 μ g/kg dosage and give SD rat, after LPS is injected intraperitoneally, are surveyed per hour
Body temperature is primary (monitoring 7 hours).
3.2 Indexs measure
3.2.1 sample collection: after the last administration, being deprived of food but not water for 24 hours, next day weighed rat body weight, then with 10% second
Ehterization, abdominal aortic blood are placed at room temperature for 0.5h, and 3000r/min is centrifuged 10min, draws supernatant, save in -20 DEG C, to
It surveys.
3.2.2 the measurement of serum sample: the serum sample of freezen protective is put to room temperature, is said according to ELISA kit
Bright book is operated, and interleukin 6 (IL-6), interleukin 8 (IL-8), Na+-K+-ATP enzyme, Ca2+-Mg2 in rat blood serum are measured
The content of+- ATP enzyme, lactic dehydrogenase (SDH), succinate dehydrogenase (LDH).
3.3 statistical analysis
Statistical procedures are carried out using 17.0 software of SPSS, measurement data is indicated with X ± S, and each group of data carries out normal state point
Cloth is examined and homogeneity test of variance.Examined using variance analysis and t and carry out comparison among groups, P < 0.05 be it is variant, there is statistics
Learn meaning.
3.4 result
Na in each group rat blood serum after being administered with ELISA method detection+-K+ATP enzyme, Ca2+-Mg2+ATP enzyme, SDH, LDH
Content the results are shown in Table 2,3.Interleukin 6 (IL-6), interleukin 8 (IL-8) in each group rat blood serum after being administered with ELISA method detection
Content, the results are shown in Table 4,5.
Table 2 causes Na in fever model rat blood serum to yeast+-K+ATP enzyme, Ca2+-Mg2+ATP enzyme, SDH,
The influence (x ± S, n=8) of LDH content
Note: *: P < 0.05 compared with model group；#: compared with blank group, P < 0.05；▲: compared with Radix Rehmanniae: P < 0.05
Table 3 causes Na in fever model rat blood serum to LPS+-K+ATP enzyme, Ca2+-Mg2+ATP enzyme, SDH,
LDH content influence (N=8)
Note: *: P < 0.05 compared with model group；#: compared with blank group, P < 0.05；▲: compared with Radix Rehmanniae: P < 0.05
The results show that model group is compared with blank group, Na in serum+-K+ATP enzyme, Ca2+-Mg2+ATP enzyme, SDH, LDH
Content it is significantly raised, difference has statistical significance (P < 0.05), shows modeling success；Compare between each administration group and model group
More also there is difference (P < 0.05), shows that drug has therapeutic effect.Wherein, fresh rehmannia root juice group is with 5 groups of embodiment in Na+-K+-
ATP enzyme, Ca2+-Mg2+ATP enzyme, the upper no significant difference of SDH, LDH, but there is significant difference between radix rehmanniae recen medicine materical crude slice,
Show that fresh rehmannia root juice and radix rehmanniae of the present invention are close in the effect for adjusting energetic supersession, is significantly better than radix rehmanniae recen (P <
0.05).Table 4 to yeast cause fever model rat blood serum in IL-6, IL-8 content influence (N=8)
Note: *: P < 0.05 compared with model group；#: compared with blank group, P < 0.05；▲: compared with Radix Rehmanniae: P < 0.05
5 glutinous rehmannia raw product of table to yeast cause fever model rat blood serum in IL-6, IL-8 content influence (N=8)
Note: *: P < 0.05 compared with model group；#: compared with blank group, P < 0.05；▲: compared with Radix Rehmanniae: P < 0.05
4~table of table 5 the results show that model group compared with blank group, interleukin 6 (IL-6), interleukin 8 (IL-8) in serum
Content is significantly raised, and difference has statistical significance (P < 0.05), shows modeling success；Each administration group and model comparison among groups
Also there is difference (P < 0.05), shows that each group drug has therapeutic effect, fresh rehmannia root juice and radix rehmanniae of the present invention are in interleukin 6
(IL-6), no significant difference on interleukin 8 (IL-8), but there is significant difference between the two and radix rehmanniae recen medicine materical crude slice, show
Fresh rehmannia root juice and glutinous rehmannia primary colors medicine materical crude slice are better than radix rehmanniae recen in the effect for adjusting energetic supersession, and the two effect is approximate.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of radix rehmanniae, which is characterized in that its catalpol content >=79.35mgg-1。
2. radix rehmanniae according to claim 1, which is characterized in that its acteoside content >=7.11mgg-1。
3. a kind of preparation method of radix rehmanniae, includes the following steps:
Step 1: glutinous rehmannia fresh rhizome being taken to be sliced；
Step 2: by slice made from step 1, uniformly spray vitamin C aqueous solution, forced air drying to water content are lower than 13%, obtain
Radix rehmanniae.
4. preparation method according to claim 1, which is characterized in that in step 1, the slice thickness is 2-8mm.
5. preparation method according to claim 1, which is characterized in that further include that air blast is dry after the slice in step 1
Dry step, the time of the forced air drying are 0.5~2 hour, and the temperature of forced air drying is 40 DEG C.
6. preparation method according to claim 1, which is characterized in that in step 2, the concentration of the vitamin C aqueous solution is
0.5-2mg/mL。
7. preparation method according to claim 1, which is characterized in that in step 2, the spray is according to every 100kg glutinous rehmannia
Fresh rhizome is sprayed with 0.5-2L vitamin C aqueous solution.
8. preparation method according to claim 1, which is characterized in that in step 2, the temperature of the forced air drying is 40 DEG C.
9. radix rehmanniae made from any one of claim 3~8 preparation method.
10. application of the radix rehmanniae in the drug of preparation treatment fever due to yin deficiency according to claim 9.
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