CN105145957B - A kind of health-care herbal tea beverage improving oral ulcer and preparation method thereof and detection method - Google Patents
A kind of health-care herbal tea beverage improving oral ulcer and preparation method thereof and detection method Download PDFInfo
- Publication number
- CN105145957B CN105145957B CN201510643609.5A CN201510643609A CN105145957B CN 105145957 B CN105145957 B CN 105145957B CN 201510643609 A CN201510643609 A CN 201510643609A CN 105145957 B CN105145957 B CN 105145957B
- Authority
- CN
- China
- Prior art keywords
- parts
- health
- solution
- methanol
- herba
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to food processing technology field, be specifically related to a kind of health-care herbal tea beverage treating oral ulcer and preparation method thereof and detection method.This health beverage is health tea, is made up of the raw material of following weight parts: green tea 5～10 parts, Herba Cymbopogonis Citrari 1～5 parts, and Mei Qiangwie spends 1～5 part, Herba Biondiae henryi 1～5 parts, Flos Rhoeo discolorae 1～5 parts, Radix epipactinis 1～5 parts.Its preparation method is: take Herba Cymbopogonis Citrari, Herba Biondiae henryi, Radix epipactinis boiling 3 times, each 1 hour, and collecting decoction filters, and filtrate is concentrated into the thick paste that suitable density is 1.25；Take Mei Qiangwie flower, Flos Rhoeo discolorae is ground into coarse powder, mixes with thick paste, is dried, and adds green tea, mixing, sieves, and loads in teabag filter paper bag, sealing.
Description
Technical field
The invention belongs to food processing technology field, relate to a kind of health beverage, being specially one has one to oral ulcer
Determine the health tea of therapeutical effect.
Background technology
Recurrent oral ulceration is also known as recurrent aphtha (recurrentaphthous ulcer, RAU), recurrence
Property oral ulcer, is modal oral mucosal disease, and its sickness rate is up to about 20%.The lighter's several months recurs once, and severe one is slow
Property delay, recurrent exerbation, hinder patient diet or speak, make it feel the most painful.The recurrent aphtha cause of disease cause of disease is the most not clear
Really, and there is obvious individual variation, the most clinically without effective Therapeutic Method. the most many scholar's morbidities to RAU
Mechanism has carried out numerous studies, it is believed that recurrent oral ulceration is the ulcer disease that immunologic dysfunction causes, and wherein oxygen is certainly
May be played an important role in the generation evolution of RAU by base damage, microcirculation disturbance and trace element deficiency.
Although modern medicine there has been deeper understanding to the pathogenesis of RAU, but owing to its cause of disease still can not be the brightest
Really, therefore clinic there is no preferable Therapeutic Method at present, and some its effects of traditional Therapeutic Method are the most unsatisfactory.In recent years
Coming, inventor uses health tea of the present invention for treating RAU, achieves preferable curative effect.
In the present invention:
Herba Cymbopogonis Citrari is grass family Cymbopogon plants Herba Cymbopogonis CitrariCymbopogon citratus (DC.)StapfFlower.
Mei Qiangwie flower is Rosaceae gul Mei QiangwieRosa bella Rehd. et WilsFlower.
Herba Biondiae henryi is trailing plants section biondia hennriyi platymiscium biondia hennriyiBiondia henryi(Warb. ex Schltr. et Diels)Tsiang et P.T.Li[Cynanchum henryi Warb. ex Schltr. et Diels]Whole herb with root.
Flos Rhoeo discolorae is Commelianaceae Rhoeo plant purple Rohdea japonica RothRhoeo discolor (L ´ Herit.)Hance
Flower.
Radix epipactinis is the orchid family helleborine platymiscium little Hua helleborineEpipactis helleborine(L.) Crantz [Serapias helleborine L.;S. latifolia (L.)All.]Root.
Summary of the invention
It is an object of the invention to provide a kind of health beverage with treatment oral ulcer effect, this health beverage is health care
Tea.
It is a further object of the present invention to provide the preparation method of this health tea.
It is an object of the invention to be accomplished by:
A kind of health beverage with treatment oral ulcer effect, this health beverage is health tea, is by following weight parts
Raw material make: green tea 5～10 parts, Herba Cymbopogonis Citrari 1～5 parts, Mei Qiangwie spends 1～5 part, Herba Biondiae henryi 1～5 parts, Flos Rhoeo discolorae 1～5 parts,
Radix epipactinis 1～5 parts.
Described health beverage is made up of the raw material of following weight parts: green tea 10 parts, 5 parts of Herba Cymbopogonis Citrari, and Mei Qiangwie spends 5 parts,
Herba Biondiae henryi 5 parts, Flos Rhoeo discolorae 4 parts, Radix epipactinis 4 parts.
Described health beverage can also be made up of the raw material of following weight parts: green tea 10 parts, 2 parts of Herba Cymbopogonis Citrari, Mei Qiangwie
Spend 3 parts, Herba Biondiae henryi 4 parts, Flos Rhoeo discolorae 5 parts, Radix epipactinis 4 parts.
Above-mentioned health beverage is made up of following method: take Herba Cymbopogonis Citrari, Mei Qiangwie flower, Herba Biondiae henryi, Flos Rhoeo discolorae, Radix epipactinis, roasting
Dry, pulverize, mix with green tea, use conventional method to make bulk tea, compressed tea.
Above-mentioned health beverage can also be made up of following method: takes Herba Cymbopogonis Citrari, Mei Qiangwie flower, Herba Biondiae henryi, Flos Rhoeo discolorae, wild bamboo
Orchid, dries, and pulverizes, and mixes with green tea, uses conventional method to make bulk tea, compressed tea, teabag.
Above-mentioned health beverage can also be made up of following method: takes Herba Cymbopogonis Citrari, Herba Biondiae henryi, Radix epipactinis boiling 3 times, often
Secondary 1 hour, collecting decoction, filter, filtrate is concentrated into the thick paste that suitable density is 1.25；Take Mei Qiangwie flower, Flos Rhoeo discolorae is ground into
Coarse powder, mixes with thick paste, is dried, and adds green tea, mixing, sieves, and loads in teabag filter paper bag, sealing, every bag of weight 2g.
Health beverage of the present invention can use following method to carry out quality testing:
Employing high effective liquid chromatography for measuring verbascoside (verbascoside) content:
(1) chromatographic condition: chromatographic column is C18Chromatographic column；With the methanol-0.2mol/L phosphoric acid that ratio is 40～60:40～60
Sodium dihydrogen solution is flowing phase；Detection wavelength 300～330nm, column temperature 25～45 DEG C；
(2) preparation of reference substance solution: precision weighs verbascoside reference substance and is placed in volumetric flask, adds methanol and dissolves and dilute
Release to scale, shake up；Pipette above-mentioned solution and put volumetric flask, add methanol constant volume, as reference substance stock solution；
(3) preparation of need testing solution: take health tea of the present invention, precision weighing, puts in tool plug conical flask, accurate addition first
Alcohol, close plug, weighed weight, supersound process, let cool, more weighed weight, supply the weight of less loss with methanol, shake up, microporous filter membrane
Filter, take subsequent filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, reference substance solution each 5～20 μ L respectively, inject high-efficient liquid phase color
Spectrometer, detects.
Health beverage of the present invention preferably employs following method and carries out quality testing:
Employing high effective liquid chromatography for measuring verbascoside content:
(1) chromatographic condition: chromatographic column is C18Chromatographic column；Methanol-0.2mol/L sodium dihydrogen phosphate with ratio as 40:60 is molten
Liquid is flowing phase；Detection wavelength 312nm, column temperature 35 DEG C；Number of theoretical plate is calculated by verbascoside peak should be not less than 4000；
(2) preparation of reference substance solution: precision weighs verbascoside reference substance 10.00mg and is placed in 100ml volumetric flask, adds
Methanol dissolves and is diluted to scale, shakes up；Pipette above-mentioned solution 10ml and put 25ml volumetric flask, add methanol constant volume, as reference substance
Stock solution；
(3) preparation of need testing solution: take health tea 3g of the present invention, precision weighing, puts in tool plug conical flask, accurate addition
Methanol 20ml, close plug, weighed weight, supersound process 30min, let cool, more weighed weight, supply the weight of less loss with methanol, shake
Even, 0.45 μm filtering with microporous membrane, take subsequent filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatography
Instrument, detects.
This health beverage is for preparing the purposes in treatment oral ulcer, gingivitis, periodontitis health beverage.
Experiment one: the effect observation of health tea of the present invention treatment oral ulcer
1 general clinical data
1.1 ordinary circumstances:
In primary disease 60 example outpatient, male 30 example, women 30 example；
Age of onset 30～50 years old, 30 examples；50～60 years old, 18 examples；60～70 years old, 12 examples；
Course of disease January～March, 29 examples；April～May, 21 examples；More than half a year, 10 examples.
60 example patients are randomly divided into conventional group and observation group:
Observation group:
Totally 30 example, male 15 example, women 15 example.
Age of onset 30～50 years old, 15 examples；50～60 years old, 10 examples；60～70 years old, 5 examples.
Course of disease January～March, 14 examples；April～May, 10 examples；More than half a year, 6 examples.
Conventional group:
Totally 30 example, male 15 example, women 15 example.
Age of onset 30～50 years old, 15 examples；50～60 years old, 8 examples；60～70 years old, 7 examples.
Course of disease January～March, 15 examples；April～May, 11 examples；More than half a year, 4 examples.
Two groups of patients in the treatment of age, sex and other drug without significant difference.
1.2 case selections: oral mucosa occur single or multiple diameters in the circle of 3～5 millimeter or bigger, ellipse
The circular ulcer shallow compared with table, skin surface has secretions to adhere to. and the most congested, with scorching hot, pain, inconvenience of speaking, enter
Food difficulty, sialorrhea, General Symptoms even occurs.Ulcer recurrent exerbation, the little person of therapeutic effect.Get rid of behcets disease, carcinous or
Tuberculous ulcer.
1.3 Therapeutic Method
The patient of observation group, uses health tea of the present invention to be taken after being infused in hot water or decoction frequently and drinks.5 days 1 courses for the treatment of, general 1～2 course for the treatment of is i.e.
Stop, disable any other medicines during this period.
The patient of conventional group, uses green tea to be taken after being infused in hot water or decoction frequently and drinks.5 days 1 courses for the treatment of, general 1～2 course for the treatment of i.e. stopped, in this phase
Between disable any other medicines.
1.4 oral ulcer diagnostic criterias
Curing: clinical symptom disappearance, oral ulcer heals, and has no recidivist in following up a case by regular visits to 1 year；
Effective: clinical symptom relief, oral ulcer heals substantially；
Take a turn for the better: ulcer recurrence time lengthening after treatment, the state of an illness alleviates, and ulcer significantly reduces；
Invalid: after drug withdrawal, ulcer has recurred.
1.5 statistical method
Employing SPSS13.0 statistical software, continuous data use (± s) represent, using t inspection, enumeration data uses χ2Inspection
Test, inspection level α=0.05.
1.6 therapeutic outcomes:
Therapeutic outcome is shown in Table 1-1.
Oral ulcer comparitive study after table 1-1 treatment
Note: observation group vs routine group, P ＜ 0.05.
Drink health tea of the present invention, in 3 days, cure 17 examples, cure 12 in 7～10 days and fall.Typically take effect at 2～5 days.
6 conclusions
Health tea tea energy enhancing human body immunity power of the present invention, to staphylococcus aureus, escherichia coli, streptococcus pneumoniae, second
Type streptococcus all has good inhibiting effect.The pH value of health tea of the present invention is 6～7, of substantially equal with the pH value in oral cavity, drinks
Health tea of the present invention is conducive to the growth of Oral normal flora, has good Nutrition to wound surface, and persistently using can be obvious
Treatment oral ulcer, increases mouth comfort.Therefore, health tea of the present invention is a kind of economic, effective, the peace for the treatment of oral ulcer
Full health promoting beverage.
2 model cases
Zhang, male, 35 years old, certain enterprise office civilian, first visit on June 4 in 2015.Recurrent exerbation oral ulcer 2
For many years, tongue and oral mucosa having multiple ulcer differed in size, attaches most importance to left side, hurt like hell, eating difficulties, speech is not
Just, dizzy weak, emaciated physique, feverish sensation in the palms and soles, uneasy sleeping at night.1 wheat harvesting period is gone through in this time outbreak, and patient is the most painful, in warp
Doctor trained in Western medicine multi-treatment, poor effect, come to go to a doctor upon somebody's introduction, red tongue, the micro-Huang of thin fur, thready and stringy pulse.Distinguish as yin asthenia generating intrinsic heat, control
With clearing away heat and nourishing YIN, i.e. giving health tea of the present invention and be taken after being infused in hot water or decoction, join 5 bags altogether, wrap every day 1, advise frequency drink, 4 bags that are only taken after being infused in hot water or decoction i.e. are fully recovered, so far
Have no recurrence, good results.
Experiment two: the quality standard research of health tea of the present invention
Use verbascoside content in high effective liquid chromatography for measuring health tea of the present invention
The preparation of 1 sample solution
The preparation of 1.1 reference substance solution
Precision weighs verbascoside reference substance 10.00mg and is placed in 100ml volumetric flask, adds methanol and dissolves and be diluted to carve
Degree, shakes up.Pipette above-mentioned solution 10ml and put 25ml volumetric flask, add methanol constant volume, as reference substance stock solution (verbascoside
40.80 μ g/ml).
The preparation of 1.2 need testing solutions
Prescription: green tea 30 kilograms, 15 kilograms of Herba Cymbopogonis Citrari, Mei Qiangwie spends 15 kilograms, Herba Biondiae henryi 15 kilograms, Flos Rhoeo discolorae 12,000
Gram, Radix epipactinis 12 kilograms；
Preparation method: take Herba Cymbopogonis Citrari, Herba Biondiae henryi, Radix epipactinis boiling 3 times, each 1 hour, collecting decoction, filter, filtrate is dense
It is reduced to the thick paste that suitable density is 1.25；Take Mei Qiangwie flower, Flos Rhoeo discolorae is ground into coarse powder, mixes with thick paste, is dried, adds green
Tea, mixing, sieve, use conventional method to make teabag, every pouch 2 grams.
Take health tea 3g of the present invention, precision weighing, put in tool plug conical flask, the accurate methanol 20ml that adds, close plug is weighed heavy
Amount, supersound process 30min, let cool, more weighed weight, supply the weight of less loss with methanol, shake up, 0.45 μm microporous filter membrane mistake
Filter, takes subsequent filtrate, obtains need testing solution.
The preparation of 1.3 negative controls
Remaining composition taken in prescription composition in addition to Herba Cymbopogonis Citrari makes the negative controls without Herba Cymbopogonis Citrari, makes by " 1.2 "
Obtain negative control solution.Go out peak position at verbascoside and absworption peak does not occurs, other compositions pair in health tea of the present invention are described
The assay of verbascoside does not interferes with.
2 chromatographic conditions
Chromatographic column is SHIMADZU C18Chromatographic column (5 μm, 4.6mm × 150.0mm, Shimadzu Corporation)；With methanol-0.2mol/
L sodium dihydrogen phosphate (40:60) is flowing phase；Detection wavelength 312nm, column temperature 35 DEG C.Number of theoretical plate is based on verbascoside peak
Calculation should be not less than 4000；With this understanding, in health tea of the present invention, verbascoside separates good.
3 methodological studies.
3.1 the range of linearity
Accurate draw above-mentioned verbascoside reference substance solution, add methanol dilution become verbascoside concentration to be respectively 0.816,
1.632, the solution of 3.264,4.896,6.528,8.160 μ g/ml, successively sample introduction 10 μ l.With peak area (Y) as vertical coordinate, with
Sample introduction concentration (X) is that abscissa carries out linear regression, obtains regression equation:
Y=3.638 × 104X 1.068 × 104, correlation coefficient r=0.9996.Show verbascoside concentration 0.816～
It is good linear relation with chromatographic peak area in the range of 8.160 μ g/ml.
3.2 precision test
Taking the verbascoside reference substance solution 10 μ l METHOD FOR CONTINUOUS DETERMINATION 5 times that concentration is 4.890 μ g/ml, RSD is 0.15%, shows
Instrument precision is good.
3.3 stability test
Take same need testing solution 0,2,4,6,8h sample introduction 10 μ l respectively, record peak area, result shows: test sample is molten
Liquid is stable in room temperature places 8h, and verbascoside peak area RSD is 1.60%.
3.4 replica test
By health tea of the present invention, parallel extraction prepares 6 parts of need testing solutions, and sample introduction measures respectively, and RSD is 1.23%(n=
6), show that the method repeatability is good.
3.5 average recovery tests
Precision weighs each 9 parts of the health tea of the present invention of known content, adds the verbascoside reference substance of respective amount, by for examination
The preparation method of product solution processes and measures.Average recovery result of the test is shown in Table 2-1.Verbascoside average recovery rate is
97.7%, RSD are 1.66%, show that the method is accurately, reliably.
Table 2-1 recovery test result (n=9)
3.6 sample sizes measure.Take 4 batches, health tea sample of the present invention, the method prepared according to need testing solution, 0.45 μm
Sample introduction after filtering with microporous membrane, different lot number health tea of the present invention content are shown in Table 2-2.
Table 2-2 sample determination result (n=3)
Detailed description of the invention:
Embodiment 1:
Prescription: green tea 10 kilograms, 5 kilograms of Herba Cymbopogonis Citrari, Mei Qiangwie spends 5 kilograms, Herba Biondiae henryi 5 kilograms, Flos Rhoeo discolorae 4 kilograms, wild
Blue 4 kilograms of bamboo.
Take above-mentioned Herba Cymbopogonis Citrari, Mei Qiangwie flower, Herba Biondiae henryi, Flos Rhoeo discolorae, Radix epipactinis, dry, be crushed to 20 mesh, mix with green tea
Even, use conventional method to make teabag, every pouch 2 grams.
Embodiment 2:
Prescription: green tea 10 kilograms, 2 kilograms of Herba Cymbopogonis Citrari, Mei Qiangwie spends 3 kilograms, Herba Biondiae henryi 4 kilograms, Flos Rhoeo discolorae 5 kilograms, wild
Blue 4 kilograms of bamboo.
Preparation method: take above-mentioned Herba Cymbopogonis Citrari, Mei Qiangwie flower, Herba Biondiae henryi, Flos Rhoeo discolorae, Radix epipactinis, dry, pulverizes, and mixes with green tea,
Conventional method is used to make compressed tea.
Embodiment 3:
Prescription: green tea 5 kilograms, 4 kilograms of Herba Cymbopogonis Citrari, Mei Qiangwie spends 5 kilograms, Herba Biondiae henryi 5 kilograms, Flos Rhoeo discolorae 1 kilogram, wild bamboo
Blue 5 kilograms.
Preparation method: take above-mentioned Herba Cymbopogonis Citrari, Mei Qiangwie flower, Herba Biondiae henryi, Flos Rhoeo discolorae, Radix epipactinis, dry, pulverizes, and mixes with green tea,
Conventional method is used to make bulk tea.
Embodiment 4:
Prescription: green tea 30 kilograms, 15 kilograms of Herba Cymbopogonis Citrari, Mei Qiangwie spends 15 kilograms, Herba Biondiae henryi 15 kilograms, Flos Rhoeo discolorae 12,000
Gram, Radix epipactinis 12 kilograms；
Preparation method: take Herba Cymbopogonis Citrari, Herba Biondiae henryi, Radix epipactinis boiling 3 times, each 1 hour, collecting decoction, filter, filtrate is dense
It is reduced to the thick paste that suitable density is 1.25；Take Mei Qiangwie flower, Flos Rhoeo discolorae is ground into coarse powder, mixes with thick paste, is dried, adds green
Tea, mixing, sieve, use conventional method to make teabag, every pouch 2 grams.
Detection method: employing high effective liquid chromatography for measuring verbascoside content:
(1) chromatographic condition: chromatographic column is SHIMADZU C18Chromatographic column；Methanol-0.2mol/L phosphorus with ratio as 40:60
Acid dihydride sodium solution is flowing phase；Detection wavelength 312nm, column temperature 35 DEG C；Number of theoretical plate is calculated should be not less than by verbascoside peak
4000；
(2) preparation of reference substance solution: precision weighs verbascoside reference substance 10.00mg and is placed in 100ml volumetric flask, adds
Methanol dissolves and is diluted to scale, shakes up；Pipette above-mentioned solution 10ml and put 25ml volumetric flask, add methanol constant volume, as reference substance
Stock solution；
(3) preparation of need testing solution: take health tea 3g of the present invention, precision weighing, puts in tool plug conical flask, accurate addition
Methanol 20ml, close plug, weighed weight, supersound process 30min, let cool, more weighed weight, supply the weight of less loss with methanol, shake
Even, 0.45 μm filtering with microporous membrane, take subsequent filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatography
Instrument, detects；
(5) result: in every gram of health tea of the present invention, verbascoside content is 50.73 μ g.
Although moreover, it will be appreciated that this specification is been described by according to embodiment, but the most each embodiment only wraps
Containing an independent technical scheme, this narrating mode of description is only that for clarity sake those skilled in the art should
Description can also be formed those skilled in the art through appropriately combined as an entirety, the technical scheme in each embodiment
May be appreciated other embodiments.
Claims (5)
1. the health beverage improving oral ulcer, it is characterised in that this health beverage is health tea, is by following weight parts
Raw material make: green tea 5～10 parts, Herba Cymbopogonis Citrari 1～5 parts, Mei Qiangwie spends 1～5 part, Herba Biondiae henryi 1～5 parts, Flos Rhoeo discolorae 1～5 parts,
Radix epipactinis 1～5 parts；This health beverage is made up of following method: take Herba Cymbopogonis Citrari, Herba Biondiae henryi, Radix epipactinis boiling 1～4 times, often
Secondary 0.5～2 hour, collecting decoction, filter, filtrate is concentrated into the thick paste that suitable density is 1.10～1.30；Take Mei Qiangwie flower, freshwater mussel
Cymbidium ensifolium (L.) Sw. is ground into coarse powder, mixes with thick paste, is dried, and adds green tea, mixing, sieves, loads in teabag filter paper bag.
2. health beverage as claimed in claim 1, it is characterised in that this health beverage is made up of the raw material of following weight parts: green
Tea 10 parts, 5 parts of Herba Cymbopogonis Citrari, Mei Qiangwie spends 5 parts, Herba Biondiae henryi 5 parts, Flos Rhoeo discolorae 4 parts, Radix epipactinis 4 parts.
3. health beverage as claimed in claim 1, it is characterised in that this health beverage is made up of following method: take Herba Cymbopogonis Citrari,
Herba Biondiae henryi, Radix epipactinis boiling 3 times, each 1 hour, collecting decoction, filter, filtrate be concentrated into suitable density be 1.25 thick
Cream；Take Mei Qiangwie flower, Flos Rhoeo discolorae is ground into coarse powder, mixes with thick paste, is dried, and adds green tea, mixing, sieves, loads teabag
In filter paper bag.
4. the quality determining method of health beverage as claimed in claim 1, it is characterised in that this health beverage uses following method
Carry out quality testing: use high effective liquid chromatography for measuring verbascoside content:
(1) chromatographic condition: chromatographic column is C18Chromatographic column；With the methanol-0.2mol/L biphosphate that ratio is 40～60:40～60
Sodium solution is flowing phase；Detection wavelength 300～330nm, column temperature 25～45 DEG C；
(2) preparation of reference substance solution: precision weighs verbascoside reference substance and is placed in volumetric flask, adds methanol and dissolves and be diluted to
Scale, shakes up；Pipette above-mentioned solution and put volumetric flask, add methanol constant volume, as reference substance stock solution；
(3) preparation of need testing solution: take health tea of the present invention, precision weighing, puts in tool plug conical flask, accurate addition methanol,
Close plug, weighed weight, supersound process, let cool, more weighed weight, supply the weight of less loss with methanol, shake up, microporous filter membrane mistake
Filter, takes subsequent filtrate, obtains need testing solution；
(4) measure: precision measures above-mentioned need testing solution, reference substance solution each 5～20 μ L respectively, inject high performance liquid chromatography
Instrument, detects.
5. the quality determining method of health beverage as claimed in claim 4, it is characterised in that this health beverage uses following method
Carry out quality testing: use high effective liquid chromatography for measuring verbascoside content:
(1) chromatographic condition: chromatographic column is C18Chromatographic column；Methanol-0.2mol/L sodium dihydrogen phosphate with ratio as 40:60 is
Flowing phase；Detection wavelength 312nm, column temperature 35 DEG C；Number of theoretical plate is calculated by verbascoside peak should be not less than 4000；
(2) preparation of reference substance solution: precision weighs verbascoside reference substance 10.00mg and is placed in 100ml volumetric flask, adds methanol
Dissolve and be diluted to scale, shaking up；Pipette above-mentioned solution 10ml and put 25ml volumetric flask, add methanol constant volume, stock as reference substance
Liquid；
(3) preparation of need testing solution: take health tea 3g of the present invention, precision weighing, puts in tool plug conical flask, accurate addition methanol
20ml, close plug, weighed weight, supersound process 30min, let cool, more weighed weight, supply the weight of less loss with methanol, shake up,
0.45 μm filtering with microporous membrane, takes subsequent filtrate, obtains need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, enter
Row detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510643609.5A CN105145957B (en) | 2015-10-08 | 2015-10-08 | A kind of health-care herbal tea beverage improving oral ulcer and preparation method thereof and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510643609.5A CN105145957B (en) | 2015-10-08 | 2015-10-08 | A kind of health-care herbal tea beverage improving oral ulcer and preparation method thereof and detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105145957A CN105145957A (en) | 2015-12-16 |
| CN105145957B true CN105145957B (en) | 2016-10-05 |
Family
ID=54787357
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510643609.5A Active CN105145957B (en) | 2015-10-08 | 2015-10-08 | A kind of health-care herbal tea beverage improving oral ulcer and preparation method thereof and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105145957B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106071506A (en) * | 2016-08-25 | 2016-11-09 | 陈伊芸 | A kind of special antipyretic beverage of oral ulcer and preparation method thereof |
| CN106552097A (en) * | 2016-11-29 | 2017-04-05 | 广西大学 | A kind of health protection tea for treating canker sore |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726558A (en) * | 2011-04-10 | 2012-10-17 | 董爱香 | Health care green tea |
| CN103141631A (en) * | 2013-02-20 | 2013-06-12 | 蓝照斓 | Japanese raspberry root health-care tea |
| CN103520502A (en) * | 2013-10-18 | 2014-01-22 | 李佃场 | Chinese medicinal preparation for treating gingivitis and preparation method thereof |
-
2015
- 2015-10-08 CN CN201510643609.5A patent/CN105145957B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105145957A (en) | 2015-12-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101396466B (en) | Cold medicine tea composition as medicine and food, preparation method and use thereof | |
| WO2015172608A1 (en) | Capsule for assisting in reducing blood fat and preparation method therefor | |
| CN102551065B (en) | Blood sugar reducing food series | |
| CN103919920A (en) | Buccal tablet retaining original ecology of wolfberry fruits and preparation process thereof | |
| CN109007514A (en) | A kind of composition and preparation method thereof with raising immunity function | |
| CN105145957B (en) | A kind of health-care herbal tea beverage improving oral ulcer and preparation method thereof and detection method | |
| CN101856418B (en) | Pharmaceutical preparation for preventing nephritis and preparation method thereof | |
| CN103816278B (en) | Composition for reducing blood sugar and application thereof | |
| CN105106816A (en) | Traditional Chinese medicine healthcare preparation with protection effect on chemical liver injury and preparing method thereof | |
| CN102885307B (en) | Composition for damp-heat constitutions, preparation method and applications thereof | |
| CN106420955A (en) | Traditional Chinese medicine composition with blood glucose reduction assistance effect, preparation method and product of traditional Chinese medicine composition | |
| KR20060102621A (en) | Composition containing sanguisorba officinalis extract for treatment hypersensitive skin disease | |
| CN114794478A (en) | Composition capable of reducing blood pressure, blood fat and blood sugar and application thereof | |
| CN104127816B (en) | A kind of pharmaceutical composition for treating diabetes and its production and use | |
| CN107050143A (en) | It is a kind of that there is the formula for improving sleep effect, composition and preparation method thereof | |
| WO2021063016A1 (en) | Composition with hypolipidemic function, preparation method therefor and application thereof | |
| CN112206283A (en) | Traditional Chinese medicine composition for relieving physical fatigue and preparation method thereof | |
| CN110403166A (en) | A kind of energy food and preparation method thereof | |
| CN110720527A (en) | Traditional Chinese medicine weight-losing tea | |
| CN105166929B (en) | Health drink beneficial to sleep as well as preparation method and detection method of health drink | |
| CN105360787A (en) | Health food and preparation method thereof | |
| CN103719866B (en) | A kind of composition and use thereof, preparation method with effect of weight reducing | |
| CN109430667A (en) | A kind of composition and solid beverage with anti-trioxypurine effect | |
| CN108260816A (en) | A kind of highland barley seedling health food and preparation method thereof | |
| CN108260815A (en) | It is a kind of have the function of reducing blood lipid, weight-reducing health food and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |