CN109452445A - A kind of preparation method of high-purity sea cucumber Gly-His-Lys - Google Patents
A kind of preparation method of high-purity sea cucumber Gly-His-Lys Download PDFInfo
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- CN109452445A CN109452445A CN201811461029.4A CN201811461029A CN109452445A CN 109452445 A CN109452445 A CN 109452445A CN 201811461029 A CN201811461029 A CN 201811461029A CN 109452445 A CN109452445 A CN 109452445A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a kind of preparation methods of high-purity sea cucumber Gly-His-Lys, the following steps are included: being put into raw material sea cucumber to cleaning basin, addition is equivalent to 5~8 times of sea cucumber volume of pure water, cleaning 1~3 time, carry out cleaning decontamination processing, by the sea cucumber after cleaning decontamination, it is put into disintegrating apparatus and is smashed for the first time, and 2~5 times of pure water is added in the slurry after smashing for the first time, it is put into filter device, once filtered, remove bulky grain insoluble impurities, after the completion of primary filtering, it is placed again into disintegrating apparatus and carries out secondary smash, it is secondary smash after filtering in the filter device that places into, remove the insoluble impurities of smaller particle, and it will finally crush slurry, and slurry is subjected to defibrination process.The preparation method of the high-purity sea cucumber Gly-His-Lys effectively raises the purity of preparation sea cucumber Gly-His-Lys, reduces the influence of impurity in preparation process, ensure that the quality of sea cucumber Gly-His-Lys, meet the needs of production.
Description
Technical field
The invention belongs to food processing technology fields, and in particular to a kind of preparation method of high-purity sea cucumber Gly-His-Lys.
Background technique
Sea cucumber is a kind of important oceanic invertebrate, and the produced sea cucumber in China is with stichopus japonicus, Sea Cucumber, plum blossom ginseng etc.
Main, these ginseng class economic values are very high.Meat quality of sea cucumber is delicate, full of nutrition, is typical high protein, low fat food, has
High nutritive value and medical value is diet good food for the elderly and hypertension, coronary heart disease, hepatitis, sea
The immunity and hematopoiesis function of body had not only can be enhanced in ginseng, but also can inhibit the growth and transfer of human cancer cell, and sea cucumber peptide refers to
The small-molecular peptides obtained after protease hydrolytic and separating-purifying, and after human body beche-de-mer, macromolecular substances are digested and are decomposed
2-3h is needed at small-molecule substance, absorptivity is 60% or so；And sea cucumber Gly-His-Lys, which are entered human body only, needs a few minutes, absorptivity is
100%.
Therefore sea cucumber peptide has higher biological value than other common cucumber products, also increasingly meets present people's
Demand, but the method for existing preparation sea cucumber Gly-His-Lys, the purity of sea cucumber Gly-His-Lys is handed over low after the completion of preparation, inside contain
Other impurities are more, affect the use of sea cucumber Gly-His-Lys.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation methods of high-purity sea cucumber Gly-His-Lys, to solve in above-mentioned background technique
The problem of proposition.
To achieve the above object, the invention provides the following technical scheme:
A kind of preparation method of high-purity sea cucumber Gly-His-Lys, comprising the following steps:
S1, cleaning decontamination: raw material sea cucumber is put into cleaning basin, is added and is equivalent to 5~8 times of sea cucumber volume pure
Water cleans 1~3 time, carries out cleaning decontamination processing；
S2, crushing, defibrination: it by the sea cucumber after cleaning decontamination, is put into disintegrating apparatus and is smashed for the first time, and will beat for the first time
2~5 times of pure water is added in slurry after broken, is put into filter device, is once filtered, and it is insoluble miscellaneous to remove bulky grain
Matter, after the completion of primary filtering, be placed again into disintegrating apparatus carry out it is secondary smash, it is secondary smash after in the filter device that places into
Filtering, removes the insoluble impurities of smaller particle, and will finally crush slurry, and slurry is carried out defibrination process, later will mill
Slurry be heated to 100~110 DEG C, 30~40min of heating time, to defibrination be cooled to 40~50 degrees Celsius it is spare；
S3, enzymatic hydrolysis: defibrination obtained in step S2 is put into enzymatic vessel, and be added with defibrination weight ratio be 0.1~
5% organized enzyme, and blender is adjusted under the revolving speed of 40r/min after mixing evenly, 3~5h is digested, directly enzymatic hydrolysis is completed；
S4, concentrate drying: it is dry after concentrated after hydrolyzate filtering in step S3, obtain powdered high-purity sea
Join Gly-His-Lys.
Preferably, in step S1, raw material sea cucumber is pure wild sea cucumber, and the soaking time before cleaning in pure water is 4
~6 hours, and make the sea cucumber epidermis after cleaning without dirt.
Preferably, in step S2, filter device is filter screen, and the filter mesh of first filter device is greater than secondary mistake
The filter mesh of device is filtered, disintegrating apparatus is meat grinder, and rubbing degree is ground to be advisable by Φ 8mm Minced Steak plate using bone mud
Superfine defibrination is carried out, defibrination fineness is controlled at 5 μm or less.
Preferably, in step S3, organized enzyme uses pepsin, alkali protease, neutral proteinase, trypsase, wood
One of melon protease, hydrolysising protease and compound protease or multiple combinations.
Preferably, it in step S3, after the completion of enzymatic hydrolysis, also needs to be brought rapidly up to 80~90 DEG C, keeps 20~30min, complete
Sterilising and enzyme inactivating processing to hydrolyzate.
Preferably, in step S4, using filtering twice, the mesh number for filtering mesh screen for the first time is 60~80 for the filtering of hydrolyzate
Mesh, second of mesh number for filtering mesh screen is 100~120 mesh.
Preferably, in step S4, concentration process is using vacuum concentration, and being concentrated into solid content is 25%~35%, concentration
Keep hydrolysis temperature at 50~60 DEG C in the process, drying after concentration is dried it using centrifugal spray dryer,
The inlet temperature of spray drying agent is controlled at 200~220 DEG C, outlet temperature is at 90~96 DEG C.
Technical effect and advantage of the invention: the preparation method of the high-purity sea cucumber Gly-His-Lys, is finger protein according to sea cucumber peptide
The small-molecular peptides obtained after enzyme hydrolysis and separating-purifying, and sea cucumber peptide has multiple efficacies, such as lowering blood pressure and blood fat, anti-artery
Atherosis, antifatigue, antibacterial improve immunity, anti-aging and many physiological functions such as anti-oxidant, are being made by sea cucumber peptide
When standby, sea cucumber eliminates sea cucumber when crushing, brought impurity, to facilitate subsequent also sea by repeatedly smashing and filtering
Join the preparation of peptide, and carry out purification processes for sea cucumber peptide, when in addition being filtered after enzymatic hydrolysis, through different degrees of filtering, again
Sea cucumber peptide is purified, other impurities contained when concentration are reduced, be effectively guaranteed sea cucumber peptide dried bean noodles it is dry after the completion of
Purity, and using pure natural pollution-free wild sea cucumber be used as raw material, prepare sea cucumber Gly-His-Lys nutritive value it is higher.
Specific embodiment
Below in conjunction with the contents of the present invention, the technical solution in the present invention is clearly and completely described, it is clear that
Described content is only a part of content of the present invention, rather than whole contents.Based on the content in the present invention, this field
Those of ordinary skill's every other content obtained without making creative work belongs to what the present invention protected
Range.
Embodiment 1
The present invention provides a kind of preparation methods of high-purity sea cucumber Gly-His-Lys, comprising the following steps:
S1, cleaning decontamination: raw material sea cucumber is put into cleaning basin, and addition is equivalent to 5 times of sea cucumber volume of pure water,
Cleaning 1 time carries out cleaning decontamination processing；
S2, crushing, defibrination: it by the sea cucumber after cleaning decontamination, is put into disintegrating apparatus and is smashed for the first time, and will beat for the first time
2 times of pure water is added in slurry after broken, is put into filter device, is once filtered, removing bulky grain insoluble impurities, and one
After the completion of secondary filtering, be placed again into disintegrating apparatus carry out it is secondary smash, it is secondary smash after filtering in the filter device that places into,
The insoluble impurities of smaller particle is removed, and will finally crush slurry, and slurry is subjected to defibrination process, later heats defibrination
To 100 DEG C, heating time 30min, to defibrination be cooled to 40 degrees Celsius it is spare；
S3, enzymatic hydrolysis: defibrination obtained in step S2 is put into enzymatic vessel, and being added with defibrination weight ratio is 0.1
Organized enzyme, and blender is adjusted under the revolving speed of 40r/min after mixing evenly, 3h is digested, directly enzymatic hydrolysis is completed；
S4, concentrate drying: it is dry after concentrated after hydrolyzate filtering in step S3, obtain powdered high-purity sea
Join Gly-His-Lys.
Specifically, raw material sea cucumber is pure wild sea cucumber in step S1, the soaking time before cleaning in pure water is 4
Hour, and make the sea cucumber epidermis after cleaning without dirt.
Specifically, filter device is filter screen in step S2, and the filter mesh of first filter device is greater than secondary mistake
The filter mesh of device is filtered, disintegrating apparatus is meat grinder, and rubbing degree is ground to be advisable by Φ 8mm Minced Steak plate using bone mud
Superfine defibrination is carried out, defibrination fineness is controlled at 5 μm or less.
Specifically, organized enzyme uses pepsin, alkali protease, neutral proteinase, trypsase, wood in step S3
One of melon protease, hydrolysising protease and compound protease or multiple combinations.
Specifically, after the completion of enzymatic hydrolysis, also needing to be brought rapidly up to 80 DEG C in step S3,20min is kept, is completed to hydrolyzate
Sterilising and enzyme inactivating processing.
Specifically, using filtering twice, the mesh number for filtering mesh screen for the first time is 60~80 for the filtering of hydrolyzate in step S4
Mesh, second of mesh number for filtering mesh screen is 100~120 mesh.
Specifically, concentration process is using vacuum concentration, and being concentrated into solid content is 25%, in concentration process in step S4
Keep hydrolysis temperature at 50 DEG C, drying after concentration is dried it using centrifugal spray dryer, controls spraying dry
The inlet temperature of drying prescription is at 200 DEG C, and outlet temperature is at 90 DEG C.
Embodiment 2
A kind of preparation method of high-purity sea cucumber Gly-His-Lys, comprising the following steps:
S1, cleaning decontamination: raw material sea cucumber is put into cleaning basin, and addition is equivalent to 6 times of sea cucumber volume of pure water,
Cleaning 2 times carries out cleaning decontamination processing；
S2, crushing, defibrination: it by the sea cucumber after cleaning decontamination, is put into disintegrating apparatus and is smashed for the first time, and will beat for the first time
4 times of pure water is added in slurry after broken, is put into filter device, is once filtered, removing bulky grain insoluble impurities, and one
After the completion of secondary filtering, be placed again into disintegrating apparatus carry out it is secondary smash, it is secondary smash after filtering in the filter device that places into,
The insoluble impurities of smaller particle is removed, and will finally crush slurry, and slurry is subjected to defibrination process, later heats defibrination
To 105 DEG C, heating time 35min, to defibrination be cooled to 45 degrees Celsius it is spare；
S3, enzymatic hydrolysis: defibrination obtained in step S2 is put into enzymatic vessel, and being added with defibrination weight ratio is 2.5%
Organized enzyme, and blender is adjusted under the revolving speed of 40r/min after mixing evenly, digests 4h, directly enzymatic hydrolysis completion；
S4, concentrate drying: it is dry after concentrated after hydrolyzate filtering in step S3, obtain powdered high-purity sea
Join Gly-His-Lys.
Specifically, raw material sea cucumber is pure wild sea cucumber in step S1, the soaking time before cleaning in pure water is 5
Hour, and make the sea cucumber epidermis after cleaning without dirt.
Specifically, filter device is filter screen in step S2, and the filter mesh of first filter device is greater than secondary mistake
The filter mesh of device is filtered, disintegrating apparatus is meat grinder, and rubbing degree is ground to be advisable by Φ 8mm Minced Steak plate using bone mud
Superfine defibrination is carried out, defibrination fineness is controlled at 5 μm or less.
Specifically, organized enzyme uses pepsin, alkali protease, neutral proteinase, trypsase, wood in step S3
One of melon protease, hydrolysising protease and compound protease or multiple combinations.
Specifically, after the completion of enzymatic hydrolysis, also needing to be brought rapidly up to 85 DEG C in step S3,25min is kept, is completed to hydrolyzate
Sterilising and enzyme inactivating processing.
Specifically, using filtering twice, the mesh number for filtering mesh screen for the first time is 60~80 for the filtering of hydrolyzate in step S4
Mesh, second of mesh number for filtering mesh screen is 100~120 mesh.
Specifically, concentration process is using vacuum concentration, and being concentrated into solid content is 30%, in concentration process in step S4
Keep hydrolysis temperature at 55 DEG C, drying after concentration is dried it using centrifugal spray dryer, controls spraying dry
The inlet temperature of drying prescription is at 210 DEG C, and outlet temperature is at 93 DEG C.
Embodiment 3
A kind of preparation method of high-purity sea cucumber Gly-His-Lys, comprising the following steps:
S1, cleaning decontamination: raw material sea cucumber is put into cleaning basin, and addition is equivalent to 8 times of sea cucumber volume of pure water,
Cleaning 3 times carries out cleaning decontamination processing；
S2, crushing, defibrination: it by the sea cucumber after cleaning decontamination, is put into disintegrating apparatus and is smashed for the first time, and will beat for the first time
5 times of pure water is added in slurry after broken, is put into filter device, is once filtered, removing bulky grain insoluble impurities, and one
After the completion of secondary filtering, be placed again into disintegrating apparatus carry out it is secondary smash, it is secondary smash after filtering in the filter device that places into,
The insoluble impurities of smaller particle is removed, and will finally crush slurry, and slurry is subjected to defibrination process, later heats defibrination
To 110 DEG C, heating time 40min, to defibrination be cooled to 50 degrees Celsius it is spare；
S3, enzymatic hydrolysis: defibrination obtained in step S2 is put into enzymatic vessel, and being added with defibrination weight ratio is 5%
Organized enzyme, and blender is adjusted under the revolving speed of 40r/min after mixing evenly, 5h is digested, directly enzymatic hydrolysis is completed；
S4, concentrate drying: it is dry after concentrated after hydrolyzate filtering in step S3, obtain powdered high-purity sea
Join Gly-His-Lys.
Specifically, raw material sea cucumber is pure wild sea cucumber in step S1, the soaking time before cleaning in pure water is 6
Hour, and make the sea cucumber epidermis after cleaning without dirt.
Specifically, filter device is filter screen in step S2, and the filter mesh of first filter device is greater than secondary mistake
The filter mesh of device is filtered, disintegrating apparatus is meat grinder, and rubbing degree is ground to be advisable by Φ 8mm Minced Steak plate using bone mud
Superfine defibrination is carried out, defibrination fineness is controlled at 5 μm or less.
Specifically, organized enzyme uses pepsin, alkali protease, neutral proteinase, trypsase, wood in step S3
One of melon protease, hydrolysising protease and compound protease or multiple combinations.
Specifically, after the completion of enzymatic hydrolysis, also needing to be brought rapidly up to 90 DEG C in step S3,30min is kept, is completed to hydrolyzate
Sterilising and enzyme inactivating processing.
Specifically, using filtering twice, the mesh number for filtering mesh screen for the first time is 60~80 for the filtering of hydrolyzate in step S4
Mesh, second of mesh number for filtering mesh screen is 100~120 mesh.
Specifically, concentration process is using vacuum concentration, and being concentrated into solid content is 35%, in concentration process in step S4
Keep hydrolysis temperature at 60 DEG C, drying after concentration is dried it using centrifugal spray dryer, controls spraying dry
The inlet temperature of drying prescription is at 220 DEG C, and outlet temperature is at 96 DEG C.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features,
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (7)
1. a kind of preparation method of high-purity sea cucumber Gly-His-Lys, it is characterised in that: the following steps are included:
S1, cleaning decontamination: raw material sea cucumber is put into cleaning basin, and addition is equivalent to 5~8 times of sea cucumber volume of pure water,
Cleaning 1~3 time carries out cleaning decontamination processing；
S2, crushing, defibrination: will clean the sea cucumber after decontamination, be put into disintegrating apparatus and smashed for the first time, and after first smashing
Slurry 2~5 times of pure water is added, be put into filter device, once filtered, removing bulky grain insoluble impurities, one
After the completion of secondary filtering, be placed again into disintegrating apparatus carry out it is secondary smash, it is secondary smash after filtering in the filter device that places into,
The insoluble impurities of smaller particle is removed, and will finally crush slurry, and slurry is subjected to defibrination process, later heats defibrination
To 100~110 DEG C, 30~40min of heating time, to defibrination be cooled to 40~50 degrees Celsius it is spare；
S3, enzymatic hydrolysis: defibrination obtained in step S2 is put into enzymatic vessel, and being added with defibrination weight ratio is 0.1~5%
Organized enzyme, and blender is adjusted under the revolving speed of 40r/min after mixing evenly, 3~5h is digested, directly enzymatic hydrolysis is completed；
S4, concentrate drying: it is dry after concentrated after hydrolyzate filtering in step S3, obtain powdered high-purity sea cucumber peptide
Powder.
2. a kind of preparation method of high-purity sea cucumber Gly-His-Lys according to claim 1, it is characterised in that: former in step S1
Material sea cucumber is pure wild sea cucumber, and the soaking time before cleaning in pure water is 4~6 hours, and makes the sea cucumber table after cleaning
Skin is without dirt.
3. a kind of preparation method of high-purity sea cucumber Gly-His-Lys according to claim 1, it is characterised in that: in step S2, mistake
Filter device is filter screen, and the filter mesh of first filter device is greater than the filter mesh of secondary filter, disintegrating apparatus
For meat grinder, rubbing degree carries out superfine defibrination, defibrination fineness control using bone mud mill to be advisable by Φ 8mm Minced Steak plate
System is at 5 μm or less.
4. a kind of preparation method of high-purity sea cucumber Gly-His-Lys according to claim 1, it is characterised in that: living in step S3
Property enzyme use pepsin, alkali protease, neutral proteinase, trypsase, papain, hydrolysising protease and compound egg
One of white enzyme or multiple combinations.
5. a kind of preparation method of high-purity sea cucumber Gly-His-Lys according to claim 1, it is characterised in that: in step S3, enzyme
It after the completion of solution, also needs to be brought rapidly up to 80~90 DEG C, keeps 20~30min, complete to handle the sterilising and enzyme inactivating of hydrolyzate.
6. a kind of preparation method of high-purity sea cucumber Gly-His-Lys according to claim 1, it is characterised in that: in step S4, water
The filtering of solution liquid uses filters twice, and the mesh number for filtering mesh screen for the first time is 60~80 mesh, and the mesh number of second of filtering mesh screen is
100~120 mesh.
7. a kind of preparation method of high-purity sea cucumber Gly-His-Lys according to claim 1, it is characterised in that: dense in step S4
Compression process is using vacuum concentration, and being concentrated into solid content is 25%~35%, keeps hydrolysis temperature 50~60 in concentration process
DEG C, drying after concentration is dried it using centrifugal spray dryer, and the inlet temperature for controlling spray drying agent exists
200~220 DEG C, outlet temperature is at 90~96 DEG C.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112438356A (en) * | 2019-08-29 | 2021-03-05 | 东北农业大学 | Multi-element compound peptide solid beverage and preparation method thereof |
| CN114794456A (en) * | 2021-01-29 | 2022-07-29 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Instant sea cucumber soup and preparation method thereof |
| CN116606350A (en) * | 2023-07-14 | 2023-08-18 | 山东中科海洋生物工程有限公司 | Sea cucumber peptide and production method and application thereof |
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