CN109452173A - A kind of U.S.'s gold leaf Chinese honey locust breeding method that disease resistance is strong - Google Patents
A kind of U.S.'s gold leaf Chinese honey locust breeding method that disease resistance is strong Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The present invention provides a kind of U.S.'s gold leaf Chinese honey locust breeding method that disease resistance is strong, including chooses explant, Primary culture, differentiation culture, domestication step；The selection explant: the explant of clip is the terminal bud stem section of the vigorous branch of tree crown top peripheral, it is desirable that terminal bud is full, and stem section length is 1~1.8cm；The Primary culture: pretreated explant is put into Primary culture base, Primary culture；The differentiation culture: terminal bud is inoculated on differential medium, be placed in light application time be 11 ± 0.5h/d, intensity of illumination be 3100 ± 200Lx, culture room temperature be 27 ± 0.5 DEG C under conditions of cultivate 28 ± 1 days, obtain a large amount of Multiple Buds.It is strong that the U.S. gold leaf Chinese honey locust that breeding method of the present invention obtains cultivates diseases and insect pests resistance.
Description
The present invention is application number 201710651830.4, and the applying date on 08 02nd, 2017, " a kind of U.S. was golden for denomination of invention
The divisional application of leaf Chinese honey locust tissue culture and rapid propagation method ".
Technical field
The present invention relates to a kind of U.S.'s gold leaf Chinese honey locust mating systems, and in particular to a kind of U.S.'s gold leaf soap that disease resistance is strong
Pod breeding method belongs to breeding plant technical field.
Background technique
U.S. gold leaf Chinese honey locust, broadleaf deciduous arbor, 9~10.5 meters of plant height, growth is very fast, and no scolus, spire is golden yellow,
Gold leaf Chinese honey locust climax leaves chartreuse, until autumn is still chartreuse, branch is unfolded, and plant type is beautiful, shaky.
Property light and slightly it is resistance to it is shady, drought-enduring, cold-resistant, be resistant to -34 spend low temperature.Like warm and moist weather and deep fertile soil
Earth can also be grown in calcium carbonate and slight saline-alkali soil, and adaptability is wider, and NORTH CHINA to south and the west and south can plant
It plants.Deep-rootedness, few pest and disease damage.It is found in production, torticollis is easy in diameter of a cross-section of a tree trunk 1.3 meters above the ground 3cm plant strain growth below.
For northern area due to very cold, plant variety is single, and color leafed plants lack, and arbor type Colored- leaf Plants especially lack
It is weary, gold leaf Chinese honey locust it is leaf it is beautiful, leaf color is golden yellow, its successful popularization, will greatly enrich this area landscape effect.Current
U.S.'s gold leaf Chinese honey locust raising technology is mostly to graft, but due to growing comparatively fast, easily snap off at grafting, resistance is not strong；And tissue culture
The U.S. gold leaf Chinese honey locust of breeding not only overcomes drawbacks described above, but also breeds quickly, and seedling strain is virus-free, and diseases and insect pests resistance is strong.
It now there is no the report of U.S.'s gold leaf Chinese honey locust group culturation rapid propagating technology in the art.
Summary of the invention
In view of the deficiencies of the prior art, numerous the object of the present invention is to provide a kind of U.S.'s gold leaf Chinese honey locust tissue culture and rapid propagation method
It is fast to grow speed, high survival rate, and the U.S.'s gold leaf Chinese honey locust diseases and insect pests resistance cultivated is strong, plant is not susceptible to torticollis.
To solve existing technical problem, the technical solution adopted by the present invention is that:
A kind of U.S.'s gold leaf Chinese honey locust tissue culture and rapid propagation method, including choose explant step；The selection explant: explant choosing
Take the terminal bud stem section of the vigorous branch of tree crown top peripheral, it is desirable that terminal bud is full, and stem section length is 1~1.8cm；With scissors clip
Explant, environment temperature is 22~28 DEG C when clip explant, and humidity is 65~70%.
The method further includes explant sterilisation step；The explant disinfection: (1) explant is placed in triangular flask
It is interior, 75% 1~2min of alcohol disinfecting is added, then with clear water repeated flushing 3 times, rinse well；By explant with 0.1% mercuric chloride
Sterilize 5~10min；Aseptic water washing 8 times.
The method further includes pre-treatment step；The pretreatment: by gibberellin, 6-BA, IBA, potassium dihydrogen phosphate
According to the aqueous solution for being configured to 0.05 mg/L after the ratio mixing of 2:1:2:3, pretreatment fluid is obtained；It then will be outer after disinfection
Implant, which is put into pretreatment fluid, impregnates 8 hours.
The method further includes Primary culture step；The Primary culture:
Control condition of culture are as follows: 28 ± 0.5 DEG C of environment temperature of control, light application time are 5 ± 0.5 h/d, intensity of illumination 2700
~3000 Lx, humidity are 60~65%.
The method further includes breaking up incubation step, the differentiation culture:
The differential medium of use are as follows: 0.05 mg/L+ honey 8g/L+ agar 9 of MS culture medium+NAA 0.15mg/L+IAA
G/L, adjusting pH is 6.5.
The method further includes cultivating tissue-cultured seedling step, the cultivation tissue-cultured seedling:
The Multiple Buds that differentiation culture obtains are inoculated on strengthening seedling and rooting culture medium, the strengthening seedling and rooting culture medium is MS culture
Base+6-BA 0.08mg/L+ NAA 0.12mg/L+pollen pini 0.25mg/L+folic acid 0.1mg/L+9 g/L of agar is adjusted
PH is 6.5.
The cultivation tissue-cultured seedling:
By the culture medium for being inoculated with Multiple Buds be placed in light application time be 10h/d, intensity of illumination 3300Lx, culture room temperature be 28 DEG C
Under conditions of cultivate 25~30 days, obtain tissue-cultured seedling.
The method further includes hardening step, the hardening:
By tissue culture transplantation of seedlings in culture substrate, overlay film, under conditions of intensity of illumination is 3200 Lx, controlled at 28~
30 DEG C, humidity is 65~70%, hardening culture 33~35 days, obtains the good seedling of root system development.
The culture substrate is by vermiculite, perlite, jute, cinnamomi cortex pulveratus, graphene, turf according to 10:7:5:1:4:3's
Mass ratio mixing, sprays into water, and control water content is 45%.
The method further includes domestication step；The domestication:
Controlling ambient humidity is 65~70%, and intensity of illumination is 3100~3300Lx, and light application time is 8~10h/d, air flowing
Amount is 100~120m3/h；Use airconditioning control environment temperature: day temperature is warming up to 28 DEG C from 18 DEG C of speed with 2 DEG C/h,
It being kept for 28 DEG C two hours, then is cooled to 18 DEG C with the speed of 2 DEG C/h, nocturnal temperature is cooled to 12 DEG C with the speed of 1 DEG C/h, then with
The speed of 1 DEG C/h is warming up to 18 DEG C；Continuous domestication 21~25 days.
By adopting the above-described technical solution, having the technical effect that of reaching of the present invention
(1) gold leaf Chinese honey locust tissue culture and rapid propagation method in the U.S.'s of the present invention is used, survival rate of plant improves 15.5%, up to 99.5%；
(2) gold leaf Chinese honey locust tissue culture and rapid propagation method in the U.S.'s of the present invention is used, plant strain growth speed is fast, transplants the plant height of current year
Increase by 98~105cm；
(3) U.S.'s gold leaf Chinese honey locust disease resistance cultivated using gold leaf Chinese honey locust tissue culture and rapid propagation method in the U.S.'s of the present invention is strong, is not easy to send out
Raw Disease, diameter of a cross-section of a tree trunk 1.3 meters above the ground 3cm plant occur without torticollis symptom；The insect pest incidence of longicorn lower than common plant 12.5%；It is resistant to
Subzero 40 DEG C of low temperature.
Specific embodiment
A kind of U.S.'s gold leaf Chinese honey locust tissue culture and rapid propagation method of embodiment 1
1, material prepares
It tests and was carried out in 2015 at Weifang Vocatonal College's tissue culture center, test material is U.S.'s gold leaf Chinese honey locust (Gleditsla
Triacanthos ' Sunburst ').
2, explant is chosen
Vigorous, no disease and pests harm, the excellent U.S.'s gold leaf Chinese honey locust single plant of trait expression are grown in outdoor selection；With scissors clip explant
Body, environment temperature is 25 DEG C when clip explant, humidity 66%；
The explant of clip are as follows: the terminal bud stem section of the vigorous branch of tree crown top peripheral, it is desirable that terminal bud is full, and stem section length is
1.0cm。
3, explant sterilizes
Explant is placed in triangular flask, 75% alcohol disinfecting 1min is added, then with clear water repeated flushing 3 times, rinse well；
In superclean bench, 0.1% mercuric chloride of explant is sterilized into 7min；It is aseptic water washing 8 times, spare.
4, it pre-processes
The water-soluble of 0.05 mg/L is configured to after gibberellin, 6-BA, IBA, potassium dihydrogen phosphate are mixed according to the ratio of 2:1:2:3
Liquid obtains pretreatment fluid；Then the explant after disinfection is put into pretreatment fluid and is impregnated 8 hours.
5, Primary culture
The Primary culture base of use are as follows:
Primary culture base is prepared according to formula as below with distilled water:
NH4NO3 1.650 g/L、KNO3 1.900 g/L、CaCl2·2H2O 440 mg/L、MgSO4·7H2O 370 mg/L、
KH2PO4 700 mg/L、KI 0.83 mg/L、H3BO3 6.2 mg/L、MnSO4·4H2O 22.3 mg/L、ZnSO4·7H2O
8.6 mg/L、Na2MnO4·2H2O 0.25 mg/L、CuSO4·5H2O 0.025 mg/L、CoCl2·6H2O 0.025 mg/L、
FeSO4·7H2O 27.8 mg/L、Na2-EDTA·2H237.3 mg/L of O, inositol 85mg/L, 1.1 mg/L of niacin, hydrochloric acid
0.2 mg/L of pyridoxol, 0.8 mg/L of thiamine hydrochloride, 0.05 mg/L of aspartic acid 1.2mg/L, 6-BA, heteroauxin
0.08 mg/L, 0.85 g/L of chlorogenic acid, 9 g/L of agar.
The prepared whole pH of Primary culture keynote is 6.8；By high pressure steam sterilization, and 25 DEG C are cooled to, it is spare.
Pretreated explant is put into Primary culture base, Primary culture；Control condition of culture are as follows: control environment temperature
28 ± 0.5 DEG C of degree, light application time are 5 h/d, and intensity of illumination is 2700 Lx, humidity 60%.
Incubation time is 20 days, and terminal bud is scaled off from bastem portion, spare.
6, differentiation culture
Terminal bud is inoculated on differential medium, differential medium are as follows: 0.05 mg/ of MS culture medium+NAA 0.15mg/L+IAA
9 g/L of L+ honey 8g/L+ agar, adjusting pH is 6.5；
It is placed in light application time to be 11h/d, intensity of illumination 3100Lx, cultivate under conditions of room temperature is 27 DEG C and cultivate 28 days, obtain
To a large amount of Multiple Buds, i.e. adventitious bud, single Multiple Buds are dispersed out, it is spare.
7, tissue-cultured seedling is cultivated
Multiple Buds are inoculated on strengthening seedling and rooting culture medium, the strengthening seedling and rooting culture medium is MS culture medium+6-BA
0.02 mg/L+ pollen pini 0.25mg/L of 0.08mg/L+ NAA 0.12mg/L+cod-liver oil+folic acid 0.1mg/L+agar 9
G/L, adjusting pH is 6.5；
It is placed in light application time to be 10h/d, intensity of illumination 3300Lx, cultivate under conditions of room temperature is 28 DEG C and cultivate 25 days, obtain
To tissue-cultured seedling.
8, hardening
By tissue culture transplantation of seedlings in culture substrate, overlay film, under conditions of intensity of illumination is 3200 Lx, controlled at 28 DEG C,
Humidity is 65%, hardening culture 33 days, obtains the good seedling of root system development；
The culture substrate by vermiculite, perlite, jute, cinnamomi cortex pulveratus, graphene, turf according to 10:7:5:1:4:3 quality
Than mixing, water is sprayed into, control water content is 45%.
9, it tames
By seedling replanting into greenhouse, when transplanting, the seedling cave of 0.4m is dug, 2g root-inducing powder is sprinkled into seedling cave and soil mixes, is put into children
Seedling, earthing cover seedlings root, pour primary permeable, and control ambient humidity is 65%, intensity of illumination 3300Lx, and light application time is
10h/d, air flow amount 120m3/h；
Use airconditioning control environment temperature: day temperature is warming up to 28 DEG C from 18 DEG C of speed with 2 DEG C/h, keeps 28 DEG C two small
When, then 18 DEG C are cooled to the speed of 2 DEG C/h, nocturnal temperature is cooled to 12 DEG C with the speed of 1 DEG C/h, then with the speed of 1 DEG C/h
It is warming up to 18 DEG C；
Continuous domestication 20~22 days.
Influence experiment of the explant specification that embodiment 2 is chosen to survival rate
Tissue culture propagation is carried out according to the method for embodiment 1, only changes the step the explant specification chosen in 2, i.e. terminal bud stem section is long
Degree carries out embodiment 2~4, is specifically shown in Table 1:
The explant specification that table 1 is chosen
The tissue-cultured seedling of investigation Examples 1 to 4 survives situation；
Investigation result is shown in Table 2；
2 tissue culture shoot survival percent of table
Investigation result shows that the tissue culture shoot survival percent highest of embodiment 3 is preferred embodiment；The explant chosen in step 2
Specification, terminal bud stem section length is preferably 1.8cm.
Influence of the different strengthening seedling and rooting culture mediums of embodiment 5 to tissue-cultured seedling
Carry out tissue culture propagation according to the method for embodiment 1, only change the step the strengthening seedling and rooting culture medium in 7, carry out embodiment 5~
8:
The strengthening seedling and rooting culture medium that embodiment 5 uses are as follows:
MS culture medium+6-BA 0.08mg/L+ NAA 0.12mg/L+0.02 mg/L+ pollen pini 0.25mg/L of cod-liver oil+fine jade
9 g/L of rouge；
The strengthening seedling and rooting culture medium that embodiment 6 uses are as follows:
MS culture medium+6-BA 0.08mg/L+ NAA 0.12mg/L+pollen pini 0.25mg/L+folic acid 0.1mg/L+agar 9
g/L；
The strengthening seedling and rooting culture medium that embodiment 7 uses are as follows:
MS culture medium+6-BA 0.08mg/L+ IBA 0.12mg/L+0.02 mg/L+ pollen pini 0.25mg/L of cod-liver oil+fine jade
9 g/L of rouge；
The strengthening seedling and rooting culture medium that embodiment 8 uses are as follows:
MS culture medium+6-BA 0.08mg/L+ NAA 0.12mg/L+0.02 mg/L of cod-liver oil+folic acid 0.1mg/L+agar
9 g/L；
The tissue-cultured seedling growing state statistical result that embodiment 5~8 is cultivated is shown in Table 3；
3 tissue-cultured seedling growing state of table
As seen from the above table, the tissue-cultured seedling growth of embodiment 6 is obvious very fast, is preferred embodiment, i.e. strengthening seedling and rooting culture medium is preferred
Are as follows: MS culture medium+6-BA 0.08mg/L+ NAA 0.12mg/L+pollen pini 0.25mg/L+folic acid 0.1mg/L+agar 9
g/L。
A kind of U.S.'s gold leaf Chinese honey locust tissue culture and rapid propagation method of embodiment 9
1, material prepares
It tests and was carried out in 2015 at Weifang Vocatonal College's tissue culture center, test material is U.S.'s gold leaf Chinese honey locust (Gleditsla
Triacanthos ' Sunburst ').
2, explant is chosen
Vigorous, no disease and pests harm, the excellent U.S.'s gold leaf Chinese honey locust single plant of trait expression are grown in outdoor selection；With scissors clip explant
Body, environment temperature is 25 DEG C when clip explant, humidity 66%；
The explant of clip are as follows: the terminal bud stem section of the vigorous branch of tree crown top peripheral, it is desirable that terminal bud is full, and stem section length is
1.8cm。
3, explant sterilizes
Explant is placed in triangular flask, 75% alcohol disinfecting 1min is added, then with clear water repeated flushing 3 times, rinse well；
In superclean bench, 0.1% mercuric chloride of explant is sterilized into 7min；It is aseptic water washing 8 times, spare.
4, it pre-processes
The water-soluble of 0.05 mg/L is configured to after gibberellin, 6-BA, IBA, potassium dihydrogen phosphate are mixed according to the ratio of 2:1:2:3
Liquid obtains pretreatment fluid；Then the explant after disinfection is put into pretreatment fluid and is impregnated 8 hours.
Through testing, compared with without pretreated explant, by the group of the pretreated explant culture of the present invention
Training shoot survival percent is obviously higher by 23% or so, and the speed of growth is very fast.
5, Primary culture
Primary culture base is prepared according to formula as below with distilled water:
NH4NO3 1.650 g/L、KNO3 1.900 g/L、CaCl2·2H2O 440 mg/L、MgSO4·7H2O 370 mg/L、
KH2PO4 700 mg/L、KI 0.83 mg/L、H3BO3 6.2 mg/L、MnSO4·4H2O 22.3 mg/L、ZnSO4·7H2O
8.6 mg/L、Na2MnO4·2H2O 0.25 mg/L、CuSO4·5H2O 0.025 mg/L、CoCl2·6H2O 0.025 mg/L、
FeSO4·7H2O 27.8 mg/L、Na2-EDTA·2H237.3 mg/L of O, inositol 85mg/L, 1.1 mg/L of niacin, hydrochloric acid
0.2 mg/L of pyridoxol, 0.8 mg/L of thiamine hydrochloride, 0.05 mg/L of aspartic acid 1.2mg/L, 6-BA, heteroauxin
0.08 mg/L, 0.85 g/L of chlorogenic acid, 9 g/L of agar.
The prepared whole pH of Primary culture keynote is 6.8；By high pressure steam sterilization, and 25 DEG C are cooled to, it is spare.
Pretreated explant is put into Primary culture base, Primary culture；Control condition of culture are as follows: control environment temperature
28 ± 0.5 DEG C of degree, light application time are 5 h/d, and intensity of illumination is 2700 Lx, humidity 60%.
Incubation time is 20 days, and terminal bud is scaled off from bastem portion, spare.
6, differentiation culture
Terminal bud is inoculated on differential medium, differential medium are as follows: 0.05 mg/ of MS culture medium+NAA 0.15mg/L+IAA
9 g/L of L+ honey 8g/L+ agar, adjusting pH is 6.5；
It is placed in light application time to be 11h/d, intensity of illumination 3100Lx, cultivate under conditions of room temperature is 27 DEG C and cultivate 28 days, obtain
To a large amount of Multiple Buds, i.e. adventitious bud, single Multiple Buds are dispersed out, it is spare.
7, tissue-cultured seedling is cultivated
Multiple Buds are inoculated on strengthening seedling and rooting culture medium, the strengthening seedling and rooting culture medium is MS culture medium+6-BA
0.08mg/L+ NAA 0.12mg/L+pollen pini 0.25mg/L+folic acid 0.1mg/L+9 g/L of agar, adjusting pH is 6.5；
It is placed in light application time to be 10h/d, intensity of illumination 3300Lx, cultivate under conditions of room temperature is 28 DEG C and cultivate 25 days, obtain
To tissue-cultured seedling.
8, hardening
By tissue culture transplantation of seedlings in culture substrate, overlay film, under conditions of intensity of illumination is 3200 Lx, controlled at 28 DEG C,
Humidity is 65%, hardening culture 33 days, obtains the good seedling of root system development；
The culture substrate by vermiculite, perlite, jute, cinnamomi cortex pulveratus, graphene, turf according to 10:7:5:1:4:3 quality
Than mixing, water is sprayed into, control water content is 45%.
9, it tames
By seedling replanting into greenhouse, when transplanting, the seedling cave of 0.4m is dug, 2g root-inducing powder is sprinkled into seedling cave and soil mixes, is put into children
Seedling, earthing cover seedlings root, pour primary permeable, and control ambient humidity is 65%, intensity of illumination 3300Lx, and light application time is
10h/d, air flow amount 120m3/h；
Use airconditioning control environment temperature: day temperature is warming up to 28 DEG C from 18 DEG C of speed with 2 DEG C/h, keeps 28 DEG C two small
When, then 18 DEG C are cooled to the speed of 2 DEG C/h, nocturnal temperature is cooled to 12 DEG C with the speed of 1 DEG C/h, then with the speed of 1 DEG C/h
It is warming up to 18 DEG C；
Continuous domestication 21 days.
Through testing, the tissue culture method of the present embodiment is compared to conventional engrafting method: survival rate of plant raising 15.5%, reachable
99.5%；Plant strain growth speed is fast, and the plant height for transplanting current year increases 98-105cm；
Through survey: U.S.'s gold leaf Chinese honey locust diseases and insect pests resistance that the present embodiment is cultivated is strong, is not susceptible to Disease, chest
Diameter 3cm plant occurs without torticollis symptom；The insect pest incidence of longicorn lower than common plant 12.5%；It is resistant to -40 DEG C of low temperature.Unless
Specified otherwise and unit commonly used in the art, ratio of the present invention are mass ratio, the percentage, are quality percentage
Than.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (7)
1. a kind of U.S.'s gold leaf Chinese honey locust breeding method that disease resistance is strong, it is characterised in that: including choosing explant, starting training
It supports, differentiation culture, domestication step；
The selection explant: the explant of clip is the terminal bud stem section of the vigorous branch of tree crown top peripheral, it is desirable that terminal bud
Full, stem section length is 1~1.8cm；
The Primary culture: pretreated explant is put into Primary culture base, Primary culture；
The differentiation culture: terminal bud is inoculated on differential medium, and being placed in light application time is 11 ± 0.5h/d, intensity of illumination
It is cultivated 28 ± 1 days under conditions of being 27 ± 0.5 DEG C for 3100 ± 200Lx, culture room temperature, obtains a large amount of Multiple Buds.
2. a kind of strong U.S.'s gold leaf Chinese honey locust breeding method of disease resistance according to claim 1, it is characterised in that: described
Selection explant: when clip explant environment temperature be 22~28 DEG C, humidity be 65~70%.
3. a kind of strong U.S.'s gold leaf Chinese honey locust breeding method of disease resistance according to claim 1, it is characterised in that: described
Primary culture base include following components: NH4NO3 1.650 g/L、KNO3 1.900 g/L、CaCl2·2H2O 440 mg/L、
MgSO4·7H2O 370 mg/L、KH2PO4 700 mg/L、KI 0.83 mg/L、H3BO3 6.2 mg/L、MnSO4·4H2O
22.3 mg/L、ZnSO4·7H2O 8.6 mg/L、Na2MnO4·2H2O 0.25 mg/L、CuSO4·5H2O 0.025 mg/L、
CoCl2·6H2O 0.025 mg/L、FeSO4·7H2O 27.8 mg/L、Na2-EDTA·2H237.3 mg/L of O, inositol
85mg/L, 1.1 mg/L of niacin, 0.2 mg/L of puridoxine hydrochloride, 0.8 mg/L of thiamine hydrochloride, aspartic acid 1.2mg/L,
0.05 mg/L of 6-BA, 0.08 mg/L of heteroauxin, 0.85 g/L of chlorogenic acid, 9 g/L of agar.
4. a kind of strong U.S.'s gold leaf Chinese honey locust breeding method of disease resistance according to claim 1, it is characterised in that: described
Primary culture: Primary culture base pH be 6.8.
5. a kind of strong U.S.'s gold leaf Chinese honey locust breeding method of disease resistance according to claim 1, it is characterised in that: described
Primary culture: control 28 ± 0.5 DEG C of environment temperature, light application time be 5 ± 0.5 h/d, intensity of illumination be 2700~3000
Lx, humidity are 60~65%.
6. a kind of strong U.S.'s gold leaf Chinese honey locust breeding method of disease resistance according to claim 1, it is characterised in that: described
Primary culture: incubation time be 20 days.
7. a kind of strong U.S.'s gold leaf Chinese honey locust breeding method of disease resistance according to claim 1, it is characterised in that: described
Differential medium are as follows: 0.05 mg/L+ honey 8g/L+ agar of MS culture medium+NAA 0.15mg/L+IAA 9 g/L, pH are
6.5。
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| CN201811556235.3A CN109452173A (en) | 2017-08-02 | 2017-08-02 | A kind of U.S.'s gold leaf Chinese honey locust breeding method that disease resistance is strong |
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| CN201811556235.3A Pending CN109452173A (en) | 2017-08-02 | 2017-08-02 | A kind of U.S.'s gold leaf Chinese honey locust breeding method that disease resistance is strong |
| CN201811557434.6A Pending CN109566413A (en) | 2017-08-02 | 2017-08-02 | A kind of breeding method of low temperature resistant U.S.'s gold leaf Chinese honey locust |
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| CN201811556236.8A Pending CN109430057A (en) | 2017-08-02 | 2017-08-02 | A kind of U.S.'s gold leaf Chinese honey locust tissue culture and rapid propagation method of high survival rate |
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| CN112400696B (en) * | 2020-12-28 | 2022-04-08 | 内蒙古蒙草生态环境（集团）股份有限公司 | Tissue culture method of evergreen common selfheal fruit-spike bamboo |
| CN112400695B (en) * | 2020-12-28 | 2022-04-08 | 内蒙古蒙草生态环境（集团）股份有限公司 | Culture medium for culturing evergreen common summer pink |
| CN115413577B (en) * | 2022-08-31 | 2023-01-24 | 河北农业大学 | Tissue culture and rapid propagation method for gleditschia horrida |
| CN116458427B (en) * | 2023-04-14 | 2024-01-26 | 广西壮族自治区农业科学院 | Method for producing root-knot potatoes in asparagus bottle |
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| CN105766655B (en) * | 2016-04-28 | 2018-07-10 | 湖南师范大学 | The method for building up of villus Chinese honey locust Tissue Culture Regeneration System |
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