A kind of spot-eliminating beauty treatment soap and preparation method thereof
Technical field
The present invention relates to filed of daily-use chemical industry more particularly to a kind of spot-eliminating beauty treatment composition and its preparation method and application.
Background technique
Color spot is the increase due to dermal melanin and the common face of one kind that is formed is brown or melanin pigmentation, damages
The skin disease of capacitive is mainly in cheek and forehead position, aggravates after solarization, be more common in women.Currently, the flower of method five of nti-freckle
Eight, such as freckle-removing mask, laser nti-freckle, freckle-removing cream be all kinds of.Among these, beauty soap is also because easy to use, at it
While basic face cleaning decontamination, it is used for a long time, good effect can be also played to nti-freckle, pursued by people.
However traditional beauty soap, facial soap, nti-freckle soap etc. are often by animal or vegetable fat and excessive alkali
It is prepared, in order to reach certain spot-eliminating beauty treatment and better washing effect, the change of part is also had in some soap bodies
Component additive agent is learned, although having stronger decontamination, ability of deoiling, be easy to cause skin dry and astringent, tight, over time can
Certain injury is caused to skin, and if things go on like this, after skin absorbs, skin damage, cell viability is be easy to cause to reduce,
Phenomena such as colour of skin is obscure, the cosmetic results such as nti-freckle played are extremely limited.
Therefore, recurrence is natural, mildly also becomes current people using organic component, performance tells spot-eliminating beauty treatment soap
It asks.
Summary of the invention
Above-mentioned to solve the problems, such as, the present invention provides a kind of spot-eliminating beauty treatment soap and preparation method thereof.It is prepared in soap base
In the process, suitable potassium hydroxide is added, and Radix Salviae Miltiorrhizae is added to and prepares soap base mixed liquor of the invention in soap base, contains in Radix Salviae Miltiorrhizae
There is ginsenoside, can be compounded with soap base, washing effect can not only be improved, cleaning is not tight, and to excitation skin cell vitality
Advantageously, while having the function of improving cellular immunity.
In addition, being also added with the concentrated liquid of tradition Chinese medicine in spot-eliminating beauty treatment soap of the invention, contain Chinese medicine material in the concentrated liquid of tradition Chinese medicine
Including rhizome of chuanxiong, Radix Angelicae Sinensis, turmeric and gingko.Rhizoma Chuanxiong is warm-natured, is recorded according to " Japan hanako materia medica ", controls a wind cutting, all gas, all labor
Damage all blood, mends the exhaustion or lesion of the five internal organs, strengthens the bone, adjust many arteries and veins, break crux place blood, support new blood, put on flesh, profuse nasal bleeding, haematemesis and excessive blood, hemorrhoid complicated by anal fistula, brain
Carbuncle carbuncle on the back, scrofula goitre be superfluous, sore scabies and apocenosis dispersing blood stasis blood;Radix Angelicae Sinensis is sweet, pungent, warm, returns liver, the heart, the spleen channel, and activating microcirculation and removing stasis medicinal, scattered silt are stopped
Bitterly, it can be used for ulcer sores, have effects that apocenosis myogenic;There is antibacterial, oxidation resistant effect containing curcumin in turmeric;It is white
Fruit, it is sweet taste, cold in nature, it is recorded according to " book on Chinese herbal medicine is new again ", enters the heart, lung, kidney three and pass through, there is tonifying Qi nourishing heart, kidney-nourishing enriching yin, cough-relieving removes
Tired, myogenic is put on flesh, apocenosis detoxification, the effect of the sore scabies that disappear subcutaneous ulcer tumor, in addition, verifying through the modern times, dimension life also rich in gingko
Plain C, riboflavin, carrotene etc., the integrality with anti-aging, protection cell, have both the effect of antiallergy, anti-inflammation and sterilization.
These traditional Chinese medicine ingredients act on fleshy exterior, and transport through cuticula transhipment and epidermis deep layer and absorb or stimulate fleshy exterior
Or by aquation, circulation channels and collaterals blood vessels are interior to reach internal organs, from outward appearance to inner essence, thus generate effect.Dredging the channel, work can be played
Bloodization silt, adjustment yin-yang, coordinates internal organs, current qi and blood, supports the health maintenance effects such as whole body clearing heat and detoxicating, swelling and pain relieving.Pass through medicine
Reason experiment is also it can be confirmed that the elasticity of skin, vigor, whitening degree are all promoted.
But above-mentioned various traditional Chinese medicine ingredients are when being used alone, the washing face cleaning that plays, freckle effect are limited, do not meet
At this stage to the demand of nti-freckle facial soap, and work as it and mix use, using the synergistic effect of various composition, not only improves
Various substances itself are active, and respective dispersion performance is also more good, and face cleaning, the ability of lightening the stain significantly increase, compound
Soap body performance it is mild and promote the effect of the metabolism of Skin Cell, activated cell vigor so that skin exquisiteness is glossy.
There are a small amount of greases for skin, keep skin oil-water balance, do not dry.
Specifically, the present invention is achieved through the following technical solutions:
In the first aspect of the present invention, the present invention provides a kind of spot-eliminating beauty treatment soap, raw material includes or by 75-90 weight
Part soap base mixture, 1-20 parts by weight the concentrated liquid of tradition Chinese medicine composition, wherein soap base mixture is the mixture of soap base and Radix Salviae Miltiorrhizae.
Preferably, the composition includes or by 77-88 parts by weight soap base mixture, 7-20 parts by weight the concentrated liquid of tradition Chinese medicine group
At.
Preferably, in the soap base mixture, mass ratio 1:99~99:1 of soap base and Radix Salviae Miltiorrhizae, preferably 1-99:1, more
Preferably 5-20:1, most preferably 10:1.
Preferably, the partial size of the Radix Salviae Miltiorrhizae is that can cross 200 meshes.
Preferably, the soap base is selected from the compound soap of animal soap base, plant soap base or animal soap base and plant soap base composition
Base.
Preferably, the mass ratio of grease and potassium hydroxide is 10:1-1.5, preferably 10:1-1.2 in the soap base;
Preferably, the animal soap base is soap base made from animal fat, is selected from butter soap base, lard soap base, sheep oil soap
One of base is a variety of;Soap base made from the plant soap base vegetable fat is selected from coconut oil soap base and/or palm oil soap
Base;
Preferably, in the animal soap base, the mass ratio of animal fat and potassium hydroxide is 10:1;
Preferably, in the vegetable fat, the mass ratio of vegetable fat and potassium hydroxide is 10:1.2;
Preferably, in compound soap base, the mass ratio of grease and potassium hydroxide is 10:1.1;
Preferably, when the soap base is the compound soap base that animal soap base and plant soap base form, animal soap base and plant soap
The mass ratio of base is 1:1-5, preferably 1:1-2.
Preferably, the concentrated liquid of tradition Chinese medicine includes or is made of the Chinese medicine material of following parts by weight: 5-20 parts of rhizome of chuanxiongs, 5-
20 parts of Radix Angelicae Sinensis, 5-20 portions of turmerics, 5-20 portions of gingkoes.
Preferably, the concentrated liquid of tradition Chinese medicine includes or is made of the Chinese medicine material of following parts by weight: 8-15 parts of rhizome of chuanxiongs, 8-
15 parts of Radix Angelicae Sinensis, 8-15 portions of turmerics, 8-15 portions of gingkoes.
Preferably, the concentrated liquid of tradition Chinese medicine includes or is made of the Chinese medicine material of following parts by weight: 10 parts of rhizome of chuanxiongs, 10 parts work as
Return, 10 portions of turmerics, 10 portions of gingkoes.
In the second aspect of the present invention, the present invention also provides a kind of method for preparing above-mentioned beauty soap, the method packets
It includes: after preparing soap base mixture and the concentrated liquid of tradition Chinese medicine respectively, traditional Chinese drug inspissation filtrate being stirred and is added in soap base mixture, continues to stir
Mix, maintain the temperature at 60-70 DEG C, reduced vacuum is evacuated to mixture in paste, pour into later it is cooling in template, soap blank.
Preferably, the soap base mixture is made by the steps: in proportion mixing soap base and Radix Salviae Miltiorrhizae, is heated to
It 55-65 DEG C, after soap base melts completely, stirs and maintains temperature 1 hour, uniform soap base mixture is made.
Preferably, mass ratio 1:99~99:1 of soap base and Radix Salviae Miltiorrhizae, preferably 1-99:1, more preferably 5-20:1 are optimal
It is selected as 10:1.
Preferably, the Radix Salviae Miltiorrhizae is the DANSHEN KELI that partial size can cross 200 meshes.
Preferably, the soap base is selected from the compound soap of animal soap base, plant soap base or animal soap base and plant soap base composition
Base.
Preferably, the mass ratio of grease and potassium hydroxide is 10:1-1.5, preferably 10:1-1.2 in the soap base;
Preferably, the animal soap base is soap base made from animal fat, is selected from butter soap base, lard soap base, sheep oil soap
One of base is a variety of;Soap base made from the plant soap base vegetable fat is selected from coconut oil soap base and/or palm oil soap
Base;
Preferably, in the animal soap base, the mass ratio of animal fat and potassium hydroxide is 10:1;
Preferably, in the vegetable fat, the mass ratio of vegetable fat and potassium hydroxide is 10:1.2;
Preferably, in compound soap base, the mass ratio of grease and potassium hydroxide is 10:1.1;
Preferably, when the soap base is the compound soap base that animal soap base and plant soap base form, animal soap base and plant soap
The mass ratio of base is 1:1-5, preferably 1:1-2.
Preferably, the preparation method of the plant soap base or animal soap base include: weigh vegetable fat or animal fat into
Row heating, is heated to 55-65 DEG C, after vegetable fat or animal fat melt, potassium hydroxide aqueous solution is added dropwise, stirring is simultaneously
Holding temperature 1 hour, for use.
Preferably, the concentrated liquid of tradition Chinese medicine is made by the steps:
Chinese medicine material plus clear water to be impregnated, decocted 2 times, filter residue is filtered out, concentration filtrate to volumetric concentration is 30-60%,
Concentrate is concentrated into the 30-60% of original volume.
Preferably, the concentrated liquid of tradition Chinese medicine is made by the steps:
Chinese medicine material is put into the casserole of crystallization, distilled water is added, cold water soak 30-60 minutes, is impregnated with medicinal material
For principle, amount of water is 5-8 times of medicinal material amount when first pass decocts, heating stirring, since be boiled by fire be changed to after small fire count
Decocting time is calculated, decocts 20-30 minutes, filters out while hot;Second time decoction is added cold water to being higher than about 1-2 centimetres of medicine plane, adds
Thermal agitation, since be boiled by fire be changed to after small fire calculate decocting time, decoct 10-20 minutes, filter out while hot;Merging filtrate,
It is 30-60% that filtrate to concentration, which is concentrated,.
The present invention is pure Chinese medicinal preparation, Nantural non-toxic;It takes into account inside and outside, is guidance with theory of traditional Chinese medical science, in conjunction with modernization process,
It acts on drug not only and body surface, but also acts on body and deep skin, from inside to outside, exclude the objectionable impurities poison in skin
Element, performance is mild, promotes the metabolism of Skin Cell, and activated cell vigor improves skin oxidation resistance, can change from substrate
Kind skin state;Adhere to that original spot can slowly take off, and whitening skin is glossy using that skin can be made to significantly change;And
Simple process, raw material easily obtain, and have a good application prospect and market prospects.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to conventional strip
Part or according to the normal condition proposed by manufacturer.
Unless otherwise defined, it anticipates known to all professional and scientific terms as used herein and one skilled in the art
Justice is identical.In addition, any method similar to or equal to what is recorded and material can be applied to the method for the present invention.Wen Zhong
The preferred implement methods and materials are for illustrative purposes only.
The preparation of preparation example spot-eliminating beauty treatment soap
Preparation example 1
Formula: 80 grams of lard soap base, 8 grams of Radix Salviae Miltiorrhizae, 7 grams of traditional Chinese drug inspissation filtrate;Wherein, lard soap base component are as follows: lard 100
Gram, 10 grams of potassium hydroxide;Traditional Chinese drug inspissation filtrate raw material components and its dosage are as follows: 10 grams of Rhizoma Chuanxiong, 10 grams of Radix Angelicae Sinensis, 10 grams of turmeric, white
10 grams of fruit.
Preparation process:
(1) it weighs lard to be placed in flask, is heated to 60 DEG C (± 5 DEG C), after lard thawing, hydroxide is added dropwise
Aqueous solutions of potassium stirs and maintains temperature 1 hour, for use.
(2) example in mass ratio, weighs lard soap base and mixes with DANSHEN KELI, 60 DEG C (± 5 DEG C) are heated to, to lard soap base
After melting completely, stirs and maintains temperature 1 hour, uniform lard soap base mixture is made.
(3) load weighted Rhizoma Chuanxiong, Radix Angelicae Sinensis, turmeric, gingko are put into clean casserole, distilled water, cold water is added
It impregnates 60 minutes, is impregnated with medicinal material for principle, amount of water is 8 times of medicinal material amount when first pass decocts, and heating stirring is boiled from high fire
Boiling starts to calculate decocting time after being changed to small fire, decocts 30 minutes, filters out while hot;Cold water is added to being higher than medicine in second time decoction
About 2 centimetres of plane, heating stirring, since be boiled by fire be changed to after small fire calculate decocting time, decoct 20 minutes, filter while hot
Out.Merging filtrate, concentration filtrate to concentration are stand-by after being 45%.
(4) traditional Chinese drug inspissation filtrate obtained in addition step (3) in the lard soap base mixture in step (2), and after
Continuous stirring keeps 65 DEG C of (± 5 DEG C) temperature, and reduced vacuum is evacuated to product in paste, pour into later it is cooling in template, soap
Block.
Preparation example 2
Formula: 80 grams of lard soap base, 8 grams of Radix Salviae Miltiorrhizae, 7 grams of traditional Chinese drug inspissation filtrate;Wherein, lard soap base component are as follows: lard 100
Gram, 10 grams of potassium hydroxide;Traditional Chinese drug inspissation filtrate raw material components and its dosage are as follows: 6 grams of Rhizoma Chuanxiong, 12 grams of Radix Angelicae Sinensis, 6 grams of turmeric, gingko
12 grams.
Preparation process:
(1) it weighs lard to be placed in flask, is heated to 60 DEG C (± 5 DEG C), after lard thawing, hydroxide is added dropwise
Aqueous solutions of potassium stirs and maintains temperature 1 hour, for use.
(2) example in mass ratio, weighs lard soap base and mixes with DANSHEN KELI, 60 DEG C (± 5 DEG C) are heated to, to lard soap base
After melting completely, stirs and maintains temperature 1 hour, uniform lard soap base mixture is made.
(3) load weighted Rhizoma Chuanxiong, Radix Angelicae Sinensis, turmeric, gingko are put into clean casserole, distilled water, cold water is added
It impregnates 60 minutes, is impregnated with medicinal material for principle, amount of water is 8 times of medicinal material amount when first pass decocts, and heating stirring is boiled from high fire
Boiling starts to calculate decocting time after being changed to small fire, decocts 30 minutes, filters out while hot;Cold water is added to being higher than medicine in second time decoction
About 2 centimetres of plane, heating stirring, since be boiled by fire be changed to after small fire calculate decocting time, decoct 20 minutes, filter while hot
Out.Merging filtrate, concentration filtrate to concentration are stand-by after being 45%.
(4) traditional Chinese drug inspissation filtrate obtained in addition step (3) in the lard soap base mixture in step (2), and after
Continuous stirring keeps 65 DEG C of (± 5 DEG C) temperature, and reduced vacuum is evacuated to product in paste, pour into later it is cooling in template, soap
Block.
Preparation example 3
Formula: 80 grams of lard soap base, 8 grams of Radix Salviae Miltiorrhizae, 7 grams of traditional Chinese drug inspissation filtrate;Wherein, lard soap base component are as follows: lard 100
Gram, 10 grams of potassium hydroxide;Traditional Chinese drug inspissation filtrate raw material components and its dosage are as follows: 8 grams of Rhizoma Chuanxiong, 12 grams of Radix Angelicae Sinensis, 8 grams of turmeric, gingko
10 grams.
Preparation process:
(1) it weighs lard to be placed in flask, is heated to 60 DEG C (± 5 DEG C), after lard thawing, hydroxide is added dropwise
Aqueous solutions of potassium stirs and maintains temperature 1 hour, for use.
(2) example in mass ratio, weighs lard soap base and mixes with DANSHEN KELI, 60 DEG C (± 5 DEG C) are heated to, to lard soap base
After melting completely, stirs and maintains temperature 1 hour, uniform lard soap base mixture is made.
(3) load weighted Rhizoma Chuanxiong, Radix Angelicae Sinensis, turmeric, gingko are put into clean casserole, distilled water, cold water is added
It impregnates 60 minutes, is impregnated with medicinal material for principle, amount of water is 8 times of medicinal material amount when first pass decocts, and heating stirring is boiled from high fire
Boiling starts to calculate decocting time after being changed to small fire, decocts 30 minutes, filters out while hot;Cold water is added to being higher than medicine in second time decoction
About 2 centimetres of plane, heating stirring, since be boiled by fire be changed to after small fire calculate decocting time, decoct 20 minutes, filter while hot
Out.Merging filtrate, concentration filtrate to concentration are stand-by after being 45%.
(4) traditional Chinese drug inspissation filtrate obtained in addition step (3) in the lard soap base mixture in step (2), and after
Continuous stirring keeps 65 DEG C of (± 5 DEG C) temperature, and reduced vacuum is evacuated to product in paste, pour into later it is cooling in template, soap
Block.
Preparation example 4
Formula: 70 grams of coconut oil soap base, 7 grams of Radix Salviae Miltiorrhizae, 20 grams of traditional Chinese drug inspissation filtrate;Wherein, coconut oil soap base component are as follows: coconut palm
100 grams of seed oil, 12 grams of potassium hydroxide;Traditional Chinese drug inspissation filtrate component and its dosage are as follows: 10 grams of Rhizoma Chuanxiong, 10 grams of Radix Angelicae Sinensis, 10 grams of turmeric,
10 grams of gingko.
Preparation process:
(1) it weighs coconut oil to be placed in flask, is heated to 60 DEG C (± 5 DEG C), after coconut oil thawing, hydrogen is added dropwise
Aqueous solutions of potassium is aoxidized, stirs and maintains temperature 1 hour, for use.
(2) example in mass ratio, coconut oil soap base is mixed with DANSHEN KELI, 60 DEG C (± 5 DEG C) are heated to, to coconut oil soap
After base melts completely, temperature is stirred and maintained 1 hour, uniform coconut oil soap base mixture is made.
(3) load weighted Rhizoma Chuanxiong, Radix Angelicae Sinensis, turmeric, gingko are put into clean casserole, distilled water, cold water is added
It impregnates 50 minutes, is impregnated with medicinal material for principle, amount of water is 6 times of medicinal material amount when first pass decocts, and heating stirring is boiled from high fire
Boiling starts to calculate decocting time after being changed to small fire, decocts 25 minutes, filters out while hot;Cold water is added to being higher than medicine in second time decoction
About 2 centimetres of plane, heating stirring, since be boiled by fire be changed to after small fire calculate decocting time, decoct 20 minutes, filter while hot
Out.Merging filtrate, concentration filtrate to concentration are stand-by after being 60%.
(4) the traditional Chinese drug inspissation filtrate obtained in step (3) is added in the coconut oil soap base mixture in step (2), and
Continue to stir, keep 65 DEG C of (± 5 DEG C) temperature, reduced vacuum is evacuated to product in paste, pour into later it is cooling in template, soap
Block.
Preparation example 5
Formula: 40 grams of lard soap base, 40 grams of coconut oil soap base, 8 grams of Radix Salviae Miltiorrhizae, 8 grams of traditional Chinese drug inspissation filtrate;Wherein, lard soap
Base component are as follows: 100 grams of lard, 10 grams of potassium hydroxide;Coconut oil soap base component are as follows: 100 grams of coconut oil, 12 grams of potassium hydroxide;In
Filtrate raw material components and its dosage is concentrated in medicine are as follows: 10 grams of Rhizoma Chuanxiong, 10 grams of Radix Angelicae Sinensis, 10 grams of turmeric, 10 grams of gingko.
Preparation process:
(1) lard soap base and cocounut oil soap base are produced respectively: weighing lard and is placed in flask, are heated to 60 DEG C (± 5 DEG C),
After lard thawing, potassium hydroxide aqueous solution is added dropwise, stirs and maintains temperature 1 hour, for use;It weighs coconut oil and is placed in burning
In bottle, 60 DEG C (± 5 DEG C) are heated to, after coconut oil thawing, potassium hydroxide aqueous solution is added dropwise, stirs and maintains temperature
1 hour, for use.
(2) example in mass ratio, lard soap base, coconut oil soap base are mixed with DANSHEN KELI, are heated to 60 DEG C (± 5 DEG C),
After lard soap base, coconut oil soap base melt completely, temperature is stirred and maintained 1 hour, uniform compound soap base mixing is made
Object.
(3) load weighted Rhizoma Chuanxiong, Radix Angelicae Sinensis, turmeric, gingko are put into clean casserole, distilled water, cold water is added
It impregnates 50 minutes, is impregnated with medicinal material for principle, amount of water is 7 times of medicinal material amount when first pass decocts, and heating stirring is boiled from high fire
Boiling starts to calculate decocting time after being changed to small fire, decocts 30 minutes, filters out while hot;Cold water is added to being higher than medicine in second time decoction
About 2 centimetres of plane, heating stirring, since be boiled by fire be changed to after small fire calculate decocting time, decoct 20 minutes, filter while hot
Out.Merging filtrate, concentration filtrate to concentration are stand-by after being 50%.
(4) traditional Chinese drug inspissation filtrate obtained in addition step (3) in the compound soap based mixtures in step (2), and after
Continuous stirring keeps 65 DEG C of (± 5 DEG C) temperature, and reduced vacuum is evacuated to product in paste, pour into later it is cooling in template, soap
Block.
Preparation example 6
Formula: 70 grams of coconut oil soap base, 20 grams of traditional Chinese drug inspissation filtrate;Wherein, coconut oil soap base component are as follows: coconut oil 100
Gram, 12 grams of potassium hydroxide;Traditional Chinese drug inspissation filtrate component and its dosage are as follows: 10 grams of Rhizoma Chuanxiong, 10 grams of Radix Angelicae Sinensis, 10 grams of turmeric, gingko 10
Gram, 7 grams of Radix Salviae Miltiorrhizae.
Preparation process:
(1) it weighs coconut oil to be placed in flask, is heated to 60 DEG C (± 5 DEG C), after coconut oil thawing, hydrogen is added dropwise
Aqueous solutions of potassium is aoxidized, stirs and maintains temperature 1 hour, for use.
(2) load weighted Rhizoma Chuanxiong, Radix Angelicae Sinensis, turmeric, gingko, Radix Salviae Miltiorrhizae are put into clean casserole, distilled water are added,
It cold water soak 50 minutes, is impregnated with medicinal material for principle, amount of water is 6 times of medicinal material amount when first pass decocts, heating stirring, from big
Fire boils be changed to small fire after start calculate decocting time, decoct 25 minutes, filter out while hot;Second time decoction, it is supreme to be added cold water
In about 2 centimetres of medicine plane, heating stirring, since be boiled by fire be changed to after small fire calculate decocting time, decoct 20 minutes, take advantage of
Heat filters out.Merging filtrate, concentration filtrate to concentration are stand-by after being 60%.
(4) the traditional Chinese drug inspissation filtrate obtained in step (3) is added in the coconut oil soap base mixture in step (2), and
Continue to stir, keep 65 DEG C of (± 5 DEG C) temperature, reduced vacuum is evacuated to product in paste, pour into later it is cooling in template, soap
Block.
Experimental example
The color of human skin is related with the type and quantity of pigment present in skin, and melanin influences skin color
It is maximum.Melanin is formed in the melanocyte in epidermal basal portion.Its process is the tyrosine in melanocyte in junket ammonia
DOPA is generated under the action of sour enzyme, DOPA quinone ultimately forms melanin.Melanin transfer is into basal cell, with epidermal cell
Migrate and be brought to epidermis holostrome, the last depigmentation with falling off for keratinocyte.Tyrosinase is the key enzyme of melanin production,
It controls the forming process of melanin, and level of activity plays a major role to the calmness of pigment.
The grouping situation of following embodiment illustrates:
Cell culture fluid, non-inoculating cell is only added in blank group;Negative control group is vaccinated with cell also and joined cell training
Nutrient solution;Arbutin group is to be vaccinated with cell also to joined cell culture fluid, contains various concentration arbutin in cell culture fluid;Medicine
Liquid group is to be vaccinated with cell also to joined cell culture fluid, the medical fluid containing various concentration in cell culture fluid.
It is the DMEM culture solution containing 10% fetal calf serum, cell used that used cell culture fluid, which includes when changing liquid,
For murine melanoma cells B16 (Shanghai cell institute, the Chinese Academy of Sciences), cell used is tested every time and is taken from same passage cell.
Experimental example 1, melanogenesis Inhibition test
The murine melanoma cells B16 for selecting logarithmic growth phase is digested with 0.25% trypsase (Gibco company), is adjusted
Then whole cell concentration is inoculated in culture bottle, in 37 DEG C, 5% CO of volume fraction2Under conditions of cultivate for 24 hours after, negative control
Group is changed liquid 1 time with fresh culture solution (i.e. the DMEM culture solution containing 10% fetal calf serum), and medical fluid group (is derived from system of the invention
It is changed for example 1-3) and arbutin (Sigma company) group with the DMEM culture solution containing 10% fetal calf serum of addition test medicine
Liquid 1 time, arbutin, liquor strength set 10,50,100 μ g/ml, tri- kinds of concentration respectively, and each concentration sets 12 multiple holes;Blank group
Cell only plus with other two groups of identical culture solutions is not added;Each group is placed in 37 DEG C, volume fraction 5%CO2Under the conditions of cultivate
72h harvests cell with 0.25% trypsase, discards supernatant liquid after 1500r/min centrifugation 5min, add PBS buffer solution by cell
Auto-regulating System of Density of Heavy Medium is to 105/ ml or so, each 1ml cell suspension of drawing are placed in 3 parallel tool plug pipes, are centrifuged under 1500r/min
Liquid is discarded supernatant after 5min, 200 μ l distilled water, which are first added, makes cell suspend again, and 1ml ethyl alcohol: ether solution (v/v=is then added
1:1) to dissolve the opaque particle of non-melanocyte, 15min is placed at room temperature, and 5min is centrifuged under 3000r/min and is discarded supernatant
Liquid, is added dimethyl sulfoxide (Sigma company), the 1mol/L NaOH solution of 1ml 10wt%, and water-bath 30min at 80 DEG C makes thin
Born of the same parents' agglomerate is completely dissolved.OD value, wavelength 490nm are surveyed with enzyme mark (thermoelectricity Shanghai Instrument Ltd.) instrument.Wherein, melanin contains
Amount inhibiting rate is calculated according to following formula, and the results are shown in Table 1.
Melanin content inhibiting rate %=[1- (ODMedical fluid groupValue-ODBlank groupValue)/(ODNegative control groupValue-ODBlank groupValue)] × 100%
1 melanogenesis inhibiting rate of table
Compared with negative control group,*P < 0.05, * * p < 0.01;Compared with arbutin group,#P < 0.05,##P <
0.01。
Table 1 the result shows that, traditional Chinese drug inspissation filtrate in preparation example 1-3 of the present invention has the significant work for inhibiting melanogenesis
With, and this inhibiting effect is better than the arbutin of same concentrations, therefore, for may refrain from melanin genesis, with preparation example 1-3
Middle traditional Chinese drug inspissation filtrate can be better than arbutin for whitening as active constituent.
Embodiment 2, tyrosinase inhibition test
Selection grows to the murine melanoma cells B16 of nearly Fusion Strain, is digested with 0.25% pancreatin (Gibco company),
Cell concentration is adjusted, with every hole 5 × 103A to be inoculated in 96 orifice plates, every 150 μ L of boreliquid amount is placed in 37 DEG C, volume fraction is
5%CO2Under the conditions of cultivate for 24 hours after, negative control group changes liquid 1 time with the DMEM culture solution containing 10% fetal calf serum, medical fluid group
Liquid 1 time is changed with the DMEM culture solution containing 10% fetal calf serum of addition test medicine with arbutin (Sigma company) group, bear
Fruit glycosides, liquor strength set 10,50 and 100 μ g/ml, tri- kinds of concentration respectively, and each concentration sets 12 multiple holes;Blank group only adds and contains
There is the DMEM culture solution of 10% fetal calf serum that cell is not added;Each group is placed in 37 DEG C, volume fraction 5%CO2Under the conditions of cultivate
72h, incline culture solution, is washed twice with PBS, adds 90 hole μ L/ TritonX-100 (Sigma company), shakes 30min, is added
0.2% levodopa solution (Sigma company), 60 hole μ L/, 37 DEG C of incubation 20min survey OD value with microplate reader, and wavelength is
490nm.Wherein, inhibitory activity against tyrosinase calculates according to the following formula, and the results are shown in Table 2:
Inhibitory activity against tyrosinase %=[1- (ODMedical fluid groupValue-ODBlank groupValue)/(ODNegative control groupValue-ODBlank groupValue)] ×
100%
2 inhibitory activity against tyrosinase of table
Compared with negative control group,*P < 0.05, * * p < 0.01;Compared with arbutin group,#P < 0.05,##P <
0.01。
Table 2 the result shows that, there is significant tyrosinase to inhibit to live for traditional Chinese drug inspissation filtrate in preparation example 1-3 of the present invention
Property, and this inhibiting effect is better than the arbutin of same concentrations, therefore, for may refrain from tyrosinase activity, with preparation
Traditional Chinese drug inspissation filtrate can be better than arbutin for whitening as active constituent in example 1-3.
Embodiment 3, cell growth inhibition assay
Selection grows to the murine melanoma cells B16 (Shanghai cell institute, the Chinese Academy of Sciences) of nearly Fusion Strain, with 0.25% pancreas
Enzyme (Gibco company) digestion, adjusts cell concentration, with every hole 5 × 103A to be inoculated in 96 orifice plates, every 150 μ L of boreliquid amount is set
In 37 DEG C, volume fraction 5%CO2Under the conditions of cultivate for 24 hours after, negative control group with containing 10% fetal calf serum DMEM train
Nutrient solution (Gibco company) is changed liquid 1 time, and medical fluid group and arbutin group are with addition test medicine containing 10% fetal calf serum
DMEM culture solution (Gibco company) changes liquid 1 time, and arbutin, liquor strength set 10,50,100 μ g/ml, tri- concentration respectively,
Each concentration sets 12 multiple holes;Blank group only adds identical culture solution that cell is not added;Each group is placed in 37 DEG C, volume fraction 5%
CO2Under the conditions of cultivate 72h, MTT (Gibco company) 20 hole μ L/ is added in 4h before terminating, discard supernatant liquid after 37 DEG C of incubation 4h,
Dimethyl sulfoxide (Sigma company) 150 hole μ L/, oscillation 10min or so is added.With microplate reader (the limited public affairs of thermoelectricity Shanghai instrument
Department) survey OD value, wavelength 490nm.Wherein, cell proliferation inhibition rate is calculated by following formula, and the results are shown in Table 3:
Cell proliferation inhibition rate %=[1- (ODMedical fluid groupValue-ODBlank groupValue)/(ODNegative control groupValue-ODBlank groupValue)] × 100%
3 Melanoma Cell Proliferation inhibiting rate of table
Compared with negative control group,*P < 0.05, * * p < 0.01;Compared with arbutin group,#P < 0.05,##P <
0.01。
Table 3 the result shows that, the traditional Chinese drug inspissation filtrate in preparation example 1-3 of the present invention acts on quite compared with arbutin, does not have
Significant statistical difference, and cytotoxic effect is faint.
Experimental example 4, cutaneous pigmentation improve experiment
Animal subject is albino guinea-pig, half male and half female, weight 350-380g, purchased from Shandong University's Experimental Animal Center.Take 8
Cavy shaves off the hair at its back, and circle takes the position of 5 pieces of 2 × 2cm, and 5 positions are exposed under ultraviolet light irradiation for several times,
So that every guinea pig back generates 5 pigmentation positions, it is labeled as 1,2,3,4,5.After model construction success, the exposed skin of cavy
The sensitivity phenomenons such as decortication, redness are had no at skin.To avoid hair growth from influencing observation in test process, once every three days take off
Hair.
After being exposed to the irradiation of last ultraviolet light 11 days, fixed respectively using the washing of beauty soap described in preparation example 1,4,5,6
Pigmentation position 1,2,3,4, and wash pigmentation position 5 using clear water, daily set time, each cleaning was primary sooner or later,
Continue 25 days, and respectively after the 14th and 20 day, using Scheffe multiple comparison graph, compares application preparation example 1,4-6 beauty soap
One of each pigmentation position and application control (clear water) position brightness, be according to the judgment criteria for being listed in table 4
Comparison result classification.
4 brightness evaluation criterion table of table
Using after preceding and application after 14 days, 20 days, 5 judge personnel to three pigmentation positions of cavy 1,2,3,4,
Compared with the pigmentation position 5 of application control, and compared result is classified.It is obtained from everyone of five evaluation personnels
The total score of the vision rating fraction at each pigmentation position is defined as the vision rating fraction of every kind of beauty soap.According to eight
Only coloured cavy calculates the average visual ranking score of every kind of handmade soap.The Δ value of vision ranking score by respectively using preparation example 1,
The vision ranking score that vision ranking score behind beauty soap 14 days of 4-6 subtracts before application is calculated.Wherein, Δ value height shows to change
It is good to be apt to pigmented effect.The results are shown in Table 5.
5 pigmentation effect Δ value table of table
The result shows that beauty soap of the invention can improve pigmentation, and with using the time to increase, improvement
It gets a promotion.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
Specific implementation of the invention is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, exist
Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to of the invention
Protection scope.