CN109431902A - A kind of preparation method and applications of Cactus Flower extract - Google Patents

Abstract

The invention discloses a kind of preparation method and applications of Cactus Flower extract.This method comprises the following steps: (1) crushing Cactus Flower, obtain Cactus Flower powder；(2) it obtains that solvent is added in Cactus Flower powder in step (1), is extracted under the conditions of 0~100 DEG C, obtain extracting solution；Then extracting solution is separated by solid-liquid separation, collects liquid；The liquid concentration that will be collected into again obtains Cactus Flower extract.Extracting method of the invention is easy to operate, the Cactus Flower extract of acquisition has moisturizing water lock, moistens brilliant white skin, the effect of falling off for promoting keratoderma, can be applied in daily chemical products or external preparation for skin product, has a vast market foreground in moisturizing, whitening, anti-ageing class product.

Description

A kind of preparation method and applications of Cactus Flower extract

Technical field

The invention belongs to technical field of extraction of Chinese traditional medicine, in particular to the preparation method of a kind of Cactus Flower extract and its answer With.

Background technique

Cactus is Cactaceae opuntia, and the whole world is there are about 70~110 categories, more than 2000.Have in China There is long medicinal history.Supplementary Amplifications of the Compendium of Materia Medica is recorded: cactus is lightly seasoned cold in nature, the function promoting flow of qi and blood circulation, alleviating pain and detumescence, heat-clearing solution Poison.Civil, people often treated with cactus suffer from stomachache long, perianal abscess is rushed down blood, bronchial asthma, palpitation and insomnia, burn, snakeworm and is stung The illnesss such as wound, chronic rotten inflammation, canker.Modern medical research has found that cactus have clearing heat and detoxicating, antibacterial anti-inflammatory, it is hypoglycemic, Reducing blood lipid, blood pressure reduction effect and other effects, root and stem protein rich in, minerals, cellulose and vitamin C etc., together When the various actives functional component such as triterpene, malic acid, succinic acid, alkaloid, flavonoids and mucopolysaccharide rich in, these Ingredient is all the active matter for having special role to skin, has the ability removed and inhibit free radical, is particularly suitable in beauty It is applied in cosmetics.

Existing research report is concentrated mainly on cactus fleshy root and stem portion, and for colored research almost without. Cactus Flower is exceedingly beautiful gorgeous, flower yellow, and the general 5~10cm of diameter, Dan Sheng or several grows thickly in inclined scape top edge, loudspeaker Shape is oval, and colored edge also has some thorns or seta, likes sunlight, and it is strong to be resistant to arid ability.Someone is from celestial being It is isorhamnetin 3-O- acacia that the palm, which is spent middle separation, identified to the ingredient that steroid 5α-reductase has obvious inhibitory activity, 2 hydrate of disaccharide glycosides, and inquired into its improvement result to hypertrophy of the prostate, the results showed that, pair in Cactus Flower extract Hypertrophy of the prostate improves significantly.But in cosmetics and other external application fields, about the research of Cactus Flower, almost No.

Therefore, it is highly desirable further to study Cactus Flower and develop into the product with application value.

Summary of the invention

The primary purpose of the present invention is that the shortcomings that overcoming the prior art and insufficient, a kind of Cactus Flower extract is provided Preparation method.

Another object of the present invention is to provide the preparation methods for the Cactus Flower extract that the method is prepared.

A further object of the present invention is to provide the applications of the preparation method of the Cactus Flower extract.

The purpose of the invention is achieved by the following technical solution: a kind of preparation method of Cactus Flower extract, including such as Lower step:

(1) Cactus Flower is crushed, obtains Cactus Flower powder；

(2) it obtains that solvent is added in Cactus Flower powder in step (1), extracts, obtain under the conditions of 0~100 DEG C To extracting solution；Then extracting solution is separated by solid-liquid separation, collects liquid；The liquid concentration that will be collected into again obtains Cactus Flower extraction Object.

Cactus Flower described in step (1) preferably turn round thorn Cactus Flower, great Hua Cactus Flower, nopal spend and One or more of flat peach Cactus Flower.

The granular size of Cactus Flower powder described in step (1) is 20~70 mesh.

The mass ratio of Cactus Flower powder described in step (2) and solvent is 1:1~40；Preferably 1:3~40.

Solvent described in step (2) is one of water and lower alcohol or two or more mixtures；Preferably water, second Alcohol, propylene glycol, one of butanediol and glycerol or two or more mixtures；More preferably water, concentration are 60% (w/w's) Ethanol water, concentration are the butanediol aqueous solution of 20% (w/w), and concentration is the glycerine water solution or fourth two of 10% (w/w) Alcohol, propylene glycol and water are the mixed solvent that 1:1:2 is mixed to get by weight.

Normal pressure and temperature extraction, ultrasonic extraction, heating extraction or low temperature is extracted as described in step (2) to extract.

The low temperature is extracted as 60 DEG C or less low temperature and extracts；More preferably 10~60 DEG C of low temperature extract.

The condition of the ultrasonic extraction is preferred are as follows: 30 DEG C of temperature, frequency 35KHz.

The temperature of extraction described in step (2) is preferably 60 DEG C or less；More preferably 10~60 DEG C.

The time of extraction described in step (2) is 60~180min.

Separation of solid and liquid described in step (2) is that (the removal dregs of a decoction) are separated by solid-liquid separation by the way of centrifugation.

The centrifugation is to be centrifuged using tripod pendulum type batch centrifugal, cup type centrifuge or tab (s) formula centrifuge；Preferably adopt With the tripod pendulum type batch centrifugal for being mounted with 300~700 mesh filter cloth bags, cup type centrifuge or tab (s) formula centrifuge are centrifuged.

The speed of the centrifugation is 2000~20000rpm.

The preparation method of the Cactus Flower extract further includes further separating the liquid collected in step (2) The step of purifying.

Described is isolated and purified to be isolated and purified by the way of UF membrane；Miillpore filter is preferably used, and/or Ultrafiltration membrane is isolated and purified.

The miillpore filter is preferably ceramic micro filter film.

The aperture of the miillpore filter is 100~1000nm；Preferably 500~800nm.

The aperture of the ultrafiltration membrane is 1000~100000Da；Preferably 5000Da

Concentration described in step (2) is that the relative density under the conditions of being concentrated into 25 DEG C is 1.010~1.080；Preferably 1.020~1.070.

Concentration described in step (2) is preferably concentrated by the way of reverse osmosis membrane or reduced pressure.

The reverse osmosis membrane is preferably polysulphone reverse osmosis film.

The preparation method of the Cactus Flower extract further includes by Cactus Flower extract obtained in step (2) The step of solid Cactus Flower extract is made is further dried；Specifically: by Cactus Flower extract obtained in step (2) Crushed after being dried, sieving, obtains solid Cactus Flower extract.

The sieving was 80 meshes.

A kind of Cactus Flower extract, is prepared by method described in any of the above embodiments.

Application of the Cactus Flower extract in daily chemical products or external preparation for skin product.

The daily chemical products are preferably cosmetics, including moisturizing, moisturizing, whitening, exfoliating and the makeup of anti-aging class Product, such as cream emulsion, film powder class, thermophilic curry class, aerosol class and muffin class etc..

The additive amount of the Cactus Flower extract accounts for the 1~50% of daily chemical products weight；Preferably 5~20%.

The external preparation for skin product includes external preparation for skin drug etc..

A kind of Cactus Flower skin-moisturizing, skin gel, the component including following weight percent content:

The molecular weight of the hyaluronic acid is preferably 1,200,000.

The preparation method of the Cactus Flower skin-moisturizing, skin gel, includes the following steps:

(1) it is swollen sufficiently in the water of 10 times of volumes carbomer dispersion, obtain gel；

(2) methylparaben, propylben and Phenoxyethanol are added in glycerol, are heated to 80 DEG C and stir evenly, Obtain mixed liquor A；

(3) hyaluronic acid is dissolved into remaining water, adds Radix et Caulis Opuntiae Dillenii extract, obtain mixed liquid B；

(4) gel, mixed liquor A and mixed liquid B are uniformly mixed, obtain Cactus Flower skin-moisturizing, skin gel.

The time of stirring described in step (2) is preferably 30min.

A kind of Cactus Flower is dispelled cutin skin lotion, the component including following weight percent content:

A phase:

B phase:

Triethanolamine 0.40%；

Polyacrylamide 0.30%；

Above-mentioned Cactus Flower extract 5~20.00%；

C phase:

The B phase also contains essence.

The essence is preferably lemon extract.

The additive amount of the essence preferably accounts for the Cactus Flower and dispels the 0.1% of cutin skin lotion weight.

The additive amount of the Cactus Flower extract preferably accounts for the Cactus Flower and dispels cutin skin lotion weight 20.00%.

The Cactus Flower is dispelled the preparation method of cutin skin lotion, is included the following steps:

(I) 75~80 DEG C are heated to after mixing the component in A phase, each component in C phase is then added, is stirred It is even, obtain mixed material；

(II) mixed material obtained in step (I) is cooled to 45 DEG C, adds the component in B phase, stir evenly, obtains To Cactus Flower extract.

The condition of stirring described in step (I) are as follows: 75~80 DEG C, 20000r/min stirring 20min.

The present invention has the following advantages and effects with respect to the prior art:

1, the present invention provides a kind of preparation method and applications of Cactus Flower extract, extract and divide through 60 DEG C or less low temperature From Cactus Flower extract after purification, there is higher active polysaccharide content, further includes after extracting obtained extracting solution progress Processing: separation, purifying and concentration.It also can also be the further concentration of said extracted liquid at solid powder.Through external biochemical It tests, BALB/C-Nu/Nu- nude mice-adhesive tape removing horny layer of epidermis method test, human body application test and other effects is it is demonstrated experimentally that originally Invention extract have moisturizing water lock, moisten brilliant white skin, promote the falling off effect of keratoderma, applied to moisturizing, whitening, Anti-ageing class product has a vast market foreground.

2, extracting method of the present invention is easy to operate, and obtained Cactus Flower extract can be applied to outside daily chemical products or skin With in product, especially there is apparent advantage in moisturizing, whitening, anti-ageing class product.

Detailed description of the invention

Fig. 1 is (w/w) Cactus Flower extract and 10% (w/w) Sodium Hyaluronate 10% under different relative humidities Moisture absorption in 4 hours, (abscissa is corresponding in turn to water lock effect picture from left to right in figure: white vitriol, ammonium sulfate, chlorination Sodium, ammonium nitrate, frerrous chloride, calcium nitrate, zinc nitrate, calcium chloride, potassium acetate, zinc chloride).

Fig. 2 is (w/w) Cactus Flower extract and 10% (w/w) Sodium Hyaluronate 10% under different relative humidities Moisture absorption in 8 hours, (abscissa is corresponding in turn to water lock effect picture from left to right in figure: white vitriol, ammonium sulfate, chlorination Sodium, ammonium nitrate, frerrous chloride, calcium nitrate, zinc nitrate, calcium chloride, potassium acetate, zinc chloride).

Fig. 3 is (w/w) Cactus Flower extract and 10% (w/w) Sodium Hyaluronate 10% under different relative humidities Moisture absorption in 24 hours, (abscissa is corresponding in turn to water lock effect picture from left to right in figure: white vitriol, ammonium sulfate, chlorination Sodium, ammonium nitrate, frerrous chloride, calcium nitrate, zinc nitrate, calcium chloride, potassium acetate, zinc chloride).

Fig. 4 is (w/w) Cactus Flower extract and 10% (w/w) Sodium Hyaluronate 10% under different relative humidities Moisture absorption in 48 hours, (abscissa is corresponding in turn to water lock effect picture from left to right in figure: white vitriol, ammonium sulfate, chlorination Sodium, ammonium nitrate, frerrous chloride, calcium nitrate, zinc nitrate, calcium chloride, potassium acetate, zinc chloride).

Fig. 5 is the Cactus Flower extract that each embodiment obtains and comparative example extract at 25 DEG C, relative humidity 76% Moistening effect figure under part.

Fig. 6 is that under the conditions of relative humidity 50% ± 5%, it is solidifying not smear Cactus Flower skin-moisturizing, skin at 25 DEG C ± 1 DEG C of temperature The dry skin figure of glue.

Fig. 7 is under the conditions of relative humidity 50% ± 5%, to smear Cactus Flower skin-moisturizing, skin gel at 25 DEG C ± 1 DEG C of temperature 8 hours skin effects figures afterwards.

Fig. 8 is that using experimental group, (skin-moisturizing, skin of the extract containing Cactus Flower is solidifying with moisture of skin tester detection human body It is prepared by glue-embodiment 7), control group [be formulated same experimental group, unique difference is Cactus Flower extract to replace with 5% (w/ W) Sodium Hyaluronate (1,000,000 molecular weight)], (bare substrate is formulated same experimental group to matrix group, and unique difference is cactus Flower extract remove) and blank group (any cosmetics not being smeared, using water as tested material) after in 1~8 hour inner skin water Divide content results figure.

Specific embodiment

Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto. Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus；Unless It illustrates, agents useful for same and raw material of the present invention can pass through commercially available acquisition.

Embodiment 1

It takes 15kg to turn round thorn Cactus Flower and is ground into 20 mesh powders, the pure water of 300kg is added into powder, stirs evenly, Be placed in heating extraction device, under the conditions of 50 DEG C heat preservation extract 120min, with load 300 mesh filter cloth bags tripod pendulum type batch centrifugal, in It is centrifugated extracting solution under the conditions of 4000rpm, is then concentrated under reduced pressure into the phase of concentrate under the conditions of 60 DEG C with dual-effect concentrator It is 1.05 (25 DEG C) to get Cactus Flower extract to density.

Embodiment 2

It takes 100 grams of torsion thorn Cactus Flowers to be ground into 40 mesh powders, the pure water of 1000g is added into powder, stirs evenly, It is placed in supersonic extractors, extracts 40min under the conditions of 30 DEG C of temperature, frequency 35KHz, obtain extracting solution.By said extracted liquid It is separated by solid-liquid separation, centrifugal speed 3000rpm, is centrifuged 30 minutes with cup type centrifuge, collect centrifugate.Then by centrifugate In 55 DEG C of progress vacuum-concentrcteds, until it is dry, dry concentrate is crushed, powder crosses 80 mesh screens, obtains the extraction of solid Cactus Flower Object.

Embodiment 3

1kg great Hua Cactus Flower is taken to be ground into 70 mesh powders；60% (w/w) ethyl alcohol that 20kg is added into powder is water-soluble Liquid stirs evenly, and is placed in refluxing extraction tank, 60 DEG C of refluxing extraction 60min, and filtering obtains extracting solution.By said extracted liquid pump Enter supercentrifuge to be separated by solid-liquid separation, centrifugal speed 15000rpm, collects separating liquid.Then centrifugate progress vacuum is subtracted Pressure concentration, the relative density for being concentrated under reduced pressure into concentrate are 1.040 (25 DEG C) to get Cactus Flower extract.

Embodiment 4

It takes 10kg nopal's pollen to be broken into 50 mesh powders, 20% (w/w) butanediol of 30kg is added into powder Aqueous solution stirs evenly, and is placed in extractor, and 100min is extracted in stirring under the conditions of 40 DEG C of temperature, obtains extracting solution.It will be above-mentioned Extracting solution is separated by solid-liquid separation with tab (s) formula centrifuge, centrifugal speed 5000rpm, collects centrifugate.By above-mentioned centrifugation liquid pump Enter membrane separation system, with the miillpore filter of 0.8um and the ultrafiltration membrane continuous processing of 5000Da, obtains UF membrane liquid.It will be upper It states UF membrane liquid and carries out vacuum-concentrcted, the relative density for being concentrated under reduced pressure into concentrate is 1.020 (25 DEG C) to get cactus Flower extract.

Embodiment 5

It takes 1kg flat peach Cactus Flower to be ground into 40 mesh powders, 10% glycerol/pure water (W/ of 40kg is added into powder W) (weight ratio of glycerol and pure water is 1:9), stirs evenly, is placed in heating extraction device, keep the temperature and extract under the conditions of 50 DEG C 180min is centrifugated extracting solution with the tripod pendulum type batch centrifugal for loading 500 mesh filter cloth bags under the conditions of 4000rpm, then uses 500nm The ceramic micro filter UF membrane and polysulphone reverse osmosis film in aperture are concentrated into 5 times that concentrate is medicinal material amount, and relative density is at this time 1.025 (25 DEG C) are to get Cactus Flower extract.

Embodiment 6

Take 50kg great Hua Cactus Flower to be ground into 30 mesh powders, into powder be added 250kg butanediol/propylene glycol/ Pure water (weight ratio=1:1:2), stirs evenly, is placed in heating extraction device, 100min is extracted under the conditions of 10 DEG C, with loading The tripod pendulum type batch centrifugal of 700 mesh filter cloth bags is centrifugated extracting solution under the conditions of 4000rpm, then micro- with the ceramics in the aperture 800nm Filter membrane and 5000Da Ultra filtration membrane centrifugate are finally 1.070 (25 with the relative density that reverse osmosis membrane is concentrated into concentrate DEG C) to get Cactus Flower extract.

Comparative example

With embodiment 3, difference is that raw materials used is great Hua cactus, remaining operation is identical.

1kg great Hua cactus powder is taken to be broken into 70 mesh powders；60% (w/w) ethyl alcohol that 20kg is added into powder is water-soluble Liquid stirs evenly, and is placed in refluxing extraction tank, 60 DEG C of refluxing extraction 60min, and filtering obtains extracting solution.By said extracted liquid pump Enter supercentrifuge to be separated by solid-liquid separation, centrifugal speed 15000rpm, collects separating liquid.Then centrifugate progress vacuum is subtracted Pressure concentration, the relative density for being concentrated under reduced pressure into concentrate are 1.040 (25 DEG C) to get Radix et Caulis Opuntiae Dillenii extract.

Embodiment 7

A kind of Cactus Flower skin-moisturizing, skin gel, is made of the raw material of following weight percent content:

Wherein, the molecular weight of hyaluronic acid is 1,200,000.

A kind of preparation method of Cactus Flower skin-moisturizing, skin gel, its step are as follows:

(1) carbomer is taken to be scattered in the water of 10 times of volumes, gel is made in sufficiently swelling；

(2) methylparaben, propylben and Phenoxyethanol, 80 DEG C of agitating and heating 30min are dissolved by heating with glycerol；

(3) it after remaining distilled water dissolution hyaluronic acid, adds Radix et Caulis Opuntiae Dillenii extract and dissolves；

(4) by (1) (2) (3) mix, stir evenly to get.

Wherein Cactus Flower extract is preferably the Radix et Caulis Opuntiae Dillenii extract prepared in embodiment 2.

Embodiment 8

A kind of Cactus Flower is dispelled cutin skin lotion, is made of the raw material of following weight percent content:

A phase:

B phase:

C phase:

A kind of preparation method of Cactus Flower skin-moisturizing, skin cream, its step are as follows:

(1) A phase and C phase are weighed, is separately heated to 75~80 DEG C.

(2) A is added to C phase, stirs evenly homogeneous (75~80 DEG C, 20000r/min) 20min.

(3) it is cooled to 45 DEG C while stirring, B phase is added, stirs evenly.To obtain the final product.

Wherein Cactus Flower extract is preferably the Radix et Caulis Opuntiae Dillenii extract prepared in embodiment 5.

The experiment of 9 moisture-keeping efficacy of embodiment

1. material and instrument

Material:

Trier: the Cactus Flower extract (10%, W/W) prepared in embodiment 1；

Control group: HA (Sodium Hyaluronate, 1,000,000 molecular weight, 10%, W/W)；

Trier and reference material are diluted to required concentration with pure water.

Reagent: white vitriol, ammonium sulfate, sodium chloride, ammonium nitrate, frerrous chloride, calcium nitrate, zinc nitrate, calcium chloride, vinegar Sour potassium, zinc chloride are that analysis is pure, and saturated solution is made in pure water.

Instrument: electronic balance, drier, Hygrothermograph, electric drying oven with forced convection

2. experimental method

Moisture absorption/moisturizing experiment: precision weighs the above-mentioned Cactus Flower extract sample several pieces of equivalent, and diameter 3cm is added Weighing bottle in, every part of sample is individually positioned in the drier of following saturated solution: white vitriol (25 DEG C, it is relatively wet Spend RH=90%), ammonium sulfate (25 DEG C, relative humidity RH=81%), sodium chloride (25 DEG C, relative humidity RH=76%), nitric acid Ammonium (25 DEG C, relative humidity RH=67%), frerrous chloride (25 DEG C, relative humidity RH=56%), calcium nitrate (25 DEG C, it is relatively wet Spend RH=50%), zinc nitrate (25 DEG C, relative humidity RH=42%), calcium chloride (25 DEG C, relative humidity RH=31%), acetic acid Potassium (25 DEG C, relative humidity RH=20%), zinc chloride (25 DEG C, relative humidity RH=10%).Standing time is 4,8,24, 48h, quality (W2) after weighing quality (W1) before sample is placed respectively and placing.

It calculates according to the following formula: (W2-W1)/W1 × 100%.

3. result and analysis

The result is shown in Figure 1~4, in different relative humidity, sample moisture absorption/moistening effect and the relationship of time are proportional Relationship.As the result is shown in 67% or more relative humidity, Cactus Flower extract shows moisture absorption effect, and wettability power is better than same The Sodium Hyaluronate of isoconcentration；Relative humidity 56% and it is following when, Cactus Flower extract shows water lock effect, i.e. moisture content Loss is slower, and effect is better than the Sodium Hyaluronate of comparable sodium.

4. each embodiment moistening effect verifying

In saturated sodium chloride solution (25 DEG C, relative humidity RH=76%), it is small that each embodiment 24 is carried out according to the above method When moistening effect verifying, it is specific as follows:

2 sample of embodiment is diluted to 20% (W/W) concentration with pure water, measures relative density to dilute again after 1.06 (25 DEG C) 10% (w/w) carries out moistening effect confirmatory experiment.

Embodiment 1,3~6 and comparative example are diluted to 10% (W/W) concentration with pure water, carry out moisturizing after pure water dilution Compliance test result experiment.

As a result as shown in figure 5, as can be seen from Figure 5, under the conditions of 25 DEG C, relative humidity 76%, embodiment 1-5 extract is equal With preferable moistening effect, there is significant difference with the moistening effect of comparative example extract.

10 human efficiency's verifying of embodiment-moisturizing

Human test's moisturizing compound moisture-keeping efficacy

1. material and instrument

Experimental group: Cactus Flower skin-moisturizing, skin gel prepared by embodiment 7.

Control group: formula is Cactus Flower extract to be replaced with 5% (w/w) hyalomitome with embodiment 7, unique difference Sour sodium (1,000,000 molecular weight).

Matrix group: bare substrate, formula with embodiment 7, unique difference are Cactus Flower extract to remove.

Blank group: not smearing any cosmetics, using water as tested material.

Test equipment: moisture of skin tester (Corneometer), product type: CM825.

2. test method

Volunteer: subject amounts to 8 people, and 18 years old~45 years old；Without serious systemic disease, without immune deficiency or autoimmune Disease patient；No active anaphylactia person；Previously to skin-care cosmetics without severe allergy person；It was not used in nearly 1 month Hormone medicine and immunosuppressor person；Other clinical test persons are not participated in；Use according to regulations tested material and all information；Test Preceding all volunteers should fill in informed consent form.

Test index: the probe of moisture of skin tester is that the principle based on capacitor measures.Its reading value is bigger, water Divide content bigger；Conversely, moisture content is smaller.

Environment: constant-temperature constant-humidity environment is tested, temperature: 25 DEG C ± 1 DEG C, relative humidity 50% ± 5%.

Data acquisition: subject in designated environment after 20~30min, on the inside of left and right arm it is selective go out 4 3cm × 3cm test area, it is interregional every 1cm.Experimental group and blank, matrix, control group are randomly dispersed in left and right arm.Use skin Skin moisture tester carries out the measurement in tested region and each control zone, and each region is successively measured in parallel 3 times, is averaged. The blank value of each test zone is first measured, 1mg ± 0.1mg sample/cm is then pressed²Dosage, try out latex finger cot will test produce Product are spread evenly across in trial zone.After smearing, when measuring 1h, 2h, 3h, 4h, 5h, 6h, 7h and 8h respectively, tested region and blank The moisture content of skin of control zone.(being measured when verifying by this time).The test of the same volunteer is by the same measurement personnel It completes.

3. result and analysis

It is shown in Table 1 and Fig. 6~8.Illustrate to have used the skin-moisturizing, skin gel of Cactus Flower extract can increase considerably skin Water content is better than the Sodium Hyaluronate of same additive amount to the moisture holding capacity of skin.Fig. 7 explanation is applied to be extracted containing Cactus Flower The skin-moisturizing, skin gel of object has the effect of making skin profit, slides, is bright.

1 human skin determination of moisture result of table

Time (hour)	Experimental group (%)	Control group (%)	Matrix group (%)	Blank group (%)
					1	37.1±0.6	38.3±0.4	30.0±0.6	24.1±0.7
2	35.4±0.5	31.1±0.6	28.2±0.3	23.2±0.4
					3	32.3±0.5	28.7±0.5	26.1±0.4	22.2±0.5
4	31.1±0.3	27.5±0.5	25.3±0.3	21.5±0.3
					5	29.7±0.7	26.6±0.7	23.3±0.2	20.3±0.4
6	28.3±0.5	25.3±0.8	22.5±0.5	20.6±0.5
					7	26.2±0.2	23.4±0.9	21.3±0.1	19.7±0.6
8	26.5±0.4	21.6±0.4	20.1±0.6	18.0±0.9

11 efficacy validations of embodiment-exfoliating

1. material

Experimental group: Cactus Flower prepared by embodiment 8 is dispelled cutin skin lotion.

Control group: pure water.

Animal subject: SPF grades of nude mices of BALB/C-Nu/Nu (male female fifty-fifty, Guangdong Medical Lab Animal Center provides).

2. experimentation

Skin thickness measurement: taking nude mice, and after the neck that breaks is put to death, with operating scissors clip skin of back, then careful rejecting is subcutaneous Musculature obtains in vitro full skin.Between the isolated skin handled well is placed in glass slide, with the thickness of vernier caliper measurement skin Degree remembers A.

Destratum corneum skin: horny layer of epidermis method is removed by adhesive tape, nude mice four limbs are fixed first, are uniformly pasted with sealed adhesive tape On the skin of destratum corneum to be removed, adhesive tape is then thrown off, by being operated under skin thickness mensuration item after operating 30 times repeatedly, is surveyed Skin thickness after determining destratum corneum, is denoted as B.

The measurement of destratum corneum ability: taking cotton swab (oneself inhale saturated sample) to smear nude mice skin of back 10 seconds repeatedly, and one It by operating under skin thickness mensuration item after continuously smearing seven days, measures test group skin thickness, is denoted as C twice.

3 results and calculating:

Sample destratum corneum ability is calculated as follows:

[(A-B)-(A-C)]/(A-B) × 100%.

As a result such as table 2.For the continuous smearing of display after 7 days, test group has preferable removal corneocyte ability.

2 nude mice epidermis exfoliating result of table

12 polyoses content detection method of embodiment and result

1, the preparation of reference substance solution: taking DEXTROSE ANHYDROUS reference substance 10mg, accurately weighed, sets in the molten measuring bottle of 100ml, adds Enter pure water dissolution, be diluted to scale, shakes up to get (containing DEXTROSE ANHYDROUS 0.1mg) in every lml.

2, the preparation of standard curve:

(1) accurate to measure reference substance solution 0ml, 0.1ml, 0.3ml, 0.5ml, 0.7ml, 0.9ml, tool plug test tube is set respectively In, respectively plus water is mended to 2.0ml；

(2) then 5% (w/v) phenol solution lml is added in each precision, shakes up, accurate rapidly that the concentrated sulfuric acid (98%) is added 5ml shakes up, and places 10 minutes, sets in 45 DEG C of water-baths and keep the temperature 20 minutes, takes out, is rapidly cooled to room temperature, with the examination of 0ml reference substance Pipe is blank, and according to UV-VIS spectrophotometry, absorbance is measured at the wavelength of 490nm, dense using absorbance as ordinate Degree is abscissa, draws standard curve.

3, sample measures: precision measures the Cactus Flower extract liquid prepared in the embodiment of the present invention 1~6 and comparative example 5ml (solution that embodiment 2 adds pure water to be configured to 20% (w/w) concentration carries out analysis detection), add diethyl ether 100ml, is heated to reflux 1 Hour, it stands, lets cool, carefully discard ether solution, residue sets water-bath Back stroke and uses up ether.80% (w/w) ethanol solution is added 100ml is heated to reflux 1 hour, filters while hot, and filter residue and filter are washed by several times with 80% (w/w) ethanol solution 30ml of heat, filter residue It is set in flask together with filter paper, adds water 150ml, be heated to reflux 2 hours.It filters while hot, with a small amount of hot water washing nozzle, merging filtrate It with washing lotion, letting cool, moves in 250ml measuring bottle, be diluted with water to scale, appropriate dilution, precision measure dilution lml after shaking up, It sets in tool plug test tube, adds water 1.0ml, obtain sample to be tested；Then by the method for the preparation of sample to be tested reference standard curve (step Rapid 2 (2)), measure absorbance, from standard curve read test solution in the weight (mg) containing glucose, calculate to get.

The determination of polysaccharide result of the Cactus Flower extract of the present invention of table 3

The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention, It should be equivalent substitute mode, be included within the scope of the present invention.

Claims (10)

1. a kind of preparation method of Cactus Flower extract, which comprises the steps of:

(1) Cactus Flower is crushed, obtains Cactus Flower powder；

(2) it obtains that solvent is added in Cactus Flower powder in step (1), extracts, mentioned under the conditions of 0~100 DEG C Take liquid；Then extracting solution is separated by solid-liquid separation, collects liquid；The liquid concentration that will be collected into again obtains Cactus Flower extract.

2. the preparation method of Cactus Flower extract according to claim 1, it is characterised in that:

Cactus Flower described in step (1) is to turn round thorn Cactus Flower, and great Hua Cactus Flower, nopal spends and flat peach celestial being One or more of palm flower；

Solvent described in step (2) is one of water and lower alcohol or two or more mixtures.

3. the preparation method of Cactus Flower extract according to claim 1, it is characterised in that:

The mass ratio of Cactus Flower powder described in step (2) and solvent is 1:1~40；

Solvent described in step (2) is water, ethyl alcohol, propylene glycol, one of butanediol and glycerol or two or more mixing Object.

4. the preparation method of Cactus Flower extract according to claim 1, it is characterised in that:

The granular size of Cactus Flower powder described in step (1) is 20~70 mesh.

The temperature of extraction described in step (2) is 10~60 DEG C；

The time of extraction described in step (2) is 60~180min；

Separation of solid and liquid described in step (2) is to be separated by solid-liquid separation by the way of centrifugation；Wherein, the centrifugation is to use It is mounted with the tripod pendulum type batch centrifugal of 300~700 mesh filter cloth bags, cup type centrifuge or tab (s) formula centrifuge are centrifuged, centrifugation Speed is 2000~20000rpm；

Concentration described in step (2) is to be concentrated by the way of reverse osmosis membrane or reduced pressure；Wherein, the reverse osmosis Permeable membrane is polysulphone reverse osmosis film；

Concentration described in step (2) is that the relative density under the conditions of being concentrated into 25 DEG C is 1.010~1.080.

5. the preparation method of Cactus Flower extract according to claim 1, it is characterised in that: further include by step (2) The step of liquid of middle collection further isolates and purifies；

Described is isolated and purified to be isolated and purified using miillpore filter and/or ultrafiltration membrane；

The miillpore filter is ceramic micro filter film；

The aperture of the miillpore filter is 100~1000nm；

The aperture of the ultrafiltration membrane is 1000~100000Da.

6. the preparation method of Cactus Flower extract according to claim 1, it is characterised in that: further include by step (2) Obtained in Cactus Flower extract the step of solid Cactus Flower extract is made is further dried；Specifically: by step (2) Obtained in Cactus Flower extract crushed after being dried, sieving, obtain solid Cactus Flower extract.

7. a kind of Cactus Flower extract, it is characterised in that: be prepared by the described in any item methods of claim 1~6.

8. Cactus Flower extract as claimed in claim 7 existing in daily chemical products or external preparation for skin product using its feature In:

The daily chemical products are cosmetics；

The external preparation for skin product is external preparation for skin drug.

9. a kind of Cactus Flower skin-moisturizing, skin gel, it is characterised in that: the component including following weight percent content:

The preparation method of the Cactus Flower skin-moisturizing, skin gel, includes the following steps:

(1) it is swollen sufficiently in the water of 10 times of volumes carbomer dispersion, obtain gel；

(2) methylparaben, propylben and Phenoxyethanol are added in glycerol, are heated to 80 DEG C and stir evenly, obtains Mixed liquor A；

(3) hyaluronic acid is dissolved into remaining water, adds Radix et Caulis Opuntiae Dillenii extract, obtain mixed liquid B；

(4) gel, mixed liquor A and mixed liquid B are uniformly mixed, obtain Cactus Flower skin-moisturizing, skin gel.

A kind of cutin skin lotion 10. Cactus Flower is dispelled, it is characterised in that: the component including following weight percent content:

A phase:

B phase:

Triethanolamine 0.40%；

Polyacrylamide 0.30%；

Cactus Flower extract 5~20.00% as claimed in claim 7；

C phase:

The B phase also contains essence；

The additive amount of the essence is to account for the Cactus Flower to dispel the 0.1% of cutin skin lotion weight；

The Cactus Flower is dispelled the preparation method of cutin skin lotion, is included the following steps:

(I) 75~80 DEG C are heated to after mixing the component in A phase, each component in C phase is then added, is uniformly mixed, Obtain mixed material；

(II) mixed material obtained in step (I) is cooled to 45 DEG C, adds the component in B phase, stir evenly, obtains celestial being People slaps flower extract；

The condition of stirring described in step (I) are as follows: 75~80 DEG C, 20000r/min stirring 20min.