CN109430636A - A kind of production method of Cordyceps militaris fermented beverage - Google Patents

A kind of production method of Cordyceps militaris fermented beverage Download PDF

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Publication number
CN109430636A
CN109430636A CN201811037408.0A CN201811037408A CN109430636A CN 109430636 A CN109430636 A CN 109430636A CN 201811037408 A CN201811037408 A CN 201811037408A CN 109430636 A CN109430636 A CN 109430636A
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CN
China
Prior art keywords
cordyceps militaris
cordyceps
fermented beverage
pupa
slurry
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Withdrawn
Application number
CN201811037408.0A
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Chinese (zh)
Inventor
吕庆茂
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Harbin Weiping Technology Development Co Ltd
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Harbin Weiping Technology Development Co Ltd
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Priority to CN201811037408.0A priority Critical patent/CN109430636A/en
Publication of CN109430636A publication Critical patent/CN109430636A/en
Withdrawn legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention belongs to food processing technology fields, and in particular to a kind of production method of Cordyceps militaris fermented beverage.Weigh the degreased pupa powder of certain mass, clear water and neutral proteinase is added, pupa albumen hydrolyzate is filtered to obtain after hydrolysis, mulberries pulp and pupa albumen hydrolyzate are uniformly mixed, sterilized, cooling, Cordyceps militaris is fermented to obtain Cordyceps militaris fermentation liquid, Cordyceps militaris fermentation liquid is obtained into Cordyceps militaris slurry with mycelium colloid mill defibrination, Cordyceps militaris slurry is got product using filter, allotment, filling, sealing, sterilizing.Not only the smell of fruits is very sweet for Cordyceps militaris fermented beverage of the invention, but also is rich in cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide and flavones and other functional ingredient, often drinks, good health-care effect can be played to human body.

Description

A kind of production method of Cordyceps militaris fermented beverage
Technical field
The invention belongs to food processing technology fields, and in particular to a kind of production method of Cordyceps militaris fermented beverage.
Background technique
Cordyceps militaris is also Cordceps militaris or northern Chinese caterpillar Fungus, belongs to Eumycota, Ascomycetes, Hypocreales, Clavicipitaceae, worm Grass belongs to, Cordceps militaris carbohydrate rich in, protein, amino acid and more than 30 kinds of needed by human body microelement, greatly Quantity research confirms that cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide and other functional ingredient and the natural cordyceps contained in Cordyceps militaris are close, In the most Ideal Substitute that field of health care food is natural cordyceps, Cordyceps Resources are rare, expensive, are difficult to become Raw materials for production of health food, and the Cordyceps militaris price of natural Cordyceps militaris and cultivation is also higher, and it is edible when taste it is bad, it is difficult So that consumer generally receives, fermentation liquid containing cordyceps mycelium and cordyceps sinensis and pupa worm that artificial liquid state fermentation obtains Grass seed entity chemical composition, pharmacological action are almost the same, can replace natural cs, so the pupa worm obtained with liquid deep layer fermenting Careless mycelium and fermentation liquid are raw material, are processed into cordycepin beverage, not only greatly reduce production cost, also big at a low price for exploitation Crowd's worm grass product of pupating has opened up new thinking.
Summary of the invention
The object of the present invention is to provide a kind of production sides of Cordyceps militaris fermented beverage with health-care efficacy that the smell of fruits is very sweet Method.
The object of the present invention is achieved like this:
Potato is cleaned peeling, the 180-220 grams of potato chips for being cut into 4-5 millimeters thick is taken, adds 1000 milliliters of water, boil half an hour After filter to get filtrate, 20g glucose is added in filtrate, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, stirring and dissolving is simultaneously settled to It 1000 milliliters, sterilizes 15-20 minutes at 121 DEG C, is cooled to 24-26 DEG C and obtains Cordyceps militaris seed culture medium, from inclined-plane Cordyceps militaris spawn is inoculated with into Cordyceps militaris seed culture medium, shaking table culture under conditions of 24-26 DEG C, 160-200rpm Obtain Cordyceps militaris seed within 50-60 hours.
The degreased pupa powder of certain mass is weighed, the clear water of 10-12 times of quality is added, by every gram of degreased pupa powder 150- Neutral proteinase is added in the ratio of 200 units, and pupa albumen hydrolysis is filtered to obtain after hydrolyzing 2-3 hours under the conditions of 45-50 DEG C Liquid.
Mulberries are broken into mulberries pulp with beater, according to the ratio of mass ratio 1:1 by mulberries pulp and pupa albumen water It solves liquid to be uniformly mixed, sterilize 15-20 minutes under the conditions of 85-90 DEG C, be cooled to 24-26 DEG C and obtain Cordyceps militaris fermented and cultured Base accesses 10% Cordyceps militaris seed, in 24-26 DEG C, the condition of 160-200rpm in Cordyceps militaris fermentation medium Obtain Cordyceps militaris fermentation liquid within lower shaking table culture 90-100 hours.
Cordyceps militaris fermentation liquid is obtained into Cordyceps militaris slurry with mycelium colloid mill defibrination, Cordyceps militaris is starched using filter, allotment, filling Dress, sealing, sterilizing are got product.
Cordyceps militaris fermented beverage beneficial effect of the invention includes the following aspects:
First, cordycepin, cordycepic acid and the Cordyceps sinensis polysaccharide that Cordyceps militaris fermented beverage of the invention is generated rich in Cordyceps militaris metabolism, warp The content for crossing cordycepin in the Cordyceps militaris fermented beverage of the measurement embodiment of the present invention is 21.3 milligrams every liter, and the content of cordycepic acid is 3.25 grams every liter, the content of Cordyceps sinensis polysaccharide is 1.93 grams every liter.Cordycepin, cordycepic acid and Cordyceps sinensis polysaccharide are cordyceps sinensis and pupa worm The main composition of grass has and adjusts the multiple efficacies such as function of immune system, antifatigue.
Second, Cordyceps militaris fermented beverage of the invention aglycone-type flavones rich in contains flavonoids abundant in mulberries Compound, but these flavone compound majorities and sugar are combined into the form of glucosides, these glucoside type flavones can be with obtected pupa worm The beta-glucosidase that grass metabolism generates is converted to aglycone-type flavones, and aglycone-type flavones is higher than glucoside type flavonoid active, raw Reason activity is stronger.By measurement, mulberries flavone glycoside is converted to flavone aglycone in the Cordyceps militaris fermented beverage of the embodiment of the present invention Conversion ratio be 90%.
Third, Cordyceps militaris fermented beverage of the invention have strong mulberries fragrance, and mulberries pulp passes through Cordyceps militaris spawn After fermentation, the reference state aroma-producing substance in mulberries can be converted to free by the beta-glucosidase that Cordyceps militaris spawn metabolism generates State aroma-producing substance increases the fragrance of Cordyceps militaris fermented beverage of the present invention.
Specific embodiment
The Cordyceps militaris CICC14013 that the present invention uses is purchased in Chinese industrial Microbiological Culture Collection administrative center.
Potato is cleaned peeling, 200 grams of potato chips for being cut into 4-5 millimeters thick is taken, adds 1000 milliliters of water, boil half an hour After filter to get filtrate, 20g glucose is added in filtrate, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, stirring and dissolving is simultaneously settled to It 1000 milliliters, sterilizes 15 minutes at 121 DEG C, is cooled to 25 DEG C and obtains Cordyceps militaris seed culture medium, pupa is inoculated with from inclined-plane Cordyceps species obtain Cordyceps militaris in shaking table culture 55 hours under conditions of 25 DEG C, 180rpm into Cordyceps militaris seed culture medium Liquid seeds.
The clear water of 11 times of quality is added in the degreased pupa powder for weighing certain mass, by 180 units of every gram of degreased pupa powder Ratio be added neutral proteinase, filter to obtain pupa albumen hydrolyzate after hydrolyzing 2.5 hours under the conditions of 48 DEG C.
Mulberries are broken into mulberries pulp with beater, according to the ratio of mass ratio 1:1 by mulberries pulp and pupa albumen water It solves liquid to be uniformly mixed, sterilize 15 minutes under the conditions of 88 DEG C, be cooled to 25 DEG C and obtain Cordyceps militaris fermentation medium, in Cordyceps militaris The Cordyceps militaris seed of access 10% in liquid fermentation medium obtains for shaking table culture 96 hours under conditions of 25 DEG C, 180rpm Cordyceps militaris fermentation liquid.
Cordyceps militaris fermentation liquid is obtained into Cordyceps militaris slurry with mycelium colloid mill defibrination, Cordyceps militaris is starched using filter, allotment, filling Dress, sealing, sterilizing are got product.

Claims (1)

1. a kind of production method of Cordyceps militaris fermented beverage, it is characterized in that being prepared by following process:
(1) potato is cleaned peeling, take the 180-220 grams of potato chips for being cut into 4-5 millimeters thick, add 1000 milliliters of water, it is small to boil half When after filter to get filtrate, 20g glucose is added in filtrate, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, stirring and dissolving is simultaneously settled to It 1000 milliliters, sterilizes 15-20 minutes at 121 DEG C, is cooled to 24-26 DEG C and obtains Cordyceps militaris seed culture medium, from inclined-plane Cordyceps militaris spawn is inoculated with into Cordyceps militaris seed culture medium, shaking table culture under conditions of 24-26 DEG C, 160-200rpm Obtain Cordyceps militaris seed within 50-60 hours;
(2) the degreased pupa powder of certain mass is weighed, the clear water of 10-12 times of quality is added, by every gram of degreased pupa powder 150-200 Neutral proteinase is added in the ratio of a unit, filters to obtain pupa albumen hydrolyzate after hydrolyzing 2-3 hours under the conditions of 45-50 DEG C;
(3) mulberries are broken into mulberries pulp with beater, hydrolyze mulberries pulp and pupa albumen according to the ratio of mass ratio 1:1 Liquid is uniformly mixed, and is sterilized 15-20 minutes under the conditions of 85-90 DEG C, is cooled to 24-26 DEG C and is obtained Cordyceps militaris fermentation medium, The Cordyceps militaris seed of access 10% in Cordyceps militaris fermentation medium, under conditions of 24-26 DEG C, 160-200rpm Obtain Cordyceps militaris fermentation liquid within shaking table culture 90-100 hours;
By Cordyceps militaris fermentation liquid with mycelium colloid mill defibrination obtain Cordyceps militaris slurry, Cordyceps militaris slurry using filter, allotment, it is filling, Sealing, sterilizing are got product.
CN201811037408.0A 2018-09-06 2018-09-06 A kind of production method of Cordyceps militaris fermented beverage Withdrawn CN109430636A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110771876A (en) * 2019-11-05 2020-02-11 质每(中国)有限公司 Method for fermenting ginseng by cordyceps militaris

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105995362A (en) * 2016-06-29 2016-10-12 黄秀英 Biological cordyceps-sinensis culturing beverage with mulberry juice as carriers
CN106852426A (en) * 2017-01-02 2017-06-16 哈尔滨伟平科技开发有限公司 A kind of preparation method of the banana skin beverage with hypotensive activity
CN107460223A (en) * 2017-08-31 2017-12-12 江苏科技大学 A kind of degreasing silkworm chrysalis hydrolysate for microculture and its preparation method and application

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105995362A (en) * 2016-06-29 2016-10-12 黄秀英 Biological cordyceps-sinensis culturing beverage with mulberry juice as carriers
CN106852426A (en) * 2017-01-02 2017-06-16 哈尔滨伟平科技开发有限公司 A kind of preparation method of the banana skin beverage with hypotensive activity
CN107460223A (en) * 2017-08-31 2017-12-12 江苏科技大学 A kind of degreasing silkworm chrysalis hydrolysate for microculture and its preparation method and application

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110771876A (en) * 2019-11-05 2020-02-11 质每(中国)有限公司 Method for fermenting ginseng by cordyceps militaris

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Application publication date: 20190308

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