CN109430469A - A kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function - Google Patents
A kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
Abstract
The invention discloses a kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function, belongs to health product technology field.The dark green tea is made of 20-40 parts of birch young pilose antler, 60-80 parts of tealeaves, 6-10 parts of cassia seed, 6-8 parts of chrysanthemum, 3-5 parts of the coptis, 5-9 parts of radix bardanae of raw material.The present invention utilizes scavenging effect and antioxidation of the active polysaccharide substance to free radical in birch young pilose antler, dark green tea is fermented tea making together with birch young pilose antler, cassia seed, chrysanthemum, the coptis and radix bardanae, it is sufficiently mixed it, polysaccharide, peroxidase and the collaboration of flavonoid active substance play a role, it removes interior free yl and feeds antioxidant, it is obvious to pancreas function repairing effect.The present invention is different from birch young pilose antler class medicament, remains the original flavor of dark green tea, enriches the health-care efficacy of dark green tea, and preparation method is simple, at low cost, easy to spread.
Description
Technical field
The present invention relates to a kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function, belong to health care product and its
Preparation technical field.
Background technique
Birch young pilose antler is also known as Inonotus obliquus, belongs to Eumycota, Hymenomycetes, without brown Zoopagales, Polyporaceae, fine hole Pseudomonas.Birch is fine and soft
For the bracket fungus of brown, it is born under the bark of silver birch, silky oak etc. more.Mainly originate in Northern Europe of north latitude 40-50 degree, Russia
Siberia, the Far East Area, and Hokkaido area of Japan, Heilongjiang Province of China and Jilin Province Changbaishan area.The brown hole of birch
Bacterium has strengthen immunity, antitumor, antiviral, anti-oxidant, anti-inflammatory, antibody Monoclonal and the multiple biological activities effect such as hypoglycemic.
Inonotus obliquus contains polysaccharide, triterpene compound, dish acyl glutamic acid, fragrant oxalic acid, syringic acid DE isoreactivity substance.
And studying discovery Fuscoporia obliqua polysaccharide has the function of stronger removing activation oxygen radical, plays antioxidation (Hui Jing, Song
The Antioxidation in vitro of Ya Na, Sun Xiujuan etc., Fuscoporia obliqua polysaccharide study [J], edible mushroom journal, 2006,13 (2): 29-
31).The researchs such as MA show that Fuscoporia obliqua polysaccharide has stronger ferric iron back, anti-grease matter, antioxidation and removes DPPH certainly
By ability (MAL S, CHENH X, ZHU W C, the et al.Effect of different drying methods on of base
physicochemical properties and antioxidant activities of polysaccharides
Extracted from mushroom Inonotus obliquus [J] .Food ResInt, 2011,50 (2): 633-640).
Therefore, birch young pilose antler is widely used in field of health care products, develops Inonotus obliquus additive in succession, health drink, medicinal tea with
And the series of products such as injection.Pancreas, important one of the organ of human body, the pancreatic juice of secretion play " main during food digestion
The effect at angle ".Make the chronic pancreas in China since environmental quality such as declines, smokes, drinks at the unsound life style in recent years
Scorching disease incidence increases year by year.Free radical adjust oxidative damage be chronic pancreatitis occur the main reason for, radicals scavenging and
Antioxidant supply has a certain effect treating chronic pancreatitis.Therefore using birch young pilose antler as active material repairing pancreas
Function has feasibility.Qu Juanjuan etc. describes application of the Fuscoporia obliqua polysaccharide component in prevention and treatment chronic pancreatitis, and (China is specially
Sharp Authorization Notice No. CN105147717B), but its effective component is single, without other auxiliary materials, uses mainly as drug, function
Single, mouthfeel is not good enough.It is therefore desirable to develop it is a kind of not only can with repairing pancreas function, but also do not lose dark green tea peculiar flavour health care it is black
Tea.
Summary of the invention
The present invention provides a kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function, belongs to health care product production
Technical field.
The technical scheme adopted by the invention is that:
A kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function of the present invention, is by following parts by weight
Raw material be prepared: 20-40 parts of birch young pilose antler, 60-80 parts of tealeaves, 6-10 parts of cassia seed, 6-8 parts of chrysanthemum, 3-5 parts of the coptis and ox
5-9 parts of burdock root.
The preparation method of birch young pilose antler dark green tea of the present invention with repairing pancreas function, comprising the following steps:
(1) choose by weight without rot, the blocky birch of mildew it is fine and soft, cleaning, which is placed in dryer, to be dried to moisture and contains
Amount is in 5-8%；
(2) the birch young pilose antler after step (1) drying is placed in pulverizer and is crushed, smashed birch young pilose antler is crossed into 16 meshes,
Obtain birch young pilose antler particle；
(3) pure water, water-bath under the conditions of 60 DEG C is added according to solid-liquid ratio 1:40 in birch young pilose antler particle obtained by step (2)
Then 2.5h uses 0.2 μm of filter filtering supernatant, and supernatant is concentrated into original under the conditions of 60 DEG C using Rotary Evaporators
The 30% of volume,
Chloroform and n-butanol are configured to Sevage reagent according to volume ratio for 5 to 1, then filtrate and Sevage reagent are pressed
It is mixed according to volume ratio 5 to 1, vibrates 20min deproteinized, centrifuging and taking supernatant repeats addition Sevage reagent to no white precipitate and analyses
Until out；It is by volume again 1 to 4 mixing, 4 DEG C of alcohol precipitation 12h, then mixed liquor is set by 95% concentration ethanol of supernatant
15000rpm is centrifuged 10min, is finally freeze-dried sediment and obtains birch young pilose antler extract；
(4) by cassia seed, chrysanthemum, the coptis and radix bardanae is selected, cleaning, it is dry be 5-8% to water content after, be placed in powder
It is crushed in broken machine, smashes it through 16 meshes, obtain cassia seed, chrysanthemum, the coptis, radix bardanae hybrid particles；
(5) by cassia seed, chrysanthemum, the coptis and radix bardanae particle obtained by birch young pilose antler extract, step (4) obtained by step (3)
It is uniformly mixed with the tea mixing after finishing, just rubbing, then carries out pile-fermentation, room temperature control is 25-30 DEG C, temperature control in heap
For system at 45-55 DEG C, humid control 80-90%, time control is 24-36h；
(6) the tealeaves mixture that pile-fermentation is completed is rubbed again, controls the time in 6-8min, carried out after the completion of rubbing again
It is pressed into brick tea using mold after dry, baking, obtains finished product after metering packing.
Chronic pancreatitis is a kind of long-term irreversible pancreatic disease, the disease incidence of past chronic pancreatitis is lower than ten thousand/
One, but unsound life style increases its disease incidence year by year.Heavy drinking, environmental factor and inherent cause can
It can be the main reason for causing chronic pancreatitis disease incidence to increase.However, there is presently no effective methods to treat chronic pancreas
It is scorching.Operative treatment can only delay disease and slow down pain.The traditional remedies such as diet control and enzyme method of substitution take time and effort and imitate
Fruit is unobvious.The oxidative damage that free radical is adjusted is the main reason for chronic pancreatitis occurs.And radicals scavenging and anti-oxidant
Substance supply has a certain effect repairing pancreas function.The substances such as flavonoids, polysaccharide can act as remove free radical and
Oxidation resistant effect.Dark green tea of the present invention is cooked major ingredient, selects birch young pilose antler, cassia seed, chrysanthemum, radix bardanae to do auxiliary material, wherein being provided with
It removes free radical and oxidation resistant effect, each material analyzing is as follows:
Dark green tea (dark tea) because the appearance of made tea is in black, therefore is gained the name.Dark green tea belongs to one of six big teas, after category
Fermented tea, main producing region are the ground such as Sichuan, Yunnan, Hubei, Hunan, Shaanxi, Anhui.Not only polyphenoils rich in dark green tea
Matter such as catechin, Tea Pigment, flavonoids, vitamin C, vitamin E, mono- carrotene of D etc., and contain a large amount of tool antioxygen
The microelement such as zinc, manganese, copper (constitution element of SOD) and selenium (constitution element of GSHPX) etc. of change effect.Catechu in dark green tea
The more complicated flavonoids etc. of element, theaflavin, theanine and tea polysaccharide, especially content all has the function of removing free radical,
Thus has the function of anti-oxidant, delaying cell aging.
Containing having more than 200 various active substances in inonotus obliquus sclerotium, most important one polysaccharide component it is verified that
There are very strong reduction and removing DPPH free radical, hydroxyl radical free radical, the free radicals such as superoxide anion and hydrogen peroxide in vitro
Oxidation resistance.
Flavonoid substances content in cassia seed, chrysanthemum is very high (> 24mg/g), they are to DPPH free radical scavenging ability
(> 70%) is than the radish seed (5.53%) of low flavones content, Siraitia grosvenorii (47.40%), Radix Glycyrrhizae (55.80%), brush-cherry seed
(19.12%), semen ziziphi spinosae (19.23%) is stronger.
Contain peroxidase in radix bardanae, it can enhance the vigor of cellular immune mechanism, remove internal oxygen radical, hinder
The only generation and accumulation of lipofuscin pigment in vivo, anti-aging provide the interior shield of confrontation and scavenging activated oxygen for body
Environment.
The coptis, alias: taste connects, river connects, Rhizoma Coptidis, belongs to Ranunculaceae, Coptis herbaceos perennial, and phyllopodium is hesitated, hard paper
Matter, ovate triangle, three totally cleave, central sliver ovate diamond shape, pinniform drastic crack, and there is sharp sawtooth at edge, and side life sliver not equal 2 is deep
It splits；The long 5-12cm of petiole.There is the effect of heat-clearing and damp-drying drug, purging fire for removing toxin.Have the function of hypoglycemic effect, reducing blood lipid simultaneously.
Beneficial effects of the present invention: the birch young pilose antler dark green tea and its preparation that the present invention provides a kind of with repairing pancreas function
Method by dark green tea and birch young pilose antler, is determined using scavenging effect and antioxidation of the active polysaccharide substance to free radical in birch young pilose antler
Pine torch, chrysanthemum, the coptis and radix bardanae ferment tea making together, are sufficiently mixed it, and polysaccharide, peroxidase and flavones are living
Property substance collaboration play a role, remove interior free yl simultaneously feed antioxidant, it is obvious to pancreas function repairing effect.This hair
It is bright to be different from birch young pilose antler class medicament, the original flavor of dark green tea is remained, the health-care efficacy of dark green tea, and preparation method letter are enriched
It is single, it is at low cost, it is easy to spread.
Specific embodiment
Technical scheme is described further combined with specific embodiments below, but not limited to this.
Embodiment 1
A kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function of the present invention, is by following parts by weight
Raw material be prepared: birch young pilose antler 20 parts, 60 parts of tealeaves, 6 parts of cassia seed, 6 parts of chrysanthemum, 3 parts of the coptis, 5 parts of radix bardanae.
The preparation method of birch young pilose antler dark green tea of the present invention with repairing pancreas function, comprising the following steps:
(1) choose by weight without rot, the blocky birch of mildew it is fine and soft, cleaning, which is placed in dryer, to be dried to moisture and contains
Amount is 5%；
(2) the birch young pilose antler after step (1) drying is placed in pulverizer and is crushed, smashed birch young pilose antler is crossed into 16 meshes,
Obtain birch young pilose antler particle；
(3) pure water, water-bath under the conditions of 60 DEG C is added according to solid-liquid ratio 1:40 in birch young pilose antler particle obtained by step (2)
Then 2.5h uses 0.2 μm of filter filtering supernatant, and supernatant is concentrated into original under the conditions of 60 DEG C using Rotary Evaporators
The 30% of volume,
Chloroform and n-butanol are configured to Sevage reagent according to volume ratio for 5 to 1, then filtrate and Sevage reagent are pressed
It is mixed according to volume ratio 5 to 1, vibrates 20min deproteinized, centrifuging and taking supernatant repeats addition Sevage reagent to no white precipitate and is precipitated
Until；It is by volume again 1 to 4 mixing, 4 DEG C of alcohol precipitation 12h, then mixed liquor is set into 15000rpm by 95% concentration ethanol of supernatant
It is centrifuged 10min, finally sediment is freeze-dried and obtains birch young pilose antler extract；
(4) by cassia seed, chrysanthemum, the coptis and radix bardanae is selected, cleaning, it is dry be 5% to water content after, be placed in crushing
It is crushed in machine, smashes it through 16 meshes, obtain cassia seed, chrysanthemum, the coptis, radix bardanae hybrid particles；
(5) cassia seed, chrysanthemum, the coptis and radix bardanae obtained by birch young pilose antler extract, step (4) obtained by step (3) are extracted
Object is uniformly mixed with the tea mixing after finishing, just rubbing, and then carries out pile-fermentation, and room temperature control is 25 DEG C, temperature control in heap
System is at 45 DEG C, and humid control 80%, the time, control is for 24 hours；
(6) the tealeaves mixture that pile-fermentation is completed is rubbed again, controls the time in 6min, is done after the completion of rubbing again
It is pressed into brick tea using mold after dry, baking, obtains finished product after metering packing.
Embodiment 2
A kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function of the present invention, is by following parts by weight
Raw material be prepared: birch young pilose antler 30 parts, 70 parts of tealeaves, 8 parts of cassia seed, 7 parts of chrysanthemum, 4 parts of the coptis, 7 parts of radix bardanae.
The preparation method of birch young pilose antler dark green tea of the present invention with repairing pancreas function, comprising the following steps:
(1) choose by weight without rot, the blocky birch of mildew it is fine and soft, cleaning, which is placed in dryer, to be dried to moisture and contains
Amount is 7%；
(2) the birch young pilose antler after step (1) drying is placed in pulverizer and is crushed, smashed birch young pilose antler is crossed into 16 meshes,
Obtain birch young pilose antler particle；
(3) pure water, water-bath under the conditions of 60 DEG C is added according to solid-liquid ratio 1:40 in birch young pilose antler particle obtained by step (2)
Then 2.5h uses 0.2 μm of filter filtering supernatant, and supernatant is concentrated into original under the conditions of 60 DEG C using Rotary Evaporators
The 30% of volume,
Chloroform and n-butanol are configured to Sevage reagent according to volume ratio for 5 to 1, then filtrate and Sevage reagent are pressed
It is mixed according to volume ratio 5 to 1, vibrates 20min deproteinized, centrifuging and taking supernatant repeats addition Sevage reagent to no white precipitate and is precipitated
Until；It is by volume again 1 to 4 mixing, 4 DEG C of alcohol precipitation 12h, then mixed liquor is set into 15000rpm by 95% concentration ethanol of supernatant
It is centrifuged 10min, finally sediment is freeze-dried and obtains birch young pilose antler extract；
(4) by cassia seed, chrysanthemum, the coptis and radix bardanae is selected, cleaning, it is dry be 5-8% to water content after, be placed in powder
It is crushed in broken machine, smashes it through 16 meshes, obtain cassia seed, chrysanthemum, the coptis, radix bardanae hybrid particles；
(5) cassia seed, chrysanthemum, the coptis and radix bardanae obtained by birch young pilose antler extract, step (4) obtained by step (3) are extracted
Object is uniformly mixed with the tea mixing after finishing, just rubbing, and then carries out pile-fermentation, and room temperature control is 28 DEG C, temperature control in heap
For system at 50 DEG C, humid control 85%, time control is 30h；
(6) the tealeaves mixture that pile-fermentation is completed is rubbed again, controls the time in 7min, is done after the completion of rubbing again
It is pressed into brick tea using mold after dry, baking, obtains finished product after metering packing.
Embodiment 3
A kind of birch young pilose antler dark green tea and preparation method thereof with repairing pancreas function of the present invention, is by following parts by weight
Raw material be prepared: birch young pilose antler 40 parts, 80 parts of tealeaves, 10 parts of cassia seed, 8 parts of chrysanthemum, 5 parts of the coptis, 9 parts of radix bardanae.
The preparation method of birch young pilose antler dark green tea of the present invention with repairing pancreas function, comprising the following steps:
(1) choose by weight without rot, the blocky birch of mildew it is fine and soft, cleaning, which is placed in dryer, to be dried to moisture and contains
Amount is 8%；
(2) the birch young pilose antler after step (1) drying is placed in pulverizer and is crushed, smashed birch young pilose antler is crossed into 16 meshes,
Obtain birch young pilose antler particle；
(3) pure water, water-bath under the conditions of 60 DEG C is added according to solid-liquid ratio 1:40 in birch young pilose antler particle obtained by step (2)
Then 2.5h uses 0.2 μm of filter filtering supernatant, and supernatant is concentrated into original under the conditions of 60 DEG C using Rotary Evaporators
The 30% of volume,
Chloroform and n-butanol are configured to Sevage reagent according to volume ratio for 5 to 1, then filtrate and Sevage reagent are pressed
It is mixed according to volume ratio 5 to 1, vibrates 20min deproteinized, centrifuging and taking supernatant repeats addition Sevage reagent to no white precipitate and is precipitated
Until；It is by volume again 1 to 4 mixing, 4 DEG C of alcohol precipitation 12h, then mixed liquor is set into 15000rpm by 95% concentration ethanol of supernatant
It is centrifuged 10min, finally sediment is freeze-dried and obtains birch young pilose antler extract；
(4) by cassia seed, chrysanthemum, the coptis and radix bardanae is selected, cleaning, it is dry be 8% to water content after, be placed in crushing
It is crushed in machine, smashes it through 16 meshes, obtain cassia seed, chrysanthemum, the coptis, radix bardanae hybrid particles；
(5) cassia seed, chrysanthemum, the coptis and radix bardanae obtained by birch young pilose antler extract, step (4) obtained by step (3) are extracted
Object is uniformly mixed with the tea mixing after finishing, just rubbing, and then carries out pile-fermentation, and room temperature control is 30 DEG C, temperature control in heap
For system at 55 DEG C, humid control 90%, time control is 36h；
(6) the tealeaves mixture that pile-fermentation is completed is rubbed again, controls the time in 8min, is done after the completion of rubbing again
It is pressed into brick tea using mold after dry, baking, obtains finished product after metering packing.
Comparative example 1
Comparative example 1 prepares dark green tea according to the method for the embodiment of the present invention 1, but the difference is that does not add birch in its composition of raw materials
Fine and soft ingredient is set, other are all identical.
Comparative example 2
Comparative example 2 prepares birch young pilose antler dark green tea according to the method for the embodiment of the present invention 1, but the difference is that in its composition of raw materials not
The ingredient of radix bardanae is added, other are all identical.
Comparative example 3
Comparative example 4 prepares birch young pilose antler dark green tea according to the method for the embodiment of the present invention 1, but the difference is that in its composition of raw materials not
The ingredient of cassia seed is added, other are all identical.
Comparative example 4
Comparative example 4 prepares birch young pilose antler dark green tea according to the method for the embodiment of the present invention 1, but the difference is that in its composition of raw materials not
The ingredient of chrysanthemum is added, other are all identical.
Comparative example 5
Comparative example 5 prepares birch young pilose antler dark green tea according to the method for the embodiment of the present invention 1, but the difference is that in its composition of raw materials not
The ingredient of the coptis is added, other are all identical.
Test example
Animal experiment
(1) test material
Choose 180-220g healthy male rat 80 (Fukang Biotechnology Co., Ltd, Beijing China, family name:
Wistar, week old: 3 weeks or less), dichloro dibutyl ester (DBTC) is purchased from Sigma-Aldrich company
(2) test method
Grouping: healthy rat is divided to 8 groups, every group 10 at random
Feeding method: 8 groups of rat tail veins are injected into DBTC solution (7mg/kg).Simultaneously from the 2nd day, first group is given
1 gained birch young pilose antler dark green tea water of the rat embodiment of the present invention is fed, 2-6 group rat, gives comparative example 1, comparative example 2, right
Ratio 3, comparative example 4 and 5 gained dark green tea water of comparative example are fed, and the 7th group of rat tail vein injects identical same volume
The physiological saline that DBTC lytic agent (ethyl alcohol, glycerol, dimethyl sulfoxide (ratio 1: 2: 2)), the 8th group of rat give equal proportion carries out
It feeds.It is continuous to feed 30 days.
After (3) 30 days, rat physiological status is observed, and completely cut rat pancreas, 10% formalin solution is solid
It is fixed, it is used for histological observation
Test result
After rat tail vein injects DBTC solution, start within the 2nd day to occur that tired sleeping, few dynamic, appetite is reduced, dorsal body setae is fluffy loses
There is just dilute, crissum pollution, have the fidgets sometimes, weight gain is slow in gloss etc., partial rat.First group of rat (feeds
Support 1 gained birch young pilose antler dark green tea water of embodiment) above-mentioned symptom is mitigated after feeding 15 days, and specific experiment the results are shown in Table 1:
The variation of 1 rat body weight of table
| Group | Feeding patterns | Before test | After test |
| 1 | DBTC+ embodiment 1 | 200.2±5.1 | 310.5±15.2 |
| 2 | DBTC+ comparative example 1 | 201.3±5.2 | 230.9±16.0 |
| 3 | DBTC+ comparative example 2 | 200.5±5.6 | 228.9±15.3 |
| 4 | DBTC+ comparative example 3 | 202.0±5.3 | 231.9±15.0 |
| 5 | DBTC+ comparative example 4 | 200.9±5.5 | 225.9±16.3 |
| 6 | DBTC+ comparative example 5 | 200.5±5.3 | 230.5±16.5 |
| 7 | DBTC+DBTC lytic agent | 200.5±5.1 | 305.2±16.0 |
| 8 | DBTC+ physiological saline | 201.3±5.2 | 220.9±14.3 |
After 30 days, the complete pancreatic tissue of 8 groups of rats is taken to carry out structure observation, it has been observed that, 2-6 group and the 8th group
Rat pancreas there is different degrees of atrophy, it is rough surface, irregular, and appearance present it is red or greyish white
There is different degrees of expansion in color, choledochus.And the 1st group and the 7th group of pancreatic tissue are presented that white, appearance be full, surface light
Sliding, choledochus and ductus pancreaticus are evenly distributed, and two groups of pancreatic tissues are without significant difference.As it can be seen that present invention gained birch young pilose antler dark green tea has
Certain pancreas function repair, and cooperate between birch young pilose antler, cassia seed, chrysanthemum and radix bardanae in formula, it cooperates with
Effect is played, it is indispensable.
It should be noted that above-described embodiment is only the section Example for realizing preferred embodiment of the invention, Er Feiquan
Portion's embodiment.Obviously, it is based on the above embodiment of the present invention, those of ordinary skill in the art are not making creative work
Under the premise of other all embodiments obtained, should fall within the scope of the present invention.
Claims (3)
1. a kind of birch young pilose antler dark green tea with repairing pancreas function, which is characterized in that be prepared by the raw material of following parts by weight
It arrives: 20-40 parts of birch young pilose antler, 60-80 parts of tealeaves, 6-10 parts of cassia seed, 6-8 parts of chrysanthemum, 3-5 parts of the coptis and 5-9 parts of radix bardanae.
2. a kind of birch young pilose antler dark green tea with repairing pancreas function, which is characterized in that be prepared by the raw material of following parts by weight
It arrives: 30 parts of birch young pilose antler, 70 parts of tealeaves, 8 parts of cassia seed, 7 parts of chrysanthemum, 4 parts of the coptis and 7 parts of radix bardanae.
3. a kind of preparation method of the birch young pilose antler dark green tea as claimed in claim 1 or 2 with repairing pancreas function, including following step
It is rapid:
(1) choose by weight without rot, the blocky birch of mildew it is fine and soft, cleaning, which is placed in dryer, to be dried to moisture content and exists
5-8%；
(2) the birch young pilose antler after step (1) drying is placed in pulverizer and is crushed, smashed birch young pilose antler is crossed into 16 meshes, obtains birch
Set fine and soft particle；
(3) by birch young pilose antler particle obtained by step (2) according to solid-liquid ratio 1:40 addition pure water, water-bath 2.5h under the conditions of 60 DEG C, so
0.2 μm of filter filtering supernatant is used afterwards, and supernatant is concentrated into original volume under the conditions of 60 DEG C using Rotary Evaporators
30%, chloroform and n-butanol are configured to Sevage reagent according to volume ratio for 5:1, then by filtrate and Sevage reagent according to body
Product is mixed than 5:1, vibrates 20min deproteinized, centrifuging and taking supernatant, repeating addition Sevage reagent to no white precipitate precipitation is
Only；It is by volume again that 1:4 is mixed by 95% concentration ethanol of supernatant, 4 DEG C of alcohol precipitation 12h, then mixed liquor is set into centrifuge
It is centrifuged 10min under 15000rpm, sediment is finally freeze-dried to obtain birch young pilose antler extract；
(4) by cassia seed, chrysanthemum, the coptis and radix bardanae is selected, cleaning, it is dry be 5-8% to water content after, be placed in pulverizer
Middle crushing smashes it through 16 meshes, obtains cassia seed, chrysanthemum, the coptis, radix bardanae hybrid particles；
(5) by cassia seed, chrysanthemum, the coptis and radix bardanae particle obtained by birch young pilose antler extract, step (4) obtained by step (3) with kill
It is green, just rub after tea mixing be uniformly mixed, then carry out pile-fermentation, room temperature control is 25-30 DEG C, in heap temperature control is
45-55 DEG C, adding water humid control is 80-90%, and time control is 24-36h；
(6) the tealeaves mixture that pile-fermentation is completed is rubbed again, controls the time in 6-8min, is done after the completion of rubbing again
It is pressed into brick tea using mold after dry, baking, obtains finished product after metering packing.
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| CN (1) | CN109430469A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112869156A (en) * | 2021-01-25 | 2021-06-01 | 呼伦贝尔市林海森林经营管理有限公司 | A method for preparing concentrated solution from Inonotus obliquus and birch juice |
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2018
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112869156A (en) * | 2021-01-25 | 2021-06-01 | 呼伦贝尔市林海森林经营管理有限公司 | A method for preparing concentrated solution from Inonotus obliquus and birch juice |
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