CN109415765A - 用纳米孔的聚合物分析中的混合光学信号 - Google Patents

用纳米孔的聚合物分析中的混合光学信号 Download PDF

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Publication number
CN109415765A
CN109415765A CN201780023542.2A CN201780023542A CN109415765A CN 109415765 A CN109415765 A CN 109415765A CN 201780023542 A CN201780023542 A CN 201780023542A CN 109415765 A CN109415765 A CN 109415765A
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CN
China
Prior art keywords
nano
pore
fluorescent marker
monomer
polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780023542.2A
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English (en)
Chinese (zh)
Inventor
布雷特·N·安德森
马丁·胡伯
斯蒂芬·C·梅斯维兹
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantapore Inc
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Quantapore Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Quantapore Inc filed Critical Quantapore Inc
Publication of CN109415765A publication Critical patent/CN109415765A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201780023542.2A 2016-04-14 2017-03-27 用纳米孔的聚合物分析中的混合光学信号 Pending CN109415765A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662322343P 2016-04-14 2016-04-14
US62/322,343 2016-04-14
PCT/US2017/024314 WO2017180319A1 (en) 2016-04-14 2017-03-27 Mixed optical signals in polymer analysis with nanopores

Publications (1)

Publication Number Publication Date
CN109415765A true CN109415765A (zh) 2019-03-01

Family

ID=60042715

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780023542.2A Pending CN109415765A (zh) 2016-04-14 2017-03-27 用纳米孔的聚合物分析中的混合光学信号

Country Status (5)

Country Link
US (2) US20190078158A1 (https=)
EP (1) EP3443122B1 (https=)
JP (1) JP2019513387A (https=)
CN (1) CN109415765A (https=)
WO (1) WO2017180319A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023029044A1 (zh) * 2021-09-06 2023-03-09 百图生科(北京)智能技术有限公司 用于单细胞测序的方法、装置、设备、介质和程序产品

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3031812A1 (en) * 2016-08-19 2018-02-22 Quantapore, Inc. Optically-based nanopore sequencing using quenching agents
GB201902630D0 (en) * 2019-02-27 2019-04-10 Imperial Innovations Ltd Analyte detection method
CA3179981A1 (en) 2020-04-24 2021-10-28 Quantapore, Inc. Fluorescent polynucleotide sequencing methods and compositions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
US20140255935A1 (en) * 2009-09-30 2014-09-11 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
CN105283560A (zh) * 2013-05-24 2016-01-27 昆塔波尔公司 基于纳米孔的通过混合的fret检测的核酸分析

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
WO2012121756A1 (en) * 2011-03-04 2012-09-13 Quantapore, Inc. Apparatus and methods for performing optical nanopore detection or sequencing
WO2014066905A2 (en) * 2012-10-28 2014-05-01 Quantapore, Inc. Reducing background fluorescence in mems materials by low energy ion beam treatment
ES2789000T3 (es) * 2014-10-10 2020-10-23 Quantapore Inc Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente
US20170132361A1 (en) * 2015-10-28 2017-05-11 Pacific Biosciences Of California, Inc. Sequence assembly method
WO2017123647A1 (en) * 2016-01-15 2017-07-20 Quantapore, Inc. Optically-based nanopore analysis with reduced background
JP2019528776A (ja) * 2016-10-10 2019-10-17 クアンタポール， インコーポレイテッド 光学に基づくナノポア配列決定における蛍光標識組成物の制御

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140255935A1 (en) * 2009-09-30 2014-09-11 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
CN105283560A (zh) * 2013-05-24 2016-01-27 昆塔波尔公司 基于纳米孔的通过混合的fret检测的核酸分析

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ANDREW M SMITH等: "Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples", 《NUCLEIC ACIDS RESEARCH》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023029044A1 (zh) * 2021-09-06 2023-03-09 百图生科(北京)智能技术有限公司 用于单细胞测序的方法、装置、设备、介质和程序产品

Also Published As

Publication number Publication date
EP3443122A1 (en) 2019-02-20
US20220170088A1 (en) 2022-06-02
WO2017180319A1 (en) 2017-10-19
US20190078158A1 (en) 2019-03-14
EP3443122B1 (en) 2021-07-07
JP2019513387A (ja) 2019-05-30
EP3443122A4 (en) 2019-12-04

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Application publication date: 20190301