CN109414449A - 利用和不利用可设计核酸酶编辑基因组的改进方法 - Google Patents

利用和不利用可设计核酸酶编辑基因组的改进方法 Download PDF

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CN109414449A
CN109414449A CN201780038175.3A CN201780038175A CN109414449A CN 109414449 A CN109414449 A CN 109414449A CN 201780038175 A CN201780038175 A CN 201780038175A CN 109414449 A CN109414449 A CN 109414449A
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oligonucleotides
editor
target
dna
modification
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托德·M·伍尔夫
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7115—Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/712—Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201780038175.3A 2016-05-06 2017-05-05 利用和不利用可设计核酸酶编辑基因组的改进方法 Pending CN109414449A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662333004P 2016-05-06 2016-05-06
US62/333,004 2016-05-06
US201662410487P 2016-10-20 2016-10-20
US62/410,487 2016-10-20
PCT/US2017/031381 WO2017193053A1 (en) 2016-05-06 2017-05-05 Improved methods for genome editing with and without programmable nucleases

Publications (1)

Publication Number Publication Date
CN109414449A true CN109414449A (zh) 2019-03-01

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CN201780038175.3A Pending CN109414449A (zh) 2016-05-06 2017-05-05 利用和不利用可设计核酸酶编辑基因组的改进方法

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Country Link
US (1) US20190300872A1 (https=)
EP (2) EP4023228A1 (https=)
JP (4) JP6872560B2 (https=)
CN (1) CN109414449A (https=)
CA (1) CA3022319A1 (https=)
WO (1) WO2017193053A1 (https=)

Cited By (2)

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CN111378051A (zh) * 2020-03-25 2020-07-07 北京市农林科学院 Pe-p2引导编辑系统及其在基因组碱基编辑中的应用
CN113637670A (zh) * 2021-04-27 2021-11-12 中国药科大学 一种用于任意基因表达调控的组合物及方法

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JP6872560B2 (ja) * 2016-05-06 2021-05-19 エム． ウルフ、トッド プログラム可能ヌクレアーゼのあるゲノム編集及びプログラム可能ヌクレアーゼのないゲノム編集のための改善された方法
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US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
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CN111378051A (zh) * 2020-03-25 2020-07-07 北京市农林科学院 Pe-p2引导编辑系统及其在基因组碱基编辑中的应用
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CN113637670A (zh) * 2021-04-27 2021-11-12 中国药科大学 一种用于任意基因表达调控的组合物及方法

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JP6872560B2 (ja) 2021-05-19
EP3452055A4 (en) 2019-11-06
JP2024088642A (ja) 2024-07-02
EP3452055A1 (en) 2019-03-13
US20190300872A1 (en) 2019-10-03
CA3022319A1 (en) 2017-11-09
JP2019514405A (ja) 2019-06-06
JP2023011676A (ja) 2023-01-24
EP4023228A1 (en) 2022-07-06
JP2021119776A (ja) 2021-08-19
WO2017193053A1 (en) 2017-11-09

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Application publication date: 20190301