CN109401956B - Temperature detector for PCR instrument - Google Patents
Temperature detector for PCR instrument Download PDFInfo
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- CN109401956B CN109401956B CN201811520121.3A CN201811520121A CN109401956B CN 109401956 B CN109401956 B CN 109401956B CN 201811520121 A CN201811520121 A CN 201811520121A CN 109401956 B CN109401956 B CN 109401956B
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- temperature
- circuit board
- test tube
- temperature sensor
- pcr instrument
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
Abstract
The invention discloses a temperature detector for temperature performance of each hole of a PCR (polymerase chain reaction) instrument, which comprises a temperature sensor, a test tube compatible with a PCR structural hole tube, a cover plate, a heat-conducting silicon film, a flexible circuit board and a multi-channel high-precision multimeter. The temperature detector provided by the invention has the advantages of simple structure, sensitive detection, stable and reliable performance, convenience in use and suitability for detecting the temperature performance of various PCR instruments.
Description
Technical Field
The invention relates to the field of temperature performance detection of a PCR instrument, in particular to a structural system for detecting the temperature performance of each hole site of the PCR instrument.
Background
Briefly, PCR is the mass synthesis of In Vitro or In Vitro of a specific gene using DNA polymerase, which basically involves the specific linkage replication using DNA polymerase. The PCR instrument is an instrument for providing the whole PCR process. In order to provide a good PCR environment, a temperature control unit is necessarily included in the PCR instrument. This unit also necessarily needs to have controlled temperature performance: temperature accuracy, temperature uniformity, rapid temperature rise and fall, and the like. The commonly used PCR instruments have a throughput of 8, 12, 24, 48, 96, 384, etc. as required for various applications. The temperature of these multi-throughput PCR instruments needs to be well controlled to ensure the consistency and repeatability of the PCR process.
In order to control the temperature of the flux corresponding positions well, the positions need to be detected accurately in real time, and an effective tool for detecting the temperature system of the PCR instrument is needed for evaluating the temperature performance of the PCR instrument or for after-sales maintenance of the temperature system of the PCR instrument. The invention relates to a temperature detector for detecting the temperature performance of a PCR instrument. The commonly used detection of the temperature point of the PCR instrument is usually a temperature sensor of a single-path or multi-path thermometer, and the temperature sensor is inserted into the hole site of the PCR instrument for detection. The temperature detector can conveniently and practically solve the problems and ensure the requirements of temperature detection precision, stability and real-time temperature distribution.
Disclosure of Invention
The invention aims to provide a temperature detector for conveniently and accurately detecting the temperature performance of a PCR instrument, which has the advantages of simple structure, convenient use, good reliability, good stability and good real-time property, and can be compatible with various common PCR instruments as far as possible.
The invention provides a system solution for detecting the temperature performance of a PCR instrument, which comprises the following steps:
a temperature detector for a PCR instrument comprises a test tube with a temperature sensor, a fluid medium, a circuit board, a cover plate and a temperature acquisition meter; the test tube with the temperature sensor is arranged on the circuit board, the temperature sensor and the fluid medium are arranged in the test tube, the cover plate covers the test tube, and the circuit board is connected to the temperature acquisition meter through the connector.
The temperature sensor includes a thermistor and a thermocouple.
The fluid medium submerges the temperature sensor; the fluid medium is water, silicon oil, heat-conducting glue or latex.
The fluid medium is latex with high heat conductivity coefficient.
The circuit board is provided with an interface compatible with the temperature sensor; the circuit board is also provided with a circuit board wire or a small hole for fixing the sensor connecting wire.
The cover plate is made of metal materials.
The detector further comprises a heat-conducting silica gel film, and the heat-conducting silica gel film is attached to the cover plate.
The circuit board comprises a circuit board made of bakelite, a flexible circuit board and an aluminum-based circuit board.
The circuit board is a flexible circuit board, and the flexible circuit board is single-layer wiring or multi-layer wiring.
And the circuit board finishes data acquisition through an acquisition meter, and the acquisition meter comprises a multi-channel temperature acquisition card, a multi-channel resistance acquisition meter and a multi-channel temperature acquisition meter.
The invention also claims the application of the temperature detector, and the temperature detector is used for detecting the temperature performance of each hole site of the PCR instrument.
The PCR instrument is a multi-flux PCR instrument.
The multi-throughput PCR instrument includes, but is not limited to, 8, 12, 24, 48, 96, 384 throughput.
The invention claims a test tube, which is used for a temperature detector for a PCR instrument and is provided with a temperature sensor, and the temperature sensor is submerged by a fluid medium.
The test tube is compatible with the PCR structure hole tube; the fluid medium is water, silicon oil, heat-conducting glue or latex.
The fluid medium is latex with high thermal conductivity.
The latex is a settable latex.
The invention also claims the application of the test tube, which is used for a temperature detector aiming at the PCR instrument.
The test tube is mounted on the circuit board.
And pins of the temperature sensors in the test tubes are welded to corresponding welding pads of the circuit board.
The more specific technical scheme of the invention is as follows: a temperature detector for detecting the temperature performance of a PCR instrument. The temperature detector comprises a test tube with a temperature sensor, latex with high heat conductivity coefficient, a circuit board, a cover plate, a heat-conducting silicone membrane and a multi-channel high-precision multimeter. The test tube with the temperature sensor, install in on the circuit board, the test tube in install the high accuracy temperature sensor with high coefficient of thermal conductivity's latex, apron lid on the test tube, the attached on the apron of heat conduction silica gel membrane, the circuit board pass through the connector and be connected to multichannel high accuracy universal meter.
Further, the test tube with the temperature sensor is provided with a temperature sensor, and the temperature sensor is not limited to a thermistor or a thermocouple and is used for testing the temperature in the test tube. The test tube with temperature sensor is not limited to a specific test tube, and it is within the scope of the present invention to be compatible with a PCR instrument of a specific type.
Furthermore, the test tube with the temperature sensor is not only provided with the temperature sensor, but also is flooded by fluid medium, the medium is not limited to water, silicon oil and heat-conducting glue, the functions of the media are mainly that the temperature of the medium in the tube can be well detected by the sensor, the temperature is not influenced by the temperature difference between the tube wall and the center of the tube, and the temperature condition of the reagent applied by the actual PCR instrument can be simulated.
Furthermore, the medium in the test tube with the temperature sensor, preferably latex with high thermal conductivity, can be solidified. The effect of this heat conduction latex is that the temperature of medium in the pipe has been represented better, and the temperature is not stratified, moreover because the solidification of this heat conduction latex can good fixed sensor for the difficult emergence of state change of sensor, thereby the repeatability of temperature detection can obtain the biggest improvement. The heat-conducting latex is not easy to volatilize and run off, so that the medium is not required to be repeatedly added into the detector, and the stability and the reliability of temperature detection can be correspondingly improved.
Further, the test tube with the temperature sensor is placed on the circuit board. The circuit board has an interface compatible with the temperature sensor, and the interface is not limited to a connector and a bonding pad. The circuit board can also be provided with circuit board wiring or a small hole for fixing the sensor connecting wire.
Furthermore, the circuit board is not limited to a common bakelite circuit board, a flexible circuit board and an aluminum-based circuit board. In order to adapt to various models of PCR instruments, the circuit board is preferably a flexible circuit board. The flexible circuit board is not limited to a single-layer wiring or a multi-layer wiring. The flexible circuit board has the advantages that the structure is simpler due to the wiring, and due to the characteristics of bending and deformation, the detector is compatible with various PCR instruments as much as possible, the application state of the PCR instruments is changed as little as possible, and the temperature performance of the PCR instruments is reflected more truly.
Furthermore, the cover plate is made of metal material, and the main function is to uniformly conduct the heat on the internal heat cover plate of the PCR instrument to the upper surface of the test tube with the temperature sensor for good heat conduction, so that the temperature of the test tube is as close as possible to the temperature of the test tube in the actual PCR process. The cover plate has the second function of blocking the test tube with the temperature sensor so that the test tube cannot move freely, and therefore the stability of the test tube is improved.
Furthermore, the heat-conducting silicone rubber film is attached to the cover plate and located between the cover plate and the test tube with the temperature sensor, and the heat-conducting silicone rubber film is mainly used for insulating pins of the sensor of the test tube with the temperature sensor.
Furthermore, the circuit board finishes data acquisition through an acquisition table. The acquisition meter is not limited to a multi-channel temperature acquisition card, a multi-channel resistance acquisition meter and a multi-channel temperature acquisition meter. In order to improve the sensitivity and the accuracy of system detection, the optimal configuration of the acquisition meter is a multi-channel high-precision multimeter with high-speed scanning. The high-speed scanning multi-channel high-precision multimeter has the advantages that the high-precision acquisition performance is realized, the multi-channel scanning is realized, and the temperature process can be well displayed.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required in the embodiments will be briefly described below. It is appreciated that the following drawings depict only certain embodiments of the invention and are therefore not to be considered limiting of its scope. For a person skilled in the art, it is possible to derive other relevant figures from these figures without inventive effort.
FIG. 1 is an exploded view of a temperature detector according to an embodiment of the present invention;
FIG. 2 is a schematic illustration of a test tube provided in accordance with an embodiment of the present invention;
description of the reference numerals: a cover plate 10; a heat-conducting silicone adhesive film 20; test tubes 30, 304 with temperature sensors; a circuit board 40; a multi-channel high-precision multimeter 50; a fluid medium, latex 302; pins 301, 303 of temperature sensor 305.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "inside", "outside", "left", "right", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or the orientations or positional relationships that the products of the present invention are conventionally placed in use, or the orientations or positional relationships that are conventionally understood by those skilled in the art, and are used for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is also to be noted that, unless otherwise explicitly stated or limited, the terms "disposed" and "connected" are to be interpreted broadly, and for example, "connected" may be a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; the connection may be direct or indirect through an intermediate medium, and the connection may be internal to the two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The following detailed description of embodiments of the invention refers to the accompanying drawings.
Examples
Referring to fig. 1 to fig. 2, the present embodiment provides a temperature detector, which has a simple structure, is convenient to use, is stable and reliable, has good real-time performance, and can read the temperature distribution of the PCR instrument rapidly and precisely.
It should be noted that the temperature detector provided in this embodiment is mainly applied to the temperature detection of a 96-well PCR instrument. The temperature detector is placed into the PCR instrument according to the position of a standard 96-pore plate, the multichannel high-precision thermometer 50 is started to scan the temperature of the temperature detector, the PCR instrument operates according to a normal application flow, the temperature detector can automatically scan the temperature of the 96-pore plate in the PCR instrument in real time, and high-precision temperature data are stored.
The temperature detector provided by the embodiment comprises a test tube 30 with a temperature sensor, latex 302 with high heat conductivity coefficient, a circuit board 40, a cover plate 10, a heat-conducting silicone film 20 and a multi-channel high-precision multimeter 50. The test tube 30 with the temperature sensor is arranged on the circuit board 40, the high-precision temperature sensor 305 and the latex 302 with the high heat conductivity coefficient are arranged in the test tube 30, the cover plate 10 covers the test tube 30, and the flexible circuit board 40 is connected to the multi-channel high-precision multimeter 50 through an interface terminal.
The test tube 30 with a temperature sensor provided in this embodiment is installed with a temperature sensor 305, and the temperature sensor 305 is not limited to a thermistor or a thermocouple, and is used for measuring the temperature in the test tube. Pins 301, 303 of the temperature sensor 305 are soldered to corresponding pads on the flexible circuit board 40. The thermistor with the optimal selection of PT 1000/3B high-precision grade is provided by the embodiment, and the thermistor not only has sensitive temperature response, but also has high repeatability precision and good linearity. The test tube 304 with a temperature sensor provided in this embodiment is not limited to a specific test tube, and is within the scope of the present invention as long as it is compatible with a PCR instrument of a certain type.
The test tube 30 with the temperature sensor provided by the embodiment is not only provided with the temperature sensor 305, but also is filled with the fluid medium 302, the medium is not limited to water, silicon oil and heat conducting glue, and the functions of the media are mainly that the temperature of the medium in the tube can be well detected by the sensor, the temperature is not influenced by the temperature difference between the tube wall and the tube center, and the temperature condition of the reagent applied by the actual PCR instrument can be simulated.
The medium 302 of the test tube 30 with the temperature sensor provided in this embodiment is preferably latex 302 with high thermal conductivity, and can be solidified in 24 hours. The latex 302 with high thermal conductivity coefficient is subjected to operations such as quantification, centrifugation and the like, so that the consistency of the installation conditions of various sensors can be better ensured. This long-term coagulation of the latex 302 with high thermal conductivity also facilitates the installation of the sensor 305. The temperature mixing effect of the latex 302 with the high heat conductivity coefficient is the temperature of a medium in a better uniform mixing pipe, the temperature is not layered, and the sensor 305 can be well fixed due to the solidification effect of the heat conducting rubber 302, so that the state of the sensor 305 is not easy to change, and the repeatability of temperature detection can be improved to the maximum. The latex 302 with high thermal conductivity is not easy to volatilize and run off, so that the detector does not need to add the medium repeatedly.
The test tube 30 with the temperature sensor provided in this embodiment is placed on the circuit board 40. The present embodiment provides the circuit board 40 with an interface compatible with the temperature sensor 305, and the interface is not limited to a connector and a pad. The interface provided by the embodiment is a bonding pad, and can stably and reliably fix the temperature sensor 305, so that the structure is neat and uniform.
The circuit board 40 provided in this embodiment is not limited to a common bakelite circuit board, a flexible circuit board, and an aluminum-based circuit board. In order to adapt to various models of PCR instruments, the circuit board provided by the embodiment is the flexible circuit board. The flexible circuit board 40 is used for not only routing wires to enable the structure to be simpler and more reliable and the resistance on the wires to be controllable, but also enabling the detector provided by the embodiment to be compatible with various PCR instruments as much as possible due to the characteristics of bending and deformation of the flexible circuit board, changing the application state of the PCR instruments as little as possible and reflecting the temperature performance of the PCR instruments more truly.
The cover plate 10 provided in this embodiment is made of a metal material, and the main function is to uniformly conduct the heat on the internal heat cover plate of the PCR instrument to the upper surface of the test tube 30 with the temperature sensor provided in this embodiment, so that the temperature of the test tube 30 is as close as possible to the temperature of the test tube in the actual PCR process. The second function of the cover plate 10 is to block the test tube 30 with the temperature sensor provided in the embodiment from moving freely, thereby improving the stability of the test tube 30.
The heat conductive silicone adhesive film 20 provided in this embodiment mainly serves to insulate and place the pins 301 and 303 of the sensor 305 of the test tube 30 with a temperature sensor provided in this embodiment, and this scheme is a preferable scheme, and is not limited to whether the heat conductive silicone adhesive film 20 is actually added.
The circuit board 40 provided in this embodiment finally completes data acquisition through the acquisition table 50. The collection meter 50 provided in this embodiment is not limited to a multi-channel temperature collection card, a multi-channel resistance collection meter, or a multi-channel temperature collection meter. In order to improve the sensitivity and accuracy of system detection, the optimal configuration of the acquisition meter 50 provided by the present embodiment is a multi-channel high-accuracy multimeter 50 with high-speed scanning. The multi-channel high-precision multimeter 50 for high-speed scanning provided by the embodiment has the functions of high-precision acquisition performance and multi-channel scanning, can well display a temperature process, and simultaneously stores responsive temperature data.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A temperature detector for accurately detecting the temperature performance of a PCR instrument is characterized by comprising a test tube with a temperature sensor, a fluid medium, a circuit board, a cover plate and a temperature acquisition meter; the test tube with the temperature sensor is arranged on the circuit board, the temperature sensor and the fluid medium are arranged in the test tube, the cover plate is made of metal materials and covers the test tube, heat on the internal heat cover plate of the PCR instrument is uniformly conducted onto the test tube with the temperature sensor, and the circuit board is connected to a temperature acquisition meter through a connector; two pins of the temperature sensor are welded on corresponding bonding pads of the flexible circuit board;
the fluid medium submerges the temperature sensor; the fluid medium is water, silicon oil, heat-conducting glue or latex.
2. The temperature detector for accurately detecting the temperature performance of the PCR instrument as claimed in claim 1, wherein the circuit board comprises a bakelite circuit board or a flexible circuit board.
3. The temperature detector for accurately detecting the temperature performance of the PCR instrument as claimed in claim 2, wherein the circuit board is a flexible circuit board, and the flexible circuit board is a single-layer wiring or a multi-layer wiring.
4. The temperature detector according to claim 3, wherein the circuit board completes data collection via a collection table, the collection table comprises a multi-channel temperature collection card, a multi-channel resistance collection table, and a multi-channel temperature collection table.
5. The use of the temperature detector for accurately detecting the temperature performance of a PCR instrument as set forth in any one of claims 1 to 4, wherein the temperature performance of each well site of the PCR instrument is detected.
6. Test tube for a temperature detector for a PCR instrument according to one of claims 1 to 5, characterized in that a temperature sensor is installed, which is flooded with a fluid medium.
7. The test tube of claim 6, wherein the test tube is compatible with a PCR structured well tube; the fluid medium is water, silicon oil, heat-conducting glue or latex.
8. The test tube of claim 7, wherein the fluid medium is a high thermal conductivity latex.
9. Use of a test tube according to claim 7 or 8 for a temperature detector for a PCR apparatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811520121.3A CN109401956B (en) | 2018-12-12 | 2018-12-12 | Temperature detector for PCR instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811520121.3A CN109401956B (en) | 2018-12-12 | 2018-12-12 | Temperature detector for PCR instrument |
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| CN109401956A CN109401956A (en) | 2019-03-01 |
| CN109401956B true CN109401956B (en) | 2023-01-03 |
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| CN201811520121.3A Active CN109401956B (en) | 2018-12-12 | 2018-12-12 | Temperature detector for PCR instrument |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111057642A (en) * | 2019-12-12 | 2020-04-24 | 南京信息职业技术学院 | Temperature calibration device for PCR instrument |
| CN112945420A (en) * | 2021-02-02 | 2021-06-11 | 四川淯源仪器科技有限公司 | Temperature calibration system of wireless PCR instrument |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061015A (en) * | 2009-11-18 | 2011-05-18 | 东台百地医用制品有限公司 | Heat conducting susceptibility latex product and preparation method thereof |
| CN204556112U (en) * | 2015-03-25 | 2015-08-12 | 深圳市中图仪器科技有限公司 | A kind of PCR instrument elasticity connects high sensitivity temperature acquisition measuring head structure |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6477479B1 (en) * | 1998-12-11 | 2002-11-05 | Symyx Technologies | Sensor array for rapid materials characterization |
| FR2941960B1 (en) * | 2009-02-11 | 2013-11-01 | Inst Nat De La Rech Agronomique Inra | METHOD FOR TESTING THE THERMAL REGULATION OF A THERMOCYCLE FOR PCR, AND MEANS FOR ITS IMPLEMENTATION |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061015A (en) * | 2009-11-18 | 2011-05-18 | 东台百地医用制品有限公司 | Heat conducting susceptibility latex product and preparation method thereof |
| CN204556112U (en) * | 2015-03-25 | 2015-08-12 | 深圳市中图仪器科技有限公司 | A kind of PCR instrument elasticity connects high sensitivity temperature acquisition measuring head structure |
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