CN109369434A - A kind of ornithine washes brilliant treatment process - Google Patents
A kind of ornithine washes brilliant treatment process Download PDFInfo
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- CN109369434A CN109369434A CN201811553829.9A CN201811553829A CN109369434A CN 109369434 A CN109369434 A CN 109369434A CN 201811553829 A CN201811553829 A CN 201811553829A CN 109369434 A CN109369434 A CN 109369434A
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- ornithine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
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- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
A kind of ornithine washes brilliant treatment process, belongs to product purification technical field.It is washed in brilliant tank it is characterized by: crude product or substandard product are placed in, then 0.8 ~ 1.2 times of ethanol water of crude product or substandard product quality is added, the mass concentration of the ethanol water is 75% ~ 90%, crystalline substance is washed in stirring under 5 DEG C ~ 15 DEG C of wash temperature, 20 min of mixing time ~ 80min, then it is separated by solid-liquid separation, the solid of separation of solid and liquid is dried to obtain the final product.The present invention carries out substandard product as cleaning solution to wash crystalline substance using certain density ethyl alcohol, crystal surrounding impurities are washed away, selected cleaning solution can quickly remove impurity will not dissolve excessive ornithine again, product and cleaning solution are easy to separate and recover simultaneously, reach and improve product quality purpose, while reaching raising yield to reduce cost purpose.
Description
Technical field
A kind of ornithine washes brilliant treatment process, belongs to product purification technical field.
Background technique
Ornithine, molecular formula H2NCH2CH2CH2CH(NH2) COOH, it is a kind of basic amino acid.Though in usual protein
The antibiotic property that (20 kinds of amino acid for being not belonging to constitutive protein matter) cannot be found, but be present in tyrocidine, gramicidin S etc.
In peptide, δ-N- acetylornithice is in addition had found from the corydalis root of remote mountains.It is to be decomposed by arginine for alkali or arginase effect
It generates.In vivo, ornithine is primarily involved in uric acid circulation, plays an important role for the discharge of internal ammoniacal nitrogen.Ornithine
In medicine in addition to as reagent and injection, usually it is used to configure the fizz to recover from fatigue together with arginine.In recent years
Come, ornithine in food industry and medical industry using increasingly extensive.
The prior art mode taken unqualified to product quality is generally means re-crystallization, the mode of recrystallization, yield
Generally 80% or so, yield is relatively low, will increase production cost using process above.
Summary of the invention
The technical problem to be solved by the present invention is overcoming the deficiencies of the prior art and provide, one kind is simple and fast, the rate of recovery is high
Ornithine wash brilliant treatment process.
The technical solution adopted by the present invention to solve the technical problems is: the ornithine washes brilliant treatment process, feature
It is: crude product or substandard product is placed in and washed in brilliant tank, is then added the 0.8 ~ 1.2 of crude product or substandard product quality
Ethanol water again, the mass concentration of the ethanol water are 75% ~ 90%, stir and wash under 5 DEG C ~ 15 DEG C of wash temperature
Crystalline substance, 20 min of mixing time ~ 80min, is then separated by solid-liquid separation, and the solid of separation of solid and liquid is dried.
The present invention provides a kind of easy to operate, and rational technology, convenient, can improve product quality and yield as far as possible reduces
Production cost.The present invention carries out substandard product as cleaning solution to wash crystalline substance using certain density ethyl alcohol, by crystal week
It encloses impurity to wash away, selected cleaning solution can quickly remove impurity will not dissolve excessive ornithine again, while product and wash
It washs liquid to be easy to separate and recover, reaches and improve product quality purpose, while reaching raising yield to reduce cost purpose.
Preferably, the additional amount of the ethanol water is 0.95 ~ 1.05 times of crude product or substandard product quality.
It is more thorough that preferable amount removes impurity.
Preferably, the mass concentration of the ethanol water is 79% ~ 85%.Impurity separates more under preferred mass concentration
Fastly.
Preferably, the wash temperature is 8 DEG C ~ 10 DEG C.Impurity separation faster, removes impurity under preferred wash temperature
More thoroughly.
Preferably, the mixing time is 20 min ~ 25min.It can be shortened under optimum condition and wash the brilliant time.
Preferred wash conditions can improve the quality and yield of product simultaneously after combining.
Preferably, the separation of solid and liquid is by the way of centrifuge separation.
Preferably, the solid of the separation of solid and liquid repeat this stirring wash brilliant technique it is at least secondary after be dried.It carries out
Product quality is more preferable after repeated washing.
Preferably, described to be separated by solid-liquid separation separated liquid and carry out after distillation refines out ethyl alcohol, then adjust mass concentration to
It is reused after 75% ~ 90%.
Compared with prior art, possessed beneficial effect of the invention is: using a kind of certain density ethyl alcohol conduct
Cleaning solution is washed to substandard product and (washes crystalline substance), avoid dissolution substandard product recrystallize, and caused by lose, pass through
(washing crystalline substance) crystal surrounding impurities are washed, product quality is improved.Make to operate simpler quick, the rate of recovery reaches 95% or more.
Specific embodiment
The present invention will be further described combined with specific embodiments below, and wherein embodiment 1 is best implements.
Embodiment 1
Substandard product is placed in and is washed in brilliant tank, 1 times of ethanol water of crude product or substandard product quality is then added,
The mass concentration of ethanol water is 82%, and crystalline substance is washed in stirring under 9 DEG C of wash temperature, mixing time 20min, then using from
The mode of heart separation is separated by solid-liquid separation, be separated by solid-liquid separation separation liquid carry out distillation it is exquisite go out ethyl alcohol after, then after adjusting mass concentration
Reuse, by the solid of separation of solid and liquid repeat this stirring wash brilliant technique it is at least secondary after be drying to obtain.
Embodiment 2
Substandard product is placed in and is washed in brilliant tank, crude product is then added or 1.05 times of ethyl alcohol of substandard product quality is water-soluble
Liquid, the mass concentration of ethanol water are 85%, and crystalline substance is washed in stirring under 8 DEG C of wash temperature, then mixing time 22min is used
The mode of centrifuge separation is separated by solid-liquid separation, and the liquid for being separated by solid-liquid separation separation carries out after distilling exquisite ethyl alcohol out, then adjusts mass concentration
After reuse, by the solid of separation of solid and liquid repeat this stirring wash brilliant technique it is at least secondary after be drying to obtain.
Embodiment 3
Substandard product is placed in and is washed in brilliant tank, crude product is then added or 0.95 times of ethyl alcohol of substandard product quality is water-soluble
Liquid, the mass concentration of ethanol water are 79%, and crystalline substance is washed in stirring under 10 DEG C of wash temperature, then mixing time 25min is adopted
Be separated by solid-liquid separation with the mode of centrifuge separation, be separated by solid-liquid separation separation liquid carry out distillation it is exquisite go out ethyl alcohol after, then to adjust quality dense
Reused after degree, by the solid of separation of solid and liquid repeat this stirring wash brilliant technique it is at least secondary after be drying to obtain.
Embodiment 4
Substandard product is placed in and is washed in brilliant tank, crude product is then added or 0.8 times of ethyl alcohol of substandard product quality is water-soluble
Liquid, the mass concentration of ethanol water are 75%, and crystalline substance is washed in stirring under 15 DEG C of wash temperature, then 65 min of mixing time is adopted
Be separated by solid-liquid separation with the mode of centrifuge separation, be separated by solid-liquid separation separation liquid carry out distillation it is exquisite go out ethyl alcohol after, then to adjust quality dense
Reused after degree, by the solid of separation of solid and liquid repeat this stirring wash brilliant technique it is at least secondary after be drying to obtain.
Embodiment 5
Substandard product is placed in and is washed in brilliant tank, crude product is then added or 1.2 times of ethyl alcohol of substandard product quality is water-soluble
Liquid, the mass concentration of ethanol water are 90%, and crystalline substance is washed in stirring under 5 DEG C of wash temperature, then mixing time 80min is used
The mode of centrifuge separation is separated by solid-liquid separation, and the liquid for being separated by solid-liquid separation separation carries out after distilling exquisite ethyl alcohol out, then adjusts mass concentration
After reuse, by the solid of separation of solid and liquid repeat this stirring wash brilliant technique it is at least secondary after be drying to obtain.
Comparative example 1
Basic technology is with embodiment 1, the difference is that the mass concentration of ethanol water used is 50%.
Comparative example 2
Basic technology is with embodiment 1, the difference is that replacing ethanol water with dehydrated alcohol.
Comparative example 3
Basic technology is with embodiment 1, the difference is that 25 DEG C of wash temperature position.
Embodiment and comparative example products obtained therefrom detection method: detecting instrument: 721 visible spectrophotometers
1 the performance test results of table
。
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But without departing from the technical solutions of the present invention, according to the technical essence of the invention to above embodiments institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection scope of technical solution of the present invention.
Claims (8)
1. a kind of ornithine washes brilliant treatment process, it is characterised in that: be placed in crude product or substandard product and wash in brilliant tank, so
0.8 ~ 1.2 times of ethanol water of crude product or substandard product quality, the mass concentration of the ethanol water are added afterwards
It is 75% ~ 90%, crystalline substance is washed in stirring under 5 DEG C ~ 15 DEG C of wash temperature, then 20 min of mixing time ~ 80min are separated by solid-liquid separation, will
The solid of separation of solid and liquid is dried.
2. a kind of ornithine according to claim 1 washes brilliant treatment process, it is characterised in that: the ethanol water
Additional amount be 0.95 ~ 1.05 times of crude product or substandard product quality.
3. a kind of ornithine according to claim 1 washes brilliant treatment process, it is characterised in that: the ethanol water
Mass concentration be 79% ~ 85%.
4. a kind of ornithine according to claim 1 washes brilliant treatment process, it is characterised in that: the wash temperature is
8℃~10℃。
5. a kind of ornithine according to claim 1 washes brilliant treatment process, it is characterised in that: the mixing time is
20 min~25 min。
6. a kind of ornithine according to claim 1 washes brilliant treatment process, it is characterised in that: the separation of solid and liquid is adopted
With the mode of centrifuge separation.
7. a kind of ornithine according to claim 1 washes brilliant treatment process, it is characterised in that: the separation of solid and liquid
Solid repeat this stirring wash brilliant technique it is at least secondary after be dried.
8. a kind of ornithine according to claim 1 or claim 7 washes brilliant treatment process, it is characterised in that: the separation of solid and liquid
Separated liquid carries out after distillation refines out ethyl alcohol, then adjusts mass concentration to reusing after 75% ~ 90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811553829.9A CN109369434B (en) | 2018-12-18 | 2018-12-18 | Crystal washing treatment process of ornithine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811553829.9A CN109369434B (en) | 2018-12-18 | 2018-12-18 | Crystal washing treatment process of ornithine |
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| Publication Number | Publication Date |
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| CN109369434A true CN109369434A (en) | 2019-02-22 |
| CN109369434B CN109369434B (en) | 2021-08-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811553829.9A Active CN109369434B (en) | 2018-12-18 | 2018-12-18 | Crystal washing treatment process of ornithine |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090082594A1 (en) * | 2004-11-15 | 2009-03-26 | Kyowa Hakko Kogyo Co., Ltd. | Crystals of l-ornithine and process for producing the same |
| CN102041282A (en) * | 2009-10-14 | 2011-05-04 | 新沂市汉菱生物工程有限公司 | Method for preparing L-ornithine by utilizing enzyme method |
| CN102191291A (en) * | 2011-04-25 | 2011-09-21 | 武汉远大弘元股份有限公司 | Method for producing L-ornithine hydrochloride by genetic engineering bacteria |
| CN107325016A (en) * | 2017-08-20 | 2017-11-07 | 合肥信达膜科技有限公司 | A kind of high-quality ornithine extracting method |
| CN107916282A (en) * | 2017-12-13 | 2018-04-17 | 湖北新生源生物工程有限公司 | A kind of method that bioanalysis prepares L citrulling and L ornithines |
-
2018
- 2018-12-18 CN CN201811553829.9A patent/CN109369434B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090082594A1 (en) * | 2004-11-15 | 2009-03-26 | Kyowa Hakko Kogyo Co., Ltd. | Crystals of l-ornithine and process for producing the same |
| CN102041282A (en) * | 2009-10-14 | 2011-05-04 | 新沂市汉菱生物工程有限公司 | Method for preparing L-ornithine by utilizing enzyme method |
| CN102191291A (en) * | 2011-04-25 | 2011-09-21 | 武汉远大弘元股份有限公司 | Method for producing L-ornithine hydrochloride by genetic engineering bacteria |
| CN107325016A (en) * | 2017-08-20 | 2017-11-07 | 合肥信达膜科技有限公司 | A kind of high-quality ornithine extracting method |
| CN107916282A (en) * | 2017-12-13 | 2018-04-17 | 湖北新生源生物工程有限公司 | A kind of method that bioanalysis prepares L citrulling and L ornithines |
Non-Patent Citations (2)
| Title |
|---|
| 万红贵 等: "L-鸟氨酸的制备及应用", 《生物加工过程》 * |
| 郝永兵 等: "L-鸟氨酸的化学合成方法", 《氨基酸和生物资源》 * |
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| CN109369434B (en) | 2021-08-24 |
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Denomination of invention: A crystal washing process for ornithine Effective date of registration: 20220601 Granted publication date: 20210824 Pledgee: Zibo Branch of Bank of Communications Co.,Ltd. Pledgor: SHANDONG JINYANG PHARMACEUTICAL Co.,Ltd. Registration number: Y2022980006929 |
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Date of cancellation: 20230529 Granted publication date: 20210824 Pledgee: Zibo Branch of Bank of Communications Co.,Ltd. Pledgor: SHANDONG JINYANG PHARMACEUTICAL Co.,Ltd. Registration number: Y2022980006929 |
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Denomination of invention: A crystal washing treatment process for ornithine Effective date of registration: 20230530 Granted publication date: 20210824 Pledgee: Zibo Branch of Bank of Communications Co.,Ltd. Pledgor: SHANDONG JINYANG PHARMACEUTICAL Co.,Ltd. Registration number: Y2023980042228 |
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