CN106748847A - A kind of extracting method of L alanine - Google Patents
A kind of extracting method of L alanine Download PDFInfo
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- CN106748847A CN106748847A CN201611020059.2A CN201611020059A CN106748847A CN 106748847 A CN106748847 A CN 106748847A CN 201611020059 A CN201611020059 A CN 201611020059A CN 106748847 A CN106748847 A CN 106748847A
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- alanine
- zymotic fluid
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- flocculation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
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- Treatment Of Liquids With Adsorbents In General (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention discloses a kind of extracting method of L alanine, the method is included in order to adding the first flocculant, the second flocculant and the 3rd flocculant to be flocculated in the L alanine zymotic fluid, separated in the L alanine zymotic fluids after stirring, precipitation process are put into vacuum drum filter plant, obtained flocculation clarified solution；The flocculation clarified solution is crystallized；Will obtain crystalline solid drying both the L alanine.The method that the application is provided obtains the impurity such as thalline, protein, phosphate, residual sugar in removing zymotic fluid using flocculence precipitation, eliminate calcium precipitation in the prior art and cross the operations such as film removal of impurities, extraction process is enormously simplify, equipment investment is reduced, and improve product quality.Using crystalline mother solution and wash brilliant residual water come back to flocculation, decolouring, concentrate drying circulation in.Loss of material is avoided, environmental protection pressure is reduced, yield, energy-conserving and environment-protective is improved.
Description
Technical field
The present invention relates to fermentation technical field, more particularly to a kind of extracting method of ALANINE.
Background technology
ALANINE (L-Alanine), also known as L- α-alanines, are a kind of important natural amino acids, are contained in blood
The most amino acid of amount, it is closely related with glycometabolism, it is main amino the Worker's Stadium in transamination reaction, with important physiology work(
Energy.It is a kind of nutritional supplement, is the chief component of various Hausmam Amin 20s, can also be made in the middle of medicine
Body, is the primary raw material of vitamin B6.Food additives are alternatively arranged as, nutritive value of food are improved, is improved artificial synthesized sweet
The sense of taste of taste agent.
With continuous research and development and exploitation to ALANINE, its application in fields such as food and medicines constantly expands, and
There is growing trend.But later must be developed to ALANINE, the beginning of the eighties just produces in Japanese industry metaplasia.China was in 80 years
There is small lot batch manufacture for end, industrialized production is just at the early-stage.The production method of ALANINE has proteolysis extraction method, chemistry
Synthetic method, fermentation method are extracted and enzyme transforming process production.Because fermentation method produces ALANINE low cost, and meet green health
Consumption concept, with wide market prospects.After fermentation method production ALANINE terminates, typically carried using more subcrystalline method
Pure ALANINE, typically contains part ALANINE in crystal waste, organic matter in addition in crystal waste (the predominantly ammonia of L- third
Acid, amino acid and polypeptide etc.) it is more, the direct discharge of waste liquid can not only waste the unrecovered ALANINE in part, while can be right
Environment is polluted.Therefore develop a kind of to ALANINE extraction process, to improve the recovery rate of ALANINE, reduce environmental protection pressure
Power, has important practical significance.
The content of the invention
The invention provides a kind of extracting method of ALANINE.
The invention provides following scheme：
A kind of extracting method of ALANINE, this includes：
The ALANINE zymotic fluid for being heated to 53~57 DEG C is taken, in order to adding first in the ALANINE zymotic fluid
Flocculant, the second flocculant and the 3rd flocculant are flocculated, to adding 280 in the ALANINE zymotic fluid after the flocculation
~310 milligrams of activated carbons carry out decolouring 27~32 minutes；
ALANINE zymotic fluid after the decolouring is stirred, precipitation process, by it is described through stirring, precipitation process
ALANINE zymotic fluid afterwards is separated in being put into vacuum drum filter plant, obtains flocculation clarified solution；
By it is described flocculation clarified solution temperature be 73~77 DEG C at be concentrated into clarified solution in dry matter content be 58%~
After 62%, cool the temperature to 20 DEG C and crystallized；Crystalline solid is separated with crystalline mother solution after terminating；The crystalline solid that will be obtained
Temperature be 57~63 DEG C at dried both the ALANINE.
Preferably：It is 10%~20% that ALANINE content is obtained in the ALANINE zymotic fluid.
Preferably：It is 14%~16% that ALANINE content is obtained in the ALANINE zymotic fluid.
Preferably：First flocculant is polymeric aluminum chlorides solution, and second flocculant is chitosan solution, described
3rd flocculant is polyacrylamide solution.
Preferably：The concentration of aluminium polychloride is 9%~11%, the chitosan solution in the polymeric aluminum chlorides solution
Middle chitosan concentration is 0.8%~1.1%, and concentration of polyacrylamide is 0.1~0.3% in the polyacrylamide solution.
Preferably：To the first flocculant, the second flocculant that are added in the ALANINE zymotic fluid and the 3rd flocculation
Agent is respectively with the mass ratio of the ALANINE zymotic fluid：2：50000~3：50000、1：50000~2：50000 and 1：
50000~2：50000.
Preferably：The material of precoated shet is perlite and diatomite in the vacuum drum filter plant, and vacuum pressure is
0.04~0.05 MPa；Feed pressure vacuum is 0.06~0.07 MPa；Scraper feed distance for 0.075~0.085 centimetre/
Minute.
Preferably：The vacuum pressure is 0.04 MPa；Feed pressure vacuum is 0.06 MPa；Scraper feed distance is
0.078 cm per minute.
Preferably：It is described to take the ALANINE zymotic fluid for being heated to 53~57 DEG C, including：
Take the ALANINE zymotic fluid for being heated to 55 DEG C.
Preferably：It is described by it is described flocculation clarified solution temperature be 73~77 DEG C at be concentrated into clarified solution in dry matter content
After 58%~62%, cool the temperature to 20 DEG C and crystallized；Crystalline solid is separated with liquid after terminating；The knot that will be obtained
Crystal temperature be 57~63 DEG C at dried both the ALANINE, including：
After dry matter content is 60% in being concentrated into clarified solution at being 75 DEG C in temperature by the flocculation clarified solution, by temperature
20 DEG C are down to be crystallized；Crystalline solid is separated with liquid after terminating；Enter at being 60 DEG C in temperature by the crystalline solid of acquisition
Row drying both the ALANINE.
According to the specific embodiment that the present invention is provided, the invention discloses following technique effect：
By the invention it is possible to realize a kind of extracting method of ALANINE, under a kind of implementation, this includes：Take
53~57 DEG C of ALANINE zymotic fluid is heated to, in order to adding the first flocculant, in the ALANINE zymotic fluid
Two flocculants and the 3rd flocculant are flocculated, to 280~310 milligrams of addition in the ALANINE zymotic fluid after the flocculation
Activated carbon carries out decolouring 27~32 minutes；ALANINE zymotic fluid after the decolouring is stirred, precipitation process, will be described
Separated in the ALANINE zymotic fluid after stirring, precipitation process is put into vacuum drum filter plant, obtained flocculation clear
Clear liquid；Dry matter content is 58%~62% in being concentrated into clarified solution at being 73~77 DEG C in temperature by the flocculation clarified solution
Afterwards, 20 DEG C are cooled the temperature to be crystallized；Crystalline solid is separated with crystalline mother solution after terminating；The crystalline solid that will be obtained is in temperature
Spend for dried at 57~63 DEG C both the ALANINE.The method that the application is provided is using flocculence precipitation removal fermentation
The impurity such as thalline, protein, phosphate, residual sugar are obtained in liquid, calcium precipitation in the prior art are eliminated and is crossed the operations such as film removal of impurities,
Extraction process is enormously simplify, equipment investment is reduced, and improve product quality.Using crystalline mother solution and washing brilliant residual water again
Return to flocculation, decolouring, concentrate drying circulation in.Loss of material is avoided, environmental protection pressure is reduced, yield, energy-conserving and environment-protective is improved.
Certainly, implement any product of the invention to it is not absolutely required to while reaching all the above advantage.
Specific embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained, belongs to the scope of protection of the invention.
A kind of extracting method of ALANINE is the embodiment of the invention provides, the method includes：
The ALANINE zymotic fluid for being heated to 53~57 DEG C is taken, in order to adding first in the ALANINE zymotic fluid
Flocculant, the second flocculant and the 3rd flocculant are flocculated, to adding 280 in the ALANINE zymotic fluid after the flocculation
~310 milligrams of activated carbons carry out decolouring 27~32 minutes；In actual applications, it is necessary to select the ALANINE of suitable concn to send out
Zymotic fluid, specifically, the embodiment of the present application can provide in the ALANINE zymotic fluid ALANINE content be 10%~
20%.Further, it is 14%~16% that ALANINE content is obtained in the ALANINE zymotic fluid.Specifically chosen flocculant again
When, first flocculant is polymeric aluminum chlorides solution, and second flocculant is chitosan solution, and the 3rd flocculant is
Polyacrylamide solution.Further, the concentration of aluminium polychloride is 9%~11% in the polymeric aluminum chlorides solution, described
Chitosan concentration is 0.8%~1.1% in chitosan solution, and concentration of polyacrylamide is 0.1 in the polyacrylamide solution
~0.3%.Further, to the first flocculant, the second flocculant and the 3rd added in the ALANINE zymotic fluid
Flocculant is respectively with the mass ratio of the ALANINE zymotic fluid：2：50000~3：50000、1：50000~2：50000 with
And 1：50000~2：50000.It should be noted that when above-mentioned flocculant is sequentially added, it is necessary to be carried out when flocculant is added
Stirring.
ALANINE zymotic fluid after the decolouring is stirred, precipitation process, by it is described through stirring, precipitation process
ALANINE zymotic fluid afterwards is separated in being put into vacuum drum filter plant, obtains flocculation clarified solution；It is specifically chosen again to be somebody's turn to do
During vacuum drum filter plant, the material of precoated shet is perlite and diatomite, vacuum pressure in the vacuum drum filter plant
Power is 0.04~0.05 MPa；Feed pressure vacuum is 0.06~0.07 MPa；Scraper feed distance is 0.075~0.085
Cm per minute.
By it is described flocculation clarified solution temperature be 73~77 DEG C at be concentrated into clarified solution in dry matter content be 58%~
After 62%, cool the temperature to 20 DEG C and crystallized；Crystalline solid is separated with liquid after terminating；The crystalline solid that will be obtained is in temperature
Spend for dried at 57~63 DEG C both the ALANINE.In concrete operations, can by remaining liquid after concentration with
And crystallization Liquid Residue body weight proceeds flocculation decoloration and re-starts concentration.The rate of recovery can so be greatly improved.
The method that the application is provided obtains thalline, protein, phosphate, residual sugar etc. in removing zymotic fluid using flocculence precipitation
Impurity, eliminates calcium precipitation in the prior art and crosses the operations such as film removal of impurities, enormously simplify extraction process, reduces equipment and throws
Enter, and improve product quality.Using crystalline mother solution and wash brilliant residual water come back to flocculation, decolouring, concentrate drying circulation in.
Loss of material is avoided, environmental protection pressure is reduced, yield, energy-conserving and environment-protective is improved.
The method that application is provided is described in detail below by specific embodiment
Embodiment 1
Take 500mlL and be heated to ALANINE zymotic fluid that 53 DEG C of ALANINE content is 10% in clean dry burning
In cup, added while stirring to the polymeric aluminum chlorides solution that 20 milligrams of concentration of addition in the ALANINE zymotic fluid are 9%, plus
The chitosan solution for adding 10 milligrams of concentration to be 0.8% after entering to finish stirring 5 minutes is added while stirring, and addition finishes stirring 5
10 milligrams of concentration of addition are 0.1% polyacrylamide solution after minute, and addition finishes stirring 5 minutes and stands 10 minutes.To
300 milligrams of activated carbons are added in ALANINE zymotic fluid after the flocculation decolourize 30 minutes；
ALANINE zymotic fluid after the decolouring is stirred, precipitation process, by it is described through stirring, precipitation process
ALANINE zymotic fluid afterwards is separated in being put into vacuum drum filter plant, obtains flocculation clarified solution；The vacuum drum
The material of precoated shet is perlite and diatomite in filter plant, and vacuum pressure is 0.04 MPa；Feed pressure vacuum is
0.06 MPa；Scraper feed distance is 0.078 cm per minute.490ml flocculation clarified solutions are obtained, wherein thallus removing rate is
99.28%, protein removal rate is 83.82%, and residual sugar clearance is 39.60%, light transmittance 86%, and ALANINE loss late is
1.88%；
After dry matter content is 58% in being concentrated into clarified solution at being 75 DEG C in temperature by the flocculation clarified solution, by temperature
20 DEG C are down to be crystallized；Crystalline solid is separated with liquid after terminating；Adding deionized water carries out washing crystalline substance, the knot that will be obtained
Crystal temperature be 57 DEG C at dried both the ALANINE.By ALANINE crystalline mother solution and brilliant residual can also be washed
Water reenters flocculation, precipitation, activated carbon decolorizing, in concentrate drying circulation, improves the rate of recovery.Embodiment 1 is obtained into quality
It is 72.6 grams to measure, yield 97%, and product quality meets national standard.
Embodiment 2
Take 500mlL and be heated to ALANINE zymotic fluid that 55 DEG C of ALANINE content is 15% in clean dry burning
In cup, added while stirring to the polymeric aluminum chlorides solution that 20 milligrams of concentration of addition in the ALANINE zymotic fluid are 10%,
Addition finishes the chitosan solution that 10 milligrams of concentration of addition after stirring 5 minutes are 0.1% and adds while stirring, and addition finishes stirring
10 milligrams of concentration of addition are 0.1% polyacrylamide solution after 5 minutes, and addition finishes stirring 5 minutes and stands 10 minutes.To
300 milligrams of activated carbons are added in ALANINE zymotic fluid after the flocculation decolourize 30 minutes；
ALANINE zymotic fluid after the decolouring is stirred, precipitation process, by it is described through stirring, precipitation process
ALANINE zymotic fluid afterwards is separated in being put into vacuum drum filter plant, obtains flocculation clarified solution；The vacuum drum
The material of precoated shet is perlite and diatomite in filter plant, and vacuum pressure is 0.04 MPa；Feed pressure vacuum is
0.06 MPa；Scraper feed distance is 0.075 cm per minute.490ml flocculation clarified solutions are obtained, wherein thallus removing rate is
99.48%, protein removal rate is 84.42%, and residual sugar clearance is 39.80%, light transmittance 86%, and ALANINE loss late is
1.88%；
After dry matter content is 60% in being concentrated into clarified solution at being 75 DEG C in temperature by the flocculation clarified solution, by temperature
20 DEG C are down to be crystallized；Crystalline solid is separated with liquid after terminating；Adding deionized water carries out washing crystalline substance, the knot that will be obtained
Crystal temperature be 60 DEG C at dried both the ALANINE.By ALANINE crystalline mother solution and brilliant residual can also be washed
Water reenters flocculation, precipitation, activated carbon decolorizing, in concentrate drying circulation, improves the rate of recovery.Embodiment 2 is obtained into quality
73.6 grams of amount, yield 98%, product quality meets national standard.
Embodiment 3
Take 500mlL and be heated to ALANINE zymotic fluid that 57 DEG C of ALANINE content is 20% in clean dry burning
In cup, added while stirring to the polymeric aluminum chlorides solution that 25 milligrams of concentration of addition in the ALANINE zymotic fluid are 11%,
Addition finishes the chitosan solution that 10 milligrams of concentration of addition after stirring 5 minutes are 1.1% and adds while stirring, and addition finishes stirring
10 milligrams of concentration of addition are 0.1% polyacrylamide solution after 5 minutes, and addition finishes stirring 5 minutes and stands 10 minutes.To
300 milligrams of activated carbons are added in ALANINE zymotic fluid after the flocculation decolourize 30 minutes；
ALANINE zymotic fluid after the decolouring is stirred, precipitation process, by it is described through stirring, precipitation process
ALANINE zymotic fluid afterwards is separated in being put into vacuum drum filter plant, obtains flocculation clarified solution；The vacuum drum
The material of precoated shet is perlite and diatomite in filter plant, and vacuum pressure is 0.05 MPa；Feed pressure vacuum is
0.07 MPa；Scraper feed distance is 0.085 cm per minute.475ml flocculation clarified solutions are obtained, wherein thallus removing rate is
97.48%, protein removal rate is 83.52%, and residual sugar clearance is 38.90%, light transmittance 84%, and ALANINE loss late is
1.98%；
After dry matter content is 62% in being concentrated into clarified solution at being 77 DEG C in temperature by the flocculation clarified solution, by temperature
20 DEG C are down to be crystallized；Crystalline solid is separated with liquid after terminating；Adding deionized water carries out washing crystalline substance, the knot that will be obtained
Crystal temperature be 63 DEG C at dried both the ALANINE.By ALANINE crystalline mother solution and brilliant residual can also be washed
Water reenters flocculation, precipitation, activated carbon decolorizing, in concentrate drying circulation, improves the rate of recovery.Embodiment 3 is obtained into quality
71.6 grams of amount, yield 95.8%, product quality meets national standard.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposited between operating
In any this actual relation or order.And, term " including ", "comprising" or its any other variant be intended to
Nonexcludability is included, so that process, method, article or equipment including a series of key elements not only will including those
Element, but also other key elements including being not expressly set out, or also include being this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element limited by sentence "including a ...", it is not excluded that
Also there is other identical element in process, method, article or equipment including the key element.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the scope of the present invention.It is all
Any modification, equivalent substitution and improvements made within the spirit and principles in the present invention etc., are all contained in protection scope of the present invention
It is interior.
Claims (10)
1. a kind of extracting method of ALANINE, it is characterised in that methods described includes：
The ALANINE zymotic fluid for being heated to 53~57 DEG C is taken, is flocculated to addition first in the ALANINE zymotic fluid in order
Agent, the second flocculant and the 3rd flocculant are flocculated, in the ALANINE zymotic fluid after the flocculation add 280~
310 milligrams of activated carbons carry out decolouring 27~32 minutes；
ALANINE zymotic fluid after the decolouring is stirred, precipitation process, by described by stirring, after precipitation process
ALANINE zymotic fluid is separated in being put into vacuum drum filter plant, obtains flocculation clarified solution；
After dry matter content is 58%~62% in being concentrated into clarified solution at being 73~77 DEG C in temperature by the flocculation clarified solution,
20 DEG C are cooled the temperature to be crystallized；Crystalline solid is separated with crystalline mother solution after terminating；The crystalline solid that will be obtained is in temperature
For dried at 57~63 DEG C both the ALANINE.
2. method according to claim 1, it is characterised in that in the ALANINE zymotic fluid ALANINE content is
10%~20%.
3. method according to claim 2, it is characterised in that in the ALANINE zymotic fluid ALANINE content is
14%~16%.
4. method according to claim 1, it is characterised in that first flocculant is polymeric aluminum chlorides solution, described
Second flocculant is chitosan solution, and the 3rd flocculant is polyacrylamide solution.
5. method according to claim 4, it is characterised in that the concentration of aluminium polychloride in the polymeric aluminum chlorides solution
It is 9%~11%, chitosan concentration is 0.8%~1.1% in the chitosan solution, poly- third in the polyacrylamide solution
Acrylamide concentration is 0.1~0.3%.
6. method according to claim 5, it is characterised in that to the first flocculation added in the ALANINE zymotic fluid
Agent, the second flocculant and the 3rd flocculant are respectively with the mass ratio of the ALANINE zymotic fluid：2：50000~3：
50000、1：50000~2：50000 and 1：50000~2：50000.
7. method according to claim 1, it is characterised in that the material of precoated shet is in the vacuum drum filter plant
Perlite and diatomite, vacuum pressure are 0.04~0.05 MPa；Feed pressure vacuum is 0.06~0.07 MPa；Scraper enters
Knife distance is 0.075~0.085 cm per minute.
8. method according to claim 7, it is characterised in that the vacuum pressure is 0.04 MPa；Feed pressure vacuum
Spend is 0.06 MPa；Scraper feed distance is 0.078 cm per minute.
9. the method according to any one of claim 1 to 8, it is characterised in that described to take the L- third for being heated to 53~57 DEG C
Propylhomoserin zymotic fluid, including：
Take the ALANINE zymotic fluid for being heated to 55 DEG C.
10. the method according to any one of claim 1 to 8, it is characterised in that it is described by the flocculation clarified solution in temperature
It is after 58%~62%, to cool the temperature to 20 DEG C and crystallized to be concentrated into dry matter content in clarified solution at 73~77 DEG C；Knot
Crystalline substance separates crystalline solid with liquid after terminating；By the crystalline solid of acquisition temperature be 57~63 DEG C at dried both the L-
Alanine, including：
After dry matter content is 60% in being concentrated into clarified solution at being 75 DEG C in temperature by the flocculation clarified solution, cool the temperature to
20 DEG C are crystallized；Crystalline solid is separated with liquid after terminating；Dried at being 60 DEG C in temperature by the crystalline solid of acquisition
It is dry both the ALANINE.
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| CN201611020059.2A CN106748847B (en) | 2016-11-21 | 2016-11-21 | A kind of extracting method of l-Alanine |
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| CN201611020059.2A CN106748847B (en) | 2016-11-21 | 2016-11-21 | A kind of extracting method of l-Alanine |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107216264A (en) * | 2017-06-03 | 2017-09-29 | 秦皇岛华恒生物工程有限公司 | A kind of processing method of L alanine waste liquor |
| CN108484423A (en) * | 2018-07-05 | 2018-09-04 | 秦皇岛华恒生物工程有限公司 | A method of isolating and purifying l-Alanine from l-Alanine zymotic fluid |
| CN109399835A (en) * | 2018-12-24 | 2019-03-01 | 宜昌三峡制药有限公司 | A kind of method that neomycinsulphate fermented waste fluid is separated by solid-liquid separation |
| CN109776344A (en) * | 2017-11-13 | 2019-05-21 | 秦皇岛华恒生物工程有限公司 | A method of l-Alanine and lactic acid are separated using electrodialysis |
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| CN107216264A (en) * | 2017-06-03 | 2017-09-29 | 秦皇岛华恒生物工程有限公司 | A kind of processing method of L alanine waste liquor |
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| CN109399835A (en) * | 2018-12-24 | 2019-03-01 | 宜昌三峡制药有限公司 | A kind of method that neomycinsulphate fermented waste fluid is separated by solid-liquid separation |
| CN109399835B (en) * | 2018-12-24 | 2020-10-27 | 宜昌三峡制药有限公司 | Solid-liquid separation method for neomycin sulfate fermentation waste liquid |
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| CN106748847B (en) | 2018-09-28 |
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