CN109081787B - Process for extracting levodopa from mucuna - Google Patents
Process for extracting levodopa from mucuna Download PDFInfo
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- CN109081787B CN109081787B CN201811137000.0A CN201811137000A CN109081787B CN 109081787 B CN109081787 B CN 109081787B CN 201811137000 A CN201811137000 A CN 201811137000A CN 109081787 B CN109081787 B CN 109081787B
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- C07—ORGANIC CHEMISTRY
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- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/28—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
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Abstract
The invention discloses a process for extracting levodopa from velvet beans, which comprises the following steps: (1) pre-treating; (2) firstly, leaching; (3) leaching; (4) leaching; (5) concentration: mixing the first leaching solution obtained in the step (2) and the second leaching solution obtained in the step (3), adjusting the pH value of the mixed solution, standing for 24 hours, performing centrifugal separation to obtain a precipitate and a supernatant, concentrating the supernatant to obtain a levodopa crude product I, mixing the precipitate and the third leaching solution obtained in the step (4), and drying to obtain a levodopa crude product II; (6) refining to obtain refined levodopa. Compared with the method adopting the alcohol-based extracting solution, the method adopting the water-based extracting solution for extraction has the advantages of low extraction cost, high extraction rate of levodopa obtained by extraction, high purity (higher than 90 percent) and high purity (higher than 98 percent), and is low in pollution and basically free of sewage treatment problem.
Description
Technical Field
The invention relates to the technical field of Chinese herbal medicine processing, in particular to a process for extracting levodopa from velvet beans.
Background
Levodopa is a main medicine for treating paralysis agitans, and is mainly extracted from plants such as kidney beans in domestic production. Levodopa is a phenylalanine compound, is easily soluble in water, is insoluble in organic solvents such as ethanol and the like, and has stable o-diphenol hydroxyl in molecules under acidic conditions, is easy to condense under neutral and alkaline conditions, and is easy to oxidize when encountering high temperature. In the prior art, levodopa is mainly extracted by an acid-water-alcohol method, a large amount of ethanol is consumed in the process, and the heating and concentrating time is long. At present, acid water extraction is mainly used, but due to a large amount of water-soluble impurities, the purity and the extraction rate of the levodopa are greatly influenced.
The research shows that the following acid water is used for extracting the levodopa, for example, CN201410735107.0 discloses a method for extracting the levodopa from green soybeans under the assistance of microwaves, which comprises the following steps: (1) pretreatment of raw materials: crushing the impurity-removed cat beans to 20-60 meshes, and mixing the crushed cat beans according to a solid-liquid ratio of 1: 1.5 adding deionized water, then sequentially adding 0.1%, 0.01% and 0.005-0.01% by mass of acetic acid, ascorbic acid and pectinase, mixing uniformly, and soaking for 4 h; (2) microwave-assisted extraction: adding the mixture according to the solid-liquid ratio of 1: 20, and 0.2 percent by mass of sodium thiosulfate, uniformly mixing, adding the materials into a microwave extraction device with the frequency of 2450MHz and the power of 20kw, extracting for 45-50min at the extraction temperature of 55-60 ℃, discharging the materials after the microwave extraction is finished, and separating solid from liquid by a solid-liquid separator to obtain an extracting solution; (3) fine filtering and ultrafiltration; (4) nano-filtering; (5) concentrating; (6) crystallizing; (7) separating; (8) and (5) washing and drying. Which solves the problem of environmental pollution in the traditional acid leaching extraction method. Although the problem of acid pollution of an extraction process in an acid leaching method is solved, sodium thiosulfate is added in the extraction process, and sulfur dioxide is generated to cause environmental pollution due to poor post-treatment sewage and acid reaction.
Also, for example, patent CN201611023242.8 discloses a method for extracting levodopa from velvet bean, comprising the steps of flattening the velvet bean, placing the squashed velvet bean into a reaction kettle in which an aqueous solution of ammonium bicarbonate, ammonium sulfate, tannic acid and sodium metabisulfite is dissolved, pressurizing with carbon dioxide, heating and leaching to obtain an extracting solution and velvet bean dregs; precipitating the extractive solution with chitosan, filtering, primarily separating impurities, concentrating the filtrate under reduced pressure, and separating out crude product; and leaching the crude product with an ethanol solution, recovering the ethanol after leaching, taking a filter cake, purifying the filter cake by adopting a traditional refining process, standing, separating out crystals, filtering to obtain the crystals, and drying to obtain the levodopa with the content of not less than 99% and the yield of not less than 3%. The method can improve the product yield from 2.5 percent to 3 percent, reduce the discharge amount of the acid-alkali containing wastewater by over 95 percent and save 60 percent of labor force. However, the extracting solution is extracted by taking ammonium bicarbonate, ammonium sulfate, tannic acid and sodium metabisulfite as solvents, and when acid-base fertilizer water is discharged, the nitrogen-ammonia content is increased, so that the difficulty of sewage treatment is increased.
Disclosure of Invention
The invention overcomes the technical problems of low purity, low extraction rate and serious pollution of levodopa extracted by an extraction process for extracting levodopa by acid water in the prior art, and provides a process for preparing levodopa by using velvet beans.
In order to solve the problems, the invention adopts the following technical scheme:
a process for extracting levodopa from velvet beans comprises the following steps:
(1) pretreatment: cleaning velvet beans, removing impurities, crushing and sieving with a 80-mesh sieve for later use;
(2) leaching: putting mucuna into the extracting solution, performing ultrasonic treatment for 40-50 min by adopting ultrasonic waves, and then soaking for 8h and filtering to obtain primary soybean soaking residues and primary leaching solution;
(3) leaching: soaking the bean dregs obtained in the step (1) in an extracting solution again, performing ultrasonic treatment for 40-50 min by adopting ultrasonic waves, soaking for 8h, and filtering to obtain secondary-soaked bean dregs and secondary-soaked leaching solution;
(4) leaching: soaking the second-soaked bean dregs in the extracting solution, performing ultrasonic treatment for 40-50 min by adopting ultrasonic waves, soaking for 4h, and filtering to obtain third-soaked bean dregs and a third-soaked leaching solution;
(5) concentration: mixing the first leaching solution obtained in the step (2) and the second leaching solution obtained in the step (3), adjusting the pH value of the mixed solution to 4.5-5.0, standing for 24 hours, performing centrifugal separation to obtain a precipitate and a supernatant, concentrating the supernatant to obtain a levodopa crude product I, mixing the precipitate and the third leaching solution obtained in the step (4), and drying to obtain a levodopa crude product II;
(6) refining: dissolving the levodopa crude product I and the levodopa crude product II in an aqueous solution with the mass being 3-5 times and the pH value being 3.5, adding the levodopa crude product I and vitamin C accounting for 0.1-0.5% of the total mass of the levodopa crude product II, heating to boil, filtering, adding 5-10% of activated carbon into the filtrate, stirring, preserving heat at 80-90 ℃ for 30-40 min, filtering to remove the activated carbon, and performing spray drying to obtain a levodopa fine product;
the extracting solutions in the step (1), the step (2) and the step (3) are all composed of the following components: the extraction liquid contains water 3-8 times of the mass of the velvet bean, 0.05-0.2% of vitamin C, 0.05-0.2% of acetic acid, 0.01-0.05% of protease, 0.01-0.05% of cellulase, 0.01-0.05% of sodium citrate and 0.01-0.05% of sodium metasilicate pentahydrate by mass of the velvet bean.
Wherein in the step (6), the concentration mode is decompression concentration, the pressure is 4.4-5.5 mpa, and the temperature is 30-40 ℃.
Wherein the power of the ultrasonic wave in the step (2) is 500W, and the irradiation temperature of the ultrasonic wave is 30-35 ℃.
Wherein the power of the ultrasonic wave in the step (3) is 500W, and the irradiation temperature of the ultrasonic wave is 40-45 ℃.
Wherein the power of the ultrasonic wave in the step (4) is 500W, and the irradiation temperature of the ultrasonic wave is 50-55 ℃.
Wherein in the leaching processes in the step (1), the step (2) and the step (3), the leaching temperature is 40-50 min.
Wherein, in the step (6), the spray drying conditions are that the air inlet temperature is 160 ℃, the air exhaust temperature is 90 ℃ and the spray pressure is 0.8 mpa.
The protease is composed of subtilisin and papain with the mass ratio of 1: 3-7, and the enzyme activity of the protease is not lower than 885.7U/g.
Compared with the prior art, the invention has the following beneficial effects:
(1) compared with the method adopting the alcohol-based extracting solution, the method adopting the water-based extracting solution for extraction has the advantages of low extraction cost, high extraction rate of levodopa obtained by extraction, high purity (higher than 90 percent) and high purity (higher than 98 percent), and is low in pollution and basically free of sewage treatment problem. The extracting solution disclosed by the invention consists of vitamin C, acetic acid, protease, cellulase, sodium citrate and sodium metasilicate pentahydrate, the cellulase can degrade mucuna cellulose, destroy plant cell walls and promote dissolution of levodopa, the subtilisin and the papain can degrade proteins, levodopa protein precursors in mucuna can be degraded into levodopa, and the levodopa protein precursors can be dissolved in the water-based extracting solution of the acetic acid, the vitamin C, the sodium citrate and the sodium metasilicate pentahydrate at high concentration, so that the obtained purity is high, and the extraction rate is high.
(2) According to the method, the levodopa is extracted by ultrasonic assistance, primary extraction is carried out at 30-35 ℃, the velvet beans can be efficiently enzymolyzed by the subtilisin, the papain and the cellulase at the temperature, the levodopa is quickly dissolved out under the cavitation action of the ultrasonic, the enzyme can also quickly permeate into the velvet beans, and the enzymolysis speed is greatly increased. The secondary leaching is carried out at the temperature of 40-45 ℃, the stable dissolution of the levodopa is facilitated at the temperature, the tertiary leaching is carried out at the temperature of 50-55 ℃, the enzyme is inactivated at the temperature, and the levodopa precursor is effectively dissolved out to generate the levodopa.
Detailed Description
The present invention will be further described with reference to examples and tests.
Example 1
A process for extracting levodopa from velvet beans comprises the following steps:
(1) pretreatment: cleaning velvet beans, removing impurities, crushing and sieving with a 80-mesh sieve for later use;
(2) leaching: placing mucuna in the extracting solution, performing ultrasonic treatment for 50min by adopting ultrasonic waves, soaking for 8h, and filtering to obtain primary soybean soaking residues and primary leaching solution; the power of ultrasonic waves is 500W, and the irradiation temperature of the ultrasonic waves is 30 ℃; the leaching temperature is 50 min;
(3) leaching: soaking the bean dregs obtained in the step (1) in the extracting solution again, and soaking for 8 hours after ultrasonic treatment is carried out for 40 minutes to obtain secondary-soaking bean dregs and secondary-soaking leachate; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 45 ℃; the leaching temperature is 40 min;
(4) leaching: soaking the second-soaked bean dregs in the extracting solution, performing ultrasonic treatment for 50min by adopting ultrasonic waves, soaking for 4h, and filtering to obtain third-soaked bean dregs and a third-soaked leaching solution; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 50 ℃; the leaching temperature is 50 min;
(5) concentration: mixing the first leaching solution obtained in the step (2) and the second leaching solution obtained in the step (3), adjusting the pH value of the mixed solution to 4.5, standing for 24 hours, performing centrifugal separation to obtain a precipitate and a supernatant, concentrating the supernatant to obtain a crude levodopa product I, mixing the precipitate and the third leaching solution obtained in the step (4), and drying to obtain a crude levodopa product II;
(6) refining: dissolving the levodopa crude product I and the levodopa crude product II in an aqueous solution with the mass 5 times and the pH value of 3.5, adding 0.1% of vitamin C in the total mass of the levodopa crude product I and the levodopa crude product II, heating to boil, filtering, adding 10% of active carbon into the filtrate, stirring, preserving the temperature at 80 ℃ for 40min, filtering to remove the active carbon, and performing spray drying to obtain a levodopa fine product; the concentration mode is reduced pressure concentration, the pressure is 4.4mpa, and the temperature is 40 ℃; the spray drying conditions are that the air inlet temperature is 160 ℃, the air exhaust temperature is 90 ℃ and the spray pressure is 0.8 mpa.
Wherein the extracting solutions in the step (1), the step (2) and the step (3) are all composed of the following components: based on the mass of the velvet beans, the extracting solution contains 8 times of the mass of the velvet beans of water, 0.05% of vitamin C, 0.2% of acetic acid, 0.01% of protease, 0.05% of cellulase, 0.01% of sodium citrate and 0.05% of sodium metasilicate pentahydrate. The protease is composed of subtilisin and papain with the mass ratio of 1: 3. The enzyme activity of the protease is 980.2U/g.
Example 2
A process for extracting levodopa from velvet beans comprises the following steps:
(1) pretreatment: cleaning velvet beans, removing impurities, crushing and sieving with a 80-mesh sieve for later use;
(2) leaching: placing mucuna in the extracting solution, performing ultrasonic treatment for 40min by adopting ultrasonic waves, soaking for 8h, and filtering to obtain primary soybean soaking residues and primary leaching solution; the power of ultrasonic waves is 500W, and the irradiation temperature of the ultrasonic waves is 35 ℃; the leaching temperature is 40 min;
(3) leaching: soaking the bean dregs obtained in the step (1) in the extracting solution again, and soaking for 8 hours after ultrasonic treatment is carried out for 50 minutes to obtain secondary-soaking bean dregs and secondary-soaking leachate; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 40 ℃; the leaching temperature is 40 min;
(4) leaching: soaking the second-soaked bean dregs in the extracting solution, performing ultrasonic treatment for 50min by adopting ultrasonic waves, soaking for 4h, and filtering to obtain third-soaked bean dregs and a third-soaked leaching solution; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 50 ℃; the leaching temperature is 50 min;
(5) concentration: mixing the first leaching solution obtained in the step (2) and the second leaching solution obtained in the step (3), adjusting the pH value of the mixed solution to 4.5, standing for 24 hours, performing centrifugal separation to obtain a precipitate and a supernatant, concentrating the supernatant to obtain a crude levodopa product I, mixing the precipitate and the third leaching solution obtained in the step (4), and drying to obtain a crude levodopa product II;
(6) refining: dissolving the levodopa crude product I and the levodopa crude product II in an aqueous solution with the mass 5 times and the pH value of 3.5, adding 0.1% of vitamin C in the total mass of the levodopa crude product I and the levodopa crude product II, heating to boil, filtering, adding 10% of active carbon into the filtrate, stirring, preserving the temperature at 80 ℃ for 40min, filtering to remove the active carbon, and performing spray drying to obtain a levodopa fine product; the concentration mode is reduced pressure concentration, the pressure is 4.4mpa, and the temperature is 40 ℃; the spray drying conditions are that the air inlet temperature is 160 ℃, the air exhaust temperature is 90 ℃ and the spray pressure is 0.8 mpa.
Wherein the extracting solutions in the step (1), the step (2) and the step (3) are all composed of the following components: the extract contains water 3 times of the weight of the velvet bean, vitamin C0.2%, acetic acid 0.05%, protease 0.05%, cellulase 0.01%, sodium citrate 0.05%, and sodium metasilicate pentahydrate 0.01%. The protease consists of subtilisin and papain in a mass ratio of 1: 7. The enzyme activity of the protease is 890.0U/g.
Example 3
A process for extracting levodopa from velvet beans comprises the following steps:
(1) pretreatment: cleaning velvet beans, removing impurities, crushing and sieving with a 80-mesh sieve for later use;
(2) leaching: placing mucuna in the extracting solution, performing ultrasonic treatment for 42min by adopting ultrasonic waves, soaking for 8h, and filtering to obtain primary soybean soaking residues and primary leaching solution; the power of ultrasonic waves is 500W, and the irradiation temperature of the ultrasonic waves is 32 ℃; the leaching temperature is 45 min;
(3) leaching: soaking the bean dregs obtained in the step (1) in the extracting solution again, and soaking for 8 hours after ultrasonic treatment is carried out for 48 minutes to obtain secondary-soaking bean dregs and secondary-soaking leachate; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 42 ℃; the leaching temperature is 48 min;
(4) leaching: soaking the second-soaked bean dregs in the extracting solution, performing ultrasonic treatment for 45min by adopting ultrasonic waves, soaking for 4h, and filtering to obtain third-soaked bean dregs and a third-soaked leaching solution; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 52 ℃; the leaching temperature is 45 min;
(5) concentration: mixing the first leaching solution obtained in the step (2) and the second leaching solution obtained in the step (3), adjusting the pH value of the mixed solution to 4.8, standing for 24 hours, performing centrifugal separation to obtain a precipitate and a supernatant, concentrating the supernatant to obtain a crude levodopa product I, mixing the precipitate and the third leaching solution obtained in the step (4), and drying to obtain a crude levodopa product II;
(6) refining: dissolving the levodopa crude product I and the levodopa crude product II in an aqueous solution with the mass being 4 times and the pH value being 3.5, adding 0.3% of vitamin C in the total mass of the levodopa crude product I and the levodopa crude product II, heating to boiling, filtering, adding 8% of active carbon into the filtrate, stirring, preserving the temperature at 85 ℃ for 35min, filtering to remove the active carbon, and performing spray drying to obtain a levodopa fine product; the concentration mode is reduced pressure concentration, the pressure is 5.0mpa, and the temperature is 35 ℃; the spray drying conditions are that the air inlet temperature is 160 ℃, the air exhaust temperature is 90 ℃ and the spray pressure is 0.8 mpa.
Wherein the extracting solutions in the step (1), the step (2) and the step (3) are all composed of the following components: the extractive solution contains water 6 times of the weight of the velvet bean, vitamin C0.1%, acetic acid 0.1%, protease 0.03%, cellulase 0.03%, sodium citrate 0.03%, and sodium metasilicate pentahydrate 0.03%. The protease consists of subtilisin and papain in a mass ratio of 1: 5. The enzyme activity of the protease is 888.7U/g.
To illustrate the technical effect set of the present invention, the following control groups were set up:
control group 1
The extraction process of the control 1 is substantially the same as that of the levodopa of example 1, except that the extraction solution of the control 1 is: adding 10% ammonium bicarbonate, 10% ammonium sulfate, 10% tannic acid and 3% sodium pyrosulfite by weight and volume ratio according to the weight of mucuna and 10 times of water by mass based on the total volume of the added water.
Control group 1
The extraction process of the control 1 is substantially the same as that of the levodopa of example 1, except that the extraction solution of the control 1 is: adding 10% ammonium bicarbonate, 10% ammonium sulfate, 10% tannic acid and 3% sodium pyrosulfite by weight and volume ratio according to the weight of mucuna and 10 times of water by mass based on the total volume of the added water.
Control group 2
The extraction process of the control group 2 was substantially the same as that of the levodopa of example 1, except that the extraction solution of the control group 2 was: according to the mass of the mucuna, the weight ratio of solid to liquid is 1: 21.5 adding deionized water, and then sequentially adding 0.1%, 0.01%, 0.005-0.01% and 0.2% by mass of acetic acid, ascorbic acid, pectinase and sodium thiosulfate.
Control group 3
The extraction process of control 3 was substantially the same as that of example 1 except that the extract of control 3 did not contain vitamin C.
Control group 4
The extraction process of control 4 was substantially the same as that of example 1 except that the extract of control 4 contained no cellulase.
Control group 5
The extraction process of control 5 was substantially the same as that of example 1 except that the extract of control 5 did not contain sodium citrate.
Control group 6
The extraction process of control 6 was substantially the same as that of levodopa of example 1, except that the extract of control 6 did not contain sodium metasilicate pentahydrate.
Control group 7
The extraction process of control 7 was substantially the same as that of example 1 except that the extract of control 7 contained no protease.
Control group 8
The extraction process of the control group 8 was substantially the same as that of the levodopa of example 1, except that the protease in the extract of the control group 8 was subtilisin only.
Control group 9
The extraction process of the control group 9 was substantially the same as that of the levodopa of example 1, except that the power of the ultrasonic waves in step (2), step (3) and step (4) of the control group 9 was 500W, and the irradiation temperature of the ultrasonic waves was 35 ℃.
Control group 10
The extraction process of the control group 10 was substantially the same as that of the levodopa of example 1 except that the power of the ultrasonic wave in step (2) of the control group 10 was 500W, the ultrasonic wave irradiation temperature was 55 ℃, the power of the ultrasonic wave in step (3) was 500W, the ultrasonic wave irradiation temperature was 45 ℃, the power of the ultrasonic wave in step (4) was 500W, and the ultrasonic wave irradiation temperature was 30 ℃.
Experimental detection
The extraction rate is as follows: the extraction rates of levodopa for examples 1 to 3 and controls 1 to 10 were as follows in table 1.
TABLE 1
As is clear from Table 1, the extraction yield and purity of examples 1 to 3 were slightly higher than those of control 1 and control 2, and it was found that the extract solutions used in the present invention were more effective than those of control 1 to control 2. Examples 1 to 3 showed that the extraction yield and purity were higher than those of control 3 to 8, and that the extract liquid of the present invention comprises vitamin C0.1, acetic acid, protease, cellulase, sodium citrate, sodium metasilicate pentahydrate, and water, and by combining them, the extract liquid was able to dissolve levodopa and its precursor, and the extraction yield and purity were high. The extraction rate and purity of the examples 1 to 3 are higher than those of the control group 9 and the control group 10, especially the extraction rate of the invention is high, which shows that the dissolution rate of levodopa is influenced after the ultrasonic extraction temperature is changed, and the extraction rate of levodopa is not as high as that of the invention by adopting isothermal extraction or high-temperature to low-temperature gradient extraction.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (4)
1. A process for extracting levodopa from velvet beans is characterized by comprising the following steps:
(1) pretreatment: cleaning velvet beans, removing impurities, crushing and sieving with a 80-mesh sieve for later use;
(2) leaching: putting mucuna into the extracting solution, performing ultrasonic treatment for 40-50 min by adopting ultrasonic waves, and then soaking for 8h and filtering to obtain primary soybean soaking residues and primary leaching solution; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 30-35 ℃;
(3) leaching: soaking the bean dregs obtained in the step (1) in an extracting solution again, performing ultrasonic treatment for 40-50 min by adopting ultrasonic waves, soaking for 8h, and filtering to obtain secondary-soaked bean dregs and secondary-soaked leaching solution; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 40-45 ℃;
(4) leaching: soaking the second-soaked bean dregs in the extracting solution, performing ultrasonic treatment for 40-50 min by adopting ultrasonic waves, soaking for 4h, and filtering to obtain third-soaked bean dregs and a third-soaked leaching solution; the power of the ultrasonic wave is 500W, and the irradiation temperature of the ultrasonic wave is 50-55 ℃;
(5) concentration: mixing the first leaching solution obtained in the step (2) and the second leaching solution obtained in the step (3), adjusting the pH value of the mixed solution to 4.5-5.0, standing for 24 hours, performing centrifugal separation to obtain a precipitate and a supernatant, concentrating the supernatant to obtain a levodopa crude product I, mixing the precipitate and the third leaching solution obtained in the step (4), and drying to obtain a levodopa crude product II;
(6) refining: dissolving the levodopa crude product I and the levodopa crude product II in an aqueous solution with the mass being 3-5 times and the pH value being 3.5, adding the levodopa crude product I and vitamin C accounting for 0.1-0.5% of the total mass of the levodopa crude product II, heating to boil, filtering, adding 5-10% of activated carbon into the filtrate, stirring, preserving heat at 80-90 ℃ for 30-40 min, filtering to remove the activated carbon, and performing spray drying to obtain a levodopa fine product;
the extracting solutions in the step (1), the step (2) and the step (3) are all composed of the following components: the mass of the velvet bean is taken as a reference, and the extracting solution contains 3-8 times of the mass of the velvet bean, 0.05-0.2% of vitamin C, 0.05-0.2% of acetic acid, 0.01-0.05% of protease, 0.01-0.05% of cellulase, 0.01-0.05% of sodium citrate and 0.01-0.05% of sodium metasilicate pentahydrate; the protease is composed of subtilisin and papain with the mass ratio of 1: 3-7.
2. The process of claim 1, wherein the levodopa is extracted from velvet bean, the process comprising: in the step (5), the concentration mode is reduced pressure concentration, the pressure is 4.4-5.5 MPa, and the temperature is 30-40 ℃.
3. The process of claim 1, wherein the levodopa is extracted from velvet bean, the process comprising: in the leaching processes in the step (1), the step (2) and the step (3), the leaching temperature is 40-50 min.
4. The process of claim 1, wherein the levodopa is extracted from velvet bean, the process comprising: in the step (6), the spray drying conditions are that the air inlet temperature is 160 ℃, the air exhaust temperature is 90 ℃ and the spray pressure is 0.8 mpa.
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Denomination of invention: A process for extracting levodopa from Li Dou Effective date of registration: 20230922 Granted publication date: 20210115 Pledgee: Guangxi Napo Rural Commercial Bank Co.,Ltd. Pledgor: NAPO KANGZHENG NATURAL PLANT EXTRACT Co.,Ltd. Registration number: Y2023980057174 |