CN109358146A - A kind of column filler that can quickly load chromatographic column - Google Patents
A kind of column filler that can quickly load chromatographic column Download PDFInfo
- Publication number
- CN109358146A CN109358146A CN201811592280.4A CN201811592280A CN109358146A CN 109358146 A CN109358146 A CN 109358146A CN 201811592280 A CN201811592280 A CN 201811592280A CN 109358146 A CN109358146 A CN 109358146A
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- Prior art keywords
- pressure
- article
- partition board
- pressure chamber
- cylinder body
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- 239000000945 filler Substances 0.000 title claims abstract description 23
- 238000005192 partition Methods 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims description 47
- 230000008676 import Effects 0.000 claims description 9
- 230000013011 mating Effects 0.000 claims description 7
- 238000012856 packing Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000000712 assembly Effects 0.000 description 6
- 238000000429 assembly Methods 0.000 description 6
- 230000005526 G1 to G0 transition Effects 0.000 description 5
- 238000000926 separation method Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 239000003463 adsorbent Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 238000007445 Chromatographic isolation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004262 preparative liquid chromatography Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The present invention relates to the column fillers that one kind can quickly load chromatographic column, belong to chromatographic column filling equipment technical field, including bracket, article-displacing partition board and pressurizing device；The article-displacing partition board is horizontally fixed on bracket；The pressurizing device includes cylinder body, piston plate and pressure rod；The piston plate, which is slidingly sealed, to be arranged in cylinder body, and cylinder body is divided into hydraulic pressure chamber and air-pressure chamber, and one end of pressure rod passes through air-pressure chamber and is fixed on piston plate, and the other end is correspondingly arranged at the top of article-displacing partition board；The hydraulic pressure chamber offers fluid-through port, and air-pressure chamber offers blow vent.The beneficial effects of the present invention are:, when pressure rod pressurization, providing pressure by hydraulic during filling, be conducive to increase pressure, and guarantee that pressure is stablized, improves filling quality；It after the completion of pressurization, drives pressure rod to be moved upwardly by by air pressure, effectively accelerates the speed that pressure rod retracts, improve packing efficiency, be conducive to promote and apply.
Description
Technical field
The present invention relates to chromatographic column filling equipment technical fields, and the dress column of chromatographic column can be quickly loaded more particularly to one kind
Device.
Background technique
Chromatographic isolation is to purify a kind of highest method of means efficiency in pharmaceutical industry, biological chemical field at present, is
One of the important means of the separation of natural products and the preparation of other high purity substances, application is more and more extensive.Chromatography is a kind of
Analysis means are separated, separation is core, therefore the chromatographic column for undertaking centrifugation is the heart of chromatographic system.
Chromatographic column is to generate pressure by pump, improves the elution speed that mobile phase passes through the intracorporal stationary phase adsorbent of column,
Quickening isolates and purifies the device of mixture.Due in mixture each component stationary phase and mobile phase solubility or adsoptivity not
Together, each component will flow through stationary phase adsorbent with different rates, to achieve the purpose that separation.
Column filler is the key equipment of preparative liquid chromatography separation, to efficiently separate sample, the dress of chromatographic column
Fill out the requirement of the continuity that must satisfy stationary phase bed, uniformity, stability and compactness.It is filled in the filling link of chromatographic column
It is extremely important for filling out skill, and column filler is exactly the special equipment researched and developed to load chromatographic column.
Chromatographic column includes column casing, anti-rebound component and two filter assemblies, and filter assemblies are mounted in column casing, traditional
During column filler filling, first stationary phase is inserted between two filter assemblies, is then pressurizeed by the pressure rod on column filler
Compacting, installs anti-rebound component, then withdraws pressure rod again after pressurization, finally remove chromatographic column, completes installation.In pressure process
In, the pressure rod on column filler generallys use hydraulic or air pressure and provides pressure, and withdraws pressure rod by identical power mode,
But according to hydraulic-driven pressure rod, then power delay is higher when pressure rod is withdrawn, and withdrawal speed is slow, leads to the dress of chromatographic column
Fill out low efficiency；Pressure rod is driven according to air pressure, then pressure is smaller when pressurizeing, and pumping efficiency is low, it cannot be guaranteed that filling quality.
In view of the above-mentioned problems, present applicant proposes solutions.
Summary of the invention
In order to solve the above technical problems, one technical scheme adopted by the invention is that: chromatography can quickly be loaded by providing one kind
The column filler of column, solution packing efficiency is low, loads ropy technical problem.
The purpose of the present invention is achieved through the following technical solutions: one kind can quickly load the column filler of chromatographic column,
It is characterized by comprising bracket, article-displacing partition board and pressurizing devices；The article-displacing partition board is horizontally fixed on bracket；The pressurization
Device includes cylinder body, piston plate and pressure rod；The piston plate, which is slidingly sealed, to be arranged in cylinder body, and cylinder body is divided into hydraulic
Room and air-pressure chamber, and one end of pressure rod passes through air-pressure chamber and is fixed on piston plate, the other end is correspondingly arranged at article-displacing partition board
Top；The hydraulic pressure chamber offers fluid-through port, and air-pressure chamber offers blow vent.
Preferably, the fluid-through port of the cylinder body is connected with gas drive liquid pump, and the air inlet of the gas drive liquid pump is communicated with gas source；
The first triple valve is connected between the gas drive liquid pump and fluid-through port, the import P of first triple valve is connected with fluid-through port, out
Mouth A is connected with the liquid outlet of gas drive liquid pump, and outlet B is connected with liquid reserve tank；The liquid reserve tank is connected with the inlet of gas drive liquid pump.
Preferably, the blow vent of the cylinder body is connected with the second triple valve；The import P and blow vent of second triple valve
It is connected, outlet A is communicated with atmosphere, and outlet B is connected with gas source.
Preferably, the gas source is compressed air hose.
Preferably, the compressed air hose is connected with third triple valve, and the import P and compressed air hose of third triple valve
It is connected, outlet A is connected with the air inlet of gas drive liquid pump, and outlet B is connected with the outlet B of the second triple valve.
Preferably, three-way magnetic valve can be used in first triple valve, the second triple valve and third triple valve.
Preferably, the side of the article-displacing partition board has opening, and the upper end of article-displacing partition board has mating surface.
Preferably, the two sides of the bracket are respectively fixed with control cabinet and locker.
Preferably, the lower section of the bracket has bottom plate, and the lower end rotation of bottom plate is provided with idler wheel.
Preferably, multiple lower supporting rods, one end of lower supporting rod and bottom plate are fixedly installed between the bottom plate and article-displacing partition board
It is fixedly connected, the other end is screwed with article-displacing partition board and connect；And the upper end of article-displacing partition board has been threadably secured multiple upper branch
Column, the upper end of upper supporting column are fixed with support plate, and the cylinder body is fixed on the supporting plate.
The beneficial effects of the present invention are:
During filling, when pressure rod pressurization, liquid is passed through to hydraulic pressure chamber by fluid-through port, the gas of air-pressure chamber from
Blow vent discharge, is moved downward by hydraulic drive pressure rod, is applied pressure to chromatographic column, is provided pressure by hydraulic, have
Conducive to increase pressure, and guarantee that pressure is stablized, improves filling quality；After the completion of pressurization, by blow vent to air-pressure chamber
It is passed through gas, hydraulic indoor liquid is discharged from fluid-through port, drives pressure rod to be moved upwardly by by air pressure, due to gas flow rate
Fastly, it can be rapidly filled with air-pressure chamber, and provide pressure for piston plate, therefore can effectively accelerate the speed of pressure rod retraction, improve dress
Efficiency is filled out, is conducive to promote and apply.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of preferred embodiment；
Fig. 2 is the pipeline connecting figure of cylinder body；
In figure: 1- cylinder body, 2- piston plate, 3- pressure rod, 4- hydraulic pressure chamber, 5- air-pressure chamber, 6- fluid-through port, 7- blow vent, 8-
Gas drive liquid pump, the first triple valve of 9-, 10- liquid reserve tank, the second triple valve of 11-, 12- third triple valve, 13- compressed air hose, 14-
Atmosphere,
20- bracket, 21- article-displacing partition board, 22- fender bracket, 23- control cabinet, 24- locker, 25- mating surface, 26- bottom plate,
27- idler wheel, 28- lower supporting rod, 29- upper supporting column, 30- support plate.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with attached drawing, to the present invention into
One step is described in detail.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts all
Other embodiments shall fall within the protection scope of the present invention.
As shown in Figure 1 and Figure 2, a kind of column filler that can quickly load chromatographic column, it is characterised in that: including bracket 20, glove
Partition 21 and pressurizing device；The article-displacing partition board 21 is horizontally fixed on bracket 20；The pressurizing device includes cylinder body 1, piston
Plate 2 and pressure rod 3；The piston plate 2 is slidingly sealed setting and is divided into hydraulic pressure chamber 4 and air-pressure chamber in cylinder body 1, and by cylinder body 1
5, and one end of pressure rod 3 passes through air-pressure chamber 5 and is fixed on piston plate 2, the other end is correspondingly arranged at the top of article-displacing partition board 21；
The hydraulic pressure chamber 4 offers fluid-through port 6, and air-pressure chamber 5 offers blow vent 7.
During filling, when pressure rod 3 pressurizes, liquid, the gas of air-pressure chamber 5 are passed through to hydraulic pressure chamber 4 by fluid-through port 6
Body is discharged from blow vent 7, is moved downward by hydraulic drive pressure rod 3, applies pressure to chromatographic column, provides pressure by hydraulic
Power is conducive to increase pressure, and guarantees that pressure is stablized, and improves filling quality；After the completion of pressurization, by blow vent 7 to
Air-pressure chamber 5 is passed through gas, and the liquid in hydraulic pressure chamber 4 is discharged from fluid-through port 6, drives pressure rod 3 to be moved upwardly by by air pressure, due to
Gas flow rate is fast, can be rapidly filled with air-pressure chamber 5, and provide pressure for piston plate 2, therefore can effectively accelerate the speed of the retraction of pressure rod 3
Degree, improves packing efficiency, is conducive to promote and apply.
The fluid-through port 6 of the cylinder body 1 is connected with gas drive liquid pump 8, and the air inlet of the gas drive liquid pump 8 is communicated with gas source；Institute
It states and is connected with the first triple valve 9 between gas drive liquid pump 8 and fluid-through port 6, import P and 6 phase of fluid-through port of first triple valve 9
Even, outlet A is connected with the liquid outlet of gas drive liquid pump 8, and outlet B is connected with liquid reserve tank 10；The liquid reserve tank 10 and gas drive liquid pump 8
Inlet is connected.
Gas drive liquid pump 8 is the structure to be linked together using major diameter gas piston and small-diameter hydraulic plunger.Two work
It fills in area ratio and generates pressure ratio, maximum output pressure can be calculated by pressure ratio.High-pressure ratio generates high outlet pressure.
The gas-powered range of gas drive liquid pump 8 is 1 to 10bar, and compressed air can be used as gas source.When gas drive liquid pump 8 works, pressure
Contracting air enters from the air inlet of gas drive liquid pump 8, and gas drive liquid pump 8 sucks liquid out of liquid reserve tank 10, and pumps out from liquid outlet, from
And enter in the hydraulic pressure chamber 4 of cylinder body 1.
The blow vent 7 of the cylinder body 1 is connected with the second triple valve 11；The import P and blow vent 7 of second triple valve 11
It is connected, outlet A is communicated with atmosphere 14, and outlet B is connected with gas source.
The gas source is compressed air hose 13, and compressed air hose 13 is connected with third triple valve 12, and third triple valve 12
Import P be connected with compressed air hose 13, outlet A is connected with the air inlet of gas drive liquid pump 8, outlet B and second triple valve 11
B is exported to be connected.
Three-way magnetic valve can be used in above-mentioned the first triple valve 9, the second triple valve 11 and third triple valve 12, can specifically adopt
Use two-position three way magnetic valve.
It is sealing sliding setting between piston plate 2 and the inner wall of cylinder body 1, can be specifically arranged by the outer circle in piston plate 2
Sealing ring seals to realize.
The side of the article-displacing partition board 21 has opening, when work, need to only be caught in chromatographic column by being open, install
It is convenient.And the upper end of article-displacing partition board 21 has mating surface 25, the flatness of mating surface 25 is less than or equal to 0.02mm, guarantees cooperation
Cooperate precisely between face 25 and support level, avoids deflecting when the piston rod extrusion piston plate 2 of pressure rod 3, to damage by pressure
Upper filter assemblies improve dress column quality, reduce rejection rate, and mating surface 25 is lower than the upper surface of article-displacing partition board 21, can reduce
Difficulty of processing.Locker 24 can be used to place tool or other sundries.
In order to adapt to the chromatographic column of different model, a transition sleeve can be increased on demand, the lower end of transition sleeve, which is resisted against, sets
In the mating surface 25 of object partition 21, application range is wider.
The lower section of the bracket 20 has bottom plate 26, and the lower end rotation of bottom plate 26 is provided with idler wheel 27, convenient for column filler
It is mobile.
Multiple lower supporting rods 28, one end of lower supporting rod 28 and bottom plate are fixedly installed between the bottom plate 26 and article-displacing partition board 21
26 are fixedly connected, and the other end is screwed with article-displacing partition board 21 and connect；And the upper end of article-displacing partition board 21 be threadably secured it is more
A upper supporting column 29, the upper end of upper supporting column 29 are fixed with support plate 30, and the cylinder body 1 is fixed in support plate 30, is not shown in the figure.
The surrounding of the bracket 20 is fixed with fender bracket 22, and fender bracket 22 can play protective action, and pressure rod 3 is avoided to pressurize
It breaks down and injures worker.
And the two sides of bracket 20 are respectively fixed with control cabinet 23 and locker 24.Gas drive liquid pump 8, the first triple valve 9, second
Triple valve 11 and third triple valve 12 can integrated installation in control cabinet 23, it is compact-sized.Locker 24 can accommodating sundries.
When work, first filter assemblies are packed into column casing；Assembled chromatographic column is hung on column filler, specifically hangs over and sets
On object partition 21；First triple valve 9, the second triple valve 11 and third triple valve 12 are in original state, at this time compressed air
Pipe 13 is communicated with gas drive liquid pump 8, and the liquid outlet of gas drive liquid pump 8 is to 4 pumping liquid of hydraulic pressure chamber, and air-pressure chamber 5 is communicated with atmosphere 14, band
Dynamic pressure rod 3 moves downward, and pressure rod 3 is withstood on filter assemblies, applies pressure to chromatographic column；
After pressure reaches design value, anti-rebound component is installed, then the first triple valve 9, the second triple valve 11 and the three or three
Port valve 12 is powered, and compressed air hose 13 communicates with air-pressure chamber 5, provides air pressure to air-pressure chamber 5, at the same the fluid-through port 6 of hydraulic pressure chamber 4 and
Liquid reserve tank 10 communicates, and under the influence of air pressure, pressure rod 3 moves up rapidly, withdraws pressure rod 3, completes chromatographic column filling.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, there can be various modifications and variations in the spirit and principles in the present invention, these equivalent variation or replacement etc.,
It is all included in the scope of protection of the present invention.
Claims (10)
1. the column filler that one kind can quickly load chromatographic column, it is characterised in that: including bracket, article-displacing partition board and pressurizing device；Institute
Article-displacing partition board is stated to be horizontally fixed on bracket；The pressurizing device includes cylinder body, piston plate and pressure rod；The piston plate sliding
It is sealingly disposed in cylinder body, and cylinder body is divided into hydraulic pressure chamber and air-pressure chamber, and one end of pressure rod is fixed on across air-pressure chamber
On piston plate, the other end is correspondingly arranged at the top of article-displacing partition board；The hydraulic pressure chamber offers fluid-through port, and air-pressure chamber offers logical
Port.
2. column filler according to claim 1, it is characterised in that: the fluid-through port of the cylinder body is connected with gas drive liquid pump, institute
The air inlet for stating gas drive liquid pump is communicated with gas source；It is connected with the first triple valve between the gas drive liquid pump and fluid-through port, described
The import P of one triple valve is connected with fluid-through port, and outlet A is connected with the liquid outlet of gas drive liquid pump, and outlet B is connected with liquid reserve tank；Institute
Liquid reserve tank is stated to be connected with the inlet of gas drive liquid pump.
3. column filler according to claim 2, it is characterised in that: the blow vent of the cylinder body is connected with the second triple valve；
The import P of second triple valve is connected with blow vent, and outlet A is communicated with atmosphere, and outlet B is connected with gas source.
4. column filler according to claim 3, it is characterised in that: the gas source is compressed air hose.
5. column filler according to claim 4, it is characterised in that: the compressed air hose is connected with third triple valve, and
The import P of third triple valve is connected with compressed air hose, and outlet A is connected with the air inlet of gas drive liquid pump, exports B and the second threeway
The outlet B of valve is connected.
6. column filler according to claim 5, it is characterised in that: first triple valve, the second triple valve and the three or three
Three-way magnetic valve can be used in port valve.
7. column filler according to claims 1 to 6, it is characterised in that: the side of the article-displacing partition board has opening, and sets
The upper end of object partition has mating surface.
8. column filler according to claim 7, it is characterised in that: the two sides of the bracket are respectively fixed with control cabinet and storage
Object cabinet.
9. column filler according to claim 7, it is characterised in that: the lower section of the bracket has bottom plate, the lower end of bottom plate
Rotation is provided with idler wheel.
10. column filler according to claim 9, it is characterised in that: be fixedly installed between the bottom plate and article-displacing partition board
One end of multiple lower supporting rods, lower supporting rod is fixedly connected with bottom plate, and the other end is screwed with article-displacing partition board and connect；And article-displacing partition board
Upper end be threadably secured multiple upper supporting columns, the upper end of upper supporting column is fixed with support plate, and the cylinder body is fixed on support plate
On.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811592280.4A CN109358146A (en) | 2018-12-25 | 2018-12-25 | A kind of column filler that can quickly load chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811592280.4A CN109358146A (en) | 2018-12-25 | 2018-12-25 | A kind of column filler that can quickly load chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109358146A true CN109358146A (en) | 2019-02-19 |
Family
ID=65330121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811592280.4A Pending CN109358146A (en) | 2018-12-25 | 2018-12-25 | A kind of column filler that can quickly load chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109358146A (en) |
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