CN109358034B - Astronaut urine detection method based on liquid core waveguide combined spectrum - Google Patents
Astronaut urine detection method based on liquid core waveguide combined spectrum Download PDFInfo
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Abstract
The invention discloses a liquid core waveguide combined spectrum-based astronaut urine detection method, which is realized on a liquid core waveguide combined spectrum-based astronaut urine detection system and comprises five steps of continuous liquid sample introduction for testing, combined spectrum test initialization, dynamic liquid core waveguide L IBS test, dynamic liquid core waveguide Raman test, real-time waste liquid collection, test completion, data post-processing, residual waste liquid post-processing and the like.
Description
Technical Field
The invention relates to a micro-area detection method, in particular to a combined spectrum micro-area detection method based on liquid core waveguide, which is suitable for daily monitoring of the urine of astronauts in the microgravity environment of a space station and belongs to the field of photoelectric detection.
Background
Manned space refers to the flying activities of people in space to and from the earth, such as driving a spacecraft to carry out various sciences, tests, researches and the like. The regions where astronauts work are mainly manned spacecrafts, space stations, extraterrestrial camps and the like. Where space stations are the most common and longer-term workplaces. In space stations, astronauts are in a long term microgravity environment and their physical condition needs to be monitored at any time in order to invent their potential health effects and problems.
Health monitoring of astronauts in a spacecraft may take many test samples. Wherein, the monitoring to the urine of the astronaut is very important and is easy to obtain. Substances monitored include creatinine, protein, urea, and the like. Creatinine in urine, for example, is a product of muscle metabolism in the human body and is primarily excreted by glomerular filtration. The creativity of creatinine can be 1mg per 20g of muscle metabolism, and when the food intake of meat is stable, the creativity of creatinine is relatively constant without great change of the muscle metabolism of the body. Urea nitrogen increased with creatinine indicating severe kidney damage. In addition, some diseases are related to the concentration of certain transition metals in human body fluids. For example, Wilson's disease may occur if the urine contains a relatively low amount of copper. By analyzing the copper content in the urine, Hodgkin disease, leukemia and other malignant diseases can be judged; the sodium urine determination can be used to determine whether salt intake is sufficient and there is a large loss of salt, and can also assist in monitoring low-salt diet and post-operative electrolyte supervision, and assist in determining electrolyte balance in patients with emesis, severe diarrhea, and heat-related aging. The kidney is the main excretory organ of sodium salt, and the function and pathological changes of the kidney can be known by measuring natrium urinaria. The increase of natrium in urine can be adrenal cortex insufficiency, renal failure, nephrotic syndrome, etc.; the decrease in natriuresis may be hyperadrenic adrenocortical function, decrease in urine volume in the late stage of chronic renal failure, or anuresis. The detection of transition metals in urine can monitor daily health and can also be used for determining certain diseases.
At present, a urine detection instrument adopts different principles including a pH value, a specific density method, an acid-base index agent, an enzyme method and the like in the detection of creatinine, protein, urea and the like in urine, and needs a large amount of reagents and the like for supporting, so that the requirement of operation and detection of astronauts cannot be met. At present, Atomic Absorption Spectroscopy (AAS) and ion chromatography are commonly adopted for detecting transition metals in urine, the detection sensitivity of the AAS method to copper is low, and a combustion system is easily blocked by body fluid; the ion chromatography needs a complex ion chromatography exchange column structure and auxiliary reagents, the device structure and the test operation are complex, and the requirement of the astronaut for operation and detection and the requirement of the astronaut environment are difficult to satisfy. Therefore, a rapid, efficient and convenient detection method suitable for astronauts and corresponding detection equipment are needed to be found for creatinine, protein, urea and transition metals so as to meet the requirement of health real-time diagnosis of the astronauts.
The laser Raman spectrum method is a convenient and feasible method for detecting creatinine, protein, urea and the like in urine, but because the content of the creatinine, the protein, the urea and the like in the urine is low, and a Raman signal is weak, the problem that the signal is weak is solved if the Raman spectrum method is used for monitoring the urine of astronauts.A laser-induced breakdown spectroscopy (L ase-induced breakdown spectroscopy, L IBS for short) method is a convenient and feasible method for detecting transition metals in the urine, but because the content of metal elements in the urine is low, a single-point single microcell L IBS signal is weak, a plurality of IBS measurement signals of a plurality of microcells L are integrated for accumulation, and the problem of detection of the transition metals in the urine is solved.
Disclosure of Invention
In view of the above requirements, the present invention provides a method for detecting creatinine, protein, urea, and transition metals in urine of an astronaut based on liquid core waveguide raman and L IBS spectrum, which can detect L IBS signals of creatinine, protein, urea, and trace metals such as copper and sodium in urine under the aerospace environment requirements in microgravity, compactness, durability, etc., thereby implementing real-time diagnosis of part of physiological indexes of the astronaut reflecting health conditions.
The invention is realized by the following steps:
the method for detecting creatinine, protein, urea and transition metal in the urine of the astronaut based on the liquid core waveguide Raman and L IBS combined spectrum is realized on a system for detecting the urine of the astronaut based on the liquid core waveguide combined spectrum, and the system mainly comprises a main controller, a collection subsystem, a L IBS subsystem, a Raman spectrum subsystem and a sample injection subsystem;
the L IBS subsystem is responsible for L IBS laser emission and L IBS signal reception, and comprises a microscope objective, a dichroic mirror, a beam expander, a L IBS laser, an optical fiber coupling mirror, a receiving optical fiber, a L IBS spectrometer and a delay controller, wherein the delay controller is provided with a control port A and a control port B which are respectively connected with an external trigger port of the spectrometer of the L IBS spectrometer and an external trigger port of the laser of the L IBS laser;
the Raman spectrum subsystem is responsible for Raman laser emission and Raman signal reception and comprises a liquid core waveguide tube, a window glass slide, a Raman objective lens, a Raman dichroic mirror, an interference optical filter, a Raman beam expanding lens, a Raman laser, a Rayleigh optical filter, a Raman fiber coupling lens, a Raman receiving fiber, a Raman spectrometer, a total reflection mirror and a seal; one end of the liquid core waveguide tube is attached to the window glass slide, the other end of the liquid core waveguide tube is attached to the total reflection mirror, and the total reflection mirror is reinforced and fixed by a seal;
the system comprises a sample introduction subsystem, a liquid core waveguide tube, a liquid inlet tube, a stepper motor, a liquid inlet tube and a sealing cover, wherein the sample introduction subsystem is used for sending a test liquid sample (such as astronaut urine) to an L IBS subsystem and a Raman spectrum subsystem for analysis, and consists of a connecting hose, a liquid inlet device, an electric piston, a stepper motor, a liquid inlet tube, a liquid inlet port and a sealing cover;
the collecting subsystem is used for collecting a test liquid sample in a microgravity environment of a space station and preventing the environment from being polluted and consists of a direct current power supply, an electric cathode, a hemispherical cover, a flow outlet pipe, an electric anode, a waste liquid box and a window glass slide, wherein the flow outlet pipe is communicated with the liquid core waveguide pipe;
l IBS laser can emit narrow pulse laser beam with certain wavelength lambda from right to left along optical main axis, after beam expansion by beam expander (diameter of laser beam is matched with entrance pupil of microscope objective), through dichroic mirror, microscope objective and window glass, can focus on test liquid sample (injection: astronaut urine) at focus test point, L IBS signal excited at focus test point is transmitted right along optical main axis, after passing through window glass and microscope objective in turn, reflected by dichroic mirror, transmitted along receiving optical axis, focused by fiber coupling mirror into receiving optical fiber, and transmitted into L IBS spectrometer for analysis;
the Raman laser can emit a certain wavelength lambda from right to left along the main axis of the Raman optics1The continuous laser beam is expanded by a Raman beam expander (the diameter of the obtained laser beam is matched with the entrance pupil of a Raman microscope objective), then passes through an interference filter to obtain a narrow-frequency Raman laser beam, then passes through a Raman dichroic mirror, passes through the Raman microscope objective and a window glass slide, and can be focused into a liquid core waveguide tube to test a liquid sample (such as astronaut urine), a Raman forward scattering signal excited at a focusing point is transmitted leftwards along the axis of the liquid core (the axis of the liquid core is completely superposed with a Raman optical main shaft) and continuously collides with molecules in the test liquid sample to obtain accumulation and reinforcement, the Raman forward scattering signal is transmitted rightwards along the axis of the liquid core after being transmitted to a total reflection mirror, and continuously collides with the molecules in the test liquid sample again through reflection, the Raman scattering signal is further reinforced, and the Raman scattering signal sequentially passes through the window glass slide and the Raman microscope and is reflected by the Raman dichroic mirror, transmitting along Raman receiving optical axis, filtering with Rayleigh filter to remove wavelength λ1The Raman scattered light after the pumping light enters a Raman receiving optical fiber through the focusing of a Raman optical fiber coupling mirror and is transmitted into a Raman spectrometer for analysis;
the main controller is used for starting and closing the direct-current power supply, the L IBS laser, the Raman laser, the delay controller, the L IBS spectrometer and the Raman spectrometer, sending a control instruction to the stepper motor, setting working parameters of the L IBS spectrometer and the Raman spectrometer, and receiving spectral data of the L IBS spectrometer and the Raman spectrometer for analysis;
the invention provides a method for detecting creatinine, protein, urea and transition metal in astronaut urine based on liquid core waveguide Raman and L IBS spectra, which comprises the following steps:
(1) continuous sample introduction of test liquid
Introducing a test liquid sample (injection: astronaut urine) into the liquid inlet device through the liquid inlet, and covering the sealing cover after the introduction is finished; the main controller sends an instruction to start the direct current power supply and then sends a control instruction to the stepper motor to drive the electric piston to slowly translate to the right in the liquid inlet device, and the test liquid sample is slowly pushed into the liquid core waveguide tube through the connecting hose and the inflow tube; the sample introduction process is continuously carried out, and the whole test process is continued until the test is finished;
(2) joint spectral test initialization
When a test liquid sample is filled in the whole liquid core waveguide tube along the axis of the liquid core, the main controller sets L IBS spectrometer working parameters, then the time delay controller is started, the time delay controller starts L IBS laser firstly according to a preset time delay value, and then starts L IBS spectrometer, and then the main controller sends an instruction to start the Raman laser and the Raman spectrometer and sets the Raman spectrometer working parameters;
(3) dynamic liquid core waveguide L IBS test
L IBS laser emits narrow pulse laser with certain wavelength lambda from right to left, expands beam by beam expander, passes through dichroic mirror, passes through microscope objective and window glass slide, focuses to test liquid sample (injection: astronaut urine) at focus test point, L IBS signal excited at focus test point is transmitted right along optical main axis, passes through window glass slide and microscope objective in turn, is reflected by dichroic mirror, transmitted along receiving optical axis, focused by fiber coupling mirror into receiving optical fiber, transmitted to L IBS spectrometer, L IBS spectrometer transmits L IBS spectrum data to main controller in real time, with continuous sampling, main controller continuously collects and stores dynamic liquid core wave L IBS spectrum data;
(4) dynamic liquid core waveguide raman testing
While the dynamic liquid core waveguide L IBS test is carried out, the Raman laser emits a certain wavelength lambda from right to left1The continuous laser beam is expanded by a Raman beam expander and then passes through an interference filter to obtain a narrow-frequency Raman laser beam, then the narrow-frequency Raman laser beam passes through a Raman dichroic mirror, passes through a Raman microscope objective and passes through a window glass, and is focused into a liquid core waveguide tube to test a liquid sample (injection: astronaut urine), a Raman forward scattering signal excited at a focusing point is transmitted leftwards along the axis of a liquid core and continuously collides with molecules in the test liquid sample to obtain accumulation and reinforcement, and is transmitted to a full-reflecting mirror, then is transmitted rightwards along the axis of the liquid core through reflection, and continuously collides with the molecules in the test liquid sample again, and the Raman scattering signal obtains a Raman scattering signalTo further enhance, the Raman micro-object passes through the window glass and the Raman micro-object lens in sequence, is reflected by the Raman dichroic mirror, is transmitted along a Raman receiving optical axis, and is filtered by a Rayleigh filter to filter out the wavelength lambda1The Raman scattering light after the pumping light enters the Raman receiving optical fiber through the focusing of the Raman fiber coupling mirror and is transmitted into the Raman spectrometer, the Raman spectrometer transmits Raman spectrum data to the main controller in real time, and the main controller continuously collects and stores the dynamic liquid core waveguide Raman spectrum data along with the continuous sampling;
(5) real-time collection of waste liquid
In the testing process, sample introduction is continuously carried out; the test liquid sample in the liquid core waveguide tube is sprayed to the electric cathode along the spraying axis through the outflow tube, so that the test liquid sample with negative charges is attracted to the electric anode under the action of an electric field and enters the waste liquid tank;
(6) test end and data post-processing
When the electric piston moves to the bottom of the right side in the liquid inlet device, the whole liquid inlet device is free of a test liquid sample, the test is finished, the main controller sends an instruction to close the delay controller, L IBS laser and L IBS spectrometer, then the main controller sends an instruction to close the Raman laser and the Raman spectrometer, the main controller carries out statistical accumulation processing on the obtained multiple groups of dynamic liquid core waveguide L IBS spectrum data to obtain L IBS signal detection of trace metals such as copper, sodium and the like in urine reflecting the health condition of a astronaut, the main controller carries out statistical accumulation and average processing on the obtained multiple groups of dynamic liquid core waveguide Raman spectrum data to obtain Raman spectrum signals reflecting the health condition of the astronaut and related to the contents of creatinine, protein and urea in the urine of the astronaut, the astronaut signal data is compared with health indexes, and the L IBS spectrum analysis result is fused with the Raman spectrum analysis result, so that the real-time diagnosis of partial physiological indexes of the astronaut is realized;
(7) post-treatment of residual waste liquid
The main controller sends a control command to the stepper motor to drive the electric piston to slowly translate leftwards in the liquid inlet device until reaching the leftmost position of the liquid inlet device; opening the sealing cover, introducing clean water into the liquid inlet device through the liquid inlet, and covering the sealing cover after the introduction is finished; the main controller sends a control instruction to the stepper motor to drive the electric piston to slowly translate to the right in the liquid inlet device, clean water is slowly pushed into the liquid core waveguide tube through the connecting hose and the inflow pipe, meanwhile, residual waste liquid in the liquid core waveguide tube is pressed into the collecting subsystem to be collected until the electric piston translates to the bottom of the right side in the liquid inlet device, at the moment, only clean water exists in the liquid core waveguide tube, the main controller sends an instruction, and the direct-current power supply is disconnected.
The method has the advantages that the method can simultaneously realize the enhancement of weak Raman signals and the accumulation of multipoint multiple microcell L IBS measurement signals by adopting a detection method based on the moving liquid core waveguide combined spectrum, enables the component detection of the urine of astronauts to be more reliable by adopting the statistical measurement of a large amount of samples, meets the requirements under the microgravity environment by adopting a direct current field method in the waste liquid collection, and meets the requirements of environmental protection and sanitation by adopting purified water to clean the liquid core at the end of the test.
Drawings
FIG. 1 is a schematic structural diagram of the system of the present invention, in which the system comprises 1-a DC power supply, 2-an electric cathode, 3-a hemispherical cover, 4-an injection axis, 5-a flow-out tube, 6-a liquid core waveguide, 7-a liquid core axis, 8-a flow-in tube, 9-a control port B, 10-a microobjective, 11-an optical spindle, 12-a dichroic mirror, 13-a laser external trigger port, 14-a beam expander, 15-L IBS laser, 16-a spectrometer external trigger port, 17-a fiber coupler, 18-a receiving fiber, 19-L IBS spectrometer, 20-a connecting hose, 21-a liquid inlet, 22-a liquid sample, 23-an electric piston, 24-a stepper motor, 25-a main controller, 26-a delay controller, 27-a control port A, 28-an electric anode, 29-a waste liquid tank, 30-a collecting subsystem, 31-L-an IBS subsystem, 32-an IBS subsystem, 33-a receiving optical axis, a Raman liquid inlet, 35-a sealing cover, 36-a focusing window, 37-a focusing optical filter point 38, a specimen, a sample inlet, a Raman optical filter, a Raman optical axis, a Raman optical filter, a Raman optical.
Detailed Description
The specific embodiment of the present invention is shown in fig. 1.
The method for detecting creatinine, protein, urea and transition metal in the urine of the astronaut based on the liquid core waveguide Raman and L IBS combined spectrum is realized on a system for detecting the urine of the astronaut based on the liquid core waveguide combined spectrum, and the system mainly comprises a main controller 25, a collection subsystem 30, a L IBS subsystem 31, a Raman spectrum subsystem 43 and a sample injection subsystem 32;
the L IBS subsystem 31 is responsible for L IBS laser emission and L IBS signal reception, and comprises a microscope objective lens 10, a dichroic mirror 12, a beam expander 14, a L IBS laser 15, a fiber coupling mirror 17, a receiving fiber 18, a L IBS spectrometer 19 and a delay controller 26, wherein the delay controller 26 is provided with a control port A27 and a control port B9 which are respectively connected with a spectrometer external trigger port 16 of the L IBS spectrometer 19 and a laser external trigger port 13 of the L IBS laser 15, and the delay controller 26 sends out pulse signals with certain delay through a control port A27 and a control port B9 so as to adjust the delay between the light emission of the L IBS laser 15 and the signal acquisition of the L IBS spectrometer 19;
the raman spectrum subsystem 43 is responsible for raman laser emission and raman signal reception, and is composed of a liquid core waveguide tube 6, a window glass 50, a raman objective 49, a raman dichroic mirror 47, an interference filter 46, a raman beam expanding lens 45, a raman laser 44, a rayleigh filter 41, a raman fiber coupling lens 40, a raman receiving fiber 39, a raman spectrometer 38, a holophote 51 and a seal 52; one end of the liquid core waveguide tube 6 is attached to the window glass slide 50, the other end is attached to the total reflection mirror 51, and the total reflection mirror 51 is reinforced and fixed by a seal 52;
the sample injection subsystem 32 is used for sending the test liquid sample 22 (for injection of the urine of the astronaut) to the L IBS subsystem 31 and the Raman spectrum subsystem 43 for analysis, the sample injection subsystem 32 consists of a connecting hose 20, a liquid inlet device 21, an electric piston 23, a stepper motor 24, a flow inlet pipe 8, a liquid inlet 34 and a sealing cover 35, the test liquid sample 22 is introduced into the liquid inlet device 21 through the liquid inlet 34, the sealing cover 35 is covered after the introduction is finished, the flow inlet pipe 8 is communicated with the liquid core waveguide tube 6, the connecting hose 20 connects the flow inlet pipe 8 with the liquid inlet device 21, the stepper motor 24 can drive the electric piston 23 to translate in the liquid inlet device 21, and the test liquid sample 22 is slowly pushed into the liquid core waveguide tube 6 through the connecting hose 20 and the flow inlet pipe 8;
the collecting subsystem 30 is used for collecting the test liquid sample 22 in a microgravity environment of a space station and preventing the environment from being polluted, the collecting subsystem 30 consists of a direct current power supply 1, an electric cathode 2, a hemispherical cover 3, an outflow pipe 5, an electric anode 28, a waste liquid box 29 and a window glass sheet 37, the outflow pipe 5 is communicated with a liquid core waveguide tube 6, the electric cathode 2 is arranged on the inner wall of the hemispherical cover 3, the inlet of the waste liquid box 29 is the electric anode 28, the positive pole and the negative pole of the direct current power supply 1 are respectively connected with the electric cathode 2 of the electric anode 28 and maintain the electric field between the electric anode 28 and the electric cathode 2, the test liquid sample 22 in the liquid core waveguide tube 6 is sprayed to the electric cathode 2 along a spraying axis 4 through the outflow pipe 5 so as to carry negative charges and is attracted to the electric anode 28 and enter the waste liquid box 29 under the action of the electric field, and the window glass sheet 37 is arranged on the;
the raman laser 44 may emit a wavelength λ from right to left along the raman optical principal axis 481Continuous laser beam (532 nm wavelength link in this example)Laser continuation), expanded by the raman beam expander 45 (note: the diameter of the obtained laser beam is matched with the entrance pupil of the raman microscope objective 49), then the narrow-band raman laser beam is obtained through the interference filter 46, then the narrow-band raman laser beam passes through the raman dichroic mirror 47, passes through the raman microscope objective 49 and passes through the window glass 50, and then the narrow-band raman laser beam can be focused into the liquid core waveguide 6 to test the liquid sample 22 (note: astronaut urine), raman forward scattering signal excited at the focus point along the liquid core axis 7 (note: the liquid core axis 7 and the raman optical spindle 48 are completely coincident) transmit leftwards and continuously collide with molecules in the test liquid sample 22, so as to be accumulated and enhanced, after transmitting to the total reflection mirror 51, the liquid core axis 7 transmits rightwards through reflection, and continuously collides with molecules in the test liquid sample 22 again, so that raman scattering signals are further enhanced, sequentially pass through the window glass 50 and the raman microscope objective 49, are reflected by the raman dichroic mirror 47, are transmitted along the raman receiving optical axis 42, and are filtered by the rayleigh filter 41 to remove the wavelength lambda of the lambda1The raman scattered light after the pumping light enters a raman receiving fiber 39 through the focusing of a raman fiber coupling mirror 40 and is transmitted into a raman spectrometer 38 for analysis;
the main controller 25 is used for starting and closing the dc power supply 1, the L IBS laser 15, the raman laser 44, the delay controller 26, the L IBS spectrometer 19 and the raman spectrometer 38, sending a control command to the stepper motor 24, setting the working parameters of the L IBS spectrometer 19 and the raman spectrometer 38, and receiving the spectral data of the L IBS spectrometer 19 and the raman spectrometer 38 for analysis;
the invention provides a method for detecting creatinine, protein, urea and transition metal in astronaut urine based on liquid core waveguide Raman and L IBS spectra, which comprises the following steps:
(1) continuous sample introduction of test liquid
Introducing a test liquid sample 22 (injection: astronaut urine) into the liquid inlet device 21 through the liquid inlet 34, and covering the sealing cover 35 after the introduction is finished; the main controller 25 sends an instruction to start the direct current power supply 1, and then sends a control instruction to the stepper motor 24 to drive the electric piston 23 to slowly translate rightward in the liquid inlet device 21, and slowly push the test liquid sample 22 into the liquid core waveguide tube 6 through the connecting hose 20 and the inflow tube 8; the sample introduction process is continuously carried out, and the whole test process is continued until the test is finished;
(2) joint spectral test initialization
When the test liquid sample 22 is filled in the whole liquid core waveguide tube 6 along the liquid core axis 7, the main controller 25 sets L IBS spectrometer 19 working parameters, then the delay controller 26 is started, the delay controller 26 starts L IBS laser 15 according to a preset delay value, then starts L IBS spectrometer 19, then the main controller 25 sends an instruction to start the Raman laser 44 and the Raman spectrometer 38, and sets the Raman spectrometer 38 working parameters;
(3) dynamic liquid core waveguide L IBS test
(4) dynamic liquid core waveguide raman testing
While the dynamic liquid core waveguide L IBS test is being performed, the Raman laser 44 emits a certain wavelength λ from right to left1The continuous laser beam is expanded by a Raman beam expander 45 and then passes through an interference filter 46 to obtain a narrow-frequency Raman laser beam, then passes through a Raman dichroic mirror 47, passes through a Raman microscope 49, passes through a window glass 50, and is focused to a test liquid sample 22 (such as astronaut urine) in a liquid core waveguide tube 6, a Raman forward scattering signal excited at a focusing point is transmitted leftwards along a liquid core axis 7 and continuously collides with molecules in the test liquid sample 22 to obtain accumulation and reinforcement, and after being transmitted to a total reflection mirror 51, the Raman forward scattering signal is transmitted rightwards along the liquid core axis 7 through reflection and continuously collides with the molecules in the test liquid sample 22 again, so that the Raman scattering laser beam is subjected to Raman scatteringThe signal is further enhanced, and after sequentially passing through the window glass 50 and the Raman microscope objective 49, the signal is reflected by the Raman dichroic mirror 47, transmitted along the Raman receiving optical axis 42, filtered by the Rayleigh filter 41 to remove the wavelength lambda1The Raman scattered light after the pumping light enters a Raman receiving optical fiber 39 through the focusing of a Raman fiber coupling mirror 40 and is transmitted into a Raman spectrometer 38, the Raman spectrometer 38 transmits Raman spectrum data to the main controller 25 in real time, and the main controller 25 continuously collects and stores the dynamic liquid core waveguide Raman spectrum data along with the continuous sampling;
(5) real-time collection of waste liquid
In the testing process, sample introduction is continuously carried out; the test liquid sample 22 in the liquid core waveguide tube 6 is sprayed to the electric cathode 2 along the spraying axis 4 through the outflow tube 5, so as to be charged with negative charges, and is attracted to the electric anode 28 and enters the waste liquid tank 29 under the action of an electric field;
(6) test end and data post-processing
When the electric piston 23 moves to the bottom of the right side in the liquid inlet device 21, the whole liquid inlet device 21 has no test liquid sample 22, the test is finished, the main controller 25 sends an instruction to close the delay controller 26, L IBS laser 15 and L IBS spectrometer 19, then the main controller 25 sends an instruction to close the Raman laser 44 and the Raman spectrometer 38, the main controller 25 carries out statistical accumulation processing on the obtained multiple groups of dynamic liquid core waveguide L IBS spectrum data to obtain L IBS signal detection of trace metals such as copper, sodium and the like in urine reflecting the health condition of a astronaut, the main controller 25 carries out statistical accumulation and average processing on the obtained multiple groups of dynamic liquid core waveguide Raman spectrum data to obtain Raman spectrum signals reflecting the health condition of the astronaut and related to the contents of creatinine, protein and urea in the urine of the astronaut, the signal data are compared with health indexes, and the IBS spectrum analysis result of L is fused with the Raman spectrum analysis result, so that the real-time diagnosis of partial physiological indexes of the astronaut is realized;
(7) post-treatment of residual waste liquid
The main controller 25 sends a control instruction to the stepper motor 24 to drive the electric piston 23 to slowly translate leftwards in the liquid inlet device 21 until reaching the leftmost position of the liquid inlet device 21; opening the sealing cover 35, leading clean water into the liquid inlet device 21 through the liquid inlet 34, and covering the sealing cover 35 after leading is completed; the main controller 25 sends a control instruction to the stepper motor 24 to drive the electric piston 23 to slowly translate rightward in the liquid inlet device 21, clean water is slowly pushed into the liquid core waveguide tube 6 through the connecting hose 20 and the inflow pipe 8, meanwhile, residual waste liquid in the liquid core waveguide tube 6 is pressed into the collection subsystem 30 to be collected, and the process is finished until the electric piston 23 translates to the bottom of the right side in the liquid inlet device 21, at the moment, only clean water exists in the liquid core waveguide tube 6, the main controller 25 sends an instruction, and the direct current power supply 1 is disconnected.
Claims (1)
1. A astronaut urine detection method based on liquid core waveguide combined spectrum is realized on an astronaut urine detection system based on liquid core waveguide combined spectrum, the system comprises a main controller (25), a collection subsystem (30), an L IBS subsystem (31), a Raman spectrum subsystem (43) and a sample injection subsystem (32), and is characterized by comprising the following steps:
1) continuous sample introduction of test liquid
Introducing a test liquid sample, namely the urine of the astronaut into a liquid inlet device through a liquid inlet, and covering a sealing cover after the introduction is finished; the main controller sends an instruction to start the direct current power supply and then sends a control instruction to the stepper motor to drive the electric piston to slowly translate to the right in the liquid inlet device, and the test liquid sample is slowly pushed into the liquid core waveguide tube through the connecting hose and the inflow tube; the sample introduction process is continuously carried out, and the whole test process is continued until the test is finished;
2) joint spectral test initialization
When a test liquid sample is filled in the whole liquid core waveguide tube along the axis of the liquid core, the main controller sets L IBS spectrometer working parameters, then the time delay controller is started, the time delay controller starts L IBS laser firstly according to a preset time delay value, and then starts L IBS spectrometer, and then the main controller sends an instruction to start the Raman laser and the Raman spectrometer and sets the Raman spectrometer working parameters;
3) dynamic liquid core waveguide L IBS test
L IBS laser emits narrow pulse laser with certain wavelength lambda from right to left, expands beam by beam expander, passes through dichroic mirror, passes through microscope objective and window glass slide, focuses to test liquid sample at focus test point, L IBS signal excited at focus test point is transmitted right along optical main axis, passes through window glass slide and microscope objective in turn, is reflected by dichroic mirror, transmitted along receiving optical axis, focused by optical fiber coupling mirror into receiving optical fiber, transmitted into L IBS spectrometer, L IBS spectrometer transmits L IBS spectrum data to main controller in real time, with continuous sample injection, main controller continuously collects and stores dynamic liquid core wave L IBS spectrum data;
4) dynamic liquid core waveguide raman testing
While the dynamic liquid core waveguide L IBS test is carried out, the Raman laser emits a certain wavelength lambda from right to left1The continuous laser beam is expanded by a Raman beam expander and then passes through an interference filter to obtain a narrow-frequency Raman laser beam, then the narrow-frequency Raman laser beam passes through a Raman dichroic mirror, passes through a Raman microscope objective and passes through a window glass, and is focused into a liquid core waveguide tube to test a liquid sample, namely the urine of an astronaut, a Raman forward scattering signal excited at a focusing point is transmitted leftwards along the axis of the liquid core and continuously collides with molecules in the test liquid sample to obtain accumulation and reinforcement, the Raman forward scattering signal is transmitted to a total reflection mirror, is transmitted rightwards along the axis of the liquid core through reflection, and continuously collides with the molecules in the test liquid sample again, the Raman scattering signal is further reinforced, sequentially passes through the window glass and the Raman microscope, is reflected by the Raman dichroic mirror, is transmitted along the Raman receiving optical axis, and is filtered by the filter to remove the wavelength of the Rayle1The Raman scattering light after the pumping light enters the Raman receiving optical fiber through the focusing of the Raman fiber coupling mirror and is transmitted into the Raman spectrometer, the Raman spectrometer transmits Raman spectrum data to the main controller in real time, and the main controller continuously collects and stores the dynamic liquid core waveguide Raman spectrum data along with the continuous sampling;
5) real-time collection of waste liquid
In the testing process, sample introduction is continuously carried out; the test liquid sample in the liquid core waveguide tube is sprayed to the electric cathode along the spraying axis through the outflow tube, so that the test liquid sample with negative charges is attracted to the electric anode under the action of an electric field and enters the waste liquid tank;
6) test end and data post-processing
When the electric piston moves to the bottom of the right side in the liquid inlet device, the whole liquid inlet device is free of a test liquid sample, the test is finished, the main controller sends an instruction to close the delay controller, L IBS laser and L IBS spectrometer, then the main controller sends an instruction to close the Raman laser and the Raman spectrometer, the main controller carries out statistical accumulation processing on the obtained multiple groups of dynamic liquid core waveguide L IBS spectrum data to obtain L IBS signal detection of copper and sodium trace metals in urine reflecting the health condition of a astronaut, the main controller carries out statistical accumulation and average processing on the obtained multiple groups of dynamic liquid core waveguide Raman spectrum data to obtain Raman spectrum signals reflecting the health condition of the astronaut and related to the contents of creatinine, protein and urea in the urine, the signal data is compared with the health index, and the L IBS spectrum analysis result is fused with the Raman spectrum analysis result, so that the real-time diagnosis of part of the physiological indexes of the astronaut is realized;
7) post-treatment of residual waste liquid
The main controller sends a control command to the stepper motor to drive the electric piston to slowly translate leftwards in the liquid inlet device until reaching the leftmost position of the liquid inlet device; opening the sealing cover, introducing clean water into the liquid inlet device through the liquid inlet, and covering the sealing cover after the introduction is finished; the main controller sends a control instruction to the stepper motor to drive the electric piston to slowly translate to the right in the liquid inlet device, clean water is slowly pushed into the liquid core waveguide tube through the connecting hose and the inflow pipe, meanwhile, residual waste liquid in the liquid core waveguide tube is pressed into the collecting subsystem to be collected until the electric piston translates to the bottom of the right side in the liquid inlet device, at the moment, only clean water exists in the liquid core waveguide tube, the main controller sends an instruction, and the direct-current power supply is disconnected.
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