CN109355229A - A kind of lactobacillus plantarum and its application in the preparation of Chinese silvergrass ensilage - Google Patents
A kind of lactobacillus plantarum and its application in the preparation of Chinese silvergrass ensilage Download PDFInfo
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- CN109355229A CN109355229A CN201811397349.8A CN201811397349A CN109355229A CN 109355229 A CN109355229 A CN 109355229A CN 201811397349 A CN201811397349 A CN 201811397349A CN 109355229 A CN109355229 A CN 109355229A
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- chinese silvergrass
- lactobacillus plantarum
- feed
- lactobacillus
- ensilage
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- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 24
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 24
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 241000186660 Lactobacillus Species 0.000 claims abstract description 15
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 15
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- 238000009629 microbiological culture Methods 0.000 claims abstract description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims abstract description 3
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- 239000000835 fiber Substances 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
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- 230000015556 catabolic process Effects 0.000 abstract description 3
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- 230000001580 bacterial effect Effects 0.000 description 10
- 239000003795 chemical substances by application Substances 0.000 description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
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- 241000293268 Astragalus chinensis Species 0.000 description 1
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- 108700029181 Bacteria lipase activator Proteins 0.000 description 1
- 241000743756 Bromus inermis Species 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 241000209082 Lolium Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
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- 230000000996 additive effect Effects 0.000 description 1
- 239000004178 amaranth Substances 0.000 description 1
- 235000012735 amaranth Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
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- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
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- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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Abstract
The invention discloses a kind of lactobacillus plantarum and its applications in the preparation of Chinese silvergrass ensilage, belong to microorganism field, the lactobacillus plantarum EC018 is to be obtained by common plant lactobacillus by heavy ion mutagenesis, China General Microbiological culture presevation administrative center is deposited on October 8th, 2018, deposit number is CGMCC NO.:16556；The lactobacillus plantarum EC018 is mixed with thalline quantity ratio 1:1 with cheese type lactobacillus and is sprayed on Chinese silvergrass section surface, it can reduce the pH value of ensiling material, improve the content of soluble-carbohydrate and protein in Chinese silvergrass feed after fermenting, reduce ammonia nitrogen content, prevent the degradation of protein in feed, the content for reducing neutral detergent fiber and acid detergent fiber in feed improves the nutritional quality of feed, increases the feeding value of feed；Organic acid content in feed is increased simultaneously, improves the palatability of feed, becomes Chinese silvergrass using ensilage.
Description
Technical field
The invention belongs to microorganism fields, more particularly to a kind of lactobacillus plantarum and its in the preparation of Chinese silvergrass ensilage
Application.
Background technique
Chinese silvergrass is a kind of perennial C4 plant, with the speed of growth is fast, photosynthetic efficiency is high, biological yield is high, liquid manure demand
The advantages that low, pest and disease damage lacks is to be suitble to the fiber-like of marginal land plantation that can raise dual-purpose grass.Nan Di is the distinctive Chinese silvergrass product in China
Kind, the not only fine quality all with Chinese silvergrass, but also its tender cauline leaf nutritive value with higher contains higher concentration
Water soluble carbohydrates and crude protein, total effectively nutrient composition content is up to 55%, and being very suitable to exploitation is ensilage.
Current China's high quality grass scarcity of resources, it has also become restrict the bottleneck of animal husbandry development, for the development for accelerating animal husbandry, exploitation and
Expand feed resource to have become a top priority.High-quality to raise dual-purpose type Chinese silvergrass and get the attention, high-quality Chinese silvergrass ensiling is raised
Material is developed into important research topic.
Ensiling is to reduce pH value by making raw material generate organic acid using lactic acid bacteria anaerobic fermentation in ensiling material, inhibit
It is multiplied with various harmful microbes are killed, saves a kind of storage method that feed uses for a long time to reach.Ensiling biology
Feed is the important feed resource of ruminant, and ensilage can effectively save the nutritional ingredient of silage plant, guarantees that raw material is green
Fresh and tender juice when green, soft, the advantages that palatability is good, and digestibility is high, be the important of ruminant (such as ox, sheep)
Feed resource.Nutritive loss rate is 8%-10% after ensilage ensiling, and the nutritive loss rate for drying forage is 30%-
50%, it is even higher.Ensilage is in ensilage, and since the beneficial microorganisms such as lactic acid bacteria act on, the fiber of feed contains
Amount can decline 3%-6%, and crude protein content improves 0.5%-2%.Currently, feed often with ensiling raw material it is gramineous have corn,
Rye grass, awnless brome；Pulse family has clover, clover, Chinese milk vetch；Other root, stem and leaf classes have sweet potato, pumpkin, three-coloured amaranth, aquatic
Plant etc., and the ensiling of Chinese silvergrass is rarely reported, in presently disclosed patent document, China Patent No. ZL201110188280.X
" microbial bacterial agent and its preparation method and application for Chinese silvergrass class ensilage " discloses a kind of for Chinese silvergrass class ensiling feeding
Microbial bacterial agent of material and its preparation method and application.The microbial bacterial agent is used for the production of Chinese silvergrass class ensilage, shortens
The Chinese silvergrass ensilage fermentation time, protein content is increased, content of cellulose is reduced.
Summary of the invention
The technical problem to be solved in the present invention is that providing a kind of lactobacillus plantarum and its in the preparation of Chinese silvergrass ensilage
Application, solve the problems such as Chinese silvergrass ensiling in the prior art is difficult, and digestibility is low, and aquaculture cost is high.
The present invention is achieved by the following technical solution:
The preparation method of the lactobacillus plantarum EC018: a kind of lactobacillus plantarum EC018 uses heavy ion radiation mutagenesis
Method carries out heavy ion radiation mutagenesis to common plant lactobacillus, and mutagenesis energy heavy ion beam current is12C6+, energy 80MeV/
U, dose of radiation 50GY, radiated time 5min, the lactobacillus plantarum after irradiation obtain after the screening of plate streaking culture repeatedly,
China General Microbiological culture presevation administrative center is deposited on October 8th, 2018, deposit number is CGMCC NO.:16556,
Preservation address Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；
Application of the lactobacillus plantarum EC018 in the preparation of Chinese silvergrass ensilage, includes the following steps:
1) the in due course harvest of Chinese silvergrass；
In Chinese silvergrass vegetative growth phase (8-9 leaf phase), Chinese silvergrass harvest is carried out, height for the stubble left 3-5cm sets Chinese silvergrass after harvesting
It is dried 3-4 hours under sunlight, shines to water content and carry out next step operation in 65%-75%；
2) Chinese silvergrass hand hay cutter section is rubbed into silk: by the hay cutter cutting 2-3cm segment of Chinese silvergrass after sunning；
3) lactobacillus mix preparation is uniformly sprayed；
Using spraying or spray pattern, lactobacillus plantarum EC018 is mixed with cheese type lactobacillus with thalline quantity ratio 1:1
It closes, concentration 109Cfu/mL, the Chinese silvergrass section surface after being uniformly sprayed on step 2) cutting, per kilogram Chinese silvergrass spray 100mL；
4) ensiling is vacuum-packed；
Using vacuum packing machine, sealing is stored in 25 DEG C of room temperature environments being protected from light after Chinese silvergrass material is vacuumized.Ensiling
Time is 60 days.
The present invention compared with prior art the utility model has the advantages that
The method of the present invention can effectively improve the organoleptic quality after Chinese silvergrass fermentation, make the condition and appearance of Chinese silvergrass feed after ensiling
Be greatly improved, the present invention utilize mutagenesis after obtain lactobacillus plantarum EC018 mixed with cheese type lactobacillus after to Chinese silvergrass into
Row ensiling can not only reduce the pH value of ensiling material, corrode fermented material from other harmful miscellaneous bacterias, effective protection blueness
Store feed；And the content of soluble-carbohydrate and protein in Chinese silvergrass feed after fermentation can also be improved, reduce ammoniacal nitrogen
Content effectively prevents the degradation of protein in feed, protects the loss of nutriment, and it is fine to reduce neutral detergent in feed
The content of peacekeeping acid detergent fiber improves the nutritional quality of feed, increases the feeding value of feed；Feeding is increased simultaneously
The content of organic acid content in material, especially lactic acid and acetic acid improves the palatability of feed, and the mouthfeel and nutrition for improving feed are inhaled
Value is received, becomes Chinese silvergrass using ensilage.
Specific embodiment
Technical solution of the present invention is further explained below by embodiment, but protection scope of the present invention not by
The limitation of embodiment in any form.
Embodiment 1,
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
The Chinese silvergrass kind for ensilage preparation in following embodiments is southern reed, can be obtained from variety right people, only
It can be used for repeating present invention experiment to use.
(1) the quick preparation of lactobacillus mix bacterium agent
1) MRS culture medium (1L)
Liquid: peptone 10g, yeast extract 5g, K2HPO42g, diammonium hydrogen citrate 2g, sodium acetate 5g, glucose 20g,
Beef extract 10g, MgSO4·7H2O 0.58g、MnSO4·H2O 0.25g, Tween 80 1ml；
Solid: agar 15gCaCO3 10g
Preparation method method: being added triangular flask for mentioned component, adds water 1L, and the stirring and dissolving on magnetic stirring apparatus, is made
Fluid nutrient medium；To prepare solid medium, also needs that 1.5% agar is added, stir to being completely dissolved, in addition by 1%
Calcium carbonate is wrapped with newspaper, individually placed, and sterilize 15-20min at 121 DEG C.
2) preparation method of the lactobacillus plantarum EC018 is
Using heavy ion radiation method of mutagenesis, radioinduction, mutagenesis energy heavy ion beam are carried out to common plant lactobacillus
Stream is12C6+, energy 80MeV/u, dose of radiation 50GY, radiated time 5min, through the sieve of plate streaking culture repeatedly after irradiation
Choosing gained, is deposited in China General Microbiological culture presevation administrative center (China General on October 8th, 2018
Microbiological Culture Collection Center, CGMCC), deposit number: CGMCC NO.:16556, preservation
Address Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
3) activation and preservation of bacterial strain
Take the bacterial strain of -20 DEG C of preservations after MRS solid medium crosses culture 16h, picking single bacterium falls within MRS Liquid Culture
16h is cultivated at 37 DEG C of base；The bacterial strain newly accessed is taken in 300ul to 1.5mlEP pipe again, the sterile glycerol of 700ul is added, is mixed
Afterwards in -20 DEG C of preservations.
Activated strains are accessed MRS fluid nutrient medium by 10% inoculum concentration by volume, and culture surveys OD value afterwards for 24 hours, works as bacterium colony
Concentration reaches 109Cfu/ml, so that it may to carry out being used for Chinese silvergrass ensiling after two kinds of bacterium solutions mix.
(2) Chinese silvergrass harvests in due course
In Chinese silvergrass vegetative growth phase, Chinese silvergrass harvest is carried out, the place ground proximity 3-5cm partition harvesting when harvest, general after harvesting
Chinese silvergrass, which is placed under sunlight, dries 3-4 hours (general morning harvesting sunning, carry out ensiling afternoon), shines to moisture in 65%-75%
Carry out next step operation.
Cutting rubs silk for Chinese silvergrass hay cutter cutting 2-3cm segment after sunning after Chinese silvergrass harvest.
Add microbial bacterial agent: the added microbial bacterial agent of the embodiment is traditional plant lactobacillus and cheese type lactobacillus
Mixed liquor, concentration 109Cfu/ml is defined as CK group, is uniformly sprayed on ensiling material surface using spraying or spray pattern, often
Kilogram Chinese silvergrass sprays 100ml.
Vacuumize storage: using vacuum packing machine, sealing is stored in 25 DEG C of room temperatures being protected from light after ensiling material is vacuumized
In environment, period of storage is 60 days or more.
Silage Quality measurement: after ferment, feed being mixed, and takes appropriate progress sample analysis identification.
Embodiment 2
The added microbial bacterial agent of the embodiment is lactobacillus plantarum EC018 and cheese type lactobacillus mixed liquor, and concentration is
109Cfu/mL is defined as MIX group, remaining Silaging method is same as Example 1.
The ensilage of each embodiment prepared using Chinese silvergrass as raw material is evaluated:
1. sensory evaluation
1 ensiling material sensory evaluation of table
As seen from the above table, microbial bacterial agent provided by the invention, the sensory profiles after capable of effectively improving Chinese silvergrass fermentation, makes blueness
The condition of Chinese silvergrass feed, appearance are all greatly improved after storage.
2. Silage Quality compares
Chinese silvergrass Silage Quality compares under 2 different microorganisms additive of table
As can be seen from the above table, the present invention is mixed using acquisition lactobacillus plantarum EC018 after mutagenesis with cheese type lactobacillus
Ensiling is carried out to Chinese silvergrass afterwards, can not only reduce the pH value of ensiling material, fermented material is corroded from other harmful miscellaneous bacterias, has
Effect protects ensilage；And the content of soluble-carbohydrate and protein in Chinese silvergrass feed after fermentation can also be improved,
Ammonia nitrogen content is reduced, the degradation of protein in feed is effectively prevented, protects the loss of nutriment, reduce in feed
The content of neutral detergent fiber and acid detergent fiber improves the nutritional quality of feed, increases the feeding value of feed；Together
When increase the content of organic acid content in feed, especially lactic acid, improve the palatability of feed, improve mouthfeel and the battalion of feed
It supports and absorbs value.Therefore, the Silage Quality of Chinese silvergrass feed can be further increased by the method for the invention, and production palatability is good
Livestock feed.
Claims (3)
1. a kind of lactobacillus plantarum EC018, it is characterised in that the lactobacillus plantarum EC018 was deposited on October 8th, 2018
China General Microbiological culture presevation administrative center, deposit number are CGMCC NO.:16556.
2. a kind of lactobacillus plantarum EC018 according to claim 1, it is characterised in that the lactobacillus plantarum EC018's
Preparation method: using heavy ion radiation method of mutagenesis, carries out heavy ion radiation mutagenesis, mutagenesis energy weight to common plant lactobacillus
Ion beam current is12C6+, energy 80MeV/u, dose of radiation 50GY, radiated time 5min, the lactobacillus plantarum warp after irradiation
It is obtained after plate streaking culture screening repeatedly.
3. a kind of lactobacillus plantarum EC018 according to claim 1, it is characterised in that the lactobacillus plantarum EC018 exists
Application in the preparation of Chinese silvergrass ensilage, includes the following steps:
1) the in due course harvest of Chinese silvergrass；
In Chinese silvergrass vegetative growth phase, Chinese silvergrass harvest is carried out, Chinese silvergrass is placed under sunlight after harvesting and dries by height for the stubble left 3-5cm
It 3-4 hours, shines to water content and carries out next step operation when mass ratio is 65%-75%；
2) Chinese silvergrass hand hay cutter section is rubbed into silk: by the hay cutter cutting 2-3cm segment of Chinese silvergrass after sunning；
3) lactobacillus mix preparation is uniformly sprayed；
Using spraying or spray pattern, lactobacillus plantarum EC018 is mixed with cheese type lactobacillus with thalline quantity ratio 1:1, it is dense
Degree is 109Cfu/mL, the Chinese silvergrass section surface after being uniformly sprayed on step 2) cutting, per kilogram Chinese silvergrass spray 100mL；
4) ensiling is vacuum-packed；
Using vacuum packing machine, sealing is stored in 25 DEG C of room temperature environments being protected from light after Chinese silvergrass material is vacuumized, the ensiling time
It is 60 days.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102250807A (en) * | 2011-07-06 | 2011-11-23 | 广西工学院 | Microbial agent for Chinese silvergrass ensilage as well as preparation method and application thereof |
| CN108060104A (en) * | 2018-01-29 | 2018-05-22 | 四川农业大学 | A kind of lactobacillus plantarum and its application, screening calibration method |
| CN108102975A (en) * | 2018-02-05 | 2018-06-01 | 山东江信动物保健科技有限公司 | One plant for the lactobacillus plantarum for the green forage that ferments and its production method of microbial inoculum |
| CN108570421A (en) * | 2017-03-09 | 2018-09-25 | 北京大北农科技集团股份有限公司 | One lactobacillus plantarum strain and its ensilage fermentation agent |
| CN108795800A (en) * | 2018-05-25 | 2018-11-13 | 兰州大学 | The lactobacillus plantarum of one plant of production feruloyl esterase |
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| EP1438901A1 (en) * | 2003-01-14 | 2004-07-21 | Ajinomoto Co., Inc. | Silage additive and a process for preparing silage using it |
| CN102864095B (en) * | 2012-04-18 | 2014-08-20 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum used for silage alfalfa and use method thereof |
| CN102864093B (en) * | 2012-04-18 | 2014-01-15 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum suitable for silage straw and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250807A (en) * | 2011-07-06 | 2011-11-23 | 广西工学院 | Microbial agent for Chinese silvergrass ensilage as well as preparation method and application thereof |
| CN108570421A (en) * | 2017-03-09 | 2018-09-25 | 北京大北农科技集团股份有限公司 | One lactobacillus plantarum strain and its ensilage fermentation agent |
| CN108060104A (en) * | 2018-01-29 | 2018-05-22 | 四川农业大学 | A kind of lactobacillus plantarum and its application, screening calibration method |
| CN108102975A (en) * | 2018-02-05 | 2018-06-01 | 山东江信动物保健科技有限公司 | One plant for the lactobacillus plantarum for the green forage that ferments and its production method of microbial inoculum |
| CN108795800A (en) * | 2018-05-25 | 2018-11-13 | 兰州大学 | The lactobacillus plantarum of one plant of production feruloyl esterase |
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