CN1093409A - Coliform quick test test paper and production method thereof - Google Patents
Coliform quick test test paper and production method thereof Download PDFInfo
- Publication number
- CN1093409A CN1093409A CN 93110616 CN93110616A CN1093409A CN 1093409 A CN1093409 A CN 1093409A CN 93110616 CN93110616 CN 93110616 CN 93110616 A CN93110616 A CN 93110616A CN 1093409 A CN1093409 A CN 1093409A
- Authority
- CN
- China
- Prior art keywords
- paper
- solution
- weight
- test
- coliform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of microbial testing technology, particularly a kind of coliform quick test is with test paper and production method, it is by the miscible back of lactose, cholate, peptone, sodium laurylsulfonate, dipotassium hydrogen phosphate, 1.6% purpurum bromocresolis solution, 4% TCC solution and water generate a reagent, after low pound sterilization, filter paper is immersed and soaks into, pull drying, pack out.The present invention compared with prior art has characteristics such as production technique is simple, cost is low, go out the result time is fast and accurate, can increase work efficiency 2～3 times, alleviates reviewer's labour intensity greatly.
Description
The present invention relates to a kind of microbial testing technology, particularly a kind of coliform quick test test paper and production method thereof.
Coliform is an important indicator of check diet product, water and external environment pollution level.At present, what China continued to use is the fermentation method that is undertaken by the State Standard of the People's Republic of China, and this method needed 72 hours just can go out the result, and the check formality is miscellaneous, bring many inconvenience, particularly cold drinks corporation to the reviewer, will build one at least and deposit the freezer of producing cold drink more than three days.
For reaching the purpose of easy quick test coliform, the quick scraps of paper of a kind of coliform have appearred on the market, with this scraps of paper check coliform, fast and convenient than fermentation method, but production cost is higher, cause valuable product, general foodstuff production producer is difficult to accept, and go out time of result also will be after 15 hours.
The objective of the invention is to overcome above the deficiencies in the prior art part and provide that a kind of production technique is simple, cost is low, the method for inspection is easy, the coliform quick test test paper of processing ease.
Above-mentioned purpose of the present invention reaches in the following way: the coliform quick test comprises the packing bag and the scraps of paper with test paper, and the scraps of paper are lactose, the cholate of 0.15-0.6%, the peptone of 1-4%, the sodium laurylsulfonate of 0.2-1.0%, the dipotassium hydrogen phosphate of 0.2-0.7%, the 0.3-0.6%(weight that is soaked with 0.7-3%) 1.6% purpurum bromocresolis solution and 1.8-3.0%(weight) 4% TCC solution miscible in 87.1-95.7%(weight) water in the filter paper of the reagent made.
Another object of the present invention is to overcome the deficiencies in the prior art and a kind of production technique is simple, cost the is low coliform quick test production method with test paper is provided.
Above-mentioned purpose of the present invention is achieved by the following technical solution: the lactose of getting 0.7-3%, the cholate of 0.15-0.6%, the peptone of 1-4%, the sodium laurylsulfonate of 0.2-1.0%, the dipotassium hydrogen phosphate of 0.2-0.7%, 0.3-0.6%(weight) 1.6% purpurum bromocresolis solution and 1.8-3.0%(weight) 4% TCC solution miscible in 87.1-95.7%(weight) water in generate a reagent, then, with mentioned reagent sterilization after 15 minutes under low pound condition, pour in the aseptic container, filter paper is immersed, pull out after treating to soak into fully, under 37 ° of-40 ℃ of temperature, dry, cut the back aseptic technique by specification and pack into to seal in the disinfectant packing bag and form.
The present invention is owing to adopt as above design, compared with prior art, have that production technique is simple, cost is low, go out fast advantages such as (can in 12-14 hour, go out the result) of time as a result, the 2-3 that can increase work efficiency doubly, and go out result's accuracy rate height, need not cleaning glass apparatus during use, thereby alleviated reviewer's labour intensity greatly.
Below in conjunction with drawings and Examples detailed structure of the present invention is described further.
Accompanying drawing 1 is a structural representation of the present invention.
Embodiment:
Get lactose 10.0g, cholate 3.0g, peptone 20.0g, sodium laurylsulfonate 2.0g, dipotassium hydrogen phosphate 3.5g, 1.6% purpurum bromocresolis solution 5.0ml, 4% TCC solution 20ml miscible in 1000ml water generate a reagent, after the sterilization in 15 minutes of low pound, reagent is poured in the aseptic big porcelain basin, with 100 10 * 60(cm
2) filter paper immerse, after treating to soak into fully, pull out under 37 °～40 ℃ temperature and dry, be cut into the scraps of paper (1) by required specification, in disinfectant plastics bag (2) is packed in aseptic technique into then, put into refrigerator after the packing and preserve standby.
Its using method is as follows:
One, gets the liquid sample of coliform＜3; Specification is 10 * 10(cm)
2The scraps of paper three sacks, every bag of application of sample 10ml; Specification is 5 * 5(cm)
2The scraps of paper six pouches, add stoste and 1: 10 diluent 1.0ml respectively, each three pouch of two kinds of concentration.
Two, sample is inoculated in the scraps of paper after, under 35 ° of-37 ℃ of temperature, cultivated 12-14 hour, observations is according to positive scraps of paper number, computation of table lookup MPN number.
Three, raw paper sheet is green, and the scraps of paper yellow expression ferment lactose occurs and produce acid after cultivating, red point occurs and represent gram negative bacillus.The scraps of paper of coliform-positive be the centre have red point, on every side for the xanchromatic scraps of paper.
Claims (4)
1, a kind of coliform quick test test paper, it is characterized in that comprising the packing bag and the scraps of paper, the scraps of paper are the filter paper of the miscible reagent of making in 87.1-95.7% (weight) water of 4% TCC solution of the 1.6% purpurum bromocresolis solution of dipotassium hydrogen phosphate, 0.3-0.6% (weight) of peptone, the 0.2-0.7% of the cholate that is soaked with lactose with 0.7-3%, 0.15-0.6%, 1-4% and 1.3-3.0% (weight).
2, test paper as claimed in claim 1 is characterized in that the described scraps of paper are the filter paper that is soaked with 10.0g lactose, 3.0g cholate, 200g peptone, 3.5g dipotassium hydrogen phosphate, 2.0g sodium laurylsulfonate, the purple solution of 5.0ml1.6% bromine formic acid and the miscible reagent of making of 20.0ml4% TCC solution in 1000ml water.
3, a kind of coliform quick test production method of test paper, it is characterized in that getting the lactose of 0.7-3%, the cholate of 0.15-0.6%, the peptone of 1-4%, the sodium laurylsulfonate of 0.2-1.0%, the dipotassium hydrogen phosphate of 0.2-0.7%, 0.3-0.6%(weight) 1.6% purpurum bromocresolis solution and 1.8-3.0%(weight) 4% TCC solution be dissolved in 87.1～95.7%(weight) water in generate a reagent, mentioned reagent sterilization under low pound condition was poured in the aseptic container after 15 minutes, filter paper is immersed, pull out after treating to soak into fully, under 37 ℃～40 ℃ temperature, dry, cut the back aseptic technique by specification and pack into and seal in the disinfectant packing bag.
4, production method as claimed in claim 3 is characterized in that described reagent makes in 1000ml water by 10.0g lactose, 3.0g cholate, 20.0g peptone, 2.0g sodium laurylsulfonate, 3.5g dipotassium hydrogen phosphate, 5.0ml1.6% purpurum bromocresolis solution and 20.0ml4% TCC solution are miscible.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93110616 CN1093409A (en) | 1993-03-06 | 1993-03-06 | Coliform quick test test paper and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93110616 CN1093409A (en) | 1993-03-06 | 1993-03-06 | Coliform quick test test paper and production method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1093409A true CN1093409A (en) | 1994-10-12 |
Family
ID=4988464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93110616 Pending CN1093409A (en) | 1993-03-06 | 1993-03-06 | Coliform quick test test paper and production method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1093409A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113103C (en) * | 1996-02-14 | 2003-07-02 | 马永健 | CO2 absorption indicator and reagent kit for diagnosing pyloric Helicobacterium |
| CN101294190B (en) * | 2007-12-05 | 2011-05-04 | 姚毓才 | Composite quick colour-developing examination and check agent for coliform group bacteria, researching and developing flow process thereof |
| CN105999366A (en) * | 2016-07-28 | 2016-10-12 | 广东泰宝医疗科技股份有限公司 | Preparation method of color-developing medical dressing |
-
1993
- 1993-03-06 CN CN 93110616 patent/CN1093409A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113103C (en) * | 1996-02-14 | 2003-07-02 | 马永健 | CO2 absorption indicator and reagent kit for diagnosing pyloric Helicobacterium |
| CN101294190B (en) * | 2007-12-05 | 2011-05-04 | 姚毓才 | Composite quick colour-developing examination and check agent for coliform group bacteria, researching and developing flow process thereof |
| CN105999366A (en) * | 2016-07-28 | 2016-10-12 | 广东泰宝医疗科技股份有限公司 | Preparation method of color-developing medical dressing |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Characklis | Attached microbial growths—I. Attachment and growth | |
| JP3117998B2 (en) | Apparatus and method for growing anaerobic microorganisms | |
| US5482860A (en) | Apparatus for continuously removing oxygen from fluid streams | |
| CN108130256A (en) | A kind of multifunctional rice wine fermentation tank and its process | |
| CN201850276U (en) | Micro-filtration membrane coupling high-density culture device for lactic acid bacteria | |
| Reed et al. | Baker’s yeast production | |
| CN107022541B (en) | Aspergillus niger immobilization method | |
| CN107488600A (en) | One plant height produces the aspergillus niger of resistance to oxidation low temperature glucose oxidase | |
| CN104673876B (en) | A kind of transparent water absorbent gel and detection plate for microorganism detection | |
| CN1093409A (en) | Coliform quick test test paper and production method thereof | |
| CN112724474B (en) | Cold hydrogel and application thereof | |
| US2422777A (en) | Method for producing penicillin by mold culture | |
| CN114561328A (en) | Preparation method of high-activity lactobacillus agent | |
| CN111057652A (en) | Preparation process of microbial agent based on directional screening of microorganisms | |
| VAUGHN et al. | Gram negative bacteria associated with sloughing, a softening of California ripe olives | |
| CN104498415A (en) | Complex microbial inoculant rich in grease raw material for medium-temperature biogas fermentation system and preparation method and application thereof | |
| Bushnell | Quantitative determinations of some of the biochemical changes produced by a saprophytic anaerobe | |
| CN201142921Y (en) | Installation for fermenting pickling vegetable | |
| CN213490886U (en) | Totally closed sterilization jar device is used in microbial fermentation | |
| CN110777187A (en) | Microorganism type time-temperature indicator | |
| Brockett | Some aspects of the microbiological activity of the Mangere oxidation ponds | |
| CN212102756U (en) | Breathe and purify fermentation cylinder structure | |
| Updegraff | The production of phenol and para-cresol by marine bacteria | |
| Thompson | The group of hydrogen sulphide producing bacteria | |
| CN102086084B (en) | Method for preparing heavy metal finishing agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |