CN111057652A - Preparation process of microbial agent based on directional screening of microorganisms - Google Patents
Preparation process of microbial agent based on directional screening of microorganisms Download PDFInfo
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- CN111057652A CN111057652A CN202010157788.2A CN202010157788A CN111057652A CN 111057652 A CN111057652 A CN 111057652A CN 202010157788 A CN202010157788 A CN 202010157788A CN 111057652 A CN111057652 A CN 111057652A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Abstract
The invention discloses a preparation process of a microbial agent based on directional screening of microorganisms. The invention completes the preparation process of the microbial agent, has high production efficiency, can repeatedly use waste residues as a compound fertilizer, prevents waste materials from being wasted, has high yield of the obtained microbial agent, and is suitable for large-scale popularization and use.
Description
Technical Field
The invention belongs to the technical field of fermentation, and particularly relates to a preparation process of a microbial agent based on directional screening of microorganisms.
Background
The microbial agent is a low-carbon, purely natural, harmless and pollution-free biological raw material, belongs to the green industry which changes waste into valuable, improves the added value of the bacterial residues, increases the production benefit of production enterprises by utilizing the advanced technology of technological innovation, and further enhances the sustainable development of pharmaceutical enterprises.
At present, domestic agricultural products are low in price, domestic agricultural raw materials are insufficient in production and supply, and the microbial inoculum has wide application prospects in agricultural directions such as organic fertilizers, feeds and the like. At present, domestic antibiotic fungi residue dry powder is more than 200 million tons per year, and the secondary utilization efficiency is low, so that resource waste and secondary environmental pollution are caused.
The prior art has low production efficiency, low yield, more generated waste residues and is not practical, so a new preparation process is needed to improve the efficiency.
Disclosure of Invention
The object of the present invention is to provide: a preparation process of a microbial agent based on the directional screening of microorganisms, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a process for preparing the microbial bacterial preparation based on the directional screening of microbes includes such steps as providing the microbes to be used, purifying, separating to obtain pure bacterial strains, screening by liquid and solid screening formulas, fermenting, and separating to obtain said microbial bacterial preparation.
Further, the method comprises the following steps:
s1: firstly, preparing a seeding tank, and sterilizing the seeding tank for 0.5-1h by using steam;
s2: after sterilization, putting a culture medium required by the microbial agent into a seeding tank, and testing the pH value of the culture medium in the seeding tank until the pH value reaches 6.5-7.5;
s3: after the PH debugging is finished, sterilizing the seed tank for 0.5-1h by using steam again, and then cooling the seed tank by inputting sterilized air;
s4: after the temperature is reduced, carrying out inoculation treatment on the seed tank, adding microbial strains, and then adding sterile air and a defoaming agent to ferment the microbial strains in the seed tank;
s5: after the microbial bacteria in the fermentation tank are fermented, putting the mixture into a storage tank, and then carrying out solid-liquid separation to obtain a liquid compound fertilizer and moist solid microbial bacteria;
s6: and pouring the moist solid microbial bacteria into an adsorption stirring tank, conveying the solid microbial bacteria to a dryer by a spiral dragon conveyor for drying, and separating to obtain the powdery microbial bacteria agent and the compound fertilizer.
Further, the steam temperature in the step S1 and the step S3 is 121-125 ℃, and the pressure is 0.103-0.168 MPa.
Further, the volume of the culture medium added in the step S2 is 50% -75% of the volume of the seeding tank.
Further, the means for measuring the PH of the seed tank in step S2 is a precision PH paper or a PH meter.
Further, the temperature in the step S3 is 25-35 ℃ and is under normal pressure; in the step S3, the temperature is 25-35 ℃, and the mixture needs to be placed for 24-46 h.
Furthermore, in step S3, the inside of the seed tank needs to be inspected by a microscope, and the morphology, density and spore formation rate of the cells need to be inspected, and the spore formation rate is ≧ 80%.
Further, in the step S5, the storage tank is at normal temperature and normal pressure, and the solid content of the liquid microbial inoculum is 10% -20%.
Further, CaCO is required to be added in the step S63Or humic acid 80 meshes is used as an adsorption medium, the feeding amount of the adsorption medium is 1-3:1, and the water content after adsorption is 25% -50%.
Further, the temperature of the drying heat medium in the step S6 is 100-120 ℃, the moisture of the dried microbial agent is less than 10%, and the content of the obtained microbial agent is 25-200 hundred million/g under normal pressure.
Compared with the prior art, the invention has the beneficial effects that:
the preparation process of the microbial agent is completed through the steps S1-S6, the production efficiency is high, the waste residue can be repeatedly used as a compound fertilizer, waste materials cannot be wasted, the yield of the obtained microbial agent is high, and the preparation process is suitable for being popularized and used in large quantities.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
In order to facilitate an understanding of the present invention, a more complete description of the present invention is provided below. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The invention provides a technical scheme that:
a process for preparing the microbial bacterial preparation based on the directional screening of microbes includes such steps as providing the microbes to be used, purifying, separating to obtain pure bacterial strains, screening by liquid and solid screening formulas, fermenting, and separating to obtain said microbial bacterial preparation.
In order to further improve the use function of the preparation process of the microbial agent based on the directional screening of the microorganisms, the preparation method comprises the following steps:
s1: firstly, preparing a seeding tank, and sterilizing the seeding tank for 0.5-1h by using steam;
s2: after sterilization, putting a culture medium required by the microbial agent into a seeding tank, and testing the pH value of the culture medium in the seeding tank until the pH value reaches 6.5-7.5;
s3: after the PH debugging is finished, sterilizing the seed tank for 0.5-1h by using steam again, and then cooling the seed tank by inputting sterilized air;
s4: after the temperature is reduced, carrying out inoculation treatment on the seed tank, adding microbial strains, and then adding sterile air and a defoaming agent to ferment the microbial strains in the seed tank;
s5: after the microbial bacteria in the fermentation tank are fermented, putting the mixture into a storage tank, and then carrying out solid-liquid separation to obtain a liquid compound fertilizer and moist solid microbial bacteria;
s6: and pouring the moist solid microbial bacteria into an adsorption stirring tank, conveying the solid microbial bacteria to a dryer by a spiral dragon conveyor for drying, and separating to obtain the powdery microbial bacteria agent and the compound fertilizer.
In order to further improve the use function of the preparation process of the microbial agent based on the directional screening of the microorganisms, the steam temperature in the step S1 and the step S3 is 121-125 ℃, and the pressure is 0.103-0.168 MPa.
In order to further improve the use function of the preparation process of the microbial agent based on the directional screening of the microorganisms, the volume of the culture medium added in the step S2 is 50-75% of the volume of the seeding tank.
In order to further improve the utilization of the process for preparing a microorganism inoculant based on the directional screening of microorganisms, the means for measuring the pH of the seeding tank in step S2 is a precision pH paper or a pH meter.
In order to further improve the use function of the preparation process of the microbial agent based on the directional screening of the microorganisms, the temperature in the step S3 is 25-35 ℃ and is normal pressure; in the step S3, the temperature is 25-35 ℃, and the mixture needs to be placed for 24-46 h.
In order to further improve the use function of the process for preparing a microbial agent by directed screening of microorganisms, the step S3 requires microscopic examination of the interior of the seed tank, and requires examination of the morphology, density and sporulation rate of the cells, and the sporulation rate is not less than 80%.
In order to further improve the use function of the preparation process of the microbial agent based on the directional screening of the microorganisms, the storage tank in the step S5 is at normal temperature and normal pressure, and the solid content of the liquid microbial agent is 10% -20%.
In order to further improve the use function of the preparation process of the microorganism bacterium agent based on the directional screening of the microorganism, the step S6 needs to add CaCO3Or humic acid 80 meshes is used as an adsorption medium, the feeding amount of the adsorption medium is 1-3:1, and the water content after adsorption is 25% -50%.
In order to further improve the use function of the preparation process of the microbial agent based on the directional screening of the microorganisms, the temperature of the drying heat medium in the step S6 is 100-120 ℃, the moisture of the dried microbial agent is less than 10%, and the content of the obtained microbial agent is 25-200 hundred million/g under normal pressure.
The working principle is as follows: firstly, preparing a seeding tank, and sterilizing the seeding tank for 0.5-1h by using steam; after sterilization, putting a culture medium required by the microbial agent into a seeding tank, and testing the pH value of the culture medium in the seeding tank until the pH value reaches 6.5-7.5; after the PH debugging is finished, sterilizing the seed tank for 0.5-1h by using steam again, and then cooling the seed tank by inputting sterilized air; after the temperature is reduced, carrying out inoculation treatment on the seed tank, adding microbial strains, and then adding sterile air and a defoaming agent to ferment the microbial strains in the seed tank; after the microbial bacteria in the fermentation tank are fermented, putting the mixture into a storage tank, and then carrying out solid-liquid separation to obtain a liquid compound fertilizer and moist solid microbial bacteria; and pouring the moist solid microbial bacteria into an adsorption stirring tank, conveying the solid microbial bacteria to a dryer by a spiral dragon conveyor for drying, and separating to obtain the powdery microbial bacteria agent and the compound fertilizer.
The preparation process of the microbial agent is completed through the steps S1-S6, the production efficiency is high, the waste residue can be repeatedly used as a compound fertilizer, waste materials cannot be wasted, the yield of the obtained microbial agent is high, and the preparation process is suitable for being popularized and used in large quantities.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A preparation process of a microbial agent based on directional screening of microorganisms is characterized by comprising the following steps: firstly, acquiring needed applicable microorganisms, purifying and separating to obtain pure strains, performing applicable screening on the pure strains through a liquid screening formula and a solid screening formula, then fermenting, and separating to obtain the microbial agent.
2. The process for preparing the microbial agent based on the directional screening of the microorganisms according to claim 1, which comprises the following steps:
s1: firstly, preparing a seeding tank, and sterilizing the seeding tank for 0.5-1h by using steam;
s2: after sterilization, putting a culture medium required by the microbial agent into a seeding tank, and testing the pH value of the culture medium in the seeding tank until the pH value reaches 6.5-7.5;
s3: after the PH debugging is finished, sterilizing the seed tank for 0.5-1h by using steam again, and then cooling the seed tank by inputting sterilized air;
s4: after the temperature is reduced, carrying out inoculation treatment on the seed tank, adding microbial strains, and then adding sterile air and a defoaming agent to ferment the microbial strains in the seed tank;
s5: after the microbial bacteria in the fermentation tank are fermented, putting the mixture into a storage tank, and then carrying out solid-liquid separation to obtain a liquid compound fertilizer and moist solid microbial bacteria;
s6: and pouring the moist solid microbial bacteria into an adsorption stirring tank, conveying the solid microbial bacteria to a dryer by a spiral dragon conveyor for drying, and separating to obtain the powdery microbial bacteria agent and the compound fertilizer.
3. The process for preparing a microbial inoculum based on the directional screening of microorganisms according to claim 2, which is characterized in that: the steam temperature in the step S1 and the step S3 is 121-125 ℃, and the pressure is 0.103-0.168 MPa.
4. The process for preparing a microbial inoculum based on the directional screening of microorganisms according to claim 2, which is characterized in that: the volume of the culture medium added in the step S2 is 50% -75% of the volume of the seeding tank.
5. The process for preparing a microbial inoculum based on the directional screening of microorganisms according to claim 2, which is characterized in that: the means for measuring the PH of the seed tank in step S2 is a precision PH paper or PH meter.
6. The process for preparing a microbial inoculum based on the directional screening of microorganisms according to claim 2, which is characterized in that: the temperature in the step S3 is 25-35 ℃ and is under normal pressure; in the step S3, the temperature is 25-35 ℃, and the mixture needs to be placed for 24-46 h.
7. The process for preparing a microbial inoculum based on the directional screening of microorganisms according to claim 2, which is characterized in that: in step S3, the inside of the seed tank needs to be subjected to microscopic examination, and the morphology, density and spore formation rate of the bacteria need to be checked, and the spore formation rate is ≧ 80%.
8. The process for preparing a microbial inoculum based on the directional screening of microorganisms according to claim 2, which is characterized in that: the storage tank in the step S5 is at normal temperature and normal pressure, and the solid content of the liquid microbial inoculum is 10-20%.
9. The process for preparing a microbial inoculum based on the directional screening of microorganisms according to claim 2, which is characterized in that: CaCO is required to be added in the step S63Or humic acid 80 meshes is used as an adsorption medium, the feeding amount of the adsorption medium is 1-3:1, and the water content after adsorption is 25% -50%.
10. The process for preparing a microbial inoculum based on the directional screening of microorganisms according to claim 2, which is characterized in that: the temperature of the drying heat medium in the step S6 is 100-120 ℃, the moisture of the dried microbial agent is less than 10%, and the content of the obtained microbial agent is 25-200 hundred million/g under normal pressure.
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| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
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| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
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| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
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| CN110684691A (en) * | 2019-10-23 | 2020-01-14 | 陕西麦可罗生物科技有限公司 | Preparation process of microbial agent based on directional screening of microorganisms |
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