CN109321632A - A kind of RNA cellular elements method of inspection - Google Patents
A kind of RNA cellular elements method of inspection Download PDFInfo
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- CN109321632A CN109321632A CN201811236925.0A CN201811236925A CN109321632A CN 109321632 A CN109321632 A CN 109321632A CN 201811236925 A CN201811236925 A CN 201811236925A CN 109321632 A CN109321632 A CN 109321632A
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- 238000000034 method Methods 0.000 title claims abstract description 33
- 230000001413 cellular effect Effects 0.000 title claims abstract description 31
- 238000007689 inspection Methods 0.000 title claims abstract description 14
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000009527 percussion Methods 0.000 claims abstract description 21
- 239000011521 glass Substances 0.000 claims abstract description 5
- 238000004043 dyeing Methods 0.000 claims abstract description 4
- 238000009434 installation Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 238000002156 mixing Methods 0.000 abstract description 6
- 230000006378 damage Effects 0.000 abstract description 5
- 238000000423 cell based assay Methods 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 abstract description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
- 238000013016 damping Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 241000726445 Viroids Species 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to cell detection fields, specifically a kind of RNA cellular elements method of inspection, this method includes mixing RNA cellular elements in glass container with pyrrole Luo Hong, then will carry out being sufficiently mixed dyeing in the cellular elements agitating device of the low sporoderm-broken rate of mixed liquor method；The cellular elements agitating device for the low sporoderm-broken rate that this method uses includes stocking mechanism, the inside of stocking mechanism is installed by percussion mechanism, percussion mechanism installs driving mechanism, driving mechanism drives percussion mechanism to move back and forth, so that percussion mechanism be made to drive the shaking of stocking mechanism, is uniformly mixed cell with pyrrole Luo Hong, pyrrole Luo Hong is set to rapidly enter the inside of cell, and stocking mechanism shakes back and forth, cell is moved back and forth smooth stocking mechanism slight internal, avoids clasmatosis.This method reduces the percentage of damage of cell by using the cellular elements agitating device of low sporoderm-broken rate, to improve the success rate of cellular assay.
Description
Technical field
The present invention relates to cell detection field, specifically a kind of RNA cellular elements method of inspection.
Background technique
Ribonucleic acid (is abbreviated as RNA), and the hereditary information being present in biological cell and fractionated viral, viroid carries
Body.RNA forms long chain molecule through phosphodiester bond condensation by ribonucleotide.One ribonucleic acid molecule is by phosphoric acid, core
Sugar and base are constituted.The base of RNA mainly has 4 kinds, i.e. A adenine, G guanine, C cytimidine, U uracil.
When detecting the RNA of cell interior, need to dye RNA using pyrrole Luo Hong；Pyrrole Luo Hong and mixing with cells exist
It needs to be stirred together, so that pyrrole Luo Hong is rapidly entered the inside of cell, but some rabbling mechanism velocities of rotation are excessive, easily lead to
Cell fragmentation reduces cell sample.
Summary of the invention
For the problems of the prior art, the present invention provides a kind of RNA cellular elements method of inspection, this method is by adopting
It is mixed with the cellular elements agitating device with low sporoderm-broken rate, reduces the percentage of damage of cell, to improve thin
The success rate that born of the same parents examine.
The technical solution adopted by the present invention to solve the technical problems is: a kind of RNA cellular elements method of inspection, this method
Including mixing RNA cellular elements in glass container with pyrrole Luo Hong, then by the cellular elements of the low sporoderm-broken rate of mixed liquor method
It carries out being sufficiently mixed dyeing in agitating device；
The cellular elements agitating device for the low sporoderm-broken rate that this method uses includes supporting table, driving mechanism, feeding mechanism, storage
Deposit mechanism, cutting agency, fixed mechanism, percussion mechanism and rubber block；The top surface of the supporting table is fixedly connected with the storage machine
Structure, the feeding mechanism is installed in the top surface of the stocking mechanism, and the cutting agency, institute are installed in the bottom end of the stocking mechanism
State the top surface of cutting agency installation and the supporting table；The internal slide of the stocking mechanism connects the fixed mechanism, and institute
The inner rotation for stating stocking mechanism connects the percussion mechanism, and the inner rotation of the percussion mechanism and the driving mechanism connects
It connects, and the driving mechanism is installed on the side wall of the stocking mechanism；The inner part of the stocking mechanism and the fixed mechanism
The rubber block is not installed.
Specifically, the stocking mechanism includes card slot, convex block, the first cylinder and the second cylinder, the top surface of the supporting table
It is fixedly connected with first cylinder, the internal slide of first cylinder connects second cylinder；Bottom end side wall is arc
The bottom surface of second cylinder is symmetrically installed the semicircular convex block, and it is " T " that the bottom end of first cylinder, which is equipped with sectional area,
The internal slide of the card slot of shape, the card slot connects the convex block.
Specifically, the feeding mechanism includes the first valve, loading hopper and the first bellows, the top of first cylinder
The loading hopper is installed in face, and the side wall of the loading hopper installs first valve；It installs the bottom end of the loading hopper
First bellows, first bellows connect the top surface of second cylinder through first cylinder.
Specifically, the cutting agency includes drainpipe, the second valve, the second bellows, fixed ring and groove, the branch
The inside for supportting platform is equipped with the groove, and one end of the groove is connected to the card slot；The inside of the groove and card slot peace
Second bellows that side wall is L shape is filled, the top of second bellows is installed on the top of second cylinder, institute
The drainpipe is installed in the bottom end for stating the second bellows, and the side wall of the drainpipe installs second valve；The groove
Inside the fixed ring is installed, the fixed ring is fixedly connected with second bellows；Symmetrically pacify the turning point of the card slot
The rubber block is filled, the side wall of the rubber block contradicts the side wall of second bellows.
Specifically, the driving mechanism includes motor and fixed case, the fixed case is fixed on the side of first cylinder
Wall, and the inside of the fixed case is fixedly connected with the motor.
Specifically, the percussion mechanism includes second spring, connecting rod, support frame, crank, connector sleeve and cutting ferrule, described
The second spring, the top of second cylinder are symmetrically installed between the inner sidewall of one cylinder and the side wall of second cylinder
Side wall installs support frame as described above, and support frame as described above is rotatablely connected the connecting rod；The link rotatable connects the connector sleeve, described
Connector sleeve is rotatablely connected the crank, and the crank is rotatablely connected the motor；Described in the sidewall symmetry installation of the crank
Cutting ferrule installs the connector sleeve between two cutting ferrules.
Specifically, the fixed mechanism includes fixed link, fixing sleeve, the first spring, sliding slot and ball, second cylinder
Top side wall be fixedly connected with the fixing sleeve, the both ends internal symmetry of the fixing sleeve installs multiple sliding slots, the cunning
The internal slide of slot connects the fixed link, and first bullet is installed between one end of the fixed link and one end of the sliding slot
Spring；The other end of the fixed link is rotatablely connected the ball, and the ball contradicts the inner sidewall of first cylinder；It is described solid
Surely the most short vertical range at the sidewall edge covered between the inner sidewall of first cylinder is greater than the height of the crank, and
The other end of the sliding slot is symmetrically installed the rubber block, and the rubber block contradicts the side wall of the fixed link.
Beneficial effects of the present invention:
(1) this method reduces the percentage of damage of cell by using the cellular elements agitating device of low sporoderm-broken rate, to mention
The high success rate of cellular assay.
(2) percussion mechanism, percussion mechanism peace are installed in the cellular elements agitating device that this method uses, the inside of stocking mechanism
Driving mechanism is filled, driving mechanism drives percussion mechanism to move back and forth, so that percussion mechanism be made to drive the shaking of stocking mechanism, makes thin
Born of the same parents are uniformly mixed with pyrrole Luo Hong, so that pyrrole Luo Hong is rapidly entered the inside of cell, and stocking mechanism shakes back and forth, cell is smooth
Moving back and forth for stocking mechanism slight internal, avoids clasmatosis, so that people be facilitated to detect cell.
(3) cutting agency is installed in the cellular elements agitating device that this method uses, the bottom end of stocking mechanism, cutting agency with
Rubber block is installed in the junction of stocking mechanism, and in stocking mechanism vibration, stocking mechanism drives cutting agency between rubber block
It shakes, rubber block is cutting agency damping, and cutting agency is avoided to be badly shaken.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the overall structure diagram for the cellular elements agitating device that this method uses；
Fig. 2 is stocking mechanism schematic diagram of internal structure shown in FIG. 1；
Fig. 3 is driving mechanism schematic diagram of internal structure shown in FIG. 1；
Fig. 4 is fixed mechanism schematic diagram of internal structure shown in Fig. 2.
In figure: 1, supporting table, 2, driving mechanism, 21, motor, 22, fixed case, 3, feeding mechanism, the 31, first valve, 32,
Loading hopper, the 33, first bellows, 4, stocking mechanism, 41, card slot, 42, convex block, the 43, first cylinder, the 44, second cylinder, 5,
Cutting agency, 51, drainpipe, the 52, second valve, the 53, second bellows, 54, fixed ring, 55, groove, 6, fixed mechanism, 61,
Fixed link, 62, fixing sleeve, the 63, first spring, 64, sliding slot, 65, ball, 7, percussion mechanism, 71, second spring, 72, connecting rod,
73, support frame, 74, crank, 75, connector sleeve, 76, cutting ferrule, 8, rubber block.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
As Figure 1-Figure 4, a kind of RNA cellular elements method of inspection of the present invention, this method include by RNA cell
Molecule mixes in glass container with pyrrole Luo Hong, then will carry out in the cellular elements agitating device of the low sporoderm-broken rate of mixed liquor method
It is sufficiently mixed dyeing；
The cellular elements agitating device for the low sporoderm-broken rate that this method uses includes supporting table 1, driving mechanism 2, feeding mechanism
3, stocking mechanism 4, cutting agency 5, fixed mechanism 7, percussion mechanism 8 and rubber block 8；The top surface of the supporting table 1 is fixedly connected
The feeding mechanism 3, and the bottom end installation of the stocking mechanism 4 are installed in the stocking mechanism 4, the top surface of the stocking mechanism 4
The cutting agency 5, the top surface of cutting agency 5 installation and the supporting table 1；The internal slide of the stocking mechanism 4 connects
Connect the fixed mechanism 6, and the inner rotation of the stocking mechanism 4 connects the percussion mechanism 7, and the percussion mechanism 7 with
The inner rotation of the driving mechanism 2 connects, and the driving mechanism 2 is installed on the side wall of the stocking mechanism 4；The storage
The inside of mechanism 4 and the fixed mechanism 6 is installed by the rubber block 8 respectively.
Specifically, the stocking mechanism 4 includes card slot 41, convex block 42, the first cylinder 43 and the second cylinder 44, the support
The top surface of platform 1 is fixedly connected with first cylinder 43, and the internal slide of first cylinder 43 connects second cylinder 44；Bottom
End side wall is that the bottom surface of second cylinder 44 of arc is symmetrically installed the semicircular convex block 42, first cylinder 43
It is the " T " shaped card slot 41 that bottom end, which is equipped with sectional area, and the internal slide of the card slot 41 connects the convex block 42, for convenience
Second cylinder 44 first cylinder 43 inside shaking, second cylinder 44 shake when, second cylinder
44 drive the semicircular convex block 41 to move back and forth in the inside of the card slot 41, to reduce second cylinder 44 in institute
State frictional force when the first 43 internal motion of cylinder.
Specifically, the feeding mechanism 3 include the first valve 31, loading hopper 32 and the first bellows 33, described first
The loading hopper 32 is installed in the top surface of cylinder 43, and the side wall of the loading hopper 32 installs first valve 31；It is described into
First bellows 33 is installed in the bottom end of funnel 32, and first bellows 33 connects described through first cylinder 43
First valve 31 is opened in the top surface of second cylinder 44 for convenience, leaks cell sample and pyrrole Luo Hong by the charging
Bucket 32 enters the inside of second cylinder 44, and when second cylinder 44 shaking, drives first bellows 33 flexible
Second cylinder 44, is closely connect with the loading hopper 32 by first bellows 33, is facilitated to described by movement
Cell sample and pyrrole Luo Hong are added inside second cylinder 44.
Specifically, the cutting agency 5 includes drainpipe 51, the second valve 52, the second bellows 53, fixed ring 54 and recessed
Slot 55, the inside of the supporting table 1 are equipped with the groove 55, and one end of the groove 55 is connected to the card slot 41；The groove
55 and the card slot 41 inside installation side wall be L shape second bellows 53, the top of second bellows 53
It is installed on the top of second cylinder 44, the drainpipe 51, and the draining are installed in the bottom end of second bellows 44
The side wall of pipe 51 installs second valve 52；In order to facilitate opening second valve 52, inside second cylinder 44
Mixed cell discharge；The fixed ring 54 is installed in the inside of the groove 55, and the fixed ring 54 is fixedly connected with described the
Two bellowss 53；Second bellows 53 is fixed on to the inside of the supporting table 1 for convenience；The turnover of the card slot 41
Place is symmetrically installed the rubber block 8, and the side wall of the rubber block 8 contradicts the side wall of second bellows 53, described second
When cylinder 44 shakes, second cylinder 44 drives second bellows 53 to shake between the rubber block 8, the rubber
Block 8 is 53 damping of the second bellows, and second bellows 53 is avoided to damage.
Specifically, the driving mechanism 2 includes motor 21 and fixed case 22, the fixed case 22 is fixed on described first
The side wall of body 43, and the inside of the fixed case 22 is fixedly connected with the motor 21, the motor 21 is described solid for convenience
Determine the inner rotation of case 22.
Specifically, the percussion mechanism 7 includes second spring 71, connecting rod 72, support frame 73, crank 74,75 and of connector sleeve
Cutting ferrule 76 is symmetrically installed the second spring between the inner sidewall of first cylinder 43 and the side wall of second cylinder 44
71, the top side wall of second cylinder 44 installs support frame as described above 73, and support frame as described above 73 is rotatablely connected the connecting rod 72；Institute
It states connecting rod 72 and is rotatablely connected the connector sleeve 75, the connector sleeve 75 is rotatablely connected the crank 74, and the crank 74 rotates
Connect the motor 21；The sidewall symmetry of the crank 74 installs the cutting ferrule 76, between two cutting ferrules 76 described in installation
Connector sleeve 75, in order to which the connecting rod 72 to be fixed on the crank 74, the crank 74 rotation for convenience drives the company
Female connector 75 and the connecting rod 72 move back and forth, so that the connecting rod 72 be made to drive support frame as described above 73 and second cylinder 44
It returns and shakes, thus mix cell and pyrrole the Luo Hong mixing inside second cylinder 44, and when second cylinder 44 shaking,
One end of second cylinder 44 squeezes the second spring 71, and the other end of second cylinder 44 elongates the second spring
71, make the second spring 71 second cylinder, 44 damping, avoids second cylinder 44 from acutely shaking, cell is avoided to damage
It is bad.
Specifically, the fixed mechanism 6 includes fixed link 61, fixing sleeve 62, the first spring 63, sliding slot 64 and ball 65,
The top side wall of second cylinder 44 is fixedly connected with the fixing sleeve 62, and the both ends internal symmetry installation of the fixing sleeve 62 is more
A sliding slot 64, the internal slide of the sliding slot 64 connect the fixed link 61, one end of fixed link 61 described in side with it is described
First spring 63 is installed between one end of sliding slot 64；The other end of the fixed link 61 is rotatablely connected the ball 65, institute
State the inner sidewall that ball 65 contradicts first cylinder 43；When second cylinder 44 shakes for convenience, the fixing sleeve 62
First spring 63 elongation inside one end skids off the fixed link 61 from the inside of the sliding slot 64, to make described solid
The ball 65 of 61 one end of fixed pole contradicts one end inner sidewall of first cylinder 43, and 62 other end of fixing sleeve compresses institute
Fixed link 61 is stated, so that the fixed link 61 is entered the inside of the sliding slot 64, the fixed link 61 is made to contradict first cylinder
43 other end inner sidewall so that the fixed link 61 be made to fix the top of second cylinder 44, and reduces described second
The shaking degree of cylinder 44；It is most short vertical between the inner sidewall of first cylinder 43 at the sidewall edge of the fixing sleeve 62
Straight distance is greater than the height of the crank 74 and avoids institute when the connecting rod 72 drives second cylinder 44 to shake for convenience
State the inner sidewall that fixing sleeve 62 contradicts first cylinder 43；And the other end of the sliding slot 64 is symmetrically installed the rubber block 8,
The rubber block 8 contradicts the side wall of the fixed link 61, and the rubber block 8 consolidates the side wall of the fixed link 61 for convenience
It is fixed, facilitate the fixed link 61 in the internal slide of the sliding slot 64.
When in use, the present apparatus is accessed into power supply, opens the first valve 31, closed the second valve 52, pass through loading hopper
32 are added suitable cell sample and pyrrole Luo Hong, and cell sample and pyrrole Luo Hong enter through loading hopper 32 and the first bellows 33
The inside of second cylinder 44, then the first valve 31 is closed.Open motor 21, inner rotation of the motor 21 in fixed case 22, electricity
Machine 21 drives crank 74 to rotate, and crank 74 drives connector sleeve 75 to move up and down, and connector sleeve 75 is in crank 74 and two cutting ferrules 76
Between rotate, the movement of connector sleeve 75 drives connecting rod 72 to move back and forth, and one end of connecting rod 72 is rotated in the side wall of support frame 73, and even
Bar 72 drives the second cylinder 44 to move back and forth in the inside of the first cylinder 43, makes cell sample and pyrrole Luo Hong in the second cylinder 44
Internal mix is uniform, and pyrrole Luo Hong is facilitated to enter cell interior, dyes pyrrole Luo Hong RNA.When the second cylinder 44 is in the first cylinder
When 43 internal motion, the second cylinder 44 drives semicircular convex block 41 to move back and forth in the inside of card slot 41, to reduce the
Frictional force of two cylinders 44 in the first 43 internal motion of cylinder, and top the first bellows 33 of drive of the second cylinder 44 does and stretches
Contracting movement makes to connect between loading hopper 32 and the second cylinder 44 by the first bellows 33, the top of the second cylinder 44 is sealed
It closes, cell sample and pyrrole Luo Hong is avoided to spill from the top surface of the second cylinder 44；Second cylinder 44 drives the second bellows 53 two
It is shaken between a rubber block 8, the second bellows 53 squeezes rubber block 8,8 the second bellows of reverse extrusion 53 of rubber block, by second
Bellows 53 is fixed on the inside of card slot 41, and the second bellows 53 is fixed, and rubber block 8 subtracts the top of the second bellows 53
Shake, the bottom end of the second bellows 53 are fixed the inside that ring 54 is fixed on groove 55, the second bellows 53 are both avoided to be damaged, and
The top of the second bellows 53 is set to be firmly fixed to the bottom end of the second cylinder 44.In the second cylinder 44 in the first cylinder 43
When portion is slided, one end of the second cylinder 44 squeezes the second spring 71 for being located at 43 one end of the first cylinder, the second cylinder 44 it is another
The second spring 71 for being located at 43 other end of the first cylinder is elongated at end, and the side wall of the second cylinder 44 wrapped up by second spring 71, makes the
Two springs 71 are 44 damping of the second cylinder, avoid the second cylinder 44 from acutely shaking, avoid cell damage；And second cylinder 44 drive
Fixing sleeve 62 shakes, make the first spring 63 inside 62 one end of fixing sleeve extend make fixed link 61 positioned at 62 one end of fixing sleeve from
The inside of sliding slot 64 skids off, to make one end inner sidewall of first cylinder 43 of conflict of ball 65 of 61 one end of fixed link, fixing sleeve
62 other ends compress fixed link 61, make to be located at 62 other end of fixing sleeve to be the inside that fixed link 61 enters sliding slot 64, fixed link 61
The first spring 63 is compressed, so that ball 65 is contradicted the other end inner sidewall of the first cylinder 43, ball 65 is in the inside of the first cylinder 43
Rotation reduces the frictional force between ball 65 and 43 inner sidewall of the first cylinder, and ball 65 is facilitated to transport in the inside of the first cylinder 43
It is dynamic, to make fixed link 61 and fixing sleeve 63 fix the top of the second cylinder 44, and different the first springs of compression and elongation
63 reduce the shaking degree of the second cylinder 44；It is most short between the inner sidewall of the first cylinder 43 at the sidewall edge of fixing sleeve 62
Vertical range is greater than the height of crank 74, when connecting rod 72 drives the second cylinder 44 to shake, fixing sleeve 62 is avoided to contradict first
The inner sidewall of body 43；And the other end of sliding slot 64 is symmetrically installed rubber block 8, rubber block 8 contradicts the side wall of fixed link 61, makes rubber
Block 8 fixes the side wall of fixed link 61, facilitates fixed link 61 in the internal slide of sliding slot 64.After mixing mixes, motor 21 is closed,
Open the second valve, 52, the cell sample and pyrrole Luo Hong discharge that water mixing mixes.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the description in above embodiment and specification only illustrates the present invention
Principle, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these variation and
Improvement is both fallen in the scope of protection of present invention.The claimed scope of the invention is by appended claims and its equivalent
Object defines.
Claims (7)
1. a kind of RNA cellular elements method of inspection, which is characterized in that this method includes by RNA cellular elements and pyrrole Luo Hong in glass
It is mixed in glass container, then will carry out being sufficiently mixed dyeing in the cellular elements agitating device of the low sporoderm-broken rate of mixed liquor method；
The cellular elements agitating device for the low sporoderm-broken rate that this method uses includes supporting table (1), driving mechanism (2), feeding mechanism
(3), stocking mechanism (4), cutting agency (5), fixed mechanism (7), percussion mechanism (8) and rubber block (8)；The supporting table (1)
Top surface be fixedly connected the stocking mechanism (4), the feeding mechanism (3) is installed in the top surface of the stocking mechanism (4), and described
The bottom end of stocking mechanism (4) is installed by the cutting agency (5), the top of cutting agency (5) installation and the supporting table (1)
Face；The internal slide of the stocking mechanism (4) connects the fixed mechanism (6), and the inner rotation of the stocking mechanism (4) connects
It connects the percussion mechanism (7), and the percussion mechanism (7) is connect with the inner rotation of the driving mechanism (2), and the driving
Mechanism (2) is installed on the side wall of the stocking mechanism (4)；The inside of the stocking mechanism (4) and the fixed mechanism (6) difference
The rubber block (8) are installed.
2. a kind of RNA cellular elements method of inspection according to claim 1, it is characterised in that: the stocking mechanism (4) includes card
Slot (41), convex block (42), the first cylinder (43) and the second cylinder (44), the top surface of the supporting table (1) are fixedly connected with described
The internal slide of one cylinder (43), first cylinder (43) connects second cylinder (44)；Bottom end side wall for arc institute
The bottom surface for stating the second cylinder (44) is symmetrically installed the semicircular convex block (42), and the bottom end of first cylinder (43), which is equipped with, to be cut
Area is the " T " shaped card slot (41), and the internal slide of the card slot (41) connects the convex block (42).
3. a kind of RNA cellular elements method of inspection according to claim 2, it is characterised in that: feeding mechanism (3) packet
Include the first valve (31), loading hopper (32) and the first bellows (33), the top surface installation of first cylinder (43) it is described into
The side wall of funnel (32), the loading hopper (32) installs first valve (31)；The bottom end of the loading hopper (32)
First bellows (33) is installed, first bellows (33) connects second cylinder through first cylinder (43)
(44) top surface.
4. a kind of RNA cellular elements method of inspection according to claim 3, it is characterised in that: cutting agency (5) packet
Drainpipe (51), the second valve (52), the second bellows (53), fixed ring (54) and groove (55) are included, the supporting table (1)
Inside is equipped with the groove (55), and one end of the groove (55) is connected to the card slot (41)；The groove (55) and the card
The inside installation side wall of slot (41) is second bellows (53) of L shape, the top installation of second bellows (53)
In the top of second cylinder (44), the drainpipe (51), and the row are installed in the bottom end of second bellows (44)
The side wall of water pipe (51) installs second valve (52)；The fixed ring (54) is installed in the inside of the groove (55), described
Fixed ring (54) is fixedly connected with second bellows (53)；The turning point of the card slot (41) is symmetrically installed the rubber block
(8), the side wall of the rubber block (8) contradicts the side wall of second bellows (53).
5. a kind of RNA cellular elements method of inspection according to claim 2, it is characterised in that: driving mechanism (2) packet
Motor (21) and fixed case (22) are included, the fixed case (22) is fixed on the side wall of first cylinder (43), and the fixation
The inside of case (22) is fixedly connected with the motor (21).
6. a kind of RNA cellular elements method of inspection according to claim 5, it is characterised in that: percussion mechanism (7) packet
Include second spring (71), connecting rod (72), support frame (73), crank (74), connector sleeve (75) and cutting ferrule (76), first cylinder
(43) it is symmetrically installed the second spring (71) between the side wall of inner sidewall and second cylinder (44), second cylinder
(44) top side wall installs support frame as described above (73), and support frame as described above (73) is rotatablely connected the connecting rod (72)；The connecting rod
(72) it is rotatablely connected the connector sleeve (75), the connector sleeve (75) is rotatablely connected the crank (74), and the crank (74)
It is rotatablely connected the motor (21)；The sidewall symmetry of the crank (74) installs the cutting ferrule (76), two cutting ferrules (76)
Between the connector sleeve (75) are installed.
7. a kind of RNA cellular elements method of inspection according to claim 6, it is characterised in that: fixed mechanism (6) packet
Include fixed link (61), fixing sleeve (62), the first spring (63), sliding slot (64) and ball (65), the top of second cylinder (44)
End side wall is fixedly connected the fixing sleeve (62), and the both ends internal symmetry of the fixing sleeve (62) installs multiple sliding slots
(64), the internal slide of the sliding slot (64) connects the fixed link (61), one end of fixed link (61) described in side and the cunning
First spring (63) is installed between one end of slot (64)；The other end of the fixed link (61) is rotatablely connected the ball
(65), the ball (65) contradicts the inner sidewall of first cylinder (43)；At the sidewall edge of the fixing sleeve (62) and institute
State the height that the most short vertical range between the inner sidewall of the first cylinder (43) is greater than the crank (74), and the sliding slot (64)
The other end be symmetrically installed the rubber block (8), the rubber block (8) contradicts the side wall of the fixed link (61).
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| CN201811236925.0A CN109321632B (en) | 2018-10-23 | 2018-10-23 | RNA cell molecule inspection method |
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| CN109321632B (en) | 2021-07-27 |
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Denomination of invention: A Method for RNA Cell Molecular Testing Granted publication date: 20210727 Pledgee: Beijing Bank Co.,Ltd. Beiyuan Road Branch Pledgor: BEIJING GENE LINE HUAKE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024990000136 |
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