CN109321632B - RNA cell molecule inspection method - Google Patents
RNA cell molecule inspection method Download PDFInfo
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- CN109321632B CN109321632B CN201811236925.0A CN201811236925A CN109321632B CN 109321632 B CN109321632 B CN 109321632B CN 201811236925 A CN201811236925 A CN 201811236925A CN 109321632 B CN109321632 B CN 109321632B
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- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000007689 inspection Methods 0.000 title abstract description 7
- 230000007246 mechanism Effects 0.000 claims abstract description 124
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 239000011521 glass Substances 0.000 claims abstract description 4
- 238000004043 dyeing Methods 0.000 claims abstract description 3
- 238000009434 installation Methods 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 abstract description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
- 239000011435 rock Substances 0.000 description 7
- 108091028664 Ribonucleotide Proteins 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- SIXLXDIJGIWWFU-UHFFFAOYSA-N pyritinol Chemical compound OCC1=C(O)C(C)=NC=C1CSSCC1=CN=C(C)C(O)=C1CO SIXLXDIJGIWWFU-UHFFFAOYSA-N 0.000 description 2
- 229960004986 pyritinol Drugs 0.000 description 2
- 239000002336 ribonucleotide Substances 0.000 description 2
- 125000002652 ribonucleotide group Chemical group 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 241000726445 Viroids Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The invention relates to the field of cell detection, in particular to a method for detecting RNA cell molecules, which comprises the steps of mixing RNA cell molecules and pirocin in a glass container, and then fully mixing and dyeing the mixture in a cell molecule stirring device with low wall-breaking rate; the cell molecule stirring device with the low wall breaking rate comprises a storage mechanism, a vibration mechanism is installed inside the storage mechanism, a driving mechanism is installed on the vibration mechanism, and the driving mechanism drives the vibration mechanism to move back and forth, so that the vibration mechanism drives the storage mechanism to shake, cells and the pirocin are uniformly mixed, the pirocin can quickly enter the inside of the cells, the storage mechanism shakes back and forth, and the cells slightly move back and forth inside the smooth storage mechanism, so that the cells are prevented from being broken. The method reduces the breakage rate of cells by adopting a cell molecule stirring device with low wall breaking rate, thereby improving the success rate of cell inspection.
Description
Technical Field
The invention relates to the field of cell detection, in particular to a RNA cell molecule detection method.
Background
Ribonucleic acids (abbreviated as RNA), genetic information carriers present in biological cells and in parts of viruses, viroids. RNA is a long chain molecule formed by the condensation of ribonucleotides via phosphodiester bonds. One ribonucleotide molecule consists of a phosphate, a ribose and a base. RNA has 4 kinds of bases, namely A adenine, G guanine, C cytosine and U uracil.
When RNA inside cells is detected, the RNA needs to be stained by using the pirocin; the mixture of the pirocin and the cells needs to be stirred, so that the pirocin can rapidly enter the cells, but some stirring mechanisms have excessive rotating speed, so that the cells are easy to crack, and the cell samples are reduced.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an RNA cell molecule inspection method, which reduces the breakage rate of cells by adopting a cell molecule stirring device with low wall-breaking rate to carry out mixing and stirring, thereby improving the success rate of cell inspection.
The technical scheme adopted by the invention for solving the technical problems is as follows: a method for detecting RNA cell molecules comprises the steps of mixing RNA cell molecules and pirocin in a glass container, and then fully mixing and dyeing the mixture in a cell molecule stirring device with low wall-breaking rate;
the cell molecule stirring device with low wall breaking rate adopted by the method comprises a supporting table, a driving mechanism, a feeding mechanism, a storage mechanism, a blanking mechanism, a fixing mechanism, a vibrating mechanism and a rubber block; the top surface of the supporting table is fixedly connected with the storage mechanism, the feeding mechanism is installed on the top surface of the storage mechanism, the blanking mechanism is installed at the bottom end of the storage mechanism, and the blanking mechanism is installed on the top surface of the supporting table; the inner part of the storage mechanism is connected with the fixing mechanism in a sliding manner, the inner part of the storage mechanism is connected with the vibration mechanism in a rotating manner, the vibration mechanism is connected with the inner part of the driving mechanism in a rotating manner, and the driving mechanism is arranged on the side wall of the storage mechanism; the storage mechanism and the fixing mechanism are internally provided with the rubber blocks respectively.
Specifically, the storage mechanism comprises a clamping groove, a convex block, a first cylinder and a second cylinder, the top surface of the supporting table is fixedly connected with the first cylinder, and the interior of the first cylinder is connected with the second cylinder in a sliding manner; the bottom side wall of the bottom end is arc-shaped, the bottom surface of the second barrel is symmetrically provided with semicircular lugs, the bottom end of the first barrel is provided with a clamping groove with a T-shaped section area, and the inside of the clamping groove is in sliding connection with the lugs.
Specifically, the feeding mechanism comprises a first valve, a feeding funnel and a first corrugated pipe, the feeding funnel is mounted on the top surface of the first cylinder, and the first valve is mounted on the side wall of the feeding funnel; the bottom end of the feeding funnel is provided with the first corrugated pipe, and the first corrugated pipe penetrates through the first cylinder body and is connected with the top surface of the second cylinder body.
Specifically, the blanking mechanism comprises a drain pipe, a second valve, a second corrugated pipe, a fixing ring and a groove, the groove is formed in the supporting table, and one end of the groove is communicated with the clamping groove; the second corrugated pipe with L-shaped side walls is arranged in the groove and the clamping groove, the top end of the second corrugated pipe is arranged at the top end of the second cylinder, the drain pipe is arranged at the bottom end of the second corrugated pipe, and the second valve is arranged on the side wall of the drain pipe; the fixing ring is arranged in the groove and fixedly connected with the second corrugated pipe; the turning point of the clamping groove is symmetrically provided with the rubber block, and the side wall of the rubber block is abutted against the side wall of the second corrugated pipe.
Specifically, actuating mechanism includes motor and fixed case, fixed case is fixed in the lateral wall of first barrel, just the inside fixed connection of fixed case the motor.
Specifically, the vibration mechanism comprises a second spring, a connecting rod, a support frame, a crank, a connecting sleeve and a clamping sleeve, the second spring is symmetrically arranged between the inner side wall of the first cylinder and the side wall of the second cylinder, the support frame is arranged on the side wall of the top end of the second cylinder, and the support frame is rotatably connected with the connecting rod; the connecting rod is rotationally connected with the connecting sleeve, the connecting sleeve is rotationally connected with the crank, and the crank is rotationally connected with the motor; the clamping sleeves are symmetrically arranged on the side wall of the crank, and the connecting sleeve is arranged between the two clamping sleeves.
Specifically, the fixing mechanism comprises a fixing rod, a fixing sleeve, a first spring, sliding chutes and balls, the side wall of the top end of the second cylinder is fixedly connected with the fixing sleeve, a plurality of sliding chutes are symmetrically arranged in two ends of the fixing sleeve, the fixing rod is connected in the sliding chutes in a sliding manner, and the first spring is arranged between one end of the fixing rod and one end of the sliding chute; the other end of the fixed rod is rotatably connected with the ball, and the ball is abutted against the inner side wall of the first cylinder; the utility model discloses a fixed cover, including fixed cover, first barrel, the lateral wall edge of fixed cover with the shortest vertical distance between the inside wall of first barrel is greater than articulate height, just the other end symmetry installation of spout the block rubber, the block rubber is contradicted the lateral wall of dead lever.
The invention has the beneficial effects that:
(1) the method reduces the breakage rate of cells by adopting a cell molecule stirring device with low wall breaking rate, thereby improving the success rate of cell inspection.
(2) According to the cell molecule stirring device adopted by the method, the vibration mechanism is arranged inside the storage mechanism, the driving mechanism is arranged on the vibration mechanism, and the driving mechanism drives the vibration mechanism to move back and forth, so that the vibration mechanism drives the storage mechanism to shake, cells and the pirocin are uniformly mixed, the pirocin can quickly enter the inside of the cells, the storage mechanism shakes back and forth, the cells slightly move back and forth inside the smooth storage mechanism, cell breakage is avoided, and people can conveniently detect the cells.
(3) According to the cell molecule stirring device adopted by the method, the blanking mechanism is installed at the bottom end of the storage mechanism, the rubber block is installed at the joint of the blanking mechanism and the storage mechanism, when the storage mechanism vibrates, the storage mechanism drives the blanking mechanism to shake between the rubber blocks, the rubber blocks are used for damping the blanking mechanism, and the blanking mechanism is prevented from being damaged by vibration.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic diagram of the overall structure of a cell molecule stirring device used in the method;
FIG. 2 is a schematic view of the internal structure of the storage mechanism shown in FIG. 1;
FIG. 3 is a schematic view of the internal structure of the driving mechanism shown in FIG. 1;
fig. 4 is a schematic view of the internal structure of the fixing mechanism shown in fig. 2.
In the figure: 1. supporting table, 2, driving mechanism, 21, motor, 22, fixed box, 3, feeding mechanism, 31, first valve, 32, feeding funnel, 33, first bellows, 4, storage mechanism, 41, clamping groove, 42, bump, 43, first cylinder, 44, second cylinder, 5, blanking mechanism, 51, drain pipe, 52, second valve, 53, second bellows, 54, fixed ring, 55, groove, 6, fixing mechanism, 61, fixed rod, 62, fixed sleeve, 63, first spring, 64, chute, 65, ball, 7, vibrating mechanism, 71, second spring, 72, connecting rod, 73, supporting frame, 74, crank, 75, connecting sleeve, 76, clamping sleeve, 8, rubber block.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1-4, the RNA cell molecule inspection method of the present invention comprises mixing RNA cell molecules with pirocin in a glass container, and then fully mixing and staining the mixture in a cell molecule stirring device with low wall-breaking rate;
the cell molecule stirring device with low wall breaking rate adopted by the method comprises a support table 1, a driving mechanism 2, a feeding mechanism 3, a storage mechanism 4, a blanking mechanism 5, a fixing mechanism 7, a vibrating mechanism 8 and a rubber block 8; the top surface of the supporting table 1 is fixedly connected with the storage mechanism 4, the feeding mechanism 3 is installed on the top surface of the storage mechanism 4, the blanking mechanism 5 is installed at the bottom end of the storage mechanism 4, and the blanking mechanism 5 is installed on the top surface of the supporting table 1; the inner part of the storage mechanism 4 is slidably connected with the fixing mechanism 6, the inner part of the storage mechanism 4 is rotatably connected with the vibration mechanism 7, the vibration mechanism 7 is rotatably connected with the inner part of the driving mechanism 2, and the driving mechanism 2 is arranged on the side wall of the storage mechanism 4; the rubber blocks 8 are respectively arranged inside the storage mechanism 4 and the fixing mechanism 6.
Specifically, the storage mechanism 4 includes a slot 41, a protrusion 42, a first cylinder 43 and a second cylinder 44, the top surface of the support table 1 is fixedly connected to the first cylinder 43, and the inside of the first cylinder 43 is slidably connected to the second cylinder 44; the semicircular lug 42 is symmetrically installed on the bottom surface of the second cylinder 44 with the arc-shaped bottom end side wall, the clamping groove 41 with the T-shaped cross section is arranged at the bottom end of the first cylinder 43, the lug 42 is slidably connected inside the clamping groove 41, so that the second cylinder 44 can conveniently shake inside the first cylinder 43, and when the second cylinder 44 shakes, the second cylinder 44 drives the semicircular lug 41 to move back and forth inside the clamping groove 41, and friction force of the second cylinder 44 during movement inside the first cylinder 43 is reduced.
Specifically, the feeding mechanism 3 includes a first valve 31, a feeding funnel 32 and a first bellows 33, the feeding funnel 32 is mounted on the top surface of the first cylinder 43, and the first valve 31 is mounted on the side wall of the feeding funnel 32; the bottom installation of feed hopper 32 first bellows 33, first bellows 33 runs through first barrel 43 is connected the top surface of second barrel 44, for the convenience open first valve 31 makes cell sample and pirocin pass through feed hopper 32 gets into the inside of second barrel 44, just when second barrel 44 rocks, drives first bellows 33 concertina movement, through first bellows 33 will second barrel 44 with feed hopper 32 zonulae occludens, the convenience to inside cell sample and the pirocin of adding of second barrel 44.
Specifically, the blanking mechanism 5 comprises a drain pipe 51, a second valve 52, a second corrugated pipe 53, a fixing ring 54 and a groove 55, the groove 55 is arranged inside the support table 1, and one end of the groove 55 is communicated with the clamping groove 41; the groove 55 and the second corrugated tube 53 with an L-shaped inner side wall are mounted in the clamping groove 41, the top end of the second corrugated tube 53 is mounted at the top end of the second cylinder 44, the bottom end of the second corrugated tube 44 is mounted with the drain pipe 51, and the side wall of the drain pipe 51 is mounted with the second valve 52; discharging the mixed cells inside the second cylinder 44 in order to open the second valve 52; the fixing ring 54 is installed inside the groove 55, and the fixing ring 54 is fixedly connected with the second corrugated pipe 53; to facilitate fixing the second bellows 53 inside the support table 1; the symmetry installation of turning department of draw-in groove 41 block rubber 8, the lateral wall of block rubber 8 is contradicted the lateral wall of second bellows 53 when second barrel 44 rocks, second barrel 44 drives second bellows 53 is in rock between the block rubber 8, block rubber 8 does the shock attenuation of second bellows 53 is avoided second bellows 53 damages.
Specifically, the driving mechanism 2 includes a motor 21 and a fixed box 22, the fixed box 22 is fixed on the side wall of the first cylinder 43, and the inside of the fixed box 22 is fixedly connected to the motor 21, so as to facilitate the rotation of the motor 21 in the fixed box 22.
Specifically, the vibration mechanism 7 includes a second spring 71, a connecting rod 72, a supporting frame 73, a crank 74, a connecting sleeve 75 and a sleeve 76, the second spring 71 is symmetrically installed between the inner side wall of the first cylinder 43 and the side wall of the second cylinder 44, the supporting frame 73 is installed on the side wall of the top end of the second cylinder 44, and the supporting frame 73 is rotatably connected with the connecting rod 72; the connecting rod 72 is rotatably connected with the connecting sleeve 75, the connecting sleeve 75 is rotatably connected with the crank 74, and the crank 74 is rotatably connected with the motor 21; the lateral wall symmetry installation of crank 74 the cutting ferrule 76, two install between the cutting ferrule 76 the adapter sleeve 75, in order to incite somebody to action the connecting rod 72 is fixed on the crank 74, for the convenience the crank 74 rotates the drive the adapter sleeve 75 with connecting rod 72 back and forth movement, thereby makes the connecting rod 72 drive the support frame 73 with the second barrel 44 is made a round trip to rock, thereby makes the inside cell of second barrel 44 and the mixed mixing of pyroracemic mixture, just when the second barrel 44 rocks, the one end extrusion of second barrel 44 the second spring 71, the other end of second barrel 44 is elongated the second spring 71, makes the second spring 71 does the shock attenuation of second barrel 44 avoids the second barrel 44 acutely rocks, avoids cell damage.
Specifically, the fixing mechanism 6 includes a fixing rod 61, a fixing sleeve 62, a first spring 63, a sliding groove 64 and a ball 65, the side wall of the top end of the second cylinder 44 is fixedly connected to the fixing sleeve 62, a plurality of sliding grooves 64 are symmetrically installed inside two ends of the fixing sleeve 62, the inside of the sliding groove 64 is slidably connected to the fixing rod 61, and the first spring 63 is installed between one end of the side fixing rod 61 and one end of the sliding groove 64; the other end of the fixing rod 61 is rotatably connected with the ball 65, and the ball 65 is abutted against the inner side wall of the first cylinder 43; in order to facilitate the shaking of the second cylinder 44, the first spring 63 inside one end of the fixing sleeve 62 is extended to slide the fixing rod 61 out of the sliding slot 64, so that the ball 65 at one end of the fixing rod 61 abuts against the inner side wall at one end of the first cylinder 43, the other end of the fixing sleeve 62 compresses the fixing rod 61, the fixing rod 61 enters the sliding slot 64, the fixing rod 61 abuts against the inner side wall at the other end of the first cylinder 43, so that the fixing rod 61 fixes the top end of the second cylinder 44, and the shaking degree of the second cylinder 44 is reduced; the shortest vertical distance between the edge of the side wall of the fixing sleeve 62 and the inner side wall of the first cylinder 43 is greater than the height of the crank 74, so that when the connecting rod 72 drives the second cylinder 44 to swing, the fixing sleeve 62 is prevented from abutting against the inner side wall of the first cylinder 43; and the other end symmetry installation of spout 64 block 8, block 8 contradicts the lateral wall of dead lever 61, for the convenience block 8 will the lateral wall of dead lever 61 is fixed, and is convenient dead lever 61 is in the inside slip of spout 64.
When the device is used, the power supply is switched on, the first valve 31 is opened, the second valve 52 is closed, a proper amount of cell sample and pyritinol are added through the feeding funnel 32, the cell sample and the pyritinol penetrate through the feeding funnel 32 and the first corrugated pipe 33 to enter the inside of the second cylinder 44, and then the first valve 31 is closed. The motor 21 is turned on, the motor 21 rotates in the fixed box 22, the motor 21 drives the crank 74 to rotate, the crank 74 drives the connecting sleeve 75 to move up and down, the connecting sleeve 75 rotates between the crank 74 and the two clamping sleeves 76, the connecting sleeve 75 drives the connecting rod 72 to move back and forth, one end of the connecting rod 72 rotates on the side wall of the supporting frame 73, and the connecting rod 72 drives the second cylinder 44 to move back and forth in the first cylinder 43, so that the cell sample and the pyrrocin are uniformly mixed in the second cylinder 44, the pyrrocin can conveniently enter the cell, and the pyrrocin can be dyed by RNA. When the second cylinder 44 moves inside the first cylinder 43, the second cylinder 44 drives the semicircular protrusion 41 to move back and forth inside the slot 41, so as to reduce the friction force of the second cylinder 44 moving inside the first cylinder 43, and the top end of the second cylinder 44 drives the first bellows 33 to move in a telescopic manner, so that the feeding funnel 32 is connected with the second cylinder 44 through the first bellows 33, the top end of the second cylinder 44 is sealed, and the cell sample and the pirrolin are prevented from spilling out of the top surface of the second cylinder 44; the second cylinder 44 drives the second bellows 53 to rock between the two rubber blocks 8, the second bellows 53 extrudes the rubber blocks 8, the rubber blocks 8 reversely extrude the second bellows 53, the second bellows 53 is fixed in the clamping groove 41, the second bellows 53 is fixed, the top end of the second bellows 53 is damped by the rubber blocks 8, the bottom end of the second bellows 53 is fixed in the groove 55 by the fixing ring 54, the second bellows 53 is prevented from being damaged, and the top end of the second bellows 53 is firmly fixed at the bottom end of the second cylinder 44. When the second cylinder 44 slides in the first cylinder 43, one end of the second cylinder 44 presses the second spring 71 at one end of the first cylinder 43, the other end of the second cylinder 44 elongates the second spring 71 at the other end of the first cylinder 43, and the side wall of the second cylinder 44 is wrapped by the second spring 71, so that the second spring 71 is used for damping the second cylinder 44, the second cylinder 44 is prevented from shaking violently, and the cells are prevented from being damaged; and the second cylinder 44 drives the fixing sleeve 62 to swing, so that the first spring 63 inside one end of the fixing sleeve 62 extends to slide the fixing rod 61 at one end of the fixing sleeve 62 out of the sliding slot 64, thereby the ball 65 at one end of the fixing rod 61 is abutted against the inner side wall at one end of the first cylinder 43, the other end of the fixing sleeve 62 compresses the fixing rod 61, the fixing rod 61 at the other end of the fixing sleeve 62 enters the inside of the chute 64, the fixing rod 61 compresses the first spring 63, the ball 65 is abutted against the inner side wall at the other end of the first cylinder 43, the ball 65 rotates inside the first cylinder 43, the friction between the ball 65 and the inner side wall of the first cylinder 43 is reduced, the ball 65 conveniently moves inside the first cylinder 43, thereby the fixing rod 61 and the fixing sleeve 63 fix the top end of the second cylinder 44, and the different compression and elongation of the first spring 63 reduce the shaking degree of the second cylinder 44; the shortest vertical distance between the edge of the side wall of the fixing sleeve 62 and the inner side wall of the first cylinder 43 is greater than the height of the crank 74, so that when the connecting rod 72 drives the second cylinder 44 to swing, the fixing sleeve 62 is prevented from abutting against the inner side wall of the first cylinder 43; and the other end symmetry installation block rubber 8 of spout 64, block rubber 8 supports the lateral wall of dead lever 61, makes block rubber 8 fix the lateral wall of dead lever 61, makes things convenient for dead lever 61 to slide in the inside of spout 64. After mixing, the motor 21 is turned off, the second valve 52 is opened, and the water is discharged to mix the mixed cell sample and the pirocin.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the embodiments and descriptions given above are only illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (1)
1. A method for detecting RNA cell molecules is characterized in that the method comprises the steps of mixing RNA cell molecules and pirocin in a glass container, and then putting the mixed solution into a cell molecule stirring device with low wall-breaking rate for full mixing and dyeing;
the cell molecule stirring device with low wall breaking rate adopted by the method comprises a supporting table (1), a driving mechanism (2), a feeding mechanism (3), a storage mechanism (4), a blanking mechanism (5), a fixing mechanism (6), a vibrating mechanism (7) and a rubber block (8); the top surface of the supporting table (1) is fixedly connected with the storage mechanism (4), the feeding mechanism (3) is installed on the top surface of the storage mechanism (4), the blanking mechanism (5) is installed at the bottom end of the storage mechanism (4), and the blanking mechanism (5) is installed on the top surface of the supporting table (1); the inner part of the storage mechanism (4) is connected with the fixing mechanism (6) in a sliding manner, the inner part of the storage mechanism (4) is connected with the vibration mechanism (7) in a rotating manner, the vibration mechanism (7) is connected with the inner part of the driving mechanism (2) in a rotating manner, and the driving mechanism (2) is arranged on the side wall of the storage mechanism (4); the rubber blocks (8) are respectively arranged in the storage mechanism (4) and the fixing mechanism (6); the storage mechanism (4) comprises a clamping groove (41), a protruding block (42), a first cylinder (43) and a second cylinder (44), the top surface of the support table (1) is fixedly connected with the first cylinder (43), and the interior of the first cylinder (43) is connected with the second cylinder (44) in a sliding manner; the bottom surface of the second cylinder (44) with the arc-shaped bottom side wall is symmetrically provided with the semicircular convex blocks (42), the bottom end of the first cylinder (43) is provided with the clamping groove (41) with the T-shaped cross section, and the inner part of the clamping groove (41) is connected with the convex blocks (42) in a sliding manner; the feeding mechanism (3) comprises a first valve (31), a feeding hopper (32) and a first corrugated pipe (33), the feeding hopper (32) is installed on the top surface of the first cylinder (43), and the first valve (31) is installed on the side wall of the feeding hopper (32); the bottom end of the feeding hopper (32) is provided with the first corrugated pipe (33), and the first corrugated pipe (33) penetrates through the first cylinder (43) and is connected with the top surface of the second cylinder (44); the blanking mechanism (5) comprises a drain pipe (51), a second valve (52), a second corrugated pipe (53), a fixing ring (54) and a groove (55), the groove (55) is formed in the supporting table (1), and one end of the groove (55) is communicated with the clamping groove (41); the groove (55) and the clamping groove (41) are internally provided with the second corrugated pipe (53) with the L-shaped side wall, the top end of the second corrugated pipe (53) is arranged at the top end of the second cylinder body (44), the bottom end of the second corrugated pipe (44) is provided with the drain pipe (51), and the side wall of the drain pipe (51) is provided with the second valve (52); the fixing ring (54) is arranged in the groove (55), and the fixing ring (54) is fixedly connected with the second corrugated pipe (53); the rubber blocks (8) are symmetrically arranged at the turning positions of the clamping grooves (41), and the side walls of the rubber blocks (8) are abutted against the side walls of the second corrugated pipes (53); the driving mechanism (2) comprises a motor (21) and a fixed box (22), the fixed box (22) is fixed on the side wall of the first cylinder (43), and the inside of the fixed box (22) is fixedly connected with the motor (21); the vibration mechanism (7) comprises a second spring (71), a connecting rod (72), a supporting frame (73), a crank (74), a connecting sleeve (75) and a clamping sleeve (76), the second spring (71) is symmetrically arranged between the inner side wall of the first cylinder (43) and the side wall of the second cylinder (44), the supporting frame (73) is arranged on the side wall of the top end of the second cylinder (44), and the supporting frame (73) is rotatably connected with the connecting rod (72); the connecting rod (72) is rotationally connected with the connecting sleeve (75), the connecting sleeve (75) is rotationally connected with the crank (74), and the crank (74) is rotationally connected with the motor (21); the clamping sleeves (76) are symmetrically arranged on the side wall of the crank (74), and the connecting sleeve (75) is arranged between the two clamping sleeves (76); the fixing mechanism (6) comprises a fixing rod (61), a fixing sleeve (62), a first spring (63), sliding grooves (64) and balls (65), the side wall of the top end of the second cylinder (44) is fixedly connected with the fixing sleeve (62), a plurality of sliding grooves (64) are symmetrically arranged in two ends of the fixing sleeve (62), the fixing rod (61) is connected in the sliding grooves (64) in a sliding mode, and the first spring (63) is arranged between one end of the fixing rod (61) and one end of each sliding groove (64); the other end of the fixed rod (61) is rotatably connected with the ball (65), and the ball (65) is abutted against the inner side wall of the first barrel (43); the shortest vertical distance between the lateral wall edge of fixed cover (62) and the inside wall of first barrel (43) is greater than the height of crank (74), just the other end symmetry installation of spout (64) block rubber (8), block rubber (8) conflict the lateral wall of dead lever (61).
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| CN201811236925.0A CN109321632B (en) | 2018-10-23 | 2018-10-23 | RNA cell molecule inspection method |
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| CN201811236925.0A CN109321632B (en) | 2018-10-23 | 2018-10-23 | RNA cell molecule inspection method |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1978040A (en) * | 2005-12-07 | 2007-06-13 | 埃佩多夫股份公司 | Apparatus for shaking sample containers |
| CN101762585A (en) * | 2009-05-05 | 2010-06-30 | 孔兵 | Cell morphology microscopic examination device and method based on staining technology |
| CN205078669U (en) * | 2015-09-28 | 2016-03-09 | 苏杰 | Shock absorber of lamp |
| CN107083328A (en) * | 2017-05-10 | 2017-08-22 | 江苏大学 | A kind of cell concentration uniformity control method and device |
| CN107377041A (en) * | 2017-06-14 | 2017-11-24 | 郑州永泰澳新商贸有限公司 | A kind of chemical experiment test tube support with concussion function |
| CN207430189U (en) * | 2017-11-02 | 2018-06-01 | 任惠萍 | A kind of cell evenly mixing device |
| CN207750443U (en) * | 2018-01-19 | 2018-08-21 | 安徽省农业科学院水产研究所 | A kind of aquatic products refrigerator |
| CN207850518U (en) * | 2017-12-20 | 2018-09-11 | 天津港国际物流发展有限公司 | A kind of inspection and quarantine meausring apparatus |
| CN207838863U (en) * | 2017-11-27 | 2018-09-11 | 绍兴柯桥天宇印染有限公司 | A kind of printing and dyeing assistant mixing arrangement easy to use |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080011564A1 (en) * | 2006-07-11 | 2008-01-17 | Wan-Lai Chen | Damping Structure for Drawers |
-
2018
- 2018-10-23 CN CN201811236925.0A patent/CN109321632B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1978040A (en) * | 2005-12-07 | 2007-06-13 | 埃佩多夫股份公司 | Apparatus for shaking sample containers |
| CN101762585A (en) * | 2009-05-05 | 2010-06-30 | 孔兵 | Cell morphology microscopic examination device and method based on staining technology |
| CN205078669U (en) * | 2015-09-28 | 2016-03-09 | 苏杰 | Shock absorber of lamp |
| CN107083328A (en) * | 2017-05-10 | 2017-08-22 | 江苏大学 | A kind of cell concentration uniformity control method and device |
| CN107377041A (en) * | 2017-06-14 | 2017-11-24 | 郑州永泰澳新商贸有限公司 | A kind of chemical experiment test tube support with concussion function |
| CN207430189U (en) * | 2017-11-02 | 2018-06-01 | 任惠萍 | A kind of cell evenly mixing device |
| CN207838863U (en) * | 2017-11-27 | 2018-09-11 | 绍兴柯桥天宇印染有限公司 | A kind of printing and dyeing assistant mixing arrangement easy to use |
| CN207850518U (en) * | 2017-12-20 | 2018-09-11 | 天津港国际物流发展有限公司 | A kind of inspection and quarantine meausring apparatus |
| CN207750443U (en) * | 2018-01-19 | 2018-08-21 | 安徽省农业科学院水产研究所 | A kind of aquatic products refrigerator |
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| CN109321632A (en) | 2019-02-12 |
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