CN109321433A - A kind of cell culture bags suspension pumping equipment - Google Patents
A kind of cell culture bags suspension pumping equipment Download PDFInfo
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- CN109321433A CN109321433A CN201811483301.9A CN201811483301A CN109321433A CN 109321433 A CN109321433 A CN 109321433A CN 201811483301 A CN201811483301 A CN 201811483301A CN 109321433 A CN109321433 A CN 109321433A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 61
- 239000000725 suspension Substances 0.000 title claims abstract description 28
- 238000005086 pumping Methods 0.000 title claims abstract description 18
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000006073 displacement reaction Methods 0.000 claims description 7
- 239000000945 filler Substances 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 3
- 239000002344 surface layer Substances 0.000 claims description 3
- 230000001360 synchronised effect Effects 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 2
- 230000008602 contraction Effects 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 230000002093 peripheral effect Effects 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 36
- 239000000463 material Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 16
- 238000000034 method Methods 0.000 description 13
- 238000011017 operating method Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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Abstract
The invention discloses a kind of cell culture bags to hang pumping equipment, belongs to technical field of cell culture.It mainly includes pedestal, roller, rotary part, retraction member, rotation stop component, suspension parts, by the way that cell culture bags are suspended on roller, with the gradually outflow of cell liquid, rotating cylinder, so that the part of the drained liquid of cell culture bags is gradually wrapped up on roller, the discharge of cell liquid is realized.Portable cell culture bags are inconvenient outwardly against caused by when solving collection cell liquid of the existing technology, easy damaged cells culture bag causes leakage, cell culture bags bottom residual cell liquid to be not easy to be extruded, increase the drawbacks of cell acquisition time, wasting manpower and material resources.Be not easy to be damaged with easy to operate, cell culture bags, cell liquid discharge thoroughly, save the advantage of manpower and acquisition time.
Description
Technical field
The present invention relates to a kind of cell culture auxiliary devices, and in particular to a kind of cell culture bags suspension pumping equipment.
Background technique
Cell culture refers to simulated in vivo environment in vitro, makes cells survival, growth, breeding and maintains primary structure and function
Energy.It is widely applied in biological study, medical research and application field, becomes modern biology, medicine drug development and application
Important means.
Common cell culture vessel is Tissue Culture Dish, Tissue Culture Flask, but when the cell concentration for needing to cultivate is larger, carefully
The drawbacks of born of the same parents' culture dish, Tissue Culture Flask are big in the presence of opening, leakproofness is poor, internal cell liquid is easy spilling, moreover, for suspending
The culture of cell, requires the Air-cleaning technique in culture vessel high, is statically placed in Tissue Culture Dish, is trained in Tissue Culture Flask
Feeding mode is also easy to produce the big problem of cell aggregation, local cell density, is unfavorable for air exchange, cell Proliferation is caused to be not achieved
Desired effect, thus to begin to use cell culture bags made of the high molecular material of gas-permeable, liquid-impermeable larger to carry out by people later
The culture of amount, suspension growth cell.The use of cell culture bags solves the problems, such as that ventilation volume is small and cell proliferation rate is slow,
And good airproof performance.
However, after cell culture period, mostly experiment operator portable cell culture bags when collecting cell liquid
It is collected outwardly against cell liquid, this method is inconvenient, and is easy to cause the breakage and leakage of cell culture bags in operation
Liquid, during cell is collected, the cell in cell culture bags has many colonies at bag bottom, brings barrier to experiment operator
Hinder, increases cell contamination probability and cell acquisition time, wasting manpower and material resources.And dedicated for solve the method for this drawback,
Device or equipment, do not see application largely also at present.
Summary of the invention
The present invention provides a kind of cell culture bags to hang pumping equipment, it is therefore intended that solves the problems, such as above-mentioned: thin
After born of the same parents' cultivation cycle, portable cell culture bags are inconvenient outwardly against caused by, easy damaged cells when collecting cell liquid
Culture bag causes leakage, cell culture bags bottom residual cell liquid to be not easy to be extruded, increase cell acquisition time, wasting manpower and material resources
The drawbacks of.
The present invention is achieved through the following technical solutions: a kind of cell culture bags suspension pumping equipment, including pedestal, rolling
Cylinder, rotary part, retraction member, rotation stop component, suspension parts, roller pass through drum shaft and the left pillar axis hole of pedestal and right pillar
The axis hole level of being connected is fixed on pedestal, is joined across the roller axial end of right pillar axis hole and a rotary part synchronous rotary
It connects, the right pillar axle hole position of right pillar lateral surface is provided with a rotation stop component, retraction member use is provided on rotary part
To realize that rotary part retracts and chimeric with rotation stop component automatically, the cylindrical surface of roller is equipped with one and adjusts the distance adjustable suspension part
Part.
Pedestal is by rectangular base plate and is fixed on the vertical of substrate left and right sides edge, symmetrical left pillar and right branch
Column is constituted, and left pillar is provided with coaxial isodiametric left pillar axis hole, right pillar axis hole close to top with right pillar.
Rotation stop component is provided with the hollow cylinder with right pillar axis hole equal diameter through hole, and hollow cylinder for a middle part
One end face surface layer is provided with that mutual angle is equal, the end face center of circle is directed toward in direction along its end face radial direction at its circular ring shape position
Rotation stop groove, hollow cylinder other end is close to right pillar lateral surface and is fixed, and the axle center of hollow cylinder and right pillar
Axis hole is coaxial.
Cylinder body is a cylindrical body, reduces at its both ends close to endface diameter and shrinks that form end coaxially equal straight inwards
The drum shaft of diameter is the rectangular item of square integrally formed with a cross section on the end face of the drum shaft coupled with right pillar,
And the center of its cross section square is overlapped with drum shaft axle center, the circumradius of cross section square is less than the half of drum shaft
Diameter, is provided with a strip-shaped groove along roller axial direction in cylindrical cylinder, groove cross section be on one bottom be less than bottom etc.
Waist is trapezoidal.
The length of roller is less than horizontal distance, the length of roller both ends drum shaft between the left pillar of pedestal and right post inboard face
So that roller be installed on the lateral surface of both ends drum shaft when on the left pillar of pedestal and right pillar just respectively with left pillar and right branch
Column lateral surface is coplanar, the length of the rectangular item in roller axial end coupled with right pillar and the hollow cylinder of rotation stop component
Through hole length it is identical.
A roller shaft filler washer is respectively covered on the drum shaft at roller both ends, when the thickness of washer is mounted on the base roller
Horizontal direction displacement will not occur, and the lateral surface of roller both ends drum shaft is just respectively and on the outside of the left pillar of pedestal and right pillar
Face is coplanar.
Rotary part by cylindrical rotary shaft, rotary shaft cylindrical surface along radial direction protrusion radial bars, with rotation
Axial cylindrical face connects and constitutes along the handlebar of rotary shaft radial direction, the rotating handle connecting with handlebar outer end, outside rotary shaft
Diameter is smaller than the internal diameter of rotation stop component through hole and difference is less than 2 millimeters, and rotary shaft is run through along axis direction by a square hole, should
The side length of square hole is greater than the side length and difference of shape sliver transvers section square above roller axial end less than 2 millimeters, square
Hole axle center is overlapped with rotary shaft axle center, the complete kissing of rotation stop groove of radial bars quantity and angular distribution and rotation stop component end face
It closes, the diameter of radial bars is less than the width of rotation stop component end face rotation stop groove and length is less than the length of rotation stop groove, rotates hand
The connection of the end vertical of handle and handlebar, and rotating handle direction is axially parallel with rotary shaft.
Suspension parts are made of sliding block, L-type hanged hook, positioning bolt, and sliding block is quadrangular frustum pyramid shaped, one end of L-type hanged hook
Vertical with sliding block upper bottom surface to be fixedly connected, a threaded hole extends vertically through sliding block, the screw thread and spiral shell of positioning bolt by sliding block upper bottom surface
The screw thread of pit matches, and positioning bolt is screwed by the threaded bores of sliding block upper bottom surface and passes through spiral shell of the sliding block from sliding block bottom surface
Pit aperture is pierced by, and sliding block bottom is face-down, upper bottom surface is embedded in upward in the groove in cylindrical cylinder, hanged hook and positioning spiral shell
Bolt cap is placed on outside groove.
Retraction member is by spring and is fixed on the spring compressor of spring one end and constitutes.
Specific connection type between each component are as follows: roller has the drum shaft of rectangular one end to penetrate in right pillar axis hole,
The drum shaft of the roller other end penetrates in left pillar axis hole, and the rotary shaft of rotary part one end contrary with rotating handle is inserted
Square hole in the through hole for entering rotation stop component, and among rotary shaft is placed on the rectangular item periphery of roller axial end, retraction portion
One end of the unlocked spring compressor of the spring of part penetrates rotary shaft square hole by outside and is fixedly connected with the outer end face of rectangular item, bullet
The diameter of spring cap is greater than rotary shaft square hole inscribed circle diameter and is blocked in outside square hole, and tensioned will be in axial rotary
It draws so that the radial bars in rotary shaft are fallen into the rotation stop groove of rotation stop component end face.
The present invention, a kind of cell culture bags suspension pumping equipment, specifically used method are as follows:
(1) dormant state.When the device when not in use, hand grasps rotating handle and gently dials the radial bars so that rotary part outward
Until just all coming out from the rotation stop groove of rotation stop component end face, the dynamics for keeping this to pull out outward makes rotary part exist
It is not subjected to displacement in horizontal direction, then rotates rotating handle and drive rotary shaft rotation, so that the groove location on roller rotates
The rotational angle for stopping operating when leaning on upper bit, and finely tuning rotary shaft to the leading flank on its cylindrical surface to discharge rotating part
After the dynamics that part pulls out outward, rotary part is subjected to displacement inwards under the action of retraction member, so that radial bars are fairly fallen in only
In the rotation stop groove of rotation member end face；
(2) use process.When use, first have to rotate outward the positioning bolt on suspension parts with hand so that sliding block with
Groove unclamps, and stirs the suspension parts in cylinder gap and makes the distance of two suspension parts greater than the width of cell culture bags
Degree, the then positioning bolt of rotating suspension component inwards, so that two sides of sliding block are pressed on groove side, so that its
Position is fixed up, by cell culture bags mouth down bottom upward, two ropes of bottom system, two rope other ends lean outward respectively
It is tiltedly tied up on the hanged hook of two suspension parts in herringbone, opens cell culture sack, cell liquid is begun to flow out under entrance
In the collection vessel of side, with the decline of liquid level in the outflow of cell liquid, cell culture bags, in time, suitably, intermittently use
Hand rotates rotary part, so that the part of the drained liquid of cell culture bags is constantly wrapped up in onto roller, until in cell culture bags
Cell liquid be all discharged, then then reverse rotation component passes through dormant state so that cell culture bags are removed from roller
Method in operating procedure the groove location on roller rotate to its cylindrical surface leading flank by upper bit, wait make next time
With.Rotation rotary part and the method for the operation that stops operating, be by outside as described in dormant state operating procedure every time
Dial dynamics and discharge the dynamics make rotary part radial bars realize separated with the rotation stop groove of rotation stop component end face with
And it is chimeric.
The present invention has the advantage that
(1) easy to operate.Cell liquid is discharged using the present apparatus, avoids the operation of manual compression cell culture bags, hangs over this dress
The voluntarily outflow of cell liquid can be realized on the suspension parts set, it is simple to operate；
(2) a possibility that cell culture bags are damaged substantially reduces.The behaviour to compare with traditional manual expression cell culture bags
Make, cell culture bags are hung on the device and are voluntarily flowed out by cell liquid self gravity, cell culture bags is avoided and is squeezed
When the risk being scratched, crushed；
(3) cell liquid discharge is thorough.The method of traditional manual expression cell culture bags discharge cell liquid, in cell culture bags
Cell has more colony at bag bottom, and cell liquid discharge is caused to be not thorough.And on the contrary, cell culture bags are fallen after using the device
It sets, as cell liquid is gradually flowed out from sack, the liquid level in bag is gradually reduced from bottom, and the part of the drained liquid of sack is gradually wrapped up in
Onto roller, drain is very thorough, does not remain in bag；
(4) manpower and acquisition time are saved.Due to the use of the device, the operation of manual compression cell culture bags has been saved, has been saved
Manpower.Meanwhile traditional manual operation needs to repeatedly extrude in cell culture bags, especially cell culture bags and remains cell liquid
And need by remaining cell liquid squeeze out when, more need to repeatedly extrude cell culture bags, will lead in this way cell liquid discharge or
The time of collection extends, and the use of the device, cell liquid are gradually flowed out from the sack of lower end, and the liquid level in bag is gradually reduced,
It not will lead to washing lotion discharge to be not thorough and remain, the time for shortening cell liquid discharge or collecting.
Detailed description of the invention
The present invention will be further described in detail with reference to the accompanying drawings and detailed description.
Fig. 1 is embodiment of the present invention base schematic diagram.
Fig. 2 is enlarged diagram at A in Fig. 1.
Fig. 3 is that Fig. 2 shows that structure is cut away the schematic diagram that half shows internal structure by middle part.
Fig. 4 is embodiment of the present invention roller schematic elevation view.
Fig. 5 is embodiment of the present invention roller schematic side view.
Fig. 6 is embodiment of the present invention suspension parts schematic diagram.
Fig. 7 is embodiment of the present invention rotary part schematic elevation view.
Fig. 8 is embodiment of the present invention rotary part schematic side view.
Fig. 9 is embodiment of the present invention retraction member schematic diagram.
Figure 10 is embodiment of the present invention roller, right pillar, rotary part, retraction member assembling mode schematic diagram.
Figure 11 is enlarged diagram at B in Figure 10.
Figure 12 is that Figure 11 shows that structure is cut away the schematic diagram that half shows internal structure by middle part.
Figure 13 is the schematic diagram that embodiment of the present invention assembles and rotary part is extracted.
Figure 14 is enlarged diagram at C in Figure 13.
Figure 15 is that Figure 14 shows that structure is cut away the schematic diagram that half shows internal structure by middle part.
Figure 16 is that Figure 13 shows that structure cuts away half by middle part and shows schematic diagram of internal structure.
Figure 17 is the schematic side view that Figure 13 shows structure.
Figure 18 is enlarged diagram at D in Figure 17.
Figure 19 is enlarged diagram at E in Figure 17.
Figure 20 is the partial schematic diagram that embodiment of the present invention assembles and rotary part retracts.
Figure 21 is the schematic side view that Figure 20 shows structure.
Figure 22 is that Figure 20 shows that structure is cut away the schematic diagram that half shows internal structure by middle part.
In figure: the left pillar of 101-, 102- bottom plate, the right pillar of 103-, the left pillar axis hole of 104-, 105- rotation stop component, 106-
Right pillar axis hole, 201- roller, 202- drum shaft, 203- roller shaft filler washer, the rectangular item of 204-, 205- groove, 301- sliding block,
302-L type hanged hook, 303- positioning bolt, 4- rotary part, 401- radial bars, 402- square hole, 403- handlebar, 404-
Rotating handle, 405- rotary shaft, 5- retraction member, 501- spring, 502- spring compressor.
Specific embodiment
In conjunction with attached drawing, a kind of cell culture bags hang pumping equipment, including pedestal, roller, rotary part, retraction member,
Rotation stop component, suspension parts, roller are fixed on by drum shaft and the left pillar axis hole of pedestal and the right pillar axis hole level of being connected
On pedestal, the roller axial end across right pillar axis hole couples with a rotary part synchronous rotary, in the right right branch of pillar lateral surface
Axis of a cylinder hole position is provided with a rotation stop component, and a retraction member is provided on rotary part to realize that rotary part retracts automatically
And it is chimeric with rotation stop component, the cylindrical surface of roller is equipped with one and adjusts the distance adjustable suspension parts.
Pedestal is by rectangular base plate and is fixed on the vertical of substrate left and right sides edge, symmetrical left pillar and right branch
Column is constituted, and left pillar is provided with coaxial isodiametric left pillar axis hole, right pillar axis hole close to top with right pillar.
Rotation stop component is provided with the hollow cylinder with right pillar axis hole equal diameter through hole, and hollow cylinder for a middle part
One end face surface layer is provided with that mutual angle is equal, the end face center of circle is directed toward in direction along its end face radial direction at its circular ring shape position
Rotation stop groove, the quantity of rotation stop groove is 16, and hollow cylinder other end is close to right pillar lateral surface and is fixed, and hollow
The axle center of cylindrical body and right pillar axis hole are coaxial.
Cylinder body is a cylindrical body, reduces at its both ends close to endface diameter and shrinks that form end coaxially equal straight inwards
The drum shaft of diameter is the rectangular item of square integrally formed with a cross section on the end face of the drum shaft coupled with right pillar,
And the center of its cross section square is overlapped with drum shaft axle center, the circumradius of cross section square is less than the half of drum shaft
Diameter, is provided with a strip-shaped groove along roller axial direction in cylindrical cylinder, groove cross section be on one bottom be less than bottom etc.
Waist is trapezoidal.
The length of roller is less than horizontal distance, the length of roller both ends drum shaft between the left pillar of pedestal and right post inboard face
So that roller be installed on the lateral surface of both ends drum shaft when on the left pillar of pedestal and right pillar just respectively with left pillar and right branch
Column lateral surface is coplanar, the length of the rectangular item in roller axial end coupled with right pillar and the hollow cylinder of rotation stop component
Through hole length it is identical.
A roller shaft filler washer is respectively covered on the drum shaft at roller both ends, when the thickness of washer is mounted on the base roller
Horizontal direction displacement will not occur, and the lateral surface of roller both ends drum shaft is just respectively and on the outside of the left pillar of pedestal and right pillar
Face is coplanar.
Rotary part by cylindrical rotary shaft, rotary shaft cylindrical surface along radial direction protrusion radial bars, with rotation
Axial cylindrical face connects and constitutes along the handlebar of rotary shaft radial direction, the rotating handle connecting with handlebar outer end, outside rotary shaft
Diameter is smaller than the internal diameter of rotation stop component through hole and difference is 1 millimeter, and rotary shaft is run through along axis direction by a square hole, this is just
The side length and difference that the side length of square hole is greater than shape sliver transvers section square above roller axial end are 1 millimeter, square hole axle
The heart is overlapped with rotary shaft axle center, and radial bars quantity is 16 and mutual angle is identical, and radial bars are stopped with rotation stop component end face
Turn groove to match completely, the diameter of radial bars is less than the width of rotation stop component end face rotation stop groove and length is less than rotation stop groove
Length, the end vertical of rotating handle and handlebar connects, and rotating handle direction is axially parallel with rotary shaft.
Suspension parts are made of sliding block, L-type hanged hook, positioning bolt, and sliding block is quadrangular frustum pyramid shaped, one end of L-type hanged hook
Vertical with sliding block upper bottom surface to be fixedly connected, a threaded hole extends vertically through sliding block, the screw thread and spiral shell of positioning bolt by sliding block upper bottom surface
The screw thread of pit matches, and positioning bolt is screwed by the threaded bores of sliding block upper bottom surface and passes through spiral shell of the sliding block from sliding block bottom surface
Pit aperture is pierced by, and sliding block bottom is face-down, upper bottom surface is embedded in upward in the groove in cylindrical cylinder, hanged hook and positioning spiral shell
Bolt cap is placed on outside groove.
Retraction member is by spring and is fixed on the spring compressor of spring one end and constitutes.
Specific connection type between each component are as follows: roller has the drum shaft of rectangular one end to penetrate in right pillar axis hole,
The drum shaft of the roller other end penetrates in left pillar axis hole, and the rotary shaft of rotary part one end contrary with rotating handle is inserted
Square hole in the through hole for entering rotation stop component, and among rotary shaft is placed on the rectangular item periphery of roller axial end, retraction portion
One end of the unlocked spring compressor of the spring of part penetrates rotary shaft square hole by outside and is fixedly connected with the outer end face of rectangular item, bullet
The diameter of spring cap is greater than rotary shaft square hole inscribed circle diameter and is blocked in outside square hole, and tensioned will be in axial rotary
It draws so that the radial bars in rotary shaft are fallen into the rotation stop groove of rotation stop component end face.
Its application method is as follows:
(1) dormant state.When the device when not in use, hand grasps rotating handle and gently dials the radial bars so that rotary part outward
Until just all coming out from the rotation stop groove of rotation stop component end face, the dynamics for keeping this to pull out outward makes rotary part exist
It is not subjected to displacement in horizontal direction, then rotates rotating handle and drive rotary shaft rotation, so that the groove location on roller rotates
The rotational angle for stopping operating when leaning on upper bit, and finely tuning rotary shaft to the leading flank on its cylindrical surface to discharge rotating part
After the dynamics that part pulls out outward, rotary part is subjected to displacement inwards under the action of retraction member, so that radial bars are fairly fallen in only
In the rotation stop groove of rotation member end face；
(2) use process.When use, first have to rotate outward the positioning bolt on suspension parts with hand so that sliding block with
Groove unclamps, and stirs the suspension parts in cylinder gap and makes the distance of two suspension parts greater than the width of cell culture bags
Degree, the then positioning bolt of rotating suspension component inwards, so that two sides of sliding block are pressed on groove side, so that its
Position is fixed up, by cell culture bags mouth down bottom upward, two ropes of bottom system, two rope other ends lean outward respectively
It is tiltedly tied up on the hanged hook of two suspension parts in herringbone, opens cell culture sack, cell liquid is begun to flow out under entrance
In the collection vessel of side, with the decline of liquid level in the outflow of cell liquid, cell culture bags, in time, suitably, intermittently use
Hand rotates rotary part, so that the part of the drained liquid of cell culture bags is constantly wrapped up in onto roller, until in cell culture bags
Cell liquid be all discharged, then then reverse rotation component passes through dormant state so that cell culture bags are removed from roller
Method in operating procedure the groove location on roller rotate to its cylindrical surface leading flank by upper bit, wait make next time
With.Rotation rotary part and the method for the operation that stops operating, be by outside as described in dormant state operating procedure every time
Dial dynamics and discharge the dynamics make rotary part radial bars realize separated with the rotation stop groove of rotation stop component end face with
And it is chimeric.
Claims (10)
1. a kind of cell culture bags hang pumping equipment, which is characterized in that including pedestal, roller, rotary part, retraction member,
Rotation stop component, suspension parts, roller are fixed on by drum shaft and the left pillar axis hole of pedestal and the right pillar axis hole level of being connected
On pedestal, the roller axial end across right pillar axis hole couples with a rotary part synchronous rotary, in the right right branch of pillar lateral surface
Axis of a cylinder hole position is provided with a rotation stop component, and a retraction member is provided on rotary part to realize that rotary part retracts automatically
And it is chimeric with rotation stop component, the cylindrical surface of roller is equipped with one and adjusts the distance adjustable suspension parts.
2. a kind of cell culture bags as described in claim 1 hang pumping equipment, which is characterized in that the pedestal is by rectangle
Bottom plate and vertical, the symmetrical left pillar being fixed at substrate left and right sides edge and right pillar are constituted, left pillar and right
Pillar is provided with coaxial isodiametric left pillar axis hole, right pillar axis hole close to top.
3. a kind of cell culture bags as described in claim 1 hang pumping equipment, which is characterized in that the rotation stop component is
One middle part is provided with the hollow cylinder with right pillar axis hole equal diameter through hole, and one end face surface layer of hollow cylinder is along its end
Radius surface direction is provided with the rotation stop groove that mutual angle is equal, the end face center of circle is directed toward in direction, open circles at its circular ring shape position
Cylinder other end is close to right pillar lateral surface and is fixed, and the axle center of hollow cylinder and right pillar axis hole are coaxial.
4. a kind of cell culture bags as described in claim 1 hang pumping equipment, which is characterized in that the cylinder body is
One cylindrical body reduces contraction inwards close to endface diameter at its both ends and forms end coaxially isodiametric drum shaft, with right branch
Column connection drum shaft end face on integrally formed with a cross section be square rectangular item, and its cross section square
Center is overlapped with drum shaft axle center, and the circumradius of cross section square is less than the radius of drum shaft, is opened in cylindrical cylinder
There is a strip-shaped groove along roller axial direction, groove cross section is the isosceles trapezoid that bottom is less than bottom on one.
5. a kind of cell culture bags as claimed in claim 4 hang pumping equipment, which is characterized in that the roller, length
Degree is less than horizontal distance, the length of roller both ends drum shaft between the left pillar of pedestal and right post inboard face and roller is made to be installed on bottom
The lateral surface of both ends drum shaft is just coplanar with left pillar and right pillar lateral surface respectively when on the left pillar of seat and right pillar, with
The length of rectangular item in the roller axial end of right pillar connection is identical through hole length as the hollow cylinder of rotation stop component.
6. a kind of cell culture bags as claimed in claim 4 hang pumping equipment, which is characterized in that the roller, two
A roller shaft filler washer is respectively covered on the drum shaft at end, and level side will not occur when the thickness of washer is mounted on the base roller
To displacement, and the lateral surface of roller both ends drum shaft is just coplanar with the left pillar of pedestal and right pillar lateral surface respectively.
7. a kind of cell culture bags as described in claim 1 hang pumping equipment, which is characterized in that the rotary part by
It is connect in cylindrical rotary shaft, rotary shaft cylindrical surface along the radial bars of radial direction protrusion, with rotary shaft cylindrical surface and along rotation
The handlebar of shaft radial direction, the rotating handle connecting with handlebar outer end are constituted, and the outer diameter of rotary shaft is than rotation stop component through hole
Internal diameter it is small and difference is less than 2 millimeters, rotary shaft is run through along axis direction by a square hole, and the side length of the square hole is greater than
The side length and difference of shape sliver transvers section square are less than 2 millimeters above roller axial end, square hole axle center and rotary shaft axle center
It is overlapped, radial bars quantity and angular distribution match completely with the rotation stop groove of rotation stop component end face, and the diameter of radial bars is less than
The width and length of rotation stop component end face rotation stop groove are less than the length of rotation stop groove, the end vertical of rotating handle and handlebar
Connection, and rotating handle direction is axially parallel with rotary shaft.
8. a kind of cell culture bags as described in claim 1 hang pumping equipment, which is characterized in that the suspension parts by
Sliding block, L-type hanged hook, positioning bolt are constituted, and sliding block is quadrangular frustum pyramid shaped, and one end of L-type hanged hook is vertical with sliding block upper bottom surface solid
Fixed connection, a threaded hole extend vertically through sliding block by sliding block upper bottom surface, and the screw thread of positioning bolt and the screw thread of threaded hole match, fixed
Position bolt is screwed by the threaded bores of sliding block upper bottom surface and passes through sliding block to be pierced by from the threaded bores of sliding block bottom surface, sliding block bottom
Down, upper bottom surface is embedded in upward in the groove in cylindrical cylinder, and hanged hook and positioning bolt cap are placed on outside groove.
9. a kind of cell culture bags as described in claim 1 hang pumping equipment, which is characterized in that the retraction member by
Spring and the spring compressor for being fixed on spring one end are constituted.
10. a kind of cell culture bags as described in claims 1 or 2 or 3 or 4 or 7 or 9 hang pumping equipment, which is characterized in that
The structure, roller have the drum shaft of rectangular one end to penetrate in right pillar axis hole, and the drum shaft of the roller other end penetrates a left side
In pillar axis hole, in the through hole of the rotary shaft of rotary part insertion rotation stop component in one end contrary with rotating handle, and
The rectangular item that square hole among rotary shaft is placed on roller axial end is peripheral, and the one of the unlocked spring compressor of the spring of retraction member
End penetrates rotary shaft square hole by outside and is fixedly connected with the outer end face of rectangular item, and it is square that the diameter of spring compressor is greater than rotary shaft
Shape hole inscribed circle diameter is blocked in outside square hole, and axial rotary lira falls the radial bars in rotary shaft by tensioned
In the rotation stop groove for entering rotation stop component end face.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811483301.9A CN109321433A (en) | 2018-12-06 | 2018-12-06 | A kind of cell culture bags suspension pumping equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811483301.9A CN109321433A (en) | 2018-12-06 | 2018-12-06 | A kind of cell culture bags suspension pumping equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109321433A true CN109321433A (en) | 2019-02-12 |
Family
ID=65256339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811483301.9A Pending CN109321433A (en) | 2018-12-06 | 2018-12-06 | A kind of cell culture bags suspension pumping equipment |
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| CN (1) | CN109321433A (en) |
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| CN201010796Y (en) * | 2006-12-12 | 2008-01-23 | 刘诚 | Cleaning and whirling mechanism of novel roller washing machine |
| CN204874548U (en) * | 2015-07-21 | 2015-12-16 | 湖北华赛生物医药技术有限公司 | Cell culture bag with adjustable |
| CN205576162U (en) * | 2016-04-01 | 2016-09-14 | 斯坦姆（天津）细胞工程技术有限公司 | Modified cell culture bag |
| WO2016190312A1 (en) * | 2015-05-25 | 2016-12-01 | ニプロ株式会社 | Culturing device |
| CN209193953U (en) * | 2018-12-06 | 2019-08-02 | 山西正一康生物科技有限公司 | A kind of cell culture bags suspension pumping equipment |
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2018
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201010796Y (en) * | 2006-12-12 | 2008-01-23 | 刘诚 | Cleaning and whirling mechanism of novel roller washing machine |
| WO2016190312A1 (en) * | 2015-05-25 | 2016-12-01 | ニプロ株式会社 | Culturing device |
| CN204874548U (en) * | 2015-07-21 | 2015-12-16 | 湖北华赛生物医药技术有限公司 | Cell culture bag with adjustable |
| CN205576162U (en) * | 2016-04-01 | 2016-09-14 | 斯坦姆（天津）细胞工程技术有限公司 | Modified cell culture bag |
| CN209193953U (en) * | 2018-12-06 | 2019-08-02 | 山西正一康生物科技有限公司 | A kind of cell culture bags suspension pumping equipment |
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